CN218637408U

CN218637408U - Aseptic centrifuging tube

Info

Publication number: CN218637408U
Application number: CN202222792003.6U
Authority: CN
Inventors: 王国强; 焦洪权; 曹冉; 张童童; 刘佳
Original assignee: Beijing Chengda Tianhe Biotechnology Co ltd
Current assignee: Beijing Chengda Tianhe Biotechnology Co ltd
Priority date: 2022-10-21
Filing date: 2022-10-21
Publication date: 2023-03-17
Anticipated expiration: 2032-10-21

Abstract

The utility model belongs to the technical field of the experimental device technique and specifically relates to a aseptic centrifuging tube is related to, including the centrifuging tube body, the centrifuging tube body has the sample chamber that holds sample liquid and holds the balancing chamber of balancing liquid, and sample chamber and balancing chamber are mutually independent, the centrifuging tube body has the first liquid mouth that communicates in sample chamber and communicates in the second liquid mouth of balancing chamber, the centrifuging tube body is provided with first shutoff piece and the second shutoff piece to first liquid mouth and the shutoff of second liquid mouth respectively. This application has under the condition of being equipped with the sample, can also carry out effective balancing when keeping the sample aseptic to improve centrifuge bowl's availability's effect.

Description

Aseptic centrifuging tube

Technical Field

The application relates to the technical field of experimental equipment, in particular to an aseptic centrifuge tube.

Background

The centrifugal tube is one of the more instruments used in the experiment, and the application field is wider, especially biological engineering.

In the biological experiment process, a target product in a sterile sample such as cell sap or bacterial liquid and the like is often collected by a centrifugation method, and in order to ensure that the sterile target product is obtained, the sample is required to be ensured not to be polluted in the whole centrifugation process. During centrifugation of a sample, sterile samples are typically dispensed into sterile centrifuge tubes in a biosafety cabinet or clean bench. Although the samples dispensed by each centrifugal tube are equal, the weights of the centrifugal tubes still have certain difference, and the centrifugal operation is directly performed, so that the gravity center and the rotation center of the centrifugal machine are easily deviated, the centrifugal machine is damaged, and the sample centrifugal tubes are required to be strictly balanced.

With respect to the related art among the above, the inventors consider that the following drawbacks exist: because need continuously ventilate in the biosafety cabinet or the superclean bench, be difficult to accomplish the balancing work between aseptic sample centrifuging tube inside, and if the centrifuging tube that is equipped with aseptic sample carried out the balancing under open environment, then need open the sealed lid of centrifuging tube in order to carry out the liquid and supply in order carrying out the balancing, so, can cause the pollution to the aseptic sample that has filled in the centrifuging tube. Therefore, in the existing balancing method, a new centrifugal tube (i.e., a non-specimen centrifugal tube) and a specimen centrifugal tube are balanced, and then the new centrifugal tube and the specimen centrifugal tube are placed into a centrifugal groove of a centrifuge together for operation, the new centrifugal tube does not contain a specimen but occupies the centrifugal groove, the amount of the specimen which can be centrifuged by the centrifuge is reduced, the effective utilization rate of the centrifugal groove is low, and the experimental efficiency is reduced, so that further improvement is needed.

SUMMERY OF THE UTILITY MODEL

In order to solve how under the circumstances of being equipped with the sample, can also carry out effective balancing when keeping the sample aseptic to improve centrifuge bowl's availability, this application provides a aseptic centrifuging tube.

The application provides a pair of aseptic centrifuging tube adopts following technical scheme:

the utility model provides an aseptic centrifuging tube, includes the centrifuging tube body, the centrifuging tube body has the sample chamber that holds sample liquid and holds the balancing chamber of balancing liquid, and sample chamber and balancing chamber are mutually independent, the centrifuging tube body has and communicates in the first liquid mouth of sample chamber and communicates in the second liquid mouth of balancing chamber, the centrifuging tube body is provided with first shutoff piece and the second shutoff piece to first liquid mouth and the shutoff of second liquid mouth respectively.

Through adopting above-mentioned technical scheme, set up sample chamber and balancing chamber on the centrifuging tube body, just so can also carry out the sample assembly in sample chamber at first in aseptic environment, directly carry out the balancing operation to the sample tube that is equipped with the sample under open environment again, because two cavities independent settings do not communicate with each other, so can not cause the pollution because of the balancing operation to the sample, guaranteed the aseptic of sample. A centrifuging tube has had the function of sample assembly and balancing operation concurrently, need not to use new centrifuging tube to carry out the balancing, improves the effective utilization ratio in centrifugal groove, improves experiment work efficiency then.

Preferably, the centrifuging tube body includes the sample cell, and the inner chamber of sample cell does the sample chamber, the one end of sample cell is the blind end, and the other end of sample cell forms for the open end first liquid mouth, first closing cap include first closing cap, and first closing cap can be dismantled and connect in the open end of sample cell in order to carry out the shutoff to first liquid mouth, and the one end that the sample cell was kept away from to first closing cap has the portion of leveling of joining in marriage, and the portion of leveling of joining in marriage is all set up in the portion of leveling of chamber, second liquid mouth, and the second closing cap can be dismantled and connect in the portion of leveling of joining in marriage.

By adopting the technical scheme, the sample tube is used for storing sample liquid, the first liquid port is blocked by the first sealing cover, the balancing part is arranged on the sample tube, the balancing liquid is filled in the balancing tube through the second liquid port, the second liquid port is blocked by the second blocking piece after the balancing liquid in the balancing cavity is increased, decreased and balanced, and the operation is convenient and the practicability is strong.

Preferably, the first closing cap is in threaded connection with the open end of the sample tube.

Through adopting above-mentioned technical scheme, realize dismantling of first closing cap and sample cell through threaded connection mode and be connected, improve the connection stability of first closing cap and sample cell.

Preferably, the peripheral wall of the first sealing cover is provided with first anti-slip threads.

Through adopting above-mentioned technical scheme, through setting up first anti-skidding line, increase the roughness of first closing cap periphery wall, convenient to use person rotates first closing cap.

Preferably, the second blocking member comprises a second cover, and the second cover is connected with the flat part in a threaded mode.

Through adopting above-mentioned technical scheme, realize the dismantlement of second closing cap and balancing portion through threaded connection mode and be connected, improve the connection stability of second closing cap and balancing portion.

Preferably, the peripheral wall of the second cover is provided with second anti-slip threads.

Through adopting above-mentioned technical scheme, through setting up second anti-skidding line, increase the roughness of second closing cap periphery wall, convenient to use person rotates the second closing cap.

Preferably, the second liquid port is opened in the one end that the first closing cap was kept away from in the balance portion, the coaxial fixed cover of internal perisporium that first closing cap one end was kept away from in the balance portion is equipped with the spacing ring, second shutoff piece includes the blanking cap, and the plug-in post that the blanking cap protrusion fixedly connected with inserted and located the spacing ring hole, the plug-in post protrusion fixedly connected with have a spacing head, and the spacing head external diameter is greater than the internal diameter of spacing ring, and the spacing head has elasticity.

Through adopting above-mentioned technical scheme, when carrying out the shutoff with the second liquid mouth, only need to apply towards being close to the trim orientation effort to the blanking cover, force the spacing head to take place elastic deformation, when the hole of locating the spacing ring is inserted completely to the spacing head, spacing head deformation resets, the terminal surface butt of the nearly inserted column of spacing head rest in the spacing ring terminal surface, when needs are dismantled the blanking cover, apply towards keeping away from trim orientation effort to the blanking cover for spacing head breaks away from the spacing ring can.

Preferably, the lower end face of the limiting head is provided with a deformation groove extending to the plug column.

Through adopting above-mentioned technical scheme, be convenient for spacing hair to take place elastic deformation to the user installs or dismantles the blanking cover.

Preferably, the second shutoff piece still includes the connecting strip, and the connecting strip is flexible setting, and the one end fixed connection of connecting strip is in the blanking cover, and the other end fixed connection of connecting strip is in the portion of balancing.

Through adopting above-mentioned technical scheme, be provided with the connecting strip, the possibility that the blanking cover takes place to lose when reducing blanking cover and balancing pipe and breaking away from.

Preferably, the centrifuging tube body includes the body, and the both ends of body all are the opening setting in order to form first liquid mouth and second liquid mouth, and the internal perisporium of body is provided with the division board, and the division board separates the inner chamber of body and forms sample chamber and balancing chamber, and first shutoff piece and second shutoff piece are located the both ends of body respectively.

Through adopting the above-mentioned technical scheme, load aseptic sample in the sample intracavity of body back through first liquid mouth, carry out the shutoff through first closing cap to first liquid mouth, before centrifugal operation, the upset body makes the second liquid mouth up, load balancing liquid in the balancing chamber back through the second liquid mouth, through the balancing liquid to the balancing intracavity increase and decrease the balancing back, carry out the shutoff to the second liquid mouth through the second shutoff, place the body that will be equipped with sample and balancing liquid again behind centrifuge's centrifuge bowl, make centrifuge bowl all be equipped with the body, restart centrifuge carries out centrifugal operation, improve centrifuge's utilization ratio, in order to improve work efficiency.

To sum up, the utility model discloses following beneficial effect has:

1. one centrifugal tube has the functions of sample assembly and balancing operation, and a new centrifugal tube is not needed for balancing, so that the effective utilization rate of the centrifugal groove is improved, and the experimental work efficiency is further improved;

2. load aseptic sample in the sample intracavity back of body through first liquid mouth, carry out the shutoff through first shutoff piece to first liquid mouth, before centrifugal operation, the upset body makes the second liquid mouth up, load balancing liquid in the balancing chamber back through the second liquid mouth, through increasing and decreasing the balancing liquid to the balancing intracavity back, carry out the shutoff to the second liquid mouth through the second shutoff piece, place the body that will be equipped with sample and balancing liquid behind centrifuge's centrifugal tank again, improve centrifuge's utilization ratio, in order to improve work efficiency.

Drawings

FIG. 1 is a schematic diagram of a sterile centrifuge tube according to example 1;

FIG. 2 is a schematic view of the structure of a sample tube and a trim tube in example 1;

FIG. 3 is a schematic illustration of the construction of the first and second block piece of example 1;

FIG. 4 is a schematic structural view of the centrifuge tube body in example 2;

FIG. 5 is a schematic view showing the internal structure of the centrifuge tube body in example 2;

FIG. 6 is a schematic diagram of a sterile centrifuge tube according to example 3;

fig. 7 is a schematic structural view of the second plugging member in example 3.

In the figure, 1, centrifuge tube body; 11. a first fluid port; 12. a first external thread; 13. a partition plate; 2. A first blocking piece; 21. a first anti-skid pattern; 22. a first internal thread; 3. leveling a pipe; 31. a second fluid port; 32. a second external thread; 33. a limiting ring; 4. a second blocking member; 41. a second anti-skid pattern; 42. a second internal thread; 43. blocking the cover; 44. a connecting strip; 45. inserting the column; 46. a limiting head; 47. a deformation groove; 48. a force application part.

Detailed Description

The present application is described in further detail below with reference to figures 1-7.

Example 1:

the embodiment of the application discloses aseptic centrifuging tube, referring to fig. 1, including centrifuging tube body 1, centrifuging tube body 1 has the sample chamber that holds sample liquid and holds the balance chamber of balancing liquid. In this embodiment, the centrifuge tube body 1 is a sample tube, an inner cavity of the sample tube is a sample cavity, one end of the sample tube is a closed end, and the other end of the sample tube is an open end to form a first liquid port 11.

Referring to fig. 2 and 3, the sample tube is provided with a first blocking piece 2 for blocking the first liquid port 11, the first blocking piece 2 is a first sealing cover, and the first sealing cover is detachably connected to the open end of the sample tube to block the first liquid port 11. The outer peripheral wall of the opening end of the sample tube is provided with a first external thread 12, the inner peripheral wall of the first sealing cover is provided with a first internal thread 22 in threaded adaptation to the first external thread 12, and the outer peripheral wall of the first sealing cover is provided with a first anti-skid grain 21.

The first sealing cover is provided with a balancing part protruding from one end of the first sealing cover far away from the sample tube, in this embodiment, the balancing part is a balancing tube 3, an inner cavity of the balancing tube 3 is a balancing cavity, one end of the balancing tube 3 is a closed end and is fixedly connected to the outer end face of the first sealing cover, and the other end of the balancing tube 3 is an open end to form a second liquid port 31.

The balance pipe 3 is provided with a second blocking piece 4 for blocking the second liquid port 31, specifically, the second blocking piece 4 is a second sealing cover, the outer peripheral wall of the opening end of the balance pipe 3 is provided with a second external thread 32, the inner peripheral wall of the second sealing cover is provided with a second internal thread 42 in threaded adaptation to the second external thread 32, and the outer peripheral wall of the second sealing cover is provided with a second anti-skid thread 41.

The implementation principle of the embodiment of the application is as follows: filling sterile samples in a sample tube through a first liquid port 11 in a sterile environment, installing a balancing tube 3 in the sample tube through a first sealing cover when plugging the first liquid port 11, before centrifugal operation, filling balancing liquid in an open environment in the balancing tube 3 through a second liquid port 31, increasing and decreasing the balancing liquid in a balancing cavity, plugging the second liquid port 31 through a second sealing cover, and then placing a centrifugal tube body 1 filled with the samples and the balancing liquid in the same centrifugal groove of a centrifugal machine, so that the centrifugal groove is filled with the centrifugal tube body 1, and then the centrifugal machine is started to perform centrifugal operation, thereby improving the utilization rate of the centrifugal machine and improving the working efficiency.

Example 2:

the difference with embodiment 1 lies in, refer to fig. 4, 5, centrifuging tube body 1 includes the body, and the both ends of body all are the opening setting in order to form first liquid mouth 11 and second liquid mouth 31, and the internal perisporium of body is provided with division board 13, and division board 13 separates the inner chamber of body and forms sample chamber and balance chamber. First shutoff piece 2 and second shutoff piece 4 are located the both ends of body respectively in order to carry out the shutoff to first liquid mouth 11 and second liquid mouth 31, and in this embodiment, first shutoff piece 2 and second shutoff piece 4 are sealed spiral cover, and sealed spiral cover adopts the screw thread mode can dismantle the tip of connecting in the body.

The implementation principle of the embodiment of the application is as follows: load aseptic sample in the sample intracavity of body back through first liquid mouth 11 in aseptic environment, carry out the shutoff through first shutoff piece 2 to first liquid mouth 11, before centrifugal operation, the body of overturning makes second liquid mouth 31 up, load balancing liquid in the open environment behind the balancing chamber through second liquid mouth 31, through the balancing liquid to the balancing intracavity increase and decrease balancing back, carry out the shutoff to second liquid mouth 31 through second shutoff piece 4, place the body that is equipped with sample and balancing liquid behind centrifuge's centrifuging slot again, make the centrifuging slot all be equipped with the body, restart centrifuge carries out centrifugal operation, improve centrifuge's utilization ratio, in order to improve work efficiency.

Example 3:

the difference from embodiment 1 is that, referring to fig. 6 and 7, the inner peripheral wall of the opening end of the balance pipe 3 is coaxially and fixedly sleeved with the limiting ring 33, and the second blocking piece 4 includes a blocking cover 43 and a connecting strip 44. The coaxial protrusion fixedly connected with of interior terminal surface of blanking cap 43 inserts the spliced pole 45 of locating the hole of spacing ring 33, and spliced pole 45 protrusion fixedly connected has spacing head 46, and spacing head 46 and spliced pole 45 and blanking cap 43 integrated into one piece just have elasticity for rubber products, and spacing head 46 external diameter is greater than the internal diameter of spacing ring 33, and the lower terminal surface of spacing head 46 is seted up and is extended to the deformation groove 47 of spliced pole 45. The limiting head 46 is arranged in a conical shape, one end of the limiting head 46 close to the plug column 45 is a large end, and one end of the limiting head 46 far away from the plug column 45 is a small end. The periphery wall of the blocking cover 43 is provided with a force application part 48 protruding out of the periphery wall of the balance pipe 3, the connecting strip 44 is flexibly arranged, one end of the connecting strip 44 is fixedly connected to the periphery wall of the blocking cover 43, and the other end of the connecting strip 44 is fixedly connected to the periphery wall of the balance pipe 3.

The implementation principle of the embodiment of the application is as follows: when plugging the second liquid port 31, through applying towards being close to the 3 direction effort of balance pipe to the blanking cover 43, force spacing head 46 to take place elastic deformation, when spacing head 46 inserted the hole of locating spacing ring 33 completely, spacing head 46 deformation resets, spacing head 46 is close to the terminal surface butt in spacing ring 33 terminal surface of plug-in post 45, when needs are dismantled blanking cover 43, apply towards keeping away from 3 direction efforts of balance pipe to force application portion 48, make spacing head 46 break away from out spacing ring 33 can.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. A sterile centrifuge tube, which is characterized in that: the centrifugal tube comprises a centrifugal tube body (1), wherein the centrifugal tube body (1) is provided with a sample cavity for containing sample liquid and a balancing cavity for containing balancing liquid, the sample cavity and the balancing cavity are mutually independent, the centrifugal tube body (1) is provided with a first liquid port (11) communicated with the sample cavity and a second liquid port (31) communicated with the balancing cavity, and the centrifugal tube body (1) is provided with a first blocking piece (2) and a second blocking piece (4) which block the first liquid port (11) and the second liquid port (31) respectively; the centrifuge tube body (1) comprises a sample tube, the inner cavity of the sample tube is the sample cavity, one end of the sample tube is a closed end, the other end of the sample tube is an open end to form the first liquid port (11), the first blocking piece (2) comprises a first sealing cover, the first sealing cover is detachably connected to the open end of the sample tube to block the first liquid port (11), one end, far away from the sample tube, of the first sealing cover is provided with a leveling part, the leveling cavity and the second liquid port (31) are both arranged on the leveling part, and the second blocking piece (4) is detachably connected to the leveling part; second fluid port (31) are seted up in balance portion and are kept away from the one end of first closing cap, balance portion keeps away from the coaxial fixed cover of internal perisporium of first closing cap one end and is equipped with spacing ring (33), second shutoff piece (4) are including blanking cover (43), and blanking cover (43) protrusion fixedly connected with inserts plug-in column (45) of locating spacing ring (33) hole, and plug-in column (45) protrusion fixed connection has spacing head (46), and spacing head (46) external diameter is greater than the internal diameter of spacing ring (33), and spacing head (46) have elasticity.

2. A sterile centrifuge tube according to claim 1 wherein: the first sealing cover is in threaded connection with the opening end of the sample tube.

3. A sterile centrifuge tube according to claim 2, wherein: the peripheral wall of the first sealing cover is provided with first anti-skidding grains (21).

4. A sterile centrifuge tube according to claim 1, wherein: the second blocking piece (4) comprises a second sealing cover which is in threaded connection with the leveling part.

5. A sterile centrifuge tube according to claim 4, wherein: the peripheral wall of the second sealing cover is provided with second anti-slip threads (41).

6. A sterile centrifuge tube according to claim 1, wherein: the lower end face of the limiting head (46) is provided with a deformation groove (47) extending to the insertion column (45).

7. A sterile centrifuge tube according to claim 1 wherein: the second plugging piece (4) further comprises a connecting strip (44), the connecting strip (44) is flexibly arranged, one end of the connecting strip (44) is fixedly connected to the plugging cover (43), and the other end of the connecting strip (44) is fixedly connected to the leveling portion.

8. A sterile centrifuge tube according to claim 1 wherein: centrifuging tube body (1) includes the body, and the both ends of body all are the opening setting in order to form first liquid mouth (11) and second liquid mouth (31), and the internal perisporium of body is provided with division board (13), and division board (13) are separated the inner chamber of body and are formed sample chamber and balancing chamber, and first shutoff piece (2) and second shutoff piece (4) are located the both ends of body respectively.