CN218596434U

CN218596434U - Prevent cell contamination's culture medium interpolation device

Info

Publication number: CN218596434U
Application number: CN202223060065.4U
Authority: CN
Inventors: 王建飞
Original assignee: Anmengde Pharmaceutical Technology Shanghai Co ltd
Current assignee: Aimeifei Biopharmaceutical Technology (Shanghai) Co.,Ltd.
Priority date: 2022-11-17
Filing date: 2022-11-17
Publication date: 2023-03-10
Anticipated expiration: 2032-11-17

Abstract

The utility model belongs to the technical field of cell culture equipment, and a prevent that cell pollution's culture agent adds device is disclosed, including lid, round hole, ring groove, the inner chamber fixedly connected with urceolus of lid top one side, the first mesh board of bottom fixedly connected with of urceolus inner chamber, the middle part fixedly connected with handle piece of interface surface, angle fixedly connected with dead lever such as the bottom annular of second mesh board. The utility model discloses an elastic spring, the interface, support the dog, the cooperation between round hole and the ring groove isotructure, make the device have the effect that control culture solution added, make the interface drive second mesh board, interior pole and dead lever downstream through pressing down the handle piece, make the culture solution will pass through second mesh board, ring groove and round hole and get into to support among the inner chamber of dog, thereby block that the culture solution gets into to culture apparatus in, thereby realized the effect that control culture solution added, practiced thrift the culture solution simultaneously.

Description

Prevent cell contamination's culture medium interpolation device

Technical Field

The utility model belongs to the technical field of cell culture equipment, specifically a prevent cell pollution's culture agent interpolation device.

Background

The culture medium is culture solution, and the main components of the culture solution are amino acid, glucose, inorganic salt, vitamins, trace elements and the like. In order to maintain a stable pH, the culture broth is typically provided with a pH buffer system, and a small amount of phenol red is often added as a pH indicator.

For example, utility model with patent number CN201793575U belongs to a cell culture solution adding device. The cover comprises a cover body, wherein an interface connected with a cell culture solution containing container is arranged on the cover body, and an air vent convenient for the cell culture solution to enter is further arranged on the cover body. The cell culture solution adding cover can enable culture solution to complete the liquid adding process in a relatively sealed environment during the liquid adding process, thereby effectively avoiding the pollution caused by the fact that liquid is exposed in air or contacts with bacteria-carrying objects during liquid adding, enabling the whole operation process to be in a sterile state, and ensuring a better cell culture effect. Furthermore, the utility model discloses operation process is simple and convenient, can accomplish repetitious or continuous liquid feeding, compares with the liquid feeding mode through the syringe absorption, convenient and fast, and the process is simplified.

The existing culture solution adding device for filling culture solution is characterized in that an opening of a bag of the culture solution filled with the culture solution is connected with an interface through an operator, so that the culture solution in the bag is directly fed into a cover body through the interface, the pollution of the culture solution due to air contact is prevented, and meanwhile, the culture solution is completely poured into the culture device due to the fact that the device is lack of a control structure for adding the culture solution, so that the culture solution is wasted, and therefore, the culture solution adding device for preventing cell pollution is provided, and the problem provided in the background art is solved.

SUMMERY OF THE UTILITY MODEL

For solving the problem that proposes among the above-mentioned background art, the utility model provides a prevent culture agent interpolation device of cell pollution, press down the handle piece and make the interface drive the second mesh board, interior pole and dead lever downstream, and then make and support the dog downstream, make the culture solution will pass through the second mesh board, ring groove and round hole enter into to supporting among the inner chamber of dog, final culture solution will enter into to the cell culture device of lid below through first mesh board, when need not when adding the culture solution to culture device, stop pressing down the handle piece this moment, because the effect of elastic spring elasticity, make the device reset, thereby make the inner chamber of urceolus seal the round hole, the bottom of fixed block will be sealed at the top of dog simultaneously, thereby block the culture solution and get into to culture device, make this structure have the advantage that control culture solution added.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a prevent cell pollution's culture medium interpolation device, includes lid, round hole, ring groove, the inner chamber fixedly connected with urceolus of lid top one side, the first mesh board of bottom fixedly connected with of urceolus inner chamber, the middle part fixedly connected with at first mesh board top has elastic spring and outer pole, the top swing joint of outer pole surface has to support the dog, the middle part fixedly connected with fixed block of urceolus inner chamber, the inner chamber swing joint of fixed block has the interface, the bottom fixedly connected with second mesh board of interface inner chamber, the interior pole of inner chamber fixedly connected with at second mesh board middle part, the middle part fixedly connected with handle piece of interface surface, angle fixedly connected with dead lever such as the bottom annular of second mesh board.

In the above technical scheme, preferably, the circular holes are annularly and equiangularly arranged at the top of the outer surface of the abutting block, the circular groove is arranged at the bottom of the inner cavity of the outer barrel and is located between the first mesh plate and the circular hole, and the diameter of the circular hole is smaller than the length of the circular groove in the vertical direction.

In the above technical scheme, preferably, the elastic spring is sleeved on the outer surface of the outer rod, the top of the elastic spring is fixedly connected with the middle part of the top of the inner cavity of the abutting block, and the outer surface of the abutting block is movably connected with the inner cavity of the outer cylinder.

In the above technical scheme, preferably, the bottom of the outer surface of the connector is movably connected with an inner cavity of the fixed block, the top of the inner cavity of the connector is threaded and is matched with a bag opening of a bagged culture solution, the bottom of the outer surface of the inner rod is movably connected with the inner cavity of the outer rod, and the diameter of the top of the abutting block is larger than that of the bottom of the fixed block.

In the above technical scheme, preferably, the top of the handle block extends to the outside of the outer cylinder, and the bottom of the fixing rod abuts against the top of the stop block to be fixedly connected.

In the above technical solution, preferably, the elastic force of the elastic spring is greater than the gravity of the bagged culture solution.

In the above technical solution, preferably, the bottom of the inner cavity of the cover body is threaded and adapted to the cell culture device, and an opening is fixedly connected to one side of the top of the cover body away from the outer cylinder.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model discloses an elastic spring, the interface, support the dog, cooperation between round hole and the ring groove isotructure, make the device have the effect that control culture solution added, make the interface drive the second mesh board through pressing down the handle piece, interior pole and dead lever downstream, and then make and support the dog and move down, make the culture solution will pass through the second mesh board, ring groove and round hole enter into to support among the inner chamber of dog, final culture solution will enter into to the cell culture device of lid below through first mesh board, when need not add the culture solution to culture device, stop pressing down the handle piece this moment, because the effect of elastic spring elasticity, make the device reset, thereby make the inner chamber of urceolus seal the round hole, support the bottom that the top of dog will seal the fixed block simultaneously, thereby stop the culture solution to get into culture device, thereby the effect that control culture solution added has been realized, the culture solution has been practiced thrift simultaneously.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the front cross-section structure of the present invention;

FIG. 3 is an enlarged view taken at A in FIG. 2;

fig. 4 is an exploded view of the stopping block of the present invention.

In the figure: 1. a cover body; 2. an outer cylinder; 3. a first mesh plate; 4. an elastic spring; 5. an outer rod; 6. a stop block; 7. a fixed block; 8. an inner rod; 9. an interface; 10. a second mesh plate; 11. a handle block; 12. fixing the rod; 13. a circular hole; 14. a circular groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1 to 4, the utility model provides a cell pollution preventing culture medium adding device, which comprises a cover body 1, a circular hole 13 and a circular groove 14, wherein an outer barrel 2 is fixedly connected to an inner cavity on one side of the top of the cover body 1, a first mesh plate 3 is fixedly connected to the bottom of the inner cavity of the outer barrel 2, an elastic spring 4 and an outer rod 5 are fixedly connected to the middle of the top of the first mesh plate 3, a resisting block 6 is movably connected to the top of the outer surface of the outer rod 5, a fixed block 7 is fixedly connected to the middle of the inner cavity of the outer barrel 2, an interface 9 is movably connected to the inner cavity of the fixed block 7, a second mesh plate 10 is fixedly connected to the bottom of the inner cavity of the interface 9, an inner rod 8 is fixedly connected to the inner cavity of the middle of the second mesh plate 10, a handle block 11 is fixedly connected to the middle of the outer surface of the interface 9, and a fixed rod 12 is fixedly connected to the bottom of the second mesh plate 10 at equal angles; make interface 9 drive second mesh board 10 through pressing down handle piece 11, interior pole 8 and dead lever 12 lapse, and then make to stop 6 lapse, make the culture solution will pass through second mesh board 10, ring groove 14 and round hole 13 enter into to stop 6's inner chamber among, final culture solution will enter into to the cell culture device of lid 1 below through first mesh board 3, when need not add the culture solution to culture device in, stop pressing down handle piece 11 this moment, because the effect of elastic spring 4 elasticity, make the device reset, thereby make the inner chamber of urceolus 2 seal round hole 13, the bottom of fixed block 7 will be sealed at the top of stopping 6 simultaneously, thereby stop that the culture solution gets into to culture device.

As shown in fig. 3 and 4, the circular hole 13 is annularly arranged at the top of the outer surface of the resisting block 6 at an equal angle, the circular groove 14 is arranged at the bottom of the inner cavity of the outer barrel 2 and is positioned between the first mesh plate 3 and the circular hole 13, the diameter of the circular hole 13 is smaller than the length of the circular groove 14 in the vertical direction, the elastic spring 4 is sleeved on the outer surface of the outer rod 5, the top of the elastic spring 4 is fixedly connected with the middle part of the top of the inner cavity of the resisting block 6, and the outer surface of the resisting block 6 is movably connected with the inner cavity of the outer barrel 2; move down through resisting dog 6 to make round hole 13 and ring groove 14 be linked together, and then make the culture solution enter into to resisting among dog 6's the inner chamber through ring groove 14 and round hole 13, the culture solution passes through first mesh plate 3 afterwards and enters into among the cell culture device of urceolus 2 below.

As shown in fig. 2 and 3, the bottom of the outer surface of the interface 9 is movably connected with the inner cavity of the fixed block 7, the top of the inner cavity of the interface 9 is threaded and is matched with the bag opening of the bagged culture solution, the bottom of the outer surface of the inner rod 8 is movably connected with the inner cavity of the outer rod 5, the diameter of the top of the resisting block 6 is larger than that of the bottom of the fixed block 7, the top of the handle block 11 extends to the outer side of the outer cylinder 2, the bottom of the fixed rod 12 is fixedly connected with the top of the resisting block 6, and the elasticity of the elastic spring 4 is larger than the gravity of the bagged culture solution; through the effect of 4 elasticity of elastic spring, when operating personnel stopped to press handle piece 11, will make this moment to support dog 6 to drive dead lever 12 and second mesh board 10 rebound, make interior pole 8, interface 9 and culture solution upward movement in bags simultaneously, when the top that supports dog 6 contacts with the bottom of fixed block 7, support dog 6 and stop moving, the device resets to in the continuation of operating personnel is to adding the culture solution in the cell culture device next time.

As shown in fig. 1 and 2, the bottom of the inner cavity of the cover body 1 is in a thread shape and is matched with a cell culture device, and one side of the top of the cover body 1, which is far away from the outer cylinder 2, is fixedly connected with an opening; through the screw thread design of lid 1 bottom to be convenient for operating personnel to install the device on the cell culture device, open-ended design is convenient for adding the cell that needs to cultivate among the operating personnel looks cell culture device simultaneously.

The utility model discloses a theory of operation and use flow:

firstly, the bagged culture solution is screwed into the inner cavity at the top of the interface 9 by an operator, when the operator needs to add the culture solution into the cell culture device, the handle block 11 is pressed downwards at the moment, the interface 9 can drive the second mesh plate 10, the inner rod 8 and the fixing rod 12 move downwards, and then the abutting block 6 moves downwards, at the moment, the elastic spring 4 is in a compression state, meanwhile, the cell solution can flow out of the inner cavity of the interface 9 from the bagged culture solution, meanwhile, the culture solution passes through the second mesh plate 10 and flows into the inner cavities of the outer cylinders 2 below the

fixed blocks

7 and 7, at the moment, the culture solution can subsequently enter the inner cavity of the abutting block 6 through the circular groove 14 and the circular hole 13, finally, the culture solution can enter the cell culture device through the first mesh plate 3, when the culture solution does not need to be added into the culture device, at the moment, the operator stops pressing the handle block 11, due to the elastic force effect of the elastic spring 4, the device is reset, so that the inner cavity of the outer cylinder 2 seals the circular hole 13, and the bottom of the fixed block 6 is sealed at the top of the abutting block 6, and the bottom of the culture solution can enter the culture device.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a prevent cell contamination's culture medium interpolation device, includes lid (1), round hole (13), ring groove (14), its characterized in that: inner chamber fixedly connected with urceolus (2) of lid (1) top one side, the first mesh board of bottom fixedly connected with (3) of urceolus (2) inner chamber, the middle part fixedly connected with at first mesh board (3) top has elastic spring (4) and outer pole (5), the top swing joint of outer pole (5) surface has to dog (6), the middle part fixedly connected with fixed block (7) of urceolus (2) inner chamber, the inner chamber swing joint of fixed block (7) has interface (9), the bottom fixedly connected with second mesh board (10) of interface (9) inner chamber, pole (8) in the inner chamber fixedly connected with at second mesh board (10) middle part, the middle part fixedly connected with handle piece (11) of interface (9) surface, angle fixedly connected with dead lever (12) such as the bottom annular of second mesh board (10).

2. The device for adding culture medium for preventing cell contamination according to claim 1, wherein: round hole (13) annular is seted up at the top of resisting dog (6) surface of equidistance, ring groove (14) are seted up in the bottom of urceolus (2) inner chamber and are located between first mesh board (3) and round hole (13), the diameter of round hole (13) is less than the length of ring groove (14) vertical direction.

3. The cell contamination preventive culture medium adding device according to claim 1, wherein: the elastic spring (4) is sleeved on the outer surface of the outer rod (5), the top of the elastic spring (4) is fixedly connected with the middle of the top of the inner cavity of the abutting block (6), and the outer surface of the abutting block (6) is movably connected with the inner cavity of the outer barrel (2).

4. The cell contamination preventive culture medium adding device according to claim 1, wherein: the bottom of the outer surface of the connector (9) is movably connected with an inner cavity of the fixing block (7), the top of the inner cavity of the connector (9) is in a threaded shape and is matched with a bag opening of a bagged culture solution, the bottom of the outer surface of the inner rod (8) is movably connected with the inner cavity of the outer rod (5), and the diameter of the top of the abutting block (6) is larger than that of the bottom of the fixing block (7).

5. The cell contamination preventive culture medium adding device according to claim 1, wherein: the top of the handle block (11) extends to the outer side of the outer barrel (2), and the bottom of the fixed rod (12) is fixedly connected with the top of the stop block (6).

6. The device for adding culture medium for preventing cell contamination according to claim 1, wherein: the elasticity of the elastic spring (4) is larger than the gravity of the bagged culture solution.

7. The device for adding culture medium for preventing cell contamination according to claim 1, wherein: the bottom of the inner cavity of the cover body (1) is in a thread shape and is matched with a cell culture device, and one side, far away from the outer barrel (2), of the top of the cover body (1) is fixedly connected with an opening.