CN115581274A - Preparation method of prepared celery powder - Google Patents
Preparation method of prepared celery powder Download PDFInfo
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- CN115581274A CN115581274A CN202211228455.XA CN202211228455A CN115581274A CN 115581274 A CN115581274 A CN 115581274A CN 202211228455 A CN202211228455 A CN 202211228455A CN 115581274 A CN115581274 A CN 115581274A
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- URQMEZRQHLCJKR-UHFFFAOYSA-N 3-Methyl-5-propyl-2-cyclohexen-1-one Chemical compound CCCC1CC(C)=CC(=O)C1 URQMEZRQHLCJKR-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 238000000855 fermentation Methods 0.000 claims abstract description 46
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 238000001914 filtration Methods 0.000 claims abstract description 29
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- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 16
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- 239000012528 membrane Substances 0.000 claims description 65
- 239000000919 ceramic Substances 0.000 claims description 25
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- 238000001694 spray drying Methods 0.000 claims description 14
- 238000005406 washing Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 7
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 239000005913 Maltodextrin Substances 0.000 claims description 2
- 229920002774 Maltodextrin Polymers 0.000 claims description 2
- 239000005557 antagonist Substances 0.000 claims description 2
- 239000013505 freshwater Substances 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 229940035034 maltodextrin Drugs 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
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- 239000012141 concentrate Substances 0.000 claims 2
- 239000011550 stock solution Substances 0.000 claims 1
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- 238000007599 discharging Methods 0.000 abstract 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 31
- 238000002834 transmittance Methods 0.000 description 21
- 230000008569 process Effects 0.000 description 12
- 230000004907 flux Effects 0.000 description 10
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- 239000001888 Peptone Substances 0.000 description 2
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- 241000196324 Embryophyta Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000212324 Oenanthe <angiosperm> Species 0.000 description 1
- 240000002834 Paulownia tomentosa Species 0.000 description 1
- 235000010678 Paulownia tomentosa Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
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- DGLRDKLJZLEJCY-UHFFFAOYSA-L disodium hydrogenphosphate dodecahydrate Chemical compound O.O.O.O.O.O.O.O.O.O.O.O.[Na+].[Na+].OP([O-])([O-])=O DGLRDKLJZLEJCY-UHFFFAOYSA-L 0.000 description 1
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- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
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- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
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- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
The invention belongs to the technical field of extraction engineering technology, and relates to a preparation method of prepared celery powder, which comprises the following steps: (1) Preparing fermentation liquor, namely taking concentrated celery juice as a fermentation raw material, inoculating a strain, and fermenting to obtain celery juice fermentation liquor; (2) Pretreating the fermentation liquor, namely performing solid-liquid separation by adopting a live bacteria tank-discharging mode to remove thalli and impurities; (3) Decoloring and purifying the fermentation liquor, namely filtering the pretreated fermentation liquor by adopting an ultrafiltration membrane filtration mode to remove pigments, foreign proteins and high molecular impurities; (4) Mixing celery powder, adding auxiliary materials into the filtrate, and uniformly stirring to obtain a prepared material; (5) Drying, drying the materials by a drying device, and adding an anticaking agent in the drying process to obtain the prepared celery powder. The invention provides a method for extracting and preparing prepared celery powder with the functions of preservation, preservation and food flavor improvement from concentrated celery juice leavening in a green, stable and efficient manner.
Description
Technical Field
The invention belongs to the technical field of extraction engineering technology, and relates to a preparation method for preparing celery powder.
Background
Fermented celery powder is a clean label product extracted by plant fermentation. Compared with the nitrite synthesized by the traditional chemistry, the components of vitamin C, polyphenol substances and the like contained in the vegetables can react with the nitrite to reduce the nitrite residue. The fermented celery powder can effectively improve the flavor and the quality of food in function, has good application effects in the aspects of pickling and freshness improvement of meat products, flavor improvement, cooking seasoning, freshness preservation and corrosion prevention, particularly has unique flavor of the fermentation of the celery powder, and has unique effect in the aspect of improving the taste of the food. Therefore, the fermented celery powder serving as a green clean label product has high popularization value in the international market.
Up to now, the main literature on the extraction and preparation of celery powder published in the field is:
1. CN 201310384941A preparation process, product and application of herba Oenanthes Javanicae powder is a patent applied by Tung City bull and cattle agriculture development Limited, and comprises the following steps (1); (2) cleaning; (3) secondary cleaning; (4) disinfection and color protection; (5) drying; (6) crushing; and (7) packaging and warehousing.
2. A study on the preparation of fermented celery powder and the application effect in clysis, which is made by the academic thesis of Tianjin academy of agriculture, describes a method for preparing fermented vegetable powder with high content of natural nitrite. In this paper, the nitrite content can be significantly increased by adjusting the pH to 7.0 at intervals during the fermentation process.
3. The master academic thesis of south Jiangnan university, "Key production technology of Mirabilitum-rich celery powder and application thereof in meat products", discloses an optimal pH range in the fermentation process: 7.0-7.2, fermentation temperature: 37 ℃, fermentation time: 10h; and after the secondary inoculation method is adopted, the nitrate conversion rate is increased from 60.66% to 89.01%.
4. The patent number of CN201510719155.5, a preparation method of celery powder capsules, the invention mainly comprises four steps of celery treatment, celery leaf preparation, celery powder preparation and finished product preparation.
5. The patent numbers are: CN201510762357.8, a method for preparing celery powder, the invention patent of inventor Zhang Yu application, the patent mainly comprises the steps of cleaning celery, soaking and scalding with hot water, beating into serous fluid in a stirrer, filtering the serous fluid, spraying into celery powder by a spray dryer, and finally hermetically packaging the celery powder to obtain the finished celery powder tablet.
As described above, although the research and production are carried out by the existing institutions, the used raw materials are celery, the celery is not fermented, the extraction process is only simple homogenate drying and mixing, and the large-scale production is not realized for a while. Therefore, a set of extraction and purification process which is green and stable and suitable for industrial production is designed, the prior art is broken through, and the improvement of the quality of the celery powder becomes a technical problem which needs to be solved urgently in the field.
Disclosure of Invention
Aiming at the difference of the prior art, the invention provides a method for extracting and preparing the prepared celery powder with the functions of preservation, preservation and food flavor improvement from the concentrated celery juice fermentation product in a green, stable and efficient manner.
In order to solve the above technical problems, the object of the present invention is achieved by the following technical solutions:
a method of preparing a formulated celery powder, the method comprising the steps of:
(1) Preparing fermentation liquor, namely taking concentrated celery juice as a fermentation raw material, inoculating a strain, and fermenting to obtain celery juice fermentation liquor;
(2) Pretreating the fermentation liquor, namely performing solid-liquid separation by adopting a live bacteria tank-placing mode and controlling the temperature to be not more than 50 ℃ to remove thalli and impurities;
(3) Decoloring and purifying the fermentation liquor, namely filtering the fermentation liquor pretreated in the step (2) by adopting an ultrafiltration membrane at the temperature not higher than 35 ℃ to remove pigments, foreign proteins and high molecular impurities;
(4) Mixing the celery powder, adding auxiliary materials into the filtrate obtained in the step (3), and uniformly stirring to obtain a prepared material;
(5) And (4) drying, namely drying the material obtained in the step (4) by using drying equipment, and adding an anticaking agent in the drying process to obtain the prepared celery powder.
In the preparation method for preparing the celery powder, the purpose of the bacterial liquid separation in the step (2) is to separate thalli and insoluble substances in the fermentation liquor to obtain clear and transparent dark brown liquid, and the solid-liquid separation in the step (2) is selected from ceramic membrane filtration, plate-and-frame filtration or centrifugal separation.
In the preparation method for preparing the celery powder, preferably, the solid-liquid separation method in the step (2) is ceramic membrane filtration, and the pore diameter of the ceramic membrane is 50nm.
In the preparation method for preparing the celery powder, the decoloring and purifying in the step (3) are carried out by passing liquid obtained by bacteria liquid separation through an ultrafiltration membrane or a nanofiltration membrane, and taking ultrafiltration permeate with light color and less impurities, wherein the molecular weight of the ultrafiltration membrane in the step (3) is 300-5000.
In the preparation method for preparing the celery powder, preferably, the molecular weight of the ultrafiltration membrane in the step (3) is 1000-2000.
In the preparation method for preparing the celery powder, solid-liquid separation is carried out in the step (2) in a filtering mode, when the volume percentage of a filtering liquid reaches 90% during filtering, fresh water is added and washed, the adding speed is consistent with the filtering speed, and filtering is finished after the volume of the filtering liquid is equal to that of a raw liquid; and (4) when the ultrafiltration membrane is used for filtering in the step (3), when the volume of the filtrate reaches 90%, starting to perform flow addition washing of clear water, wherein the flow addition speed is consistent with the filtering speed, and filtering is finished after the volume of the filtered liquid is equal to that of the original liquid.
In the preparation method for preparing the celery powder, in the step (4), the adding amount of the auxiliary materials is calculated according to the product requirements of different specifications, and the auxiliary materials are added into the ultrafiltration permeating liquid, wherein the auxiliary materials in the step (4) are selected from one or more of sodium chloride, potassium chloride, maltodextrin and glucose. The nitrite content includes various specifications such as 1000ppm to 100000pmm, and the approximate specifications can be classified into nitrite contents of 1000ppm,5000ppm,10000ppm,20000ppm,30000ppm,50000ppm,100000ppm, etc.
The calculation formula of the auxiliary materials is as follows: in the formula of M1= (V1 xC 1) ÷ C2-M2 xT 1, M1 is the auxiliary material mass, V1 is the decoloring liquid volume, C1 is the decoloring liquid nitrite content, M2 is the decoloring liquid mass, T1 is the liquid dry matter, and C2 is the target celery powder nitrite content percentage.
In the preparation method for preparing the celery powder, the antagonist in the step (5) is silicon dioxide, and preferably, the addition amount of the silicon dioxide is 2% of the mass of the target celery powder.
In the preparation method for preparing the celery powder, the drying equipment in the step (5) is pressure type spray drying equipment or centrifugal type spray drying equipment, when the pressure type spray drying equipment is used for drying, the air inlet temperature is set to be 180-220 ℃, the air outlet temperature is set to be 100-120 ℃, and the atomization pressure is set to be 100Mpa; when centrifugal spray drying equipment is used for drying, the air inlet temperature is set to be 220-240 ℃, the air outlet temperature is set to be 100-140 ℃, and the atomization frequency is set to be 3500r/m.
In the preparation method for preparing the celery powder, preferably, the celery juice fermentation liquor is filtered by ceramic membrane equipment, the volume of the celery juice fermentation liquor is concentrated by 10 times, the thallus is washed and concentrated by one time, the ceramic membrane permeate is decolorized and purified by an ultrafiltration membrane with the molecular weight of 2000, the volume of the ceramic membrane permeate is concentrated by 10 times, the volume of the ceramic membrane permeate is washed and concentrated by 1 time, the ultrafiltration permeate is taken, auxiliary materials are added, the mixture is uniformly stirred, and the mixture is sprayed and dried by pressure type spray drying equipment to obtain the powdery fermented celery powder.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a method for stably and efficiently producing celery powder rich in nitrite. Concentrated celery juice is used as a fermentation raw material, the average content of nitrite in the fermentation liquor reaches more than 10000ppm, a large amount of impurities in the fermentation liquor are removed through a green, stable and easily-industrialized extraction and purification process, the color and flavor of the product are obviously improved, no chemical reagent is added in the separation and purification process, the green and clean concept is met, and the total extraction yield reaches more than 90%.
2. The extraction process provided by the invention not only can effectively separate the pigment impurities of thalli, but also can better retain the nutrient components in the fermentation liquor, and no chemical reagent is added in the extraction process, so that the process industrialization condition is mature.
3. The invention adopts a secondary filtration technology and selects a proper filtration process, so that various impurities and pigments can be removed to the maximum extent, and the obtained product has higher purity. Meanwhile, the invention adopts a low-temperature filtration mode, so that the nutrient components of the raw materials can be better reserved.
4. In the traditional preparation process of preparing the celery powder, the celery powder is generally prepared firstly, and then the celery powder and the required auxiliary material powder are uniformly mixed, but the traditional preparation method of preparing the celery powder is changed. In the production process of the celery powder, auxiliary materials are added, and the celery powder is firstly mixed, dissolved and uniformly stirred and then is dried to generate integrated powder. The prepared celery powder has higher uniformity and better quality.
Drawings
FIG. 1 is a graph comparing the instantaneous flux of the various separation modes of the examples;
FIG. 2 is a graph showing the comparison of the average yields obtained by ultrafiltration membranes of different membrane molecular weights;
FIG. 3 is a graph showing the comparison of the decolorization effect of ultrafiltration membranes of different membrane molecular weights.
FIG. 4 is a graph showing the average flux in ultrafiltration membranes of different membrane molecular weights.
Detailed Description
The present invention is further described by the following description of the embodiments, which are not intended to limit the invention, and those skilled in the art can make various modifications or improvements based on the basic idea of the invention, but within the scope of the invention, without departing from the basic idea of the invention.
The starting materials and reagents used in the following examples were all commercially available unless otherwise specified.
(1) Preparation of fermentation broth
Example 1
Seed medium (%):
0.5 percent of yeast powder; 0.3% of peptone; 0.2 percent of sodium chloride.
Fermentation medium (%):
30% of celery juice; 1.0% of yeast extract powder; 0.5% of peptone; 0.5 percent of sodium chloride; disodium hydrogen phosphate dodecahydrate 0.05%.
The prepared glycerol pipecolin suspension is unfrozen, transferred into a 500mL triangular flask containing 100mL seed culture medium, and cultured for about 15h by shaking at 37 ℃ and 150 r/min.
The cultured seed solution was inoculated in a 100L seed tank containing 70L of seed medium in an amount of 0.5% and cultured with stirring. Inoculating the cultured seed solution into a 700L mechanical stirring type fermentation tank with the inoculation amount of 10%, wherein the initial pH is 7.0, and inoculating in two stages: in the stage I, the cultured seed liquid is transferred into a fermentation medium for culture, and the pH is controlled to be beneficial to the proliferation of thalli; in the stage II, when the fermentation culture is carried out for 5 hours, the cultured seed liquid is inoculated for the second time, the pH is continuously controlled, and liquid caustic soda is automatically fed to maintain the pH at about 7.0, so as to be beneficial to the growth of thalli and the conversion of products; during the fermentation, the temperature was controlled at 37 ℃.
(2) Pretreatment of fermentation broth
Example 2
Taking 500L of celery juice fermentation liquor, wherein the nitrite content is 10866ppm, filtering the fermentation liquor by using a ceramic membrane, controlling the aperture of a membrane core to be 50nm, the membrane area to be 3 square meters, controlling the operation temperature to be about 40 ℃, concentrating the solution by 10 times of the volume, washing the thalli by one time of the volume, collecting the filtrate by 500L totally, wherein the nitrite content is 10648, and the yield is 98%.
Example 3
Taking 500L of celery juice fermentation liquor, wherein the content of nitrite is 10765ppm, filtering the fermentation liquor with a plate frame, wherein the filtering area is 3 square meters, circularly cleaning for 10min with 50L of clean water after the feeding is finished, squeezing and drying, and collecting the filtrate for 520L, wherein the content of nitrite is 10268ppm, and the yield is 99.3%.
Example 4
Taking 500L of celery juice fermentation liquor, wherein the nitrite content is 11245ppm, performing bacteria liquid separation by using a three-legged centrifuge at the rotation speed of 35000rad/min, washing the materials with 50L of clean water after the materials are fed, and collecting 505L of filtrate, wherein the nitrite content is 10521ppm, and the yield is 94.6%.
Through tests, the three devices can effectively separate thalli and solid matters, wherein the yield of the centrifugal mode is slightly low, and the influence of the three coarse filtration modes on the subsequent decolorization and purification of the ultrafiltration membrane is further tested.
(3) Decolorization and purification of fermentation broth
In examples 5 to 7, the influence of the three separation methods in step (2) on the decoloring and purifying processes of the ultrafiltration membrane was examined, the ultrafiltration membrane equipment used was equipment provided for Jiu' an source, the ultrafiltration membrane used was a 2000-molecular roll-type membrane, the membrane specification was 2540, and the membrane area was 0.25m 2 。
Examples 8 to 14 were conducted to test the molecular weight types of ultrafiltration membranes in the decolorization and purification step, wherein the ultrafiltration membrane equipment used was a Hangzhou Rener experimental ultrafiltration membrane equipment, the membrane specification was 1812, and the molecular weights of the membranes were selected from 150, 300, 500, 800, 1000, 2000, and 5000, respectively.
Example 5
Taking 100L of the ceramic membrane filtrate obtained in example 2, wherein the nitrite content is 10284ppm, the initial transmittance is 25.6%, passing through an ultrafiltration membrane, the inlet pressure is 1.5mpa, the operating temperature is controlled below 35 ℃, the ceramic membrane filtrate is concentrated by 10 times of volume, the ceramic membrane filtrate is washed by 1 time of volume, the total amount of permeate is 100L, the content is 10078ppm, the transmittance at 550nm wavelength is 86.5%, and the yield is 98%, and recording the operation condition of the equipment.
Example 6
Taking 100L of plate-frame filtrate in example 3, wherein the nitrite content is 10356ppm, the initial transmittance is 24.9%, passing through an ultrafiltration membrane, the inlet pressure is 1.5mpa, the operation temperature is controlled below 35 ℃, the plate-frame filtrate is concentrated by 10 times of volume, washed by 1 time of the volume of the concentrated solution, the total amount of permeate is 100L, the content is 10128ppm, the transmittance at the wavelength of 550nm is 86.2%, and the yield is 97.8%, and recording the operation condition of equipment.
Example 7
100L of the supernatant from example 4, which contained 10284ppm nitrite and had an initial transmittance of 25.2%, was passed through an ultrafiltration membrane at an inlet pressure of 1.5mpa and an operating temperature of 35 ℃ or below, was concentrated 10 times by volume, washed 1 time by volume, and 100L of permeate was measured to contain 10109ppm nitrite and have a transmittance of 86.5% at 550nm with a yield of 98.3%, and the operating conditions of the apparatus were recorded.
Tests show that the three filtrates are decolorized and purified by an ultrafiltration membrane, the transmittance and the yield are very close, and the integral flux of the decolorization process of the ceramic membrane filtrate is obviously superior to that of plate-frame filtrate and centrifugal filtrate, so that the ceramic membrane filtration is preferably used as a filtration mode for bacteria liquid separation.
Example 8
Taking 10L of ceramic membrane filtrate, wherein the nitrite content is 10842ppm, the initial transmittance is 24.6%, decolorizing and purifying with ultrafiltration membrane with molecular weight of 150, setting inlet pressure to be 1.5mpa, controlling operation temperature below 35 ℃, concentrating by 10 times of volume, washing by 1 time of concentrated solution volume, measuring the total volume of permeate liquid to be 10L, measuring the content to be 9757ppm, the transmittance at 550nm wavelength to be 94.2%, the process yield is 90%, and the average flux is 15.6L/h x m 2 。
Example 9
Taking 10L of ceramic membrane filtrate, wherein nitrite content is 10842ppm, initial transmittance is 24.6%, decolorizing and purifying with ultrafiltration membrane with molecular weight of 300, inlet pressure is set to 1.5mpa, operation temperature is controlled below 35 ℃, concentrating by 10 times volume, washing by 1 time volume of concentrated solution, total 10L of permeate, measuring content to be 10083ppm, transmittance at 550nm wavelength is 92.5%, process yield is 94.8%, average flux is 18.3L/h m 2 。
Example 10
Taking 10L of ceramic membrane filtrate, wherein the nitrite content is 10842ppm, the initial transmittance is 24.6%, decolorizing and purifying with ultrafiltration membrane with molecular weight of 500, setting inlet pressure to be 1.5mpa, controlling operation temperature below 35 ℃, concentrating by 10 times of volume, washing by 1 time of concentrated solution volume, measuring the total amount of permeate liquid to be 10L, measuring the content to be 10473ppm, the transmittance at 550nm wavelength to be 90.2%, the process yield is 96.6%, and the average flux is 20.6L/h m 2 。
Example 11
Taking 10L of ceramic membrane filtrate, wherein the nitrite content is 10842ppm, the initial transmittance is 24.6%, decolorizing and purifying with ultrafiltration membrane with molecular weight of 800, setting inlet pressure to be 1.5mpa, controlling operation temperature below 35 ℃, concentrating by 10 times of volume, washing by 1 time of concentrated solution volume, measuring the total volume of permeate liquid to be 10L, measuring the content to be 10570ppm, the transmittance at wavelength of 550nm to be 86.2%, the process yield is 97.5%, and the average flux is 21.6L/h x m 2 。
Example 12
Taking 10L of ceramic membrane filtrate, wherein the nitrite content is 10842ppm, the initial transmittance is 24.6%, decolorizing and purifying with ultrafiltration membrane with molecular weight of 1000, setting inlet pressure at 1.5mpa, controlling operation temperature below 35 ℃, concentrating by 10 times volume, washing by 1 time volume of concentrated solution, measuring the total volume of permeate liquid by 10L, measuring the content to be 10711ppm, the transmittance at wavelength of 550nm is 84.6%, the process yield is 98.8%, and the average flux is 23.9L/h m 2 。
Example 13
Taking 10L of ceramic membrane filtrate, wherein nitrite content is 10842ppm, initial transmittance is 24.6%, and decolorizing with ultrafiltration membrane having molecular weight of 2000Dissolving at inlet pressure of 1.5mpa, operating temperature below 35 deg.C, concentrating by 10 times volume, washing by 1 time volume of concentrated solution, measuring total 10L of permeate, its content is 10668ppm, transmittance at 550nm wavelength is 82.3%, process yield is 98.4%, and average flux is 28.6L/h m 2 。
Example 14
Taking 10L of ceramic membrane filtrate, wherein the nitrite content is 10842ppm, the initial transmittance is 24.6%, decolorizing and purifying with ultrafiltration membrane with molecular weight of 5000, setting inlet pressure at 1.5mpa, controlling operation temperature below 35 deg.C, concentrating by 10 times volume, washing by 1 time volume of concentrated solution, measuring the total volume of permeate liquid by 10L, measuring the content to be 10755ppm, transmittance at wavelength of 550nm to be 73.9%, process yield to be 99.2%, and average flux to be 39.4L/h m 2 。
After testing and comparative analysis, the conclusion can be drawn: the selected membranes with different molecular weights can achieve certain decolorizing and purifying effects, and the membranes with the molecular weight of 1000-2000 are preferred in consideration of treatment period, yield and decolorizing effect.
(4) Ingredients of celery powder
Example 15
And (4) adding auxiliary materials into the filtrate obtained in the step (3), and uniformly stirring to obtain a prepared material. The configuration scheme of the material is established according to the actual nitrite content of the product and the requirement of auxiliary materials, the nitrite content comprises different specifications of 1000 ppm-100000 pmm, and the approximate specifications can be divided into nitrite content of 1000ppm,5000ppm,10000ppm,20000ppm,30000ppm,50000ppm,100000ppm and the like.
(5) Drying
Example 16
And drying the prepared material by using centrifugal spray drying equipment, wherein the air inlet temperature is 220-240 ℃, the air outlet temperature is 100-140 ℃, the atomization frequency is set to 3500r/m, and 2% of silicon dioxide is quantitatively added in the drying process to obtain powdery fermented celery powder. The test shows that the mass yield is 95%, the content yield is 100% and the water content is 2% in the centrifugal spray drying process.
Example 17
Drying the prepared materials by using pressure type spray drying equipment, wherein the air inlet temperature is 180-220 ℃, the air outlet temperature is 100-120 ℃, and the atomization pressure is set to be 100Mpa, so that the powdery fermented celery powder is obtained. The test pressure type spray drying process shows that the mass yield is 98%, the content yield is 100% and the water content is 2.5%.
Claims (10)
1. A preparation method of prepared celery powder is characterized by comprising the following steps:
(1) Preparing fermentation liquor, namely taking concentrated celery juice as a fermentation raw material, inoculating a strain, and fermenting to obtain celery juice fermentation liquor;
(2) Pretreating the fermentation liquor, namely performing solid-liquid separation by adopting a live bacteria tank-placing mode and controlling the temperature to be not more than 50 ℃ to remove thalli and impurities;
(3) Decoloring and purifying the fermentation liquor, namely filtering the fermentation liquor pretreated in the step (2) by adopting an ultrafiltration membrane at the temperature not higher than 35 ℃ to remove pigments, foreign proteins and high molecular impurities;
(4) Mixing celery powder, adding auxiliary materials into the filtrate obtained in the step (3), and uniformly stirring to obtain a prepared material;
(5) And (4) drying, namely drying the material obtained in the step (4) by using drying equipment, and adding an anticaking agent in the drying process to obtain the prepared celery powder.
2. The method for preparing formulated celery powder as claimed in claim 1, wherein the solid-liquid separation in step (2) is selected from ceramic membrane filtration, plate-and-frame filtration or centrifugal separation.
3. The preparation method of formulated celery powder as claimed in claim 1, wherein the solid-liquid separation method in step (2) is ceramic membrane filtration, and the pore size of the ceramic membrane is 50nm.
4. The preparation method of the formulated celery powder as claimed in claim 1, wherein the ultrafiltration membrane in the step (3) has a molecular weight of 300-5000.
5. The method for preparing formulated celery powder according to claim 1, wherein the ultrafiltration membrane in the step (3) has a molecular weight of 1000-2000.
6. The preparation method for preparing celery powder as claimed in claim 1, wherein solid-liquid separation is performed in the step (2) by filtration, when the volume of the filtrate reaches 90%, fresh water feeding washing is started, the feeding speed is consistent with the filtration speed, and the filtration is finished after the volumes of the filtrate and the stock solution are equal; and (4) when the ultrafiltration membrane is used for filtering in the step (3), when the volume of the filtrate reaches 90%, starting to perform flow addition washing of clear water, wherein the flow addition speed is consistent with the filtering speed, and filtering is finished after the volume of the filtered liquid is equal to that of the original liquid.
7. The method for preparing formulated celery powder as claimed in claim 1, wherein the auxiliary materials in the step (4) are selected from one or more of sodium chloride, potassium chloride, maltodextrin and glucose.
8. The method of claim 1, wherein the antagonist of step (5) is silica.
9. The preparation method for preparing the formulated celery powder as claimed in claim 1, wherein the drying device of the step (5) is a pressure type spray drying device or a centrifugal type spray drying device, when the pressure type spray drying device is used for drying, the inlet air temperature is set to be 180-220 ℃, and the outlet air temperature is set to be 100-120 ℃; when centrifugal spray drying equipment is used for drying, the air inlet temperature is set to be 220-240 ℃, and the air outlet temperature is set to be 100-140 ℃.
10. The preparation method of the prepared celery powder according to claim 1, wherein celery juice fermentation liquor is taken, filtered by a ceramic membrane device, concentrated by 10 times in volume, washed by water to concentrate thalli by one time in volume, a ceramic membrane permeate is subjected to decolorization and purification by an ultrafiltration membrane with the molecular weight of 2000, concentrated by 10 times in volume by ultrafiltration, washed by water to concentrate by 1 time in volume, an ultrafiltration permeate is taken, auxiliary materials are added, the mixture is stirred uniformly, and the mixture is sprayed and dried by a pressure type spray drying device to obtain the powdery fermented celery powder.
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| CN117481326A (en) * | 2023-10-23 | 2024-02-02 | 浙江溢滔食品技术有限公司 | Preparation method of solid seasoning with antibacterial and fresh-keeping functions |
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| CN102224910A (en) * | 2011-05-17 | 2011-10-26 | 江南大学 | Method for producing food ingredient rich in nitrite and flavonoid by use of celery leaves |
| CN103288932A (en) * | 2013-06-13 | 2013-09-11 | 天津科技大学 | Method for separating and extracting pediocin PA-1 from fermentation solution |
| CN104082699A (en) * | 2014-06-16 | 2014-10-08 | 天津农学院 | Preparation method of fermented celery powder based on natural preserving |
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| CN102224910A (en) * | 2011-05-17 | 2011-10-26 | 江南大学 | Method for producing food ingredient rich in nitrite and flavonoid by use of celery leaves |
| CN103288932A (en) * | 2013-06-13 | 2013-09-11 | 天津科技大学 | Method for separating and extracting pediocin PA-1 from fermentation solution |
| CN104082699A (en) * | 2014-06-16 | 2014-10-08 | 天津农学院 | Preparation method of fermented celery powder based on natural preserving |
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| CN117481326A (en) * | 2023-10-23 | 2024-02-02 | 浙江溢滔食品技术有限公司 | Preparation method of solid seasoning with antibacterial and fresh-keeping functions |
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