CN219278296U

CN219278296U - DNA extraction equipment

Info

Publication number: CN219278296U
Application number: CN202223504735.7U
Authority: CN
Inventors: 吴浪
Original assignee: Shanghai Permed Biomedical Co ltd
Current assignee: Shanghai Permed Biomedical Co ltd
Priority date: 2022-12-27
Filing date: 2022-12-27
Publication date: 2023-06-30
Anticipated expiration: 2032-12-27

Abstract

The utility model provides DNA extraction equipment which comprises an outer tube, wherein an inner thread is formed in the inner wall of the outer tube, an inner tube is movably installed in the outer tube through the inner thread, a sealing mechanism is fixedly installed in the inner tube, the sealing mechanism comprises a rubber disc, and an installation groove is formed in the inner portion of the bottom end of the inner tube. According to the utility model, the knob drives the rotating rod to rotate, and the rotating rod drives the rubber disc, so that the adjusting holes on the rubber disc are aligned with the through holes, the solution in the outer tube is conveniently discharged into the inner tube, the inner tube is sealed by the sealing mechanism, and the solution is conveniently stored and transferred.

Description

DNA extraction equipment

Technical Field

The utility model relates to the field of DNA extraction equipment, in particular to DNA extraction equipment.

Background

DNA carries genetic information necessary for synthesizing RNA and protein, is a biological macromolecule essential for the development and normal operation of organisms, and consists of a macromolecular polymer of deoxynucleotides. The extraction modes of DNA generally include: phenol extraction, formamide depolymerization, glass rod winding, heat centrifugation, etc. In the process of extracting DNA by phenol extraction, firstly, adding phenol to denature proteins in the cell tissue fluid so as to separate the proteins from the DNA, then adding chloroform to uniformly mix and centrifuge, separating the DNA from other impurities, and the DNA exists in supernatant. In this process of extracting DNA, a DNA extraction apparatus is used.

The Chinese patent of publication No. CN211005374U proposes a plasmid DNA extraction equipment, including the storage bottle, the middle part fixed mounting of storage bottle inner chamber has first rubber seal cover and second rubber seal cover, the second rubber seal cover is located the below of first rubber seal cover, the inner wall swing joint of first rubber seal cover and second rubber seal cover has the extraction tube, the storage storehouse has been seted up to the bottom of storage bottle, the bottom swing joint of storage bottle has the external member, the outer wall fixedly connected with fixed block of external member, the bottom fixed mounting of external member inner wall has the sealing plug, the through-hole has been seted up to the bottom of storage bottle, the sealing plug looks adaptation with the through-hole of storage bottle bottom. In this technical scheme, the bottom of storing the bottle is the pointed end, can not directly prevent on the desktop to first rubber seal cover sets up in the inside of storing the bottle, and it falls ash etc. easily on it, brings the inside of DNA together with the dust after inserting first rubber ground rice cover with the extraction tube, produces the influence to the DNA.

Disclosure of Invention

In order to solve the above-described problems, the present application provides a DNA extraction apparatus.

The application provides a DNA extraction equipment adopts following technical scheme: the utility model provides a DNA draws equipment, includes the outer tube, the internal thread has been seted up on the inner wall of outer tube, the inside of outer tube is through internal thread movable mounting inner tube, the inside fixed mounting of inner tube has closing mechanism, closing mechanism includes the rubber dish, the mounting groove has been seted up to the inside of the bottom of inner tube, the rubber dish rotates the inside of installing at the mounting groove, the regulation hole has been seted up to the inside symmetry of rubber dish, the bottom fixed mounting of inner tube has the rubber circle, the outside of inner tube is provided with the external screw thread.

Through the technical scheme, the sealing mechanism inside the inner tube can seal the two ends of the inner tube, so that the inner tube can store and transfer DNA solution, and DNA can be conveniently extracted.

Further, a rotating rod is fixedly installed at the center of the top of the rubber disc, the rotating rod is rotatably installed inside the inner tube, and through holes are symmetrically formed in the top and the bottom of the installation groove.

Through above-mentioned technical scheme, the rotation of dwang can drive the rubber dish and rotate, conveniently will adjust hole and through-hole alignment to the inside of the solution entering inner tube of outer tube of being convenient for.

Further, a torsion spring is fixedly arranged between the top end of the rotating rod and the inner tube, a knob is fixedly arranged at the top of the rotating rod, and the knob is movably arranged at the top of the inner tube.

Through above-mentioned technical scheme, can rotate the dwang through the knob, the dwang also can reverse rotation under the torsion of torsional spring for the dwang resets.

Further, a T-shaped hole is formed in the top end of the rotating rod, and two ends of the T-shaped hole are arranged on two sides of the rotating rod.

Through above-mentioned technical scheme, can be with the inside air discharge of inner tube through T type hole, conveniently get into the inside with the inside solution of outer tube.

Further, the outside of the top of dwang corresponds the bottom spiro union in T type hole and has sealing disc, the inboard top bilateral symmetry fixed mounting of inner tube has the dead lever, sealing disc slidable mounting is on the dead lever.

Through above-mentioned technical scheme, can drive the sealing disk and slide on the dead lever when the dwang is rotatory, be convenient for seal the both ends of the bottom of T type hole through the sealing disk.

Further, an air hole is formed in the center of the knob, and a rubber plug is fixedly installed at the top of the air hole through an anti-lost wire.

Through above-mentioned technical scheme, installation rubber buffer at the top of gas pocket can prevent that dust etc. from getting into the inside in gas pocket and T type hole, avoids causing gas pocket and T type hole to block up, is convenient for exhaust.

Further, the outer side of the top end of the outer tube is fixedly provided with an outer tube rubber sleeve, and the outer side of the top end of the inner tube is fixedly provided with an inner tube rubber sleeve at equal intervals.

Through above-mentioned technical scheme, can increase through outer tube rubber sleeve and inner tube rubber sleeve and add the frictional force of holding equipment and hand and outer tube, the inner tube between, conveniently add outer tube and inner tube and hold.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the outer tube and the inner tube are in threaded connection through the external threads and the internal threads, the outer tube and the inner tube can be installed and detached, cell sap is conveniently placed in the outer tube, the device can be placed in the centrifugal mechanism through the combination of the outer tube and the inner tube for centrifugal operation, and the outer tube and the inner tube are conveniently clamped through the outer tube rubber sleeve and the inner tube rubber sleeve.

2. After centrifugation, through rotating the knob, the knob drives dwang and rubber dish for regulation hole on the rubber dish aligns with the through-hole, opens the rubber buffer, rotates the inner tube, and the dwang drives the sealing disk simultaneously and upwards slides, and the sealing disk separates with the bottom in T type hole, and inner tube and external intercommunication, the supernatant passes through the inside that through-hole, regulation hole got into the inner tube, conveniently separates DNA and deposit.

3. Reverse rotation knob or loosen the knob, knob and dwang reverse rotation under the torsion of torsional spring to drive the rubber dish, make regulation hole and the through-hole dislocation on the rubber dish, thereby seal the bottom of inner tube, the dwang drives the sealing disc simultaneously and moves down, seals the bottom in T type hole, conveniently seals the save to DNA through the inner tube, is convenient for shift it.

Drawings

FIG. 1 is a schematic perspective view of the present application;

FIG. 2 is a schematic cross-sectional view of the present application;

FIG. 3 is a schematic view of the structure of FIG. 2A of the present application;

FIG. 4 is a schematic view of the structure of FIG. 2B of the present application;

FIG. 5 is a schematic view of the rubber disc structure of the present application;

fig. 6 is a schematic diagram of the structure of the antenna rotating rod;

in the figure: 1. an outer tube; 2. an inner tube; 3. an outer tube rubber sleeve; 4. a knob; 5. an inner tube rubber sleeve; 6. an external thread; 7. a rubber stopper; 8. a rotating lever; 9. a mounting groove; 10. a rubber ring; 11. a rubber plate; 12. a through hole; 13. a sealing plate; 14. a fixed rod; 15. an adjustment aperture; 16. t-shaped holes.

Detailed Description

The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.

Referring to fig. 1 to 6, the present utility model provides a technical solution: the utility model provides a DNA draws equipment, including outer tube 1, the internal thread has been seted up on the inner wall of outer tube 1, the inside of outer tube 1 is through internal thread movable mounting inner tube 2, the inside fixed mounting of inner tube 2 has closing mechanism, closing mechanism includes rubber dish 11, mounting groove 9 has been seted up to the inside of the bottom of inner tube 2, the inside at mounting groove 9 is installed in the rotation of rubber dish 11, regulation hole 15 has been seted up to the inside symmetry of rubber dish 11, the bottom fixed mounting of inner tube 2 has rubber circle 10, the outside of inner tube 2 is provided with external screw thread 6, through the regulation to closing mechanism such as rubber dish 11, can seal the both ends of inner tube 2, thereby make inner tube 2 become sealed container, conveniently deposit and shift the solution.

Referring to fig. 2, fig. 3, fig. 4 and fig. 5, the central point of the top of rubber disk 11 goes out fixed mounting and has dwang 8, dwang 8 rotates the inside of installing at inner tube 2, through-hole 12 has been seted up to the top and the bottom symmetry of mounting groove 9, fixed mounting has the torsional spring between the top of dwang 8 and inner tube 2, the top fixed mounting of dwang 8 has knob 4, knob 4 activity sets up the top at inner tube 2, T type hole 16 has been seted up to the inside at the top of dwang 8, the both ends setting of T type hole 16 are in the both sides of dwang 8, the outside at the top of dwang 8 corresponds the bottom spiro union of T type hole 16 has sealing disk 13, the inboard top bilateral symmetry fixed mounting of inner tube 2 has dead lever 14, sealing disk 13 slidable mounting is on dead lever 14, the central point department of knob 4 has seted up the gas pocket, the top of gas pocket is through anti-lost line fixed mounting rubber plug 7, knob 4 drives dwang 8, dwang 8 drives rubber disk 11 and rotates in the inside of mounting groove 9 for adjust hole 15 and through-hole 12 are aligned from top to bottom to inside of inner tube 2, the solution of the inside of nearly 1.

Referring to fig. 1 and 2, an outer pipe rubber sleeve 3 is fixedly installed on the outer side of the top end of the outer pipe 1, an inner pipe rubber sleeve 5 is fixedly installed on the outer side of the top end of the inner pipe 2 at equal intervals, friction between a holding device and a hand and the outer pipe 1 and the inner pipe 2 is increased, and the outer pipe 1 and the inner pipe 2 are conveniently held.

The implementation principle of the DNA extraction equipment in the embodiment of the application is as follows: the cell solution is put into the inside of the outer tube 1, the corresponding reagent is added, the inner tube 2 is screwed up the inside of the outer tube 1 through the external thread 6 and the internal thread, the device is put into the inside of the centrifugal device for centrifugal operation, after centrifugal completion, the knob 4 is rotated, the knob 4 drives the rotating rod 8 and the torsion spring to rotate, torsion is generated by the torsion spring, the top end of the rotating rod 8 drives the sealing disc 13, the sealing disc 13 slides downwards at the limit of the fixed rod 14, the sealing disc 13 leaves the bottom end of the T-shaped hole 16, the inner tube 2 is communicated with the outside through the T-shaped hole 16, the rotating rod 8 drives the rubber disc 11 to rotate in the inside of the mounting groove 9, at the moment, the adjusting hole 15 is aligned with the through hole 12 up and down, the central shaft is on the same straight line, the inner tube 2 is rotated through the inner tube rubber sleeve 5, the inner tube 2 is rotated towards the inside of the outer tube 1, the supernatant enters the inside of the inner tube 2 through the adjusting hole 15 and the through hole 12, the rotating knob 4 reversely rotates, the rotating rod 8 drives the rubber disc 11 to rotate through the through hole 12, the through hole 15 and the sealing disc 15 is misplaced, the bottom end of the inner tube 2 is driven by the rotating rod 8, the sealing disc 13 is communicated with the T-shaped hole 16, and the inner tube is simultaneously, the inner tube is driven by the rotating rod 8, the T-shaped sealing disc is subjected to transfer the T-shaped hole, and the supernatant is stored.

The advantages of this embodiment are: the pipe and the inner pipe are in threaded connection through the external thread and the internal thread, the outer pipe and the inner pipe can be installed and detached, cell sap is conveniently placed in the outer pipe, the equipment can be placed in the centrifugal mechanism through the combination of the outer pipe and the inner pipe for centrifugal operation, and the outer pipe and the inner pipe are conveniently clamped through the outer pipe rubber sleeve and the inner pipe rubber sleeve.

After centrifugation, through rotating the knob, the knob drives dwang and rubber dish for regulation hole on the rubber dish aligns with the through-hole, opens the rubber buffer, rotates the inner tube, and the dwang drives the sealing disk simultaneously and upwards slides, and the sealing disk separates with the bottom in T type hole, and inner tube and external intercommunication, the supernatant passes through the inside that through-hole, regulation hole got into the inner tube, conveniently separates DNA and other deposits.

Reverse rotation knob or loosen the knob, knob and dwang reverse rotation under the torsion of torsional spring to drive the rubber dish, make regulation hole and the through-hole dislocation on the rubber dish, thereby seal the bottom of inner tube, the dwang drives the sealing disc simultaneously and moves down, seals the bottom in T type hole, conveniently seals the save to DNA through the inner tube, is convenient for shift it.

While the fundamental and principal features of the utility model and advantages of the utility model have been shown and described, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims

1. A DNA extraction apparatus comprising an outer tube (1), characterized in that: the inner wall of outer tube (1) has seted up the internal thread, the inside of outer tube (1) is through internal thread movable mounting inner tube (2), the inside fixed mounting of inner tube (2) has closing mechanism, closing mechanism includes rubber dish (11), mounting groove (9) have been seted up to the inside of the bottom of inner tube (2), rubber dish (11) rotate and install the inside at mounting groove (9), regulation hole (15) have been seted up to the inside symmetry of rubber dish (11), the bottom fixed mounting of inner tube (2) has rubber circle (10), the outside of inner tube (2) is provided with external screw thread (6).

2. A DNA extraction apparatus according to claim 1, characterized in that: the center position of the top of the rubber disc (11) is fixedly provided with a rotating rod (8), the rotating rod (8) is rotatably arranged in the inner tube (2), and the top and the bottom of the mounting groove (9) are symmetrically provided with through holes (12).

3. A DNA extraction apparatus according to claim 2, characterized in that: a torsion spring is fixedly arranged between the top end of the rotating rod (8) and the inner tube (2), a knob (4) is fixedly arranged at the top of the rotating rod (8), and the knob (4) is movably arranged at the top of the inner tube (2).

4. A DNA extraction apparatus according to claim 3, characterized in that: t-shaped holes (16) are formed in the top end of the rotating rod (8), and two ends of each T-shaped hole (16) are arranged on two sides of the rotating rod (8).

5. The DNA extraction apparatus according to claim 4, wherein: the sealing device is characterized in that the outer side of the top end of the rotating rod (8) is in threaded connection with a sealing disc (13) corresponding to the bottom of the T-shaped hole (16), fixing rods (14) are symmetrically and fixedly arranged on the two sides of the inner side of the inner tube (2), and the sealing disc (13) is slidably arranged on the fixing rods (14).

6. A DNA extraction apparatus according to claim 3, characterized in that: an air hole is formed in the center of the knob (4), and a rubber plug (7) is fixedly arranged at the top of the air hole through an anti-lost wire.

7. The DNA extraction apparatus of claim 6, wherein: the outer side of the top end of the outer tube (1) is fixedly provided with an outer tube rubber sleeve (3), and the outer side of the top end of the inner tube (2) is fixedly provided with an inner tube rubber sleeve (5) at equal intervals.