CN218382526U - Four-dimensional electrophoresis apparatus - Google Patents
Four-dimensional electrophoresis apparatus Download PDFInfo
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- CN218382526U CN218382526U CN202222425051.1U CN202222425051U CN218382526U CN 218382526 U CN218382526 U CN 218382526U CN 202222425051 U CN202222425051 U CN 202222425051U CN 218382526 U CN218382526 U CN 218382526U
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Abstract
The utility model provides a four-dimensional electrophoresis apparatus, include: the electrophoresis device comprises an electrophoresis tank, electrode plates arranged at two ends of the electrophoresis tank, an electrophoresis carrier arranged in the electrophoresis tank, a first permanent magnet and a second permanent magnet which are oppositely arranged at two sides of the electrophoresis carrier, and a data acquisition device; the data acquisition device comprises a single chip microcomputer, a data acquisition head assembly and a driving motor, wherein the data acquisition head assembly is arranged between the first permanent magnet and the second permanent magnet, the driving motor is connected with the data acquisition head assembly and can control the data acquisition head assembly to move back and forth along the axial direction of the electrophoresis carrier, and the single chip microcomputer is electrically connected with the data acquisition head assembly and the driving motor. The utility model discloses an increase a set of data acquisition device that can follow electrophoresis carrier axial displacement on three-dimensional electrophoresis device, come to carry out real-time supervision to the charged particle of electrophoresis in-process to obtain four-dimensional image, so that carry out detailed analysis to the electrophoresis condition of electrophoresis in-process.
Description
Technical Field
The utility model relates to an experimental device field especially relates to a four-dimensional electrophoresis apparatus.
Background
Electrophoresis technology is an important analysis means which is indispensable to molecular biology research, and an electrophoresis apparatus is an important equipment tool for realizing the electrophoresis technology. Electrophoresis is the movement of charged particles in an electric field, and different substances have different movement speeds in the electric field due to different charged amounts and molecular weights, so that according to the characteristics, qualitative or quantitative analysis can be performed on different substances by using an electrophoresis method, or analysis of components of a certain mixture or extraction and preparation of single components can be performed, which has extremely important significance in clinical examination or experimental research.
Prior art CN202220972396.6 discloses a multi-dimensional electrophoresis apparatus comprising: the electrophoresis tank, the electrode plate, the electrophoresis carrier and the permanent magnet; the electrode plates are arranged at two ends of the electrophoresis tank and are respectively connected with the anode and the cathode of a power supply, the electrophoresis carrier is arranged in the electrophoresis tank, and the plane of the electrophoresis carrier is vertical to the plane of the electrode plates; the permanent magnet comprises a first permanent magnet and a second permanent magnet, the first permanent magnet and the second permanent magnet are oppositely arranged on two sides of the electrophoresis tank, close to the sample adding end, of the electrophoresis carrier, and the N pole of the first permanent magnet and the S pole of the second permanent magnet correspond to form a strong magnetic field perpendicular to the electric field.
The utility model discloses a although expand traditional one-dimensional electrophoresis for three-dimensional electrophoresis, expanded electrophoretic analysis's range of application, however, this utility model can't carry out real-time scanning to the data of electrophoresis in-process and gather, only can obtain final electrophoresis result, and in the electrophoresis of chronic disease detects, chronic disease is because long, probably carries out the mark change to protein, and can't obtain effectual result.
SUMMERY OF THE UTILITY MODEL
In view of this, the present invention provides a four-dimensional electrophoresis apparatus, which adds a set of data acquisition devices capable of moving axially along the electrophoresis carrier on a three-dimensional electrophoresis apparatus to monitor the charged particles in the electrophoresis process in real time, so as to obtain a four-dimensional image, thereby facilitating the detailed analysis of the whole situation in the electrophoresis process.
The utility model provides a technical scheme that its technical problem adopted is:
there is provided a four-dimensional electrophoresis apparatus comprising: the electrophoresis tank, electrode plates arranged at two ends of the electrophoresis tank, an electrophoresis carrier arranged in the electrophoresis tank, a first permanent magnet and a second permanent magnet which are oppositely arranged at two sides of the electrophoresis carrier, wherein the electrophoresis carrier is vertical to the plane of the electrode plates; the first permanent magnet and the second permanent magnet are arranged at the sample adding end of the electrophoresis carrier, and a strong magnetic field vertical to the electric field is formed between the first permanent magnet and the second permanent magnet;
the device also comprises a data acquisition device; the data acquisition device comprises a single chip microcomputer, a data acquisition head assembly and a driving motor, the data acquisition head assembly is arranged between a first permanent magnet and a second permanent magnet, the driving motor is connected with the data acquisition head assembly and can control the data acquisition head assembly to move back and forth along the axial direction of the electrophoresis carrier, and the single chip microcomputer is electrically connected with the data acquisition head assembly and the driving motor.
It should be noted that the data acquisition head assembly may adopt a device composed of an existing optical microscope lens and a light source; the single chip microcomputer controls the driving motor to control the motion of the data acquisition head assembly; meanwhile, the data acquisition head assembly sends the information acquired in real time to the singlechip through a cable for processing; real-time data in the process of dynamically acquiring and separating by real-time scanning are processed by a single chip microcomputer to construct a four-dimensional image; the final result of electrophoresis has a four-dimensional structure image, so that the separation precision of charged ions is improved; the magnetic attraction characteristic of the strong magnetic field is matched with the three-dimensional carrier to obtain the four-dimensional electrophoresis resolution capability of the separated macromolecules with the magnetic characteristic, so that the four-dimensional electrophoresis (space X, Y, Z three-dimensional and time dimension) is realized.
Preferably, the data collection head assembly comprises a bar light source and a light sensitive element, the bar light source is horizontally arranged between the electrophoresis carrier and the first permanent magnet and is axially vertical to the electrophoresis carrier, and the light sensitive element is arranged between the electrophoresis carrier and the second permanent magnet relative to the bar light source.
It should be noted that, because different molecules have different absorption degrees to light with different wavelengths, and different concentrations of the same molecule have different absorption degrees to the same light, after the light irradiates the sample in the carrier, the light can be irradiated on the light sensitive element through partial light absorbed by the molecules, the light sensitive element generates a corresponding electric signal and sends the electric signal to the single chip microcomputer, four-dimensional image information is formed through the processing of the single chip microcomputer, and finally, a four-dimensional image of the electrophoresis process is displayed on a computer, so that the observation and comparison of workers are facilitated.
Preferably, the strip-shaped light source is a light source obtained by homogenizing a series of LED light sources or halogen lamps.
Preferably, the light sensor is formed using a multi-channel multi-spectral sensor in a strip arrangement.
It should be noted that the spectrum sensor may be an AS7341 spectrum sensor, and the AS7341 spectrum sensor is a ll channel spectrometer, and is particularly suitable for spectrum identification and color matching applications in mobile devices.
Preferably, the driving motor is a stepping motor or a dc servo motor.
It should be noted that, by using a stepping motor or a dc servo motor, the traveling speed of the data collection head assembly can be controlled more accurately, so that the data collection head assembly can detect the electrophoresis process more accurately.
Preferably, the system also comprises a PC, wherein the PC is connected with the singlechip and can receive signals sent by the singlechip for processing.
It should be noted that, according to the electrophoresis information collected by the collecting head assembly, the electrophoresis information is processed by the single chip and sent to the PC, a four-dimensional image is constructed by processing the electrophoresis information by the PC, and the new information can be found by comparing the four-dimensional image with known data or standard data.
The strong magnetic field is distributed in the front half part of the electrophoresis carrier, and the permanent magnet occupies 1/3-4/5 of the length of the electrophoresis carrier relative to the direction of the electric field force.
The utility model has the advantages that:
the utility model provides a pair of four-dimensional electrophoresis apparatus through the collection system who increases a set of dynamic scanning real-time data on three-dimensional electrophoresis device, comes to carry out real-time supervision to the charged particle of electrophoresis in-process to send the information of gathering in real time to the singlechip through the cable and handle formation four-dimensional image information, so that carry out detailed analysis to the whole condition of electrophoresis in-process.
It should be noted that the present application is particularly suitable for the study of chronic diseases; chronic diseases have long time, and can label and change proteins, and the separation technology can separate the markers, so that the change of particles from beginning to end can be observed, and the process of pathological changes can be known conveniently.
Drawings
Fig. 1 is a left perspective view of a four-dimensional electrophoresis apparatus according to embodiment 1 of the present invention.
Fig. 2 is a right perspective view of a four-dimensional electrophoresis apparatus according to embodiment 1 of the present invention.
Fig. 3 is a cross-sectional view of a four-dimensional electrophoresis apparatus according to embodiment 1 of the present invention.
Fig. 4 is a schematic diagram of a four-dimensional electrophoresis apparatus according to embodiment 1 of the present invention.
Fig. 5 is a schematic perspective view of a driving motor and a data collecting head assembly according to embodiment 1 of the present invention.
Fig. 6 is a schematic perspective view of a four-dimensional electrophoresis apparatus according to embodiment 2 of the present invention.
Fig. 7 is a schematic diagram of a four-dimensional electrophoresis apparatus according to embodiment 2 of the present invention.
In the figure: 1. an electrophoresis tank; 2. an electrode sheet; 3. a first permanent magnet; 4. a second permanent magnet; 5. an electrophoresis carrier; 6. a data acquisition device; 61. a single chip microcomputer; 62. a data acquisition head assembly; 621. a strip light source; 622. a light sensitive element; 63. a drive motor; 7. a PC machine.
It should be noted that the drawings and the description are not intended to limit the scope of the inventive concept in any way, but rather to illustrate the inventive concept by those skilled in the art with reference to specific embodiments.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
As shown in fig. 1 to 5, a four-dimensional electrophoresis apparatus includes: the electrophoresis tank comprises an electrophoresis tank 1, electrode plates 2 arranged at two ends of the electrophoresis tank 1, an electrophoresis carrier 5 arranged in the electrophoresis tank 1, and a first permanent magnet 3 and a second permanent magnet 4 which are oppositely arranged at two sides of the electrophoresis carrier 5, wherein the electrophoresis carrier 5 is vertical to the plane of the electrode plates 2; the first permanent magnet 3 and the second permanent magnet 4 are arranged at the sample adding end of the electrophoresis carrier 5, and a strong magnetic field vertical to the electric field is formed between the first permanent magnet 3 and the second permanent magnet 4;
also comprises a data acquisition device 6; the data acquisition device 6 comprises a single chip microcomputer 61, a data acquisition head assembly 62 and a driving motor 63, the data acquisition head assembly 62 is arranged between the first permanent magnet 3 and the second permanent magnet 4, the driving motor 63 is connected with the data acquisition head assembly 62 and can control the data acquisition head assembly 62 to axially move back and forth along the electrophoresis carrier 5, and the single chip microcomputer 61 is electrically connected with the data acquisition head assembly 62 and the driving motor 63.
The data collecting head assembly 62 comprises a bar-shaped light source 621 and a light sensitive element 622, the bar-shaped light source 621 is horizontally arranged between the electrophoresis carrier 5 and the first permanent magnet 3 and is axially vertical to the electrophoresis carrier 5, and the light sensitive element 622 is arranged between the electrophoresis carrier 5 and the second permanent magnet 4 relative to the bar-shaped light source 621.
The bar light sources 621 are series connected LED light sources.
The photo sensor 622 is formed using a multi-channel multi-spectral sensor in a strip arrangement.
The driving motor 63 is a stepping motor.
The strong magnetic field is distributed in the front half part of the electrophoresis carrier 5, and the permanent magnet occupies 1/3 of the length of the electrophoresis carrier 5 relative to the direction of the force of the electric field.
The working principle and the using method of the four-dimensional electrophoresis apparatus of the embodiment are as follows:
the embodiment provides a four-dimensional electrophoresis apparatus, through increase a set of dynamic scanning real-time data's collection system on three-dimensional electrophoresis device, come to carry out real-time supervision to the charged particle of electrophoresis in-process to send the information of gathering in real time to singlechip 61 through the cable and handle and form four-dimensional image information, so that carry out detailed analysis to the whole condition of electrophoresis in-process.
When the electrophoresis device is used, experimental raw materials are placed on an electrophoresis carrier 5 (a thicker gel carrier), a first permanent magnet 3 and a second permanent magnet 4 are respectively arranged on the upper side and the lower side of the electrophoresis carrier 5, the S pole of the first permanent magnet 3 is oppositely arranged with the N pole of the second permanent magnet 4, a generated magnetic field is perpendicular to the plane of the electrophoresis carrier 5, the first permanent magnet 3 and the second permanent magnet 4 are arranged close to the front half part of the electrophoresis carrier 5, then a power supply of an electrode plate 2 is switched on to start electrophoresis analysis, and meanwhile, a data acquisition device 6 starts to operate; when the experimental raw material starts the electrophoretic analysis under the action of the electric field force and the magnetic field force, the single chip microcomputer 61 controls the driving motor 63 to start operating, the strip-shaped light source 621 is controlled to be turned on, the single chip microcomputer 61 receives and processes information detected by the spectrum sensor until the electrophoretic analysis is completed, the four-dimensional image information stored in the single chip microcomputer 61 is taken out by a worker, comparison is carried out according to known data or standard data, the difference can be found, and qualitative or quantitative analysis on different substances in the experimental raw material is completed.
Example 2
As shown in fig. 6 and 7, the four-dimensional electrophoresis apparatus of the present embodiment includes: the electrophoresis tank comprises an electrophoresis tank 1, electrode plates 2 arranged at two ends of the electrophoresis tank 1, an electrophoresis carrier 5 arranged in the electrophoresis tank 1, and a first permanent magnet 3 and a second permanent magnet 4 which are oppositely arranged at two sides of the electrophoresis carrier 5, wherein the electrophoresis carrier 5 is vertical to the plane of the electrode plates 2; the first permanent magnet 3 and the second permanent magnet 4 are arranged at the sample adding end of the electrophoresis carrier 5, and a strong magnetic field vertical to the electric field is formed between the first permanent magnet 3 and the second permanent magnet 4;
also comprises a data acquisition device 6; the data acquisition device 6 comprises a single chip microcomputer 61, a data acquisition head assembly 62 and a driving motor 63, the data acquisition head assembly 62 is arranged between the first permanent magnet 3 and the second permanent magnet 4, the driving motor 63 is connected with the data acquisition head assembly 62 and can control the data acquisition head assembly 62 to axially move back and forth along the electrophoresis carrier 5, and the single chip microcomputer 61 is electrically connected with the data acquisition head assembly 62 and the driving motor 63.
The data collecting head assembly 62 comprises a bar-shaped light source 621 and a light sensitive element 622, the bar-shaped light source 621 is horizontally arranged between the electrophoresis carrier 5 and the first permanent magnet 3 and is axially vertical to the electrophoresis carrier 5, and the light sensitive element 622 is arranged between the electrophoresis carrier 5 and the second permanent magnet 4 relative to the bar-shaped light source 621.
The strip light source 621 is a light source obtained by performing light equalization processing on a halogen lamp.
The photo sensor 622 is formed using a multi-channel multi-spectral sensor in a strip arrangement.
The driving motor 63 is a dc servo motor.
The device is characterized by further comprising a PC (personal computer) 7, wherein the PC 7 is connected with the single chip microcomputer 61 and can receive signals sent by the single chip microcomputer 61 and process the signals.
The strong magnetic field is distributed in the front half part of the electrophoresis carrier 5, and the permanent magnet occupies 4/5 of the length of the electrophoresis carrier 5 relative to the direction of the force of the electric field.
Compared with the first embodiment:
according to the four-dimensional electrophoresis apparatus provided by the embodiment, the data of the single chip microcomputer 61 is processed in real time by connecting the PC 7 to the single chip microcomputer 61, so that a worker can observe the change of an electrophoresis process in real time, and the working efficiency is improved; and by prolonging the distribution range of the magnetic field, the running path of the particles in the magnetic field is increased, so that the particles are more dispersed under the action of the magnetic force, the mutual influence among the particles is further reduced, and the electrophoresis analysis result is more accurate.
Finally, it is to be noted that: the above description is only the preferred embodiment of the present invention, which is only used to illustrate the technical solution of the present invention, and is not used to limit the protection scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "upper surface", "lower surface", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and may include, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Claims (7)
1. A four-dimensional electrophoresis apparatus comprising: the electrophoresis tank comprises an electrophoresis tank (1), electrode plates (2) arranged at two ends of the electrophoresis tank (1), an electrophoresis carrier (5) arranged in the electrophoresis tank (1), and a first permanent magnet (3) and a second permanent magnet (4) which are oppositely arranged at two sides of the electrophoresis carrier (5), wherein the electrophoresis carrier (5) is vertical to the plane of the electrode plates (2); the first permanent magnet (3) and the second permanent magnet (4) are arranged at the sample adding end of the electrophoresis carrier (5), and a strong magnetic field vertical to the electric field is formed between the first permanent magnet (3) and the second permanent magnet (4); the method is characterized in that:
also comprises a data acquisition device (6); the data acquisition device (6) comprises a single chip microcomputer (61), a data acquisition head assembly (62) and a driving motor (63), the data acquisition head assembly (62) is arranged between the first permanent magnet (3) and the second permanent magnet (4), the driving motor (63) is connected with the data acquisition head assembly (62) and can control the data acquisition head assembly (62) to axially move back and forth along the electrophoresis carrier (5), and the single chip microcomputer (61) is electrically connected with the data acquisition head assembly (62) and the driving motor (63).
2. The four-dimensional electrophoresis apparatus of claim 1 wherein: the data acquisition head assembly (62) comprises a bar-shaped light source (621) and a light sensitive element (622), the bar-shaped light source (621) is horizontally arranged between the electrophoresis carrier (5) and the first permanent magnet (3) and is axially vertical to the electrophoresis carrier (5), and the light sensitive element (622) is arranged between the electrophoresis carrier (5) and the second permanent magnet (4) relative to the bar-shaped light source (621).
3. The four-dimensional electrophoresis apparatus of claim 2 wherein: the strip-shaped light source (621) is a light source obtained by carrying out light homogenizing treatment on LED light sources or halogen lamps which are connected in series.
4. The four-dimensional electrophoresis apparatus of claim 2 wherein: the light sensitive elements (622) are formed using a multi-channel, multi-spectral sensor arrangement in a strip.
5. The four-dimensional electrophoresis apparatus of claim 1 wherein: the driving motor (63) is a stepping motor or a direct current servo motor.
6. The four-dimensional electrophoresis apparatus of claim 1 wherein: the device is characterized by further comprising a PC (7), wherein the PC (7) is connected with the single chip microcomputer (61) and can receive signals sent by the single chip microcomputer (61) to process.
7. The four-dimensional electrophoresis apparatus of claim 1 wherein: the strong magnetic field is distributed in the front half part of the electrophoresis carrier (5), and the permanent magnet occupies 1/3-4/5 of the length of the electrophoresis carrier (5) relative to the direction of the force of the electric field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222425051.1U CN218382526U (en) | 2022-09-14 | 2022-09-14 | Four-dimensional electrophoresis apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222425051.1U CN218382526U (en) | 2022-09-14 | 2022-09-14 | Four-dimensional electrophoresis apparatus |
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| Publication Number | Publication Date |
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| CN218382526U true CN218382526U (en) | 2023-01-24 |
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| CN202222425051.1U Active CN218382526U (en) | 2022-09-14 | 2022-09-14 | Four-dimensional electrophoresis apparatus |
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| CN (1) | CN218382526U (en) |
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