CN218636496U

CN218636496U - Ultrasonic therapeutic device for in vitro tumor cells

Info

Publication number: CN218636496U
Application number: CN202221984606.XU
Authority: CN
Inventors: 安杰; 贺鑫怡; 马惠珠; 周海涛; 陈耀东; 李亚渊; 武志芳; 李思进
Original assignee: Shanxi Medical University
Current assignee: Shanxi Medical University
Priority date: 2022-07-29
Filing date: 2022-07-29
Publication date: 2023-03-17
Anticipated expiration: 2032-07-29

Abstract

The utility model belongs to the technical field of medical appliances, in particular to an ultrasonic treatment device for in vitro tumor cells, which comprises a fixed frame, a placing plate and a manual moving mechanism, wherein the placing plate is detachably connected with the fixed frame, a step through hole is arranged in the middle of the placing plate, the manual moving mechanism is arranged on the fixed frame and is positioned below the placing plate, and an ultrasonic probe is arranged on the manual moving mechanism; the manual moving mechanism comprises a first sliding rail and a second sliding rail, the first sliding rail is horizontally arranged and is connected with the fixed frame in a sliding manner, and the first sliding rail moves left and right along the fixed frame; the second sliding rail is connected with the first sliding rail in a sliding manner, is vertically arranged and moves back and forth along the first sliding rail; the ultrasonic probe is connected with the second sliding rail in a sliding mode and can move up and down along the second sliding rail. The placing plate is detachably connected with the fixing frame, so that the placing plate is convenient to install, replace and clean; the first sliding rail and the second sliding rail are arranged, so that the movement of the ultrasonic probe in three directions can be realized, and the requirement of actual work is met.

Description

Ultrasonic therapeutic device for in vitro tumor cells

Technical Field

The utility model belongs to the technical field of medical appliances, concretely relates to ultrasonic therapy device of external tumor cell.

Background

At present, surgical treatment, comprehensive treatment, palliative treatment and the like are available for cancer treatment, but the recurrence rate and the death rate are still high. The SDT uses the targeting property that the high concentration of the sonosensitizer is gathered on tumor cells and tumor neovascular endothelial cells, and uses ultrasonic to activate the sonosensitizer after being accurately positioned by imaging under the condition of no wound, so that the sonosensitizer generates ultrasonic chemical reaction to generate singlet oxygen, takes electrons from the tumor cells to destroy organelles of the tumor cells to cause the death of the tumor cells, and destroys epithelial cells of the tumor blood vessels to release thromboxane, and forms thrombus in the blood vessels of the tumor to cause the ischemic necrosis of the tumor tissues, thereby achieving the purpose of accurately and completely killing the tumor without damaging normal cells.

At present, when in-vitro tumor cell sonodynamic therapy is carried out in a laboratory, a cell culture plate and an ultrasonic probe need to be held by an experimenter, so that the operation is very inconvenient. Patent application No. 201910035026.2 discloses an ultrasonic transfection device which works by adopting a mechanical transmission device, but has the defect of complex integral structure; patent application No. 201820512372.6 discloses a device for mediating gene transfection by combining an ultrasonic targeted microbubble destruction technology, which has a simple overall structure but cannot realize multidirectional movement of a probe.

SUMMERY OF THE UTILITY MODEL

To the technical problem, the utility model provides an ultrasonic therapy device of external tumor cell, the device simple structure, and can realize the diversified removal of probe.

In order to solve the technical problem, the utility model discloses a technical scheme be:

an ultrasonic treatment device for in vitro tumor cells comprises a fixed frame, a placing plate and a manual moving mechanism, wherein the placing plate is detachably connected with the fixed frame, a step through hole is formed in the middle of the placing plate, the manual moving mechanism is arranged on the fixed frame and located below the placing plate, and an ultrasonic probe is arranged on the manual moving mechanism; the manual moving mechanism comprises a first sliding rail and a second sliding rail, the first sliding rail is horizontally arranged and is connected with the fixed frame in a sliding manner, and the first sliding rail moves left and right along the fixed frame; the second sliding rail is connected with the first sliding rail in a sliding manner, is vertically arranged and moves back and forth along the first sliding rail; the ultrasonic probe is connected with the second sliding rail in a sliding mode and can move up and down along the second sliding rail.

The first sliding rail is connected with the fixed frame in a sliding manner through a third sliding rail; the third slide rail is equipped with two, and two third slide rails are fixed respectively in the both sides of mount.

The second slide rail is connected with two claw clamps in a sliding manner and is connected with the ultrasonic probe through the two claw clamps; the two-jaw clamp comprises a connecting rod, a first jaw and a second jaw, one end of the first jaw and one end of the second jaw are hinged to the connecting rod, an adjusting bolt is arranged between the first jaw and the second jaw, a spring is sleeved on the adjusting bolt, and two ends of the spring can be abutted to the first jaw and the second jaw.

The fixing frame is provided with a step groove and is connected with the placing plate through the step groove.

Step groove department is equipped with the concave part, can conveniently take out the board of placing through the concave part.

The manual moving mechanism is provided with an ejection mechanism which can extend out of the step through hole.

The ejection mechanism is arranged at one end of the second sliding rail, and the ultrasonic probe is arranged at the other end of the second sliding rail.

The ejection mechanism comprises a connecting plate, a top plate and a handle, the connecting plate is connected with the second sliding rail in a sliding mode, the top plate and the handle are both fixedly connected with the connecting plate, and the top plate can extend out of the stepped through hole.

The top plate is circular in shape.

And a rubber block is fixed at the bottom of the second slide rail.

Compared with the prior art, the utility model, the beneficial effect who has is:

the placing plate is detachably connected with the fixing frame, so that the placing plate can be conveniently installed, replaced and cleaned; the first sliding rail and the second sliding rail are arranged, so that the movement of the ultrasonic probe in three directions can be realized, and the requirement of actual work is met.

The ultrasonic probe can be conveniently fixed through the two jaw clamps; the ejection mechanism is arranged to eject the confocal cuvette, so that the confocal cuvette can be taken out conveniently.

Drawings

Fig. 1 is a schematic view of the split structure of the present invention;

fig. 2 is a front view of the present invention;

fig. 3 is a schematic structural view of the fixing frame of the present invention;

fig. 4 is a schematic structural diagram of the manual moving mechanism of the present invention;

fig. 5 is a schematic structural view of a second slide rail according to the present invention;

fig. 6 is another schematic structural view of the fixing frame of the present invention;

wherein: 1 is the mount, 2 is for placing the board, 3 is manual moving mechanism, 30 is first slide rail, 31 is the second slide rail, 32 is the third slide rail, 33 is the slider, 4 is the ladder through-hole, 5 is two claw presss from both sides, 50 is the connecting rod, 51 is first claw, 52 is the second claw, 53 is adjusting bolt, 54 is the spring, 6 is the ladder recess, 7 is the concave part, 8 is ejection mechanism, 80 is the connecting plate, 81 is the roof, 82 is the handle, 9 is the confocal capsule, 10 is the block rubber.

Detailed Description

The technical solutions in the embodiments of the present invention are described below clearly and completely, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

As shown in fig. 1 to 5, an ultrasonic therapeutic apparatus for in vitro tumor cells comprises a fixing frame 1, a placing plate 2 and a manual moving mechanism 3, wherein the placing plate 2 is detachably connected with the fixing frame 1, a stepped through hole 4 is arranged in the middle of the placing plate 2, a confocal small dish 9 can be placed in the stepped through hole 4, and the bottom of the confocal small dish 9 can be supported at the stepped position of the stepped through hole 4. When the confocal small dish 9 is circular, the stepped through hole 4 is also a circular through hole and can be adjusted and arranged according to actual conditions.

The manual moving mechanism 3 is arranged on the fixed frame 1 and is positioned below the placing plate 2, and an ultrasonic probe is arranged on the manual moving mechanism 3; the manual moving mechanism 3 is mainly operated by a worker to realize the multidirectional movement of the ultrasonic probe.

Preferably: the manual moving mechanism 3 comprises a first slide rail 30 and a second slide rail 31, the first slide rail 30 is horizontally arranged, the first slide rail 30 is connected with the fixed frame 1 in a sliding manner, and the first slide rail 30 moves left and right along the fixed frame 1; the second slide rail 31 is connected with the first slide rail 30 in a sliding manner, the second slide rail 31 is vertically arranged, and the second slide rail 31 moves back and forth along the first slide rail 30; the ultrasonic probe is connected with the second slide rail 31 in a sliding manner, and the ultrasonic probe can move up and down along the second slide rail 31.

Through the structure, the ultrasonic probe can be moved up and down, back and forth and left and right, and the ultrasonic treatment at different positions on the cell culture plate and the confocal cuvette 9 is facilitated.

The sliding connection among the first slide rail 30, the second slide rail 31 and the ultrasonic probe can be realized by adopting various structures; specifically, the method comprises the following steps: the first slide rail 30 and the second slide rail 31 are both connected with a slide block 33 in a sliding manner, the second slide rail 31 is fixedly connected with the slide block 33 connected with the first slide rail 30 in a sliding manner, and the ultrasonic probe is connected with the slide block 33 connected with the second slide rail 31 in a sliding manner.

Further, in order to improve the sliding stability of the first slide rail 30, the first slide rail 30 is slidably connected to the fixing frame 1 through a third slide rail 32; the number of the third slide rails 32 is two, and the two third slide rails 32 are respectively fixed on two sides of the fixing frame 1. Similarly, the third slide rail 32 is also correspondingly connected with a slide block 33 in a sliding manner, and both ends of the first slide rail 30 are fixedly connected with the slide blocks 33 which are connected with the two third slide rails 32 in a sliding manner. That is, the two third slide rails 32 are provided, so that the stability of the first slide rail 30 during sliding can be improved.

Further, the sliding connection of the ultrasonic probe and the second slide rail 31 can be realized by various structures, and the following two-jaw clamp 5 in the prior art is preferably adopted. The specific structure of the two-jaw clamp 5 is well known to those skilled in the art, and therefore, for a brief description, the two-jaw clamp 5 includes a connecting rod 50, a first jaw 51 and a second jaw 52, one end of each of the first jaw 51 and the second jaw 52 is hinged to the connecting rod 50, an adjusting bolt 53 is disposed between the first jaw 51 and the second jaw 52, and a corresponding through hole is disposed on each of the first jaw 51 and the second jaw 52, and the diameter of the through hole is greater than the diameter of the adjusting bolt 53.

A spring 54 is fitted over the adjusting bolt 53, and both ends of the spring 54 can abut against the first and

second pawls

51, 52. The other ends of the first claw 51 and the second claw 52 are both provided with arc-shaped grooves, the handheld part of the ultrasonic probe is placed at the two arc-shaped grooves, and then the adjusting bolt 53 is screwed. Specifically, the method comprises the following steps: the connecting rod 50 is fixedly (in a threaded manner) connected with the sliding block 33 on the second sliding rail 31.

Further, the detachable connection of the placing plate 2 can be realized by various structures, preferably: be equipped with ladder recess 6 (for leading to the groove from top to bottom) on mount 1, be connected with placing board 2 through ladder recess 6, place board 2 promptly and can arrange in this ladder recess 6, the bottom of placing board 2 can offset with the step portion of this ladder recess 6.

Further, ladder groove 6 department is equipped with recessed part 7, and recessed part 7 and ladder groove 6 are in the connected state, can expose the edge of placing board 2 through recessed part 7, and then can conveniently take out placing board 2.

Further, as shown in fig. 6, the stepped groove 6 may be arranged in various structures, such as: the long side part of the stepped groove 6 is not provided with a step part, and the cell culture plate is erected at the stepped groove 6 and is supported by the step part of the short side part of the stepped groove 6. Of course, this structure does not affect the placement of the placement board 2.

When the cell culture plate needs to be subjected to ultrasonic treatment, the placing plate 2 is taken out, and the cell culture plate is placed in the stepped groove 6; when needing to carry out ultrasonic therapy to focus capsule 9, will place board 2 and place ladder recess 6 department in, it can to co-focus capsule 9 on placing board 2.

Further, when the confocal cuvette 9 needs to be removed, the confocal cuvette 9 is not easily removed because the thickness of the confocal cuvette 9 is small and the portion of the confocal cuvette exposed from the placement plate 2 is small after placement. Therefore, the manual moving mechanism 3 is provided with the ejection mechanism 8, and the ejection mechanism 8 can extend out of the stepped through hole 4, so that the confocal small dish 9 is ejected out of the stepped through hole 4, and the taking is convenient.

Further, the ejection mechanism 8 is disposed at one end of the second slide rail 31, and the ultrasonic probe (the two claw clips 5) is disposed at the other end of the second slide rail 31, which are not interfered with each other.

Further, the ejection mechanism 8 comprises a connecting plate 80, a top plate 81 and a handle 82, wherein the connecting plate 80 is slidably connected with the second slide rail 31; two sliding blocks 33 can be arranged on the second sliding rail 31, wherein one sliding block 33 is fixedly connected with the connecting plate 80, and the other sliding block 33 is connected with the ultrasonic probe (the two claw clamps 5).

Roof 81 and handle 82 all with connecting plate 80 fixed connection, roof 81 is located the top of connecting plate 80, and handle 82 is located the below of connecting plate 80, upwards promotes through handheld handle 82, can stretch out roof 81 from ladder through-hole 4 to it is ejecting to locate the confocal boat 9 of placing at ladder through-hole 4.

Further, the top plate 81 is preferably circular in shape.

Further, the rubber block 10 is fixed to the bottom of the second slide rail 31, and the slider 33 slidably connected to the second slide rail 31 can abut against the rubber block 10, thereby restricting the movement of the slider 33. Specifically, the method comprises the following steps: the rubber block 10 is not required to be arranged below the sliding block 33 connected with the ejection mechanism 8, and only a bulge is required to be arranged at the bottom of the second sliding rail 31 to limit the downward movement of the sliding block 33.

The above description has been made in detail only for the preferred embodiment of the present invention, but the present invention is not limited to the above embodiment, and various changes can be made without departing from the spirit of the present invention within the knowledge scope of those skilled in the art, and all such changes are intended to be encompassed by the present invention.

Claims

1. An ultrasonic therapeutic device for in vitro tumor cells, characterized in that: the ultrasonic probe placing device comprises a fixing frame (1), a placing plate (2) and a manual moving mechanism (3), wherein the placing plate (2) is detachably connected with the fixing frame (1), a stepped through hole (4) is formed in the middle of the placing plate (2), the manual moving mechanism (3) is arranged on the fixing frame (1) and located below the placing plate (2), and an ultrasonic probe is arranged on the manual moving mechanism (3); the manual moving mechanism (3) comprises a first sliding rail (30) and a second sliding rail (31), the first sliding rail (30) is horizontally arranged, the first sliding rail (30) is connected with the fixed frame (1) in a sliding mode, and the first sliding rail (30) moves left and right along the fixed frame (1); the second sliding rail (31) is connected with the first sliding rail (30) in a sliding mode, the second sliding rail (31) is vertically arranged, and the second sliding rail (31) moves back and forth along the first sliding rail (30); the ultrasonic probe is connected with the second sliding rail (31) in a sliding mode and can move up and down along the second sliding rail (31).

2. The apparatus for ultrasonic treatment of tumor cells in vitro according to claim 1, wherein: the first sliding rail (30) is connected with the fixed frame (1) in a sliding manner through a third sliding rail (32); the number of the third sliding rails (32) is two, and the two third sliding rails (32) are respectively fixed on two sides of the fixing frame (1).

3. The ultrasonic therapeutic apparatus for in vitro tumor cells according to claim 1, wherein: the second sliding rail (31) is connected with two claw clamps (5) in a sliding manner and is connected with the ultrasonic probe through the two claw clamps (5); the two-jaw clamp (5) comprises a connecting rod (50), a first jaw (51) and a second jaw (52), one end of the first jaw (51) and one end of the second jaw (52) are hinged to the connecting rod (50), an adjusting bolt (53) is arranged between the first jaw (51) and the second jaw (52), a spring (54) is sleeved on the adjusting bolt (53), and two ends of the spring (54) can be abutted to the first jaw (51) and the second jaw (52).

4. The apparatus for ultrasonic treatment of tumor cells in vitro according to claim 1, wherein: the fixing frame (1) is provided with a ladder groove (6) which is connected with the placing plate (2) through the ladder groove (6).

5. The ultrasonic therapeutic apparatus for in vitro tumor cells according to claim 4, wherein: step groove (6) department is equipped with concave part (7), can conveniently take out placing board (2) through concave part (7).

6. The ultrasonic therapeutic apparatus for in vitro tumor cells according to claim 1, wherein: the manual moving mechanism (3) is provided with an ejection mechanism (8), and the ejection mechanism (8) can extend out of the stepped through hole (4).

7. The ultrasonic therapeutic apparatus for in vitro tumor cells according to claim 6, wherein: the ejection mechanism (8) is arranged at one end of the second sliding rail (31), and the ultrasonic probe is arranged at the other end of the second sliding rail (31).

8. The ultrasonic therapeutic apparatus for in vitro tumor cells according to claim 7, wherein: the ejection mechanism (8) comprises a connecting plate (80), a top plate (81) and a handle (82), the connecting plate (80) is in sliding connection with the second sliding rail (31), the top plate (81) and the handle (82) are fixedly connected with the connecting plate (80), and the top plate (81) can extend out of the step through hole (4).

9. The apparatus for ultrasonic treatment of tumor cells in vitro according to claim 8, wherein: the top plate (81) is circular in shape.

10. The ultrasonic therapeutic apparatus for in vitro tumor cells according to claim 1, wherein: and a rubber block (10) is fixed at the bottom of the second sliding rail (31).