CN218617859U

CN218617859U - Box for whole membrane incubation

Info

Publication number: CN218617859U
Application number: CN202220669279.2U
Authority: CN
Inventors: 刘孟楠; 杨思进; 罗钢; 刘颜; 刘佳利; 韩梅; 杨廷富
Original assignee: Affiliated Hospital of Traditional Chinese Medicine TCM of Southwest Medical University
Current assignee: Affiliated Hospital of Traditional Chinese Medicine TCM of Southwest Medical University
Priority date: 2022-03-26
Filing date: 2022-03-26
Publication date: 2023-03-14
Anticipated expiration: 2032-03-26

Abstract

The utility model belongs to the field of medical appliance articles, and discloses a box for film finishing incubation, which comprises a box body, wherein one side of the box body is hinged with a box cover, a multi-antibody film finishing is placed in the box body, two sides of the bottom of the box body are provided with arc-shaped slideways, the arc-shaped slideways are connected with a slide block in a sliding manner, a positioning hole is arranged on the contact surface of the slide block and the side wall of the box body, the side wall of the box body is provided with a plurality of limiting holes, the side wall of the box body is connected with a positioning pin, the positioning pin passes through the limiting holes and the positioning hole, an inserting plate groove is arranged in the slide block, a partition plate is placed in the inserting plate groove, and a pressing mechanism is arranged above the partition plate; the utility model provides a prior art can not adjust the problem of baffle position wantonly, the utility model discloses an adjust the position of baffle for the incubation space in the box body can be adjusted, and need not tailor the membrane, can directly incubate different antibodies in different positions.

Description

Box for whole membrane incubation

Technical Field

The utility model relates to a molecular biology experiment technical field especially relates to a box that is used for whole membrane to incubate.

Background

Western blot (immunoblot assay), western blot, is a commonly used experimental method in molecular biology, biochemistry and immunogenetics, and its basic principle is to stain a gel electrophoresis-treated cell or biological tissue sample with a specific antibody. Information on the expression of a specific protein in the analyzed cell or tissue is obtained by analyzing the location and depth of staining. Since western blotting experiments have high resolution and high specificity and sensitivity of solid-phase immunoassay, it has become a basic technology for protein analysis experiments in laboratories related to the medical and biological fields. In western blot experiments, antibody incubation is an important step.

Present antibody incubation box is mostly fixed cellular-type incubation box, often all need cut into different rectangle strips with PVDF membrane according to the molecular weight size earlier, put into the separating tank of range and add different antibodies and incubate, the strip of final exposure is not on same membrane, and be difficult to judge the size of all purpose protein strips according to protein mark Marker, take trouble and difficultly, great operation degree of difficulty and operating time have not only been increased, and be difficult to satisfy stricter scientific research standard requirement day by day, some automatic incubation devices that present can not solve the purpose of incubating a plurality of antibodies simultaneously on same membrane, and present incubation box often the volume can not be adjusted, can not reach the purpose that the experimenter practices thrift the antibody.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a box for whole mould is hatched to solve prior art and be used not to adjust the baffle position problem wantonly.

In order to achieve the above object, the present invention provides the following technical solutions: the utility model provides a box for whole membrane is hatched, comprises a box bod, one side of box body articulates there is the lid, the box body is a cavity, placed the whole membrane of many antibodies in the box body, box body bottom both sides are equipped with the arc slide, arc slide sliding connection has the slider, be equipped with the locating hole on the contact surface of slider and box body lateral wall, the box body lateral wall is equipped with the spacing hole of a plurality of, be connected with the locating pin on the box body lateral wall, the locating pin passes spacing hole and locating hole, be equipped with the picture peg groove in the slider, the baffle has been placed to the picture peg inslot, the baffle top is equipped with the hold-down mechanism who makes baffle and box body closely laminate.

The principle of the technical scheme is as follows: the both sides of box body bottom are equipped with the arc slide, and the slider can slide on the arc slide wantonly, is equipped with the locating hole on the slider, and the box body lateral wall is equipped with spacing hole, thereby the locating pin passes spacing hole and locating hole and makes the slider spacing, thereby the picture peg groove that the baffle inserted on the slider separates into a plurality of spaces of hatching with the box body, thereby the position of baffle has been adjusted to the regulating block position promptly.

The beneficial effects of the technical scheme are that: the incubation space in the box body can be adjusted by adjusting the position of the partition plate; different antibodies can be directly incubated at different positions without cutting the membrane; the tightness of each incubation space is ensured by arranging the pressing mechanism.

Preferably, the pressing mechanism comprises a first pressing rod and a second pressing rod, the first pressing rod is arranged above the second pressing rod, a spring is connected between the first pressing rod and the second pressing rod, a threaded hole is formed in the sliding block, the first pressing rod is rotatably connected with a bolt, the bolt is in threaded connection with the threaded hole, and a groove matched with the top of the partition board is formed in the bottom of the second pressing rod.

Through the setting, put into the baffle top with hold-down mechanism, behind the baffle inserted the picture peg groove on the slider, the recess cooperation baffle of second compression rod's top, thereby the bolt on the first compression rod and the screw hole threaded connection spring compression on the slider compress tightly the pressure for the second compression rod, and the second compression rod is exerted pressure again and is given the baffle for the whole membrane contact of baffle and many antibodies is inseparabler.

Preferably, the contact junction of the partition plate and the inserting plate groove is provided with a sealing strip for blocking liquid, and the contact junction of the partition plate and the whole multi-antibody membrane is provided with a sealing strip.

Through the setting, obstruct the liquid circulation to make in different incubation space incubate different antibodies.

Preferably, a clamping groove matched with the first pressing rod is formed in the box cover.

Through the arrangement, the box cover can be tightly covered on the box body, and the airtightness of each incubation space separated by the partition plate is ensured.

Preferably, the box body is made of transparent materials, and scales used for measuring and displaying the distance between the partition plates are arranged on the side wall of the box body.

Through the arrangement, the distance between the partition plates can be observed and measured, and meanwhile, an operator can conveniently adjust the distance of the partition plates according to needs.

Preferably, the height of the arc-shaped slide way is greater than the height of liquid in the box body.

Through the setting, thereby prevent that liquid from flowing extravagant liquid on the arc slide, prevent in addition that the arc slide from influencing the experimentation.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the box body of the present invention;

FIG. 3 is an enlarged view of portion A of FIG. 2;

fig. 4 is a schematic structural view of the pressing mechanism of the present invention;

the names of the corresponding labels in the drawings are:

the box body 1, lid 2, arc slide 3, slider 4, locating hole 5, spacing hole 6, locating pin 7, picture peg groove 8, baffle 9, first pressure bar 10, second pressure bar 11, spring 12, screw hole 13, bolt 14, recess 15, sealing strip 16, scale 17.

Detailed Description

The invention is described in further detail below with reference to the following figures and embodiments: as shown in fig. 1-4, a box for whole membrane incubation comprises a box body 1, one side of the box body 1 is hinged with a box cover 2, the box cover 2 is made of opaque material, and the top surface of the box cover 2 can be used for oil pen writing; the outer top of the box cover 2 is provided with a limiting groove, and the outer bottom of the box body 1 is provided with a bulge matched with the limiting groove at the outer top of the box cover 2, so that a plurality of boxes are overlapped and are not easy to slide off when the shaking table shakes; the box body 1 is a cavity, the whole multi-antibody film is placed in the box body 1, the front side and the rear side of the bottom of the box body 1 are provided with arc-shaped slideways 3, and the number of the arc-shaped slideways 3 is two; in the embodiment, the arc-shaped slide ways 3 on the left side and the right side are respectively connected with three symmetrical slide blocks 4 in a sliding manner, six slide blocks 4 are three groups of slide blocks 4, and the slide blocks 4 can slide on the slide ways at will; each sliding block 4 is internally provided with an inserting plate groove 8, a partition plate 9 is placed in the inserting plate groove 8, a sealing strip 16 for blocking liquid is arranged at the contact joint of the partition plate 9 and the inserting plate groove 8, so that the liquid is prevented from entering the inserting plate groove 8 to influence the experiment, and meanwhile, the liquid is prevented from entering other incubation spaces through the inserting plate groove 8 to cause interference; each group of sliding blocks 4 corresponds to one partition board 9, namely three partition boards 9 are arranged, namely the box body 1 is divided into four incubation spaces; a positioning hole 5 is formed in the contact surface of each sliding block 4 and the side wall of the box body 1, 19 limiting holes 6 are formed in the left side wall and the right side wall of the box body 1 respectively, the diameters of the positioning holes 5 and the limiting holes 6 are the same or the diameter of each limiting hole 6 is slightly larger than that of each positioning hole 5, a positioning pin 7 is connected to the side wall of the box body 1, and the positioning pin 7 penetrates through the limiting holes 6 and the positioning holes 5 to enable the sliding blocks 4 not to slide any more; be equipped with on the lateral wall of box body 1 and be used for measuring the scale 17 that shows the distance between the baffle 9, thereby adjustable slider 4 position is adjusted the baffle 9 position as required for it is adjustable to hatch the space.

A pressing mechanism which enables the partition board 9 to be tightly attached to the box body 1 is arranged above the partition board 9, the pressing mechanism comprises a first pressing rod 10 and a second pressing rod 11, the first pressing rod 10 is arranged above a second pressing plate, the first pressing rod 10 is longer than the second pressing rod 11, a spring 12 is connected between the first pressing plate and the second pressing plate, threaded holes 13 are formed in the six sliders 4, bolts 14 are rotatably connected to the left end and the right end of the first pressing rod 10, the bolts 14 are in threaded connection with the threaded holes 13 in the sliders 4, and a groove 15 matched with the top of the partition board 9 is formed in the bottom of the second pressing rod 11; after the partition board 9 is inserted into the inserting board groove 8 on the sliding block 4, the groove 15 of the second pressing rod 11 is matched with the top of the partition board 9, the bolt 14 on the first pressing rod 10 is in threaded connection with the threaded hole 13 on the sliding block 4, so that the spring 12 is compressed to apply pressure to the second pressing rod 11, the second pressing rod 11 applies pressure to the partition board 9 again, the partition board 9 is in closer contact with the whole multi-antibody membrane, at the moment, the pressing mechanism has a certain height, the top in the box cover 2 is provided with a clamping groove matched with the first pressing rod 10, so that the first pressing rod 10 can be clamped into the clamping groove, the box cover 2 can be covered on the box body 1 for sealing, and the sealing performance of an incubation space partitioned by the partition board 9 is ensured; in addition, a sealing strip 16 is arranged at the contact joint of the partition board 9 and the whole multi-antibody membrane, so that better sealing performance is realized; the width of the sealing strip 16 contacting the membrane at the bottom of the partition 9 is not more than 1.5 mm, so that the area of the membrane occupied by the sealing strip 16 is reduced; the height of the arc-shaped slide way 3 is larger than the height of the liquid in the box body 1, so that the liquid is prevented from flowing onto the arc-shaped slide way 3.

The specific implementation process is as follows: the box cover 2 is opened, the whole multi-antibody film is placed into the box body 1, the positions of the sliding blocks 4 on the arc-shaped slide ways 3 are adjusted, the positioning pins 7 are inserted into the positioning holes 5 and the limiting holes 6, each group of sliding blocks 4 are symmetrically limited, the partition plates 9 are inserted into the inserting plate grooves 8 on the sliding blocks 4, the pressing mechanisms are reused, the tops of the partition plates 9 enter the grooves 15 on the second pressing rods 11, the bolts 14 on the first pressing rods 10 are screwed into the threads on the sliding blocks 4, finally, liquid is poured into the grooves for incubation, the box cover 2 is well covered for sealing, and the pressing mechanisms and the partition plates 9 can be removed after the completion.

The above description is only an example of the present invention, and the common general knowledge of the technical solutions and characteristics known in the solutions is not described herein too much. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. The utility model provides a box for whole membrane is hatched, includes box body (1), one side of box body (1) articulates there is lid (2), its characterized in that: box body (1) is a cavity, the whole membrane of many antibodies has been placed in box body (1), box body (1) bottom both sides are equipped with arc slide (3), arc slide (3) sliding connection has slider (4), be equipped with locating hole (5) on slider (4) and the contact surface of box body (1) lateral wall, box body (1) lateral wall is equipped with spacing hole of a plurality of (6), be connected with locating pin (7) on box body (1) lateral wall, locating pin (7) pass spacing hole (6) and locating hole (5), be equipped with picture peg groove (8) in slider (4), baffle (9) have been placed in picture peg groove (8), baffle (9) top is equipped with the hold-down mechanism who makes baffle (9) and box body (1) closely laminate.

2. A cartridge for whole membrane incubation according to claim 1, wherein: hold-down mechanism includes first compress tightly pole (10) and second and compresses tightly pole (11), second compress tightly pole (11) top is located in first compress tightly pole (10), be connected with spring (12) between first compress tightly pole (10) and the second compress tightly pole (11), be equipped with screw hole (13) on slider (4), first compress tightly pole (10) rotate and be connected with bolt (14), bolt (14) and screw hole (13) threaded connection, second compress tightly pole (11) bottom be equipped with baffle (9) top assorted recess (15).

3. A cartridge for whole membrane incubation according to claim 1, wherein: the partition plate (9) is provided with a sealing strip (16) used for blocking liquid at the contact joint with the insert plate groove (8), and the sealing strip (16) is arranged at the contact joint of the partition plate (9) and the whole multi-antibody film.

4. A cassette for whole membrane incubation according to claim 2, wherein: a clamping groove matched with the first pressing rod (10) is formed in the box cover (2).

5. A cassette for whole membrane incubation according to claim 1, wherein: the box body (1) is made of transparent materials, and scales (17) used for measuring and displaying the distance between the partition boards (9) are arranged on the side wall of the box body (1).

6. A cassette for whole membrane incubation according to claim 1, wherein: the height of the arc-shaped slide way (3) is greater than the height of the liquid in the box body (1).