CN218989285U

CN218989285U - Cancer cell culture reaction dish

Info

Publication number: CN218989285U
Application number: CN202223390273.0U
Authority: CN
Inventors: 夏献民; 李夏; 孙项
Original assignee: Wuhan Yi Bio Technology Co ltd
Current assignee: Wuhan Yi Bio Technology Co ltd
Priority date: 2022-12-17
Filing date: 2022-12-17
Publication date: 2023-05-09
Anticipated expiration: 2032-12-17

Abstract

The utility model discloses a cancer cell culture reaction vessel which comprises a reaction vessel body, wherein a separation ring is arranged in the reaction vessel body, two vertical pipes are inserted into the top of the separation ring, a second reaction layer is fixedly connected between the two vertical pipes, a top cover is arranged at the top of the reaction vessel body, and the top ends of the two vertical pipes penetrate through and are connected to the top of the top cover in a sliding manner. The inner surface of the reaction vessel body is divided into two areas which are not communicated through the separating ring in the reaction vessel body, so that different cultivation experiment comparison can be conveniently carried out on cell culture solutions in different areas, after the top cover is opened, two vertical pipes are pulled up to bring out the second reaction layer, then the inner surface of the reaction vessel body at the bottom is subjected to cell cultivation, then the two vertical pipes are reinserted on the separating ring, and then cancer cell cultivation is carried out on the second reaction layer, so that the space utilization rate of the reaction vessel is effectively increased.

Description

Cancer cell culture reaction dish

Technical Field

The utility model relates to the technical field of culture dishes, in particular to a cancer cell culture reaction dish.

Background

In recent years, research has found that there exists a group of cells with normal stem cell characteristics in tumor tissues, and the cells have self-specific expression molecules, and the group of cells is named as tumor stem cells, and the tumor stem cells are the sources of tumor recurrence and metastasis, and the research and cultivation of a cancer cell culture reaction vessel requires the use of a cancer cell culture reaction vessel, and the following defects also exist in the current reaction vessel:

1. the existing cancer cell culture reaction vessel is simple in structure, and because tumor cancer cells generally grow in an attached mode, the tumor cancer cells are limited by the inner surface area of the culture reaction vessel, and the upper limit of culture of a single reaction vessel is low;

2. the existing culture reaction vessel cannot compare the culture efficiency of nutrient solutions with different proportions in the same reaction vessel, and needs to occupy more equipment.

Disclosure of Invention

Aiming at the problems in the prior art, the utility model aims to provide a cancer cell culture reaction vessel which overcomes the defects in the background art.

In order to solve the problems, the utility model adopts the following technical scheme.

The utility model provides a cancer cell culture reaction dish, includes the reaction dish main part, the inside of reaction dish main part is provided with the spacer ring, peg graft at the top of spacer ring has two risers, two fixedly connected with second reaction layer between the riser, the top cap has been seted up at the top of reaction dish main part, two the top of riser all runs through and sliding connection to the top of top cap.

As a further description of the above technical solution: smooth protrusions are arranged on the inner surfaces of the second reaction layer and the reaction vessel main body.

As a further description of the above technical solution: two transverse holes are formed in the separation ring and are respectively communicated with the two vertical pipes.

As a further description of the above technical solution: the inside of two standpipe all fixedly connected with first filter screen.

As a further description of the above technical solution: two vent holes are formed in the top cover, and a second filter screen is fixedly connected to the inside of each vent hole.

Compared with the prior art, the utility model has the advantages that:

this scheme is through nimble structure combination, increases unit and cultivates area and cultivates the upper limit to the convenient cultivation efficiency contrast to different ratio nutrient solution reduces the advantage of equipment use quantity.

Drawings

FIG. 1 is a schematic cross-sectional elevation view of the present utility model;

FIG. 2 is a schematic view of the portion A in FIG. 1;

fig. 3 is a schematic diagram of the B part structure in fig. 1.

The reference numerals in the figures illustrate:

1. a reaction vessel body; 2. a spacer ring; 21. a transverse hole; 3. a standpipe; 31. a first filter screen; 4. a second reaction layer; 5. a top cover; 51. a vent hole; 52. a second filter screen; 6. a protrusion.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model;

referring to fig. 1-3, the cancer cell culture reaction vessel of the present utility model comprises a reaction vessel body 1, wherein a separation ring 2 is disposed in the reaction vessel body 1, two vertical pipes 3 are inserted into the top of the separation ring 2, a second reaction layer 4 is fixedly connected between the two vertical pipes 3, a top cover 5 is disposed at the top of the reaction vessel body 1, and the top ends of the two vertical pipes 3 penetrate through and are slidably connected to the top of the top cover 5.

According to the utility model, the inner surface of the reaction vessel body 1 is divided into two areas which are not communicated through the separating ring 2, so that different cultivation experiment comparison is conveniently carried out on cell culture solutions in different areas, after the top cover 5 is opened, two vertical pipes 3 are pulled out upwards to bring out the second reaction layer 4, then the inner surface of the reaction vessel body 1 at the bottom is subjected to cell cultivation, then the two vertical pipes 3 are reinserted on the separating ring 2, and then cancer cells are cultivated on the second reaction layer 4, so that the space utilization rate of the reaction vessel is effectively increased, the advantages of flexible structure combination of the device, unit cultivation area and upper cultivation limit increase, convenience in comparison of cultivation efficiency of nutrient solutions in different proportions and reduction of the use quantity of equipment are realized;

please refer to fig. 1, wherein: the second reaction layer 4 and the inner surface of the reaction vessel body 1 are both provided with smooth bulges 6.

In the present utility model, the rounded protrusions 6 increase the inner surface area of the device and increase the cell culture amount per unit space.

Please refer to fig. 2, wherein: the separation ring 2 is provided with two transverse holes 21, and the two transverse holes 21 are respectively communicated with the two vertical pipes 3.

In the utility model, the two transverse holes 21 are respectively communicated with the two vertical pipes 3, so that the inside of the separating ring 2 is communicated with the outside air.

Please refer to fig. 3, wherein: the inside of both standpipe 3 is fixedly connected with first filter screen 31.

In the present utility model, air is filtered through the first filter 31.

Please refer to fig. 1, wherein: two vent holes 51 are formed in the top cover 5, and a second filter screen 52 is fixedly connected to the inner parts of the two vent holes 51.

In the utility model, the inside of the reaction vessel body 1 is communicated with the outside through the vent hole 51, and the second filter screen 52 filters air to ensure the cleanness of the inside.

The above description is only of the preferred embodiments of the present utility model; the scope of the utility model is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present utility model, and the technical solution and the improvement thereof are all covered by the protection scope of the present utility model.

Claims

1. A cancer cell culture reaction vessel, comprising a reaction vessel main body (1), characterized in that: the inside of reaction dish main part (1) is provided with separating ring (2), peg graft at the top of separating ring (2) has two standpipe (3), two fixedly connected with second reaction layer (4) between standpipe (3), top cap (5) have been seted up at the top of reaction dish main part (1), two the top of standpipe (3) all runs through and sliding connection to the top of top cap (5).

2. A cancer cell culture reaction vessel according to claim 1, wherein: smooth bulges (6) are arranged on the inner surfaces of the second reaction layer (4) and the reaction vessel main body (1).

3. A cancer cell culture reaction vessel according to claim 1, wherein: two transverse holes (21) are formed in the separation ring (2), and the two transverse holes (21) are respectively communicated with the two vertical pipes (3).

4. A cancer cell culture reaction vessel according to claim 1, wherein: the two vertical pipes (3) are fixedly connected with first filter screens (31) inside.

5. A cancer cell culture reaction vessel according to claim 1, wherein: two vent holes (51) are formed in the top cover (5), and second filter screens (52) are fixedly connected to the inner parts of the two vent holes (51).