CN218842165U

CN218842165U - Real-time fluorescence PCR detector

Info

Publication number: CN218842165U
Application number: CN202222573107.8U
Authority: CN
Inventors: 吉俊利; 周志图
Original assignee: Bioteke Corp
Current assignee: Bioteke Corp
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2023-04-11
Anticipated expiration: 2032-09-28

Abstract

The utility model discloses a real-time fluorescence PCR detector, including hollow shell, the lateral wall of shell sets up a plurality of thermovent, the fixed mount that sets up in the cavity of shell, slide on the mount and set up the loading board that is used for bearing the alternating temperature module, the shell sets up the exit corresponding with the loading board, the loading board sets up the closing plate that is used for sealing the exit with the side that the exit corresponds is fixed, set up a plurality of on the loading board and be used for dismantling the bearing mouth of placing the alternating temperature module, the bottom of loading board is fixed and is set up a plurality of fin type radiator; the top of the temperature changing module is provided with a plurality of placing openings for placing the biological chips, and the top of the fixing frame is provided with a camera and an output end of the light source, which correspond to the position of the bearing opening. The utility model aims at providing a can short-term test PCR reaction obtain real-time fluorescence PCR detector of result.

Description

Real-time fluorescence PCR detector

Technical Field

The utility model relates to a PC reagent detects, especially relates to a real-time fluorescence PCR detector.

Background

PCR (polymerase chain reaction) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased. Therefore, PCR technology is often used to detect minute amounts of bacteria or viruses.

The general procedure for PCR was: firstly, DNA is denatured at high temperature (about 95 ℃) and becomes single-stranded, then when the temperature is reduced to low temperature (about 60 ℃), primers and the single strands are combined according to the principle of base complementary pairing, finally, the temperature is adjusted to the optimal reaction temperature (about 72 ℃), and DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose (5 '-3').

In the PCR reaction process, in order to facilitate Real-time monitoring and analysis, a fluorophore needs to be added into the PCR reaction system, namely a Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) method. The current mainstream real-time fluorescent quantitative PCR method mainly comprises a dye method and a probe method.

The existing real-time fluorescent PCR detector has the following problems no matter the dye method and the probe method are adopted: 1. the structure is complex, the purchase cost and the installation and debugging cost are high, and the movement is inconvenient; 2. the single-hole sample size is large, the single-hole sample size is limited by a heat dissipation structure of the detector, the heat dissipation efficiency is low, the temperature reduction is slow, and the PCR reaction time is long.

Disclosure of Invention

Utility model purpose: the utility model aims at providing a can short-term test PCR reaction obtain real-time fluorescence PCR detector of result.

The technical scheme is as follows: the utility model discloses a real-time fluorescence PCR detector, including hollow shell, the lateral wall of shell sets up a plurality of thermovent, the fixed mount that sets up in the cavity of shell, slide on the mount and set up the loading board that is used for bearing the alternating temperature module, the shell sets up the import and export corresponding with the loading board, the loading board sets up the closure plate that is used for sealing the import and export with the side that the import and export corresponds is fixed, set up a plurality of bearing port that is used for can dismantle the alternating temperature module of placing on the loading board, the bottom of loading board is fixed and is set up a plurality of fin formula radiator; the temperature changing module is detachably arranged in the bearing port, a plurality of placing ports used for placing the biochips are arranged at the top of the temperature changing module, and the top of the fixing frame is provided with a camera and an output end of the light source, wherein the camera and the output end correspond to the bearing port. The biochip is used for bearing detection objects and detection reagents, a plurality of micropores are formed in the biochip, the biochip is made of special optical plastics, and the biochip is good in light transmittance and does not react with detected samples and the detection reagents.

Furthermore, the side surface of the finned radiator is fixedly provided with a fan corresponding to the position of the finned radiator.

Further, a rack is fixedly arranged on the side face of the bearing plate, a sliding motor corresponding to the rack is fixedly arranged on the side face of the fixing frame, and a gear meshed with the rack is fixedly arranged at the output end of the sliding motor.

Furthermore, the temperature changing module comprises a bottom plate made of metal, a hollow temperature changing frame body is fixedly arranged on the top surface of the bottom plate, a plurality of semiconductor refrigerators are fixedly arranged in the hollow part of the temperature changing frame body, a heating plate is fixedly arranged on the top surface of each semiconductor refrigerator, a placing plate is fixedly arranged on the top surface of each heating plate, and a plurality of placing openings used for placing biochips are formed in the top surface of each placing plate. The semiconductor refrigerator is a device which is made of semiconductor materials and realizes heat transfer from top to bottom (when heat dissipation is needed) or from bottom to top (when heating is needed) in a PN junction mode.

Furthermore, a locking device for locking with the side wall of the bearing opening is arranged on the side surface of the temperature changing frame body.

Furthermore, the side wall of the bottom plate is connected with the finned radiator through a plurality of radiating pipes.

Furthermore, a hot cover device is arranged above the bearing plate in a sliding mode, and a hot cover is arranged at the bottom of the hot cover device. The thermal cover is used for preventing liquid in the detection object from evaporating in the thermal cycle of detection and forming condensed water on the biochip, so that the detection object is concentrated and the detection result is inaccurate.

Furthermore, the hot cover device comprises a connecting plate, the top surface of the connecting plate is fixedly connected with the output ends of the plurality of longitudinal motors, the connecting plate and the two corresponding sides of the fixing frame are provided with sliding blocks, the fixing frame is provided with sliding rails connected with the sliding blocks in a sliding manner, the bottom of the connecting plate is provided with a plurality of sliding rods in a sliding manner, the bottom of each sliding rod is fixedly provided with a hot cover, a spring is sleeved outside each sliding rod, and the two ends of each spring respectively abut against the hot cover and the connecting plate.

Furthermore, a hollow light shield is fixedly arranged at the top of the fixing frame, a detection port corresponding to the position of the biochip is formed in the bottom of the light shield, a filter wheel is rotatably arranged in a cavity of the light shield, and a plurality of optical filters are arranged on the filter wheel. The PCR detection needs to use light rays with various wavelengths, the light rays with different wavelengths need to correspond to different optical filters, and the filter wheel switches different optical filters through rotation.

Furthermore, the top surface of the filter wheel is fixedly connected with a rotating motor.

Furthermore, a laser generator is fixedly arranged in the cavity of the shell, a surface light source module is arranged on the side face of the light shield, the laser generator is connected with the surface light source module through optical fibers, and a plurality of refractors corresponding to the surface light source module are arranged on the bottom face of the filter wheel. The surface light source module is used for homogenizing laser.

Has the beneficial effects that: compared with the prior art, the utility model, have following advantage: compared with the prior art, the utility model, have following advantage: the utility model discloses set up finned radiator in the bottom of alternating temperature module, set up the fan in finned radiator's side, when PCR reaction need be cooled down, possible rapid cooling shortens the time of PCR reaction, shortens the time that obtains the result, the utility model discloses built-in hot lid device can accomplish as far as possible that the result is accurate, reduces the number of times of reinspection.

Drawings

Fig. 1 is a perspective view of the present invention.

Fig. 2 is a perspective view of the present invention with an angle of the housing removed.

Fig. 3 is a perspective view of the present invention with another angle of the housing removed.

Fig. 4 is a perspective view of the medium temperature changing module of the present invention.

Fig. 5 is an exploded view of the structure of the medium temperature changing module of the present invention.

Fig. 6 is a schematic view of the combination of the medium temperature varying module, the finned radiator and the fan according to the present invention.

Fig. 7 is a schematic structural view of the middle heat cover device of the present invention.

FIG. 8 is a sectional view of the mask, filter wheel, rotary motor and area light source module.

Wherein: 1. a housing; 101. a heat dissipation port; 102. a closing plate; 2. a light shield; 3. a fixed mount; 301. a slide rail; 4. a slide motor; 5. a fan; 6. a finned heat sink; 7. a carrier plate; 701. a rack; 8. a temperature changing module; 801. placing the plate; 8011. a placement port; 802. heating plates; 803. a semiconductor refrigerator; 804. a temperature changing frame body; 805. a base plate; 806. a locking device; 9. a thermal cover device; 901. a longitudinal motor; 902. a connecting plate; 903. a hot lid; 904. a slide bar; 905. a slider; 10. a surface light source module; 11. a camera; 12. rotating the motor; 13. a radiating pipe; 14. a light filtering wheel; 15. a fixed block; 16. a laser generator; 1601. an optical fiber; 17. a power source.

Detailed Description

The technical solution of the present invention will be further explained with reference to the accompanying drawings.

Referring to fig. 1 to 8, the present invention discloses a real-time fluorescence PCR detector, which includes a hollow housing 1, a plurality of heat dissipation openings 101 are disposed on a side wall of the housing 1, a fixing frame 3 is fixedly disposed in a cavity of the housing 1, a rack 701 is fixedly disposed on a side surface of a bearing plate 7, a sliding motor 4 corresponding to the rack 701 is fixedly disposed on a side surface of the fixing frame 3, a gear meshed with the rack 701 is fixedly disposed on an output end of the sliding motor 4, the housing 1 is provided with an inlet and an outlet corresponding to the bearing plate 7, a sealing plate 102 for sealing the inlet and the outlet is fixedly disposed on a side surface of the bearing plate 7 corresponding to the inlet and outlet, a plurality of bearing openings for detachably placing a temperature varying module 8 are disposed on the bearing plate 7, two fin type heat sinks 6 are fixedly disposed on a bottom of the bearing plate 7, and a fan 5 corresponding to a position of a side surface of each fin type heat sink 6 is fixedly disposed; the temperature changing module 8 is detachably arranged in the bearing port, the temperature changing module 8 comprises a bottom plate 805 made of metal, a hollow temperature changing frame body 804 is fixedly arranged on the top surface of the bottom plate 805, a plurality of semiconductor refrigerators 803 are fixedly arranged in the hollow part of the temperature changing frame body 804, a heating plate 802 is fixedly arranged on the top surface of each semiconductor refrigerator 803, a placing plate 801 is fixedly arranged on the top surface of each heating plate 802, a plurality of placing ports 8011 for placing biochips are formed in the top surface of each placing plate 801, locking devices 806 for locking with the side walls of the bearing port are arranged on the side surfaces of the temperature changing frame body 804, and the side walls of the bottom plate 805 are connected with the fin-type heat radiator 6 through a plurality of heat radiating pipes 13; a hot cover device 9 is slidably arranged above the bearing plate 7, the hot cover device 9 comprises a connecting plate 902, top surfaces of two sides of the connecting plate 902 are fixedly connected with output ends of two longitudinal motors 901, the two longitudinal motors 901 can respectively control the positions of the connecting plate 902 to better control the posture of the connecting plate 902, sliding blocks 905 are arranged on two sides of the connecting plate 902 corresponding to the fixed frame 3, a sliding rail 301 slidably connected with the sliding blocks 905 is arranged on the fixed frame 3, a plurality of sliding rods 904 are slidably arranged at the bottom of the connecting plate 902, a hot cover 903 is fixedly arranged at the bottom of the sliding rods 904, springs are sleeved outside the sliding rods 904, and two ends of the springs are respectively abutted against the hot cover 903 and the connecting plate 902; fixed hollow lens hood 2 that sets up in mount 3's top, the detection mouth corresponding with the biochip position is seted up to the bottom of lens hood 2, the cavity internal rotation of lens hood 2 sets up filter wheel 14, filter wheel 14's top surface and rotation motor 12 fixed connection, set up a plurality of filtering breach on the filter wheel 14, the filtering breach is used for installing the light filter (not drawn), the cavity internal fixation of shell 1 sets up laser generator 16, the side of lens hood 2 sets up surface light source module 10, laser generator 16 is connected with surface light source module 10 through optic fibre 1601, the bottom surface of filter wheel 14 sets up a plurality of and the corresponding refractor of surface light source module 10 (not drawn), fixed setting and the corresponding camera 11 of bearing the mouth position are passed through at the top of lens hood 2 through fixed block 15. The top of the rear side of the fixed frame 3 is fixedly provided with a power supply 17,

the biochip is used for bearing detection objects and detection reagents, a plurality of micropores are formed in the biochip, the biochip is made of special optical plastics, and the biochip is good in light transmittance and does not react with detected samples and the detection reagents. The thermal cap 903 is used for preventing the evaporation of the liquid inside the test object and the formation of condensed water on the biochip during the thermal cycle of the test, thereby concentrating the test object and making the test result inaccurate. The PCR detection needs to use light rays with various wavelengths, the light rays with different wavelengths need to correspond to different optical filters, and the filter wheel 14 switches the different optical filters by rotating. The surface light source module 10 is used for homogenizing laser light.

Claims

1. A real-time fluorescence PCR detector is characterized in that: the heat dissipation device comprises a hollow shell (1), wherein a plurality of heat dissipation ports (101) are formed in the side wall of the shell (1), a fixing frame (3) is fixedly arranged in a cavity of the shell (1), a bearing plate (7) is arranged on the fixing frame (3) in a sliding mode, an inlet and an outlet corresponding to the bearing plate (7) are formed in the shell (1), a sealing plate (102) is fixedly arranged on the side face, corresponding to the inlet and the outlet, of the bearing plate (7), a bearing port is formed in the bearing plate (7), and a plurality of fin-type heat radiators (6) are fixedly arranged at the bottom of the bearing plate (7); the temperature changing module (8) is detachably arranged in the bearing port, a plurality of placing ports (8011) are arranged at the top of the temperature changing module (8), and the top of the fixing frame (3) is provided with a camera (11) and an output end of the light source, wherein the camera (11) and the light source correspond to the bearing port.

2. The real-time fluorescent PCR detector of claim 1, wherein: and the side surface of the finned radiator (6) is fixedly provided with a fan (5) corresponding to the position of the finned radiator.

3. The real-time fluorescent PCR detector of claim 1, wherein: the side of the bearing plate (7) is fixedly provided with a rack (701), the side of the fixing frame (3) is fixedly provided with a sliding motor (4) corresponding to the rack (701), and the output end of the sliding motor (4) is fixedly provided with a gear meshed with the rack (701).

4. The real-time fluorescent PCR detector of claim 1, wherein: the temperature changing module (8) comprises a bottom plate (805) made of metal, a hollow temperature changing frame body (804) is fixedly arranged on the top surface of the bottom plate (805), a plurality of semiconductor refrigerators (803) are fixedly arranged in the hollow portion of the temperature changing frame body (804), a heating plate (802) is fixedly arranged on the top surface of each semiconductor refrigerator (803), a placing plate (801) is fixedly arranged on the top surface of each heating plate (802), and a plurality of placing openings (8011) are formed in the top surface of each placing plate (801).

5. The real-time fluorescent PCR detector of claim 4, wherein: the side surface of the temperature changing frame body (804) is provided with a locking device (806) used for locking with the side wall of the carrying port.

6. The real-time fluorescent PCR detector of claim 4, wherein: the side wall of the bottom plate (805) is connected with the finned radiator (6) through a plurality of radiating pipes (13).

7. The real-time fluorescent PCR detector of claim 1, wherein: the top of loading board (7) slides and sets up hot lid device (9), hot lid device (9) are including connecting plate (902), the top surface of connecting plate (902) and the output fixed connection of the vertical motor (901) of a plurality of, and connecting plate (902) set up slider (905) with the corresponding both sides of mount (3), set up slide rail (301) with slider (905) sliding connection on mount (3), the bottom of connecting plate (902) slides and sets up a plurality of slide bar (904), the bottom of slide bar (904) is fixed and is set up hot lid (903), and the spring is established to slide bar (904) overcoat, the both ends of spring offset with hot lid (903) and connecting plate (902) respectively.

8. The real-time fluorescent PCR detector of claim 1, wherein: the detection device is characterized in that a hollow light shield (2) is fixedly arranged at the top of the fixing frame (3), a detection port is formed in the bottom of the light shield (2), a filter wheel (14) is rotatably arranged in a cavity of the light shield (2), and a plurality of light filters are arranged on the filter wheel (14).

9. The real-time fluorescent PCR detector of claim 8, wherein: the top surface of the filter wheel (14) is fixedly connected with the rotating motor (12).

10. The real-time fluorescent PCR detector of claim 8, wherein: the cavity internal fixation of shell (1) sets up laser generator (16), the side of lens hood (2) sets up area source module (10), laser generator (16) are connected with area source module (10) through optic fibre (1601), the bottom surface of filter wheel (14) sets up a plurality of and the corresponding refractor of area source module (10).