CN218921306U

CN218921306U - Zebra fish respiration detection device

Info

Publication number: CN218921306U
Application number: CN202222791812.5U
Authority: CN
Inventors: 贾良权; 高璐; 臧影
Original assignee: Huzhou University
Current assignee: Huzhou University
Priority date: 2022-10-21
Filing date: 2022-10-21
Publication date: 2023-04-28
Anticipated expiration: 2032-10-21

Abstract

The utility model discloses a zebra fish respiration detection device, and relates to the technical field of zebra fish research. The device comprises an operation pool, wherein a water tank is arranged on one side of the operation pool, the water tank is connected with the operation pool through a through pipe, a valve is arranged on the through pipe, and an oxygen meter and an acid-base detector are arranged on one side of the operation pool. According to the utility model, through the arrangement of the sucker, the oxygen meter, the acid-base detector, the water tank and the like, in the process of performing the heart injection of the zebra fish, the device can judge whether the water quality is in a proper demand state of the zebra fish through the oxygen meter and the acid-base detector, and when the oxygen and the pH value in water are both over or under the living demand of the zebra fish, the valve is manually opened to replace the water quality, so that the zebra fish can be in a normal living environment, can breathe normally, and is maintained and researched and tested.

Description

Zebra fish respiration detection device

Technical Field

The utility model relates to the technical field of zebra fish research, in particular to a zebra fish respiration detection device.

Background

Zebra fish and human genes have a high degree of similarity of 87%, and are prominent as model organisms, which means that the experimental results are suitable for the human body in most cases. The method can be used for monitoring water quality environment, and cell marking technology, tissue transplanting technology, mutation technology, haploid breeding technology, transgenic technology, gene activity inhibition technology and the like of zebra fish are mature, and thousands of zebra fish embryo mutants are excellent resources for researching embryo development molecular mechanisms, and can be used as human disease models, so that zebra fish has become one of the most important vertebrate development biological modes.

In view of the excellent research value of zebra fish, a great deal of research courses are generated for zebra fish, application number 201710486466.0 is a visual servo zebra fish heart injection method, which is mentioned in the background art as "since in a specific research, medicines need to be injected into the heart of the zebra fish (for example, after fluorescent reagent is injected into the heart of young zebra fish, the flowing and distribution situation of the fluorescent reagent along with blood is observed), the change of the zebra fish is observed, but the conventional method is to manually inject the zebra fish heart, the success rate is low, then the research needs to ensure that the zebra fish is in a living and relatively healthy state in the implementation process, and in this process, the zebra fish needs to be fixed, the heart position of the zebra fish needs to be determined, and the injection process is not a short process, so that how to observe and maintain the living state of the zebra fish is a problem which needs to be solved in a plurality of steps, based on this, we propose a zebra fish respiration detection device to solve the problem.

Disclosure of Invention

The utility model aims to provide a zebra fish respiration detection device for solving the problems in the background technology.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a zebra fish breathes detection device, includes the operation pond, operation pond one side is provided with the water tank, is connected through the siphunculus between water tank and the operation pond, is provided with the valve on the siphunculus, and operation pond one side is provided with oxygen meter and acid-base detector, judges whether quality of water is in the suitable demand state of zebra fish through oxygen meter and acid-base detector, in aquatic oxygen and pH value all surpassed or be less than the living demand of zebra fish, the manual valve of opening carries out quality of water replacement, ensures that zebra fish can be in normal living environment, can breathe normally.

Preferably, the height of the inner wall at the bottom of the water tank is higher than that of the inner wall at the bottom of the operation tank, and the through pipe is arranged at a position close to the top of the operation tank.

Preferably, a water draining groove is formed in one end of the operation pool, and the water draining groove is arranged at a position close to the top of the operation pool.

Preferably, the width between the inner walls at two sides of the operation pool is not smaller than cm, the length between the inner walls at two ends of the operation pool is not smaller than cm, the width between the water tank and the operation pool is not smaller than cm, and the valve is a knob valve.

Preferably, the bottom of the operation pool is fixedly connected with an air pipe communicated with the inside of the operation pool, and the tail end of the air pipe is fixedly connected with a sucker arranged in the operation pool.

Compared with the prior art, the utility model has the beneficial effects that:

this zebra fish breath detection device passes through the setting of sucking disc, oxygen meter, acid-base detector and water tank etc. the device is carrying out zebra fish heart injection in-process, can judge through oxygen meter and acid-base detector that quality of water is in the suitable demand state of zebra fish, in aquatic oxygen and pH value all surpassed or be less than the living demand of zebra fish, the manual valve of opening carries out quality of water replacement, ensures that the zebra fish can be in normal living environment in, can breathe normally, maintains the going on of research experiment.

This zebra fish breath detection device simple structure can not interfere the implementation step of experimenter in the research experiment in-process, and uses very conveniently, when improving zebra fish survival rate, can improve the advancing process of research experiment, and the practicality is stronger.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present utility model;

FIG. 2 is a left side schematic view of the present utility model;

FIG. 3 is a schematic diagram of the top structure of the operation pool of the present utility model.

In the figure: 1. an operation pool; 2. a chassis; 3. a water tank; 4. a through pipe; 5. a valve; 6. an air pipe; 7. a suction cup; 8. an oxygen meter; 9. an acid-base detector; 10. a water drain tank.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that, in the description of the present utility model, the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, only for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

Furthermore, it should be understood that the dimensions of the various elements shown in the figures are not drawn to actual scale, e.g., the thickness or width of some layers may be exaggerated relative to other layers for ease of description.

It should be noted that like reference numerals and letters refer to like items in the following figures, and thus once an item is defined or illustrated in one figure, no further detailed discussion or description thereof will be necessary in the following description of the figures.

As shown in fig. 1 to 3, the present utility model provides a technical solution: the zebra fish respiration detection device comprises an operation pool 1, wherein a bottom frame 2 is fixed at the bottom of the operation pool 1, the bottom frame 2 is fixed on the top plane of a test bed, the height of the bottom frame 2 can be set according to conditions, the operation is convenient, a water tank 3 is arranged on one side of the operation pool 1, the water tank 3 is connected with the operation pool 1 through a through pipe 4, and a valve 5 is arranged on the through pipe 4;

the operation pond 1 one side is provided with oxygen meter 8 and acid-base detector 9, and the device is in carrying out zebra fish heart injection in-process, can judge whether quality of water is in the suitable demand state of zebra fish through oxygen meter 8 and acid-base detector 9, and in aquatic oxygen and pH value all exceeded or were less than the living demand of zebra fish, manual valve 5 was opened and quality of water replacement was carried out, ensures that the zebra fish can be in normal living environment, can breathe normally.

As shown in fig. 1 to 3, to ensure the smooth implementation of this embodiment, it should be understood that the height of the inner wall of the bottom of the water tank 3 is higher than that of the inner wall of the bottom of the operation tank 1, the through pipe 4 is disposed near the top of the operation tank 1, and after the valve 5 is opened, the water in the water tank 3 can flow into the operation tank 1 under the action of gravity, so as to ensure the experimental progress, the water in the water tank 3 is at least three times the volume of the operation tank 1.

As shown in fig. 1 to 3, further, a water draining groove 10 is formed at one end of the operation tank 1, the water draining groove 10 is arranged at a position close to the top of the operation tank 1, and water in the operation tank 1 can be drained from the water draining groove 10 in an overflow state, so that internal water can be drained and rapid flowing of water can be avoided.

As shown in fig. 1 to 3, to ensure smooth implementation of this embodiment, it is required to know that the width between the inner walls at two sides of the operation tank 1 is not less than 10 cm, the length between the inner walls at two ends of the operation tank 1 is not less than 15 cm, the width between the water tank 3 and the operation tank 1 is not less than 30 cm, the valve 5 is a knob valve, the design of the operation tank 1 can enable an experimenter to normally act in the operation tank 1, and the research progress of the experimenter is not hindered by the water tank 3 at one side, and the knob valve is arranged to facilitate the opening and closing of the through pipe 4, so that the operation is simpler.

As shown in fig. 1 to 3, further, the bottom of the operation pool 1 is fixedly connected with an air pipe 6 communicated with the inside of the operation pool 1, the tail end of the air pipe 6 is fixedly connected with a sucker 7 arranged in the operation pool 1, before the research experiment starts, zebra fish is attached to the sucker 7, the diameter of the sucker 7 is not more than three centimeters, air is pumped through the air pipe 6, the zebra fish is attached to the sucker 7, water is injected into the operation pool 1, and the zebra fish can be effectively limited.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a zebra fish breath detection device, includes operation pond (1), its characterized in that: a water tank (3) is arranged at one side of the operation pool (1), the water tank (3) is connected with the operation pool (1) through a through pipe (4), and a valve (5) is arranged on the through pipe (4);

an oxygen meter (8) and an acid-base detector (9) are arranged on one side of the operation pool (1).

2. The zebra fish breath detection device of claim 1, wherein: the height of the inner wall at the bottom of the water tank (3) is higher than that of the inner wall at the bottom of the operation pool (1), and the through pipe (4) is arranged at the position close to the top of the operation pool (1).

3. The zebra fish breath detection device of claim 1, wherein: one end of the operation pool (1) is provided with a water draining groove (10), and the water draining groove (10) is arranged at a position close to the top of the operation pool (1).

4. The zebra fish breath detection device of claim 1, wherein: the width between the inner walls of the two sides of the operation pool (1) is not less than 10 cm, the length between the inner walls of the two ends of the operation pool (1) is not less than 15 cm, the width between the water tank (3) and the operation pool (1) is not less than 30 cm, and the valve (5) is a knob valve.

5. The zebra fish breath detection device of claim 1, wherein: the bottom of the operation pool (1) is fixedly connected with an air pipe (6) communicated with the inside of the operation pool (1), and the tail end of the air pipe (6) is fixedly connected with a sucker (7) arranged in the operation pool (1).