CN219224318U - Malaria blood smear automatic dyeing device - Google Patents

Abstract

The utility model provides an automatic malaria blood smear dyeing device, wherein a dyeing groove is formed in a blood smear dyeing instrument, a reagent placing table is arranged on one side of the blood smear dyeing instrument, a methanol storage bottle, a dye liquor mother liquor bottle and a diluent bottle are arranged on the reagent placing table, the dye liquor mother liquor bottle and the diluent bottle of the methanol storage bottle are communicated into the dyeing groove, a blood smear placing component is placed in the dyeing groove, multiple groups of blood smears can be placed in the blood smear placing component at the same time, and multiple groups of blood smears are placed in the blood smear placing component in a way that thick blood films on the blood smear placing component are high and thin blood films are low. The utility model can realize the simultaneous dyeing operation of a plurality of malaria blood smears, effectively improve the dyeing efficiency of the malaria blood smears and further ensure the dyeing quality.

Description

Malaria blood smear automatic dyeing device

Technical Field

The utility model mainly relates to the technical field of experimental equipment, in particular to the technical field of automatic blood smear dyeing devices, and specifically relates to an automatic malaria blood smear dyeing device.

Background

The automatic blood smear dyeing device has the advantages that the operation of artificial blood smear dyeing is replaced by a machine through a mechanical structure, so that the influence of human factors on the blood smear dyeing process can be reduced, various test solutions can be accurately titrated, the self safety of operators can be ensured, and the automatic blood smear dyeing device has a great effect on the research of the biomedical field.

The utility model patent with the bulletin number of CN209802798U discloses a novel automatic blood smear dyeing device, which comprises a blood smear dyeing instrument, a second spring and a blood smear, wherein a working bin is fixedly arranged on the upper side of the blood smear dyeing instrument, an upper fixing plate is arranged in the working bin, a first spring is embedded on the upper fixing plate, a first connecting rod is fixedly connected to the first spring, the second spring is embedded on the upper fixing plate, a second clamping plate is connected to the second spring through a second connecting rod, the blood smear is clamped between the first clamping plate and the second clamping plate, a lower fixing plate is fixedly connected to the middle part of the lower side of the upper fixing plate through a connecting column, and a sponge block is embedded on the lower fixing plate. The clamping device can clamp and fix blood smears with different thicknesses, can effectively reduce the contact of blood smear reagents and the clamping plates, and can avoid the situation of damage of the blood smears due to overlarge clamping force due to a soft rubber pad, thereby greatly improving the practicability of the device.

Above-mentioned patent is fixed through setting up two sets of splint that have telescopic holding structure to blood smear, utilizes the flexible characteristic of spring self, cooperation articulated connecting rod, carries out the slant installation with two sets of splint, and the upside interval of two sets of splint is greater than the downside for blood smear of different thickness is more easily inserted and is carried out the centre gripping between two sets of splint and fix, but above-mentioned patent can only carry out the dyeing work to two blood smears simultaneously, when the blood smear that needs to dye is more, above-mentioned patent obviously is insufficient to satisfy the user demand, causes malaria blood smear dyeing efficiency to descend, brings inconvenience for the user.

Disclosure of Invention

The utility model mainly provides an automatic malaria blood smear staining device which is used for solving the technical problems in the background technology.

The technical scheme adopted for solving the technical problems is as follows:

the utility model provides an automatic dyeing device of malaria blood smear, includes blood smear dyeing appearance, the inside dye vat that has of blood smear dyeing appearance, blood smear dyeing appearance one side is equipped with the reagent and places the platform, the reagent is placed bench and is provided with methyl alcohol storage bottle, dye liquor mother liquor bottle and diluent bottle, methyl alcohol storage bottle, dye liquor mother liquor bottle and diluent bottle all communicate extremely in the dye vat, blood smear placement module has been placed in the dye vat, blood smear placement module can place multiunit blood smear simultaneously, and the multiunit blood smear all is in with thick blood membrane high position, thin blood membrane low place on it place in the blood smear placement module.

In some embodiments, the blood smear placing component comprises a film loading box placed in the staining groove, a plurality of placing grooves are uniformly distributed on the film loading box, the placing grooves are inclined grooves with one high end and one low end, through holes communicated with the bottom of the film loading box are formed in the low position, and the bottom of the film loading box is communicated with the methanol storage bottle.

In some embodiments, the chip box comprises an upper frame and a lower frame, the upper frame is embedded in the lower frame, the surface of the upper frame is uniformly distributed with a plurality of placing grooves, one corner of the lower frame is provided with a formaldehyde liquid inlet, and the other opposite corner is provided with a liquid outlet.

In some embodiments, the top of the blood smear staining instrument is provided with a cover plate, the upper surface of the cover plate is provided with a dye liquor inlet, and the dye liquor inlet is connected with the dye liquor mother liquor bottle and the diluent bottle and is communicated to an internal staining tank.

In some embodiments, the cover plate has a plurality of spray holes formed in a lower surface thereof, each spray hole corresponding to a set of the blood smears.

In some embodiments, the cover plate is hingedly mounted to the blood smear staining apparatus and a hand-held groove is reserved at the top.

In some embodiments, the blood smear is coated with the thick blood film and the thin blood film, and a marking area is reserved on one side of the thick blood film.

In some embodiments, micropumps are provided on top of the methanol storage bottle, dye liquor mother liquor bottle and diluent bottle.

In some embodiments, the reagent placement table is further provided with a waste bottle, and a micropump is provided on top of the waste bottle.

In some embodiments, the placement slots are in a 4 x 4 arrangement on the card case.

Compared with the prior art, the utility model has the beneficial effects that at least:

1. according to the blood smear placing assembly, a plurality of blood smear placing grooves are formed, so that a plurality of malaria blood smears can be simultaneously dyed, and the dyeing efficiency of the malaria blood smears is improved.

2. Because the thin blood film is fixed by methanol before dyeing, the blood smear is obliquely placed, the position of the thin blood film is lower, the thick blood film is higher, formaldehyde is conveniently soaked in the thin blood film when formaldehyde solution is introduced from the lower part, the thick blood film is not influenced, and the dyeing quality is ensured.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those of ordinary skill in the art that the drawings in the following description are exemplary only and that other implementations can be obtained from the extensions of the drawings provided without inventive effort.

The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the utility model, which is defined by the claims, but rather by the claims.

FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present utility model;

FIG. 2 is a schematic view of a cover plate according to an embodiment of the utility model;

FIG. 3 is a schematic view showing the positional relationship between a blood smear placement module and a cover plate according to an embodiment of the present utility model;

FIG. 4 is a schematic view of a structure of a card case according to an embodiment of the present utility model;

FIG. 5 is an exploded view of a cartridge according to an embodiment of the present utility model;

FIG. 6 is a schematic view of the bottom structure of a card case according to an embodiment of the present utility model;

FIG. 7 is a schematic view of a cover plate unfolding structure (for placing a chip case) according to an embodiment of the utility model;

fig. 8 is a schematic view of a blood smear according to an embodiment of the present utility model.

In the figure: 1. a blood smear staining instrument; 101. a support base; 102. a dyeing tank; 103. a slot; 2. a reagent placement table; 201. a micropump; 202. methanol storage bottle; 203. dye liquor mother liquor bottle; 204. a diluent bottle; 205. a waste liquid bottle; 3. a blood smear placement assembly; 301. a loading box; 302. a placement groove; 303. a through hole; 304. a lower frame; 305. a clamping piece; 306. a formaldehyde liquid inlet; 307. a liquid outlet; 308. an upper frame; 4. blood smear; 401. thick blood film; 402. a thin blood film; 403. a marking area; 5. a cover plate; 501. spraying holes; 502. a hand buckling groove; 503. dye liquor inlet.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the embodiments of the present utility model will be described in further detail with reference to the embodiments and the accompanying drawings. The exemplary embodiments of the present utility model and their descriptions herein are for the purpose of explaining the present utility model, but are not to be construed as limiting the utility model.

In the present utility model, unless explicitly specified and limited otherwise, the terms "disposed," "connected," "communicating," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

It should be understood that the terms "comprises/comprising," "consists of … …," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a product, apparatus, process, or method that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such product, apparatus, process, or method as desired. Without further limitation, an element defined by the phrases "comprising/including … …," "consisting of … …," and the like, does not exclude the presence of other like elements in a product, apparatus, process, or method that includes the element.

It is further understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship based on that shown in the drawings, merely to facilitate describing the present utility model and to simplify the description, and do not indicate or imply that the devices, components, or structures referred to must have a particular orientation, be configured or operated in a particular orientation, and are not to be construed as limiting the present utility model. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

The implementation of the present utility model will be described in detail with reference to the preferred embodiments and specific drawings.

Referring generally to fig. 1 to 8, an automatic malaria blood smear staining apparatus provided in an embodiment of the present utility model mainly includes a blood smear staining apparatus 1, which is easy to understand, and is used for staining malaria blood smears, and the stained malaria blood smears can be used for microscopic examination after being placed in a loading box and dried.

In the utility model, one side of the blood smear staining apparatus 1 is provided with the reagent placing table 2, and the reagent placing table 2 is preferably arranged at the left side or the right side of the blood smear staining apparatus 1, and can be a separate component or an integrated structure with the blood smear staining apparatus 1, and the specific structure and the shape are not strictly limited. The reagent placement table 2 is provided with a methanol storage bottle 202, a dye liquor mother liquor bottle 203 and a diluent bottle 204, for example, arranged in a row, and sequentially arranged from left to right, and the reagent placement table 2 may be formed with a placement groove in which each solution bottle is placed to prevent the solution bottle from being poured.

In the utility model, the tops of a methanol storage bottle 202, a dye liquor mother liquor bottle 203 and a diluent bottle 204 are respectively provided with a micro pump 201, and are communicated with the interior of a blood smear staining instrument 1, and corresponding liquid is conveyed into the blood smear staining instrument 1 by the micro pumps 201. The micropump 201 may be of a conventional construction. Jib's dye liquor is preferably used in the dye liquor mother liquor bottle 203.

In the utility model, the blood smear dyeing instrument 1 is provided with the dyeing groove 102, the dyeing groove 102 is arranged in the middle area inside the blood smear dyeing instrument 1, and can be specifically a hollow cavity arranged on the blood smear dyeing instrument 1, and the blood smear dyeing instrument 1 can be easily understood to be provided with a control and display panel, a power interface and the like on one side, and the blood smear dyeing instrument 1 is provided with the base 101 at the bottom, and the four corners of the bottom of the base 101 can be provided with supporting legs or movable wheels. The blood smear placing component 3 is placed in the dyeing groove 102, and the blood smear placing component 3 can simultaneously place a plurality of groups of blood smears 4 so as to simultaneously perform a plurality of groups of blood smear examinations at one time, improve the test efficiency and meet the test requirement.

In the present utility model, a plurality of sets of blood smears 4 are placed in the blood smear placing component 3 with the thick blood film 401 thereon in a high position and the thin blood film 402 thereon in a low position. So set up, when fixed thin blood film through methyl alcohol before dyeing, because thick blood film is in the high position, be convenient for carry out formaldehyde to the thin blood film when letting in formaldehyde solution from the below and soak, can not influence thick blood film simultaneously, ensure dyeing quality.

Specifically, in some embodiments, referring to fig. 3 and 4, the blood smear placement assembly 3 includes a wafer cassette 301, which in use, is placed within the staining trough 102 for placement of the blood smear 4.

In some embodiments, as shown in fig. 4, a plurality of placement grooves 302 are uniformly distributed on the wafer box 301, the placement grooves 302 are inclined grooves with one high end and one low end (the right high end and the left low end in the figure), and through holes 303 communicated with the bottom of the wafer box 301 are formed at the low end, and the bottom of the wafer box 301 is communicated with the methanol storage bottle 202. Through the through hole 303 communicated with the bottom of the chip box 301 is formed at the lower position, formaldehyde solution at the lower part is allowed to enter the placing groove 302 through the through hole 303, so that the thin blood film 402 of the blood smear 4 is soaked, and meanwhile, the thick blood film 401 is not soaked.

In some embodiments, as shown in fig. 5 and 6, the packaging box 301 includes an upper frame 308 and a lower frame 304, the upper frame 308 is embedded in the lower frame 304, a plurality of placing grooves 302 are uniformly distributed on the surface of the upper frame 308, and a formaldehyde liquid inlet 306 and a liquid outlet 307 are disposed on the lower frame 304. By designing the wafer cassette 301 in the form of an upper frame 308 and a lower frame 304, and designing the formaldehyde liquid inlet 306 and the liquid outlet 307 on the lower frame 304, formaldehyde is injected from the bottom of the wafer cassette, so that formaldehyde solution in the methanol storage bottle 202 is introduced into the placing groove 302 from the lower part, the formaldehyde solution wets the thin film of the blood smear 4 from the through hole 303 of the placing groove 302 from the lower part, covers the thin film, and formaldehyde enters the waste liquid bottle from the bottom Kong Fangdiao of the wafer cassette.

Preferably, the formaldehyde inlet 306 is disposed at one corner of the lower frame 304, and the liquid outlet 307 is disposed at the other opposite corner of the lower frame 304, i.e. diagonally opposite corner, so as to ensure that formaldehyde can cover the placement groove 302 in all areas from the bottom.

As shown in fig. 8, the blood smear 4 includes a thick blood film 401 and a thin blood film 402, wherein the thick blood film 401 needs to be dyed by using a dye solution prepared by using a jetty dye solution mother solution and a diluent, the thin blood film 402 only needs to be soaked and wetted by formaldehyde, the thick blood film 401 is placed corresponding to a high-level region of the placing groove 302, the thin blood film 402 is placed corresponding to a low-level region of the placing groove 302, specifically, corresponding to the through hole 303, and a marking region 403 is reserved on one side of the thick blood film 401 of the blood smear 4 for marking relevant information in a test.

In some embodiments, with continued reference to fig. 2 and 3, the top of the blood smear staining apparatus 1 is further provided with a cover plate 5, and a dye liquor inlet 503 is provided on the cover plate 5, and the dye liquor inlet 503 connects the dye liquor mother liquor bottle 203 and the dilution liquor bottle 204 and communicates with the internal placing groove 302. The Gibbs' stain and diluent are fed into the wafer cassette from above, immersed in the thick blood film 401, and kept clear of the marking zone 403.

In some embodiments, with continued reference to fig. 7, the lower surface of the cover plate 5 further includes a plurality of spray holes 501, each spray hole 501 being positioned to correspond to a set of blood smears 4. Dye liquor enters from a dye liquor inlet 503 and then enters the placing groove 302 through a spray hole 501 so as to realize accurate, simultaneous and uniform dyeing of each blood smear 4.

Preferably, the cover plate 5 is hinged to the blood smear staining apparatus 1, and a hand-fastening groove 502 is reserved on one side of the top to facilitate the operator to open.

As further shown in fig. 2, a waste liquid bottle 205 is further provided on the reagent holding table 2, and a micropump 201 is provided on the top thereof so that waste liquid generated during and after the completion of the test is discharged and recovered through a liquid discharge port 307.

As shown in fig. 6, a clamping piece 305 is arranged at the bottom of the lower frame 304, slots 103 are formed at four corners corresponding to the inner edge of the staining groove 102, and the clamping piece 305 is clamped and fixed with the slots 103, so as to position and fix the blood smear placing component 3 in the staining groove 102. The clamping members 305 may be clamping bars, clamping blocks, etc. disposed at four corners of the bottom of the lower frame 304.

In the present utility model, 16 placement slots 302 capable of placing blood smears are arranged on the surface of the wafer box 301 in a 4×4 array arrangement, namely, 4 rows×4 columns. The staff can once only place 16 blood smears 4 in corresponding standing groove 302 one by one, can realize simultaneously that a plurality of malaria blood smears carry out the simultaneous dyeing operation, improves the dyeing efficiency of malaria blood smears.

The specific operation of the utility model comprises links of loading, soaking, dyeing, cleaning, drying and the like, and the specific steps are as follows:

and (3) loading: the staff will correspond various solution reagent bottles that need to use and place on reagent placement table 2 in proper order to communicate each reagent bottle through coupling hose, then the staff opens apron 5 through hand catching groove 502, take out loading box 301, the staff can once only place a plurality of blood smears 4 one by one in corresponding standing groove 302, can realize simultaneously that a plurality of malaria blood smears carry out the dyeing operation simultaneously, improve malaria blood smear's dyeing efficiency, after blood smear is placed, the staff is in loading into dyeing groove 102 loading into loading box 301 through joint spare 305, and close apron 5, when apron 5 is closed, spraying hole 501 on it corresponds a set of standing groove 302 just, namely corresponds a blood smear 4.

And (3) formaldehyde infiltration: formaldehyde in the methanol storage bottle 202 is sucked by the micropump 201, so that the formaldehyde enters the lower frame 304 through the formaldehyde liquid inlet 306, and the formaldehyde solution gradually submerges the thin blood film 402 in the lower frame 304 from bottom to top to moisten the thin blood film 402. After the thin film 402 is wetted, the formaldehyde waste liquid is discharged into the waste liquid bottle 205 through the liquid outlet 307 by the micropump 201 and collected.

Dyeing: after the thin blood film 402 is dried, the solution in the dye liquor mother liquor bottle 203 and the solution in the dilution liquor bottle 204 are sucked through the micro pump 201, the dye liquor is formed after mixing and is dripped into the thick blood film 401 area through the dye liquor inlet 503 via the spray holes 501, after dyeing for 10-30 minutes according to different configuration concentrations, the dye liquor is discharged into the waste liquor bottle 205 through the liquid outlet 307 through the micro pump 201 for collection.

Cleaning and drying: finally, the cover plate 5 is opened by the hand-buckling groove 502, clean water is injected into the wafer box 301, the wafer box is placed for 10 seconds, the rinsed water is discharged into the waste liquid bottle 205, the operation is repeated, and the dyed blood smear is placed in the wafer box for drying, so that the blood smear can be used for microscopic examination.

It is easy to understand by those skilled in the art that the above preferred embodiments can be freely combined and overlapped without conflict.

The foregoing description of the preferred embodiments of the utility model is not intended to limit the utility model to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the utility model.

Claims (10)

1. An automatic malaria blood smear staining device comprises a blood smear staining instrument (1), and is characterized in that: blood smear dyeing appearance (1) inside has dyeing tank (102), blood smear dyeing appearance (1) one side is equipped with reagent and places platform (2), be provided with methyl alcohol storage bottle (202), dye liquor mother liquor bottle (203) and diluent bottle (204) on reagent and place platform (2), methyl alcohol storage bottle (202), dye liquor mother liquor bottle (203) and diluent bottle (204) all communicate to in dyeing tank (102), placed blood smear in dyeing tank (102) and placed subassembly (3), blood smear is placed subassembly (3) and can be placed multiunit blood smear (4) simultaneously, and multiunit blood smear (4) all are in with thick blood membrane (401) high order, thin blood membrane (402) low place on it place in blood smear place subassembly (3).

2. An automatic staining apparatus for malaria blood smears according to claim 1, wherein: blood smear places subassembly (3) including place in dress box (301) in dyeing groove (102), evenly laid a plurality of standing grooves (302) on dress box (301), standing groove (302) are chute of one end high position, one end low position to through-hole (303) of intercommunication dress box (301) bottom are seted up to low position department, dress box (301) bottom intercommunication methyl alcohol storage bottle (202).

3. An automatic staining apparatus for malaria blood smears according to claim 2, wherein: the chip box (301) comprises an upper frame (308) and a lower frame (304), wherein the upper frame (308) is embedded in the lower frame (304), a plurality of placing grooves (302) are uniformly distributed on the surface of the upper frame (308), one corner of the lower frame (304) is provided with a formaldehyde liquid inlet (306), and the other opposite corner is provided with a liquid outlet (307).

4. An automatic staining apparatus for malaria blood smears according to claim 1, wherein: the blood smear dyeing instrument (1) top is provided with apron (5), apron (5) upper surface is equipped with dye liquor inlet (503), dye liquor inlet (503) connect dye liquor mother liquor bottle (203) and diluent bottle (204) to communicate to inside dyeing groove (102).

5. An automatic staining apparatus for malaria blood smears according to claim 4, wherein: a plurality of spraying holes (501) are formed in the lower surface of the cover plate (5), and each spraying hole (501) corresponds to one group of blood smears (4).

6. An automatic staining apparatus for malaria blood smears according to claim 4, wherein: the cover plate (5) is hinged on the blood smear staining instrument (1), and a hand buckling groove (502) is reserved at the top.

7. An automatic staining apparatus for malaria blood smears according to claim 1, wherein: the blood smear (4) is coated with the thick blood film (401) and the thin blood film (402), and a marker region (403) is reserved on one side of the thick blood film (401).

8. An automatic staining apparatus for malaria blood smears according to claim 1, wherein: the tops of the methanol storage bottle (202), the dye liquor mother liquor bottle (203) and the diluent bottle (204) are respectively provided with a micropump (201).

9. An automatic staining apparatus for malaria blood smears according to claim 1, wherein: the reagent placing table (2) is also provided with a waste liquid bottle (205), and the top of the waste liquid bottle (205) is provided with a micropump (201).

10. An automatic staining apparatus for malaria blood smears according to claim 2, wherein: the placement grooves (302) are arranged in a 4 x 4 arrangement on the wafer cassette (301).

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