CN219326716U - Antibody purification device - Google Patents
Antibody purification device Download PDFInfo
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- CN219326716U CN219326716U CN202320508827.8U CN202320508827U CN219326716U CN 219326716 U CN219326716 U CN 219326716U CN 202320508827 U CN202320508827 U CN 202320508827U CN 219326716 U CN219326716 U CN 219326716U
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Abstract
The utility model relates to the technical field of antibody purification, and discloses an antibody purification device. According to the utility model, the purification column can be suspended by the two clamping plates and the two U-shaped grooves, the liquid inlet cover and the liquid outlet cover can be driven to be close to or far away from each other by the positioning mechanism, the liquid inlet cover and the liquid outlet cover can be connected with the liquid inlet of the purification column by the liquid inlet connecting mechanism, the liquid outlet connecting mechanism can be connected with the liquid outlet of the purification column, quantitative liquid can be introduced into the purification column by the liquid guide mechanism, the two-way screw can be driven to rotate by the servo motor, the two sliding plates can be driven to be close to or far away from each other by the two-way screw to position the purification column by the liquid inlet cover, and the liquid can be injected into the purification column by the liquid inlet pipe.
Description
Technical Field
The utility model relates to the technical field of antibody purification, in particular to an antibody purification device x.
Background
Antibodies are a special protein molecule used as reagents for in vitro diagnosis, agents for treating diseases, ligands for immunoaffinity chromatography, etc. Antibodies have wide application in the fields of life science research, biotechnology and medicine, and particularly as core reagents for various immunoassays, which play a vital role in the sensitivity and specificity of immunoassay results. The preparation of high-specificity and high-titer antibodies is the basis of immunological techniques, but the purification and separation techniques of antibodies need to be performed no matter what preparation technique is used, and currently include chromatographic column purification methods, ammonium sulfate precipitation methods and the like.
The existing antibody purification device adopting the chromatographic column purification method often needs to be manually installed and connected with a pipeline of the purification device before use, so that the existing antibody purification device often has the problem that the purification column is inconvenient to install and detach, and the purification column is inconvenient to replace.
Disclosure of Invention
The utility model provides an antibody purifying device, which aims to solve the technical problem that the existing antibody purifying device is inconvenient to replace a purifying column.
The utility model is realized by adopting the following technical scheme: an antibody purification device comprising: a bottom plate; the vertical plate is fixedly arranged at the top of the bottom plate; the top plate is fixedly arranged at the top of the vertical plate; the two limiting grooves are formed in the vertical plate; the two clamping plates are fixedly arranged on one side of the vertical plate; the two U-shaped grooves are respectively formed in the two clamping plates; the purification columns are arranged on the two U-shaped grooves; the sleeve plate is fixedly sleeved on the purification column; the positioning mechanism is arranged on the vertical plate; the liquid inlet connecting mechanism is arranged on the positioning mechanism; the liquid outlet connecting mechanism is arranged on the positioning mechanism; and the liquid guide mechanism is arranged at the top of the top plate.
Through above-mentioned technical scheme, can unsettled placing the purification post through two cardboard and two U type grooves, can drive feed liquor cover and flowing back cover and be close to each other or keep away from through positioning mechanism, can be connected with the inlet of purification post through feed liquor coupling mechanism, can be connected with the liquid outlet of purification post through play liquid coupling mechanism, can be with quantitative liquid introduction purification post through the drain mechanism in.
As the further improvement of above-mentioned scheme, positioning mechanism includes servo motor, two-way screw rod, a plurality of gag lever post and two sliding plates, servo motor fixed mounting is at the top of bottom plate, two-way screw rod fixed mounting is on servo motor's output shaft, two-way screw rod rotates with the roof to be connected, a plurality of gag lever posts are equal fixed mounting at the top of bottom plate, a plurality of gag lever posts all with roof fixed connection, two sliding plates all thread bush is established on two-way screw rod, two sliding plates all with a plurality of gag lever post sliding connection, two sliding plates respectively with the both sides inner wall sliding connection of two spacing grooves.
Through above-mentioned technical scheme, can drive two-way screw rod rotation through servo motor, can drive two sliding plates and be close to each other or keep away from through two-way screw rod rotation.
As a further improvement of the scheme, the liquid inlet connecting mechanism comprises a liquid inlet cover and a liquid inlet pipe, wherein the liquid inlet cover is fixedly arranged at the bottom of the sliding plate, and the liquid inlet pipe is arranged on the liquid inlet cover.
Through above-mentioned technical scheme, can fix a position the purification post through the feed liquor cover, can pour into the purification post with liquid through the feed liquor pipe in.
As a further improvement of the above scheme, the liquid outlet connection mechanism comprises a liquid outlet cover and a liquid outlet pipe, wherein the liquid outlet cover is fixedly arranged at the top of the sliding plate, the liquid outlet pipe is arranged on the liquid outlet cover, and one end of the liquid outlet pipe extends to the bottom of the sliding plate.
Through above-mentioned technical scheme, can fix a position the purification post through the flowing back cover, can discharge the liquid after the purification through the fluid-discharge tube.
As a further improvement of the scheme, the liquid guide mechanism comprises a measuring cup and a guide pipe, the measuring cup is arranged at the top of the top plate, the guide pipe is arranged at the bottom of the measuring cup, and the guide pipe is communicated with the liquid inlet pipe.
Through the technical scheme, liquid can be contained through the measuring cup.
As a further improvement of the scheme, the top of the measuring cup is provided with the cup cover, the top of the top plate is fixedly provided with the air pump, the air outlet of the air pump is provided with the pressurizing pipe, and the pressurizing pipe is connected with the cup cover.
Through above-mentioned technical scheme, can be with the inside of air injection graduated flask through air pump and pressurization pipe to promote the speed that liquid got into in the purification post, thereby promote purification rate.
As a further improvement of the scheme, scale marks are arranged on the measuring cup, and sealing rings are arranged on the inner walls of the liquid inlet cover and the liquid outlet cover.
Through above-mentioned technical scheme, can confirm the volume of injecting the interior liquid of graduated flask through the scale mark, can play sealed effect through two sealing washer to prevent the liquid seepage.
Compared with the prior art, the utility model has the beneficial effects that:
according to the utility model, the purification column can be suspended by the two clamping plates and the two U-shaped grooves, the liquid inlet cover and the liquid outlet cover can be driven to be close to or far away from each other by the positioning mechanism, the liquid inlet cover and the liquid outlet cover can be connected with the liquid inlet of the purification column by the liquid inlet connecting mechanism, the liquid outlet connecting mechanism can be connected with the liquid outlet of the purification column, quantitative liquid can be introduced into the purification column by the liquid guide mechanism, the two sliding plates can be driven to rotate by the servo motor, the two sliding plates can be driven to be close to or far away from each other by the two sliding plates by the rotation of the servo motor, the purification column can be positioned by the liquid inlet cover, the liquid can be injected into the purification column by the liquid inlet pipe, the purification column can be positioned by the liquid outlet cover, the purified liquid can be discharged by the liquid outlet pipe, the liquid can be contained by the measuring cup, the air can be injected into the measuring cup by the pressurizing pipe, so that the liquid enters the purification column, the purification speed can be improved, the volume of the liquid in the measuring cup can be determined by the scale marks, the sealing effect can be achieved, and the leakage of the liquid can be prevented.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of an antibody purification apparatus according to example 1 of the present utility model;
FIG. 2 is a schematic diagram of the front view of FIG. 1;
FIG. 3 is a schematic three-dimensional structure of the purification column of FIG. 2;
FIG. 4 is an enlarged schematic view of the structure of FIG. 1A;
fig. 5 is an enlarged schematic view of the structure at B in fig. 1.
Main symbol description:
1. a bottom plate; 2. a riser; 3. a top plate; 4. a limit groove; 5. a clamping plate; 6. a U-shaped groove; 7. a purification column; 8. a sleeve plate; 9. a servo motor; 10. a bidirectional screw; 11. a limit rod; 12. a sliding plate; 13. a liquid inlet cover; 14. a liquid inlet pipe; 15. a liquid discharge cover; 16. a liquid discharge pipe; 17. a measuring cup; 18. a conduit; 19. a cup cover; 20. an air pump; 21. a pressurized tube.
Detailed Description
The present utility model will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, the following embodiments or technical features may be arbitrarily combined to form new embodiments.
Examples:
referring to fig. 1 to 5, the antibody purifying apparatus of the present embodiment includes: a base plate 1; the vertical plate 2 is fixedly arranged at the top of the bottom plate 1; the top plate 3, the top plate 3 is fixedly arranged on the top of the vertical plate 2; the two limiting grooves 4 are formed in the vertical plate 2; the two clamping plates 5 are fixedly arranged on one side of the vertical plate 2; the two U-shaped grooves 6 are respectively formed in the two clamping plates 5; the purification columns 7, the purification columns 7 are arranged on the two U-shaped grooves 6; the sleeve plate 8 is fixedly sleeved on the purification column 7; the positioning mechanism is arranged on the vertical plate 2; the liquid inlet connecting mechanism is arranged on the positioning mechanism; the liquid outlet connecting mechanism is arranged on the positioning mechanism; and the liquid guide mechanism is arranged at the top of the top plate 3.
The positioning mechanism comprises a servo motor 9, a bidirectional screw rod 10, a plurality of limiting rods 11 and two sliding plates 12, wherein the servo motor 9 is fixedly arranged at the top of the bottom plate 1, the bidirectional screw rod 10 is fixedly arranged on an output shaft of the servo motor 9, the bidirectional screw rod 10 is rotationally connected with the top plate 3, the limiting rods 11 are fixedly arranged at the top of the bottom plate 1, the limiting rods 11 are fixedly connected with the top plate 3, the two sliding plates 12 are sleeved on the bidirectional screw rod 10 in a threaded manner, the two sliding plates 12 are slidably connected with the limiting rods 11, and the two sliding plates 12 are slidably connected with the inner walls of the two sides of the two limiting grooves 4 respectively.
The liquid inlet connecting mechanism comprises a liquid inlet cover 13 and a liquid inlet pipe 14, wherein the liquid inlet cover 13 is fixedly arranged at the bottom of the sliding plate 12, and the liquid inlet pipe 14 is arranged on the liquid inlet cover 13.
The liquid outlet connection mechanism comprises a liquid outlet cover 15 and a liquid outlet pipe 16, wherein the liquid outlet cover 15 is fixedly arranged at the top of the sliding plate 12, the liquid outlet pipe 16 is arranged on the liquid outlet cover 15, and one end of the liquid outlet pipe 16 extends to the bottom of the sliding plate 12.
The liquid guide mechanism comprises a measuring cup 17 and a conduit 18, wherein the measuring cup 17 is arranged at the top of the top plate 3, the conduit 18 is arranged at the bottom of the measuring cup 17, and the conduit 18 is communicated with the liquid inlet pipe 14.
The top of the measuring cup 17 is provided with a cup cover 19, the top of the top plate 3 is fixedly provided with an air pump 20, the air outlet of the air pump 20 is provided with a pressurizing pipe 21, and the pressurizing pipe 21 is connected with the cup cover 19.
Scale marks are arranged on the measuring cup 17, and sealing rings are arranged on the inner walls of the liquid inlet cover 13 and the liquid outlet cover 15.
The implementation principle of the antibody purification device in the embodiment of the application is as follows:
when the purification column 7 is used, the purification column 7 is placed in the two U-shaped grooves 6 on the two clamping plates 5, the sleeve plate 8 on the purification column 7 is placed between the two clamping plates 5, so that the purification column 7 is suspended between the liquid inlet cover 13 and the liquid outlet cover 15, the servo motor 9 is started, the servo motor 9 drives the bidirectional screw rod 10 to rotate, the bidirectional screw rod 10 rotates to drive the two sliding plates 12 to be close to each other, the liquid inlet cover 13 and the liquid outlet cover 15 are close to two ends of the purification column 7, the liquid inlet pipe 14 and the liquid outlet pipe 16 are respectively inserted into the liquid inlet and the liquid outlet of the purification column 7, and the sealing effect can be achieved through the two sealing rings, so that liquid leakage is prevented;
injecting quantitative liquid into the measuring cup 17 according to the scale marks on the observing measuring cup 17, then covering the cup cover 19, starting the air pump 20, injecting air into the measuring cup 17 by the air pump 20 through the pressurizing pipe 21, thereby increasing the air pressure in the measuring cup 17, leading the liquid in the measuring cup 17 to enter the purifying column 7 along the guide pipe 18 and the liquid inlet pipe 14, purifying antibodies in the liquid through the purifying column 7, discharging the liquid through the liquid discharge pipe 16, and placing a container at the bottom end of the liquid discharge pipe 16 by staff to receive the liquid;
after purification is completed, the servo motor 9 is controlled to reversely rotate, so that the liquid inlet cover 13 and the liquid outlet cover 15 are mutually far away, the liquid inlet pipe 14 and the liquid outlet pipe 16 are separated from the purification column 7, and then the purification column is taken down.
The above embodiments are only preferred embodiments of the present utility model, and the scope of the present utility model is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present utility model are intended to be within the scope of the present utility model as claimed.
Claims (7)
1. An antibody purification apparatus comprising:
a bottom plate;
the vertical plate is fixedly arranged at the top of the bottom plate;
the top plate is fixedly arranged at the top of the vertical plate;
the two limiting grooves are formed in the vertical plate;
the two clamping plates are fixedly arranged on one side of the vertical plate;
the two U-shaped grooves are respectively formed in the two clamping plates;
the purification columns are arranged on the two U-shaped grooves;
the sleeve plate is fixedly sleeved on the purification column;
the positioning mechanism is arranged on the vertical plate;
the liquid inlet connecting mechanism is arranged on the positioning mechanism;
the liquid outlet connecting mechanism is arranged on the positioning mechanism;
the liquid guide mechanism is arranged at the top of the top plate.
2. The antibody purification device of claim 1, wherein the positioning mechanism comprises a servo motor, a bidirectional screw, a plurality of limiting rods and two sliding plates, the servo motor is fixedly installed at the top of the bottom plate, the bidirectional screw is fixedly installed on an output shaft of the servo motor, the bidirectional screw is rotationally connected with the top plate, the limiting rods are fixedly installed at the top of the bottom plate, the limiting rods are fixedly connected with the top plate, the two sliding plates are sleeved on the bidirectional screw in a threaded manner, the two sliding plates are in sliding connection with the limiting rods, and the two sliding plates are respectively in sliding connection with two side inner walls of the limiting grooves.
3. The antibody purification apparatus of claim 2, wherein the fluid inlet connection mechanism comprises a fluid inlet housing fixedly mounted to the bottom of the slide plate and a fluid inlet tube disposed on the fluid inlet housing.
4. The antibody purification apparatus of claim 3, wherein the liquid outlet connection mechanism comprises a liquid outlet cover fixedly mounted on the top of the slide plate and a liquid outlet pipe provided on the liquid outlet cover, one end of the liquid outlet pipe extending to the bottom of the slide plate.
5. The antibody purification apparatus of claim 4, wherein the liquid guiding mechanism comprises a measuring cup and a conduit, the measuring cup is disposed at the top of the top plate, the conduit is disposed at the bottom of the measuring cup, and the conduit is in communication with the liquid inlet tube.
6. The antibody purification apparatus of claim 5, wherein a cap is provided on top of the measuring cup, an air pump is fixedly installed on top of the top plate, a pressurizing tube is provided on an air outlet of the air pump, and the pressurizing tube is connected with the cap.
7. The antibody purification apparatus of claim 6, wherein the graduated scale is provided on the measuring cup, and sealing rings are provided on the inner walls of the liquid inlet cover and the liquid outlet cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320508827.8U CN219326716U (en) | 2023-03-16 | 2023-03-16 | Antibody purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320508827.8U CN219326716U (en) | 2023-03-16 | 2023-03-16 | Antibody purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219326716U true CN219326716U (en) | 2023-07-11 |
Family
ID=87066240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320508827.8U Active CN219326716U (en) | 2023-03-16 | 2023-03-16 | Antibody purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219326716U (en) |
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2023
- 2023-03-16 CN CN202320508827.8U patent/CN219326716U/en active Active
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