CN218811660U

CN218811660U - Culture plate supporting device and automatic liquid transfer workstation

Info

Publication number: CN218811660U
Application number: CN202223212319.XU
Authority: CN
Inventors: 曹林; 唐波; 占景松; 龚小山
Original assignee: Vazyme Medical Co ltd; Nanjing Novozan Biotechnology Co ltd
Current assignee: Vazyme Medical Co ltd; Nanjing Novozan Biotechnology Co ltd
Priority date: 2022-11-30
Filing date: 2022-11-30
Publication date: 2023-04-07
Anticipated expiration: 2032-11-30

Abstract

The utility model discloses a cultivate board strutting arrangement and automatic liquid-transfering workstation belongs to biological sample pretreatment and preparation instrument technical field, for solving current liquid-transfering workstation and move liquid not thorough scheduling problem design. The utility model discloses a cultivate board strutting arrangement includes: a base; and the vertical elastic pieces are symmetrically arranged on the base, the top ends of the vertical elastic pieces directly or indirectly protrude out of the upper surface of the base, and the vertical elastic pieces are configured to provide elastic force in the vertical direction. The utility model discloses a cultivate board strutting arrangement and automatic liquid-transfering work station, cultivate board bottom and contact vertical elastic component earlier before contacting the base, can self-adaptively finely tune because of the suction head terminal surface that loading difference or machining precision lead to and the difference in height between the cultivation board, can contact the diapire that liquid held the chamber when guaranteeing that the suction head descends to the extreme lower position to can thoroughly suction liquid holds the liquid in the chamber, even if the small volume liquid measure also can be sucked futilely clean.

Description

Culture plate supporting device and automatic liquid transfer workstation

Technical Field

The utility model relates to a biological sample pretreatment and preparation instrument technical field especially relate to cultivate board strutting arrangement and including this cultivate board strutting arrangement's automatic liquid-transfering work station.

Background

In many fields of life science and technology, for example, nucleic acid extraction (plasmid DNA, genomic DNA, RNA), library construction, ELISA experiments and results, system construction such as PCR and flow, sample pretreatment, and compound screening (molecules, cells, microorganisms, nematodes) require liquid treatment such as liquid culture, detection, and pipetting using a device such as a high-throughput automated pipetting station.

The automatic pipetting workstation mainly controls a multi-channel (96-channel or 8-channel) pipettor to move among a standard plate position, a vibration incubation position, a magnetic frame position, a PCR position and a waste pipette tip position through an automatic control system, and the pipetting is completed by utilizing a disposable pipetting pipette tip loaded at the lower end part of the pipettor.

Because of the difference of manufacturers and the limitation of processing precision, there may be a large size difference between the culture plate (or called 96-well plate) used for culturing liquid and the suction head, when the liquid transfer head loaded with the suction head is lowered into the well site of the 96-well plate for liquid absorption, the bottom end of the suction head may not contact the bottom surface of the well site, resulting in incomplete absorption of the bottom liquid of part of the well site, which is especially serious when small volumes of liquid are processed.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cultivate board strutting arrangement and automatic move liquid workstation, move the liquid more thoroughly, avoid having remaining.

To achieve the purpose, on one hand, the utility model adopts the following technical scheme:

a culture plate support device comprising: a base; and a plurality of vertical elastic pieces symmetrically arranged on the base, wherein the top ends of the vertical elastic pieces directly or indirectly protrude out of the upper surface of the base, and the vertical elastic pieces are configured to provide elastic force in the vertical direction.

In a preferred embodiment, the culture plate support device further comprises a plurality of lateral elastic members symmetrically disposed on the base, the lateral elastic members being configured to provide an elastic force in a horizontal direction.

In a preferred embodiment, the transverse elastic member comprises a mounting part and an elastic part which are connected, and the elastic part is formed by bending a metal sheet.

In a preferred embodiment, the base is provided with a limit stop, and the elastic part elastically deforms along the limit stop.

In one preferred embodiment, the culture plate supporting device further comprises a key cap, a key cover plate and a connecting member, the vertical elastic member is arranged in the through hole of the key cap in a penetrating manner, the key cap is arranged in the through hole of the key cover plate in a penetrating manner, and the key cover plate is connected to the base through the connecting member.

In one preferred embodiment, the vertical elastic member is a spring.

In a preferred embodiment, the support device further comprises a cylindrical guide post, which is fastened to the top end of the vertical elastic member.

In a preferred embodiment, a plurality of culture plate positioning members are symmetrically arranged on the base, and all the culture plate positioning members surround a culture plate placing area.

In one preferred embodiment, the culture plate positioning member is bent at a right angle, and the vertical elastic member is arranged at the top corner of the inner side of the culture plate positioning member.

On the other hand, the utility model adopts the following technical scheme:

automatic move liquid workstation, including the culture plate, still include foretell culture plate strutting arrangement, the culture plate is placed on culture plate strutting arrangement's the vertical elastic component.

The utility model discloses a culture plate strutting arrangement includes base and a plurality of vertical elastic component, the top protrusion of vertical elastic component is in the upper surface of base, the culture plate bottom contacts vertical elastic component earlier before contacting the base, can finely tune because of loading difference or the difference in height between the suction head terminal surface that the machining precision leads to and the culture plate adaptively, can contact the diapire that liquid held the chamber when guaranteeing the suction head to descend to the lowest position, thereby can thoroughly suction liquid hold the liquid in the chamber, even if the small volume liquid measure also can be sucked completely.

The utility model discloses an automatic move liquid workstation includes foretell culture plate strutting arrangement, can finely tune because of loading difference or the difference in height that machining precision leads to adaptively to thorough suction liquid holds the liquid in the chamber, has the residue after avoiding moving the liquid, is particularly useful for the liquid work that moves of small volume liquid measure.

Drawings

FIG. 1 is a schematic view of a plate support device according to an embodiment of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1 at A;

FIG. 3 is an exploded view of a plate support apparatus according to an embodiment of the present invention;

FIG. 4 is a top view of a plate support apparatus according to an embodiment of the present invention;

FIG. 5 is a schematic view of another embodiment of the present invention;

FIG. 6 is a partial enlarged view at B in FIG. 5;

FIG. 7 is an exploded view of another embodiment of the present invention;

FIG. 8 is a top view of another embodiment of the present invention;

fig. 9 is a schematic structural view of a transverse elastic member according to an embodiment of the present invention.

In the figure:

1. a base; 2. a vertical elastic member; 3. a transverse elastic member; 4. a limit stop block; 5. a culture plate positioning member; 6. a key cap; 7. a key cover plate; 8. a connecting member; 9. a guide post; 31. an installation part; 32. an elastic part; 33. and installing the through hole.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature "under," "beneath," and "under" a second feature may be directly under or obliquely under the second feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. As used herein, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are for purposes of illustration only and do not denote a single embodiment.

This embodiment discloses cultivate board strutting arrangement and including this cultivate board strutting arrangement's automatic liquid-transfering work station, and the table surface of automatic liquid-transfering work station includes a plurality of cultivation boards, a magnetic force frame position, one and shakes and incubate position, two heating refrigeration positions, a PCR (polymerase chain reaction) position, a useless suction head position (be used for depositing the abandonment suction head), and the cultivation board is placed on cultivation board strutting arrangement. As shown in fig. 1 to 8, the culture plate supporting device includes a base 1 and a plurality of vertical elastic members 2, and the plurality of vertical elastic members 2 are symmetrically disposed on the base 1. The top end of the vertical elastic member 2 protrudes directly or indirectly from the upper surface of the base 1, the vertical elastic member 2 is configured to provide an elastic force in the vertical direction, and the culture plate is placed on the vertical elastic member 2 of the culture plate supporting device. The other structures of the culture plate and the automated pipetting station are the same as the prior art and are not described in detail here.

The culture plate may be, but is not limited to, a 96-well plate, and in the present embodiment, a 96-well plate is exemplified. The top of vertical elastic component 2 directly or indirectly protrudes from the upper surface of base 1, the bottom of suction head box or 96-hole plate contacts vertical elastic component 2 before contacting base 1, the height of the top of vertical elastic component 2 protruding from the upper surface of base 1 is the distance allowing the 96-hole plate to move up and down, the height difference between the end face of the suction head and the 96-hole plate caused by loading difference or processing precision can be self-adaptively fine-adjusted, the suction head can contact the bottom wall of the liquid containing cavity on the 96-hole plate when descending to the lowest position, and therefore liquid in the liquid containing cavity can be thoroughly sucked out, and the liquid can be sucked completely even if the liquid with small volume is completely sucked.

At the initial stage of pipetting, the suction head falls, the end face of the suction head is contacted with the bottom wall of the liquid containing cavity to push the 96-hole plate to continue moving downwards for a certain distance, and the vertical elastic piece 2 contracts until the suction head falls to the lowest position. After the suction is carried out for a period of time, the suction head is lifted upwards, the 96-hole plate is pushed by the vertical elastic piece 2 to move upwards along with the suction head, the contact state is still kept between the end surface of the suction head and the bottom wall of the liquid containing cavity at the moment, the liquid in the liquid containing cavity can be continuously sucked out, the situation that the experimental result is inaccurate due to liquid loss is reduced.

As shown in fig. 5 to 8, the culture plate support device further includes a plurality of transverse elastic members 3 symmetrically disposed on the base 1, the transverse elastic members 3 are configured to provide an elastic force in a horizontal direction, when there is an angular deviation between the 96-well plate or the tip cassette, the transverse elastic members 3 can push the 96-well plate or the tip cassette within a certain range, so as to achieve a self-adaptive adjustment of the 96-well plate or the tip cassette, solve a problem that a part of the well sites of the 96-well plate (or the tip cassette) are not completely absorbed due to the angular deviation between the 96-well plate (or the tip cassette) and offset an influence of the dimensional deviation in the peripheral direction of the tip cassette and the 96-well plate on the positioning accuracy of the pipettor the tip.

The specific structure of the lateral elastic member 3 is not limited, and it may be elastically deformed in the horizontal direction to provide the thrust force. In this embodiment, as shown in fig. 9, the lateral elastic member 3 includes a mounting portion 31 and an elastic portion 32 connected to each other, a mounting through hole 33 is formed in the mounting portion 31, and a bolt (not shown) passes through the mounting through hole 33 to fix the mounting portion 31 to the base 1. The elastic part 32 is formed by bending a metal sheet, the contraction and reset directions of the elastic part 32 are shown by the straight line with an arrow in fig. 9, the elastic value is stable, the elastic part can bear multiple times of compression deformation and reset, and the service life is long. It should be noted that the elastic portion 32 may also be other elements capable of providing elastic force, such as a rubber pad, a silicone pad, etc.

As shown in fig. 5 to 8, the base 1 is provided with a limit stop 4, and the elastic portion 32 can be elastically deformed along the limit stop 4, so that the elastic portion 32 does not move laterally when being compressed and reset, and the elastic force is not weakened. The specific shape of the limit stop 4 is not limited, and in the embodiment, one surface of the limit stop 4 facing the 96-well plate is an inclined surface, so that the culture plate can be prevented from being taken and placed. In addition, the limit stop 4 can guide the culture plate when the culture plate is placed in the culture plate supporting device.

As shown in fig. 1 to 4, the supporting device of the cultivation plate further comprises a key cap 6, a key cover plate 7 and a connecting piece 8, the vertical elastic piece 2 is arranged in a through hole of the key cap 6 in a penetrating manner, the key cap 6 is arranged in a through hole of the key cover plate 7 in a penetrating manner, and the key cover plate 7 is connected to the base 1 through the connecting piece 8. The top protrusion of vertical elastic component 2 can butt on the top of vertical elastic component 2 when pressing key cap 6 and key cover 7, 96 orifice plate or suction head box place on the base 1, and the elastic force of vertical elastic component 2 can direct action on 96 orifice plate or suction head box, and the position of 96 orifice plate or suction head box is more accurate. The vertical elastic piece 2 can be but not limited to a spring, a rubber pad or a silica gel pad, and the product is mature and low in cost.

The installation method of the vertical elastic element 2 is not limited to the use of the key cap 6 and the key cover 7, and as shown in fig. 5 to 8, the culture plate supporting device further comprises a cylindrical guide post 9, and the guide post 9 is buckled on the top end of the vertical elastic element 2. Compared with the top end of the vertical elastic piece 2, the top area of the guide post 9 is larger and smoother, and the 96-hole plate or the suction head box is more stable when placed on the top surface of the guide post 9, so that liquid transfer is smoother. Of course, it is also feasible to design the guide post 9 into a tubular shape, and the top end of the vertical elastic component 2 protrudes from the top end of the guide post 9, and in this structure, the guide post 9 mainly plays a role in guiding, so as to prevent the vertical elastic component 2 from moving along the radial direction.

As shown in FIGS. 1 to 8, a plurality of culture plate positioning members 5 are symmetrically provided on a base 1, and all the culture plate positioning members 5 enclose a culture plate placement area. Cultivate board setting element 5 and can carry out the coarse positioning to 96 orifice plates or suction head box, for the accurate location of follow-up vertical elastic component 2 and horizontal elastic component 3 prepares, work efficiency is high, and the culture plate position is more accurate.

The specific shape of the culture plate positioning member 5 is not limited, and it is sufficient to position a 96-well plate or a tip cassette. The top view projection of the culture plate in the embodiment is rectangular, the culture plate positioning pieces 5 used for fixing the periphery of the culture plate are in right-angle bending shapes, and the vertical elastic pieces 2 are arranged at the top corners of the inner sides of the culture plate positioning pieces 5. The 96-well plate is usually rectangular, and the culture plate positioning piece 5 which is in a right-angled bent shape can be clamped at the outer side of the vertex angle of the 96-well plate, so that the transverse shaking of the 96-well plate in the pipetting process can be avoided, and the pipetting process can be smoothly executed. In the case of a plate having another shape, the shape of the plate holder 5 may be changed accordingly, and the plate may be fixed.

Note that the above description is only for the preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.

Claims

1. Culture plate strutting arrangement, its characterized in that includes:

a base (1); and (c) a second step of,

the elastic device comprises a plurality of vertical elastic pieces (2) which are symmetrically arranged on the base (1), wherein the top ends of the vertical elastic pieces (2) directly or indirectly protrude out of the upper surface of the base (1), and the vertical elastic pieces (2) are configured to provide elastic force in the vertical direction.

2. The culture plate support device according to claim 1, further comprising a plurality of lateral elastic members (3) symmetrically arranged on the base (1), the lateral elastic members (3) being configured to provide an elastic force in a horizontal direction.

3. The support device according to claim 2, wherein the lateral spring member (3) comprises a mounting portion (31) and a spring portion (32) connected, the spring portion (32) being formed by bending a metal sheet.

4. A support device according to claim 3, wherein the base (1) is provided with a limit stop (4), the resilient portion (32) being resiliently deformable along the limit stop (4).

5. The growth plate support device according to any of claims 1 to 4, further comprising a key cap (6), a key cover plate (7) and a connector (8), wherein the vertical elastic member (2) is inserted into a through hole of the key cap (6), the key cap (6) is inserted into a through hole of the key cover plate (7), and the key cover plate (7) is connected to the base (1) through the connector (8).

6. The growth plate support device according to claim 5, characterized in that the vertical elastic member (2) is a spring.

7. The device according to any of claims 1 to 4, further comprising a cylindrical guide post (9), wherein the guide post (9) is snap-fitted on top of the vertical spring (2).

8. A culture plate support device according to any of claims 1-4, wherein a plurality of culture plate positioning elements (5) are symmetrically arranged on the base (1), all culture plate positioning elements (5) enclosing a culture plate placement area.

9. The growth plate support device according to claim 8, wherein the growth plate positioning member (5) is bent at a right angle, and the vertical elastic member (2) is disposed at an inner top corner of the growth plate positioning member (5).

10. Automated pipetting station comprising a culture plate, characterized in that it further comprises a culture plate support device according to any one of claims 1 to 9, the culture plate resting on the vertical spring (2) of the culture plate support device.