CN219099190U

CN219099190U - CAR-T cell incubator with shaking table

Info

Publication number: CN219099190U
Application number: CN202223583727.6U
Authority: CN
Inventors: 卢民
Original assignee: Jinan Hongfengye Biotechnology Co ltd
Current assignee: Jinan Hongfengye Biotechnology Co ltd
Priority date: 2022-12-31
Filing date: 2022-12-31
Publication date: 2023-05-30
Anticipated expiration: 2032-12-31

Abstract

The utility model relates to the technical field of cell culture equipment, in particular to a CAR-T cell culture device with a shaking table, which comprises a machine body shell, wherein a control panel is fixedly connected to the outer side of the machine body shell, a sterile box is arranged in the machine body shell, connecting blocks are movably connected to the inner wall of the sterile box, a rotating rod is rotationally connected between the connecting blocks, a connecting plate is fixedly connected to the outer side of the rotating rod, a culture dish body is fixedly connected to the outer side of the connecting plate, a guide rod is fixedly connected to the top of the culture dish body, a buffer spring is fixedly connected to the outer side of the guide rod, a telescopic pipe is movably connected to the top of the guide rod, a magnetic sucker is fixedly connected to the top of the telescopic pipe, and a motor is fixedly arranged on the inner wall of the sterile box.

Description

CAR-T cell incubator with shaking table

Technical Field

The utility model relates to a CAR-T cell incubator with a shaking table, and belongs to the technical field of cell culture equipment.

Background

T cells, also called T lymphocytes, are one type of human leukocytes, originate from peripheral blood, play an immune role in the human body, and have the role equivalent to 'fighters' in the human body, and can resist and eliminate 'enemies' such as infection, tumor, foreign matters and the like. In the laboratory, technicians activate T cells by genetic engineering techniques and equip with a positioning navigation device CAR to transform the general "fighter" T cells into "super fighter", i.e. CAR-T cells.

Traditional CAR-T cell culture ware adopts the stirring mode mostly, but the cell is comparatively fragile, adopts the stirring mode shearing force great, and is great to the cell damage, is unfavorable for the culture to the cell, and cell culture dish generally all installs in aseptic environment, after the culture, need clear up the inside of culture dish, but the connected mode of current culture dish leads to it more troublesome when taking out, influences the cleaning efficiency of culture dish.

Disclosure of Invention

The utility model aims to provide a CAR-T cell incubator with a shaking table, which has the advantages of simple structure, convenience in use, capability of shaking the culture dish to reduce damage to cells, convenience in taking out the culture dish, and capability of rapidly cleaning the culture dish so as to solve the problems in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the utility model provides a CAR-T cell incubator with shaking table, includes engine body shell, engine body shell's outside fixedly connected with control panel, the aseptic case has been seted up to engine body shell's inside, swing joint has the adapter piece on the inner wall of aseptic case, rotate between the adapter piece and be connected with the rotary rod, the outside fixedly connected with connecting plate of rotary rod, the outside fixedly connected with culture dish body of connecting plate, the top fixedly connected with guide arm of culture dish body, the outside fixedly connected with buffer spring of guide arm, the top swing joint of guide arm has flexible pipe, the top fixedly connected with magnetic chuck of flexible pipe, fixedly connected with motor on the inner wall of aseptic case, the output fixedly connected with pivot of motor, the outside fixedly connected with swivelling ring of pivot, the outside fixedly connected with dwang of swivelling ring, the outside fixedly connected with extrusion ball of dwang, fixedly connected with firm pole on the inner wall of aseptic case, the inside of adapter piece has seted up the joint hole, threaded hole has been seted up to the inside of firm pole, the inside rotation of adapter piece is connected with screw rod fixedly connected with screw rod.

Further, the connection blocks are symmetrically distributed on the inner wall of the sterile box, and the culture dish body is movably connected with the connecting plate inside the sterile box through a rotating rod.

Further, the top of buffer spring fixed connection is in the bottom of flexible pipe, the top fixed connection of magnetic chuck is on the interior roof of aseptic case.

Further, the motor is electrically connected with the control panel, the number of the rotating rods is the same as that of the extrusion balls, and the rotating rods and the extrusion balls are symmetrically distributed on the outer side of the rotating ring.

Further, the rotating rod and the extrusion ball are positioned right below the culture dish body, and the connecting block is movably connected to the outer side of the inner wall of the sterile box through the stabilizing rod.

Further, the bottom of the screw rod is rotationally connected in the threaded hole of the inner wall of the stabilizing rod, and the outer threads of the screw rod are meshed with the threads of the inner wall of the threaded hole.

The beneficial effects of the utility model are as follows:

according to the utility model, the machine body shell is arranged, the sterile box is arranged in the machine body shell, the connecting blocks are arranged in the sterile box, the rotary rods are rotatably connected between the connecting blocks, the culture dish body is movably connected in the sterile box through the matched use of the rotary rods and the connecting plates, the motor is started through the control panel, so that the motor drives the rotary shaft to rotate, the rotary ring is driven to rotate through the rotation of the rotary shaft, at the moment, the rotary rod and the extrusion ball outside the rotary ring intermittently extrude the culture dish body, and then the connecting rods at the top of the culture dish body are matched with the telescopic tubes, so that the culture dish body shakes back and forth in the sterile box, and the buffer springs are fixedly connected to the outer sides of the guide rods, therefore, the shaking amplitude of the culture dish body is not very large, the effects of effective mixing and no damage to cells are achieved, and when the culture dish body is required to be taken out for cleaning, the transmission cover drives the screw rod to rotate in the interior of the connecting blocks, and the bottom plate of the screw rod is rotatably connected in the threaded hole inside the rod, when the screw rod leaves the interior of the threaded hole, the sterile box can be removed from the sterile box, and the sterile culture dish body can be conveniently cleaned.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model, and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the description serve to explain the utility model.

FIG. 1 is a schematic diagram of the structure of a CAR-T cell incubator with shaker of the present utility model;

FIG. 2 is a schematic view showing the structure of the outer side of a culture dish body of a CAR-T cell culture apparatus with a shaking table according to the present utility model;

FIG. 3 is a schematic diagram showing the structure of the motor and the outside of the rotating shaft of the CAR-T cell incubator with a shaker according to the present utility model;

FIG. 4 is an enlarged view of the structure at A in FIG. 2 of a CAR-T cell incubator with shaker according to the present utility model;

reference numerals in the drawings: 1. a housing; 2. a control panel; 3. a sterile box; 4. a joint block; 5. a rotating rod; 6. a connecting plate; 7. a culture dish body; 8. a guide rod; 9. a buffer spring; 10. a telescopic tube; 11. a magnetic chuck; 12. a motor; 13. a rotating shaft; 14. a rotating ring; 15. a rotating lever; 16. extruding the ball; 17. a stabilizing rod; 18. a clamping hole; 19. a threaded hole; 20. a screw; 21. a transmission cover.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Embodiment 1 referring to fig. 1 to 4, the present utility model provides a technical solution:

the CAR-T cell incubator with the shaking table comprises an organism shell 1, a control panel 2 is fixedly connected to the outer side of the organism shell 1, an aseptic box 3 is arranged in the organism shell 1, a connecting block 4 is movably connected to the inner wall of the aseptic box 3, a rotating rod 5 is connected between the connecting blocks 4 in a rotating mode, a connecting plate 6 is fixedly connected to the outer side of the rotating rod 5, a culture dish body 7 is fixedly connected to the outer side of the connecting plate 6, a guide rod 8 is fixedly connected to the top of the culture dish body 7, a buffer spring 9 is fixedly connected to the outer side of the guide rod 8, a telescopic tube 10 is movably connected to the top of the guide rod 8, a magnetic sucker 11 is fixedly connected to the top of the telescopic tube 10, a motor 12 is fixedly connected to the inner wall of the aseptic box 3, a rotating shaft 13 is fixedly connected to the output end of the motor 12, a rotating ring 14 is fixedly connected to the outer side of the rotating ring 14, a rotating rod 15 is fixedly connected to the outer side of the rotating rod 15, and an extrusion ball 16 is fixedly connected to the outer side of the rotating rod 15.

Specifically, as shown in fig. 1, the connection blocks 4 are symmetrically distributed on the inner wall of the aseptic tank 3, the culture dish body 7 is movably connected with the connecting plate 6 inside the aseptic tank 3 through the rotary rod 5, the top of the buffer spring 9 is fixedly connected with the bottom of the telescopic tube 10, and the top of the magnetic chuck 11 is fixedly connected with the inner top wall of the aseptic tank 3.

Specifically, as shown in fig. 1-4, the motor 12 is electrically connected with the control panel 2, the number of the rotating rods 15 and the number of the extrusion balls 16 are the same, the rotating rods 15 and the extrusion balls 16 are symmetrically distributed on the outer side of the rotating ring 14, the rotating rods 15 and the extrusion balls 16 are located right below the culture dish body 7, the connecting block 4 is movably connected on the outer side of the inner wall of the sterile box 3 through the stabilizing rod 17, the bottom of the screw rod 20 is rotatably connected in a threaded hole 19 on the inner wall of the stabilizing rod 17, and the outer threads of the screw rod 20 are meshed with the threads on the inner wall of the threaded hole 19.

Embodiment 2 referring to fig. 1, 2 and 4, the difference between the present embodiment and embodiment 1 is that: fixedly connected with firm pole 17 on the inner wall of aseptic case 3, joint hole 18 has been seted up to the inside of linking block 4, and screw hole 19 has been seted up to the inside of firm pole 17, and the inside rotation of linking block 4 is connected with screw rod 20, and screw rod 20's top fixedly connected with transmission cover 21.

The working principle of the utility model is as follows: through having set up engine body shell 1, the aseptic case 3 has been seted up in the inside of engine body shell 1, through the internally mounted of aseptic case 3 has joint piece 4, owing to rotate between joint piece 4 and be connected with rotary rod 5, through the cooperation use of rotary rod 5 and connecting plate 6, make culture dish body 7 swing joint in the inside of aseptic case 3, make motor 12 start through control panel 2, thereby make motor 12 drive pivot 13 rotate, the rotatory circle 14 that drives through pivot 13 rotates, the dwang 15 in the rotatory circle 14 outside at this moment can intermittent type produce the extrusion to culture dish body 7 with the extrusion ball 16, rethread culture dish body 7 top guide arm 8 and flexible pipe 10's cooperation is used, thereby make culture dish body 7 make a round trip to rock in the inside of aseptic case 3, and in the outside fixedly connected with buffer spring 9 of guide arm 8, therefore culture dish body 7 rocks the range can not be very big, the effect that has reached effective mixing and can not destroy the cell, when need take out culture dish body 7 and wash, through rotating the transmission cover 21 drives screw rod 20 and rotates in the inside of joint piece 4 at this moment, the inside of joint piece 4 rotates 15 and extrusion ball 16 can intermittent to produce the extrusion, the cooperation use of take out at the inside of aseptic case 3 at this moment, the time, the inside of take out screw rod 19 is followed the inside of the aseptic case 19 is followed in the screw rod 19, the inside has reached the aseptic case 3, and has been removed from the inside of the aseptic case 3, and has been made the effect of the screw rod 19.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. CAR-T cell culture ware with shaking table, including organism shell (1), its characterized in that: the utility model discloses a sterile box (3) is offered to the outside fixedly connected with control panel (2) of organism shell (1), aseptic box (3) is offered to the inside of organism shell (1), swing joint has link up piece (4) on the inner wall of aseptic box (3), rotate between link up piece (4) and be connected with rotary rod (5), the outside fixedly connected with connecting plate (6) of rotary rod (5), the outside fixedly connected with culture dish body (7) of connecting plate (6), the top fixedly connected with guide arm (8) of culture dish body (7), the outside fixedly connected with buffer spring (9) of guide arm (8), the top swing joint of guide arm (8) flexible pipe (10), the top fixedly connected with magnetic chuck (11) of flexible pipe (10), fixedly mounted motor (12) on the inner wall of aseptic box (3), the output fixedly connected with pivot (13) of motor (12), the outside fixedly connected with rotation collar (14), the outside fixedly connected with rotation lever (15) of rotation collar (15), the inside fixedly connected with extrusion ball (16) of aseptic box (3) is connected with firm connection hole (18), threaded holes (19) are formed in the stabilizing rod (17), a screw (20) is rotatably connected to the inside of the connecting block (4), and a transmission cover (21) is fixedly connected to the top of the screw (20).

2. A CAR-T cell culture apparatus with a shaker as claimed in claim 1, wherein: the connecting blocks (4) are symmetrically distributed on the inner wall of the aseptic tank (3), and the culture dish body (7) is movably connected with the connecting plate (6) through the rotating rod (5) in the aseptic tank (3).

3. A CAR-T cell culture apparatus with a shaker as claimed in claim 1, wherein: the top of the buffer spring (9) is fixedly connected to the bottom of the telescopic tube (10), and the top of the magnetic sucker (11) is fixedly connected to the inner top wall of the sterile box (3).

4. A CAR-T cell culture apparatus with a shaker as claimed in claim 1, wherein: the motor (12) is electrically connected with the control panel (2), the number of the rotating rods (15) is the same as that of the extrusion balls (16), and the rotating rods (15) and the extrusion balls (16) are symmetrically distributed on the outer side of the rotating ring (14).

5. A CAR-T cell culture apparatus with a shaker as claimed in claim 1, wherein: the rotating rod (15) and the extrusion ball (16) are positioned right below the culture dish body (7), and the connecting block (4) is movably connected to the outer side of the inner wall of the sterile box (3) through the stabilizing rod (17).

6. A CAR-T cell culture apparatus with a shaker as claimed in claim 1, wherein: the bottom of the screw rod (20) is rotatably connected in a threaded hole (19) in the inner wall of the stabilizing rod (17), and the outer threads of the screw rod (20) are meshed with the threads in the inner wall of the threaded hole (19).