CN219278147U

CN219278147U - Whole blood nucleic acid extraction kit based on nanometer magnetic beads

Info

Publication number: CN219278147U
Application number: CN202223156998.3U
Authority: CN
Inventors: 黄喜洛; 肖宝; 张培蕾
Original assignee: Henan Jerry Biotechnology Research Institute Co ltd
Current assignee: Henan Jerry Biotechnology Research Institute Co ltd
Priority date: 2022-11-28
Filing date: 2022-11-28
Publication date: 2023-06-30
Anticipated expiration: 2032-11-28

Abstract

The utility model discloses a whole blood nucleic acid extraction kit based on nanometer magnetic beads, which comprises an outer box, wherein a cavity is formed in one side of the outer box, a kit is fixedly arranged in the cavity in a sliding manner, a knob is rotatably arranged on the other side of the outer box, a threaded rod is fixedly arranged at one end of the knob, a threaded hole matched with the threaded rod is formed below the kit, the threaded rod penetrates through the outer box and is in threaded connection with the threaded hole, the cavity is formed in one side of the outer box, the kit is slidably arranged in the cavity, the threaded rod penetrates through the outer box and is in threaded connection with the threaded hole formed below the kit, one end of the threaded rod is fixedly arranged on the knob on the other side of the kit, and the threaded rod is driven to rotate by rotation of the knob, so that the kit can be pushed out or retracted into the outer box.

Description

Whole blood nucleic acid extraction kit based on nanometer magnetic beads

Technical Field

The utility model relates to the technical field of nucleic acid extraction kits, in particular to a whole blood nucleic acid extraction kit based on nano magnetic beads.

Background

The whole blood nucleic acid extraction kit based on the nano magnetic beads is prepared into the superparamagnetic silica nano magnetic beads after the surface of the superparamagnetic nano particles is modified and surface-modified by a nano technology, the magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface, and DNA can be separated from a blood sample under the action of chaotropic salt and an externally applied magnetic field by utilizing the superparamagnetism of the silica nano microspheres.

At present, when a whole blood nucleic acid extraction test tube for extracting the whole blood nucleic acid of the nano magnetic beads is fixed by the whole blood nucleic acid extraction kit on the market, the whole blood nucleic acid extraction kit has no better fixing structure, and the test tube is easy to skew and deviate in the process of taking out the kit and withdrawing the kit, so that the nucleic acid separation position is deviated, and the nucleic acid extraction effect is influenced.

The patent of publication No. CN214654819U discloses a whole blood nucleic acid extraction kit based on nanometer magnetic beads, which comprises a reagent box, one side of kit is rotated through the scarf joint bearing and is installed the worm, and the bottom inner wall of kit rotates and is connected with two pivots, one side of pivot fixedly connected with worm wheel and gear respectively, and the worm wheel meshes with the worm, the both sides sliding connection of kit has a smooth section of thick bamboo, one side fixedly connected with rack of smooth section of thick bamboo, rack meshes with the gear, one side inner wall fixedly connected with spring of smooth section of thick bamboo, one end fixedly connected with stopper of spring, one side fixedly connected with connecting rod of stopper, one end fixedly connected with clamp splice of connecting rod, and connecting rod and smooth section of thick bamboo sliding connection, the inside of kit is equipped with the standing groove, and the bottom fixedly connected with electro-magnet of kit, electro-magnet are located under the standing groove.

However, the whole blood nucleic acid extraction kit in this patent is a single tube holder, does not have a function of simultaneously detecting a plurality of tubes, and does not have a function of collecting and placing the whole blood nucleic acid extracted kit.

Therefore, a whole blood nucleic acid extraction kit based on nano magnetic beads is needed to meet the needs of people.

Disclosure of Invention

The utility model aims to provide a whole blood nucleic acid extraction kit based on nano magnetic beads, which solves the problems that the kit provided in the background art does not have the function of simultaneously detecting a plurality of test tubes and does not have the function of collecting and placing the kit after whole blood nucleic acid extraction.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a whole blood nucleic acid extraction kit based on nanometer magnetic bead, includes outer box, and open one side of outer box has the cavity, and the inside slidable mounting of cavity has the kit, and the opposite side of outer box is rotated and is installed the knob, and the one end fixed mounting of knob has the threaded rod, the below of kit is opened there is the screw hole with threaded rod assorted, and the threaded rod runs through outer box threaded connection at threaded hole.

The threaded rod fixedly installed at one end of the knob penetrates through the threaded hole formed in the lower portion of the kit and matched with the threaded hole, so that the kit can be pushed out of the kit, and the kit can be retracted into the kit after the sample is collected.

Preferably, the first spacing groove has all been seted up to the both sides of kit, and slidable mounting has first stopper in the first spacing groove, has arranged first spring in the first spacing groove, and the one end fixed mounting of first spring is on the inner wall in first spacing groove, and the other end fixed mounting of first spring is on first stopper.

Through the cooperation of first spacing groove, first stopper and first spring, have limit function when letting the kit be released from in the outer box, it is released to be restricted after reaching certain position to prevent that the kit from dropping in the outer box.

Preferably, the kit is internally and fixedly provided with a test tube table, the test tube table is provided with a plurality of test tube grooves, the inner wall of each test tube groove is provided with two mutually symmetrical second limit grooves, a second limit block is slidably arranged in each second limit groove, a second spring is arranged in each second limit groove, one end of each second spring is fixedly arranged in each second limit groove, and the other end of each second spring is fixedly arranged on each second limit block.

Through the cooperation of second spacing groove, second stopper and second spring to can let the test tube groove have the fixed function of centre gripping.

Preferably, a test tube is arranged in the test tube groove, the tube wall of the test tube is clamped by the second limiting block, the bottom of the test tube is contacted with the bottom of the test tube groove, and the top of the test tube is not higher than the top of the kit.

Through test tube second stopper centre gripping test tube wall to and the top of test tube is not higher than the kit top, thereby can be after gathering the sample with the kit withdraw in the outer box, and the test tube is difficult for being rocked thereby influences the testing result.

Preferably, a first supporting table is installed at one end of the bottom of the outer box, and a second supporting table corresponding to the first supporting table is installed at one side of the kit.

The first supporting table and the second supporting table can be used for stably placing the reagent box on the workbench after the reagent box is pushed out from the outer box.

Preferably, the other end of the bottom of the outer box is provided with a magnetic absorber, and the side surface of the outer box is provided with a magnetic absorption control button.

The magnetic force of the magnetic absorber is controlled by the magnetic control button.

Preferably, the thickness of the magnetic absorber is consistent with the space between the second supporting table and the kit, and the magnetic absorber is attached to the second supporting table after the kit is retracted into the outer box.

The magnetic absorber is attached to the second supporting table after the outer box is retracted, the thickness of the magnetic absorber is consistent with the space between the second supporting table and the kit, and the second supporting table and the outer box can be tightly closed through the magnetic absorber after the outer box is retracted, so that the effect of sealing the kit is achieved.

The beneficial effects of the utility model are as follows:

according to the utility model, one side of the outer box is provided with the cavity, the reagent box is slidably arranged in the cavity, the threaded rod penetrates through the outer box and is in threaded connection with the threaded hole arranged below the reagent box, one end of the threaded rod is fixedly arranged on the knob on the other side of the reagent box, and the threaded rod is driven to rotate through rotation of the knob, so that the reagent box can be pushed out for sample collection, and the reagent box can be retracted after sample collection is completed.

According to the utility model, the test tube table is fixedly arranged in the kit, the test tube table is provided with the plurality of test tube grooves, two second limit grooves which are symmetrical to each other are formed in the inner wall of the test tube groove, the second limit grooves are slidably provided with the second limit blocks, one ends of the second springs are fixedly arranged on the inner wall of the second limit grooves, and the other ends of the second springs are fixedly arranged on the second limit blocks, so that test tubes in the plurality of test tube grooves can be clamped and fixed to prevent the test tubes from shaking and falling out of the kit.

Drawings

FIG. 1 is a schematic diagram of a whole blood nucleic acid extraction kit based on nano magnetic beads;

FIG. 2 is a schematic diagram showing the cross-sectional structure of a whole blood nucleic acid extraction kit based on nano magnetic beads according to the present utility model;

FIG. 3 is a schematic diagram showing a side view and a cross section of a whole blood nucleic acid extraction kit based on magnetic nano-beads according to the present utility model;

fig. 4 is a schematic diagram of a rear view cross-section structure of a whole blood nucleic acid extraction kit based on nano magnetic beads according to the present utility model.

In the figure: 1. an outer case; 2. a cavity; 3. a kit; 4. a knob; 5. a threaded rod; 6. a threaded hole; 7. a first limit groove; 8. a first limiting block; 9. a first spring; 10. a test tube table; 11. a test tube groove; 12. the second limit groove; 13. a second limiting block; 14. a second spring; 15. a test tube; 16. a first support table; 17. a second support table; 18. a magnetic absorber; 19. a magnetic control button.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to fig. 1-4, a whole blood nucleic acid extraction kit based on nano magnetic beads comprises a kit 1, and is characterized in that: one side of the outer box 1 is provided with a cavity 2, a reagent box 3 is slidably arranged in the cavity 2, a knob 4 is rotatably arranged on the other side of the outer box 1, one end of the knob 4 is fixedly provided with a threaded rod 5, a threaded hole 6 matched with the threaded rod 5 is arranged below the reagent box 3, and the threaded rod 5 penetrates through the outer box 1 and is in threaded connection with the threaded hole 6.

One side through outer box 1 is opened there is cavity 2, and the inside slidable mounting of cavity 2 has kit 3, and threaded rod 5 runs through outer box 1 threaded connection in the screw hole 6 of seting up in the below of kit 3, and the one end fixed mounting of threaded rod 5 is on the knob 4 of kit 3 opposite side, rotates through knob 4 and drives threaded rod 5 and rotate to can release or withdraw kit 3 in outer box 1.

Referring to fig. 3, in the present utility model, the two sides of the kit 3 are provided with the first limiting groove 7, the first limiting groove 7 is slidably provided with the first limiting block 8, the first limiting groove 7 is internally provided with the first spring 9, one end of the first spring 9 is fixedly installed on the inner wall of the first limiting groove 7, and the other end of the first spring 9 is fixedly installed on the first limiting block 8.

First spacing groove 7 has been seted up through the both sides of kit 3, and sliding fit has first stopper 8 in first spacing groove 7, and the inner wall in first spacing groove 7 and first stopper 8 are connected to first spring 9 to let first stopper 8 have elastic expansion function, can carry out spacingly when pushing out outer box 1 to kit 3, prevent that kit 3 from falling out outer box 1.

Referring to fig. 4, in the utility model, a test tube table 10 is fixedly installed in a kit 3, a plurality of test tube grooves 11 are formed in the test tube table 10, two second limit grooves 12 which are symmetrical to each other are formed in the inner wall of the test tube groove 11, a second limit block 13 is slidably installed in the second limit groove 12, a second spring 14 is arranged in the second limit groove 12, one end of the second spring 14 is fixedly installed in the second limit groove 12, and the other end of the second spring 14 is fixedly installed on the second limit block 13.

Through fixed mounting in the kit 3 test tube platform 10, offer a plurality of test tube grooves 11 on the test tube platform 10, and through the inner wall in test tube groove 11 offered and see two second spacing grooves 12 of mutual symmetry, slidable mounting has second stopper 13 in the second spacing groove 12, the one end fixed mounting of second spring 14 is at the inner wall in second spacing groove 12, the other end fixed mounting of second spring 14 is on second stopper 13 to can let a plurality of test tube grooves 11 have the centre gripping fixed function.

Referring to fig. 4, in the utility model, a test tube 15 is installed in a test tube groove 11, the tube wall of the test tube 15 is clamped by a second limiting block 13, the bottom of the test tube 15 is contacted with the bottom of the test tube groove 11, and the top of the test tube 15 is not higher than the top of a kit 3.

Through test tube groove 11 has the centre gripping fixed function to let test tube 15 in the test tube groove 11 can be placed in test tube groove 11 steadily, be difficult for rocking and fall out test tube groove 11, the bottom of test tube 15 contacts with test tube groove 11 bottom and the top of test tube 15 is not higher than the top of kit 3, thereby can be after collecting the sample can retrieve kit 3 in outer box 1.

Referring to fig. 1 to 4, in the present utility model, a first support table 16 is installed at one end of the bottom of the outer box 1, and a second support table 17 corresponding to the first support table 16 is installed at one side of the kit 3.

By means of the first support table 16 and the second support table 17, the reagent kit 3 and the outer box 1 can be kept horizontally and stably placed on the workbench when the reagent kit 3 is used.

Referring to fig. 1 and 3, in the present utility model, a magnetic absorber 18 is installed at the other end of the bottom of the outer case 1, and a magnetic control button 19 is installed at the side of the outer case 1.

The magnetic force of the magnetic attractor 18 can be controlled by a magnetic attraction control button 19 mounted on the side surface of the outer case 1.

Referring to fig. 1 to 4, in the present utility model, the thickness of the magnetic absorber 18 corresponds to the space between the second support table 17 and the reagent vessel 3, and the magnetic absorber 18 is attached to the second support table 17 after the reagent vessel 3 is retracted into the outer vessel 1.

The thickness of the magnetic absorber 18 is consistent with the space between the second supporting table 17 and the reagent kit 3, and the magnetic absorber 18 is attached to the second supporting table 17 after the reagent kit 3 is retracted into the outer kit 1, so that the reagent kit 3 can be retracted into the outer kit 1 after the sample collection work is completed, the magnetic force of the magnetic absorber 18 is used for absorbing the second supporting table 17 to seal the reagent kit 3, and the reagent kit 3 is prevented from being separated from the outer kit 1 due to shaking.

The present utility model is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present utility model and the inventive concept thereof, can be replaced or changed within the scope of the present utility model.

Claims

1. Whole blood nucleic acid extraction kit based on nanometer magnetic bead, including outer box (1), its characterized in that: one side of outer box (1) is opened there is cavity (2), and the inside slidable mounting of cavity (2) has kit (3), and knob (4) are installed in the opposite side rotation of outer box (1), and one end fixed mounting of knob (4) has threaded rod (5), the below of kit (3) is opened there is threaded hole (6) with threaded rod (5) assorted, and threaded rod (5) run through outer box (1) threaded connection in threaded hole (6).

2. The whole blood nucleic acid extraction kit based on the nano magnetic beads according to claim 1, wherein: the kit comprises a kit body, wherein a first limiting groove (7) is formed in two sides of the kit body (3), a first limiting block (8) is arranged in the first limiting groove (7) in a sliding mode, a first spring (9) is arranged in the first limiting groove (7), one end of the first spring (9) is fixedly arranged on the inner wall of the first limiting groove (7), and the other end of the first spring (9) is fixedly arranged on the first limiting block (8).

3. The whole blood nucleic acid extraction kit based on the nano magnetic beads according to claim 1, wherein: the kit comprises a kit body, a reagent box (3), a test tube table (10) fixedly installed in the kit body, a plurality of test tube grooves (11) are formed in the test tube table (10), two second limit grooves (12) which are symmetrical to each other are formed in the inner wall of the test tube groove (11), a second limit block (13) is arranged in the second limit groove (12) in a sliding mode, a second spring (14) is arranged in the second limit groove (12), one end of the second spring (14) is fixedly installed in the second limit groove (12), and the other end of the second spring (14) is fixedly installed on the second limit block (13).

4. A whole blood nucleic acid extraction kit based on nano magnetic beads according to claim 3, wherein: install test tube (15) in test tube groove (11), the pipe wall of test tube (15) is by second stopper (13) centre gripping, and the bottom of test tube (15) contacts with test tube groove (11) bottom, and the top of test tube (15) is not higher than the top of kit (3).

5. The whole blood nucleic acid extraction kit based on the nano magnetic beads according to claim 1, wherein: a first supporting table (16) is arranged at one end of the bottom of the outer box (1), and a second supporting table (17) corresponding to the first supporting table (16) is arranged on one side of the kit (3).

6. The whole blood nucleic acid extraction kit based on the nano magnetic beads according to claim 1, wherein: the other end of the bottom of the outer box (1) is provided with a magnetic absorber (18), and the side surface of the outer box (1) is provided with a magnetic absorption control button (19).

7. The whole blood nucleic acid extraction kit based on the nano magnetic beads according to claim 6, wherein: the thickness of the magnetic absorber (18) is consistent with the space between the second supporting table (17) and the kit (3), and the magnetic absorber (18) is attached to the second supporting table (17) after the kit (3) is retracted into the outer box (1).