CN215162605U

CN215162605U - Umbilical cord mesenchymal stem cell extraction and separation device

Info

Publication number: CN215162605U
Application number: CN202022905033.4U
Authority: CN
Inventors: 万笑宇
Original assignee: Soyounglife Technology Co ltd
Current assignee: Soyounglife Technology Co ltd
Priority date: 2020-12-04
Filing date: 2020-12-04
Publication date: 2021-12-14
Anticipated expiration: 2030-12-04

Abstract

The utility model provides an umbilical cord mesenchymal stem cell extraction and separation device, which comprises an upper shell with a bottom surface, a lower shell and an upper cover, wherein the bottom surface of the upper shell is placed on the lower shell, and the upper cover is covered on the upper shell; the bottom end of the lower shell is provided with an opening and closing door, the upper part of the inner wall of the lower shell is fixedly provided with a symmetrical hanging table, a cell collecting hopper is detachably placed on the hanging table, and the lower part of the collecting hopper is spirally and spirally connected with a cell collecting bottle; the bottom surface of the upper shell is V-shaped and is parallel to the collecting surface of the collecting hopper, pointed protrusions and leak holes are arranged on the surface of the V-shaped surface at intervals, fixing holes are formed in the side wall of the upper shell, the cross rod penetrates through the holes, a downward elbow is arranged at one end of the cross rod in the upper shell, a V-shaped pointed hammer is arranged at the lower end of the elbow, the pointed protrusions are arranged on the surface of the pointed hammer, and the downward movement of the pointed hammer can be meshed with the pointed protrusions on the bottom surface of the upper shell in a staggered mode. The utility model discloses simple structure, convenient operation, furthest's assurance stem cell separation draws the in-process pollution-free.

Description

Umbilical cord mesenchymal stem cell extraction and separation device

Technical Field

The utility model relates to a stem cell draws technical field, in particular to umbilical cord mesenchymal stem cell draws separator.

Background

Umbilical cord Mesenchymal Stem Cells (MSCs) are a multifunctional Stem cell present in umbilical cord tissue of newborn, have high differentiation potential, and can be differentiated in multiple directions. It has wide clinical application prospect in the aspects of tissue engineering such as bones, cartilages, muscles, tendons, ligaments, nerves, livers, endothelia, cardiac muscles and the like.

Mesenchymal stem cells have been derived mainly from bone marrow and are a type of stem cells having high self-renewal and multipotential differentiation potential in bone marrow except Hematopoietic Stem Cells (HSCs). Under different induction conditions, the cells can be differentiated into a plurality of tissue cells except hematopoietic cells, and have the effects of hematopoietic support, immunoregulation, tissue repair and the like. However, with aging, the mesenchymal stem cells gradually decrease, the proliferation and differentiation capacity is greatly reduced, the donor is damaged by the material selection, and the application of the mesenchymal stem cells is limited. MSCs are separated from human umbilical cords in 2000 for the first time, the cell content and the proliferation capacity of the MSCs are superior to those of bone marrow MSCs, the immunogenicity of the MSCs is lower than that of the MSCs, and the MSCs have the advantages of convenient material taking, no ethical dispute and the like. And thus are receiving increasing attention from researchers.

The umbilical cord is the connective structure between the fetus and the placenta. It is in the shape of rope, has smooth and transparent surface, contains connective tissue, an umbilical vein and a pair of umbilical arteries. Cord blood stem cells can be isolated from blood contained in the umbilical cord of a newborn, and cord mesenchymal stem cells can be isolated from connective tissue.

In the preparation process of the umbilical cord mesenchymal stem cells, the whole fresh healthy umbilical cord is firstly separated into small sections with the length of about 1 cm-3 cm, and then the Huatong glue is stripped from the small sections. The umbilical cord is usually cut along the umbilical vein, after the umbilical cord is unfolded, the umbilical vein and two umbilical arteries are scraped off by a scalpel, the outermost membrane of the umbilical cord is removed by the scalpel, and the remaining white jelly-like substance is the huatong glue. Then the Huatong glue is sufficiently sheared into 1mm³After the size and digestion, the resulting tissue was cultured in an alpha-MEM medium containing penicillin and streptomycin at 37 ℃ in a 5% CO2 incubator. After the umbilical cord tissue is cultured for 5-7 days, partial cells climb out from the periphery of the tissue block and are in a fine spindle shape. After one week, the cells began to proliferate rapidly, forming colonies of cells of varying sizes. After the cells were confluent, 0 was used.And (5) digesting and passaging by 25% trypsin to obtain the umbilical cord mesenchymal stem cells.

The prior art cannot avoid biological pollution in the extraction process, so high-concentration streptomycin needs to be used, but the use of the high-concentration streptomycin can reduce the in-vivo homing and adhesion performance of the umbilical cord MSC. Is not beneficial to later-period scientific research and use.

SUMMERY OF THE UTILITY MODEL

In view of this, in order to overcome the not enough of prior art, the utility model provides an umbilical cord mesenchymal stem cells draws separator that can guarantee aseptic technique process.

The utility model provides an extraction and separation device for umbilical cord mesenchymal stem cells, which comprises an upper shell with a bottom surface, a lower shell and an upper cover, wherein the bottom surface of the upper shell is placed on the lower shell, and the upper cover is covered on the upper shell; the bottom end of the lower shell is provided with an opening and closing door, the upper part of the inner wall of the lower shell is fixedly provided with a symmetrical hanging table, a cell collecting hopper is detachably placed on the hanging table, and the lower part of the collecting hopper is spirally and spirally connected with a cell collecting bottle; the bottom surface of the upper shell is V-shaped and is parallel to the collecting surface of the collecting hopper, pointed protrusions and leak holes are arranged on the surface of the V-shaped surface at intervals, fixing holes are formed in the side wall of the upper shell, the cross rod penetrates through the holes, a downward elbow is arranged at one end of the cross rod in the upper shell, a V-shaped pointed hammer is arranged at the lower end of the elbow, the pointed protrusions are arranged on the surface of the pointed hammer, and the downward movement of the pointed hammer can be meshed with the pointed protrusions on the bottom surface of the upper shell in a staggered mode.

Further, the upper cover is provided with a handle, and a feeding hole is formed in the side of the upper cover.

Furthermore, the fixing holes are fixedly sealed with the cross rod through elastic sealing rubber rings.

Further, the cross rod is divided into an upper shell inner part and an upper shell outer part, and the inner part and the outer part of the cross rod are connected into a whole in an elastic sealing rubber ring in a spiral mode.

Further, a manual handle is arranged at the end part of the outer part of the cross rod.

Furthermore, the aperture of the leak hole is 1-5 mm.

Furthermore, the lower part of the collecting hopper is a hollow cylinder, the inner vertical cylinder of the collecting hopper is provided with a filtering membrane, and the outer wall of the collecting hopper is provided with threads which are spirally and spirally connected with the cell collecting bottle.

Furthermore, the filtering membranes are divided into two layers and respectively clamped between the two annular flanges on the inner wall of the hollow cylinder.

Furthermore, the aperture of the upper filter membrane of the two filter membranes is 1-3 mm.

Furthermore, the aperture of the lower filter membrane of the two layers of filter membranes is 1 mm.

When in use, the huatong glue and the digestive enzyme liquid obtained by stripping the fresh healthy umbilical cord slide into the bottom of the upper shell from the feed hole along the inner wall of the upper shell. The staff pulls the manual handle up and down, and the horizontal pole uses the fixed orifices to do lever motion as the fulcrum. When the manual handle moves upwards, the pointed hammer extrudes downwards with the bottom surface of the upper shell, the pointed protrusions on the pointed hammer are meshed with the pointed protrusions on the bottom surface of the upper shell in a staggered manner, the huatong glue is crushed into blocks, small tissue blocks enter the collecting hopper from the leakage holes, the tissue blocks and digestive enzymes are further digested in the collecting hopper, and then the small cell blocks enter the cell collecting bottle after being filtered by the filter membrane twice.

When the cells collected by the cell collecting bottle are close to the bottle mouth, the staff unscrews the cell collecting bottle from the collecting hopper through the opening and closing door and takes out the cell collecting bottle. The freshly sterilized cell collection vial was re-spun. The removed cell collection bottle can be directly used for cell inoculation culture.

When the collecting bucket is used, the upper cover is opened, the cross rod is unscrewed into two parts from the elastic sealing rubber ring, the part with the sharp hammer is taken out, and the collecting bucket is detached from the hanging table. Cleaning the upper cover, the sharp hammer, the upper shell and the collecting hopper. After cleaning, inserting one end with a sharp hammer into the fixing position of the elastic sealing rubber ring, sterilizing the elastic sealing rubber ring and the upper shell together, and respectively sterilizing the upper cover and the collecting hopper. And then assembled into a use state.

The beneficial effects of the utility model reside in that:

1. the utility model discloses simple structure, convenient operation. The tissue is broken thoroughly and fully in the whole process, and the time consumption is low.

2. Can realize the aseptic operation process and furthest ensure no pollution in the process of separating and extracting the stem cells. Thereby reducing the use concentration of the streptomycin and being beneficial to the scientific research and the use of stem cells in the later period.

3. The utility model discloses can disassemble the washing between pointed hammer and the last casing, integrative sterilization, it is all very convenient to wash and use to disinfect.

Drawings

FIG. 1: the utility model discloses a schematic structural diagram of an umbilical cord mesenchymal stem cell extraction and separation device;

FIG. 2: the structure of the hollow cylinder at the bottom of the collecting hopper is shown schematically;

wherein:

1-upper cover, 2-handle, 3-feed inlet, 4-upper shell, 5-manual handle, 6-elbow, 7-pointed hammer, 8-pointed projection on the hammer, 9-upper shell bottom surface, 10-leak hole, 11-pointed projection on the bottom surface, 12-collection bucket, 13-cell collection bottle, 14-opening and closing door, 15-hanging platform, 16-lower shell, 17-collection bucket outer edge, 18-filtration membrane clamping edge, 19-external thread, 20-fixed hole and 21-cross bar.

The specific implementation mode is as follows:

the following examples are illustrative of the present invention and the present invention is not limited to the following examples.

Example 1

As shown in fig. 1, the device for extracting and separating umbilical cord mesenchymal stem cells of the present invention comprises an upper casing 4 having a bottom surface 9, a lower casing 16 and an upper cover 1, wherein the bottom surface 9 of the upper casing is placed on the lower casing 16, and the upper cover 1 covers the upper casing 4; the bottom end of the lower shell 16 is provided with an opening and closing door 14, the upper part in the lower shell 16 is fixedly provided with a symmetrical hanging platform 15, a cell collecting hopper 12 is detachably arranged on the hanging platform 15, and the lower part of the collecting hopper 12 is spirally and spirally connected with a cell collecting bottle 13; the bottom of the upper shell 4 is V-shaped and is parallel to a collecting surface of the collecting hopper, the V-shaped surface is provided with a pointed protrusion 11 which is arranged in the upper shell at intervals, a fixing hole 20 is arranged on the side wall of the upper shell 4 of the leak hole 10, a cross rod 21 penetrates through the hole, one end of the cross rod 21 in the upper shell 4 is provided with a downward elbow 6, the lower end of the elbow 6 is provided with a V-shaped pointed hammer 7, the surface of the pointed hammer 7 is provided with a pointed protrusion 8, and the downward movement of the pointed hammer 7 can be meshed with the pointed protrusion 11 on the bottom surface of the upper shell 4 in a staggered manner.

The upper cover 1 is provided with a handle 2, and a feeding hole 3 is arranged beside the upper cover.

The fixing hole 20 is fixedly sealed with the cross rod 21 through an elastic sealing rubber ring.

The cross bar 21 is divided into an upper shell inner part and an upper shell outer part, and the inner part and the outer part of the cross bar 21 are spirally connected into a whole at the elastic sealing rubber ring.

The end of the outer part of the cross bar 21 is provided with a manual handle 5.

The aperture of the leak hole is 5 mm.

The lower part of the collecting hopper 12 is a hollow cylinder, the inner vertical cylinder is provided with a filtering membrane, the outer wall of the collecting hopper is provided with threads 19, and the collecting hopper is screwed with the cell collecting bottle 13.

The filtering membranes are divided into two layers and are respectively clamped between the two annular flanges 18 on the inner wall of the hollow cylinder.

The aperture of the upper filter membrane of the two filter membranes is 3 mm.

And the aperture of the lower filter membrane of the two layers of filter membranes is 1 mm.

When in use, the huatong glue and the digestive enzyme liquid obtained by stripping the fresh healthy umbilical cord slide into the bottom of the upper shell from the feed hole 3 along the inner wall of the upper shell 4. The staff pulls the hand handle 5 up and down, the cross bar 21 makes lever motion with the fixed hole 20 as the fulcrum, when the hand handle 5 moves upwards, the pointed hammer 7 extrudes downwards with the bottom surface of the upper shell 4, the pointed protrusion 7 on the pointed hammer 7 is engaged with the pointed protrusion 11 on the bottom surface of the upper shell 4 in a staggered way, Fahrenheit glue is broken into blocks, small tissue blocks enter the collecting hopper 12 from the leakage hole 10, the tissue blocks and digestive enzyme in the collecting hopper 12 are further digested together, and then the smaller cell blocks enter the cell collecting bottle 13 after twice filtration by the filter membrane.

When the cells collected in the cell collection bottle 13 approach the opening of the bottle, the worker unscrews the cell collection bottle 13 from the collection funnel 12 through the opening/closing door 14 and takes out the cell collection bottle. The freshly sterilized cell collection vial was re-spun and used. The removed cell collection bottle 13 can be directly used for seeding culture of stem cells.

After use, the upper cover 1 is opened, the cross rod 21 is screwed off from the elastic sealing rubber ring, the part with the sharp hammer 7 is taken out, and the collecting hopper 12 is detached from the hanging platform 15. Cleaning the upper cover 1, the pointed hammer 7, the upper shell 4 and the collecting hopper 12. After cleaning, inserting one end with a sharp hammer into the fixing position of the elastic sealing rubber ring, sterilizing the elastic sealing rubber ring and the upper shell together, and respectively sterilizing the upper cover and the collecting hopper. And then assembled into a use state.

The bottom surfaces of the pointed hammer and the upper shell are made of metal materials, and the other parts of the pointed hammer and the bottom surface of the upper shell are made of metal or polytetrafluoroethylene materials.

As shown in fig. 2, the filter membrane clamping rim 18 may be formed by spirally winding the upper and lower tubular bodies to clamp the filter membrane therebetween.

Although the embodiments have been described in detail with reference to the above embodiments, those skilled in the art can still modify or substitute the embodiments of the present invention without departing from the spirit and scope of the present invention, which is defined by the appended claims.

Claims

1. The utility model provides an umbilical cord mesenchymal stem cell draws separator which characterized in that: the device comprises an upper shell with a bottom surface, a lower shell and an upper cover, wherein the bottom surface of the upper shell is placed on the lower shell, and the upper cover covers the upper shell; the bottom end of the lower shell is provided with an opening and closing door, the upper part of the inner wall of the lower shell is fixedly provided with a symmetrical hanging table, a cell collecting hopper is detachably placed on the hanging table, and the lower part of the collecting hopper is spirally and spirally connected with a cell collecting bottle; the bottom surface of the upper shell is V-shaped and is parallel to the collecting surface of the collecting hopper, pointed protrusions and leak holes are arranged on the surface of the V-shaped surface at intervals, fixing holes are formed in the side wall of the upper shell, the cross rod penetrates through the holes, a downward elbow is arranged at one end of the cross rod in the upper shell, a V-shaped pointed hammer is arranged at the lower end of the elbow, the pointed protrusions are arranged on the surface of the pointed hammer, and the downward movement of the pointed hammer can be meshed with the pointed protrusions on the bottom surface of the upper shell in a staggered mode.

2. The stem cell extraction and separation device according to claim 1, wherein: the upper cover is provided with a handle, and the side of the upper cover is provided with a feeding hole.

3. The stem cell extraction and separation device according to claim 1, wherein: the fixing holes are fixedly sealed with the cross rod through elastic sealing rubber rings.

4. The stem cell extraction and separation device according to claim 3, wherein: the cross rod is divided into an upper shell inner part and an upper shell outer part, and the inner part and the outer part of the cross rod are connected into a whole in an elastic sealing rubber ring in a spiral mode.

5. The stem cell extraction and separation device according to claim 4, wherein: and a manual handle is arranged at the end part of the outer part of the cross rod.

6. The stem cell extraction and separation device according to claim 1, wherein: the aperture of the leak hole is 1-5 mm.

7. The stem cell extraction and separation device according to claim 1, wherein: the lower part of the collecting hopper is a hollow cylinder, the inner vertical cylinder of the collecting hopper is provided with a filtering membrane, the outer wall of the collecting hopper is provided with threads, and the collecting hopper is spirally connected with the cell collecting bottle.

8. The stem cell extraction and separation device according to claim 7, wherein: the filtering membranes are divided into two layers and are respectively clamped between the two annular flanges on the inner wall of the hollow cylinder.

9. The stem cell extraction and separation device according to claim 8, wherein: the aperture of the upper filter membrane of the two filter membranes is 1-3 mm.

10. The stem cell extraction and separation device according to claim 8, wherein: and the aperture of the lower filter membrane of the two layers of filter membranes is 1 mm.