CN114348462A - Circulating tumor cell separation and enrichment kit - Google Patents
Circulating tumor cell separation and enrichment kit Download PDFInfo
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- CN114348462A CN114348462A CN202111575139.5A CN202111575139A CN114348462A CN 114348462 A CN114348462 A CN 114348462A CN 202111575139 A CN202111575139 A CN 202111575139A CN 114348462 A CN114348462 A CN 114348462A
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- 208000005443 Circulating Neoplastic Cells Diseases 0.000 title claims abstract description 29
- 238000000926 separation method Methods 0.000 title abstract description 12
- 238000004321 preservation Methods 0.000 claims abstract description 52
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 28
- 210000004027 cell Anatomy 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 238000009434 installation Methods 0.000 claims abstract description 8
- 210000004369 blood Anatomy 0.000 claims description 25
- 239000008280 blood Substances 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 4
- 238000005057 refrigeration Methods 0.000 abstract description 13
- 230000004087 circulation Effects 0.000 abstract description 10
- 239000003814 drug Substances 0.000 abstract description 10
- 210000004881 tumor cell Anatomy 0.000 abstract description 8
- 238000004378 air conditioning Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 239000003761 preservation solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241001391944 Commicarpus scandens Species 0.000 description 2
- 230000003028 elevating effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000002512 chemotherapy Methods 0.000 description 1
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- 230000000694 effects Effects 0.000 description 1
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- 238000012544 monitoring process Methods 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/38—Devices for discharging contents
- B65D25/52—Devices for discharging successive articles or portions of contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
- B65D81/26—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators
- B65D81/263—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants with provision for draining away, or absorbing, or removing by ventilation, fluids, e.g. exuded by contents; Applications of corrosion inhibitors or desiccators for ventilating the contents
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- Life Sciences & Earth Sciences (AREA)
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- Food Science & Technology (AREA)
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- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of cytobiology and discloses a circulating tumor cell separation and enrichment kit which comprises a kit main body and an operation table arranged in the kit main body, wherein a reagent storage mechanism is arranged in the kit main body and comprises an installation plate lapped on a bottom plate in the kit main body, and a lifting mechanism and a refrigerating mechanism are fixedly connected above the installation plate. This circulation tumor cells separation enrichment kit blows out the convection current wind through the convection current fan, at kit main part inner loop, carry out the convection current, guarantee the inside gas exchange of kit main part, make the inside reagent storage device of kit main part be in among a better environment, carry out work through refrigeration mechanism, refrigerate, make the inside ultra-low temperature that forms of reagent storage device, guarantee the cold-stored of medicament, the heat preservation box then can be refrigerated to cell preservation liquid pipe this moment, avoid the air conditioning to run off.
Description
Technical Field
The invention relates to the technical field of cell biology, in particular to a circulating tumor cell separation and enrichment kit.
Background
Circulating tumor cells are tumor cells that fall off from the primary and metastatic foci of solid tumors and enter the human peripheral blood circulation. Compared with other normal cells in peripheral blood, the circulating tumor cells are derived from tumor tissues, generally have the characteristics of large cell volume, small density, low rigidity, high membrane capacitance and the like, and also express epithelial marker proteins and specific proteins of certain source tissues.
The circulating tumor cell detection is used as a 'liquid biopsy' means for real-time tumor monitoring, so that the physiological and psychological pains of the tissue biopsy of a tumor patient are relieved, the clinician can be assisted to evaluate the overall life cycle and the progression-free life cycle of the patient, monitor the curative effect of clinical radiotherapy and chemotherapy, predict the tumor metastasis and recurrence, early warning and the like, and the method has important clinical significance for guiding individualized medical treatment and improving the survival state of the tumor patient.
The detection of circulating tumor cells has been challenging. In actual clinical work, blood testing is not immediate in many cases, and even requires long-distance transport, so that the accuracy of blood testing is related to the cell preservation solution used. The existing cell preservation solution also has the following problems: low-temperature storage is required, but the low-temperature storage puts higher requirements on the stability and the transportation condition of the sample; the circulating tumor cells can not be stored for a long time at normal temperature, so that the collected circulating tumor cells are very easy to break and degrade within a few hours, the form and epitope of the circulating tumor cells can not be effectively protected, the repeatability and reliability of analysis and detection of the circulating tumor cells can not be ensured, and the follow-up analysis and detection are not facilitated. And the number of circulating tumor cells in the peripheral blood of a patient is very small, so that the whole blood sample containing the circulating tumor cells is very unstable in the transportation process, and meanwhile, the whole blood sample storage container for storing the circulating tumor cells is too simple and is very inconvenient to take when the whole blood sample test tube is taken.
Therefore, in order to solve the problems, a kit for separating and enriching the circulating tumor cells is provided.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a circulating tumor cell separation and enrichment kit, which solves the problem that low-temperature storage is required, but the low-temperature storage puts higher requirements on the stability and the transportation condition of a sample; the circulating tumor cells can not be stored for a long time at normal temperature, so that the collected circulating tumor cells are very easy to break and degrade within a few hours, the form and epitope of the circulating tumor cells can not be effectively protected, the repeatability and reliability of analysis and detection of the circulating tumor cells can not be ensured, and the subsequent analysis and detection are not facilitated.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a circulation tumor cell separation enrichment kit, includes the kit main part and installs the inside operation panel in the kit main part, the mechanism is deposited to reagent has been placed to the inside of kit main part, the mechanism is deposited including the mounting panel of overlap joint on the inside bottom plate of kit main part to reagent, the top fixedly connected with elevating system and the refrigeration mechanism of mounting panel, elevating system's top is provided with first fixed plate, the top of first fixed plate is provided with the second fixed plate, the spout has been seted up to the surface of first fixed plate, the sliding block has been placed to the inside of spout, the top fixedly connected with mount table of second fixed plate, whole blood sample preservation pipe has been placed to the top of mount table, the top of second fixed plate is provided with the heat preservation box, the inside grafting of heat preservation box has cell preservation liquid pipe.
The lifting mechanism comprises a driving motor, a driving screw is movably connected to the inner side of the driving motor, one end of a rod body of the driving screw is connected to the driving motor, the other end of the rod body is movably connected with a cross rod, rollers are arranged at two ends of the cross rod, a sliding track is lapped on the outer side of each roller, one end of a shaft lever is fixedly connected to the outer side of each roller, and a lifting plate is fixedly connected to the other end of the rod body of the shaft lever; the refrigerating mechanism comprises a refrigerating pipe arranged in the refrigerating mechanism, and refrigerating sheets are arranged on the outer surface of the refrigerating pipe.
Further, an operation table is arranged inside the kit main body, the bottom end of the kit main body is fixedly connected with four groups of supporting seats, and the four groups of supporting seats are fixedly arranged at four corners of the kit main body and support the kit main body.
Further, the fixed surface of kit main part installs the fixture block, the outside movable mounting of kit main part has the pivot, one side swing joint that the kit main part was kept away from in the pivot has the lid, the kit main part with pass through between the lid the upset is connected in the pivot.
Furthermore, the outside fixedly connected with buckle of lid, the buckle with the fixture block phase-match can the joint be in the same place.
Furthermore, the outer surface of the kit main body is provided with convection fans which are oppositely arranged and are arranged on two groups of opposite side walls of the kit main body.
Furthermore, the axostylus axostyle is provided with two sets ofly, and two sets of the bottom of axostylus axostyle all is provided with the gyro wheel, the horizontal pole is connected between two sets of the gyro wheel.
Furthermore, the inside of heat preservation box is seted up evenly distributed and with the hole that cell preservation fluid pipe size accords with.
The technical scheme of the invention has the following beneficial effects:
1. this circulation tumor cell separation enrichment kit is provided with the mount table through the top of second fixed plate, and the whole blood sample save tube that the mount table top was placed and the heat preservation box that second fixed plate top was connected rise, promote suitable high position with whole blood sample save tube and heat preservation box, conveniently take.
2. This circulation tumor cell separation enrichment kit inserts in the inside of heat preservation box through cell preservation fluid pipe, can keep warm to the built-in liquid medicine of cell preservation fluid pipe, avoids the temperature to run off.
3. This circulation tumor cells separation enrichment kit works through refrigeration mechanism, and refrigeration pipe also works simultaneously with the refrigeration piece, refrigerates for reagent deposits the inside ultra-low temperature that forms of mechanism, guarantees the cold-stored of medicament, and the heat preservation box then can be refrigerated to cell preservation liquid pipe this moment, avoids the air conditioning to run off.
4. This circulation tumor cell separation enrichment kit, through the slip around the second fixed plate, whole blood sample storage tube and the heat preservation box placed on the second fixed plate vibrate in step then to make whole blood sample storage tube and the inside liquid medicine of cell preservation liquid vibrate the mixture, need the staff slowly to take when avoiding mixing and mix one by one, waste time and energy.
5. This circulation tumor cell separation enrichment kit blows out convection current wind through the convection current fan, at the inside circulation of kit main part, carries out the convection current, guarantees the inside gas exchange of kit main part for the inside reagent of kit main part is in a better environment to deposit the mechanism.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the connecting structure of the reagent storing mechanism in FIG. 1 according to the present invention;
FIG. 3 is a schematic view of the structure of FIG. 2 in a disassembled state according to the present invention;
FIG. 4 is a schematic view of the lower cross-sectional structure of FIG. 3 according to the present invention;
FIG. 5 is a schematic view of the connection structure of the lifting mechanism of the present invention;
FIG. 6 is an enlarged schematic view of the refrigeration mechanism of the present invention;
FIG. 7 is an enlarged view of the overall structure of the lifting mechanism of the present invention;
FIG. 8 is an enlarged view of the structure of area A in FIG. 7 according to the present invention.
In the figure: 1. a kit body; 2. an operation table; 3. a reagent storage mechanism; 4. a supporting seat; 5. a clamping block; 6. a rotating shaft; 7. a box cover; 8. buckling; 9. a convection fan;
31. mounting a plate; 35. a second fixing plate; 36. an installation table; 37. a whole blood sample storage tube; 38. a heat preservation box; 39. a cell preservation solution tube;
32. a lifting mechanism; 321. a drive motor; 322. a drive screw; 323. a cross bar; 324. a roller; 325. a sliding track; 326. a shaft lever; 327. a lifting plate;
33. a refrigeration mechanism; 331. a refrigeration pipe; 332. a refrigeration plate;
34. a first fixing plate; 341. a chute; 342. and a slider.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the present disclosure, terms such as "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "side", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only relational terms determined for convenience in describing structural relationships of the parts or elements of the present disclosure, and do not refer to any parts or elements of the present disclosure, and are not to be construed as limiting the present disclosure.
In the present disclosure, terms such as "fixedly connected", "connected", and the like are to be understood in a broad sense, and mean either a fixed connection or an integrally connected or detachable connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present disclosure can be determined on a case-by-case basis by persons skilled in the relevant art or technicians, and are not to be construed as limitations of the present disclosure.
Referring to fig. 2-4 and 6, a kit for separating and enriching circulating tumor cells includes a kit main body 1 and an operation table 2 installed inside the kit main body 1, a reagent storage mechanism 3 is placed inside the kit main body 1, the reagent storage mechanism 3 includes an installation plate 31 overlapping on a bottom plate inside the kit main body 1, a lifting mechanism 32 and a refrigerating mechanism 33 are fixedly connected above the installation plate 31, a first fixing plate 34 is arranged above the lifting mechanism 32, a second fixing plate 35 is arranged above the first fixing plate 34, a sliding chute 341 is formed on an outer surface of the first fixing plate 34, a sliding block 342 is placed inside the sliding chute 341, an installation table 36 is fixedly connected above the second fixing plate 35, a whole blood sample storage tube 37 is placed above the installation table 36, a heat preservation box 38 is arranged above the second fixing plate 35, and a cell preservation tube 39 is inserted inside the heat preservation box 38, the inside of the heat-insulating box 38 is provided with holes which are uniformly distributed and have the same size as the cell-storing liquid tube 39. The refrigeration mechanism 33 includes a refrigeration tube 331 provided inside thereof, and a refrigeration sheet 332 is provided on an outer surface of the refrigeration tube 331.
The cell preservation liquid tube 39 is inserted in the heat preservation box 38 in an inverted manner, so that the liquid medicine in the cell preservation liquid tube 39 can be preserved in heat, and the temperature loss is avoided. Meanwhile, when the refrigerating mechanism 33 works, the refrigerating tube 331 and the refrigerating piece 332 work simultaneously to refrigerate, so that the reagent storage mechanism 3 is internally at ultra-low temperature to ensure the cold storage of the reagent, and the heat preservation box 38 can refrigerate the cell preservation liquid tube 39 to avoid the loss of cold air.
When needs vibrate the mixture to the built-in liquid medicine of whole blood sample preservation pipe 37 and cell preservation liquid pipe 39, the user only needs the hand to hold second fixed plate 35, pulling around going on, second fixed plate 35 slides on the sliding block 342 that sets up on first fixed plate 34 then, sliding block 342 is synchronous slides inside spout 341, second fixed plate 35 is then the slip around realizing, whole blood sample preservation pipe 37 and heat preservation box 38 of placing on the second fixed plate 35 vibrate then in step, thereby make whole blood sample preservation pipe 37 and the inside liquid medicine of cell preservation liquid pipe 39 vibrate the mixture, need the staff to slowly take when avoiding mixing and mix gradually, waste time and energy.
Referring to fig. 5 and fig. 7-8, the lifting mechanism 32 includes a driving motor 321, a driving screw 322 is movably connected to an inner side of the driving motor 321, one end of a rod body of the driving screw 322 is connected to the driving motor 321, the other end of the rod body is movably connected to a cross bar 323, rollers 324 are respectively disposed at two ends of the cross bar 323, a sliding rail 325 is lapped on an outer side of each roller 324, two sets of shafts 326 are disposed, the rollers 324 are disposed at bottom ends of the two sets of shafts 326, the cross bar 323 is connected between the two sets of rollers 324, one end of the shaft 326 is fixedly connected to an outer side of each roller 324, and a lifting plate 327 is fixedly connected to the other end of the rod body of each shaft 326. When the reagent in the reagent kit body 1 needs to be extracted, the reagent kit body 1 is opened, the driving motor 321 in the lifting mechanism 32 is controlled to start working, the driving motor 321 immediately drives the driving screw 322 to start rotating, the driving screw 322 then drives the cross rod 323 to move, the two ends of the rod body of the cross rod 323 are connected with the rollers 324, the rollers 324 then slide on the sliding rails 325 along with the movement of the cross rod 323, the shaft rods 326 connected with the rollers 324 synchronously move along with the rolling of the rollers 324, then the lifting plate 327 is pushed outwards, the lifting plate 327 then drives the first fixing plate 34 connected above the lifting plate to ascend, the first fixing plate 34 immediately drives the second fixing plate 35 connected above the first fixing plate to ascend synchronously, the mounting table 36 is arranged above the second fixing plate 35, the whole blood sample storage tube 37 arranged above the mounting table 36 and the heat preservation box 38 connected above the second fixing plate 35 also ascend synchronously, the whole blood sample storage tube 37 and the incubator 38 are lifted to appropriate height positions, and the whole blood sample storage tube and the incubator are convenient to take.
Please refer to fig. 1, an operation table 2 is arranged inside a kit main body 1, a supporting seat 4 is fixedly connected to the bottom end of the kit main body 1, four groups of supporting seats 4 are arranged, the supporting seats are fixedly arranged at four corners of the kit main body 1 to support the kit main body 1, a clamping block 5 is fixedly arranged on the outer surface of the kit main body 1, a rotating shaft 6 is movably arranged at the outer side of the kit main body 1, a box cover 7 is movably connected to one side of the rotating shaft 6 away from the kit main body 1, the kit main body 1 and the box cover 7 are connected and turned over through the rotating shaft 6, a buckle 8 is fixedly connected to the outer side of the box cover 7, the buckle 8 is matched with the clamping block 5 and can be clamped together, a convection fan 9 is arranged on the outer surface of the kit main body 1, the convection fan 9 is arranged relatively, and is arranged on two groups of relative side walls of the kit main body 1.
For the convenience of understanding the above technical solutions of the present invention, the following detailed description is made of the working principle or the operation mode of the present invention in the practical process:
the working principle is as follows: when using, deposit mechanism 3 with reagent and put into the inside of kit main part 1, when not using, the staff can detain buckle 8 on fixture block 5, get up kit main part 1 closing cap, preserve or take, simultaneously, be provided with convection current fan 9 on the lateral wall of kit main part 1, convection current fan 9 can blow off convection current wind, at 1 internal circulation of kit main part, carry out the convection current, guarantee the inside gas exchange of kit main part 1, make the inside reagent of kit main part 1 deposit mechanism 3 and locate in a better environment.
When the reagent in the reagent kit body 1 needs to be extracted, the reagent kit body 1 is opened, the driving motor 321 in the lifting mechanism 32 is controlled to start working, the driving motor 321 immediately drives the driving screw 322 to start rotating, the driving screw 322 then drives the cross rod 323 to move, the two ends of the rod body of the cross rod 323 are connected with the rollers 324, the rollers 324 then slide on the sliding rails 325 along with the movement of the cross rod 323, the shaft rods 326 connected with the rollers 324 synchronously move along with the rolling of the rollers 324, then the lifting plate 327 is pushed outwards, the lifting plate 327 then drives the first fixing plate 34 connected above the lifting plate to ascend, the first fixing plate 34 immediately drives the second fixing plate 35 connected above the first fixing plate to ascend synchronously, the mounting table 36 is arranged above the second fixing plate 35, the whole blood sample storage tube 37 arranged above the mounting table 36 and the heat preservation box 38 connected above the second fixing plate 35 also ascend synchronously, the whole blood sample storage tube 37 and the heat preservation box 38 are lifted to proper height positions, so that the whole blood sample storage tube and the heat preservation box are convenient to take; meanwhile, the cell preservation liquid tube 39 is inserted in the heat preservation box 38 in an inverted manner, so that the liquid medicine in the cell preservation liquid tube 39 can be preserved, and the temperature loss is avoided. Meanwhile, when the refrigerating mechanism 33 works, the refrigerating tube 331 and the refrigerating piece 332 work simultaneously to refrigerate, so that the reagent storage mechanism 3 is internally at ultra-low temperature to ensure the cold storage of the reagent, and the heat preservation box 38 can refrigerate the cell preservation liquid tube 39 to avoid the loss of cold air.
When needs vibrate the mixture to the built-in liquid medicine of whole blood sample preservation pipe 37 and cell preservation liquid pipe 39, the user only needs the hand to hold second fixed plate 35, pulling around going on, second fixed plate 35 slides on the sliding block 342 that sets up on first fixed plate 34 then, sliding block 342 is synchronous slides inside spout 341, second fixed plate 35 is then the slip around realizing, whole blood sample preservation pipe 37 and heat preservation box 38 of placing on the second fixed plate 35 vibrate then in step, thereby make whole blood sample preservation pipe 37 and the inside liquid medicine of cell preservation liquid pipe 39 vibrate the mixture, need the staff to slowly take when avoiding mixing and mix gradually, waste time and energy.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (7)
1. A circulating tumor cell separating and enriching kit comprises a kit main body (1) and an operation table (2) arranged in the kit main body (1), wherein a reagent storage mechanism (3) is arranged in the kit main body (1);
the method is characterized in that: the reagent storage mechanism (3) comprises a mounting plate (31) lapped on the inner bottom plate of the reagent box main body (1), a lifting mechanism (32) and a refrigerating mechanism (33) are fixedly connected above the mounting plate (31), a first fixing plate (34) is arranged above the lifting mechanism (32), a second fixing plate (35) is arranged above the first fixing plate (34), a sliding groove (341) is formed in the outer surface of the first fixing plate (34), a sliding block (342) is placed in the sliding groove (341), an installation table (36) is fixedly connected above the second fixing plate (35), a whole blood sample storage tube (37) is arranged above the mounting table (36), a heat preservation box (38) is arranged above the second fixing plate (35), and a cell preservation liquid pipe (39) is inserted into the heat preservation box (38);
the lifting mechanism (32) comprises a driving motor (321), a driving screw (322), a cross rod (323), a roller (324), a sliding track (325), a shaft lever (326) and a lifting plate (327), wherein the driving screw (322) is movably connected to the inner side of the driving motor (321), one end of a rod body of the driving screw (322) is connected to the driving motor (321), the other end of the rod body is movably connected with the cross rod (323), the roller (324) is arranged at each of two ends of the cross rod (323), the sliding track (325) is lapped on the outer side of the roller (324), one end of the shaft lever (326) is fixedly connected to the outer side of the roller (324), and the lifting plate (327) is fixedly connected to the other end of the rod body of the shaft lever (326);
the refrigerating mechanism (33) comprises a refrigerating pipe (331) arranged inside the refrigerating mechanism, and a refrigerating piece (332) is arranged on the outer surface of the refrigerating pipe (331).
2. The kit for separating and enriching circulating tumor cells according to claim 1, wherein: the kit comprises a kit main body (1) and is characterized in that an operation table (2) is arranged inside the kit main body (1), the bottom end of the kit main body (1) is fixedly connected with four supporting seats (4), and the four supporting seats (4) are fixedly arranged at four corners of the kit main body (1) and support the kit main body (1).
3. The kit for separating and enriching circulating tumor cells according to claim 1, wherein: the utility model discloses a kit, including kit body (1), pivot (6), box cover (7), fixed surface installs fixture block (5) of kit body (1), the outside movable mounting of kit body (1) has pivot (6), keep away from pivot (6) one side swing joint of kit body (1) has lid (7), kit body (1) with pass through between lid (7) the upset is connected in pivot (6).
4. The kit for separating and enriching circulating tumor cells according to claim 3, wherein: the outside fixedly connected with buckle (8) of lid (7), buckle (8) with fixture block (5) phase-match can be in the same place the joint.
5. The kit for separating and enriching circulating tumor cells according to claim 1, wherein: the kit comprises a kit main body (1) and is characterized in that convection fans (9) are arranged on the outer surface of the kit main body (1), the convection fans (9) are oppositely arranged and are arranged on two groups of opposite side walls of the kit main body (1).
6. The kit for separating and enriching circulating tumor cells according to claim 1, wherein: the shaft rods (326) are provided with two groups, the bottom ends of the two groups of shaft rods (326) are provided with the rollers (324), and the cross rods (323) are connected between the two groups of rollers (324).
7. The kit for separating and enriching circulating tumor cells according to claim 1, wherein: holes which are uniformly distributed and have the same size with the cell preservation liquid pipe (39) are arranged in the heat preservation box (38).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111575139.5A CN114348462A (en) | 2021-12-21 | 2021-12-21 | Circulating tumor cell separation and enrichment kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111575139.5A CN114348462A (en) | 2021-12-21 | 2021-12-21 | Circulating tumor cell separation and enrichment kit |
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Application publication date: 20220415 |