CN218995399U

CN218995399U - Protein blotting detection kit

Info

Publication number: CN218995399U
Application number: CN202320004590.XU
Authority: CN
Inventors: 田园
Original assignee: Guangdong Medical University
Current assignee: Guangdong Medical University
Priority date: 2023-01-03
Filing date: 2023-01-03
Publication date: 2023-05-09
Anticipated expiration: 2033-01-03

Abstract

The utility model belongs to the technical field of Western blotting kits, and particularly relates to a kit for detecting a protein blotting method, which comprises the following components: a bottom plate with an opening at the top; the groove is arranged in the opening and positioned at the top of the bottom plate and is used for placing the detection film; the sealing mechanism comprises a cover plate which is arranged above the groove and can be pulled in the opening, and sealing strips which are symmetrically arranged at two symmetrical ends of the bottom plate.

Description

Protein blotting detection kit

Technical Field

The utility model belongs to the technical field of Western blotting kits, and particularly relates to a kit for detecting a protein blotting method.

Background

The Western blotting kit is a method of transferring a protein onto a membrane and then detecting by using an antibody; for known expressed proteins, the corresponding antibodies can be used as primary antibodies, and the expression products of the novel genes can be detected by antibodies to the fusion moiety.

Western blotting is a technique similar to n } A zebra method for transferring egg matter from gel, and its basic principle is to color the gel electrophoresis treated cell or biological tissue sample by specific antibody; information on the expression of a specific protein in the analyzed cell or tissue is obtained by analyzing the position of staining and the depth of staining.

The method comprises the following steps: protein extraction, concentration measurement, sample preparation, electrophoresis, membrane transfer, sealing, primary antibody, secondary antibody and exposure; wherein, the first antibody and the second antibody are incubated, no related instrument can be well assisted, and the following problems are solved: a small amount of liquid cannot cover the whole membrane, especially a membrane with a large area;

unsealing during long incubation time can lead to volatilization of liquid and drying of the film; there may be contamination and insufficient recovery and recycling of the antibody solution.

Disclosure of Invention

The utility model aims to provide a protein blotting detection kit for solving the problems that a small amount of liquid cannot cover the whole membrane, the unsealed liquid can volatilize when the membrane is incubated for a long time, and the membrane is dried; there may be a problem in that the antibody solution is contaminated and cannot be sufficiently recovered and recycled.

In order to achieve the above purpose, the present utility model provides the following technical solutions: a kit for western blot detection comprising: a bottom plate with an opening at the top;

the groove is arranged in the opening and positioned at the top of the bottom plate and is used for placing the detection film;

the sealing mechanism comprises cover plates which are arranged above the grooves and can be pulled out in the openings, sealing strips which are symmetrically arranged at two symmetrical ends of the bottom plate, and two sealing strips are respectively filled in gaps between the cover plates at two sides and the bottom plate.

Preferably, the device further comprises a collection mechanism for collecting the antibody solution in the groove, the collection mechanism comprising:

offer the storage tank on the bottom plate side, install the catheter in the storage tank, the one end of catheter extends to in the recess, just the one end of catheter is in a corner of recess inside, the other end of catheter just is in the storage tank and has screwed the stifled cap.

Preferably, a clamping seat is fixed on the inner wall of the storage groove, the liquid guide pipe is clamped on the clamping seat, a buckling groove is formed in the side face of the bottom plate, and one end of the buckling groove extends into the storage groove.

Preferably, the outside of sealing strip is fixed with the curb plate, the inboard of curb plate one end is fixed with the elastic rod, the flexible hole has been seted up to one side on the bottom plate and close to the elastic rod, slidable mounting has the slider of being connected with elastic rod one end in the flexible hole.

Preferably, the inner walls of the two symmetrical sides of the opening are provided with sliding grooves, and the cover plate is slidably arranged in the sliding grooves on the two sides.

Preferably, the two symmetrical sides of the top of the cover plate are both fixed with a shifting block, and the shifting block is of a plate-shaped structure.

Compared with the prior art, the utility model has the following beneficial effects:

(1) According to the utility model, the detection film can be embedded on the bottom plate through the grooves, a small amount of antibody solution can fully cover the detection film during detection, meanwhile, external dust is prevented from entering through the cover plate, the cover plate and the bottom plate are sealed under the action of the sealing strip, liquid volatilization is avoided, and the problems that the whole film cannot be covered by a small amount of liquid, liquid volatilization is possibly caused by unsealing during long-time incubation, and the film is dried and polluted are solved.

(2) According to the utility model, the antibody solution in the groove can be collected through the collecting mechanism, one end of the device with the liquid guide tube is vertically placed, the liquid guide tube is taken out of the containing groove, the blocking cap is screwed off, and the antibody solution in the groove is discharged outwards through the liquid guide tube, so that the collection of the antibody solution is completed, and the device is convenient for later repeated use.

Drawings

FIG. 1 is an expanded schematic view of the present utility model;

FIG. 2 is a schematic view of the fit of the elastic rod and the telescopic hole of the present utility model;

FIG. 3 is a top view of the present utility model;

FIG. 4 is a schematic view showing the fit of a catheter and a groove according to the present utility model;

FIG. 5 is a side view of the present utility model;

FIG. 6 is a side view of FIG. 1;

in the figure: 1. a side plate; 2. a shifting block; 3. a cover plate; 4. an opening; 5. a bottom plate; 6. a groove; 7. an elastic rod; 8. a sealing strip; 9. a slide block; 10. a telescopic hole; 11. a chute; 12. a catheter; 13. a storage groove; 14. a buckling groove; 15. a blocking cap; 16. a clamping seat.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-4 and fig. 6, the present utility model provides the following technical solutions: a kit for western blot detection comprising: a bottom plate 5 with an opening 4 at the top;

a groove 6 which is arranged in the opening 4 and is positioned at the top of the bottom plate 5 and is used for placing a detection film;

the sealing mechanism for sealing the detection film comprises a cover plate 3 which is arranged above a groove 6 and can be pulled in an opening 4, and sealing strips 8 which are symmetrically arranged at two symmetrical ends of a bottom plate 5, wherein the two sealing strips 8 are respectively filled in gaps between the cover plates 3 and the bottom plate 5 at two sides.

Through the technical scheme:

the cover plate 3 is pulled out to one side of the bottom plate 5 in the opening 4, the groove 6 in the opening 4 is exposed at the moment, the detection film is placed in the groove 6, a small amount of antibody solution is added, the cover plate 3 is pushed reversely, the cover plate 3 is reset, external dust is prevented from falling into the groove 6, meanwhile, the sealing strips 8 on two sides are filled in gaps between the cover plate 3 and the bottom plate 5 on two sides, and volatilization of liquid in the groove 6 is avoided.

Further, to the sealing strip 8 that sets up above, the outside of sealing strip 8 is fixed with curb plate 1, the inboard of curb plate 1 one end is fixed with elastic rod 7, the shrinkage cavity 10 has been seted up to one side on the bottom plate 5 and close to elastic rod 7, slidable mounting has the slider 9 of being connected with elastic rod 7 one end in the shrinkage cavity 10, be convenient for push into the gap between apron 3 and the bottom plate 5 with sealing strip 8 through curb plate 1, accomodate curb plate 1 and sealing strip 8 in one side of bottom plate 5 through elastic rod 7 and slider 9, avoid sealing strip 8 to lose, when sealing strip 8 fills in the gap, elastic rod 7 drives slider 9 and removes in the shrinkage cavity 10, thereby accomodate elastic rod 7 in the shrinkage cavity 10, elastic rod 7 is made for the rubber material.

Further, for the cover plate 3, the inner walls of the two symmetrical sides of the opening 4 are provided with the sliding grooves 11, the cover plate 3 is slidably arranged in the sliding grooves 11 on the two sides, and the cover plate 3 can be pulled back and forth in the opening 4 through the sliding grooves 11, so that the device can be opened conveniently.

Further, to the above-mentioned apron 3 that sets up, the both sides of the top symmetry of apron 3 all are fixed with the plectrum 2, and the plectrum 2 is platelike structure, is convenient for promote apron 3 through the plectrum 2.

Referring to fig. 4-5, the device further comprises a collection mechanism for collecting the antibody solution in the groove 6, the collection mechanism comprises:

a receiving groove 13 is formed in the side face of the bottom plate 5, a liquid guide tube 12 is arranged in the receiving groove 13, one end of the liquid guide tube 12 extends into the groove 6, one end of the liquid guide tube 12 is positioned at one corner of the inside of the groove 6, and a blocking cap 15 is screwed in the receiving groove 13 at the other end of the liquid guide tube 12.

Through the technical scheme:

when the antibody solution is required to be collected, the bottom plate 5 is erected, one end with the liquid guide tube 12 is arranged at the lowest position, the liquid guide tube 12 is taken out of the containing groove 13, the blocking cap 15 is screwed down, the liquid in the groove 6 is discharged outwards through the liquid guide tube 12, the collection is facilitated, the blocking cap 15 is screwed on the liquid guide tube 12 after the collection, and the liquid guide tube 12 is contained in the containing groove 13, so that the later use is facilitated.

Further, for the catheter 12 arranged as described above, the inner wall of the accommodating groove 13 is fixed with the clamping seat 16, the catheter 12 is clamped on the clamping seat 16, the side surface of the bottom plate 5 is provided with the buckling groove 14, one end of the buckling groove 14 extends into the accommodating groove 13, the catheter 12 is conveniently limited in the accommodating groove 13 through the clamping seat 16, the catheter 12 is separated from the clamping seat 16 when in use, at this time, the catheter 12 automatically pops out of the accommodating groove 13, the finger is conveniently inserted into the accommodating groove 13 through the buckling groove 14, and the catheter 12 is conveniently taken out of the accommodating groove 13.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A kit for detecting a protein by blotting, comprising: a bottom plate (5) with an opening (4) at the top;

the groove (6) is arranged in the opening (4) and is positioned at the top of the bottom plate (5) and is used for placing the detection film;

sealing mechanism for detecting the membrane and sealing, sealing mechanism is including installing apron (3) in recess (6) top and can pull in opening (4) to and install sealing strip (8) at bottom plate (5) symmetry both ends symmetrically, two sealing strip (8) are filled respectively in the gap between apron (3) and bottom plate (5) of both sides.

2. The kit for detecting protein blotting according to claim 1, wherein: also included is a collection mechanism for collecting the antibody solution in the recess (6), the collection mechanism comprising:

offer storage tank (13) on bottom plate (5) side, install catheter (12) in storage tank (13), in the one end of catheter (12) extends to recess (6), just the one end of catheter (12) is in a corner of recess (6) inside, the other end of catheter (12) just is in storage tank (13) and has screwed stifled cap (15).

3. The kit for detecting protein blotting according to claim 2, wherein: the liquid guide tube is characterized in that a clamping seat (16) is fixed on the inner wall of the storage groove (13), the liquid guide tube (12) is clamped on the clamping seat (16), a buckling groove (14) is formed in the side face of the bottom plate (5), and one end of the buckling groove (14) extends into the storage groove (13).

4. The kit for detecting protein blotting according to claim 1, wherein: the outside of sealing strip (8) is fixed with curb plate (1), the inboard of curb plate (1) one end is fixed with elastic rod (7), telescopic hole (10) have been seted up on bottom plate (5) and one side that is close to elastic rod (7), slidable mounting has slider (9) that are connected with elastic rod (7) one end in telescopic hole (10).

5. The kit for detecting protein blotting according to claim 1, wherein: the inner walls of the two symmetrical sides of the opening (4) are respectively provided with a chute (11), and the cover plate (3) is slidably arranged in the chute (11) on the two sides.

6. The kit for detecting protein blotting according to claim 1, wherein: the two symmetrical sides of the top of the cover plate (3) are respectively fixed with a shifting block (2), and the shifting blocks (2) are of plate-shaped structures.