CN218824063U - Single cell analysis system - Google Patents
Single cell analysis system Download PDFInfo
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- CN218824063U CN218824063U CN202222700344.6U CN202222700344U CN218824063U CN 218824063 U CN218824063 U CN 218824063U CN 202222700344 U CN202222700344 U CN 202222700344U CN 218824063 U CN218824063 U CN 218824063U
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- 238000004458 analytical method Methods 0.000 title claims description 23
- 239000011159 matrix material Substances 0.000 claims abstract description 7
- 230000003287 optical effect Effects 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 230000004075 alteration Effects 0.000 claims description 2
- 238000003384 imaging method Methods 0.000 claims 1
- 230000004907 flux Effects 0.000 abstract 1
- 238000000605 extraction Methods 0.000 description 11
- 238000010586 diagram Methods 0.000 description 7
- 238000013461 design Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010005003 Bladder cancer Diseases 0.000 description 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
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Abstract
The utility model provides a unicellular analytic system, unicellular analytic system includes: the first linear moving unit is used for providing linear movement in the horizontal direction for the clamping device; further comprising: the bearing unit is provided with a plurality of bearing positions distributed in a matrix form, and the bearing positions are used for bearing the ionization device; the bearing unit is arranged on the second linear moving unit, and the third linear moving unit comprises a first linear guide rail and a second linear guide rail which are respectively fixed on the bracket; the first driving module is used for driving the moving piece to move up and down on the first linear guide rail, and the moving piece is connected with the second linear moving unit; the counterweight is arranged on the second linear guide rail; the connecting rope is wound on the pulley, one end of the connecting rope is connected with the moving piece, and the other end of the connecting rope is connected with the counterweight piece. The utility model has the advantages of automation, high flux, etc.
Description
Technical Field
The utility model relates to a cell analysis, in particular to unicellular analytic system.
Background
Cells are the basic units constituting life bodies, and the change and behavior of each stage of a single cell in a complex and changeable environment are known, so that a method for analyzing the single cell is needed.
At present, the pretreatment work of the single cell analysis process is mainly completed manually, on one hand, the efficiency is low, on the other hand, the test result has larger fluctuation, and the poor test repeatability seriously influences the objectivity of the result analysis.
Current sample introduction designs generally have two approaches: the push-in type, which uses vacuum to draw a sample into a syringe from the outside, and the push-in type, which uses external pressure to press a sample into a syringe. The two modes both need to have strict requirements on the precision of a sampling needle and select a sampling mode, so that the precision and the flexibility are limited;
common automatic sample injectors mainly comprise a traditional disc type and an X-Y-Z gantry type, the two types have poor repeated positioning precision and single function, and the requirement for sample diversification cannot be met.
SUMMERY OF THE UTILITY MODEL
In order to solve the defects in the prior art, the utility model provides a unicellular analysis system.
The utility model aims at realizing through the following technical scheme:
the single cell analysis system comprises a clamping device, an ionization device, a first linear moving unit and an analyzer, wherein the clamping device is used for clamping the ionization device, the ionization device comprises Mao Xizhen, and the first linear moving unit is used for providing horizontal linear movement for the clamping device; the single-cell analysis system further comprises:
the bearing unit is provided with a plurality of bearing positions distributed in a matrix form, and the bearing positions are used for bearing the ionization device;
a second linear moving unit on which the carrying unit is disposed,
a third linear moving unit including,
the first linear guide rail and the second linear guide rail are respectively fixed on the bracket;
the first driving module is used for driving the moving piece to move up and down on the first linear guide rail, and the moving piece is connected with the second linear moving unit; the second linear moving unit and the third linear moving unit provide two-dimensional movement in a vertical plane for the ionization device on the bearing unit;
a counterweight disposed on the second linear guide rail;
the pulley and connect the rope, connect the rope and wind on the pulley, and one end is connected the moving member, the other end is connected the counterweight.
Compared with the prior art, the utility model discloses the beneficial effect who has does:
1. the applicability is good;
the ionization devices are arranged on the bearing unit in a matrix form and are used as independent consumables at one time, so that the precision and the flexibility of a sample are guaranteed, different samples can be suitable, and the requirements of multi-platform use and sample diversification are met;
2. the safety is good;
in the third linear moving unit, the running load is relieved by the design of the weight piece, and the safety factor of the product is greatly increased.
3. The positioning is accurate;
the design of the three-axis micro-motion platform compensates the processing and mounting errors, and ensures the high accuracy and the high repeatability of sample injection positioning;
the bearing piece is provided with a magnet which is matched with the ionization device in a magnetic attraction way, and the bearing piece is attracted by magnetic force, automatically centered and automatically positioned, so that the bearing piece is convenient and quick.
Drawings
The disclosure of the present invention will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only intended to illustrate the technical solution of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic diagram of a single cell analysis system according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a gripping device and a first linear moving unit according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a carrying unit according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a third linear motion unit according to an embodiment of the present invention;
fig. 5 is another schematic structural diagram of a third linear motion unit according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a micro-optical path module according to an embodiment of the present invention;
fig. 7 is a schematic structural diagram of an optical path module according to an embodiment of the present invention.
Detailed Description
Fig. 1-7 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and reproduce the invention. For explaining the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will appreciate variations or substitutions from these embodiments that will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Accordingly, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
fig. 1 is a schematic diagram showing a structure of a single-cell analysis system according to embodiment 1 of the present invention, and as shown in fig. 1, the single-cell analysis system includes:
the ionization device comprises a clamping device 12, an ionization device 36 and a first linear moving unit 1, wherein the clamping device 12 is used for clamping the ionization device 36, the ionization device 36 comprises Mao Xizhen, and as shown in fig. 2, the first linear moving unit 1 is used for providing linear movement in the horizontal direction for the clamping device 12;
the analyzer analyzes a sample to be detected by using technologies such as mass spectrometry and the like;
a carrying unit 40, as shown in fig. 3, the carrying unit 40 has a plurality of carrying positions distributed in a matrix form, and the carrying positions are used for carrying the ionizing device 36;
a second linear moving unit 28, the carrying unit 40 being provided on the second linear moving unit 28,
a third linear moving unit, as shown in fig. 4-5, the third linear moving unit comprising,
the device comprises a bracket 23, a first linear guide rail 24 and a second linear guide rail 22, wherein the first linear guide rail 24 and the second linear guide rail 22 are respectively fixed on the bracket 23;
a moving member 25 and a first driving module 27, wherein the first driving module 27 is used for driving the moving member 25 to move up and down on the first linear guide rail 24, and the moving member 25 is connected with the second linear moving unit 28; the second linear moving unit 28 and the third linear moving unit provide two-dimensional movement in a vertical plane for the ionization device 36 on the carrying unit 40;
a weight 16, the weight 16 disposed on the second linear guide 22;
the pulley 19 and connect the rope 20, connect the rope 20 and wind on the pulley 19, and one end connects the moving member 25, another end connects the counterweight 16.
In order to fix the ionization device 36 accurately, further, the carrier 34 is disposed obliquely at the carrier position;
a magnet 35 is arranged on the carrier 34 for fixing the ionization device 36 on the carrier 34, and correspondingly the gripper device 12 is arranged obliquely.
For accurate positioning, further, the gripping device 12 is provided on a three-axis micropositioner 10, and the three-axis micropositioner 10 is provided on the first linear moving unit 1.
In order to reduce the complexity and cost of the structure, the second linear guide 22 is a guide rod, and the weight 16 is sleeved on the guide rod.
The working mode of the single cell analysis system of the embodiment of the utility model comprises a changing stage of the ionization device 36, an extraction stage and a sample introduction stage; the ionizing device 36 is replaced by the following stages:
the first linear moving unit 1 provides the gripping device 12 with linear movement in the horizontal direction;
the second linear moving unit 28 provides the carrying unit 40 with a horizontal linear movement, and the third linear moving unit provides the second linear moving unit 28 with a vertical linear movement, so that the gripping device 12 grips the selected ionization device 36 and disengages from the carrying unit 40; a plurality of ionizing devices 36 are arranged on the carrying unit 40 in a matrix;
the third linear moving unit works in such a way that the first driving module 27 drives the moving member 25 to vertically move on the first linear guide rail 24, so as to drive the counterweight member 16 to move on the second linear guide rail 22 in a direction opposite to the moving direction of the moving member 25, and the connecting rope 20 connects the moving member 25 and the counterweight member 16 and bypasses the pulley 19; the second linear moving unit 28 is provided on the moving member 25.
In order to fix the ionization device 36 accurately, further, the carrier 34 is disposed obliquely at the carrier position;
a magnet 35 is arranged on the carrier 34 for fixing the ionization device 36 on the carrier 34, and correspondingly the gripper device 12 is arranged obliquely.
For accurate positioning, further, the gripping device 12 is provided on a three-axis micropositioner 10, and the three-axis micropositioner 10 is provided on the first linear moving unit 1.
In order to reduce the complexity and cost of the structure, the second linear guide 22 is a guide rod, and the weight 16 is sleeved on the guide rod.
Example 2:
according to the utility model discloses the application example of the unicell analysis system of embodiment 1 in the unicell analysis of bladder cancer.
In the present application example, as shown in fig. 1, the first linear motion unit 1 and the second linear motion unit 28 respectively employ an existing linear motion mechanism, such as a combination of a motor, a ball screw module, and a guide rail; the second linear moving unit 28 is fixed on the "L" -shaped moving member 25 and provides a horizontal back and forth movement for the carrying unit 40, and the third linear moving unit provides a vertical linear movement for the moving member 25;
as shown in fig. 2, a three-axis micropositioner 10 is arranged on a first linear moving unit 1, and realizes left-right movement in the horizontal direction, a connecting piece 11 is fixed on the three-axis micropositioner 10, and a clamping device 12 is obliquely fixed on the connecting piece 11 by adopting an electric clamping jaw;
as shown in fig. 3, the carrying unit 40 has a plurality of carrying positions distributed in a matrix form, the carrying member 34 is obliquely disposed at the carrying positions, and the magnet 35 is disposed on the carrying member 34 for magnetically attracting and cooperating with the ionizing device 36, so as to accurately position the ionizing device 36;
as shown in fig. 4-5, in the third linear moving unit, the moving member 25 is disposed on the first linear guide 24, the first driving module 27 employs a motor, a ball screw module, etc. to drive the moving member 25 to move up and down on the first linear guide 24, the lifting rings 26 are respectively disposed on the moving member 25 and the counterweight 16, the pulley 19 is disposed on the bracket 23, the connecting rope 20 passes around the pulley 19, and both ends are respectively connected with the lifting rings 26, so that the moving directions of the moving member 25 and the counterweight 16 are opposite;
the bearing unit 40 comprises a tray 29 and a sample plate 30, wherein the sample plate 30 is clamped in a groove of the tray 29, and moves back and forth in the horizontal direction in the groove under the pushing of external force.
As shown in fig. 6, the microscope optical path module 5 is composed of a movable stage 37, an optical path component 38, a fixed connection block 39 and a support frame 40; the movable object stage 37 is supported by the support frame 40, and the optical path component 38 is connected with and fixedly arranged on the fixed connecting block 39; the movable stage 37 moves along X, Y in the stroke range, i.e. moves two-dimensionally in the horizontal plane relative to the optical path component 38;
as shown in fig. 7, the optical path component 38 is composed of a CCD camera 41, a tube lens 42, a cube mirror 43, a liquid lens 44, and an objective lens 45; focusing is realized through a liquid lens 44, the direction of a light path is changed through a right-angle reflector 43, aberration is corrected through a tube lens 42, namely, an image is focused on an effective area of a CCD camera 41 behind, the image is received by the CCD camera 41 and transmitted to a processor, and the image is processed by the processor, so that cells to be extracted are accurately identified;
the recovery box 4 is used to recover the used ionization device 36, and the analyzer employs a mass spectrometer.
The single cell analysis system of this embodiment operates in a mode including an ionization device 36 replacement stage, an extraction stage, and a sample injection stage;
during the ionization device 36 replacement phase;
pulling out the sample plate 30, and fixing new ionization devices 36 on the carriers 34 obliquely by means of magnetic attraction; inserting the sample tray 30 into the tray 29;
the first linear moving unit 1 provides the gripping device 12 with linear movement in the horizontal direction;
the second linear moving unit 28 provides the carrying unit 40 with a horizontal linear movement, and the third linear moving unit provides the second linear moving unit 28 with a vertical linear movement, so that the gripping device 12 grips the selected ionization device 36 and disengages from the carrying unit 40;
the third linear moving unit works in such a way that the first driving module 27 drives the moving member 25 to vertically move on the first linear guide rail 24, so as to drive the counterweight member 16 to move on the second linear guide rail 22 in a direction opposite to the moving direction of the moving member 25;
in the extraction stage:
the first linear moving unit 1 drives the clamping device 12 (and the ionization device 36) to complete the single cell extraction and suction operations with the assistance of the microscopic light path module 5, specifically: adding an extraction liquid and cells on a movable objective table 37 in advance, moving a clamping device 12 carrying an empty ionization device 36 to the position above the extraction liquid on the movable objective table 37, driving the clamping device 12 to descend along Z by a three-axis micro-motion table 10 to absorb the extraction liquid, then driving the clamping device 12 to ascend along Z by the three-axis micro-motion table 10 to a certain height, moving the movable objective table 37 along X to move the cells to the position below an electric clamping jaw 12, and completely dripping the extraction liquid in the ionization device 36 on the cells by the clamping device 12 descending along Z above a light path component 38 to finish extraction; the extraction process provides image recognition of the cells by the optical path assembly 38;
after extraction, the extracted sample is sucked by the ionization device 36, and the clamping device 12 rises along the Z direction;
in the sampling stage:
the first linear moving unit 1 drives the clamping device 12 (the ionization device 36) to enable a capillary needle outlet of the ionization device 36 to correspond to a sample inlet of an analyzer, the deviation is compensated through the triaxial micropositioner 10, high pressure is applied, and a liquid-phase sample in Mao Xizhen forms electrospray and enters the sample inlet;
thereafter, the first rectilinear motion unit 1 drives the clamp device 12 (and the ionization device 36), the ionization device 36 reaches the upper side of the recovery box 4, the clamp device 12 is released, and the used ionization device 36 falls into the recovery box 4.
Claims (7)
1. The single cell analysis system comprises a clamping device, an ionization device, a first linear moving unit and an analyzer, wherein the clamping device is used for clamping the ionization device, the ionization device comprises Mao Xizhen, and the first linear moving unit is used for providing horizontal linear movement for the clamping device; characterized in that the single cell analysis system further comprises:
the bearing unit is provided with a plurality of bearing positions distributed in a matrix form, and the bearing positions are used for bearing the ionization device;
a second linear moving unit on which the bearing unit is disposed,
a third linear moving unit including,
the first linear guide rail and the second linear guide rail are respectively fixed on the bracket;
the first driving module is used for driving the moving piece to move up and down on the first linear guide rail, and the moving piece is connected with the second linear moving unit; the second linear moving unit and the third linear moving unit provide two-dimensional movement in a vertical plane for the ionization device on the bearing unit;
the counterweight is arranged on the second linear guide rail;
the pulley and connect the rope, it twines to connect the rope on the pulley, and one end is connected the moving member, the other end is connected the counterweight.
2. The single-cell analysis system according to claim 1, wherein a carrier is obliquely disposed at the carrier position;
the magnet is arranged on the bearing piece and used for fixing the ionization device on the bearing piece; the clamping device is obliquely arranged.
3. The single cell analysis system of claim 2, wherein the grasping device is disposed on a three-axis micropositioner disposed on the first linear motion unit.
4. The single cell analysis system of claim 1, wherein the second linear guide is a guide rod, and the weight member is fitted over the guide rod.
5. The single cell analysis system of claim 1, wherein the grasping device is a motorized jaw.
6. The single cell analysis system of claim 1, further comprising a micro optical path module comprising a mobile stage, an optical path assembly, a fixed connection block, and a support frame; the two-dimensional movable object stage is supported by the support frame, and the light path component is connected with the fixed connecting block and is fixedly arranged; the optical path component is used for imaging cells.
7. The single-cell analysis system of claim 6, wherein the optical path assembly comprises a camera, a tube lens, a cube-corner mirror, a liquid lens, and an objective lens, arranged in sequence; the liquid lens is used for realizing focusing, and the tube lens is used for correcting aberration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222700344.6U CN218824063U (en) | 2022-10-14 | 2022-10-14 | Single cell analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222700344.6U CN218824063U (en) | 2022-10-14 | 2022-10-14 | Single cell analysis system |
Publications (1)
| Publication Number | Publication Date |
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| CN218824063U true CN218824063U (en) | 2023-04-07 |
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|---|---|---|---|
| CN202222700344.6U Active CN218824063U (en) | 2022-10-14 | 2022-10-14 | Single cell analysis system |
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| CN (1) | CN218824063U (en) |
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- 2022-10-14 CN CN202222700344.6U patent/CN218824063U/en active Active
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Address after: West side of 1st floor, 1st floor, Building A, No. 288 Jingu Middle Road (East), Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee after: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region after: China Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee before: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region before: China |
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