CN219194957U - Device for quickly classifying and picking multiple monoclonal colonies - Google Patents

Abstract

The utility model discloses a device for quickly classifying and picking a plurality of monoclonal colonies, which comprises a fungus picking workstation, an in-situ detection device and a computer end, wherein the fungus picking workstation and the in-situ detection device are respectively connected with the computer end through an electric signal path to realize information transmission and equipment control. The bacterial picking workstation shoots the culture through the camera unit, the computer end analyzes and positions the bacterial colony on the camera, then the culture is transferred to the microscopic detection device to carry out in-situ detection on the bacterial colony, the culture is placed on the objective table of the bacterial picking workstation again according to the camera position after detection, the computer end analyzes and processes in-situ detection information, and the bacterial colony classification result is sent to the bacterial picking workstation to pick the bacterial colony.

Description

Device for quickly classifying and picking multiple monoclonal colonies

Technical Field

The utility model relates to a device capable of quickly classifying and automatically picking a plurality of monoclonal colonies.

Background

Isolation and purification culture of bacteria is an important part of bacterial research, and with the rapid development of microbial research, researchers need to rapidly isolate and culture as many bacterial species as possible from organisms or surrounding environments for analytical research. As the separation work proceeds, more and more bacterial species are separated, and correspondingly, the difficulty of separating new and uncultured bacterial species is also greater, and the work efficiency is lower, mainly because: the strains of the same species are repeatedly selected and repeatedly identified, thereby wasting manpower, material resources and time. To break through the current situation, on one hand, the same strain needs to be identified to reduce the weight, and on the other hand, the low efficiency of artificial fungus picking needs to be improved.

The artificial fungus picking is to select the colony to be identified from the culture medium through manual operation and transfer the colony to a target container for purification, and the problems of low efficiency and large error exist.

The in-situ detection technology is a method capable of rapidly and accurately detecting monoclonal bacterial colonies, is more convenient and faster, has no destructiveness and invasiveness to detection samples, and can be used for carrying out nondestructive detection on bacterial colony states or internal structures in an in-situ state. Common in situ detection techniques are raman spectroscopy, near infrared spectroscopy, hyperspectral imaging techniques, and the like. The Raman spectrum technology has the advantages of marking or not and not damaging a sample, and molecular structure information in the aspects of molecular vibration and rotation contained in the colony is obtained by analyzing a scattering spectrum with different frequency from the incident light, so that researchers are helped to quickly classify the monoclonal colony, and the sampling efficiency is greatly improved. The near infrared spectrum technology is based on the spectrum technology to detect the species and the state of the bacterial colony, and the nucleic acid, the protein and other components of the microorganism in the bacterial colony can absorb near infrared light to generate different spectrum information, so that the near infrared spectrum information, the structural composition of the microorganism and the correlation detection on the microorganism number can be established. The hyperspectral imaging technology combines the characteristics of spectroscopy and imaging technology, can detect the spectral information and the image information to be detected simultaneously, and analyzes the colony detection information through the curve generated by the absorption spectrum.

There are already some examples of in situ detection techniques in the prior art that are applied in colony identification. For example, patent CN107586823a uses a portable raman detector to rapidly detect and identify carbapenem-sensitive bacteria or drug-resistant bacteria. Patent CN112098389B combines the space-limiting effect and raman fingerprint spectrum to realize rapid specific detection of listeria monocytogenes. However, the specific spectrum information of each strain is insufficient at present, and the spectrum information of the bacterial colonies in different growth stages and growth environments is also different, so that the in-situ identification of any strain cannot be realized, and the in-situ identification method of a specific strain is provided in the prior art.

In terms of fungus picking technology, in order to improve fungus picking efficiency, the patent CN217377872U and the patent CN109321429A provide devices capable of automatically picking fungus.

However, the prior art cannot realize the effect of automatically and efficiently picking different strains.

Disclosure of Invention

The utility model provides a device and a method capable of quickly classifying and automatically picking a plurality of monoclonal colonies, aiming at the defects of the prior art.

As a first aspect of the present utility model, there is provided an apparatus for rapid classification and picking of a plurality of monoclonal colonies, the apparatus comprising:

1) The fungus picking workstation comprises a horizontal box body, a vertical box body, a shooting unit, a fungus picking unit, a moving assembly, an objective table and a collecting plate, wherein the objective table, the collecting plate and the moving assembly are fixed on the horizontal box body, the objective table is detachably fixed, the horizontal box body is vertically and fixedly connected with the vertical box body, the shooting unit is fixed on the vertical box body, the fungus picking unit is fixed on the moving assembly and is positioned above the objective table, the moving assembly drives the fungus picking unit to move in the horizontal and vertical directions, the shooting unit is positioned above the objective table to photograph a sample on the objective table, the fungus picking unit comprises a pipetting suction module and a pipetting head arranged at the bottom of the pipetting suction module, and the sample picked by the fungus picking unit on the objective table is transferred into the collecting plate;

2) The in-situ detection device comprises a microscopic detector, an in-situ detection spectrometer and a laser, wherein the laser provides an excitation light source for the in-situ detection spectrometer, and transmits a light path to a sample point of the microscopic detector after being processed by the in-situ detection spectrometer, and the microscopic detector images to obtain in-situ detection information of the sample; and

3) And the bacteria picking unit, the moving assembly, the camera shooting unit, the in-situ detection spectrometer and the microscopic detection instrument are respectively connected with the computer end through an electric signal path, so that information transmission and equipment control are realized.

The device can realize rapid classification and automatic picking of a plurality of monoclonal colonies, and comprises the steps of placing a culture on a stage of a bacteria picking workstation, photographing the culture by a camera unit, analyzing and positioning the colonies by a computer end on the photograph, transferring the culture to a microscopic detector for in-situ detection, and then placing the culture on the stage of the bacteria picking workstation according to photographic position. The computer end analyzes and processes the in-situ detection information, and includes classifying and analyzing the in-situ detection information, selecting target colonies for each classified colony, then sending classification results including the number, coordinate information, belonging classification pile number and the like of the colonies to the bacteria picking workstation, and the bacteria picking unit of the bacteria picking workstation aims at the target colonies under the drive of the moving assembly to sequentially pick target colonies with different classification pile numbers and transfer the target colonies into the collecting plate, so that rapid classification and automatic picking of a plurality of monoclonal colonies are realized.

According to the utility model, the in-situ detection information is utilized to classify the bacterial colonies in the culture, only one bacterial colony is selected according to each classification result, the classifying and de-duplication method is convenient and quick, the sample to be detected is not destructive and invasive, and the automatic bacterial selection efficiency is greatly improved.

In a preferred embodiment, the device for rapid sorting and picking of a plurality of monoclonal colonies further comprises a pipetting head box fixed on the horizontal box body, wherein two or more pipetting heads are arranged in the pipetting head box, and the buckling structure is provided with an inverted L-shaped opening and leads out of the horizontal box body through a slope slideway. According to the preferred scheme, after the fungus drops are picked and placed each time, the movable assembly drives the fungus picking unit to enable the liquid transferring head to be inserted into the inverted L-shaped opening to be clamped, the movable assembly is lifted upwards, the liquid transferring head is separated and discharged out of the horizontal box body along the slideway, the movable assembly drives the fungus picking unit to be arranged above the liquid transferring head box, a new liquid transferring head is downwards arranged, another bacterial colony is picked, and automatic repeated fungus picking is achieved in a reciprocating mode.

In a preferred embodiment, the stage is provided with means for securing the culture dish, such as screws, by which the culture dish containing the culture can be secured to the stage against deflection or movement. The structure of the fixed culture dish can also be an elastic structure, and the culture dish is placed in the culture dish to deform the elastic structure to generate pressure so as to fix the culture dish.

In another preferred embodiment, the collection plate is a 96-well plate or 384-well plate.

In another preferred embodiment, the middle part of the objective table is hollowed out, and a bottom illumination unit positioned below the hollowed-out structure of the middle part is installed in the horizontal box body, and the bottom illumination unit can directly irradiate the bottom of the vessel filled with the culture. The illumination intensity can be improved by providing the light of the transmission vessel from the bottom by the bottom illumination unit, so that the photo of the sample is clearer, particularly, colonies with a certain depth in the culture medium can be more clearly imaged through the transmitted light, and the phenomenon that some rare colonies cannot be picked up is avoided.

In another preferred embodiment, the stage has a first positioning structure and the horizontal housing is provided with a second positioning structure matching the shape of the first positioning structure.

The utility model needs to move the culture sample back and forth between the bacteria picking workstation and the in-situ detection device, and the vessel containing the culture is usually a round culture dish, so that the culture sample is difficult to replace in the same position after moving. In order to quickly place the culture in the same position to increase the speed and accuracy of the picking, the vessel containing the culture may be fixed to the stage, together with the stage for transfer. However, even so, the operator needs to carefully recognize the mounting manner of the stage at the time of operation so as not to be erroneously mounted. The positioning structure enables the staff to immediately confirm the installation direction and quickly replace the object stage at the same position.

In another preferred embodiment, the aforementioned monoclonal colony rapid sorting and picking device, the displacement stage of the microscopic detector is provided with a third positioning structure matching the shape of the first positioning structure. The third positioning structure is arranged to facilitate the rapid calibration of the coordinates of the same bacterial colony in the bacterial picking workstation and the in-situ detection device from a physical angle.

In another preferred embodiment, the device for rapid classification and picking of monoclonal colonies further comprises a mechanical arm fixedly connected with the objective table, wherein the mechanical arm is connected with the computer end through an electric signal path, and the mechanical arm can automatically transfer the objective table between the bacteria picking workstation and the displacement table of the in-situ detection device under the control of the computer. The optimal scheme can realize the whole classification and full mechanization of the fungus picking process, and avoid time waste and operation errors caused by manual transfer.

The moving assembly of the present utility model may take any of the solutions known in the mechanical arts, which are readily implemented in the art. In a preferred embodiment of the utility model, the moving assembly comprises two longitudinal guide rails fixed on the horizontal box body in parallel, a transverse guide rail arranged on the two longitudinal guide rails in a sliding manner in a crossing manner, and a vertical guide rail arranged on the transverse guide rail in a vertical sliding manner, the fungus picking unit is arranged on the vertical guide rail in a sliding manner, and the transverse guide rail, the vertical guide rail and the fungus picking unit are connected with a driving assembly which is connected with a computer end. According to the scheme, the fungus picking unit slides on the vertical guide rail, the vertical guide rail slides on the transverse guide rail, and the transverse guide rail slides on the longitudinal guide rail comprehensively to realize the movement of the fungus picking unit at different positions.

In another preferred embodiment, the pipetting suction module comprises a suction cylinder and a suction rod positioned in the suction cylinder, a connector communicated with the interior is arranged at the bottom of the suction cylinder, the connector is connected with a pipetting head in a buckling manner, a sealing rubber plug is fixed at the bottom end of the suction rod, and the outer edge of the sealing rubber plug is in sealing contact with the suction cylinder. The suction rod moves up and down to generate pressure difference to drive the pipetting head to suck and discharge the content, so that the picking and transferring of the bacterial colony are realized.

In another preferred embodiment, the picking station further comprises a top illumination unit secured to the vertical housing above the stage.

In another preferred embodiment, the in situ detection spectrometer is a raman spectrometer, a near infrared spectrometer or a hyperspectral imager.

As a second aspect of the present utility model, there is provided a method for rapidly classifying and automatically picking monoclonal colonies, the method comprising:

s1, a camera of a fungus picking workstation shoots a culture, the coordinate information of the culture is identified through the photo, the origin of coordinates is calibrated, colony numbers are edited for colonies at different positions, and then each colony is obtained to be accurately positioned;

s2, placing the culture in an in-situ detection device, aligning the in-situ detection device with the origin of coordinates of the culture, and calibrating the coordinates of the bacteria picking workstation and the in-situ detection device;

s3, setting colony signal acquisition parameters of the in-situ detection device, acquiring in-situ detection information of each colony, and putting the culture back to the fungus picking workstation again;

s4, carrying out classification analysis on the in-situ detection information obtained in the S3, and sending detection and analysis results to a fungus picking workstation for colony selection;

s5, sequentially picking colonies with different classification stacking numbers by the fungus picking workstation, and transferring the colonies into a target container.

The culture of the present utility model may be any microbial culture including soil microorganisms, marine microorganisms, pathogenic microorganisms, intestinal microorganisms, ice layer microorganisms, food-borne microorganisms, engineered microorganisms, etc., and the source thereof is not limited.

The bacterial colony comprises different microorganism monoclonal bacterial colonies such as escherichia coli, bacillus subtilis, streptococcus casei and the like, and the species of the bacterial colony is not limited.

As a preferred embodiment, the culture is detected in the form of a petri dish carrying the culture.

As a preferred technical solution, the in-situ detection device adopts raman spectroscopy, near infrared spectroscopy or hyperspectral imaging. More preferably, the in situ detection device employs raman spectroscopy.

As a preferred embodiment, the classification analysis in S4 is a supervised classification analysis, an unsupervised cluster analysis, or a similarity calculation.

As a preferred embodiment, the detection and analysis results of S4 include, but are not limited to, information on colony culture, information on spectral coordinates of colonies, colony numbers, belonging classification stack numbers, etc.

The utility model has the beneficial effects that:

1. the in-situ detection technology is adopted to carry out nondestructive classification and identification on a plurality of bacterial colonies, one bacterial colony is selected from each type, so that the efficiency of obtaining different bacterial colonies is improved, repeated selection is avoided, the identification method is short in time consumption and labor is saved;

2. the fungus picking workstation replaces manual fungus picking, can continuously and rapidly pick fungus, and has high fungus picking efficiency;

3. the bacterial picking workstation is aligned with colony coordinates in-situ detection, so that consistency of the identified bacterial strain and the picked bacterial strain is ensured, and accuracy is high.

Drawings

FIG. 1 is an overall schematic of a picking station;

FIG. 2 is a right side view of the picking station of FIG. 1;

FIG. 3 is a partial construction diagram of the fungus picking unit;

FIG. 4 is a partial schematic view of a snap-fit arrangement;

FIG. 5 is a schematic diagram of an in situ detection device;

FIG. 6 is a representation of the clustering results of a cluster analysis using the method of the present utility model.

Reference numeral, 1-horizontal box, 2-vertical box, 3-camera unit, 4-picking unit, 41-pipetting head, 5-objective table, 6-collecting plate, 7-pipetting head box, 8-bayonet structure, 81-inverted L-shaped opening, 9-slideway, 10-longitudinal rail, 11-transverse rail, 12-vertical rail, 13-microscopic detector, 131-displacement table, 14-laser and 15-in-situ detection spectrometer.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below: it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments, and that all other embodiments obtained by a person skilled in the art without making creative efforts based on the embodiments in the present utility model shall fall within the protection scope of the present utility model.

In the description of the present utility model, it should be understood that the terms "longitudinal," "transverse," "vertical," "upper," "lower," "front," "rear," "left," "right," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present utility model and simplify the description, and do not indicate or imply that the devices or elements being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.

Example 1

Referring to fig. 1-5, an apparatus for rapid classification and picking of monoclonal colonies, comprising:

the fungus picking workstation comprises a horizontal box body 1, a vertical box body 2, a camera shooting unit 3, a fungus picking unit 4, a moving assembly, an object stage 5 and a collecting plate 6, wherein the object stage 5, the collecting plate 6 and the moving assembly are fixed on the horizontal box body 1, the object stage 5 is detachably fixed, the horizontal box body 1 is vertically and fixedly connected with the vertical box body 2, the camera shooting unit 3 is fixed on the vertical box body 2, the fungus picking unit 4 is fixed on the moving assembly and is positioned above the object stage 5, the moving assembly drives the fungus picking unit 4 to move in horizontal and vertical directions, the camera shooting unit 3 is positioned above the object stage 5 to shoot a sample on the object stage 5, the fungus picking unit 4 comprises a liquid transferring suction module and a liquid transferring head 41 arranged at the bottom of the fungus picking unit 4, and the sample on the object stage 5 is picked up and transferred into the collecting plate 6; the movable assembly comprises two longitudinal guide rails 10 which are fixed on the horizontal box body 1 in parallel, a transverse guide rail 11 which is arranged on the two longitudinal guide rails 10 in a sliding way in a crossing way, and a vertical guide rail 12 which is arranged on the transverse guide rail 11 in a vertical sliding way, the fungus picking unit 4 is arranged on the vertical guide rail 12 in a sliding way, the transverse guide rail 11, the vertical guide rail 12 and the fungus picking unit 4 are all connected with a driving assembly, and the driving assembly is connected with a computer end;

the in-situ detection device is shown in fig. 5, and comprises a microscopic detector 13, an in-situ detection spectrometer 15 (raman spectrometer) and a laser 14, wherein the laser 14 provides an excitation light source for the in-situ detection spectrometer 15, and transmits a light path to a sample point of the microscopic detector 13 after being processed by the in-situ detection spectrometer 15, and the microscopic detector 13 detects in-situ detection information of the sample;

and the bacteria picking unit 4, the moving assembly, the camera unit 3, the in-situ detection spectrometer 15 and the microscopic detector 13 are respectively connected with the computer end through an electric signal path, so that information transmission and equipment control are realized.

Example 2

Referring to fig. 1-5, a monoclonal colony rapid classifying and picking device, based on example 1, further comprises: the pipetting head box 7 and the buckle structure 8 of fixing on the horizontal box 1, pipetting head 41 two or more are equipped with in the pipetting head box 7, buckle structure 8 has an inverted L type opening 81 to outside leading to the horizontal box 1 through a slope slide 9, the collecting plate is 96 orifice plates, and the middle part fretwork of objective table 5 installs the bottom lighting unit that is located middle part fretwork structure below in the horizontal box 1, the bottom lighting unit can direct irradiation to the household utensils bottom that is equipped with the culture. The bottom lighting unit can improve photo definition and colony information accuracy, and the arrangement of the pipetting head box and the buckle structure enables the device to continuously pick bacteria for many times.

One side of the objective table 5 is provided with a penetrating screw, and the culture dish can be fixed by tightening the screw. The outer edge of the objective table 5 has a first positioning structure, such as a triangle or a semicircular convex structure, the horizontal box 1 is provided with a second positioning structure matched with the shape of the first positioning structure, such as a groove matched with the triangle or the semicircular convex structure, and the displacement table 131 of the micro-detector 13 is provided with a third positioning structure matched with the shape of the first positioning structure. In addition, the mechanical arm can replace the function of the positioning structure, the mechanical arm is fixedly connected with the objective table 5 and is connected with the computer end through an electric signal path, the mechanical arm can automatically transfer the objective table 5 between the bacteria picking workstation and the displacement table of the in-situ detection device under the control of the computer to realize the directional transfer of the objective table 5, and the effect of rapid preparation and transfer at the same position can also be realized.

The pipetting suction module comprises a suction cylinder and a suction rod positioned in the suction cylinder, a connector communicated with the inside is arranged at the bottom of the suction cylinder, the connector is connected with a pipetting head 41 in a buckling manner, a sealing rubber plug is fixed at the bottom end of the suction rod, and the outer edge of the sealing rubber plug is in sealing contact with the suction cylinder. The suction rod moves up and down to generate pressure difference to drive the pipetting head to suck and discharge the content, so that the picking and transferring of the bacterial colony are realized.

Example 3

In connection with the apparatus of embodiment 1 or 2, a method is provided for rapid classification and automatic picking of monoclonal colonies, the method comprising:

s1, fixing a culture dish containing colony cultures on an objective table of a bacteria picking workstation, turning on a bottom lighting unit, photographing the cultures by a camera of the bacteria picking workstation, transmitting photo information to a computer end, identifying culture coordinate information by the photos, calibrating a coordinate origin, editing colony numbers for colonies at different positions, and further obtaining accurate positioning of each colony;

s2, automatically or manually transferring the object stage together with the culture onto a displacement table of a microscopic detector, adjusting the instrument, opening a Raman spectrometer and a laser, setting detection conditions of the Raman spectrometer, such as 600 gratings, a laser wavelength of 532nm, an excitation time of 3S and an excitation energy of 5mW, carrying out displacement calibration and intensity calibration on the Raman spectrometer, aligning a laser cursor of the Raman spectrometer with a culture dish coordinate origin, aligning an in-situ detection device with the culture coordinate origin, calibrating coordinates of a bacteria picking workstation and the in-situ detection device, and ensuring that colony positions between the bacteria picking workstation and the in-situ detection device are consistent;

s3, setting colony signal acquisition parameters of a Raman spectrometer, for example, setting spectrum acquisition step length: the steps of the X axis, the Y axis and the Z axis are respectively 2 mu m, 2 mu m and 0.5 mu m, a laser cursor is positioned to a colony to be detected, in-situ detection information of each colony is collected, and cultures are manually or automatically put back to a fungus picking workstation at the same position;

s4, the computer end carries out classification analysis on the in-situ detection information obtained in the S3, for example, a clustering method of unsupervised analysis is used for detecting a Raman spectrum clustering result of the detected colony, raman Hooke intP Raman preprocessing software is used for processing Raman spectrum data of the colony in batches, and preprocessing parameter information comprises: filtering the Raman data of the colony based on Savitzky-Golay convolution smoothing, wherein the window width is 5 spectrum pixel points, and 3-order polynomial fitting is adopted; removing a Raman spectrum background signal by using an airPLS (adaptive iteration re-weighting punishment least square) algorithm, wherein lambda=100 and the maximum iteration number ItermaxAirPls=15; and (5) carrying out normalization treatment on colony Raman data by using Min-Max. Further, performing nonlinear dimension reduction analysis on colony Raman spectrum data by using t-SNE; further, performing cluster analysis on the Raman spectrum data of the colony subjected to dimension reduction by using spectral clustering, returning a cluster heap number to which a colony number belongs, for example, a cluster result shown in FIG. 6, and sending the detection and analysis result to a bacteria picking workstation for colony selection;

s5, the fungus picking workstation drives the moving assembly to drive the fungus picking unit to sequentially pick the colonies with different classification pile numbers identified by the in-situ detection device from the culture dish and transfer the colonies to the collecting plate, for example, a 96-hole plate, after each fungus drop transfer, the fungus picking unit moves to the clamping structure and stretches into the inverted L-shaped opening to enable the liquid transferring head to be clamped, the fungus picking unit lifts up, the liquid transferring head falls into the opening structure and slides out of the horizontal box body along a slide way with a slope, the fungus picking unit moves to the upper side of the liquid transferring head box to align with the new liquid transferring head, and the liquid transferring head is reloaded downwards and then transferred to the next target colony to pick the fungus.

According to the method, colonies with different types and characteristics in the culture dish can be continuously picked through one-time photographing and one-time in-situ detection, and quick classification and picking of monoclonal colonies are realized.

Claims (9)

1. An apparatus for rapid classification and picking of a plurality of monoclonal colonies, the apparatus comprising:

the device comprises a fungus picking workstation, wherein the fungus picking workstation comprises a horizontal box body (1), a vertical box body (2), a camera shooting unit (3), a fungus picking unit (4), a moving assembly, an object table (5) and a collecting plate (6), the object table (5), the collecting plate (6) and the moving assembly are fixed on the horizontal box body (1), the object table (5) is fixed in a detachable mode, the horizontal box body (1) is vertically and fixedly connected with the vertical box body (2), the camera shooting unit (3) is fixed on the vertical box body (2), the fungus picking unit (4) is fixed on the moving assembly and is located above the object table (5), the moving assembly drives the fungus picking unit (4) to move in the horizontal and vertical directions, the camera shooting unit (3) is located above the object table (5), the fungus picking unit (4) comprises a liquid transferring suction module and a liquid transferring head (41) arranged at the bottom of the fungus picking unit (4), and the sample on the fungus picking unit (5) is transferred into the collecting plate (6);

2) The in-situ detection device comprises a microscopic detector (13), an in-situ detection spectrometer (15) and a laser (14), wherein the laser (14) provides an excitation light source for the in-situ detection spectrometer (15), and transmits a light path to a sample point of the microscopic detector (13) after being processed by the in-situ detection spectrometer (15), and the microscopic detector (13) images to obtain in-situ detection information of the sample; and

3) And the bacteria picking unit (4), the moving assembly, the camera unit (3), the in-situ detection spectrometer (15) and the microscopic detector (13) are respectively connected with the computer end through an electric signal path, so that information transmission and equipment control are realized.

2. The device for rapid sorting and picking of a plurality of monoclonal colonies according to claim 1, further comprising a pipetting head box (7) fixed on the horizontal box (1) and a snap-on structure (8), wherein two or more pipetting heads (41) are mounted in the pipetting head box (7), and the snap-on structure (8) has an inverted-L-shaped opening (81) and is led out of the horizontal box (1) through a slide (9) with a slope.

3. The device for rapid sorting and picking of a plurality of monoclonal colonies according to claim 1 or 2, wherein the stage (5) has a hollow central portion, and a bottom illumination unit located below the hollow central portion is installed in the horizontal case (1), and the bottom illumination unit can directly irradiate the bottom of the vessel containing the culture.

4. Device for the rapid sorting and picking of a plurality of monoclonal colonies according to claim 1 or 2, characterized in that the stage (5) has a first positioning structure, the horizontal box (1) is provided with a second positioning structure matching the shape of the first positioning structure, and the stage (5) is provided with a structure for fixing the culture dish.

5. The device for rapid sorting and picking of multiple monoclonal colonies according to claim 4, wherein the displacement stage (131) of the microscopic detector (13) is provided with a third positioning structure matching the shape of the first positioning structure.

6. The device for rapid sorting and picking up of a plurality of monoclonal colonies according to claim 1 or 2, further comprising a mechanical arm fixedly connected to the stage (5), the mechanical arm being connected to the computer end by means of an electrical signal path, the mechanical arm being capable of automatically transferring the stage (5) between the picking station and the in situ detection device under control of the computer.

7. Device for the rapid sorting and picking of a plurality of monoclonal colonies according to claim 1 or 2, characterized in that the moving assembly comprises two longitudinal rails (10) fixed in parallel on a horizontal box (1), a transverse rail (11) slidingly mounted in a transversal manner on the two longitudinal rails (10), and a vertical rail (12) slidingly mounted vertically on the transverse rail (11), the picking unit (4) being slidingly mounted on the vertical rail (12), the transverse rail (11), the vertical rail (12) and the picking unit (4) being connected to a driving assembly, which is in turn connected to a computer terminal.

8. The device for quickly classifying and picking up a plurality of monoclonal colonies according to claim 1 or 2, wherein the pipetting suction module comprises a suction cylinder and a suction rod positioned in the suction cylinder, a connector communicated with the interior is arranged at the bottom of the suction cylinder, the connector is in snap connection with a pipetting head (41), a sealing rubber plug is fixed at the bottom end of the suction rod, and the outer edge of the sealing rubber plug is in sealing contact with the suction cylinder.

9. Device for rapid sorting and picking of a plurality of monoclonal colonies according to claim 1 or 2, characterized in that the in situ detection spectrometer (15) is a raman spectrometer, a near infrared spectrometer or a hyperspectral imager.

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