CN219279845U

CN219279845U - Film transfer device for biochemical experiment

Info

Publication number: CN219279845U
Application number: CN202320095944.6U
Authority: CN
Inventors: 马文豪; 陆晨; 董哲岳
Original assignee: Beijing Jinlan Gene Technology Co ltd
Current assignee: Beijing Jinlan Gene Technology Co ltd
Priority date: 2023-02-01
Filing date: 2023-02-01
Publication date: 2023-06-30
Anticipated expiration: 2033-02-01

Abstract

The utility model relates to a film transfer device for biochemical experiments. The film transferring device comprises a base (1) for holding a film transferring box (3), a support (2) which is vertically connected with the base (1) and is arranged on one side of the base (1), and a pressing device which is arranged above the base (1), wherein a sliding rail is arranged on the support (2), the pressing device is fixedly connected with a sliding block, and the sliding block can slide along the sliding rail so as to adjust the height of the pressing device. According to the utility model, the slide rail arranged on the bracket is in sliding fit with the slide block fixedly connected with the pressing device, so that the pressing plate can be adjusted in height while being kept horizontal, and the operation of adjusting the pressing plate is simplified.

Description

Film transfer device for biochemical experiment

Technical Field

The utility model relates to biochemical experiment equipment, in particular to a film transfer device for biochemical experiments.

Background

In biochemical and molecular biological scientific research, in order to verify the copy number of genes and the transcription of genes, it is often necessary to perform a molecular hybridization experiment of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid), one of the important links is to perform a blotting and transfer experiment of nucleic acids. There are three general methods of transfer: capillary blotting transfer method, vacuum transfer method and electrotransfer blotting method, wherein the capillary blotting transfer method is widely applied due to simple operation, low cost and high transfer efficiency.

When a capillary blotting transfer method is used for performing a nucleic acid blotting transfer experiment, such as a Southern blot experiment, a container capable of containing transfer film solution is generally adopted as a transfer film box, an object carrying platform is arranged at the upper part of the transfer film box, a pressing plate with a counterweight capable of moving up and down is arranged above the object carrying platform, and the pressing plate is connected with the transfer film box through a sliding rod.

For example, chinese patent publication CN205774458U discloses a nucleic acid blotting membrane transfer device for nucleic acid molecule hybridization experiments, which comprises a bottom plate, a membrane transfer box arranged in the middle of the bottom plate, membrane transfer columns arranged at four corners of the bottom plate, and a pressing plate arranged above the membrane transfer box, wherein the pressing plate is provided with a through hole matched with the membrane transfer column, and the membrane transfer column is inserted into the through hole and longitudinally slidably connected with the pressing plate. Because four corners of the pressing plate are limited by the rotating film column, the inclination in the rotating film process is avoided, and side turning is not easy to occur. In addition, the pressing plate is slidably connected with the rotating film column, so that the height of the pressing plate can be adjusted, and different rotating film boxes can be adapted.

However, the film transfer device disclosed in the above patent document needs to adjust the height of the pressing plate through the longitudinal sliding of the four through holes and the four film transfer columns of the pressing plate every time when in use, and is complex in operation. Moreover, a level gauge is required above the platen to monitor whether the platen is level after each height adjustment (i.e., whether the platen needs to be readjusted), which is time consuming overall operation. In addition, the weight of the transfer film device is 500g, the weight is not adjustable, and when the transfer area is small, the weight may crush the transfer film.

Disclosure of Invention

The present utility model has been made in view of the above background, and aims to provide a transfer film device for biochemical experiments capable of allowing a platen to be highly adjusted while maintaining a horizontal state.

The utility model particularly provides a film transferring device for biochemical experiments, which comprises a base for holding a film transferring box, a support vertically connected with the base on one side of the base and a pressing device arranged above the base, wherein a sliding rail is arranged on the support, the pressing device is fixedly connected with a sliding block, and the sliding block can slide along the sliding rail so as to adjust the height of the pressing device.

In one embodiment of the utility model, the hold down device includes a hold down plate and a weight slot disposed above the hold down plate.

In one embodiment of the utility model, the weight of the weight arranged in the weight groove is adjustable.

In one embodiment of the utility model, the weight is a lead bead.

In one embodiment of the utility model, the pressure plate is integrally formed with the weight slot.

In one embodiment of the utility model, a plurality of through holes are arranged on the pressing plate or the weight groove, and bolts can pass through the through holes and corresponding through holes on the sliding block and are fastened by nuts, so that the pressing device and the sliding block are fixedly connected together.

In one embodiment of the utility model, the slider comprises a first portion and a second portion perpendicular to each other, the first portion being able to slide along the sliding rail, the second portion being provided with the corresponding through hole.

In one embodiment of the utility model, the base is integrally formed with the bracket.

In one embodiment of the utility model, the bellows comprises a groove extending through the base from front to back, the base comprising a horizontal portion extending through the groove and two vertical portions clamping the bellows from front to back.

In one embodiment of the utility model, the film transfer device further comprises a carrying platform arranged above the film transfer box, and the carrying platform is fixedly arranged on the base.

According to the utility model, the slide rail arranged on the bracket is in sliding fit with the slide block fixedly connected with the pressing device, so that the pressing plate can be adjusted in height while being kept horizontal, and the operation of adjusting the pressing plate is simplified.

Drawings

The above and other aspects, features and benefits of various embodiments of the present utility model will become more apparent from the following detailed description with reference to the accompanying drawings. The same reference numbers or numbers will be used throughout the drawings to refer to the same or like elements. The drawings are not to the same scale, wherein:

FIG. 1 is a schematic view showing a transfer device for biochemical experiments according to an embodiment of the present utility model, in which a slide rail and a slider are omitted;

fig. 2A and 2B are front and side views of a base-bracket assembly of the film transfer device in this embodiment;

fig. 3A and 3B are front and side views of a platen-weight slot assembly of the film transfer apparatus in this embodiment;

fig. 4 is a front view of the bellows in this embodiment.

Detailed Description

Exemplary embodiments of the present utility model will be described below with reference to the accompanying drawings. It should be noted that the described embodiments are merely examples of the present utility model and are not intended to limit the present utility model. All other embodiments, which can be obtained by a person skilled in the art on the basis of the described embodiments without the need for inventive labour, fall within the scope of the utility model.

Fig. 1 shows a transfer device for biochemical experiments according to an embodiment of the present utility model, in which slide rails and sliders are omitted. Fig. 2A and 2B are front and side views of a base-bracket assembly of the film transfer device in this embodiment. Fig. 3A and 3B are front and side views of a platen-weight slot assembly of the film transfer apparatus in this embodiment. Fig. 4 is a front view of the bellows in this embodiment.

As shown in fig. 1 to 4, the film transfer device in this embodiment includes a base 1, a bracket 2, a film transfer box 3, a carrying platform 4, a pressing plate 5, a slide rail 6, a slider 7, and a weight slot 8.

The base 1, the support 2, the bellows 3, the load platform 4, the pressure plate 5 and the weight trough 8 may be made of resin or plastic material. The slide rail 6 and the slider 7 may be made of a metal material. The slide rail 6 is arranged on the bracket 2, and the slide block 7, the pressing plate 5 and the weight groove 8 are fixed together. Fig. 1 shows only a portion of the film transfer device made of resin or plastic, omitting the slide rail 6 and the slider 7 made of a metal material.

The base 1 is used for holding a transfer membrane box 3 for containing transfer membrane solution. The bracket 2 is fixedly connected with the base 1 at the rear side of the base 1 to form a base-bracket assembly. The base 1 is arranged generally horizontally and the stand 2 is arranged generally vertically. That is, the bracket 2 is vertically connected to the base 1. In this embodiment, the base 1 and the bracket 2 are integrally formed by using a known process such as 3D printing or injection molding. In other embodiments, the base 1 and the bracket 2 may be formed separately and then fixed together by welding, screwing, or the like.

The bellows 3 in this embodiment is substantially rectangular parallelepiped box-shaped and includes a groove 10 penetrating front and rear as shown in fig. 4. Accordingly, the base 1 includes a horizontal portion which can pass through the groove 10 and two vertical portions which can clamp the bellows 3 from both front and rear sides, the horizontal portion being higher than the vertical portions, as shown in fig. 1. The bracket 2 is integrated with a rear vertical portion of the base 1.

The carrying platform 4 is arranged above the transfer box 3 and is used for placing gel, a cellulose acetate film (NC film) or a nitrocellulose film, absorbent paper and the like. In this embodiment, the carrying platform 4 is fixedly mounted on the base 1. For example, the front and rear ends of the horizontal portion of the base 1 may be higher and wider than the middle portion on which the loading platform 4 is placed and carried by, and the lower surface of the loading platform 4 may be provided with two protrusions, and the front and rear ends of the horizontal portion of the base 1 restrict the movement of the loading platform 4 in the front and rear directions, and the two protrusions on the lower surface of the loading platform 4 contact the middle portion of the horizontal portion of the base 1 to restrict the movement of the loading platform 4 in the left and right directions. Instead of the convex strips provided on the lower surface of the carrying platform 4, convex portions protruding forward or backward may be provided at the middle portions of the front and rear side edges of the carrying platform 4, and corresponding engaging grooves may be provided at the front and rear end portions of the horizontal portion of the base 1. In other embodiments, the carrying platform 4 may be integrally formed with the base 1 or welded together. In other embodiments, the loading platform 4 may also be fixedly mounted on the bellows 3.

The platen 5 and the weight slot 8 disposed thereabove may be integrally formed using known processes such as 3D printing or injection molding to form a platen-weight slot assembly, i.e., a hold down device. In this embodiment, the weight provided in the weight groove 8 is a lead ball, and the weight mass can be adjusted by changing the number of lead balls. Of course, other weights than lead beads may be used.

The slide rail 6 is provided on the bracket 2 and extends in the vertical direction as shown in fig. 2A and 2B. For example, the slide rail 6 may be fixed to the front side surface of the bracket 2 by bolts.

The slide 7 is arranged on the platen-weight slot assembly. For example, as shown in fig. 3A and 3B, the weight slot 8 may be provided with a plurality of through holes 9 (four holes are shown in the drawings), and the slider 7 may be provided with corresponding through holes through which bolts may be passed in the horizontal direction and then fastened with nuts, thereby fixedly connecting the platen-weight slot assembly with the slider 7. The slide 7 may comprise a first portion and a second portion perpendicular to each other, wherein the first portion is capable of sliding along the slide rail 6 and the second portion may abut against the rear surface of the weight slot 8 and be provided with said through hole.

In the embodiment shown in fig. 3A and 3B, the first and second parts of the slider 7 are arranged in two vertical planes perpendicular to each other. In a further embodiment, the second portion of the slider 7 may extend along a horizontal plane at the bottom of the first portion. Accordingly, a plurality of through holes may be provided at corresponding positions on the second portion of the slider 7 and the rear portion of the pressing plate 5, and bolts may be passed through the through holes in the vertical direction and then fastened with nuts, thereby fixedly coupling the pressing plate-weight groove assembly with the slider 7.

In use of the transfer device of the illustrated embodiment, transfer box 3 is first placed on a table and a transfer solution is injected, and then a base-support assembly is provided such that the horizontal portion of base 1 is positioned in recess 10 of transfer box 3, and transfer box 3 is held from above and from both front and rear sides by the horizontal portion and two vertical portions of base 1. Then, the loading platform 4 is fixed on the base 1, and a gel, NC film or nitrocellulose film, water absorbing paper, or the like is placed on the loading platform 4. By sliding the slide 7 longitudinally along the slide rail 6, the platen-weight slot assembly can be placed at an appropriate height to compress the gel or the like from above. When the absorbent paper and the like need to be replaced, the sliding block 7 only needs to slide up and down along the sliding rail 6.

In the film transfer device provided by the utility model, the bracket 2 is vertically connected with the base 1, and the height of the pressing plate 5 is adjusted through sliding fit between the sliding rail 6 arranged on the bracket 2 and the sliding block 7 fixedly connected with the pressing device comprising the pressing plate 5. Therefore, the height adjustment can be performed while the platen is kept horizontal, simplifying the operation of adjusting the platen.

In the present utility model, the weight mass (for example, the number of lead beads in the weight tank 8 is changed) can be adjusted according to the experimental requirements, so that the transfer film can be prevented from being crushed due to the excessive weight mass.

The transfer device provided by the utility model is particularly suitable for Southern blot experiments. However, the utility model is not limited thereto, and can be applied to any other experimental situation requiring blotting transfer.

It is to be understood that the above embodiments are merely illustrative of the application of the principles of the present utility model, but not in limitation thereof. It will be apparent to those skilled in the art that various modifications and improvements can be made to the present utility model without departing from the spirit or essential scope of the utility model.

Claims

1. The film transfer device for the biochemical experiment is characterized by comprising a base (1) for holding a film transfer box (3), a support (2) vertically connected with the base (1) on one side of the base (1) and a pressing device arranged above the base (1), wherein a sliding rail (6) is arranged on the support (2), the pressing device is fixedly connected with a sliding block (7), and the sliding block (7) can slide along the sliding rail (6) so as to adjust the height of the pressing device.

2. The transfer device for biochemical experiments according to claim 1, characterized in that the compression device comprises a pressure plate (5) and a weight trough (8) arranged above the pressure plate (5).

3. The transfer device for biochemical experiments according to claim 2, characterized in that the weight of the weight arranged in the weight groove (8) is adjustable.

4. A transfer device for biochemical experiments according to claim 3, wherein the weight is a lead bead.

5. The transfer membrane device for biochemical experiments according to claim 2, characterized in that the pressure plate (5) is integrally formed with the weight groove (8).

6. The membrane transfer device for biochemical experiments according to claim 5, wherein a plurality of through holes (9) are formed in the pressing plate (5) or the weight groove (8), and bolts can pass through the through holes and corresponding through holes in the sliding block (7) and are fastened by nuts, so that the pressing device is fixedly connected with the sliding block (7).

7. The transfer device for biochemical experiments according to claim 6, characterized in that the slide (7) comprises a first portion and a second portion perpendicular to each other, the first portion being able to slide along the slide rail (6), the second portion being provided with the corresponding through hole.

8. The transfer device for biochemical experiments according to any one of claims 1 to 7 wherein the base (1) is integrally formed with the support (2).

9. The transfer membrane device for biochemical experiments according to any one of claims 1 to 7 wherein the transfer membrane cartridge (3) comprises a groove (10) penetrating front and rear, the base (1) comprising a horizontal portion passing through the groove (10) and two vertical portions clamping the transfer membrane cartridge (3) from front and rear sides.

10. The transfer device for biochemical experiments according to any one of claims 1 to 7 further comprising a carrying platform (4) arranged above the transfer box (3), the carrying platform (4) being fixedly mounted on the base (1).