CN219079491U - Device for extracting single cell suspension from large-capacity tissue fragments - Google Patents

Abstract

The utility model provides a device for extracting single-cell suspension from large-capacity tissue fragments, which aims to solve the problems of complex operation and easy pollution in the process of extracting single-cell suspension from the tissue fragments.

Description

Device for extracting single cell suspension from large-capacity tissue fragments

Technical Field

The utility model relates to the technical field of cell culture devices, in particular to a device for extracting single cell suspension from large-capacity tissue fragments.

Background

Cell culture refers to a method of simulating in vitro an in vivo environment to grow, reproduce and maintain the major structure and function of cells. Cell culture, also known as cell cloning technology, is an essential step in both biomedical research and development and in the production of cell products. The cell culture technique can obtain a target cell line by a cell which is subjected to a large number of culture to become simple single cell clone or directed differentiation into a target cell, and can also study signal transduction of the cell, anabolism of the cell, growth and proliferation of the cell, and the like.

In a typical animal cell separation culture experiment, cells at other positions except blood cells are separated and cultured, firstly, tissue blocks at the positions are collected in the first step, and then single cells are formed by mechanical shearing, enzymolysis or combination of the two means. Then screening the cells, filtering out residual tissue blocks and cell clusters to obtain single-cell suspension, removing erythrocyte enriched nucleated cells through the steps of density gradient centrifugation, erythrocyte lysis and the like, and then carrying out subsequent cell analysis or pure culture of target cells by using a specific culture medium.

In the prior art, animal tissues are subjected to enzymolysis digestion or mechanical disruption, and then the treated mixture is screened by a cell sieve and is washed by a large amount of buffer solution or culture medium to collect single cells as much as possible, so that the defects are that: firstly, the operation process is open operation, so that the risk of microbial pollution is increased; secondly, the sizes of tissue fragments are different, the screen is easy to block, the screen is required to be replaced for a plurality of times or coarse, medium and fine screens are adopted for filtering in sequence, the single cell suspension collecting process can be completed, the time consumption is long, and the pollution probability is further increased.

Disclosure of Invention

In order to solve the problems of complex operation and easy pollution in the process of extracting single cell suspension from tissue fragments, the utility model provides a device for extracting single cell suspension from large-capacity tissue fragments, which adopts the following technical scheme:

the utility model provides a single cell suspension device is drawed to large capacity tissue fragment, its characterized in that includes the ladle body, sets up in the inside cell screen cloth of ladle body and mixing ware, the ladle body comprises the lower ladle body of last ladle body detachable connection in last ladle body below, and upper ladle body and lower ladle body lateral wall all are provided with the screen card groove, and screen card inslot joint has the cell screen cloth, and upper ladle body upper end is connected with the block, be provided with first silica gel contact pin mouth on the block, upper ladle body inlet department fixedly connected with and the second silica gel contact pin mouth of first silica gel contact pin mouth position correspondence distribution, the inside washing liquid pipe that is provided with of upper ladle body, mixing ware, washing liquid pipe one end links to each other with second silica gel contact pin mouth, and washing liquid pipe liquid outlet is located mixing ware top, still is provided with mixing ware draw-in groove on the upper ladle body lateral wall, mixing ware rotates through mixing ware draw-in groove to be connected in the inside and be located the top of upper ladle body cell screen cloth, lower ladle body lower extreme is connected with the drain pipe, is provided with drain pipe on the drain pipe.

Preferably, the washing liquid pipe comprises single pipe section and annular section, the single pipe section is fixed with last ladle body inner wall, and one end links to each other with the second silica gel contact pin mouth, and the other end links to each other with the annular section, the annular section distributes along the horizontal direction and is provided with a plurality of branch liquid holes along the circumferencial direction.

Preferably, the blender comprises blender frame, the inside blending strip of fixed connection in blender frame and the blender handle of fixed connection in the blender frame outside, the blender frame rotates along the blender draw-in groove and the joint links to each other, the blender handle stretches out the upper ladle body outside.

Preferably, the side walls of the upper barrel body and the lower barrel body are provided with transparent windows.

Preferably, the cap is further provided with an air filter membrane.

The utility model has the beneficial effects that:

1. the washing liquid guide pipe, the mixer and the plurality of groups of cell screens are arranged in the whole device from top to bottom, the mixing and filtering operations can be performed simultaneously, single cells in the tissue fragments are pressed to the greatest extent in a short time, the activity and the density of the cells are effectively improved, the time is saved, the tightness of the whole device is good, and the pollution risk is effectively reduced in the process of cleaning the tissue fragments and collecting single cell suspension;

2. the inside of the barrel body is provided with a plurality of cell screen card slots from top to bottom, and screens with different apertures can be selected for combined use according to specific conditions of tissue fragments in the actual use process, so that the problem of blocking of the cell screens is effectively solved, and the filtering efficiency is improved;

3. the washing liquid conduit consists of a straight pipe section and an annular section, and washing liquid can be uniformly fed onto the tissue fragments through multi-component liquid holes arranged on the annular section, so that the holes are prevented from being caused by overlarge local impact;

4. through setting up air filter on the block, guarantee that the device internal and external pressure differential is unanimous, ladle body lateral wall is through setting up transparent window and is convenient to observe the interior washing liquid volume of device.

Drawings

FIG. 1 is a schematic view of the structure of the present utility model

FIG. 2 is a schematic diagram of an explosion structure according to the present utility model

FIG. 3 is a schematic view of the partial structure of FIG. 1

FIG. 4 is a top view of the cap

FIG. 5 is a top view of a circular section of a wash conduit

FIG. 6 is a top view of a cell screen

FIG. 7 is a top view of the mixer

The device comprises a 1-cap, a 11-first silica gel contact pin opening, a 12-air filter membrane, a 2-upper barrel body, a 21-first silica gel sealing ring, a 22-mixer clamping groove, a 23-first-stage screen card groove, a 24-second silica gel sealing ring, a 25-second silica gel contact pin opening, a 26-washing liquid guide pipe, a 261-single pipe section, a 262-annular section, a 2621-liquid separation hole, a 263-washing liquid guide pipe fixing frame, a 27-transparent window, a 3-lower barrel body, a 31-second-stage screen card groove, a 32-third-stage screen card groove, a 33-liquid outlet, a 34-liquid outlet pipe, a 35-liquid outlet switch, a 36-supporting leg, a 4-cell screen, a 41-screen handle, a 42-cell screen main body, a 43-cell screen sealing frame, a 5-mixer, a 51-mixer handle, a 52-mixing bar and a 53-mixer sealing frame.

Detailed Description

Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

In the description of the utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the view direction or positional relationships, merely to facilitate describing the utility model, and do not indicate or imply that the devices or elements being referred to must have a particular orientation, be configured and operated in a particular orientation, and thus should not be construed as limiting the utility model.

The device for extracting single cell suspension from large-capacity tissue fragments as shown in fig. 1-7 comprises an upper barrel body 2 and a lower barrel body 3, wherein the upper barrel body 2 is detachably connected above the lower barrel body 3, a mixer 5 is arranged in the upper barrel body 2, and cell screens 4 are arranged in the upper barrel body 2 and the lower barrel body 3.

Specifically, as shown in fig. 2 and 3, the upper end of the upper barrel body 2 is provided with a liquid inlet, a cap 1 is connected above the liquid inlet, a first silica gel sealing ring 21 is arranged between the cap 1 and the liquid inlet at the upper end of the upper barrel body 2, the lower end of the upper barrel body 2 is in threaded connection with the upper end of the lower barrel body 3, a second silica gel sealing ring 24 is arranged at the joint of the upper end of the upper barrel body 2 and the upper end, a first silica gel contact pin interface 11 and a second silica gel contact pin interface 25 which are distributed vertically and correspondingly are fixedly connected to the liquid inlet at the upper end of the cap 1 and the upper barrel body 2 respectively are sequentially arranged in the upper barrel body 2 from top to bottom, a washing liquid conduit 26, a mixer 5 and a cell screen 4 are sequentially arranged on the side wall of the upper barrel body 2, a semicircular mixer clamping groove 22 and a first-stage screen card groove 23 positioned below the mixer clamping groove 22 are arranged on the side wall of the upper barrel body 2, the mixer 5 is clamped inside the upper barrel body 2, the mixer 5 can rotate along the length direction of the mixer clamping groove 22, and the cell 4 is clamped inside the upper barrel body 2 through the first-stage screen card groove 23.

The cell screen 4 is shown in fig. 6, by cell screen handle 41, cell screen main part 42 and cell screen seal frame 43 are constituteed, cell screen main part 42 is fixed inside cell screen seal frame 43, cell screen handle 41 fixed connection is in cell screen seal frame 43 outside, cell screen seal frame 43 links to each other and seals with three group of cell screen card groove joint on last ladle body 2 and the lower ladle body 3 lateral wall, and cell screen main part 42 wholly is located inside last ladle body 2 and lower ladle body 3, in this embodiment, through setting up three group of screen card grooves, can select cell screen 4 combination use of different apertures according to the tissue fragment specific condition, in order to solve the cell screen jam problem, improve filtration efficiency.

The washing liquid pipe 26 is shown in fig. 3 and 5, and comprises single pipe 261 and annular section 262, and single pipe 261 one end links to each other with second silica gel contact pin interface 25, and the other end links to each other with annular section 262, and single pipe 261 is fixed through the multiunit washing liquid pipe mount 263 of fixing on last ladle body 2 inner wall, and annular section 262 wholly is the level and with last ladle body 2 coaxial distribution, and annular section 262 evenly is provided with a plurality of branch liquid holes 2621 along the circumferencial direction, can make washing liquid uniform flow add on the tissue fragment through a plurality of branch liquid holes 2621 on the annular section 262, prevents that local impact is too big, causes the hole.

The mixer 5 is shown in fig. 2 and 7, and is composed of a mixer handle 51, mixing strips 52 and a mixer sealing frame 53, wherein a plurality of groups of mixing strips 52 are distributed and fixed inside the mixer sealing frame 53 in a spoke shape, the mixer handle 51 is fixedly connected to the outer side of the mixer sealing frame 53, the mixer sealing frame 53 extends into the upper barrel body 2 through a mixer clamping groove 22, and the mixer sealing frame 53 can rotate along the mixer clamping groove 22.

The lower ladle body 3 is as shown in fig. 2, and below fixedly connected with landing leg 36, and the lower extreme is provided with liquid outlet 33, and liquid outlet 33 is connected with drain pipe 34, is provided with out liquid switch 35 on the drain pipe 34, and lower ladle body 3 lateral wall top-down is provided with semi-circular structure's second grade screen card slot 31 and tertiary screen card slot 32, and cell screen 4 passes through second grade screen card slot 31 and tertiary screen card slot 32 joint in lower ladle body 2 inside.

In addition, transparent windows 27 are arranged on the side walls of the upper barrel body 2 and the lower barrel body 3 and used for observing the liquid washing amounts in the upper barrel body 2 and the lower barrel body 3, the cap 1 is provided with an air filter membrane 12 in the middle part as shown in fig. 4, and the consistency of the pressure difference between the inside and the outside of the barrel bodies is ensured.

The single cell suspension is extracted from the tissue fragments by using the device as follows: firstly, sterilizing the whole device at high temperature, after the biological safety cabinet is used for assembling the whole device, one end of a liquid outlet pipe 34 is inserted into a sterile cell collecting bag, a liquid outlet switch 35 is opened, and a cell washing liquid bag is hung at a high position for standby; opening the cap 1, adding the processed tissue fragments into the barrel body, screwing the cap 1, simultaneously paying attention to aligning the first silica gel pin port 11 with the second silica gel pin port 25, then rotating the mixer 5 to uniformly spread tissue blocks on the first-stage cell screen 4, sequentially penetrating the needle heads of the washing liquid bags through the first silica gel pin port 11 and the second silica gel pin port 25, then inserting the washing liquid bags into the washing liquid guide pipe 26, enabling the washing liquid to flow out through the liquid separation holes 2621 and uniformly spray on the tissue fragments, washing single-cell suspension through the multi-stage cell screen 4, enabling the single-cell suspension to flow into the cell collection bag after sequentially passing through the liquid outlet 33, the liquid outlet pipe 34 and the liquid outlet switch 35, and uniformly mixing the tissue blocks by rotating the mixer 5 in the operation process so as to improve the filtering effect.

The foregoing is only a preferred embodiment of the utility model, it being noted that: it will be apparent to those skilled in the art that modifications may be made without departing from the principles of the utility model, and such modifications are intended to be within the scope of the utility model.

Claims (5)

1. The utility model provides a single cell suspension device is drawed to large capacity tissue fragment, its characterized in that includes the ladle body, sets up in the inside cell screen cloth of ladle body and mixing ware, the ladle body comprises the lower ladle body of last ladle body detachable connection in last ladle body below, and upper ladle body and lower ladle body lateral wall all are provided with the screen card groove, and screen card inslot joint has the cell screen cloth, and upper ladle body upper end is connected with the block, be provided with first silica gel contact pin mouth on the block, upper ladle body inlet department fixedly connected with and the second silica gel contact pin mouth of first silica gel contact pin mouth position correspondence distribution, the inside washing liquid pipe that is provided with of upper ladle body, mixing ware, washing liquid pipe one end links to each other with second silica gel contact pin mouth, and washing liquid pipe liquid outlet is located mixing ware top, still is provided with mixing ware draw-in groove on the upper ladle body lateral wall, mixing ware rotates through mixing ware draw-in groove to be connected in the inside and be located the top of upper ladle body cell screen cloth, lower ladle body lower extreme is connected with the drain pipe, is provided with drain pipe on the drain pipe.

2. The device for extracting single-cell suspension from large-capacity tissue fragments according to claim 1, wherein the washing liquid conduit is composed of a single pipe section and an annular section, the single pipe section is fixed with the inner wall of the upper barrel body, one end of the single pipe section is connected with the second silica gel contact pin port, the other end of the single pipe section is connected with the annular section, and the annular section is distributed along the horizontal direction and provided with a plurality of liquid distributing holes along the circumferential direction.

3. The device for extracting single-cell suspension from high-capacity tissue fragments according to claim 1, wherein the homogenizer comprises a homogenizer frame, a homogenizing strip fixedly connected to the interior of the homogenizer frame, and a homogenizer handle fixedly connected to the outer side of the homogenizer frame, the homogenizer frame rotates along a homogenizer clamping groove and is connected in a clamping manner, and the homogenizer handle extends out of the upper barrel body.

4. The device for extracting single cell suspension from large volume tissue fragments according to claim 1, wherein transparent windows are provided on the side walls of the upper and lower barrels.

5. The device for extracting single cell suspension from large volume tissue fragments according to claim 1, wherein an air filter is further provided on said cap.

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