CN219334575U

CN219334575U - Tissue separation device

Info

Publication number: CN219334575U
Application number: CN202320007911.1U
Authority: CN
Inventors: 董远; 陈颂; 王道锋; 康晨; 季霁
Original assignee: Henan Qianze Biotechnology Co ltd
Current assignee: Henan Qianze Biotechnology Co ltd
Priority date: 2023-01-04
Filing date: 2023-01-04
Publication date: 2023-07-14
Anticipated expiration: 2033-01-04

Abstract

The utility model relates to a tissue separation device, which comprises a base, an outer cover cylinder, an inner screen cylinder, a cover body, a stirring assembly and a centrifugal assembly, wherein the upper side of the base is provided with a mounting groove, the upper end and the lower end of the outer cover cylinder are both open, the lower end of the outer cover cylinder is arranged in the mounting groove in a threaded manner, the upper end of the inner screen cylinder is open, the lower end of the inner screen cylinder is sealed, the inner screen cylinder is positioned in the outer cover cylinder, the outer side wall of the inner screen cylinder is provided with a plurality of screen holes, and the cover body is buckled on the upper side of the outer cover cylinder and is in threaded connection with the upper end of the outer cover cylinder; the stirring assembly is located on the cover body and comprises a stirring motor, a stirrer and a stirring shaft, the centrifugal assembly is located on the lower side of the inner screen cylinder and used for driving the inner screen cylinder to rotate, the stirring assembly comprises a centrifugal motor and a centrifugal column, the lower side of the cover body is rotationally provided with a rotary compression ring through a second bearing, and the rotary compression ring is used for propping against the upper end of the inner screen cylinder and rotating along with the inner screen cylinder. The centrifugal function and the stirring function are mutually independent, and all parts are detachable, so that the utility model has the advantages of full and thorough crushing and convenient cleaning.

Description

Tissue separation device

Technical Field

The utility model relates to the technical field of biological equipment, in particular to a tissue separation device.

Background

Mesenchymal Stem Cells (MSC) are multipotent stem cells, have all the commonalities of stem cells, namely self-renewal and multi-directional differentiation, and are clinically applied to various blood system diseases, cardiovascular diseases, autoimmune diseases and the like to make a great breakthrough.

The Chinese patent document of the publication No. CN210620768U discloses a separation device for separating placenta wall decidua and placenta chorion, which comprises a cover body, an outer cover, a screen body, a stirrer, a bottom column and a base, wherein the outer cover is fixed on the base, the screen body is rotatably arranged in the outer cover, the base comprises a rotating motor connected with the screen body to drive the screen body to rotate, the inside of the screen body is provided with a stirrer which synchronously rotates along with the screen body to stir and cut placenta tissues, the placenta tissues are placed in the screen body in the use process, the rotating motor is started, the stirrer cuts the placenta tissues, the screen body rotates to throw the placenta wall decidua out of the screen body through sieve holes in the screen body by utilizing centrifugal force, and the placenta chorion is remained in the screen body due to the large thickness. The above-described separation device conveniently separates the placental wall decidua from the placental chorion using centrifugal force, but still has the following disadvantages: the stirrer rotates synchronously with the sieve body and the materials, and the stirrer breaks the materials insufficiently and has poor effect; the dustcoat, screen frame are all fixed on the base, can't dismantle during the washing, have the clean dead angle easily, wash inconvenient.

Disclosure of Invention

The utility model aims to provide a tissue separation device which can solve the technical problems that a stirrer of the existing separation device for separating placenta wall decidua and placenta chorion is not thorough in breaking materials and inconvenient in cleaning the device.

The utility model provides the following technical scheme: the tissue separation device comprises a base, an outer cover cylinder, an inner screen cylinder, a cover body, a stirring assembly and a centrifugal assembly, wherein the upper side of the base is provided with a mounting groove, the upper end and the lower end of the outer cover cylinder are both open, the lower end of the outer cover cylinder is arranged in the mounting groove in a threaded manner, the upper end of the inner screen cylinder is open, the lower end of the inner screen cylinder is blocked, the inner screen cylinder is coaxially positioned in the outer cover cylinder, the outer side wall of the inner screen cylinder is provided with a plurality of screen holes, and the cover body is buckled on the upper side of the outer cover cylinder and is in threaded connection with the upper end of the outer cover cylinder;

the stirring assembly is positioned on the cover body and comprises a stirring motor, a stirrer and a stirring shaft, the stirring motor is positioned on the outer side of the cover body, a plurality of stirring blades are arranged on the stirrer and positioned at the lower end of the stirring shaft, the upper end of the stirring shaft penetrates out of the cover body and is connected with the output end of the stirring motor, and the cover body is rotationally connected with the stirring shaft through a first bearing; the centrifugal component is positioned at the lower side of the inner screen cylinder and used for driving the inner screen cylinder to rotate, and comprises a centrifugal motor and a centrifugal column, wherein the centrifugal motor is positioned in the base, the lower end of the centrifugal column is connected with the output end of the centrifugal motor, the upper end of the centrifugal column extends out of the base, the upper end of the centrifugal column is provided with a rotation stopping block, the bottom of the inner screen cylinder is provided with a rotation stopping groove for the rotation stopping block to be inserted and matched with the rotation stopping block in a rotation stopping way, and the centrifugal column is matched with the base in a rotation sealing way; the rotary press ring is rotatably assembled on the lower side of the cover body through the second bearing and is used for propping against the upper end of the inner screen cylinder and rotating along with the inner screen cylinder.

The beneficial effects are that: the process of separating placenta chorion and placenta wall decidua by using the tissue separating device comprises the steps of adding placenta chorion with placenta wall decidua into an inner screen cylinder, sequentially installing an outer screen cylinder, an inner screen cylinder and a cover body, turning on a stirring motor and a centrifugal motor, driving a stirring shaft and a stirrer to rotate by the stirring motor, breaking placenta tissues to separate the placenta chorion from the placenta wall decidua, driving a centrifugal column to rotate by the rotation of the centrifugal motor, driving the inner screen cylinder to rotate by a rotation stopping block by the centrifugal column, and throwing and collecting the placenta wall decidua in the inner screen cylinder through screen holes under the centrifugal effect to achieve the separation effect; by combining the above processes and the device structure, the stirring component is arranged on the cover body, the centrifugal component is arranged on the base, and the stirring and centrifugal rotation are respectively and independently acted by the stirring motor and the centrifugal motor, so that the stirrer and the materials generate relative motion, the materials are crushed thoroughly, and the separation effect of placenta chorion and placenta wall decidua is good; the outer cover cylinder is detachably matched with the base, the inner screen cylinder is detachably matched with the base, the rotation stopping groove and the rotation stopping block are used for driving the inner screen cylinder to rotate, the cover body is provided with a rotary compression ring for press-mounting the inner screen cylinder, the inner screen cylinder is guaranteed to stably and centrifugally rotate on the basis of being detachable, the structure is novel and reasonable, and the inner screen cylinder, the outer cover cylinder, the base and the cover body are all detachable and are convenient to clean independently, dead angles are difficult to clean, and cleaning is convenient.

Further, a limiting groove for the upper end of the inner screen drum to extend in is formed in the lower side of the rotary compression ring.

The beneficial effects are that: the upper end of the inner screen cylinder is clamped into the limiting groove, so that the vertical direction position of the inner screen cylinder is limited by the press fit effect, the radial limiting effect can be achieved, and the inner screen cylinder is prevented from shaking or taking off the groove in the rotation process.

Further, the stirrer is provided with at least two.

The beneficial effects are that: stirring is more complete, promotes crushing effect.

Further, through third bearing, the rotatory sealing washer rotary seal cooperation of base between centrifugal column and the base, the last sealing convex ring that sets up in the downside that ends the commentaries on classics piece of centrifugal column, sealing convex ring downside is equipped with and is used for with the rotatory sealing washer of base rotary seal complex convex ring.

The beneficial effects are that: the lower side of the sealing convex ring is provided with a convex ring rotary sealing ring, and a base rotary sealing ring is arranged between the centrifugal column and the base, so that the placenta wall decidua or liquid is prevented from leaking, and the leakage-proof effect is good.

Further, at least two convex ring rotary sealing rings are arranged.

The beneficial effects are that: and (5) multistage sealing, and improving the rotary sealing grade.

Drawings

FIG. 1 is a schematic view of a tissue separation device according to embodiment 1 of the present utility model;

reference numerals: 1-a base; 2-a housing cylinder; 3-an inner screen drum; 4-a cover; 5-sieve pores; 6, a stirring motor; 7, a stirring shaft; 8-a stirrer; 9-centrifuging the motor; 10-a centrifugal column; 11-a rotation stopping block; 12-a rotation stopping groove; 13-sealing a convex ring; 14-a convex ring rotating sealing ring; 15-a first bearing; 16-a second bearing; 17-rotating the compression ring; 18-a third bearing; 19-base rotary seal ring.

Detailed Description

The present utility model will be described in detail with reference to the accompanying drawings.

Specific example 1 of a tissue separation device of the present utility model:

the structure of the tissue separation device is shown in fig. 1, and mainly comprises a base 1, an outer cover cylinder 2, an inner screen cylinder 3, a cover body 4, a stirring assembly arranged on the cover body 4 and a centrifugal assembly arranged on the base 1, wherein the upper end and the lower end of the outer cover cylinder 2 are both open, a mounting groove is formed in the upper side of the base 1, threads of the lower end of the outer cover cylinder 2 are mounted in the mounting groove, and the upper end of the outer cover cylinder 2 is in threaded connection with the cover body 4 and is used for plugging the upper end of the outer cover cylinder 2. The inner screen cylinder 3 is coaxially arranged inside the outer screen cylinder 2, a plurality of screen holes 5 for breaking placenta wall decidua to pass through are formed in the outer side wall of the outer screen cylinder 2, the upper end of the inner screen cylinder 3 is open, and the lower end of the inner screen cylinder is blocked.

The stirring subassembly is used for stirring broken to the placenta tissue in the interior screen drum 3, mainly comprises agitator motor 6, (mixing) shaft 7, agitator 8, agitator motor 6 is fixed in the lid 4 outside, and inside the agitator shaft 7 stretched into interior screen drum 3, its upper end stretched out the lid 4 outside and be connected with agitator motor 6's output, be equipped with a plurality of stirring vane on the agitator 8, be located agitator shaft 7's lower extreme, agitator 8 is equipped with two, is equipped with the rotatory first bearing 15 of support (mixing) shaft 7 between lid 4 and the agitator shaft 7. When the stirring motor 6 is started, the stirring shaft drives the stirrer 8 to rotate to stir and crush placenta tissues, so that placenta wall decidua and placenta chorion are separated.

The centrifugal component is used for driving the inner screen drum 3 to rotate, mainly composed of a centrifugal motor 9 and a centrifugal column 10, the base 1 is of a hollow structure, the centrifugal motor 9 is located inside the base 1, the output end of the centrifugal motor is connected with the centrifugal column 10, the upper end of the centrifugal column 10 extends out of the base 1, a rotation stopping block 11 with a triangular cross section is arranged at the upper end of the centrifugal column 10, a rotation stopping groove 12 with a shape matched with the rotation stopping block 11 is arranged at the outer side of the bottom of the inner screen drum 3 correspondingly, the centrifugal column 10 is in rotation stopping fit with the inner screen drum 3 through the rotation stopping block-rotation stopping groove, and in other embodiments, the cross section of the rotation stopping block can be in other non-circular shapes, for example: quadrangle, cross, etc. For supporting the stable rotation of the inner screen drum 3 relative to the outer cover drum 2, a rotary press ring 17 is rotatably mounted on the lower side of the cover body 4 through a second bearing 16, a limiting groove into which the upper end of the inner screen drum 3 extends is formed on the lower side of the rotary press ring 17, the groove bottom of the limiting groove and the groove wall of the limiting groove are used for limiting the vertical direction position and the radial position of the rotary press ring 17 respectively, the groove bottom of the limiting groove is in press fit with the upper end of the inner screen drum 3, and the rotary press ring and the inner screen drum 3 rotate together relative to the cover body 4 when the inner screen drum 3 rotates under the action of the centrifugal column 10.

In order to prevent the material from leaking into the base, a rotary sealing structure is arranged at the centrifugal column 10, a third bearing 18 for supporting the centrifugal column 10 to rotate and a base rotary sealing ring 19 for keeping the centrifugal column 10 and the base 1 in dynamic sealing fit are arranged between the centrifugal column 10 and the base 1; the centrifugal column 10 is also provided with a sealing convex ring 13 at the lower side of the rotation stopping block 11 and inside the outer cover cylinder 2, and the bottom of the sealing convex ring 13 is provided with a two-stage convex ring rotary sealing ring 14 for keeping the sealing convex ring 13 in rotary sealing fit with the base 1. The multistage sealing structure prevents materials from leaking and has good sealing effect. In other embodiments, the male ring rotary seal ring may be provided in other numbers of three, four, etc.

The tissue separation device of the utility model has the following use process: the outer cover cylinder is arranged on the base, the rotation stopping groove and the rotation stopping block are aligned, the inner screen cylinder is arranged, the cover body is arranged after placenta tissues are added into the inner screen cylinder, the stirring motor and the centrifugal motor are started, the stirring motor drives the stirring shaft and the stirrer to act, the placenta tissues are stirred and crushed, the centrifugal motor drives the centrifugal column and the inner screen cylinder to rotate, the crushed materials are subjected to centrifugal action, placenta wall decidua are thrown out through screen holes under the centrifugal action, and placenta chorion is retained inside the inner screen cylinder based on the thickness, so that the separation effect is achieved. In the utility model, the stirring and the centrifugation are respectively carried out under the action of the stirring motor and the centrifugation motor, so that the relative movement between the stirrer and the materials is ensured, the stirring action is complete and the separation effect is good; besides, the centrifugal assembly, the inner screen cylinder and the cover body are ingenious in structural design, the inner screen cylinder centrifugal rotation function is realized on the basis of the detachable inner screen cylinder, the outer cover cylinder and the base, the inner screen cylinder and the cover body and the inner screen cylinder and the base are in detachable connection, and all the components can be detached independently to clean, so that dead angle cleaning and troublesome cleaning are avoided.

The above is only a preferred embodiment of the present utility model, and the protection scope of the present utility model is not limited to the above examples, and all technical solutions belonging to the concept of the present utility model belong to the protection scope of the present utility model. It should be noted that modifications and adaptations to the present utility model may occur to one skilled in the art without departing from the principles of the present utility model and are intended to be within the scope of the present utility model.

Claims

1. The tissue separation device is characterized by comprising a base, an outer cover cylinder, an inner screen cylinder, a cover body, a stirring assembly and a centrifugal assembly, wherein the upper side of the base is provided with a mounting groove, the upper end and the lower end of the outer cover cylinder are both open, the lower end of the outer cover cylinder is arranged in the mounting groove in a threaded manner, the upper end of the inner screen cylinder is open and blocked, the inner screen cylinder is coaxially positioned in the outer cover cylinder, the outer side wall of the inner screen cylinder is provided with a plurality of screen holes, and the cover body is buckled on the upper side of the outer cover cylinder and is in threaded connection with the upper end of the outer cover cylinder;

2. A tissue separator according to claim 1, wherein the lower side of the rotary pressure ring is provided with a limit groove into which the upper end of the inner screen drum extends.

3. A tissue separation device as claimed in claim 1, wherein said agitator is provided with at least two.

4. A tissue separation device according to any one of claims 1-3, wherein the centrifugal column is in rotary sealing fit with the base through a third bearing and a base rotary sealing ring, a sealing convex ring is arranged on the centrifugal column at the lower side of the rotation stop block, and a convex ring rotary sealing ring for rotary sealing fit with the base is arranged at the lower side of the sealing convex ring.

5. A tissue separation device as claimed in claim 4, wherein the collar rotary seal is provided with at least two.