CN214716792U

CN214716792U - Fixed microscope carrier of unicellular nucleic acid processing apparatus

Info

Publication number: CN214716792U
Application number: CN202120499604.0U
Authority: CN
Inventors: 张四福; 仇春松; 李牧; 姚业春; 戴尧尧; 曹萌萌
Original assignee: Suzhou New Geyuan Biotechnology Co ltd
Current assignee: Suzhou New Geyuan Biotechnology Co ltd
Priority date: 2021-03-09
Filing date: 2021-03-09
Publication date: 2021-11-16
Anticipated expiration: 2031-03-09

Abstract

The utility model relates to a unicellular nucleic acid processing apparatus technical field discloses a fixed microscope carrier of unicellular nucleic acid processing apparatus, include: the connecting seat is provided with an installation groove; the platform body is placed in the mounting groove, and a placing groove, a first placing hole, a second placing hole, a sample hole and a waste liquid hole are formed in the platform body; the eccentric limit knob is rotatably arranged on the connecting seat and can be abutted against the carrier body so that the carrier body is positioned in the mounting groove, and the eccentric limit knob can also be separated from the carrier body. The utility model discloses a unicellular nucleic acid processing apparatus's fixed microscope carrier is integrated on unicellular nucleic acid processing apparatus, and eccentric limit knob can fix a position microscope carrier body in the mounting groove, and disposable straw, reagent pipe, sample tube and waste liquid pipe all place on microscope carrier body, and microscope carrier body can not empty, has increased the probability that the experiment was successful.

Description

Fixed microscope carrier of unicellular nucleic acid processing apparatus

Technical Field

The utility model relates to a unicellular nucleic acid processing apparatus technical field especially relates to a fixed microscope carrier of unicellular nucleic acid processing apparatus.

Background

When using present unicellular nucleic acid processing apparatus to draw DNA or RNA in the unicellular, the chip is directly placed on the microscope carrier, the chip removes for unicellular nucleic acid processing apparatus very easily, be unfavorable for to dripping into reagent or taking out waste liquid from the chip to the chip in, the probability that the experiment was successful has been reduced, the equal exclusive use rack of disposable straw that is used for holding the reagent pipe of reagent and is used for absorbing the reagent in addition places, in the experimentation, the test-tube rack is emptyd easily, thereby influence the normal clear of experiment.

SUMMERY OF THE UTILITY MODEL

Based on above, the utility model aims to provide a unicellular nucleic acid processing apparatus's fixed microscope carrier has solved the problem that the chip removed easily and the test-tube rack emptys easily, has increased the successful probability of experiment.

In order to achieve the purpose, the utility model adopts the following technical proposal:

a stationary stage of a single-cell nucleic acid processing instrument, comprising: the connecting seat is provided with an installation groove; the microscope carrier comprises a carrier body, wherein the carrier body is placed in the mounting groove, the carrier body is provided with a placing groove, a first placing hole, a second placing hole, a sample hole and a waste liquid hole, the placing groove is used for placing a chip, the first placing hole is used for placing a disposable straw, the second placing hole is used for placing a reagent tube, the sample hole is used for placing a sample tube, and the waste liquid hole is used for placing a waste liquid tube; the eccentric limit knob is rotatably arranged on the connecting seat and can abut against the carrier body so that the carrier body is positioned in the mounting groove, and the eccentric limit knob can be separated from the carrier body.

As a preferable scheme of the fixed carrier of the single cell nucleic acid processing instrument, a distance from a rotating shaft of the eccentric limit knob to an outer periphery of the eccentric limit knob along a radial direction is between a first distance and a second distance, and the distance from the rotating shaft to the carrier body is greater than the first distance and smaller than the second distance.

As a preferred scheme of the fixed carrying platform of the single cell nucleic acid processing instrument, one end of the installation groove is provided with an elastic positioning piece, the length of the elastic positioning piece can change along the length direction of the installation groove, and the elastic positioning piece can be abutted against the chip to limit the chip along the length direction of the chip.

As a preferable embodiment of the fixed stage of the single cell nucleic acid processing apparatus, the elastic positioning member includes a first elastic member and an abutting member, the first elastic member extends along a length direction of the chip, one end of the first elastic member is connected to the stage body, the other end of the first elastic member is connected to the abutting member, and the abutting member can abut against the chip.

As a preferred scheme of the fixed carrier of the single-cell nucleic acid processing instrument, a limiting boss is arranged on the carrier body, the limiting boss and the elastic positioning piece are respectively positioned at two opposite ends of the mounting groove along the length direction of the carrier body, and at least part of the limiting boss is positioned right above the mounting groove so as to limit the chip along the height direction of the chip.

As a preferred scheme of the fixed carrier of the unicellular nucleic acid processing instrument, a taking and placing groove communicated with the placing groove is arranged on the carrier body, and the taking and placing groove is positioned at the corner position of the placing groove.

As a preferred scheme of a fixed carrier of the unicellular nucleic acid processing instrument, one of the connecting seat and the carrier body is provided with a guide piece, and the other is provided with a guide hole matched with the guide piece.

As a preferred scheme of a fixed carrier of a single-cell nucleic acid processing instrument, an elastic limiting component is arranged on the connecting seat, the elastic limiting component is positioned at the end part of the connecting seat, the elastic limiting component comprises an elastic sheet, and the elastic sheet can abut against the carrier body so as to position the carrier body in the mounting groove.

As a preferred scheme of a fixed carrying platform of a single-cell nucleic acid processing instrument, the elastic limiting assembly further comprises a locking member and a second elastic member, the locking member penetrates through the elastic sheet, the connecting seat and the second elastic member, the elastic sheet can rotate relative to the connecting seat, the second elastic member is located below the elastic sheet, and the second elastic member is in a compressed state when the elastic sheet abuts against the carrying platform body.

As a preferred scheme of the fixed carrier of the unicellular nucleic acid processing instrument, a positioning boss is arranged on the carrier body, the first placing hole penetrates through the positioning boss, and the positioning boss is used for supporting the reagent tube.

The utility model has the advantages that: the utility model discloses a unicellular nucleic acid processing apparatus's fixed microscope carrier is integrated on unicellular nucleic acid processing apparatus, and eccentric spacing knob can fix a position microscope carrier body in the mounting groove to the chip rigidity that makes the standing groove that is located microscope carrier body has reduced the probability that the chip removed, and disposable straw, reagent pipe, sample tube and waste liquid pipe all place on microscope carrier body, and microscope carrier body can not empty, has increased the successful probability of experiment.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.

FIG. 1 is a schematic view of a fixed stage of a single-cell nucleic acid processing apparatus according to an embodiment of the present invention;

FIG. 2 is a sectional view of a fixed stage of a single-cell nucleic acid processing apparatus according to an embodiment of the present invention;

FIG. 3 is a schematic view of a part of a fixed stage of the single-cell nucleic acid processing apparatus according to an embodiment of the present invention;

fig. 4 is a partial enlarged view of fig. 2 at a.

In the figure:

1. a connecting seat; 10. mounting grooves; 11. a guide member;

2. a stage body; 201. a placement groove; 202. a first placing hole; 203. a second placing hole; 204. a sample hole; 205. a waste liquid hole; 206. a taking and placing groove; 207. an avoidance groove; 21. a limiting boss; 22. positioning the boss; 23. positioning a plate;

3. an eccentric limit knob;

4. an elastic positioning member; 41. a first elastic member; 42. an abutting member;

5. an elastic limit component; 51. a spring plate; 52. a locking member;

100. and (3) a chip.

Detailed Description

In order to make the technical problems, technical solutions and technical effects achieved by the present invention more clear, the embodiments of the present invention will be described in further detail with reference to the accompanying drawings, and obviously, the described embodiments are only some embodiments, not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The present embodiment provides a fixed stage of a single cell nucleic acid processing apparatus, as shown in fig. 1 to 4, the fixed stage includes a connecting seat 1, a stage body 2, and an eccentric limit knob 3, the connecting seat 1 is installed on a moving component of the single cell nucleic acid processing apparatus, the moving component can drive the connecting seat 1 to move, the connecting seat 1 is provided with an installation groove 10, the stage body 2 is placed in the installation groove 10, the stage body 2 is provided with a placing groove 201, a first placing hole 202, a second placing hole 203, a sample hole 204, and a waste liquid hole 205, the placing groove 201 is used for placing a chip 100, the first placing hole 202 is used for placing a disposable pipette, the second placing hole 203 is used for placing a reagent tube, the sample hole 204 is used for placing a sample tube, the waste liquid hole 205 is used for placing a waste liquid tube, the eccentric limit knob 3 is rotatably installed on the connecting seat 1, the eccentric limit knob 3 can abut against the stage body 2 to position the stage body 2 in the installation groove 10, the eccentric limit knob 3 can also be disengaged from the stage body 2.

Specifically, in the present embodiment, the number of mounting grooves 10 is one, the number of first placement holes 202 is eight, the number of second placement holes 203 is nine, the number of sample holes 204 is two, and the number of waste liquid tanks 205 is one, that is, the fixed stage of the present embodiment can accommodate at most one chip 100, eight disposable pipettes, nine reagent tubes, two sample tubes, and one waste liquid tube. Preferably, as shown in fig. 1, stage body 2 of the present embodiment is provided with positioning bosses 22, first placing holes 202 are provided to penetrate positioning bosses 22, and positioning bosses 22 are used to support reagent tubes.

The fixed microscope carrier integration of the unicellular nucleic acid processing instrument that this embodiment provided is on unicellular nucleic acid processing instrument, and eccentric limit knob 3 can be fixed a position microscope carrier body 2 in mounting groove 10 to the chip 100 position that makes to be located in the standing groove 201 of microscope carrier body 2 is fixed, has reduced the probability that chip 100 removed, and disposable straw, reagent pipe, sample tube and waste liquid pipe all place on microscope carrier body 2, and microscope carrier body 2 can not topple over, has increased the probability that the experiment is successful.

Specifically, the eccentric limit knob 3 of this embodiment rotates with the rotating shaft as the rotating shaft, the rotating shaft is located between the first distance and the second distance along the radial distance to the periphery of the eccentric limit knob 3, the distance from the rotating shaft to the stage body 2 is greater than the first distance and smaller than the second distance, and the limit of the eccentric limit knob 3 to the stage body 2 can be realized by rotating the eccentric limit knob 3. When an experiment is started, firstly, the platform carrier body 2 is placed in the installation groove 10, then the eccentric limit knob 3 is rotated, so that the eccentric limit knob 3 is abutted against the platform carrier body 2, and at the moment, the eccentric limit knob 3 plays a role in fixing the platform carrier body 2 on the connecting seat 1; when the experiment is finished, the eccentric limit knob 3 is rotated, so that the eccentric limit knob 3 is not contacted with the carrier body 2, and the carrier body 2 can be taken down from the connecting seat 1 at the moment.

Further, after the stage body 2 is placed in the mounting groove 10 of the connecting seat 1, the stage body 2 is convexly provided on the connecting seat 1, and in order to make the eccentric limit knob 3 abut against the stage body 2, as shown in fig. 1, an avoiding groove 207 corresponding to the eccentric limit knob 3 is provided on the stage body 2, and when the eccentric limit knob 3 abuts against the stage body 2, a part of the lower end surface of the eccentric limit knob 3 is attached to the bottom wall of the avoiding groove 207.

As shown in fig. 1, 3 and 4, an elastic positioning element 4 is disposed at one end of the mounting groove 10 of the present embodiment, the length of the elastic positioning element 4 is changeable along the length direction of the mounting groove 10, and the elastic positioning element 4 can abut against the chip 100 to limit the chip 100 along the length direction thereof. Specifically, as shown in fig. 4, the elastic positioning member 4 includes a first elastic member 41 and an abutting member 42, the first elastic member 41 extends along the longitudinal direction of the chip 100, one end of the first elastic member 41 is connected to the stage body 2, the other end of the first elastic member 41 is connected to the abutting member 42, and the abutting member 42 can abut against the chip 100. As shown in fig. 3 and 4, the stage body 2 of this embodiment is further provided with a positioning plate 23, the positioning plate 23 is located right above the elastic positioning member 4, and the positioning plate 23 is used to prevent the abutment member 42 and the first elastic member 41 from moving upward, so as to ensure that the abutment member 42 can only move along the length direction of the first elastic member 41.

In order to prevent the chip 100 from moving along its own height direction, as shown in fig. 1, a limiting boss 21 is provided on the stage body 2 of this embodiment, the limiting boss 21 and the elastic positioning element 4 are respectively located at two opposite ends of the mounting groove 10 along its own length direction, and at least part of the limiting boss 21 is located directly above the mounting groove 10 to limit the chip 100 along its own height direction. When the chip 100 is mounted, one end of the chip 100 is first mounted on the end of the mounting groove 10 where the limiting boss 21 is disposed, and then the chip 100 is pressed downward, because the elastic positioning member 4 has elasticity, the chip 100 can push the abutting member 42 to compress the first elastic member 41, so that the chip 100 is mounted in the mounting groove 10.

As shown in fig. 1, the stage body 2 of the present embodiment is provided with a pick-and-place slot 206 communicating with the placement slot 201, and the pick-and-place slot 206 is located at a corner position of the placement slot 201. The number of the picking and placing grooves 206 is four, each picking and placing groove 206 is located at one corner of the placing groove 201, the added picking and placing groove 206 is convenient for placing the chip 100 in the placing groove 201, and meanwhile, an operator can take out the chip 100 in the placing groove 201 conveniently. In other embodiments, the number of the pick-and-place slots 206 is not limited to four in this embodiment, and may be two or three, specifically set according to actual needs.

As shown in fig. 1 and 3, the connecting seat 1 of this embodiment is provided with the guide member 11, the stage body 2 is provided with a guide hole (not shown in the figure) that is matched with the guide member 11, and the guide member 11 and the guide hole can play a role in initially positioning the stage body 2 on the connecting seat 1, so as to prevent the stage body 2 from moving in the horizontal direction with respect to the connecting seat 1. Specifically, the guide 11 of this embodiment is the guide pin, and the fixed setting of guide pin is on connecting seat 1 and the number of guide pin is two, and the number that is equipped with guiding hole and guiding hole on microscope carrier body 2 is two, and two guide pins set up with two guiding holes one-to-one. In other embodiments, the number of the guiding members 11 is not limited to two in this embodiment, and may be three or more than three, which is selected according to actual needs. In other embodiments, the carrier body 2 may be provided with a guide 11, and the connecting seat 1 may be provided with a guide hole engaged with the guide 11.

As shown in fig. 1 and 3, the connecting seat 1 of this embodiment is provided with two elastic limiting components 5, the two elastic limiting components 5 are respectively located at two opposite ends of the connecting seat 1, the elastic limiting components 5 include elastic pieces 51, and the elastic pieces 51 can abut against the stage body 2 to position the stage body 2 in the mounting groove 10.

As shown in fig. 1, the elastic limiting assembly 5 of the present embodiment further includes a locking member 52 and a second elastic member, the locking member 52 penetrates through the elastic sheet 51, the connecting seat 1 and the second elastic member, the elastic sheet 51 can rotate relative to the connecting seat 1, the second elastic member is located below the elastic sheet 51, and the second elastic member is in a compressed state when the elastic sheet 51 abuts on the stage body 2.

Specifically, the locking member 52 of the present embodiment is composed of a fastening member and three nuts, wherein one nut is screwed on the lower end of the fastening member, the elastic member abuts against the nut, the other two nuts are screwed on the fastening member and are located between the elastic sheet 51 and the connecting base 1, and the elastic sheet 51 is clamped between the two nuts and the fixed end of the fastening member. When the shell fragment 51 is rotated to needs, upwards pulling fastener rotates the fastener simultaneously, shell fragment 51 and three nut all along with the fastener for connecting seat 1 at the horizontal plane internal rotation this moment, the elastic component is in compression state, there is the clearance shell fragment 51 below nut and connecting seat 51 to make shell fragment 51, fastener and three nut can rotate for connecting seat 51, when shell fragment 51 and microscope carrier body 52 butt, loosen the fastener, the elastic component can promote the nut and drive the fastener and reset.

It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.

Claims

1. A fixed stage of a single-cell nucleic acid processing instrument, comprising:

the connecting device comprises a connecting seat (1), wherein a mounting groove (10) is formed in the connecting seat (1);

the microscope carrier comprises a carrier body (2), wherein the carrier body (2) is placed in the mounting groove (10), a placing groove (201), a first placing hole (202), a second placing hole (203), a sample hole (204) and a waste liquid hole (205) are formed in the carrier body (2), the placing groove (201) is used for placing a chip (100), the first placing hole (202) is used for placing a disposable pipette, the second placing hole (203) is used for placing a reagent tube, the sample hole (204) is used for placing a sample tube, and the waste liquid hole (205) is used for placing a waste liquid tube;

the eccentric limit knob (3) is rotatably arranged on the connecting seat (1), the eccentric limit knob (3) can abut against the carrier body (2) to enable the carrier body (2) to be positioned in the mounting groove (10), and the eccentric limit knob (3) can be separated from the carrier body (2).

2. The fixed stage of the single-cell nucleic acid processing instrument according to claim 1, wherein the distance from the axis of rotation of the eccentric limit knob (3) to the periphery thereof in the radial direction is between a first distance and a second distance, and the distance from the axis of rotation to the stage body (2) is greater than the first distance and smaller than the second distance.

3. The fixed stage of a single-cell nucleic acid processing instrument as claimed in claim 1, wherein an elastic positioning member (4) is disposed at one end of the mounting groove (10), the length of the elastic positioning member (4) is variable along the length direction of the mounting groove (10), and the elastic positioning member (4) can abut against the chip (100) to position the chip (100) along its length direction.

4. The fixed stage of the single-cell nucleic acid processing instrument according to claim 3, wherein the elastic positioning member (4) includes a first elastic member (41) and an abutting member (42), the first elastic member (41) extends in a longitudinal direction of the chip (100), one end of the first elastic member (41) is connected to the stage body (2), the other end of the first elastic member (41) is connected to the abutting member (42), and the abutting member (42) is capable of abutting against the chip (100).

5. The fixed carrier of single-cell nucleic acid processing instrument as claimed in claim 3, wherein the carrier body (2) is provided with a limit boss (21), the limit boss (21) and the elastic positioning element (4) are respectively located at two opposite ends of the mounting groove (10) along the length direction thereof, and at least part of the limit boss (21) is located directly above the mounting groove (10) to limit the chip (100) along the height direction thereof.

6. The fixed stage of a single-cell nucleic acid processing apparatus according to claim 1, wherein the stage body (2) is provided with a pick-and-place slot (206) communicating with the placement slot (201), and the pick-and-place slot (206) is located at a corner position of the placement slot (201).

7. The fixed stage of a single-cell nucleic acid processing instrument as claimed in claim 1, wherein one of the connecting base (1) and the stage body (2) is provided with a guide (11), and the other is provided with a guide hole engaged with the guide (11).

8. The fixed stage of the instrument for processing single-cell nucleic acid according to claim 1, wherein the connecting base (1) is provided with an elastic limiting component (5), the elastic limiting component (5) is located at an end of the connecting base (1), the elastic limiting component (5) comprises a spring plate (51), and the spring plate (51) can abut against the stage body (2) to position the stage body (2) in the mounting groove (10).

9. The fixed stage of a single-cell nucleic acid processing instrument as claimed in claim 8, wherein the elastic limiting assembly (5) further comprises a locking member (52) and a second elastic member, the locking member (52) penetrates through the spring plate (51), the connecting seat (1) and the second elastic member, the spring plate (51) can rotate relative to the connecting seat (1), the second elastic member is located below the spring plate (51), and the second elastic member is in a compressed state when the spring plate (51) abuts against the stage body (2).

10. The fixed stage of a single-cell nucleic acid processing instrument according to claim 1, wherein a positioning boss (22) is provided on the stage body (2), the first placing hole (202) is provided through the positioning boss (22), and the positioning boss (22) is used for supporting the reagent tube.