CN218811735U - Microorganism directly scribbles device - Google Patents
Microorganism directly scribbles device Download PDFInfo
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- CN218811735U CN218811735U CN202223042639.5U CN202223042639U CN218811735U CN 218811735 U CN218811735 U CN 218811735U CN 202223042639 U CN202223042639 U CN 202223042639U CN 218811735 U CN218811735 U CN 218811735U
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Abstract
The utility model discloses a microorganism directly scribbles device, include: the hollow first section body is used for being connected with an external gun head; the second section body is positioned below the first section body and fixedly connected with the first section body, the bottom of the second section body is arranged in a hollow structure, and the inner wall of the bottom of the second section body is provided with a bulge; the coating head is provided with a coating section and a mounting section, the coating section is positioned outside the second section body, the mounting section axially extends into the bottom of the second section body, the outer wall of the mounting section is abutted against the inner wall of the bottom of the second section body, and the mounting section is provided with a groove matched with the protrusion to fix the coating head and the second section body. The utility model discloses a mutually supporting buckle between arch and the recess for the mounting structure of scribbling the head is more firm, and assembly structure is stable, can not lead to scribbling rocking of head during the coating.
Description
Technical Field
The utility model relates to a life science instrument technical field, concretely relates to microorganism directly scribbles device.
Background
Currently, the most common method in the preparation of mass spectrometric samples of microorganisms is the direct coating method, in which colonies of microorganisms (bacteria, molds, yeasts, etc.) cultured on agar medium or any other medium in a petri dish are sampled using tools that can be sterilized or disposable tools such as inoculating loop (oese), stick, tube or conical tube, and then uniformly coated on a target plate. However, the colonies of microorganisms grown on the petri dish are very irregular and soft, making the mass spectrometric direct spotting process of microorganisms difficult to succeed.
Patent CN114752477A discloses an automatic directly scribble device, including the sleeve pipe, the activity of sleeve pipe below is provided with the probe, the probe bottom can with directly scribble the fixed cup joint of head, in order to solve above-mentioned technical problem, this technical scheme is that the outer bottom cladding of head is scribbled directly has aseptic cotton or dustless cloth.
However, the straight coating head of this structure still has some technical problems, for example, the aseptic cotton or the dust-free cloth at the outer bottom of the straight coating head is easy to fall off, and there is a possibility of contamination by the external environment when stored or used.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model provides a microorganism direct coating device which has simple and convenient direct coating sample application operation, reasonable structure and stable assembly.
The utility model provides a technical scheme that its technical problem adopted is: a microbial direct application device comprising:
the hollow first section body is used for being connected with an external gun head;
the second section body is positioned below the first section body and fixedly connected with the first section body, the bottom of the second section body is arranged in a hollow structure, and a bulge is arranged on the inner wall of the bottom of the second section body;
the coating head is provided with a coating section and a mounting section, the coating section is positioned outside the second section body, the mounting section axially extends into the second section body, the outer wall of the mounting section abuts against the inner wall of the second section body, and the mounting section is provided with a groove matched with the protrusion to fix the coating head and the second section body.
Furthermore, the cross section of the coating section is of a circular arc structure.
Further, the acute angle included angle between the upper end surface of the coating section and the vertical direction is alpha, and alpha satisfies the following conditions: alpha is more than or equal to 40 degrees and less than or equal to 80 degrees.
Further, the height of the protrusion along the direction of the inner wall of the second segment body is a, the width of the protrusion along the direction perpendicular to the inner wall of the second segment body is b, and a and b satisfy: a/b is more than or equal to 0.5 and less than or equal to 2.
Furthermore, the number of the bulges is at least one, and the bulges are uniformly distributed at the same axial height of the inner wall of the second section body; alternatively, the protrusion is an annular protrusion.
Further, the bottom distance between the protrusion and the second segment body is c, the height of the installation segment is h, and the distance between c and h satisfies the following conditions: c/h is more than or equal to 0.2 and less than or equal to 0.6.
Furthermore, the second segment body is of a conical structure with a large upper part and a small lower part, and the second segment body is arranged in a hollow structure.
Furthermore, the coating head is made of silica gel and polyethylene.
Furthermore, the upper end of the first section body and the inner wall thereof are in smooth transition through an arc, and the inner diameter of the first section body is reduced from top to bottom.
Furthermore, a plurality of reinforcing ribs are arranged on the outer side wall of the first section body.
The utility model has the advantages that: the coating section positioned outside the second section body can dip the microorganisms in the culture dish and coat the microorganisms on the target plate; the mounting section of the coating head is arranged in the second section body, and meanwhile, the coating head is firmly connected with the second section body through the mutual clamping of the bulge on the inner wall of the second section body and the groove on the mounting section, so that the coating head is prevented from falling off during coating, and the coating effect and quality are ensured; wherein, because the material of the coating head selects a high-activity adsorption material, the adsorption is strong, can adsorb many kinds of substances, therefore is used in the direct coating device of the utility model, can increase the adsorption capacity to the microorganism, guarantee the effect of coating; the cross section of coating section can increase area of contact for the circular arc type structure, dips in and gets more microorganism, and also can coat when coating the target board with the microorganism more even, and the circular arc type structure does not have the edges and corners moreover, has reduced the possibility of scraping colored target board when the coating. Note that, the "bottom of the second segment" referred to herein means an end of the second segment away from the first segment; and the distance c between the bulge and the bottom end of the second section body refers to the height from the central axis of the bulge to the bottom end of the second section body.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the description below are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without inventive labor.
FIG. 1 is a perspective view of a microbial direct-coating device according to an embodiment of the present invention;
FIG. 2 is a front view of a microbial direct application device according to an embodiment of the present invention;
FIG. 3 is a partially enlarged view of the microbial direct application device shown in FIG. 2;
FIG. 4 is a cross-sectional view of the microbial direct application device shown in FIG. 2;
FIG. 5 is a partially enlarged view of the microbial direct application device shown in FIG. 4;
fig. 6 is a front view of a coating head according to an embodiment of the present invention.
1-first segment body, 2-second segment body, 21-bottom, 211-projection, 3-coating head, 31-coating segment, 32-mounting segment and 321-groove.
Detailed Description
In order to make the technical solutions of the present invention better understood, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts shall fall within the protection scope of the present invention.
The first embodiment is as follows:
as shown in fig. 1-6, a microorganism direct coating device comprises a first section 1 which is a hollow structure, a second section 2 fixed below the first section, and the second section 2 also is a hollow structure. In the present embodiment, the first segment 1 and the second segment 2 are integrally formed. Further, the inner wall of the bottom 21 of the second segment 2 is provided with two protrusions 211, specifically in this embodiment, the number of the protrusions 211 is two, two the protrusions 211 are located at the same axial height, and are uniformly arranged on the inner wall of the second segment 2 at intervals. The coating head 3 has a coating section 31 and a mounting section 32, and the mounting section 32 has a groove 321 on the outer side wall. When the mounting section 32 axially extends into the bottom 21 of the second body 2, the outer wall of the mounting section 32 abuts against the inner wall of the bottom 21 of the second body 2, and the groove 321 and the protrusion 211 are engaged with each other, so that the painting head 3 is firmly fixed in the second body 2. The coating section 31 dips the bacterial colony in the culture dish and coats the bacterial colony on the target plate to complete the direct-coating bacteria selection process.
The cross section of coating section 31 is the circular arc type structure, can increase the area of contact with culture dish or target plate, increases the efficiency of directly scribbling and choosing the fungus, and the circular arc type structure can avoid producing stress concentration phenomenon moreover, has guaranteed coating section 31's intensity. Furthermore, the coating section 31 with the arc-shaped structure can also avoid scratching the target plate in the dipping and coating processes.
In this embodiment, an acute included angle between the upper end surface of the coating section 31 and the vertical direction is α, and if α is less than 40 °, a larger gap exists between the upper end surface of the coating section 31 and the bottom end of the second section 2, and when the coating section 31 is coated on the target board, the coating section 31 may shake due to lack of support, resulting in poor coating effect; if alpha is more than 80 degrees, enough space is not provided between the coating section 31 and the bottom end of the second section body 2, when the coating section 31 is coated, the coating section 31 deforms due to contact with a target plate, so that the upper end surface of the coating section 31 is abutted against the bottom end of the second section body 2, the pressure applied to the coating section 31 is transmitted to the second section body 2, the whole microorganism direct coating device is in a stressed state, the strength of the microorganism direct coating device is influenced, and the direct coating bacteria picking operation is not facilitated; only when alpha is more than or equal to 40 degrees and less than or equal to 80 degrees, the bottom end of the second segment body 2 and the upper end surface of the coating segment 31 are provided with a proper gap, when the coating segment 31 is extruded, the deformation space is provided, the stress condition of the second segment body 2 is reduced, and the better coating effect is ensured.
The height of the protrusion 211 along the inner wall of the second segment 2 is a, the width along the direction perpendicular to the inner wall of the second segment 2 is b, if a/b is less than 0.5, the height of the protrusion 211 is too low, so that the engaging height between the protrusion 211 and the groove 321 on the mounting segment 32 is small, and the fixing effect between the coating head 3 and the second segment 2 is poor; if a/b is greater than 2, the width of the protrusion 211 is too small, i.e. the engagement depth between the protrusion 211 and the groove 321 is shallow, and the fixing effect is not good; only when a/b is more than or equal to 0.5 and less than or equal to 2, the height and the width of the bulge 211 are both moderate, and the fixing effect between the coating head 3 and the second section body 2 is ensured.
Further, the distance between the central axis of the protrusion 211 and the bottom end of the second segment 2 is c, and the height of the mounting segment 32 is h. If c/h is less than 0.2, the height of the protrusion 211 from the bottom end of the second segment 2 is smaller, and the height of the installation segment 32 is higher, that is, the protrusion 211 is located below the middle of the installation segment 32, when the coating segment 31 is coated on the target plate, the installation segment 32 is prone to shaking, so that the fixing effect of the coating head 3 and the second segment 2 is poor; if c/h is more than 0.6, the projection 211 is positioned above the middle of the mounting section 32, and the fixing effect of the coating head and the second section body 2 is poor; only when c/h is more than or equal to 0.2 and less than or equal to 0.6, the second section body 2 has better buckling effect of the coating head 3. Preferably, the fixing effect between the coating head 3 and the second segment 2 is best when c/h = 0.5.
The second segment 2 is a tapered structure with a large top and a small bottom in this embodiment, so that the diameter of the bottom 21 of the second segment 2 is small, and success can be guaranteed when the second segment is dipped in bacterial colonies and coated on a target plate with a small area. In order to save raw materials and reduce the production and manufacturing cost, the second section body 2 is of a hollow structure.
In this embodiment, in order to ensure that the external lance head can be smoothly inserted into the first segment body 1, the upper end surface of the first segment body 1 and the inner wall thereof are smoothly transited through an arc, that is, when the lance head is inserted into the first segment body, the smooth arc can play a role in guiding, and the arc structure can also reduce the phenomenon of stress concentration, thereby playing a role in enhancing the strength of the first segment body 1. Further, in order to guarantee the clamping firmness between the gun head and the first section body 1 without using an external fixing piece, the inner diameter of the first section body 1 is reduced from top to bottom, namely, the gun head is gradually wrapped firmly and clamped.
In order to increase the strength of the microorganism direct coating device, a plurality of ribs (not shown in the figure) are arranged on the outer side wall of the first segment 1 which is in contact with the external gun head.
The material of scribbling head 3 of this embodiment is silica gel, and silica gel is a high active adsorption material, has open porous structure, and the adsorptivity is strong, can adsorb multiple material, consequently is used for the utility model provides a directly scribble on the device, can increase the adsorption efficiency to the microorganism, guarantee the effect of coating. Of course, the material of the head 3 may be other materials having adsorption ability, such as polyethylene.
In the embodiment, the manual direct coating device and the microorganism direct coating device in the technical scheme are assembled, that is, a gun head of the manual direct coating device is inserted into the first segment body 1 to form a microorganism sampling device; the second section 2 of the microorganism direct-coating device is inserted into a culture medium and is contacted with bacterial colonies in the culture medium, and the bacterial colonies can be adhered to the coating section 31 of the coating head 3; then, coating the coating section 31 adhered with the bacterial colony on a target plate to finish sampling; once the coating is complete, the microbial direct application device is removed from the manual direct application device and thrown into a trash can. Meanwhile, a gun head and an inoculating loop on the market are used for colony coating and used as a control test to detect the coating effect.
The species of the bacteria were identified using MALDI-TOF mass spectrometer, and the identification was directly related to the mass of the colony coating on the target plate.
TABLE 1 test results
As can be seen from the test results of table 1 above: the manual direct coating device is combined with the microorganism direct coating device in the technical scheme to form a microorganism sampling device, different bacteria are coated and sampled, the identification performance of a mass spectrometer is excellent, the analysis result of the strains can also reach 99.9 percent, and all species are correctly identified; and the microorganism sampling device formed by the manual direct coating device and the gun head or the inoculating loop is used for coating and sampling, the identification performance of different bacteria is poor, and the identification accuracy of species is greatly reduced. Therefore, the technical scheme is used for coating the bacterial colony, so that the coating efficiency is ensured, and the coating quality is also ensured.
Example two:
in this embodiment, the protrusion is an annular protrusion formed around the inner wall of the second segment 2, and correspondingly, the groove is an annular groove formed around the outer wall of the mounting segment 32, so that the connection between the coating head 3 and the second segment 2 is more firm and reliable due to the structure.
The above is to the microorganism direct coating device that the utility model provides introduces in detail, to the general technical personnel in this field, according to the utility model discloses the thought, all has the change part on concrete implementation and application scope, to sum up, this description content should not be understood as right the utility model discloses a restriction.
Claims (10)
1. A microbial direct application device, comprising:
the hollow first section body is used for being connected with an external gun head;
the second section body is positioned below the first section body and fixedly connected with the first section body, the second section body is arranged in a hollow structure, and a bulge is arranged on the inner wall of the bottom of the second section body;
the coating head is provided with a coating section and a mounting section, the coating section is positioned outside the second section body, the mounting section axially extends into the second section body, the outer wall of the mounting section is abutted against the inner wall of the second section body, and the mounting section is provided with a groove matched with the protrusion so as to fix the coating head and the second section body.
2. The microbial direct application device of claim 1, wherein: the cross section of the coating section is of an arc-shaped structure.
3. The microbial direct application device of claim 2, wherein: the acute angle included angle between the upper end surface of the coating section and the vertical direction is alpha, and alpha satisfies the following conditions: alpha is more than or equal to 40 degrees and less than or equal to 80 degrees.
4. The microbial direct application device of claim 1, wherein: the height of the protrusion in the direction of the inner wall of the second segment body is a, the width of the protrusion in the direction perpendicular to the inner wall of the second segment body is b, and a and b satisfy the following conditions: a/b is more than or equal to 0.5 and less than or equal to 2.
5. The microbial direct application device of claim 1, wherein: the number of the bulges is at least one, and the bulges are uniformly distributed at the same axial height of the inner wall of the second section body; alternatively, the protrusion is an annular protrusion.
6. The microbial direct application device of claim 1, wherein: the bottom distance of arch and the second section body is c, the height of installation section is h, satisfies between c and the h: c/h is more than or equal to 0.2 and less than or equal to 0.6.
7. The microbial direct application device of claim 1, wherein: the second segment body is in a conical structure with a large upper part and a small lower part.
8. The microbial direct application device of claim 1, wherein: the coating head is made of silica gel and polyethylene.
9. The microbial direct application device of claim 1, wherein: the upper end face of the first section body is in smooth transition with the inner wall of the first section body through an arc, and the inner diameter of the first section body is reduced from top to bottom.
10. The microbial direct application device of claim 9, wherein: and a plurality of reinforcing ribs are arranged on the outer side wall of the first section body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223042639.5U CN218811735U (en) | 2022-11-16 | 2022-11-16 | Microorganism directly scribbles device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223042639.5U CN218811735U (en) | 2022-11-16 | 2022-11-16 | Microorganism directly scribbles device |
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| Publication Number | Publication Date |
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| CN218811735U true CN218811735U (en) | 2023-04-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202223042639.5U Active CN218811735U (en) | 2022-11-16 | 2022-11-16 | Microorganism directly scribbles device |
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