CN218618119U - Freeze-stored capsule for storing cell products - Google Patents

Abstract

The utility model relates to a packaging container field that cell goods cryogenically preserved discloses a cryopreserved capsule for cell goods is preserved, and it is including the welding part, both ends or the cylindrical utricule of one end next-door neighbour welding part formation inclined plane that are located capsule both ends or one end, the welding part is for welding the totally enclosed structure who realizes through high frequency heat seal technology or laser welding technology, the anterior utricule that next-door neighbour welding part formed the inclined plane has the double inclined plane that forms 30 degrees ~120 degrees contained angles, cylindrical utricule divides welding reservation portion, welding assistance clamp reservation portion, buffer space portion and sample loading portion behind the filling. The cryopreservation capsule has the advantages of being capable of realizing full sealing and integration, suitable for automatic filling production line operation, capable of reducing the probability of pollution during sample filling, and capable of greatly improving sample filling and exhaust efficiency in the production process.

Description

Freeze-stored capsule for storing cell products

Technical Field

The utility model relates to a packaging container technical field that biological product cryogenically preservation especially relates to a cryopreserved capsule for cell product cryogenically preservation.

Background

The technical field is a packaging container for low-temperature preservation of cell products, which mainly comprises a packaging scheme with structures such as a cell freezing tube, a cell freezing bag, a penicillin bottle and the like.

Although the prior art discloses technical solutions for providing various functions to the cell cryopreservation tube, the cell cryopreservation tube is designed into a split opening and closing structure design of a tube body and a tube cap, and is only suitable for laboratory manual operation and small-batch cell cryopreservation, and cannot be suitable for industrial automatic closed filling and continuous large-scale production.

Although the cell cryopreservation bag used in the prior art, such as the contents disclosed in documents CN208446473U, CN210017600U, CN213095763U, CN213229719U, CN214482994U, US9357763B2 and the like, the scheme disclosed in the prior art solves the problems of being suitable for closed filling and continuous large-scale production to a certain extent, the problems that the structural design and the pipeline design of the packaging container are complex, an independent exhaust component needs to be arranged, the packaging container is soft in material and not easy to fix, and the efficiency of matching with an automatic filling production line is low still exist.

Although the penicillin bottles used in the prior art, such as the content disclosed in documents such as CN100347052C, CN213589077U and the like, have higher rigidity in the structure of packaging containers in the scheme disclosed in the prior art, and the problems of sealability and sample pollution are solved to a certain extent through various component designs, the penicillin bottles are designed in a split structure of bottle bodies and bottle caps, so that the structure for realizing functions is complicated, the operation of an automatic filling production line needing to be adapted is complicated, the risk of sample pollution is increased, and automatic closed filling is difficult to realize.

In addition, after the cell product is filled into the packaging container, in order to prevent the container from cracking due to the violent change of the gas volume in the container in the freezing recovery process, the product is polluted, and the production process needs to carry out degassing treatment before the container is sealed in a heat sealing way. However, the prior packaging container scheme still has the problems of inconvenient exhaust operation, easy gas in the container entering the output assembly, influence on operation efficiency and exhaust effect and the like.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems in the field of packaging containers for preserving cell products at low temperature, a scheme of the packaging container which is fully sealed and integrated, is suitable for the operation of an automatic filling production line, can reduce the pollution probability during sample filling, and can greatly improve the sample filling and exhaust efficiency in the production process is provided.

The utility model provides a cryopreserved capsule for cell product is preserved, include: welding parts at two ends or one end of the capsule, a front capsule body with two ends or one end adjacent to the welding parts to form an inclined plane, and a cylindrical capsule body at the middle or one end.

In some embodiments, the welding part is a fully closed structure that is welded by a high frequency heat sealing process or a laser welding process.

In some embodiments, the anterior balloon forming a chamfer proximate to the weld has a double chamfer forming an included angle of 30-120 degrees; in some embodiments, the anterior balloon forming a chamfer proximate to the weld has a double chamfer forming an included angle of 50-90 degrees; in some embodiments, the anterior bladder beveled proximate the weld has a double bevel forming a 30 degree included angle; in some embodiments, the anterior balloon beveled proximate the weld has a double bevel forming a 50 degree included angle; in some embodiments, the anterior bladder beveled proximate the weld has a double bevel forming an included angle of 65 degrees; in some embodiments, the anterior balloon beveled proximate the weld has a double bevel forming a 90 degree included angle; in some embodiments, the anterior bladder beveled proximate the weld has a double bevel forming an included angle of 120 degrees; the double inclined planes are respectively suitable for the puncture operation of the sample injection needle and the exhaust needle.

In some embodiments, the cylindrical balloon is further divided into a post-fill weld reserve, a weld assist clip reserve, a buffer space portion, and a sample loading portion.

In some embodiments, the overall height of the cryopreserved capsule is 50 to 200mm; in some embodiments, the overall height of the cryopreserved capsule is 60 to 160mm; in some embodiments, the overall height of the cryopreserved capsule is 70 to 110mm; in some embodiments, the overall height of the cryopreserved capsule is 50mm; in some embodiments, the overall height of the cryopreserved capsule is 60mm; in some embodiments, the overall height of the cryopreserved capsule is 70mm; in some embodiments, the overall height of the cryopreserved capsule is 73mm; in some embodiments, the overall height of the cryopreserved capsule is 87mm; in some embodiments, the overall height of the cryopreserved capsule is 104mm; in some embodiments, the overall height of the cryopreserved capsule is 110mm; in some embodiments, the overall height of the cryopreserved capsule is 160mm; in some embodiments, the overall height of the cryopreserved capsule is 200mm.

In some embodiments, the height of the weld is 1 to 20mm; in some embodiments, the height of the weld is 2 to 16mm; in some embodiments, the height of the weld is 2 to 10mm; in some embodiments, the height of the weld is 1mm; in some embodiments, the height of the weld is 2mm; in some embodiments, the height of the weld is 4mm; in some embodiments, the height of the weld is 6mm; in some embodiments, the height of the weld is 8mm; in some embodiments, the height of the weld is 10mm; in some embodiments, the height of the weld is 12mm; in some embodiments, the height of the weld is 16mm; in some embodiments, the height of the weld is 20mm.

In some embodiments, the height of the anterior capsule forming the bevel is 5-30 mm; in some embodiments, the height of the anterior capsule forming the bevel is 5-25 mm; in some embodiments, the height of the anterior capsule forming the bevel is 7-23 mm; in some embodiments, the height of the beveled anterior capsule is 5mm; in some embodiments, the height of the beveled anterior capsule is 7mm; in some embodiments, the height of the beveled anterior capsule is 16mm; in some embodiments, the height of the beveled anterior capsule is 23mm; in some embodiments, the height of the beveled anterior capsule is 25mm; in some embodiments, the height of the beveled anterior capsule is 30mm.

In some embodiments, the height of the cylindrical balloon is 30 to 150mm; in some embodiments, the height of the cylindrical balloon is 40 to 100mm; in some embodiments, the height of the cylindrical balloon is 50-70 mm; in some embodiments, the height of the cylindrical balloon is 30mm; in some embodiments, the height of the cylindrical balloon is 40mm; in some embodiments, the height of the cylindrical balloon is 50mm; in some embodiments, the height of the cylindrical balloon is 51mm; in some embodiments, the height of the cylindrical balloon is 54mm; in some embodiments, the height of the cylindrical balloon is 55mm; in some embodiments, the height of the cylindrical balloon is 60mm; in some embodiments, the height of the cylindrical balloon is 70mm; in some embodiments, the height of the cylindrical balloon is 100mm; in some embodiments, the height of the cylindrical balloon is 150mm.

In some embodiments, the cylindrical balloon has an outer diameter of 5 to 30mm; in some embodiments, the cylindrical balloon has an outer diameter of 10 to 24mm; in some embodiments, the cylindrical balloon has an outer diameter of 10 to 20mm; in some embodiments, the outer diameter of the cylindrical balloon is 5mm; in some embodiments, the outer diameter of the cylindrical balloon is 8mm; in some embodiments, the outer diameter of the cylindrical balloon is 10mm; in some embodiments, the outer diameter of the cylindrical balloon is 14mm; in some embodiments, the outer diameter of the cylindrical balloon is 18mm; in some embodiments, the outer diameter of the cylindrical balloon is 20mm; in some embodiments, the outer diameter of the cylindrical balloon is 24mm; in some embodiments, the outer diameter of the cylindrical balloon is 30mm.

In some embodiments, the beveled anterior capsule has a thickness of 0.5 to 3mm; in some embodiments, the beveled anterior capsule has a thickness of 0.5 to 1.5mm; in some embodiments, the beveled anterior balloon has a thickness of 0.75 to 1mm; in some embodiments, the beveled anterior capsule has a thickness of 0.5mm; in some embodiments, the beveled anterior capsule has a thickness of 0.75mm; in some embodiments, the beveled anterior capsule has a thickness of 1mm; in some embodiments, the beveled anterior capsule has a thickness of 1.25mm; in some embodiments, the beveled anterior capsule has a thickness of 1.5mm; in some embodiments, the beveled anterior capsule has a thickness of 2mm; in some embodiments, the beveled anterior capsule has a thickness of 2.5mm; in some embodiments, the beveled anterior capsule has a thickness of 3mm.

In some embodiments, the cylindrical balloon has a thickness of 0.5 to 3mm; in some embodiments, the cylindrical balloon has a thickness of 0.5 to 1.5mm; in some embodiments, the cylindrical balloon has a thickness of 0.75 to 1mm; in some embodiments, the thickness of the cylindrical balloon is 0.5mm; in some embodiments, the thickness of the cylindrical balloon is 0.75mm; in some embodiments, the cylindrical balloon has a thickness of 1mm; in some embodiments, the cylindrical balloon has a thickness of 1.25mm; in some embodiments, the cylindrical balloon has a thickness of 1.5mm; in some embodiments, the cylindrical balloon has a thickness of 2mm; in some embodiments, the cylindrical balloon has a thickness of 2.5mm; in some embodiments, the cylindrical balloon has a thickness of 3mm.

In some embodiments, the weld reserve after filling is provided as a capsule in the front of the cylindrical capsule, the length of which is 15-45% of the length of the cylindrical capsule; in some embodiments, the length of the weld reserve after filling is 15-45% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 25-40% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 15% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 20% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 25% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 27% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 30% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 37% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 39% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 40% of the length of the cylindrical bladder; in some embodiments, the length of the weld reserve after filling is 45% of the length of the cylindrical bladder.

In some embodiments, the welding auxiliary clamp reservation is provided as a balloon between the filled welding reservation and the buffer space, and the length of the balloon is 5 to 15% of the length of the cylindrical balloon; in some embodiments, the length of the welding auxiliary clamp reservation is 5-15% of the length of the cylindrical capsule body; in some embodiments, the length of the welding auxiliary clamp reservation part accounts for 7-12% of the length of the cylindrical capsule body; in some embodiments, the weld aid clip reserve has a length of 5% of the length of the cylindrical bladder; in some embodiments, the weld aid clip reserve has a length of 7% of the length of the cylindrical bladder; in some embodiments, the weld aid clip reserve has a length of 9% of the length of the cylindrical bladder; in some embodiments, the weld aid clip reserve has a length of 10% of the length of the cylindrical bladder; in some embodiments, the weld aid clip reserve has a length of 12% of the length of the cylindrical bladder; in some embodiments, the weld aid clip reserve has a length of 15% of the length of the cylindrical balloon.

In some embodiments, the buffer space part is provided as a capsule body between the welding auxiliary clamp reservation part and the loading part, and the length of the capsule body accounts for 1-25% of the length of the cylindrical capsule body; in some embodiments, the length of the cushioning space portion is 1 to 25% of the length of the cylindrical bladder; in some embodiments, the length of the cushioning space portion is 5 to 22% of the length of the cylindrical bladder; in some embodiments, the length of the cushioning space portion is 1% of the length of the cylindrical bladder; in some embodiments, the length of the cushioning space portion is 5% of the length of the cylindrical balloon; in some embodiments, the length of the cushioning space portion is 6% of the length of the cylindrical bladder; in some embodiments, the length of the cushioning space portion is 16% of the length of the cylindrical balloon; in some embodiments, the length of the cushioning space portion is 20% of the length of the cylindrical bladder; in some embodiments, the length of the cushioning space portion is 22% of the length of the cylindrical bladder; in some embodiments, the length of the cushioning space portion is 25% of the length of the cylindrical bladder.

In some embodiments, the sample loading portion is provided as a capsule at the rear of the cylindrical capsule, and the length of the capsule is 25-70% of the length of the cylindrical capsule; in some embodiments, the sample loading portion has a length of 25 to 70% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 30-60% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 25% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 30% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 32% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 35% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length that is 40% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length that is 50% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 55% of the length of the cylindrical capsule; in some embodiments, the sample loading portion has a length of 58% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 60% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 65% of the length of the cylindrical balloon; in some embodiments, the sample loading portion has a length of 70% of the length of the cylindrical balloon.

In some embodiments, the welding portion at two ends or one end of the frozen capsule, the front capsule body with two ends or one end adjacent to the welding portion to form the inclined surface, and the middle or one end cylindrical capsule body are integrally formed, wherein the materials used for each part are selected from Ethylene-vinyl acetate Copolymer (Ethylene vinyl acetate Copolymer, EVA) or perfluoroethylene propylene Copolymer (FEP).

The utility model provides a pair of a cryopreserved capsule for cell product is preserved can also further include unable adjustment base.

In some embodiments, the fixture base is shaped and dimensioned as a cylindrical or clamp-like structure into which a weld portion adapted to freeze an end of the capsule, a front bladder body beveled proximate the weld portion, a buffer space portion of the cylindrical bladder body, and the sample loading portion are inserted for fixation.

In some embodiments, one end of the fixing base is of a sealing structure, and the other end of the fixing base is of an opening structure.

In some embodiments, the closed end of the fixing base is provided with a supporting structure which is in matched contact with the front bag body forming the inclined plane and an open slot for allowing the welding part to pass through, or the closed end is provided with a flat bottom structure.

In some embodiments, the height of the fixing base is set to be identical to the height at which the upper end of the buffering space part is located.

The technical effects are as follows: the cryopreservation capsule of the utility model can be made of a single material, and can realize the automatic production and filling of cell products without the need of component design with a complex structure; particularly, through the selection and optimization of the size, the thickness and the material of the packaging container, the packaging container can be easily matched with an automatic filling production line on the basis of keeping proper rigidity (standability) and temperature conduction efficiency (low-temperature freezing storage efficiency), the high-speed filling of a sample is easily realized in structural design, and the high-efficiency automatic production is facilitated; the integrated molding is adopted, encapsulation and exhaust can be carried out under the completely sealed condition, and the risks of leakage and pollution in the production, manufacture, storage and transportation processes of biological products such as cells and the like can be avoided to the maximum extent; the integrated totally-enclosed structure is realized, and the low-temperature cryopreservation at minus 196 ℃ in a liquid nitrogen environment can be endured for a long time.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive labor.

Fig. 1 is a front view of the cryopreservation capsule of the present invention for preserving cell products.

Fig. 2 is a side view of the cryopreservation capsule for cell product preservation of the present invention.

Fig. 3 is a top view of the cryopreservation capsule for storing cell products of the present invention.

Fig. 4 is a working view of the present invention when filling a cryopreservation capsule for preserving cell products.

Fig. 5 is a front view of the cryopreserved capsule for cell product preservation after filling.

Fig. 6 is a filled rear side view of the cryopreservation capsule for preserving cell products of the present invention.

Fig. 7 is a top view of the filled cryopreserved capsule for storing cell products of the present invention.

Fig. 8 is a front view of a cryopreserved capsule (variant) for cell product preservation according to the present invention.

Fig. 9 is a side view of a cryopreserved capsule (variant) for cell product preservation according to the present invention.

Fig. 10 is a top view of a cryopreserved capsule (variant) for cell product preservation according to the present invention.

Fig. 11 is a working view of the filling of the cryopreservation capsule (variant) for cell product preservation according to the present invention.

Fig. 12 is a filled front view of a cryopreserved capsule (variant) for cell product preservation according to the present invention.

Fig. 13 is a side view of the filled cryopreserved capsule (variant) for cell product preservation according to the present invention.

Fig. 14 is a top view of the filled cryopreserved capsule (variant) for cell product preservation according to the present invention.

Reference numerals: 1 welding part, 2 front capsule body with inclined plane, 3 cylindrical capsule body, 4 welding reserved part after filling, 5 welding auxiliary clamp reserved part, 6 buffer space part, 7 sample loading part, 8 fixing base, 9 supporting structure, 10 open slot, 11 sample injection needle, 12 exhaust needle, 13 auxiliary clamp, 14 welding clamp, 41 welding part after filling, and 42 front capsule body with inclined plane after filling.

Detailed Description

The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following embodiments are only preferred embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution of the present invention within the technical scope disclosed in the present invention.

As shown in fig. 1 to 7, example 1 provides a cryopreservation capsule for cell product preservation, comprising welding parts 1 at both ends of the capsule, a front capsule body 2 with both ends formed with slopes next to the welding parts 1, and a middle cylindrical capsule body 3; wherein, the welding parts 1 at the two ends of the capsule are of a totally-enclosed structure which is realized by welding through a high-frequency heat seal process; wherein, the front capsule body 2 with two ends adjacent to the welding part 1 to form inclined planes has double inclined planes forming an included angle of 90 degrees, and the double inclined planes are respectively suitable for the puncture operation of the sample injection needle 11 and the exhaust needle 12; wherein, the middle cylindrical capsule body 3 is further divided into a welding reserved part 4 after filling, a welding auxiliary clamp reserved part 5, a buffer space part 6 and a sample loading part 7; wherein the overall height of the frozen capsule is 73mm; wherein, the height of the welding parts 1 at the two ends of the capsule is 2mm; wherein, the height of the front capsule body 2 with two ends forming a slope is 7mm; wherein, the height of the middle cylindrical capsule body 3 is 55mm; wherein the outer diameter of the middle cylindrical capsule body 3 is 10mm; wherein, the thickness of the front capsule body 2 with two inclined surfaces at two ends is 0.75mm; wherein the thickness of the middle cylindrical capsule body 3 is 0.75mm; wherein, the welding reserved part 4 is arranged as a capsule body at the front part of the middle cylindrical capsule body 3 after filling, and the length of the capsule body is 27 percent of the length of the middle cylindrical capsule body; wherein, the welding auxiliary clamp reserved part 5 is arranged as a capsule body between the filled welding reserved part 4 and the buffer space part 6, and the length of the capsule body is 9 percent of the length of the middle cylindrical capsule body; wherein, the buffer space part 6 is arranged as a capsule body between the welding auxiliary clamp reservation part 5 and the loading part 7, and the length of the capsule body is 6 percent of the length of the middle cylindrical capsule body; wherein, the sample loading part 7 is arranged as a capsule body at the rear part of the middle cylindrical capsule body 3, and the length of the sample loading part is 58 percent of the length of the middle cylindrical capsule body; the welding parts 1 at two ends of the frozen capsule, the front capsule body 2 with two ends adjacent to the welding parts 1 to form inclined planes and the middle cylindrical capsule body 3 are integrally formed, and the materials used by all the parts are Ethylene-Vinyl Acetate Copolymer (EVA for short).

As shown in fig. 1 to 7, example 2 provides a cryopreservation capsule for cell product preservation, comprising welding parts 1 at both ends of the capsule, a front capsule body 2 with both ends formed with slopes next to the welding parts 1, and a middle cylindrical capsule body 3; wherein, the welding parts 1 at the two ends of the capsule are of a totally-enclosed structure which is realized by welding through a high-frequency heat seal process; wherein, the front capsule body 2 with two ends adjacent to the welding part 1 to form inclined planes has double inclined planes forming an included angle of 65 degrees, and the double inclined planes are respectively suitable for the puncture operation of the sample injection needle 11 and the exhaust needle 12; wherein, the middle cylindrical capsule body 3 is further divided into a welding reserved part 4 after filling, a welding auxiliary clamp reserved part 5, a buffer space part 6 and a sample loading part 7; wherein the overall height of the frozen capsule is 87mm; wherein, the height of the welding parts 1 at the two ends of the capsule is 2mm; wherein, the height of the front capsule body 2 with two ends forming a slope is 16mm; wherein the height of the middle cylindrical capsule body 3 is 51mm; wherein the outer diameter of the middle cylindrical capsule body 3 is 18mm; wherein, the thickness of the front capsule body 2 with two ends forming inclined planes is 1mm; wherein, the thickness of the middle cylindrical capsule body 3 is 1mm; wherein, the welding reserved part 4 is arranged as a capsule body at the front part of the middle cylindrical capsule body 3 after filling, and the length of the capsule body is 39 percent of the length of the middle cylindrical capsule body; wherein, the welding auxiliary clamp reserved part 5 is arranged as a capsule body between the filled welding reserved part 4 and the buffer space part 6, and the length of the capsule body is 10 percent of the length of the middle cylindrical capsule body; wherein, the buffer space part 6 is arranged as a capsule body between the welding auxiliary clamp reservation part 5 and the loading part 7, and the length of the capsule body is 16 percent of the length of the middle cylindrical capsule body; wherein, the sample loading part 7 is arranged as a capsule body at the rear part of the middle cylindrical capsule body 3, and the length of the sample loading part is 35 percent of the length of the middle cylindrical capsule body; wherein, the welding parts 1 at two ends of the frozen capsule, the front capsule body 2 with two ends adjacent to the welding parts 1 to form a slope and the middle cylindrical capsule body 3 are integrally formed, and the material used for each part is Ethylene-Vinyl Acetate Copolymer (English full name: ethylene Vinyl Acetate Copolymer, english short for EVA).

As shown in fig. 1 to 7, example 3 provides a cryopreservation capsule for cell product preservation, comprising welding parts 1 at both ends of the capsule, a front capsule body 2 with both ends formed with slopes next to the welding parts 1, and a middle cylindrical capsule body 3; wherein, the welding parts 1 at the two ends of the capsule are of a totally-enclosed structure which is realized by welding through a high-frequency heat seal process; wherein, the front capsule body 2 with two ends adjacent to the welding part 1 to form inclined planes has double inclined planes with an included angle of 50 degrees, and the double inclined planes are respectively suitable for the puncture operation of the sample injection needle 11 and the exhaust needle 12; wherein, the middle cylindrical capsule body 3 is further divided into a welding reserved part 4 after filling, a welding auxiliary clamp reserved part 5, a buffer space part 6 and a sample loading part 7; wherein the overall height of the frozen capsule is 104mm; wherein, the height of the welding parts 1 at the two ends of the capsule is 2mm; wherein, the height of the front capsule body 2 with two ends forming a slope is 23mm; wherein, the height of the middle cylindrical capsule body 3 is 54mm; wherein, the outer diameter of the middle cylindrical capsule body 3 is 24mm; wherein, the thickness of the front capsule body 2 with two ends forming inclined planes is 1mm; wherein, the thickness of the middle cylindrical capsule body 3 is 1mm; wherein, the welding preserved part 4 is arranged as the capsule body at the front part of the middle cylindrical capsule body 3 after filling, and the length of the capsule body is 37 percent of the length of the middle cylindrical capsule body; wherein, the welding auxiliary clamp reserved part 5 is arranged as a capsule body between the filled welding reserved part 4 and the buffer space part 6, and the length of the capsule body is 9 percent of the length of the middle cylindrical capsule body; wherein, the buffer space part 6 is arranged as a capsule body between the welding auxiliary clamp reservation part 5 and the loading part 7, and the length of the capsule body is 22 percent of the length of the middle cylindrical capsule body; wherein, the sample loading part 7 is arranged as a capsule body at the rear part of the middle cylindrical capsule body 3, and the length of the sample loading part is 32 percent of the length of the middle cylindrical capsule body; wherein, the welding parts 1 at two ends of the frozen capsule, the front capsule body 2 with two ends adjacent to the welding parts 1 to form a slope and the middle cylindrical capsule body 3 are integrally formed, and the material used for each part is Ethylene-Vinyl Acetate Copolymer (English full name: ethylene Vinyl Acetate Copolymer, english short for EVA).

As shown in fig. 8 to 14, example 4 provides a cryopreservation capsule for cell product preservation, comprising a welding part 1 at one end of the capsule, a front capsule body 2 with one end being formed with a slope next to the welding part 1, and a cylindrical capsule body 3 with one end; wherein, the welding part 1 at one end of the capsule is a totally-enclosed structure which is realized by welding through a laser welding process; wherein, the front capsule body 2 with one end adjacent to the welding part 1 to form a slope has a double slope forming an included angle of 50 degrees, and the double slopes are respectively suitable for the puncture operation of the sample injection needle 11 and the exhaust needle 12; wherein, the cylindrical capsule body 3 at one end is further divided into a welding preserved part 4 after filling, a welding auxiliary clamp preserved part 5, a buffer space part 6 and a sample loading part 7; wherein the overall height of the frozen capsule is 79mm; wherein, the height of the welding part 1 at one end of the capsule is 2mm; wherein, the height of the front capsule body 2 with one end forming a slope is 23mm; wherein, the height of the cylindrical capsule body 3 at one end is 54mm; wherein, the outer diameter of the cylindrical capsule body 3 at one end is 24mm; wherein, the thickness of the front capsule body 2 with one end forming a slope is 1mm; wherein, the thickness of the cylindrical capsule body 3 at one end is 1mm; wherein, the welding preserved part 4 is arranged as a capsule body at the front part of the cylindrical capsule body 3 at one end, and the length of the capsule body is 37 percent of that of the cylindrical capsule body at one end; the welding auxiliary clamp reserved part 5 is a capsule body arranged between the filled welding reserved part 4 and the buffer space part 6, and the length of the capsule body is 9% of that of a cylindrical capsule body at one end; wherein, the buffer space part 6 is arranged as a capsule body between the welding auxiliary clamp reservation part 5 and the loading part 7, and the length of the capsule body is 22 percent of the length of a cylindrical capsule body at one end; wherein, the sample loading part 7 is arranged as a capsule body at the rear part of the cylindrical capsule body 3 at one end, and the length of the sample loading part is 32 percent of the length of the cylindrical capsule body at one end; the welding part 1 at one end of the frozen capsule, the front capsule body 2 with one end adjacent to the welding part 1 to form a slope and the cylindrical capsule body 3 at one end are integrally molded, and the materials used for each part are perfluoroethylene propylene copolymer (FEP).

The cryopreservation capsule for cell product preservation provided by the above embodiments 1 to 4 can be further provided with a fixed base 8; wherein, the fixed base 8 is a cylindrical structure which is suitable for freezing and storing the welding part 1 at one end of the capsule, the front capsule body 2 which is adjacent to the welding part 1 and forms an inclined plane, the buffer space part 6 of the cylindrical capsule body 3 and the sample loading part 7 to be inserted and fixed; wherein, one end of the fixed base 8 is a sealing structure, and the other end is an opening structure; wherein, the sealing end of the fixed base 8 is provided with a supporting structure 9 which is matched and contacted with the front bag body 2 forming an inclined plane and an open slot 10 which allows the welding part 1 to pass through; or the sealing end is a flat bottom structure; wherein the height of the fixing base 8 is set to be identical to the height at which the upper end of the buffering space part 6 is located.

The utility model discloses a freeze concrete using-way of depositing capsule for cell product is: the frozen capsule is loaded and fixed in a fixed base 8 in an upright state, wherein a front capsule body 2 with the lower end adjacent to a welding part 1 to form an inclined plane is supported by a supporting structure 9 in the fixed base 8, and the welding part 1 at the lower end is inserted into an open slot 10 in the fixed base 8; the frozen capsule after being loaded and fixed is placed in a filling line, two inclined planes of a front capsule body 2 with the upper end adjacent to a welding part 1 to form an inclined plane are simultaneously punctured by a sample injection needle 11 and an exhaust needle 12 respectively, the puncture depth of the sample injection needle 11 is that a needle head is positioned in a welding auxiliary clamp reserved part 5, the puncture depth of the exhaust needle 12 is that the needle head is positioned in a welding reserved part 4 after filling, and then sample filling is synchronously carried out through the sample injection needle 11 and internal gas exhaust operation is carried out through the exhaust needle 12; after the filling and exhausting operations are finished, the auxiliary clamp 13 clamps and welds the auxiliary clamp reserved part 5 at a relatively low speed (the part can be prevented from splashing due to overlarge capsule body deformation by the low-speed clamping of the auxiliary clamp 13, part of air is removed in the clamping process, a relative negative pressure state is given to the sealed capsule body, the auxiliary clamp 13 can also be linked with a temperature control device at 2-8 ℃ to prevent the influence of high temperature in the welding process on the bioactivity of the filled sample), and then the welding clamp 14 clamps and welds the filled welding reserved part 4 at a relatively high speed for high-frequency heat seal welding (or laser welding), so that a filled welding part 41 and a filled capsule body front part 42 which is adjacent to the welding part 41 and forms an inclined plane are formed; after welding, the welding part 1 at the original upper end and the front capsule body 2 with the original upper end adjacent to the welding part 1 to form the inclined plane are cut off along the upper end of the filled welding mark, and the filled welding part 41 and the front capsule body 42 with the inclined plane adjacent to the welding part 41 after filling are reserved.

Claims (46)

1. A freezing capsule for preserving cell products is characterized by comprising welding parts (1) positioned at two ends or one end of the capsule, a front capsule body (2) with two ends or one end being adjacent to the welding parts (1) to form an inclined surface, and a cylindrical capsule body (3) in the middle or one end; the welding part (1) is a totally-enclosed structure which is welded by a high-frequency heat seal process or a laser welding process; wherein the front bag body (2) which is adjacent to the welding part (1) and forms an inclined plane has a double inclined plane which forms an included angle of 30-120 degrees; wherein the cylindrical capsule body (3) is divided into a welding reserved part (4) after filling, a welding auxiliary clamp reserved part (5), a buffer space part (6) and a sample loading part (7).

2. The cryopreservation capsule of claim 1, wherein the front capsule body (2) forming a bevel next to the weld (1) has a double bevel forming an included angle of 50-90 degrees.

3. The cryopreservation capsule of claim 1, wherein the front capsule body (2) beveled proximate to the weld (1) has a double bevel forming an included angle of 30, 50, 65, 90 or 120 degrees.

4. The cryopreserved capsule of claim 1, wherein the overall height of the cryopreserved capsule is 50-200 mm.

5. The cryopreservation capsule of claim 4, wherein the overall height of the cryopreservation capsule is from 60 to 160mm.

6. The cryopreservation capsule of claim 5, wherein the overall height of the cryopreservation capsule is 70 to 110mm.

7. The cryopreservation capsule of claim 4, wherein the overall height of the cryopreservation capsule is 50mm, 60mm, 70mm, 73mm, 87mm, 104mm, 110mm, 160mm or 200mm.

8. The cryopreservation capsule of claim 1, wherein the height of the welding portion (1) is 1 to 20mm.

9. The cryopreservation capsule of claim 8, wherein the height of the welding portion (1) is 2-16 mm.

10. The cryopreservation capsule of claim 9, wherein the height of the welding portion (1) is 2-10 mm.

11. The cryopreservation capsule of claim 8, wherein the height of the weld (1) is 1mm, 2mm, 4mm, 6mm, 8mm, 10mm, 12mm, 16mm or 20mm.

12. The cryopreservation capsule of claim 1, wherein the height of the beveled front capsule body (2) is 5-30 mm.

13. The cryopreservation capsule of claim 12, wherein the height of the beveled front capsule body (2) is 5-25 mm.

14. The cryopreserved capsule according to claim 13, wherein the height of the beveled front bladder (2) is 7-23 mm.

15. The cryopreservation capsule of claim 12, wherein the beveled anterior capsule (2) has a height of 5mm, 7mm, 16mm, 23mm, 25mm or 30mm.

16. The cryopreservation capsule according to claim 1, wherein the height of the cylindrical capsule body (3) is 30-150 mm.

17. The cryopreservation capsule of claim 16, wherein the height of the cylindrical body (3) is 40-100 mm.

18. The cryopreservation capsule of claim 17, wherein the height of the cylindrical body (3) is 50-70 mm.

19. The cryopreserved capsule according to claim 16, wherein the height of the cylindrical body (3) is 30mm, 40mm, 50mm, 51mm, 54mm, 55mm, 60mm, 70mm, 100mm or 150mm.

20. The cryopreservation capsule according to claim 1, wherein the outer diameter of the cylindrical capsule body (3) is 5-30 mm.

21. The cryopreservation capsule of claim 20, wherein the outer diameter of the cylindrical capsule body (3) is 10-24 mm.

22. The cryopreservation capsule of claim 21, wherein the outer diameter of the cylindrical capsule body (3) is 10-20 mm.

23. The cryopreservation capsule of claim 20, wherein the outer diameter of the cylindrical capsule body (3) is 5mm, 8mm, 10mm, 14mm, 18mm, 20mm, 24mm or 30mm.

24. The cryopreservation capsule of claim 1, wherein the thickness of the beveled front capsule body (2) is 0.5-3 mm.

25. The cryopreservation capsule of claim 24, wherein the thickness of the beveled front capsule body (2) is 0.5-1.5 mm.

26. The cryopreservation capsule of claim 25, wherein the thickness of the beveled front capsule body (2) is 0.75-1 mm.

27. The cryopreservation capsule of claim 24, wherein the thickness of the beveled anterior capsule body (2) is 0.5mm, 0.75mm, 1mm, 1.25mm, 1.5mm, 2mm, 2.5mm or 3mm.

28. The cryopreservation capsule according to claim 1, wherein the thickness of the cylindrical body (3) is 0.5-3 mm.

29. The cryopreservation capsule of claim 28, wherein the thickness of the cylindrical body (3) is 0.5-1.5 mm.

30. The cryopreservation capsule of claim 29, wherein the thickness of the cylindrical body (3) is 0.75-1 mm.

31. The cryopreservation capsule of claim 28, wherein the thickness of the cylindrical capsule body (3) is 0.5mm, 0.75mm, 1mm, 1.25mm, 1.5mm, 2mm, 2.5mm or 3mm.

32. The cryopreserved capsule according to claim 1, wherein the length of the post-filling weld reserve (4) is 15-45% of the length of the cylindrical shell (3).

33. The cryopreserved capsule according to claim 32, wherein the length of the post-fill weld reserve (4) is 25-40% of the length of the cylindrical body (3).

34. The cryopreservation capsule of claim 32, wherein the length of the post-filling weld reserve (4) is 15%, 20%, 25%, 27%, 30%, 37%, 39%, 40% or 45% of the length of the cylindrical capsule body (3).

35. The cryopreservation capsule according to claim 1, wherein the length of the welding-aid clip reserve (5) is 5-15% of the length of the cylindrical capsule body (3).

36. The cryopreservation capsule according to claim 35, wherein the length of the welding-aid clip reserve (5) is 7-12% of the length of the cylindrical capsule body (3).

37. The cryopreservation capsule of claim 35, wherein the weld assist clip reserve (5) has a length of 5%, 7%, 9%, 10%, 12% or 15% of the length of the cylindrical capsule body (3).

38. The cryopreservation capsule according to claim 1, wherein the length of the buffer space portion (6) is 1 to 25% of the length of the cylindrical capsule body (3).

39. The cryopreservation capsule of claim 38, wherein the length of the buffer space portion (6) is 5-22% of the length of the cylindrical capsule body (3).

40. The cryopreserved capsule according to claim 38, wherein the length of the buffer space portion (6) is 1%, 5%, 6%, 16%, 20%, 22% or 25% of the length of the cylindrical capsule body (3).

41. The cryopreservation capsule of claim 1, wherein the length of the sample loading portion (7) is 25-70% of the length of the cylindrical capsule body (3).

42. The cryopreservation capsule of claim 41, wherein the length of the sample loading portion (7) is 30-60% of the length of the cylindrical capsule body (3).

43. The cryopreservation capsule of claim 41, wherein the length of the sample loading portion (7) is 25%, 30%, 32%, 35%, 40%, 50%, 55%, 58%, 60%, 65% or 70% of the length of the cylindrical capsule body (3).

44. The cryopreservation capsule according to claim 1, wherein the welding part (1) at two ends or one end of the cryopreservation capsule, the front capsule body (2) with two ends or one end adjacent to the welding part (1) and forming a slope, and the cylindrical capsule body (3) at the middle or one end are integrally formed, and the materials of all parts are selected from ethylene-vinyl acetate copolymer or perfluoroethylene propylene copolymer.

45. The cryopreservation capsule of claim 1, further comprising a fixed base (8).

46. The cryopreservation capsule of claim 45, wherein the fixing base (8) is shaped and dimensioned as a cylinder or clamp-like structure into which a welding part (1) adapted to one end of the cryopreservation capsule, a front capsule body (2) beveled next to the welding part (1), a buffer space part (6) and a sample loading part (7) of a cylindrical capsule body (3) are inserted and fixed; one end of the fixed base (8) is of a sealing structure, the other end of the fixed base is of an opening structure, and the sealing end is provided with a supporting structure (9) which is matched and contacted with the front bag body (2) forming the inclined plane and an open slot (10) for the welding part (1) to pass through, or is of a flat bottom structure; the height of the fixing base (8) is set to be identical to the height of the upper end of the buffer space part (6).

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