CN218516771U - Low-temperature ultra-clean operating platform for preparing 3D cell culture glue drops - Google Patents
Low-temperature ultra-clean operating platform for preparing 3D cell culture glue drops Download PDFInfo
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- CN218516771U CN218516771U CN202222366042.XU CN202222366042U CN218516771U CN 218516771 U CN218516771 U CN 218516771U CN 202222366042 U CN202222366042 U CN 202222366042U CN 218516771 U CN218516771 U CN 218516771U
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Abstract
The utility model belongs to the technical field of 3D cell culture, a preparation 3D cell culture glues low temperature superclean bench of dripping is disclosed, including the operation cabinet, one or more compressor, one or more are installed to the bottom of operation cabinet the compressor with operation cabinet electric connection, the operation is on cabinet and be located one or more the top of compressor is provided with the operation panel, the rear side of operation panel is equipped with light, ultraviolet lamp and cold wind board, the light with the ultraviolet lamp is installed on the operation cabinet and with operation cabinet electric connection, the front side of operation panel is equipped with the glass window, cold wind board fixed mounting be in on the operation cabinet and seted up a plurality of cold wind holes, the utility model discloses a set up circulation refrigeration, can make operation panel mesa and surrounding environment reach 4 ℃, can effectively guarantee the low temperature environment among the matrix glue operation process, overcome the inconvenience of using the ice chest operation, have beneficial effect such as convenience, clean.
Description
Technical Field
The utility model relates to a 3D cell culture technical field especially relates to a preparation 3D cell culture glue drips low temperature superclean bench.
Background
Organoids (Organoids) refer to tissue analogs having a certain spatial structure formed by in vitro three-dimensional (3D) culture using adult stem cells or pluripotent stem cells, which can maximally mimic in vivo tissue structure and function and can be stably subcultured for a long period of time. Compared with the traditional two-dimensional culture model, the method has the advantages of being closer to the composition and behavior of physiological cells, having more stable genome, being more suitable for biological transfection and high-throughput screening and the like. Compared with animal models, organoid models are simpler to operate and can also be used for researching mechanisms such as disease occurrence and development. Therefore, the method has wide application prospects in the fields of organ development, precise medical treatment, regenerative medicine, drug screening, gene editing, disease modeling and the like. In 2013, organoids were rated by the journal of science as ten years of technology. In the early 2018, the organoids are rated as "nature method" as the 2017 method.
Although organoids have very broad application prospects in basic research and drug screening, relevant organoid standards are lacking. An important part of organoid culture is to mix cells with matrigel, and then drop the mixture into a culture well plate in the form of gel drops for three-dimensional culture, and the matrigel can maintain a liquid state at 4 degrees and gradually solidifies once placed at room temperature, so at present, operations involving matrigel need to be performed on ice, an ice box needs to be placed in an operation table, and many operation inconvenience and operation steps are caused (for example, a suction head needs to be precooled in PBS on ice before gel suction every time). Meanwhile, the 3d cell glue dispensing can be carried out by utilizing automatic equipment in the future, and the existing superclean workbench does not have a refrigerating function and cannot be used for conveniently interconnecting equipment.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a preparation 3D cell culture glue drips cryogenic ultra-clean operation panel to improve the convenience of the operation that relates to matrigel in 3D organoid cultivates.
In order to realize the purpose of the utility model, the adopted technical proposal is that: the utility model discloses a preparation 3D cell culture glue drips low temperature superclean bench, including the operation cabinet, one or more compressor, one or more are installed to the bottom of operation cabinet the compressor with operation cabinet electric connection, the operation is cashier's office in a shop and is located one or more the top of compressor is provided with the operation panel, the rear side of operation panel is equipped with light, ultraviolet lamp and cold wind board, the light with the ultraviolet lamp is installed the operation is cashier's office in a shop and with operation cabinet electric connection, the front side of operation panel is equipped with the glass window, cold wind board fixed mounting be in the operation is cashier's office in a shop and seted up a plurality of cold wind holes.
The utility model discloses further set up to: one or more sockets are also arranged on the rear side of the operating platform.
The utility model discloses further set up to: the left and right sides of operation panel all is equipped with the closing plate, the closing plate fixed mounting be in on the operation cabinet.
The utility model discloses further set up to: and a control panel is arranged below the operating platform and is fixedly arranged on the operating cabinet.
The utility model discloses further set up to: one side of the control panel is provided with a plurality of heat dissipation holes, and the heat dissipation holes are formed in the operation cabinet.
The utility model discloses further set up to: a plurality of movable wheels are installed at the bottom of the operation cabinet.
The utility model discloses further set up to: a closed cavity is arranged above the operating table, and sealing partition plates are arranged on the left side and the right side of the closed cavity.
The utility model discloses further set up to: the operation panel is made of stainless steel materials.
The utility model discloses further set up to: the sealing plate is made of a glass material.
The utility model discloses further set up to: the length, width and height of the operation cabinet are 1200mm, 800mm and 1600mm respectively.
To sum up, compare with prior art, the utility model discloses a preparation 3D cell culture glues low temperature superclean bench of dripping, the utility model discloses a set up circulation refrigeration, can make operation panel mesa and surrounding environment reach 4 ℃, can effectively guarantee the low temperature environment of matrix glue operation in-process, overcome the inconvenience of using the ice chest operation, have beneficial effect such as convenient, clean.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a low-temperature ultra-clean operating platform for preparing 3D cell culture gel drops according to this embodiment;
FIG. 2 is a schematic sectional view of a first cryo-super clean bench for preparing 3D cell culture gel drops according to this embodiment;
fig. 3 is a schematic cross-sectional view of a second cryo-super clean bench for preparing 3D cell culture gel drops according to this embodiment.
Reference numerals are as follows: 1. an operation cabinet; 2. a compressor; 3. an operation table; 4. an illuminating lamp; 5. an ultraviolet lamp; 6. a cold air plate; 7. a glass window; 8. a cold air hole; 9. a socket; 10. sealing plates; 11. a control panel; 12. heat dissipation holes; 13. a moving wheel; 14. sealing the chamber; 15. the separator is sealed.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments, it is to be understood that the specific embodiments described herein are only used for explaining the present invention, and are not used for limiting the present invention.
In the description of the present invention, it should be noted that the terms "front", "back", "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, or may be communicated with each other inside the two elements, or may be wirelessly connected or wired connected. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Furthermore, the technical features mentioned in the different embodiments of the present invention described above can be combined with each other as long as they do not conflict with each other.
In one aspect of the present embodiment, as shown in fig. 1, 2 and 3, there is provided a low temperature ultra-clean operating platform for preparing 3D cell culture gel drops, comprising an operating cabinet 1, one or more compressors 2 installed at the bottom of the operating cabinet 1, one or more compressors 2 electrically connected to the operating cabinet 1, an operating platform 3 installed on the operating cabinet 1 and above the one or more compressors 2, an illuminating lamp 4, an ultraviolet lamp 5 and a cold air plate 6 installed at the rear side of the operating platform 3, the illuminating lamp 4 and the ultraviolet lamp 5 installed on the operating cabinet 1 and electrically connected to the operating cabinet 1, a glass window 7 installed at the front side of the operating platform 3, and the cold air plate 6 fixedly installed on the operating cabinet 1 and provided with a plurality of cold air holes 8, in the present embodiment, the number of compressors 2 is preferably two, that is a refrigeration system with two compressors 2 is adopted, when one of the compressors 2 fails, the other compressor can continue to work, so as to ensure safety, the number of the glass windows 7 is preferably two, one of the compressors is fixedly installed on the operating cabinet 1, and another compressor is slidably connected to the operating platform, so as to be capable of freely pushing the compressor 3, and enabling the operation of the cold air to be freely performed, and to obtain a low temperature of the operating platform 3, and to be capable of freely surrounding the operation condition of being obtained.
Further, one or more sockets 9 are further disposed at the rear side of the console 3, and in this embodiment, the number of the sockets 9 is preferably two, and the sockets are used for electrically connecting the automated dispensing equipment and the like with the operation cabinet 1.
Further, the left and right sides of operation panel 3 all is equipped with sealing plate 10, and sealing plate 10 fixed mounting is on operation cabinet 1, and in this embodiment, sealing plate 10 is made by glass material, and in the concrete implementation in-process, sealing plate 10 made by glass material not only can provide sealed operational environment for operation panel 3, and its transparent state still is convenient for observe operation panel 3 in real time moreover, has improved the reliability of its work.
Further, a control panel 11 is arranged below the operation table 3, the control panel 11 is fixedly installed on the operation cabinet 1, and in the specific implementation process, an operator can control and adjust the operation of each component on the operation cabinet 1 through the control panel 11.
Further, one side of control panel 11 is equipped with a plurality of louvres 12, and a plurality of louvres 12 are seted up on operation cabinet 1, and at the concrete implementation in-process, louvre 12 can in time discharge operation cabinet 1 with the heat of compressor 2 in the production of refrigeration in-process to ensure the sustainable work of this equipment.
Further, a plurality of moving wheels 13 are installed at the bottom of the operation cabinet 1, in this embodiment, the number of the moving wheels 13 is preferably four, and the moving wheels 13 are respectively arranged at four corners of the bottom of the operation cabinet 1 to facilitate the movement of the device.
In the specific implementation process, a closed chamber 14 is arranged above the operating platform 3, and sealing partition plates 15 are mounted on the left side and the right side of the closed chamber 14.
Further, the console 3 is made of stainless steel, and it should be noted that the console 3 made of stainless steel has the advantages of corrosion resistance, high hardness, and the like, and in the specific implementation process, the console 3 may also be made of other high-quality materials.
In the implementation process, the length, the width and the height of the operation cabinet 1 are respectively 1200mm, 800mm and 1600mm.
In another aspect of this embodiment, two use cases of the present device are provided (both bladder cancer organoids are exemplified).
Case one:
1) Starting up and precooling the operation table 3;
2) When the operation table 3 is lowered to 4 ℃, the glass window 7 is pulled down to seal the operation table 3, reagent consumables needing precooling, such as a liquid transfer suction head, are placed in the operation table 3, ultraviolet disinfection is carried out for 15 minutes, and the ultraviolet lamp 5 is turned off;
3) Placing matrigel thawed in a refrigerator at 4 ℃ on the table top of an operation table 3 for later use;
4) Cells formed by treating tumor tissues are directly and uniformly mixed with matrigel in an operation table 3, and the cells are dispensed into a culture pore plate;
5) And placing the culture plate with the glue in an incubator for culture.
Case two:
1) Starting up, placing the automatic dispensing equipment on the operating platform 3, accessing the socket 9 on the operating cabinet 1, and precooling the operating platform 3;
2) When the temperature of the operation table 3 is reduced to 4 ℃, the glass clapboard is pulled down to seal the operation table 3, reagent consumables needing precooling are placed in the operation table 3, and ultraviolet disinfection is carried out for 15 minutes. Turning off the ultraviolet lamp 5;
3) The prepared cell matrix glue mixed solution is accessed into automatic equipment, and the automatic equipment is started and is subjected to glue dispensing;
4) And placing the culture plate with the glue in an incubator for culture.
To sum up, the utility model discloses following beneficial effect has:
1) By setting the circulating refrigeration, the table top of the operation table 3 and the surrounding environment thereof can reach 4 ℃, the low-temperature environment in the operation process of the matrigel can be effectively ensured, the icing phenomenon in the operation table 3 can be avoided, and the inconvenience of using an ice box for operation is overcome;
2) By arranging the sealing plate 10 and the glass window 7, the safety of personnel and the external environment can be ensured while the personnel can conveniently operate on the table top of the operating table 3, and the internal environment can be disinfected by arranging the ultraviolet lamp 5, so that the cleanness of the internal environment is ensured;
3) Through set up socket 9 on operation cabinet 1, can conveniently insert automatic small-size point glue equipment and accomplish automatic point glue operation on operation panel 3.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious changes and modifications can be made without departing from the scope of the invention.
Claims (10)
1. The utility model provides a preparation 3D cell culture glue drips low temperature superclean bench, includes operation cabinet (1), its characterized in that: one or more compressors (2) are installed to the bottom of operation cabinet (1), one or more compressor (2) with operation cabinet (1) electric connection, operation cabinet (1) is gone up and is located one or more the top of compressor (2) is provided with operation panel (3), the rear side of operation panel (3) is equipped with light (4), ultraviolet lamp (5) and cold wind board (6), light (4) with ultraviolet lamp (5) are installed on operation cabinet (1) and with operation cabinet (1) electric connection, the front side of operation panel (3) is equipped with glass window (7), cold wind board (6) fixed mounting be in operation cabinet (1) is gone up and has seted up a plurality of cold wind holes (8).
2. The cryogenic clean bench for producing 3D cell culture gel drops according to claim 1, characterized in that one or more sockets (9) are provided on the rear side of the bench (3).
3. The low-temperature ultra-clean operating platform for preparing 3D cell culture gel drops as claimed in claim 1, wherein sealing plates (10) are arranged on the left side and the right side of the operating platform (3), and the sealing plates (10) are fixedly arranged on the operating cabinet (1).
4. The low-temperature ultra-clean operating platform for preparing 3D cell culture gel drops as claimed in claim 1, wherein a control panel (11) is arranged below the operating platform (3), and the control panel (11) is fixedly installed on the operating cabinet (1).
5. The low-temperature ultra-clean console for preparing 3D cell culture gel drops according to claim 4, wherein a plurality of heat dissipation holes (12) are formed in one side of the control panel (11), and the heat dissipation holes (12) are formed in the console cabinet (1).
6. The cryogenic ultra-clean bench for preparing 3D cell culture gel drops according to claim 1, characterized in that a plurality of moving wheels (13) are mounted at the bottom of the operating cabinet (1).
7. The low-temperature ultra-clean operating platform for preparing 3D cell culture gel drops is characterized in that a closed chamber (14) is arranged above the operating platform (3), and sealing partition plates (15) are arranged on the left side and the right side of the closed chamber (14).
8. The low temperature clean bench for preparing 3D cell culture gel drops according to claim 1, characterized in that the bench (3) is made of stainless steel material.
9. The low temperature clean bench for preparing 3D cell culture gel drops according to claim 3, characterized in that the sealing plate (10) is made of glass material.
10. The low-temperature clean bench for preparing 3D cell culture gel drops according to claim 1, characterized in that the length, width and height of the operating cabinet (1) are 1200mm, 800mm and 1600mm respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222366042.XU CN218516771U (en) | 2022-09-06 | 2022-09-06 | Low-temperature ultra-clean operating platform for preparing 3D cell culture glue drops |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222366042.XU CN218516771U (en) | 2022-09-06 | 2022-09-06 | Low-temperature ultra-clean operating platform for preparing 3D cell culture glue drops |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218516771U true CN218516771U (en) | 2023-02-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222366042.XU Active CN218516771U (en) | 2022-09-06 | 2022-09-06 | Low-temperature ultra-clean operating platform for preparing 3D cell culture glue drops |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218516771U (en) |
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2022
- 2022-09-06 CN CN202222366042.XU patent/CN218516771U/en active Active
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