CN219194989U - Cell culture apparatus - Google Patents
Cell culture apparatus Download PDFInfo
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- CN219194989U CN219194989U CN202223012600.9U CN202223012600U CN219194989U CN 219194989 U CN219194989 U CN 219194989U CN 202223012600 U CN202223012600 U CN 202223012600U CN 219194989 U CN219194989 U CN 219194989U
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Abstract
The utility model relates to the technical field of cell culture, in particular to cell culture equipment, and aims to solve the problem of single function of the existing cell culture equipment. To this end, the cell culture apparatus of the present utility model comprises a cell culture section for performing a cell culture work, a cell partition section, a sample buffer section, a first transport means, and a second transport means; the cell liquid division area is used for carrying out liquid division treatment on the cell stock solution, and is communicated with the cell culture area; the first conveying device is used for conveying the cell sap subjected to the liquid separation from the cell sap separation region to the cell culture region; the sample buffer area is used for storing the cultured cell sample and is communicated with the cell culture area; the second conveying device is used for conveying the cell sample from the cell culture area to the sample buffer area. Through such setting for cell culture equipment collects buffering, divides liquid, cultivates in an organic wholely, has greatly enriched cell culture equipment's function, and user's use experience is better.
Description
Technical Field
The utility model relates to the technical field of cell culture, and particularly provides cell culture equipment.
Background
With the rapid development of medical and medical treatment, life and health industries and the like, cell medical treatment plays an increasingly important role, and the requirements of people on cells with high quality, uniformity and stability sources are higher and higher.
In the prior art, cell culture work is mainly completed by cell culture equipment, however, the existing cell culture equipment can only perform cell culture work generally, and has single function and poor user experience.
Accordingly, there is a need in the art for a new solution to the above-mentioned problems.
Disclosure of Invention
The utility model aims to solve the technical problem that the existing cell culture equipment has single function.
In a first aspect, the present utility model provides a cell culture apparatus comprising:
a cell culture section for performing a cell culture work;
the cell liquid separation area is used for carrying out liquid separation treatment on the cell stock solution and is communicated with the cell culture area;
a first conveying device for conveying the cell sap separated from the cell sap separation region to the cell culture region;
a sample buffer zone for storing a cultured cell sample, the sample buffer zone being in communication with the cell culture zone; and
and the second conveying device is used for conveying the cultured cell sample from the cell culture area to the sample buffer area.
In a preferred embodiment of the above cell culture apparatus, the cell division area is located above the cell culture area, and a bottom of the cell division area is communicated with a top of the cell culture area.
In a preferred embodiment of the above cell culture apparatus, the first conveying device is a vertical mechanical arm.
In a preferred embodiment of the above cell culture apparatus, the sample buffer is located above the cell culture region, and the bottom of the sample buffer is communicated with the top of the cell culture region.
In a preferred embodiment of the above cell culture apparatus, the second conveying device is a six-axis robot.
In a preferred embodiment of the above cell culture apparatus, the six-axis robot is installed in the sample buffer.
In a preferred embodiment of the above cell culture apparatus, the temperature of the environment in the sample buffer is 2 to 6 degrees.
In a preferred embodiment of the above cell culture apparatus, the ambient temperature in the sample buffer is 4 degrees.
In the preferred technical scheme of the cell culture apparatus, the cell division liquid region and the sample buffer region are respectively located at the left side and the right side of the cell culture region, and the left side and the right side of the cell culture region are respectively communicated with the cell division liquid region and the sample buffer region.
In the preferred technical scheme of the cell culture apparatus, the cell division liquid region and the sample buffer region are respectively located at front and rear sides of the cell culture region, and the front and rear sides of the cell culture region are respectively communicated with the cell division liquid region and the sample buffer region.
Under the condition of adopting the technical scheme, the cell culture equipment comprises a cell culture area, a cell liquid division area, a sample buffer area, a first conveying device and a second conveying device, wherein the cell culture area is used for carrying out cell culture work; the cell liquid separation area is used for carrying out liquid separation treatment on the cell stock solution, and is communicated with the cell culture area; the first conveying device is used for conveying the cell sap subjected to the liquid separation from the cell sap separation region to the cell culture region; the sample buffer area is used for storing the cultured cell sample, and is communicated with the cell culture area; the second conveying device is used for conveying the cultured cell sample from the cell culture area to the sample buffer area. Through the arrangement, the cell culture equipment integrates the functions of buffering, liquid separation and culture, greatly enriches the functions of the cell culture equipment, can reduce the operation of personnel, avoids the pollution problem caused by repeated transfer, and has high efficiency and better use experience of users.
Drawings
Preferred embodiments of the present utility model are described below with reference to the accompanying drawings, in which:
FIG. 1 is a schematic diagram of the structure of a cell culture apparatus of the present utility model;
FIG. 2 is a schematic diagram showing the structure of a liquid separation device of the cell culture apparatus of the present utility model.
List of reference numerals:
1. a cell culture zone; 2. a cell division area; 3. a sample buffer; 4. a first conveying device; 5. a second conveying device; 6. a cell liquid separation device; 61. a tube moving and cover opening mechanism; 62. a liquid separating mechanism; 63. a fixing seat; 631. a fixing hole; 7. a case; 8. a horizontal partition; 81. an opening; 9. a vertical partition.
Detailed Description
Preferred embodiments of the present utility model are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present utility model, and are not intended to limit the scope of the present utility model.
It should be noted that, in the description of the present utility model, terms such as "inner", "outer", "upper", "lower", "top", "bottom", and the like, which indicate a direction or a positional relationship, are based on the direction or the positional relationship shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the apparatus or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present utility model, unless explicitly stated and limited otherwise, the terms "disposed," "connected," and "mounted" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected. The specific meaning of the above terms in the present utility model can be understood by those skilled in the art according to the specific circumstances.
The prior cell culture equipment has the problem of single function based on the background technology. The utility model provides cell culture equipment, which aims to add a cell liquid separation area for performing cell liquid separation work and a sample buffer area for storing cell samples to the cell culture equipment so as to increase the functions of the cell culture equipment and improve the use experience of a user.
Specifically, the cell culture apparatus of the present utility model comprises a cell culture section, a cell division section, a sample buffer section, a first conveying device and a second conveying device.
Wherein, the cell culture area is used for carrying out cell culture work; the cell liquid separation area is used for carrying out liquid separation treatment on the cell stock solution, and is communicated with the cell culture area; the first conveying device is used for conveying the cell sap subjected to the liquid separation from the cell sap separation region to the cell culture region; the sample buffer area is used for storing the cultured cell sample, and is communicated with the cell culture area; the second conveying device is used for conveying the cultured cell sample from the cell culture area to the sample buffer area.
The staff puts into the cell division liquid district with the sample pipe that holds cell stoste in, is provided with cell division liquid device in cell division liquid district, and cell division liquid device can distribute the cell stoste in the sample pipe in a plurality of cultivate the pipe to place a plurality of cultivate the pipe in the sample box, after the branch liquid is accomplished, carry the sample box to cell culture district by first conveyor and cultivate.
The cell culture device is arranged in the cell culture area, the culture tube is placed in the cell culture device for culture, and after the cell culture is completed, the cultured cell sample is conveyed into the sample buffer area for storage by the second conveying device. The user may periodically withdraw the cell sample stored in the sample buffer.
By additionally arranging the cell liquid dividing region and the sample buffer region for the cell culture equipment, the cell culture equipment integrates buffer storage, liquid dividing and culture, greatly enriches the functions of the cell culture equipment, can reduce the operation of personnel, avoid the pollution problem existing in repeated transfer, has high efficiency, and has better use experience for users.
It should be noted that, in practical applications, those skilled in the art may make the cell division area and the sample buffer area located on the same side of the cell culture area, or may make the cell culture area located between the cell division area and the sample buffer area, etc., and such adjustment and change of the positional relationship of the cell culture area, the cell division area and the sample buffer area do not deviate from the principle and scope of the present utility model, and should be limited in the scope of protection of the present utility model.
In a first preferred case, the cell division area and the sample buffer area are both located at the left and right sides of the cell culture area, and the left and right sides of the cell culture area are respectively communicated with the cell division area and the sample buffer area.
In a second preferred case, the cell division area and the sample buffer area are respectively located at the front and rear sides of the cell culture area, and the front and rear sides of the cell culture area are respectively communicated with the cell division area and the sample buffer area.
In a third preferred case, both the cell-separating zone and the sample-buffering zone are located above the cell-culturing zone.
In a fourth preferred case, only the cell division is located above the cell culture zone.
In a fifth preferred case, only the sample buffer is located above the cell culture zone.
The technical scheme of the present utility model will be further described below with reference to fig. 1 and 2 by taking an example that the cell division area and the sample buffer area are both located above the cell culture area.
As shown in fig. 1, the cell culture apparatus of the present utility model comprises a cell culture zone 1, a cell division zone 2, a sample buffer zone 3, a first conveying device 4 and a second conveying device 5, wherein the cell division zone 2 is located above the cell culture zone 1, the bottom of the cell division zone 2 is communicated with the top of the cell culture zone 1, the sample buffer zone 3 is also located above the cell culture zone 1, and the bottom of the sample buffer zone 3 is communicated with the top of the cell culture zone 1.
Wherein the cell culture area 1 is used for performing cell culture work; the cell liquid separation area 2 is used for carrying out liquid separation treatment on the cell stock solution; the first conveying device 4 is used for conveying the cell liquid with separated liquid from the cell liquid separating area 2 to the cell culture area 1; the sample buffer area 3 is used for storing the cell samples after culture; the second transporting device 5 is used for transporting the cultured cell sample from the cell culture zone 1 to the sample buffer zone 3.
The staff puts into cell division liquid district 2 with the sample pipe that holds cell stoste, is provided with cell division liquid device 6 in cell division liquid district 2, and cell division liquid device 6 can distribute the cell stoste in the sample pipe in a plurality of cultivate the pipe to place a plurality of cultivate the pipe in the sample box, after the branch liquid is accomplished, carry the sample box to cell culture district 1 by first conveyor 4 and cultivate.
A cell culture device (not shown) is arranged in the cell culture area 1, a culture tube is placed in the cell culture device for culture, and after the cell culture is completed, a cultured cell sample is conveyed into the sample buffer area 3 for storage by the second conveying device 5. The user can take out the cell samples stored in the sample buffer 3 at regular intervals.
Illustratively, the cell culture section 1, the cell division section 2 and the sample buffer section 3 are disposed in the same casing 7, the internal space of the casing 7 is divided into three independent working sections (cell culture section 1, cell division section 2 and sample buffer section 3) by a horizontal partition 8 and a vertical partition 9, the cell culture section 1 is located below the horizontal partition 8, the cell division section 2 and the sample buffer section 3 are both located above the horizontal partition 8, the cell division section 2 is located on the right side of the vertical partition 9, and the sample buffer section 3 is located on the left side of the vertical partition 9. The horizontal partition plate 8 is provided with two openings 81, the cell culture area 1 is communicated with the cell liquid division area 2 through one opening 81, the cell culture area 1 is communicated with the sample buffer area 3 through the other opening 81, and an electric door (not shown in the figure) is arranged at each opening 81, so that the opening 81 can be closed through the electric door.
The temperature of the environment in the cell culture zone 1 is typically set to 37 degrees, the temperature of the environment in the cell division zone 2 is typically set to normal temperature, 22 to 25 degrees, and the temperature of the environment in the sample buffer zone 3 is typically set to 4 degrees.
It should be noted that, the ambient temperature in the cell culture section 1 is not limited to 37 degrees as described above, and those skilled in the art can flexibly set the temperature according to factors such as the specific type of the cells to be cultured in practical application, and such adjustment and change of specific values of the ambient temperature in the cell culture section 1 do not deviate from the principle and scope of the present utility model, and should be limited to the protection scope of the present utility model.
In addition, it should be noted that, the environmental temperature of the sample buffer 3 is not limited to 4 degrees, and those skilled in the art may flexibly set the environmental temperature of the sample buffer 3 according to factors such as specific types of cells to be cultured in practical applications, for example, the environmental temperature of the sample buffer 3 may be set to 2 degrees, 3 degrees, 5 degrees or 6 degrees, and such adjustment and change of specific values of the environmental temperature in the sample buffer 3 do not deviate from the principle and scope of the present utility model, and should be limited to the protection scope of the present utility model. Of course, the ambient temperature in the sample buffer 3 is preferably set to 2 to 6 degrees, and most preferably set to 4 degrees.
In addition, the present utility model is not limited to the specific configuration of the first transporting means 4, as long as the cell sap which can be separated by the first transporting means 4 is transported from the cell sap separation region 2 to the cell culture region 1.
In addition, the specific configuration of the second transporting device 5 is not limited in the present utility model, as long as the cultured cell sample can be transported from the cell culture section 1 to the sample buffer section 3 by the second transporting device 5.
Preferably, as shown in fig. 1, the first conveying device 4 is a vertical mechanical arm.
Illustratively, the vertical mechanical arm is installed in the cell culture area 1 and is arranged corresponding to an opening 81 communicating the cell culture area 1 with the cell liquid division area 2, when the cell liquid after liquid division in the cell liquid division area 2 needs to be conveyed to the cell culture area 1, the opening 81 between the cell culture area 1 and the cell liquid division area 2 is opened by the electric door, the vertical mechanical arm ascends and penetrates through the opening 81, the sample box after liquid division is grasped, then the sample box is carried down into the cell culture area 1, and the opening 81 is closed by the electric door.
Illustratively, the vertical mechanical arm includes a fixed mount, and a vertical rail, a driving mechanism and a manipulator mounted on the fixed mount, the driving mechanism being capable of driving the manipulator to move up and down along the vertical rail, the manipulator being capable of gripping the sample cartridge.
It should be noted that, the driving mechanism may be configured as a motor and a gear and a rack engaged with each other, the motor is mounted on the manipulator and connected to the gear, the rack is mounted on the fixing frame and extends in a vertical direction, the motor can drive the gear to rotate, so that the gear moves up and down along the rack, and simultaneously drives the motor and the manipulator to move up and down along the vertical track, or the gear and the rack engaged with each other may be replaced by a screw and a nut, or the driving mechanism may be configured as a hydraulic driving mechanism, etc., and the specific type of adjustment and change of the driving mechanism do not deviate from the principle and scope of the present utility model, and should be limited in the scope of protection of the present utility model.
Preferably, as shown in fig. 1, the second conveying device 5 is a six-axis robot.
Wherein the six-axis robot is installed in the sample buffer zone 3 and is disposed near an opening 81 communicating the cell culture zone 1 with the sample buffer zone 3.
When the cell samples which are cultured in the cell culture zone 1 are required to be conveyed into the sample buffer zone 3, the electric door opens the opening 81 between the cell culture zone 1 and the sample buffer zone 3, the six-axis robot stretches into the cell culture zone 1 through the opening 81, and the cultured cell samples are conveyed into the sample buffer zone 3 for storage.
Preferably, as shown in FIG. 2, the cell dispensing device 6 of the present utility model comprises a tube removal and uncapping mechanism 61, a dispensing mechanism 62, and a fixing base 63.
The tube moving and uncapping mechanism 61 can clamp a sample tube containing cell stock solution and transfer the sample tube onto the fixing seat 63, three fixing holes 631 are formed in the fixing seat 63, the sample tube is inserted into the fixing holes 631 to prevent the sample tube from rotating, the tube moving and uncapping mechanism 61 clamps a top cover of the sample tube and drives the top cover to rotate so as to open the sample tube, and then the cell stock solution in the sample tube is completely sucked out and distributed into a plurality of culture tubes by the liquid distributing mechanism 62.
Those skilled in the art will appreciate that while some embodiments described herein include some features but not others included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the present application and form different embodiments. For example, in the claims of the present application, any of the claimed embodiments may be used in any combination.
Thus far, the technical solution of the present utility model has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present utility model is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present utility model, and such modifications and substitutions will fall within the scope of the present utility model.
Claims (10)
1. A cell culture apparatus, comprising:
a cell culture section for performing a cell culture work;
the cell liquid separation area is used for carrying out liquid separation treatment on the cell stock solution and is communicated with the cell culture area;
a first conveying device for conveying the cell sap separated from the cell sap separation region to the cell culture region;
a sample buffer zone for storing a cultured cell sample, the sample buffer zone being in communication with the cell culture zone; and
and the second conveying device is used for conveying the cultured cell sample from the cell culture area to the sample buffer area.
2. The cell culture apparatus of claim 1, wherein the cell-separating region is located above the cell-culturing region, and wherein a bottom of the cell-separating region is in communication with a top of the cell-culturing region.
3. The cell culture apparatus of claim 2, wherein the first transport device is a vertical robotic arm.
4. The cell culture apparatus of claim 1 or 2, wherein the sample buffer zone is located above the cell culture zone, and wherein the bottom of the sample buffer zone is in communication with the top of the cell culture zone.
5. The cell culture apparatus of claim 4, wherein the second transport device is a six-axis robot.
6. The cell culture apparatus of claim 5, wherein the six-axis robot is mounted within the sample buffer.
7. The cell culture apparatus of claim 1, wherein the ambient temperature in the sample buffer is 2 to 6 degrees.
8. The cell culture apparatus of claim 7, wherein the ambient temperature in the sample buffer is 4 degrees.
9. The cell culture apparatus of claim 1, wherein the cell-separating region and the sample buffer region are respectively located at left and right sides of the cell culture region, the left and right sides of the cell culture region being respectively in communication with the cell-separating region and the sample buffer region.
10. The cell culture apparatus of claim 1, wherein the cell-separating region and the sample buffer region are respectively located on front and rear sides of the cell culture region, the front and rear sides of the cell culture region being respectively communicated with the cell-separating region and the sample buffer region.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223012600.9U CN219194989U (en) | 2022-11-11 | 2022-11-11 | Cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223012600.9U CN219194989U (en) | 2022-11-11 | 2022-11-11 | Cell culture apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN219194989U true CN219194989U (en) | 2023-06-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223012600.9U Active CN219194989U (en) | 2022-11-11 | 2022-11-11 | Cell culture apparatus |
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| CN (1) | CN219194989U (en) |
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- 2022-11-11 CN CN202223012600.9U patent/CN219194989U/en active Active
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