CN218589182U - Device for fixing mouse - Google Patents

Abstract

The utility model discloses a device of fixed mouse relates to animal medical treatment experiment technical field, provides a mouse fixer of two photon microscope observation experiments usefulness is carried out mouse head side position, and the device includes: the device can be adjusted by 360 degrees in the vertical and horizontal directions, and is also provided with a blunt tip-shaped fixer which can symmetrically fix temporal bones of the mouse in the horizontal and vertical positions; the body bearing seat is arranged on the base, consists of a bearing platform and a binding band and can rotate 90 degrees in the horizontal direction. The utility model discloses mouse fixer not only can fix the head and the health of mouse simultaneously, still can realize fixing the side position of mouse, not only can satisfy the function of general mouse fixer, still can realize exposing mouse side position jaw, is convenient for carry out the micro-imaging observation experiment of two-photon and other operation operations to side position jaw.

Description

Device for fixing mouse

Technical Field

The utility model relates to an animal medical treatment experiment technical field especially relates to the fixed technical field of mouse, especially relates to a mouse fixer of two-photon microscope observation experiment usefulness is carried out mouse head side position.

Background

Animal experiment research is one of important experimental contents in the field of medical research, and development of in vivo animal experiments in the fields of basic medicine and biology has become one of important research ways for solving specific scientific problems.

With the development of microscopic imaging technology in recent years, the two-photon microscopic imaging technology is a new imaging technology combining a laser scanning confocal microscope and a two-photon excitation technology, and because the technology has deep imaging depth and low phototoxicity, is suitable for observing living cells and imaging animal carriers, and is widely used in the field of neurobiology at present for observing the activity of neurons of living animals, and more researchers also apply the technology to the in-vivo activity process of the living animals and the cells.

However, the most difficult point when using a two-photon microscope to observe living animals is the fixation of mice, especially the fixation of mice under non-anesthesia state, because the requirement for the fixation state of mice is high during the shooting process, and the requirement for the distance between the objective lens and the observation area is short, and in addition, because the observation needs to be performed by a water lens during the two-photon microscopic imaging, the observation area needs to be relatively horizontal and can hold a drop of water. The fixation requirement on the mouse is very high when the two-photon microscopic observation is carried out, and no device which can be used for carrying out the two-photon microscopic observation on the fixation of the mouse body and the lateral jaw bone exists at present.

Disclosure of Invention

To the problem that exists, the utility model discloses an it can't satisfy the mouse side position and fix and carry out the not enough that two photon microscope observes to overcome current device of fixed mouse, provides a device of fixed mouse, the utility model discloses mouse fixing device not only can fix the head and the health of mouse simultaneously, still can realize fixing the side position of mouse, not only can satisfy the function of general mouse fixer, still can realize exposing mouse side position jawbone, is convenient for carry out two photon microscopic imaging observation experiment and other operation to side position jawbone.

The utility model discloses the main thinking of the technical scheme who adopts: a device for fixing a mouse consists of a base, a head fixing seat and a body bearing seat; the head fixing seat is arranged on the base, is provided with a coupling device which can hook the upper incisors and the adjustable upper jaw of the mouse, the horizontal distance between the devices for fixing the upper incisors and the upper jaw of the mouse can be adjusted, the head fixing seat can be adjusted by 360 degrees in the vertical direction and the horizontal direction, and is also provided with a blunt tip-shaped fixer which can symmetrically fix the temporal bones of the mouse in the horizontal position and the vertical position; the body bearing seat is arranged on the base and consists of a bearing platform and a binding band, and the body bearing seat can rotate 90 degrees in the horizontal direction.

In order to realize the above object, the utility model provides a carry out the mouse fixer that two-photon microscope observation experiments were used, a device of fixed mouse, its characterized in that to the fixed of mouse health and side position jawbone: the head-carrying type massage device comprises a base, a head fixing seat and a body carrying seat, wherein the head fixing seat and the body carrying seat are both arranged on the upper surface of the base;

the head fixing seat comprises an upper jaw fixer and temporal bone fixers, and the temporal bone fixers are symmetrically distributed on the left side and the right side of the upper jaw fixer;

a sliding assembly is arranged at the part of the temporal bone fixator connected with the base;

the temporal bone fixator comprises a temporal bone support column and a pause needle, and an adjusting device is arranged at the joint of the pause needle and the temporal bone support column;

the health bears the seat and includes flat board, health support column and sets up the ring at health support column both ends, has seted up the ring track on the ring, dull and stereotyped and ring track swing joint it is right to set on the ring track the dull and stereotyped braking component that rotates spacingly that carries out, on braking component was located to the flat board, dull and stereotyped top was equipped with the bandage that is used for fixed mouse health.

Furthermore, the maxilla fixer includes main tributary support column, incisor hook and upper jaw stationary dog all with screw fixed connection, and the screw passes the hole of seting up on the main tributary support column.

Furthermore, a spiral structure for adjusting the vertical distance between the incisor hook and the upper jaw fixing clamp is arranged between the incisor hook and the upper jaw fixing clamp.

Furthermore, the adjusting device comprises a screw assembly A, a screw assembly B, a first adjusting plate and a second adjusting plate, wherein a sliding groove is formed in the first adjusting plate, and the angle of the first adjusting plate relative to the temporal bone supporting column is adjusted through the screw assembly B and the sliding groove; the adjusting plate II is movably connected with the adjusting plate I through a screw component A.

Furthermore, the screw component A, the screw component B and the brake component respectively comprise a screw and a nut, and the screw and the nut are arranged in a matched mode.

Furthermore, the circular ring is fixedly connected with the circular ring track through a fixing strip, and the circular ring is fixedly connected with the body supporting column through an L-shaped fixing clamp.

The beneficial effects of the utility model are that: compared with the prior art, the utility model discloses an improve the part and lie in:

1. the head fixing base can be adjusted by 360 degrees in the vertical and horizontal directions, the head fixing base comprises a temporal bone fixing device besides a device for fixing the upper jaw of the mouse, the head fixing base and the device are matched to fix the head of the mouse, and the directions of the upper jaw fixing device and the temporal bone fixing device of the mouse can be adjusted, so that the utility model discloses a mouse fixing device not only can meet the function of a common mouse fixer, but also can realize horizontal exposure of the lateral jaw of the mouse, stabilize and fix the lateral position of the mouse, and is convenient for carrying out two-photon microscopic imaging observation experiments and other operation operations on the lateral jaw position;

2. the temporal bone fixator is provided with two symmetrical support columns and two temporal bone fixing blunt needles, and the blunt needle head can be provided with a rubber sleeve; it is sectional type design to pause the needle, and the length of pausing the needle is adjustable, in addition, but pause needle and support column coupling part and also set up the device that the connection position vertical direction adjusted, the device is adjusted through screw subassembly A's rotation and the removal of spout on screw subassembly B is adjusted, can realize two temples fixed needle and to the fixed of mouse temples bone in the vertical direction, through fixed mouse temples bone behind the maxillary fixator mouse head simultaneously, because this fixed head device direction is adjustable, consequently, can realize the fixed to mouse side position head, expose the side level of mouse.

3. The body bearing seat comprises a body supporting column, a flat plate, a circular ring and a bandage, wherein a circular ring track is arranged on the circular ring, the circular ring track is fixedly connected with the circular ring through a fixing strip, the fixing strip is a long-strip-shaped nylon fixing strip, the circular ring is bound with the side edge of the circular ring track to achieve the effect of fixing the circular ring track and the circular ring, the circular ring is fixedly connected with the body supporting column through an L-shaped fixing clamp, the L-shaped fixing clamp is limited through a screw, the circular ring is clamped between two L-shaped fixing clamps, the two fixing clamps are fixedly penetrated through by the screw, the circular ring is fixed between the two L-shaped fixing clamps, 1 body supporting column is fixedly connected with the circular ring through 2 pairs of L-shaped fixing clamps, the connection mode can enable a hand-screwed screw on the circular ring track to slide on the circular ring without being blocked, the flat plate is arranged between the two circular rings, a braking fixing component is arranged on the circular ring track, the flat plate is fixedly connected with the braking fixing component, the hand-screwed screw in the braking fixing component can slide along the circular ring track, so that the included angle between the flat plate and the circular ring track can be adjusted, and the circular ring track, and a nut can be fixed by the hand-screwed nut.

Drawings

Fig. 1 is a schematic view of the overall structure of the mouse fixing device of the present invention.

Fig. 2 is an enlarged view of the body support base.

Fig. 3 is an enlarged view of the body support after the plate is rotated by a certain angle.

Fig. 4 is an enlarged view of the main support column and the maxillary fixator.

Fig. 5 is a side view of the connection part of the main support column and the maxillary fixator.

Figure 6 is a side view of the connection of the ring to the body support post.

Fig. 7 is a schematic view of an adjusting device of the connecting part of the blunt needle and the supporting column.

Fig. 8 is a schematic diagram of a blunt needle.

Wherein: 1. the device comprises a main supporting column, 2, screws, 211, a matched nut, 3, a screw device, 4, an incisor hook, 5, upper jaw fixing forceps, 6, a blunt needle, 8, a temporal bone supporting column, 9, a body supporting column, 10, a circular ring, 101, a hand-screwed screw, 102, a circular ring track, 103, a nut, 104, an L-shaped fixing clamp, 105, a fixing strip, 11, a bandage, 12, a flat plate, 13, a base, 15, a sliding rail, 151, a sliding block, 25, screw components A,26, screw components B,27, a sliding groove, 22, a first adjusting plate, 23, a second adjusting plate, 24 and an adjusting device.

Detailed Description

In order to make those skilled in the art better understand the technical solution of the present invention, the following describes the technical solution of the present invention with reference to the embodiments and the accompanying drawings.

The utility model discloses a mouse fixing device includes base 13, the head fixing base, the health bears the seat, please refer to fig. 1-8, the head of mouse is fixed to the head fixing base, this fixing base is including the main tributary dagger 1 that plays bearing and connection effect and the device of fixed mouse upper jaw, the device is including the incisor hook 4 that can hook the incisor on the mouse and the upper jaw stationary tong 5 of fixed mouse upper jaw, incisor hook 4 and upper jaw stationary tong 5 all adopt elastic spring steel board as raw materials, can realize adjusting the distance between incisor hook 4 and the upper jaw stationary tong 5 like this, incisor hook and upper jaw pincers are fixed into an organic whole through screw means 3 that can adjust the vertical distance between the two, the terminal welding of this whole is in the same place, and this whole terminal and main tributary dagger 1 clearing hole are fixed with screw 2 that can manually rotate to link to each other, can realize incisor hook and upper jaw pincers 360 rotations through the rotation of screw 2, the horizontal direction also can move back and forth simultaneously; after the screw 2 is rotated to a length and an angle required by a user, the screw can be fixed by the nut 211, so that the maxillary fixator can be maintained in a stable state.

The head fixing seat comprises a temporal bone fixing device besides a device for fixing the upper jaw of the mouse, the device consists of two symmetrical support columns 8 and two temporal bone fixing blunt needles 6, and the heads of the blunt needles 6 can be provided with rubber sleeves; the blunt needle 6 is designed in a sectional mode, the length of the blunt needle is adjustable, an adjusting device is vertically arranged at the connecting part of the blunt needle and a temporal bone support column, the blunt needle is movably connected with a screw assembly A25 and a first adjusting plate 22 through a second adjusting plate 23, the second adjusting plate 23 is an arc-shaped plate and can clamp the blunt needle 6 to prevent the blunt needle from falling off, the device can fix the temporal bones of the mouse in the vertical direction through the rotation of the screw assembly A25 and a screw assembly B26, the length of the first adjusting plate 22 can be adjusted through a sliding groove 27, the temporal bones of the mouse can be fixed while the upper jaw of the mouse is fixed, the lateral head of the mouse can be fixed, and the lateral surface of the mouse is horizontally exposed;

the body bearing seat consists of four support columns 9, a flat plate 12, two circular rings 10 and a bandage 11, wherein the flat plate 12 is arranged between the two circular rings 10, circular ring tracks 102 are arranged on the circular rings 10, braking components are arranged on the circular ring tracks 102 and comprise hand-screwed screws 101 and nuts 103, the flat plate 12 is fixed on the braking components, the braking components comprise at least 4 hand-screwed screws 101, the hand-screwed screws 101 penetrate through the circular ring tracks 102 and slide along the circular ring tracks 102 to adjust the horizontal included angle of the flat plate 12, and the nuts 103 are used for fixing the hand-screwed screws 101 after the horizontal included angle is adjusted to a required angle; the ring 10 is fixedly connected with the ring track 102 through a fixing strip 105, the fixing strip 105 is a strip-shaped nylon fixing strip, the ring track 102 and the ring 10 are fixed by binding the side edges of the ring 10 and the ring track 102 together, the ring 10 is fixedly connected with the body support column 9 through an L-shaped fixing clamp 104, the L-shaped fixing clamp 104 is limited through a screw, the ring is clamped between the two L-shaped fixing clamps 104, the two fixing clamps are penetrated and fixed through by a screw, so that the ring is fixed between the two L-shaped fixing clamps 104, the hand-screwed screw 101 on the ring track 102 can slide on the ring 10 without being blocked in the connection mode, the bandage 11 is positioned on the flat plate 12 and between the rings 10, and can be used for fixing the body of a mouse, so that the mouse lies prostrate and is tightly attached to the flat plate;

through adjusting the head fixing base, the direction and the position that the health bore the seat can be fixed the head and the health of mouse simultaneously, not only can satisfy the function of general mouse fixer, still can realize the level and expose mouse mandible, the side position of fixed mouse is convenient for carry out the micro-imaging observation experiment of two-photon and other operation operations to side position jaw position.

Examples

In the embodiment, the body bearing seat is used for fixing the trunk of a mouse, the body of the mouse is placed on the flat plate 12, the body of the mouse is fixed by the bandage 11, the mouse is tightly attached to the flat plate 12 in the prone position, the hand-screwed screw 101 can slide along the circular ring track 102 to adjust the horizontal included angle of the flat plate, the purpose of adjusting the body angle of the mouse is further achieved, the hand-screwed screw 101 is fixed by the nut 103 after the angle required by a user is adjusted, and the head of the mouse is fixed by the head fixing seat after the body of the mouse is fixed; firstly, an incisor hook 4 in a device for fixing the upper jaw of a mouse is used for hooking the upper incisor of the mouse, an upper jaw fixing clamp 5 for fixing the upper jaw of the mouse is used for fixing the upper jaw of the mouse, the incisor hook 4 and the upper jaw fixing clamp 5 are made of elastic spring steel plates, so that the vertical distance between the incisor hook 4 and the upper jaw fixing clamp 5 can be adjusted through a screw device 3, meanwhile, because the incisor hook 4 and the tail end of the upper jaw fixing clamp 5 are welded and fixed into a whole, the tail end of the whole is fixedly connected with a main supporting column 1 through a hole and a screw 2 which can be manually rotated, the upper jaw fixing device can rotate for 360 degrees through the rotation of the screw 2, meanwhile, the horizontal direction can also move back and forth, a temporal bone fixing needle in the temporal bone fixing device is used for fixing the temporal bone of the mouse, the temporal bone needle 6 is designed in a sectional type, the length can be adjusted, and the fixation of the two temporal bone fixing needles can be fixed in the vertical direction through the rotation adjustment of a screw component A25 and the movement adjustment of a sliding groove 27 on a screw component B26; the body bears the weight of the seat and the two cooperation realization of head fixing base to the fixed needs in order to satisfy two-photon microscope observation of mouse health and side position jaw.

The foregoing shows and describes the basic principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only illustrative of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the present invention, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (6)

1. A device for immobilizing a mouse, comprising: the head and body carrying seat is arranged on the upper surface of the base (13);

the head fixing seat comprises an upper jaw fixer and temporal bone fixers, and the temporal bone fixers are symmetrically distributed on the left side and the right side of the upper jaw fixer;

the part of the temporal bone fixator connected with the base (13) is provided with a sliding assembly;

the temporal bone fixator comprises a temporal bone support column (8) and a pause needle (6), and an adjusting device (24) is arranged at the joint of the pause needle (6) and the temporal bone support column (8);

the health bears the seat and includes dull and stereotyped (12), health support column (9) and sets up ring (10) at health support column both ends, has seted up ring track (102) on ring (10), dull and stereotyped (12) and ring track (102) swing joint set on ring track (102) right dull and stereotyped (12) carry out rotatory spacing brake assembly, on brake assembly was located in dull and stereotyped (12), dull and stereotyped (12) top was equipped with bandage (11) that are used for fixed mouse health.

2. The apparatus for immobilizing a mouse according to claim 1, wherein: the upper jaw fixer comprises a main support column (1), an incisor hook (4) and upper jaw fixing forceps (5), the incisor hook (4) and the upper jaw fixing forceps (5) are fixedly connected with a screw (2), and the screw (2) penetrates through a hole formed in the main support column (1).

3. The apparatus for immobilizing a mouse according to claim 2, wherein: a spiral structure (3) for adjusting the vertical distance between the incisor hook (4) and the upper jaw fixing forceps (5) is arranged between the incisor hook and the upper jaw fixing forceps.

4. The apparatus for immobilizing a mouse according to claim 1, wherein: the adjusting device (24) comprises a screw assembly A (25), a screw assembly B (26), a first adjusting plate (22) and a second adjusting plate (23), a sliding groove is formed in the first adjusting plate (22), and the angle of the first adjusting plate (22) relative to the temporal bone supporting column (8) is adjusted through the screw assembly B (26) and the sliding groove (27); the second adjusting plate (23) is movably connected with the first adjusting plate (22) through a screw component A (25).

5. The apparatus for immobilizing a mouse according to claim 4, wherein: the screw component A (25), the screw component B (26) and the brake component all comprise screws and nuts, and the screws and the nuts are arranged in a matched mode.

6. The apparatus for immobilizing a mouse according to claim 1, wherein: the ring (10) is fixedly connected with the ring track (102) through a fixing strip (105), and the ring (10) is fixedly connected with the body supporting column (9) through an L-shaped fixing clamp (104).

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