CN218653071U

CN218653071U - Chlorophyll extraction element

Info

Publication number: CN218653071U
Application number: CN202222877812.7U
Authority: CN
Inventors: 张祖豪; 彭瑞冰; 吴志宏; 刘露瑶; 吴嘉豪; 夏心怡
Original assignee: Ningbo University
Current assignee: Ningbo University
Priority date: 2022-10-31
Filing date: 2022-10-31
Publication date: 2023-03-21
Anticipated expiration: 2032-10-31

Abstract

The utility model provides a chlorophyll extraction element, including the supporting seat, be provided with the filter unit on the supporting seat and grind and draw the unit, the filter unit with grind and draw and be provided with filter paper transfer unit between the unit, filter paper transfer unit rotates with the supporting seat to be connected, filter paper transfer unit is used for carrying filter paper to grinding from the filter unit and draws the unit, compares with prior art, the utility model discloses a filter paper transfer unit realizes that filter paper carries to grinding from the filter unit and draws the unit, can replace the mode of traditional manual transfer filter paper, realizes semi-automatic mode of drawing to avoid filter paper to drop in the transfer process and influence the experimental result, thereby improve the stability of experiment and the reliability of experimental data.

Description

Chlorophyll extraction element

Technical Field

The utility model relates to a chlorophyll technical field particularly, relates to a chlorophyll extraction element.

Background

Microalgae, which generally refers to a microscopic group of algae whose morphology can only be identified under a microscope, is a generic term for microorganisms that contain chlorophyll a and can perform photosynthesis, and is one of protists. The microalgae cells contain vitamin A, vitamin E, thiamine, riboflavin, pyridoxine, vitamin B12, vitamin C, biotin, inositol, folic acid, calcium pantothenate, nicotinic acid, etc., which increase their value as single-cell proteins. The pigment contained in microalgae has coloring and nourishing effects, and can be used for preventing and treating cancer, resisting radiation, delaying aging, and enhancing immunity.

However, in the laboratory, the procedure of the extraction method of microalgae chlorophyll is complicated, and microalgae cells need to be enriched on a filter paper by suction filtration, and then the filter paper is manually lifted and transferred into a grinding bowl, as disclosed in the published patent No. US20120144903A1, which discloses a method of extracting chlorophyll and a method of measuring the consistency of chlorophyll, which is transferred by tweezers during the transfer process. However, in the transfer process of the laboratory, when the filter paper is transferred by using tweezers or other fixtures, the filter paper often falls off, so that the microalgae cells are polluted, the experimental result is affected, and the stability of the chlorophyll extraction experiment and the reliability of the experimental data cannot be ensured.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the problem that will solve is in chlorophyll extraction experiment, how to avoid filter paper to drop to and how to improve the stability of experiment and the reliability of experimental data.

In order to solve the problem, the utility model provides a chlorophyll extraction element, which comprises a supporting seat, be provided with the filter unit on the supporting seat and grind and draw the unit, the filter unit with it draws to grind to be provided with filter paper between the unit and transports the unit, filter paper transport the unit with the supporting seat rotates to be connected, filter paper transports the unit and is used for carrying filter paper to grinding from the filter unit and draws the unit.

Compared with the prior art, the utility model discloses a filter paper transfer unit realizes that filter paper carries to grinding from filter unit and draws the unit, can replace the mode of traditional manual transfer filter paper, realizes semi-automatic mode of drawing to avoid filter paper to drop in the transfer process and influence the experimental result, thereby improve the stability of experiment and the reliability of experimental data.

Preferably, the supporting seat is fixedly provided with a supporting rod, the filter paper transferring unit comprises a connecting plate, the side face of the connecting plate is fixedly provided with at least one hollow filter paper ring for placing filter paper, and the connecting plate is rotatably connected with the supporting rod. The filter paper may be placed on a filter paper ring and the transport of the filter paper from the filter unit to the abrasive extraction unit is achieved by rotating the filter paper ring.

Preferably, the grinding and extracting unit comprises a grinding machine fixedly connected with the supporting seat, a grinding cavity with an upper end opening is arranged in the grinding machine, a collecting bottle for collecting chlorophyll is arranged at the bottom of the grinding machine, and a liquid inlet of the collecting bottle is communicated with the lower end of the grinding cavity. The grinding machine is used for replacing the manual grinding of the traditional mortar in the grinding and extracting steps, so that the problems of insufficient grinding and incomplete extraction frequently occurring in the manual grinding and extracting method can be avoided, and the extraction efficiency is greatly improved.

Preferably, a push rod for poking the filter paper into the grinding cavity is arranged above the grinding machine, and a power transmission unit is arranged between the push rod and the filter paper transferring unit. The power transmission unit can carry out the linkage with push rod and filter paper transfer unit, when filter paper was located to grind between the extraction element, can stab into the grinding intracavity with filter paper through the push rod to carry out next step and grind the step.

Preferably, the power transmission unit includes drive gear, cover on the bracing piece and rotate the driving gear of being connected with the bracing piece, the driving gear with connecting plate fixed connection makes the driving gear can along with the connecting plate rotates in step, drive gear with the bracing piece rotates to be connected, drive gear's terminal surface with the terminal surface looks of driving gear is perpendicular just drive gear with the driving gear meshes mutually, drive gear with be provided with the actuating lever between the push rod, the one end of actuating lever with drive gear fixed connection, the other end of actuating lever with the push rod is fixed mutually, works as the driving gear along with when the connecting plate rotates, can drive reciprocating motion about the push rod. Through the cooperation of driving gear and drive gear and actuating lever, can realize, when filter paper conveying ground the extraction element, can be synchronous with the downstream of push rod to stab into grinding chamber with filter paper.

Preferably, the driving rod comprises a first connecting rod, a second connecting rod and a third connecting rod, the first connecting rod is horizontally arranged on the third connecting rod, the second connecting rod is perpendicular to the first connecting rod or the third connecting rod, one end of the first connecting rod is fixedly connected with the end face of the driving gear, the other end of the first connecting rod is rotatably connected with one end of the second connecting rod, the other end of the second connecting rod is rotatably connected with one end of the third connecting rod, the other end of the third connecting rod is sleeved on the push rod and is fixed with the push rod, a guide sleeve is fixedly arranged on the supporting rod, and the push rod penetrates through the guide sleeve to enable the push rod to move up and down relative to the guide sleeve.

Preferably, the filter paper transfer unit comprises three hollow filter paper rings which are arranged at intervals on the circumference and used for placing filter paper, and the transmission ratio of the driving gear to the driving gear is 3:1. The filter paper rings arranged at intervals on the three circumferences are adopted, the filter membrane passes through the filter paper rings and sequentially passes through the steps of fixing, suction filtration and grinding to extract, and the three filter paper rings of the filter paper transfer unit are designed to enable the device to simultaneously perform the three steps, so that the chlorophyll extraction speed of the microalgae is improved.

Preferably, the filter unit includes sample bottle and buchner flask that from the top down set gradually, the sample bottle with the bracing piece is fixed mutually, the buchner flask is placed on the supporting seat, the bottom of sample bottle is provided with the liquid outlet, just the upper end of buchner flask is provided with the inlet, the liquid outlet with the inlet is located same vertical direction, the bottom of sample bottle with be formed with the clearance that supplies the filter paper ring to pass through between the upper end of buchner flask. The filter paper ring is arranged in the gap for the filter paper ring to pass through, so that the function of quick suction filtration can be realized only by vacuumizing during filtration.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic structural diagram of the filter paper transfer unit of the present invention.

Description of reference numerals:

the filter paper transferring device comprises a supporting seat 1, a filtering unit 2, a grinding and extracting unit 3, a filter paper transferring unit 4, a power transmission unit 5, a supporting rod 11, a guide sleeve 12, a sample bottle 21, a suction filtration bottle 22, a grinding machine 31, a collection bottle 32, a push rod 6, a connecting plate 41, a filter paper ring 42, a driving gear 51, a driving gear 52, a driving rod 53, a first connecting rod 531, a second connecting rod 532 and a third connecting rod 533.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, embodiments accompanied with figures are described in detail below.

In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "front", "rear", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

The following are specific embodiments of the present invention and the accompanying drawings are used to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

It should be noted that all directional indicators (such as upper, lower, left, right, front, and rear … …) in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.

As shown in fig. 1, a chlorophyll extraction element, including supporting seat 1, be provided with filter unit 2 and grind on the supporting seat 1 and draw unit 3, filter unit 2 with grind and be provided with filter paper transfer unit 4 between drawing unit 3, filter paper transfer unit 4 rotates with supporting seat 1 to be connected, filter paper transfer unit 4 is used for carrying filter paper to grind from filter unit 2 and draws unit 3. In prior art, need artifically to take filter paper from filter unit 2 to grind extraction element 3, hand or tweezers are at the in-process of mentioning filter paper, and the chlorophyll extract on the filter paper is polluted easily, and this device can replace the mode of traditional manual transfer filter paper through being provided with filter paper transfer unit 4 to avoid filter paper to drop in the transfer process and influence the experimental result, thereby improve the stability of experiment and the reliability of experimental data.

In this embodiment, the supporting seat 1 is fixedly provided with the supporting rod 11, the filter paper transferring unit 4 includes the connecting plate 41, the side of the connecting plate 41 is fixedly provided with at least one hollow filter paper ring 42 for placing filter paper, the connecting plate 41 is rotatably connected with the supporting rod 11, the filter paper ring 42 can be placed on the filter paper ring 42 by the structure of the filter paper ring 42, and the filter paper can be conveyed from the filtering unit 2 to the grinding and extracting unit 3 by rotating the filter paper ring 42.

Further, grind and draw unit 3 including grinding machine 31, grind machine 31 and supporting seat 1 fixed connection, be provided with the grinding chamber that has the upper end open-ended in grinding machine 31, the bottom of grinding machine 31 is provided with the receiving flask 32 that is used for collecting the chlorophyll, the income liquid mouth of receiving flask 32 is linked together with the lower extreme of grinding the chamber, adopts and has used the artifical grinding of grinding machine replacement traditional mortar in grinding the extraction step, can avoid the artifical grinding extraction method often to appear grinding insufficient, draw incomplete problem, promote extraction efficiency by a wide margin.

Further, a push rod 6 used for poking the filter paper into the grinding cavity is arranged above the grinding machine 31, a power transmission unit 5 is arranged between the push rod 6 and the filter paper transfer unit 4, the power transmission unit 5 comprises a driving gear 51, a driving gear 52 sleeved on the support rod 11 and rotatably connected with the support rod 11, the driving gear 52 is fixedly connected with the connection plate 41, the driving gear 52 can synchronously rotate along with the connection plate 41, the driving gear 51 is rotatably connected with the support rod 11, the end face of the driving gear 51 is perpendicular to the end face of the driving gear 52, the driving gear 51 is meshed with the driving gear 52, a driving rod 53 is arranged between the driving gear 51 and the push rod 6, one end of the driving rod 53 is fixedly connected with the driving gear 51, the other end of the driving rod 53 is fixed with the push rod 6, and when the driving gear 52 rotates along with the connection plate 41, the push rod 6 can be driven to reciprocate up and down.

In this embodiment, the driving rod 53 includes a first connecting rod 531, a second connecting rod 532, and a third connecting rod 533, the first connecting rod 531 and the third connecting rod 533 are horizontally disposed, the second connecting rod 532 is perpendicular to the first connecting rod 531 or the third connecting rod 533, one end of the first connecting rod 531 is fixedly connected to an end surface of the driving gear 51, the other end of the first connecting rod 531 is rotatably connected to one end of the second connecting rod 532, the other end of the second connecting rod 532 is rotatably connected to one end of the third connecting rod 533, the other end of the third connecting rod 533 is sleeved on the push rod 6 and is fixed to the push rod 6, the support rod 11 is fixedly provided with the guide sleeve 12, and the push rod 6 passes through the guide sleeve 12 so that the push rod 6 can reciprocate up and down relative to the guide sleeve 12.

Referring to fig. 2, the filter paper transfer unit 4 includes three hollow filter paper rings 42 arranged at intervals on the circumference for placing filter paper, and the transmission ratio of the driving gear 52 to the driving gear 51 is 3:1. The filter paper ring that adopts three circumference interval arrangement to set up, its filter membrane leads to the filter paper ring and loops through fixed, suction filtration and grinding and draws the step, and the three filter paper ring design of filter paper transfer unit makes this device can carry out above three steps simultaneously, improves little algae's chlorophyll extraction rate.

In this embodiment, filter unit 2 includes sample bottle 21 and buchner flask 22 that from the top down set gradually, sample bottle 21 is fixed mutually with bracing piece 11, buchner flask 22 is placed on supporting seat 1, the bottom of sample bottle 21 is provided with the liquid outlet, buchner flask 22's upper end is provided with the inlet, the liquid outlet is located same vertical direction with the inlet, be formed with the clearance that supplies filter paper ring 42 to pass through between sample bottle 21's bottom and buchner flask 22's the upper end, the clearance that can supply the filter paper ring to pass through is set up, can be when filtering, only need carry out the function that the evacuation just realized quick suction filtration.

With reference to fig. 1 and 2, the utility model is used as follows: firstly, filter paper is placed on a filter paper ring 42 in a filter paper transfer unit 4, then the filter paper transfer unit 4 is rotated, the filter paper ring 42 with the filter paper is rotated to a position between a sample bottle 21 and a filter bottle 22, then algae liquid is poured into the sample bottle 21, the filter bottle 22 is connected with an external vacuum pump, the algae liquid in the sample bottle 21 is subjected to suction filtration, microalgae cells can be enriched on the filter paper, after the suction filtration is finished, the filter paper transfer unit 4 is continuously rotated, the filter paper ring 42 with the filter paper is rotated to a grinding cavity, in the rotating process of a connecting plate 41, a driving gear 52 rotates along with the connecting plate 41, a push rod 6 moves downwards through the cooperation of a driving gear 51, a first connecting rod 531, a second connecting rod 532 and a third connecting rod 533, so that the filter paper which is just rotated to the grinding cavity is poked into the grinding cavity, then an organic solvent is added into the grinding cavity, a switch of the grinding machine 31 is opened, the filter paper is crushed and ground through a cutter blade in the grinding machine 31, chlorophyll is extracted, and finally flows into a microalgae collecting bottle 32, so that chlorophyll is extracted from the grinding machine.

More preferred embodiments are: when filter paper is placed on the filter paper ring 42 for the first time, the filter paper transfer unit 4 is rotated, and when the filter paper ring 42 with the filter paper is rotated to a position between the sample bottle 21 and the filter flask 22, new filter paper can be continuously added into the blank filter paper ring 42, so that the operations of suction filtration and extraction can be simultaneously carried out, and the chlorophyll extraction speed of the microalgae is improved.

Although the present disclosure has been described above, the scope of the present disclosure is not limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present disclosure, and such changes and modifications will fall within the scope of the present invention.

Claims

1. The utility model provides a chlorophyll extraction element, its characterized in that, includes supporting seat (1), be provided with filter unit (2) and grinding extraction element (3) on supporting seat (1), filter unit (2) with it transports unit (4) to grind to be provided with filter paper between extraction element (3), filter paper transport unit (4) with supporting seat (1) rotates and is connected, filter paper transport unit (4) are used for carrying filter paper to grinding extraction element (3) from filter unit (2).

2. The chlorophyll extraction apparatus according to claim 1, wherein a support rod (11) is fixedly disposed on the support base (1), the filter paper transfer unit (4) comprises a connection plate (41), at least one hollow filter paper ring (42) for placing filter paper is fixedly disposed on a side surface of the connection plate (41), and the connection plate (41) is sleeved on the support rod (11) and is rotatably connected with the support rod (11).

3. A chlorophyll extraction apparatus according to claim 2, wherein the milling extraction unit (3) comprises a milling machine (31), the milling machine (31) is fixedly connected with the support base (1), a milling chamber with an upper end opening is provided in the milling machine (31), a collection bottle (32) for collecting chlorophyll is provided at the bottom of the milling machine (31), and a liquid inlet of the collection bottle (32) is communicated with a lower end of the milling chamber.

4. A chlorophyll extraction apparatus according to claim 3, wherein a push rod (6) for thrusting the filter paper into the grinding chamber is provided above the grinding machine (31), and a power transmission unit (5) is provided between the push rod (6) and the filter paper transfer unit (4).

5. A chlorophyll extraction apparatus according to claim 4, wherein said power transmission unit (5) comprises a driving gear (51) and a driving gear (52) fitted around the support rod (11) and rotatably connected to the support rod (11), said driving gear (52) being fixedly connected to said connecting plate (41) such that said driving gear (52) can rotate synchronously with said connecting plate (41), said driving gear (51) being rotatably connected to said support rod (11), an end surface of said driving gear (51) being perpendicular to an end surface of said driving gear (52) and said driving gear (51) being engaged with said driving gear (52), a driving rod (53) being disposed between said driving gear (51) and said push rod (6), one end of said driving rod (53) being fixedly connected to said driving gear (51), the other end of said driving rod (53) being fixed to said push rod (6), and said driving rod (6) being driven to reciprocate up and down when said driving gear (52) rotates with said connecting plate (41).

6. The chlorophyll extraction apparatus according to claim 5, wherein the driving lever (53) includes a first link (531), a second link (532) and a third link (533), the first link (531) and the third link (533) are horizontally disposed, the second link (532) is perpendicular to the first link (531) or the third link (533), one end of the first link (531) is fixedly connected to an end surface of the driving gear (51), the other end of the first link (531) is rotatably connected to one end of the second link (532), the other end of the second link (532) is rotatably connected to one end of the third link (533), the other end of the third link (533) is fitted over the push rod (6) and fixed to the push rod (6), a guide sleeve (12) is fixedly disposed on the support rod (11), and the push rod (6) passes through the guide sleeve (12) so that the push rod (6) can reciprocate up and down with respect to the guide sleeve (12).

7. A chlorophyll extraction apparatus according to claim 5, wherein said filter paper transfer unit (4) comprises three hollow filter paper rings (42) arranged circumferentially at intervals for placing filter paper thereon, and a transmission ratio of said driving gear (52) to said driving gear (51) is 3:1.

8. A chlorophyll extraction apparatus according to any one of claims 2 to 7, wherein the filtration unit (2) comprises a sample bottle (21) and a filter flask (22) arranged in sequence from top to bottom, the sample bottle (21) is fixed to the support rod (11), the filter flask (22) is placed on the support base (1), a liquid outlet is arranged at the bottom of the sample bottle (21), a liquid inlet is arranged at the upper end of the filter flask (22), the liquid outlet and the liquid inlet are located in the same vertical direction, and a gap for a filter paper ring (42) to pass through is formed between the bottom of the sample bottle (21) and the upper end of the filter flask (22).