CN219010270U - Solid-liquid separation system of fermentation broth - Google Patents
Solid-liquid separation system of fermentation broth Download PDFInfo
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- CN219010270U CN219010270U CN202222893628.1U CN202222893628U CN219010270U CN 219010270 U CN219010270 U CN 219010270U CN 202222893628 U CN202222893628 U CN 202222893628U CN 219010270 U CN219010270 U CN 219010270U
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Abstract
The utility model relates to a solid-liquid separation system of fermentation liquor, which comprises a fermentation tank, ceramic membrane equipment and a permeate storage tank; the solid-liquid separation system is connected into a concentrated solution loop pipeline of the ceramic membrane equipment through the modification fermentation tank, and a circulating tank, a clean water storage tank and a cooling device which are configured by the ceramic membrane equipment are omitted. Compared with the prior ceramic membrane solid-liquid separation process, the configuration of a circulating tank is reduced, and the yield is improved by 14-20%.
Description
Technical Field
The utility model relates to a solid-liquid separation system of fermentation liquid, in particular to a solid-liquid separation system of fermentation liquid for producing PQQ by a fermentation method, belonging to the technical field of solid-liquid separation in food production.
Background
Pyrroloquinoline quinone (pyrroloquinoline quinone, PQQ) is a novel organic coenzyme different from nicotinamide nucleotides (NAD deca and nadp+) and flavin nucleotides (FAD and FMN), and the ability of PQQ to catalyze redox reactions far exceeds known bioactive molecules to date. In vivo and in vitro studies have shown that PQQ is capable of stimulating microbial growth, enhancing its adaptation to extreme environments, and is important for plant and animal growth, development and reproduction. The production route of PQQ by microbial fermentation is now becoming increasingly accepted as a water-soluble vitamin which is released as an extracellular product into the fermentation broth during fermentation. Because of its structural characteristics, PQQ is liable to undergo addition reaction with a large number of nucleophiles such as amino acids to form imidazole derivatives (1 PQ) or oxazole derivatives (OPQ), and therefore the fermentation broth after the fermentation process is completed is quite unstable in nature and is liable to turn to yellowish green, and the potency is remarkably lost.
However, the solid-liquid separation process in the production process needs longer time, generally exceeds 15 hours, which leads to serious degradation of PQQ and influences the yield. In the prior art, the PQQ is produced by a fermentation method by adopting a solid-liquid separation mode aiming at the first step of the separation and purification process of fermentation liquor, in the prior published journal or patent, the solid-liquid separation treatment modes are numerous,
but some technologies cannot realize scale-up production, for example: CN1329004a (chinese patent, publication No. 2002.01.02), which adopts a solid-liquid separation method that is a centrifugal method. The solid-liquid separation technical route disclosed in CN107056782a (chinese patent, publication date 2017.08.18) is also centrifugation.
Or the problem of color change of the fermentation broth is not solved, for example, the fermentation broth is pretreated by the technical route disclosed in CN111440167A (Chinese patent, publication date 2020.07.24) to obtain filtrate, the pH value of the filtrate is adjusted to 2.5-3.5 by acid, and an adsorbent is added for the first filtration to obtain filtrate; regulating pH value of the filtrate to 7.5-8, adding adsorbent, and filtering for the second time to obtain filtrate; ultrafiltering and nanofiltration are carried out on the filtrate to obtain nanofiltration concentrated solution, the adopted equipment is one of a plate frame, a centrifuge and a ceramic membrane, and the similar process does not essentially solve the problem of color change. The technical route disclosed in CN110698472a (chinese patent, publication date 2020.01.17) uses membrane filtration for solid-liquid separation, but the process requires a large amount of water top washing, the fermentation liquor residence time is too long, and deterioration is easy. The technical route disclosed in CN109180674A (Chinese patent, publication date 2019.01.11) uses membrane filtration to obtain filtrate, the filtrate is adjusted to 4.0-4.3 by acid to remove impurities, the filtrate is stirred for 1-1.5h and then filtered, wherein the filter membrane is precoated with a layer of mesoporous silica, the filtrate is filtered to obtain impurity-removed filtrate, and the first step of membrane filtration of the process is very time-consuming.
At present, the fermentation process of the PQQ in the food industry carries out solid-liquid separation treatment on fermentation liquor, and solid-liquid separation equipment cannot rapidly treat a large amount of fermentation liquor due to the influence of amplification effect. Under the standing state of the fermentation liquor containing high-titer PQQ, pyrroloquinoline quinone disodium salt is easy to carry out addition reaction with a plurality of nucleophilic substances such as amino acid and the like to form imidazole derivatives (1 PQ) or oxazole derivatives (OPQ), so that the fermentation liquor rapidly turns into yellowish green (1-2 h), and the titer loss is serious.
Disclosure of Invention
In order to solve the problems in the prior art, the utility model aims to solve the problem of titer loss in the solid-liquid separation process of mass production of the PQQ fermentation liquid. The solid-liquid separation system of the fermentation liquor comprises a fermentation tank, ceramic membrane equipment and a permeate storage tank; the fermentation tank comprises a fermentation tank body and a fermentation tank jacket; the center of the outer wall of the top of the fermentation tank body is provided with a stirring motor, the outer wall of the top is sequentially connected with a material beating pipeline and a purified water pipeline from the center to the outside, and the edge of the top of the fermentation tank body is provided with an air pipeline; the material beating pipeline is connected to a material feeding port of the top outer wall of the fermentation tank body; the output shaft of the stirring motor is connected with a stirring mechanism arranged in the fermentation tank body through a coupler; the fermentation tank jacket is arranged outside the fermentation tank body and is provided with a chilled water inlet pipeline and a chilled water outlet pipeline; a discharge hole is formed in the bottom of the fermentation tank body; the discharge port is connected to the discharging pump through a pipeline; the discharging pump is connected to the ceramic membrane equipment through a pipeline; the ceramic membrane equipment further comprises a first membrane component and a second membrane component; a variable-frequency circulating pump is arranged at the front end of the first membrane component; the concentrated solution outlets of the first membrane component and the second membrane component are connected and converged through pipelines and then connected to a feed inlet of the outer wall of the top of the fermentation tank body; the permeate outlets of the first membrane component and the second membrane component are connected to the feed inlet of the permeate storage tank through pipelines.
The solid-liquid separation system of the fermentation broth comprises a stirring mechanism, a stirring device and a liquid-liquid separation device, wherein the stirring mechanism comprises a rotating shaft and a stirring paddle; the stirring motor drives the stirring mechanism to stir the feed liquid so as to fully mix the feed liquid.
The solid-liquid separation system of the fermentation liquid comprises a first membrane component and a second membrane component, wherein ceramic membranes are arranged in the first membrane component and the second membrane component.
The solid-liquid separation system of the fermentation liquid is characterized in that a pressure sensor and a pressure gauge are arranged on a pipeline after the concentrated liquid is converged; a pressure sensor is arranged on a pipeline from a permeate outlet of the second membrane assembly to a feed inlet of the permeate storage tank; pressure sensors are arranged on a pipeline behind the discharging pump and a pipeline in front of the variable-frequency circulating pump; ball valves are arranged on the pipelines.
The beneficial technical effects are as follows: according to the utility model, the fermentation tank is transformed to be connected into the concentrated solution loop pipeline of the ceramic membrane equipment, and the circulating tank, the clean water storage tank and the cooling device which are configured by the ceramic membrane equipment are omitted. Compared with the prior ceramic membrane solid-liquid separation process, the configuration of a circulating tank is reduced, and the yield is improved by 14-20%.
Drawings
FIG. 1 is a schematic diagram of a solid-liquid separation system for fermentation broth.
Reference numerals: 1. a purified water line; 2. a material beating pipeline; 3. an air line; 4. a chilled water inlet line; 5. a chilled water outlet line; 6. a stirring motor; 7. a fermenter jacket; 8. a fermenter body; 9. a ball valve; 10. a pressure sensor; 11. a pressure gauge; 12. a discharging pump; 13. a variable frequency circulation pump; 14. a first membrane module; 15. a second membrane module; 16. a permeate storage tank; 17. and a stirring mechanism.
Detailed Description
The present utility model is further illustrated by the following examples, but is not limited thereto.
As shown in fig. 1, a solid-liquid separation system of fermentation liquid comprises a fermentation tank, ceramic membrane equipment and a permeate storage tank 16; the fermentation tank comprises a fermentation tank body 8 and a fermentation tank jacket 7; the center of the outer wall of the top of the fermentation tank body 8 is provided with a stirring motor 6, the outer wall of the top is sequentially connected with a material beating pipeline 2 and a purified water pipeline 1 from the center to the outside, and the edge of the top of the fermentation tank body 8 is provided with an air pipeline 3; the material beating pipeline 2 is connected to a material feeding port of the top outer wall of the fermentation tank body 8; the output shaft of the stirring motor 6 is connected with a stirring mechanism 17 arranged in the fermentation tank body 8 through a coupler; wherein the stirring mechanism 17 comprises a rotating shaft and a stirring paddle; the stirring motor 6 drives the stirring mechanism 17 to stir the feed liquid so as to fully mix the feed liquid. The fermentation tank jacket 7 is arranged outside the fermentation tank body 8 and is provided with a chilled water inlet pipeline 4 and a chilled water outlet pipeline 5; a discharge hole is formed in the bottom of the fermentation tank body 8; the discharge port is connected to the discharge pump 12 through a pipeline; the discharge pump 12 is connected to the ceramic membrane device by a pipe.
The ceramic membrane equipment comprises a first membrane component 14 and a second membrane component 15, wherein a ceramic membrane is arranged; a variable frequency circulating pump 13 is arranged at the front end of the first membrane component 14; the concentrated solution outlets of the first membrane component 14 and the second membrane component 15 are connected and converged through pipelines and then connected to a feed inlet of the top outer wall of the fermentation tank body 8; wherein, a pressure sensor 10 and a pressure gauge 11 are arranged on a pipeline after the concentrated solutions are converged.
The permeate outlets of the first membrane module 14 and the second membrane module 15 are connected to the feed inlet of the permeate storage tank 16 through pipelines; the pressure sensor 10 is disposed on a pipeline from a permeate outlet of the second membrane module 15 to a feed port of the permeate storage tank 16. The pipeline behind the discharging pump 12 and the pipeline in front of the variable frequency circulating pump 13 are respectively provided with a pressure sensor 10; and ball valves 9 are arranged on the pipelines. Therefore, a user can monitor the pressure condition of each important pipeline and can independently control the on-off of the pipeline through each ball valve 9.
The operation process of the solid-liquid separation system of the fermentation liquid is as follows:
after the fermentation state is finished, introducing chilled water into a coil pipe of a fermentation tank jacket 7, and cooling to 10-20 ℃; the air flow is regulated to 0.5-1.0VVM; the stirring rotation speed is started to be 10-20HZ so as to ensure that the dissolved oxygen and the temperature are uniformly distributed; and starting ceramic membrane equipment to start circulating filtration, and adding purified water to circulate and top-wash for 3 times when the volume of fermentation liquor is concentrated to 1/5-1/10 of the original volume in the treatment process.
The utility model reduces the configuration of the ceramic membrane equipment circulating tank by reconstruction, uses the fermentation tank as the circulating tank, maintains the stability of the fermentation liquor property by continuously ventilating and cooling, and improves the solid-liquid separation yield of the PQQ. Three batches of test production are respectively carried out by comparing the process yield of the ceramic membrane of the fermentation tank of 10 tons before transformation with the process yield of the ceramic membrane after transformation.
The technology before improvement is that after the fermentation process is finished, the fermentation liquor is transferred to a circulating storage tank, and is filtered and circulated through a ceramic membrane, and when the fermentation liquor is concentrated to 1/5-1/10 of the original volume, purified water is added into the storage tank for top washing, and the top washing volume is 3 times of that of the fermentation liquor.
After the fermentation state of the improved process is finished, cooling the jacket to 10-20 ℃ by introducing chilled water; the air flow is regulated to 0.5-1.5VVM; the stirring rotation speed is started to be 10-20HZ so as to ensure that the dissolved oxygen and the temperature are uniformly distributed; and starting ceramic membrane equipment to start circulating filtration, and adding purified water to circulate and top-wash for 3 times when the volume of fermentation liquor is concentrated to 1/5-1/10 of the original volume in the treatment process. The yield of the ceramic membrane solid-liquid separation process PQQ is compared before and after improvement, and the yield of the process is improved by 14-20% after improvement.
The foregoing is merely a preferred embodiment of the present patent, and it should be noted that modifications and substitutions will now occur to those skilled in the art without departing from the technical principles of the present patent, and such modifications and substitutions should also be considered to be within the scope of the present patent.
Claims (5)
1. A solid-liquid separation system of fermentation liquid comprises a fermentation tank, ceramic membrane equipment and a permeate storage tank (16); the method is characterized in that: the fermentation tank comprises a fermentation tank body (8) and a fermentation tank jacket (7);
the center of the outer wall of the top of the fermentation tank body (8) is provided with a stirring motor (6), the outer wall of the top is sequentially connected with a material beating pipeline (2) and a purified water pipeline (1) from the center to the outside, and the edge of the top of the fermentation tank body (8) is provided with an air pipeline (3); the material beating pipeline (2) is connected to a material feeding port of the top outer wall of the fermentation tank body (8);
an output shaft of the stirring motor (6) is connected with a stirring mechanism (17) arranged in the fermentation tank body (8) through a coupler;
the fermentation tank jacket (7) is arranged outside the fermentation tank body (8) and is provided with a chilled water inlet pipeline (4) and a chilled water outlet pipeline (5);
a discharge hole is formed in the bottom of the fermentation tank body (8); the discharge port is connected to a discharging pump (12) through a pipeline; the discharging pump (12) is connected to the ceramic membrane equipment through a pipeline;
the ceramic membrane device comprises a first membrane component (14) and a second membrane component (15); a variable frequency circulating pump (13) is arranged at the front end of the first membrane component (14);
the concentrated solution outlets of the first membrane component (14) and the second membrane component (15) are connected and converged through pipelines and then connected to a feed inlet of the top outer wall of the fermentation tank body (8);
the permeate outlets of the first membrane component (14) and the second membrane component (15) are connected to the feed inlet of the permeate storage tank (16) through pipelines.
2. The solid-liquid separation system of fermentation broth according to claim 1, wherein the stirring mechanism (17) further comprises a rotating shaft and a stirring paddle; the stirring motor (6) drives the stirring mechanism (17) to stir the feed liquid so as to fully mix the feed liquid.
3. The solid-liquid separation system of fermentation broth according to claim 1, wherein ceramic membranes are provided in the first membrane module (14) and the second membrane module (15).
4. The solid-liquid separation system of fermentation broth according to claim 1, wherein a pressure sensor (10) and a pressure gauge (11) are arranged on the pipeline after the concentrated broth is converged; a pressure sensor (10) is arranged on a pipeline from a permeate outlet of the second membrane component (15) to a feed inlet of the permeate storage tank (16); pressure sensors (10) are arranged on a pipeline behind the discharging pump (12) and a pipeline in front of the variable-frequency circulating pump (13).
5. The solid-liquid separation system of fermentation broth according to claim 4, wherein each of the pipelines is provided with a ball valve (9).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202222893628.1U CN219010270U (en) | 2022-10-31 | 2022-10-31 | Solid-liquid separation system of fermentation broth |
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| CN202222893628.1U CN219010270U (en) | 2022-10-31 | 2022-10-31 | Solid-liquid separation system of fermentation broth |
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| CN219010270U true CN219010270U (en) | 2023-05-12 |
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| CN202222893628.1U Active CN219010270U (en) | 2022-10-31 | 2022-10-31 | Solid-liquid separation system of fermentation broth |
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