CN218491722U - Selenium-enriched fermentation device based on selenium phosphate synthetase activity - Google Patents

Abstract

The utility model relates to a rich selenium yeast preparation technical field just discloses a rich selenium yeast device based on selenium phosphate synthetase activity, including preparing the case, the fixed baffle that is equipped with in inside of preparing the case, the fixed culture dish that is equipped with in upper end of baffle, the fixed grafting of terminal surface of preparation case has the raw materials to advance pipe, hydrogen peroxide to advance pipe and sodium selenite to advance the pipe, the raw materials advances pipe, hydrogen peroxide advances pipe and sodium selenite advances the pipe and is connected with vibration mechanism with the preparation case jointly, the fixed drain pipe that is equipped with in bottom of culture dish, and the inside of drain pipe is equipped with automatically controlled valve, the pipe end of drain pipe is connected with centrifugal separation mechanism. This application can improve the stability and the activity of selenium phosphate synthetase activity through adding hydrogen peroxide liquid and sodium selenite liquid in proper order, and the selenium-enriched yeast of output is of high quality.

Description

Selenium-enriched fermentation device based on selenium phosphate synthetase activity

Technical Field

The application relates to the technical field of selenium-enriched yeast preparation, in particular to a selenium-enriched yeast device based on selenium phosphate synthetase activity.

Background

Selenium-enriched yeast is added with selenium element in the process of culturing yeast, and the selenium is absorbed and utilized during the growth of the yeast, so that the selenium is organically combined with protein and polysaccharide in the yeast body and converted into biological selenium, and the selenium can be more efficiently and more safely absorbed and utilized by a human body.

In the course of implementing the present application, the inventors found that at least the following problems exist in the technology, in selenium-enriched yeast, selenoprotein is a main existing form of organic selenium, the content of selenoprotein is directly related to the capacity of yeast for converting inorganic selenium into organic selenium, and reflects the level of the organic selenium enrichment of yeast, in the biosynthesis of selenoprotein, selenophosphoric acid (SP) is an active selenium donor which is generated by Selenium Phosphate Synthase (SPs) in a catalytic manner, the SPs activity can directly reflect the capacity of the selenium-enriched yeast for enriching organic selenium, and in the preparation of the selenium-enriched yeast, how to stabilize and improve the activity of SPs in the selenium-enriched yeast is a problem which needs to be faced at present, therefore, a selenium-enriched yeast device based on the activity of the selenium phosphate synthase is provided.

SUMMERY OF THE UTILITY MODEL

The application aims to solve the problems that in the biosynthesis of selenoprotein in the prior art, selenophosphates (SP) are active selenium donors and are generated by Selenium Phosphate Synthetase (SPS) in a catalytic mode, the activity of the SPS can directly reflect the organic selenium enrichment capacity of selenium-enriched yeast, and how to stabilize and improve the activity of the SPS in the selenium-enriched yeast in the preparation of the selenium-enriched yeast is the problem which needs to be faced at present, and the selenium-enriched yeast device based on the activity of the selenophosphates synthetase is provided.

In order to achieve the purpose, the following technical scheme is adopted in the application:

the selenium-enriched fermentation device comprises a preparation box, wherein a partition plate is fixedly arranged in the preparation box, a culture dish is fixedly arranged at the upper end of the partition plate, a raw material inlet pipe, a hydrogen peroxide inlet pipe and a sodium selenite inlet pipe are fixedly inserted into the end face of the preparation box, the raw material inlet pipe, the hydrogen peroxide inlet pipe and the sodium selenite inlet pipe are jointly connected with a vibration mechanism, a liquid outlet pipe is fixedly arranged at the bottom of the culture dish, an electric control valve is arranged in the liquid outlet pipe, the pipe end of the liquid outlet pipe is connected with a centrifugal separation mechanism, an aseptic water inlet pipe is fixedly inserted into the pipe wall of the liquid outlet pipe and the side wall of the preparation box, a water inlet valve is arranged in the aseptic water inlet pipe, a discharge pipe is arranged at the discharge end of the centrifugal separation mechanism, a control valve is arranged in the discharge pipe, a circular hole matched with the discharge pipe is formed in the bottom of the preparation box, a temporary storage box is fixedly arranged in the bottom of the preparation box, and a backflow mechanism is jointly connected with the culture dish.

Preferably, the vibration mechanism advances the pipe including pegging graft in the raw materials jointly, hydrogen peroxide advances the pipe and sodium selenite advances the ring of tub outer tube wall, the fixed a plurality of archs that are equipped with of inner wall of ring, the top of ring is equipped with the disc, a set of bar hole has been seted up to the terminal surface of disc, and the inside in each bar hole all fixedly is equipped with the slide bar, each the equal sliding connection of pole wall of slide bar has the oscillating beam, each all fixed spring that is equipped with jointly between the pole wall in oscillating beam and the pore wall in homonymy bar hole, the upper end of disc is fixed and is equipped with the actuating lever, the fixed driving motor that is equipped with in upper end of preparation case, and driving motor's output and actuating lever are connected.

Preferably, the centrifugal separation mechanism is connected with a fixed gear in a rotating manner through a pipe wall arranged on the liquid outlet pipe, a separation sleeve is fixedly arranged at the lower end of the fixed gear, a chassis is fixedly arranged at the bottom of the separation sleeve, the discharge pipe is located on the end face of the chassis and is fixedly inserted and connected, a transmission rod is rotatably arranged on the end face of the partition plate, a movable gear meshed with the fixed gear is fixedly inserted and connected to the rod wall of the transmission rod, belt pulleys are fixedly sleeved on the rod walls of the transmission rod and the driving rod, and the two belt pulleys are connected together through a belt in a transmission manner.

Preferably, the backflow mechanism comprises a pump body fixedly arranged on the side wall of the temporary storage box, the suction end of the pump body is communicated with the temporary storage box, and the output end of the pump body and the side wall of the culture dish are fixedly inserted with a backflow pipe.

Preferably, a drain pipe is fixedly inserted at the lower end of the side wall of the preparation box.

Preferably, the number of the strip-shaped holes and the oscillating rods is 2.

Compared with the prior art, the application provides a rich selenium device based on selenium phosphate synthetase activity, possesses following beneficial effect:

1. this rich selenium yeast device based on selenium phosphate synthetase activity, through the preparation case that is equipped with, the baffle, the culture dish, the raw materials advances the pipe, the pipe is advanced to hydrogen peroxide, sodium selenite advances the pipe, the drain pipe, aseptic water advances the pipe, the discharging pipe, the temporary storage case, vibration mechanism, the mutually supporting of centrifugal separation mechanism and reflux mechanism, through the culture in-process at selenium-enriched yeast, add appropriate amount hydrogen peroxide liquid, and the oscillation carries out, can obtain the activity to selenium phosphate synthetase in the selenium-enriched yeast and carry out preliminary promotion, improve the stability and the activity of selenium phosphate synthetase activity.

2. The selenium-enriched yeast device based on the selenium phosphate synthetase activity is provided with centrifugal separation, sterile water is added, and after backflow, a proper amount of sodium selenite is added, so that the activity of selenium phosphate synthetase in selenium-enriched yeast can be further improved, and the quality of the produced selenium-enriched yeast is good.

The part that does not relate to in the device all is the same with prior art or can adopt prior art to realize, and this application can improve the stability and the activity of selenium phosphate synthetase activity through adding hydrogen peroxide liquid and sodium selenite liquid in proper order, and the quality of the selenium-enriched yeast of output is good.

Drawings

FIG. 1 is a schematic diagram of a selenium enrichment fermentation apparatus based on selenium phosphate synthase activity as set forth herein;

FIG. 2 is an enlarged view of the portion A in FIG. 1;

fig. 3 is a schematic top view of the ring of fig. 1.

In the figure: 1. preparing a box; 2. a partition plate; 3. a culture dish; 4. feeding the raw materials into a pipe; 5. a hydrogen peroxide inlet pipe; 6. sodium selenite is fed into the tube; 7. a liquid outlet pipe; 8. a sterile water inlet pipe; 9. a discharge pipe; 10. a temporary storage box; 12. a circular ring; 13. a protrusion; 14. a disc; 15. a slide bar; 16. an oscillating rod; 17. a spring; 18. a drive rod; 19. a drive motor; 20. a stationary gear; 21. separating the sleeve; 22. a chassis; 23. a transmission rod; 24. a belt; 25. a moving gear; 26. a pump body; 27. a return pipe; 28. and a liquid discharge pipe.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments.

Example 1:

referring to fig. 1-3, a selenium-enriched fermentation device based on selenium phosphate synthetase activity, which comprises a preparation box 1, a partition plate 2 is fixedly arranged inside the preparation box 1, a culture dish 3 is fixedly arranged at the upper end of the partition plate 2, a raw material inlet pipe 4, a hydrogen peroxide inlet pipe 5 and a sodium selenite inlet pipe 6 are fixedly inserted into the end face of the preparation box 1, the raw material inlet pipe 4, the hydrogen peroxide inlet pipe 5 and the sodium selenite inlet pipe 6 are jointly connected with the preparation box 1, a liquid outlet pipe 7 is fixedly arranged at the bottom of the culture dish 3, an electric control valve is arranged inside the liquid outlet pipe 7, a centrifugal separation mechanism is connected to the pipe end of the liquid outlet pipe 7, a sterile water inlet pipe 8 is fixedly inserted into the pipe wall of the liquid outlet pipe 7 and the side wall of the preparation box 1, a water inlet valve is arranged inside the sterile water inlet pipe 8, a discharge pipe 9 is arranged at the discharge end of the centrifugal separation mechanism, a control valve is arranged inside the discharge pipe 9, a circular hole matched with the discharge pipe 9 is formed in the bottom of the preparation box 1, a storage box 10 is fixedly arranged at the bottom of the preparation box 1, and a reflux mechanism are jointly connected with the culture dish 3.

The vibration mechanism comprises a circular ring 12 which is jointly inserted into the outer wall of a raw material inlet pipe 4, a hydrogen peroxide inlet pipe 5 and a sodium selenite inlet pipe 6, a plurality of bulges 13 are fixedly arranged on the inner wall of the circular ring 12, a disc 14 is arranged above the circular ring 12, a group of strip-shaped holes are formed in the end face of the disc 14, a slide rod 15 is fixedly arranged inside each strip-shaped hole, a vibration rod 16 is connected to the rod wall of each slide rod 15 in a sliding manner, a spring 17 is fixedly arranged between the rod wall of each vibration rod 16 and the hole wall of the strip-shaped hole on the same side, a drive rod 18 is fixedly arranged at the upper end of the disc 14, a drive motor 19 is fixedly arranged at the upper end of the preparation box 1, the output end of the drive motor 19 is connected with the drive rod 18, the drive rod 19 works to drive the disc 14 to rotate by matching with the drive rod 18, so that the plurality of vibration rods 16 can be driven to rotate, and further under the extrusion of the bulges 13 and the resilience of the spring 17, the vibration rod 16 can oscillate, and culture solution can be oscillated and mixed.

The backflow mechanism comprises a pump body 26 fixedly arranged on the side wall of the temporary storage box 10, the suction end of the pump body 26 is communicated with the temporary storage box 10, the output end of the pump body 26 and the side wall of the culture dish 3 are jointly and fixedly connected with a backflow pipe 27 in an inserted mode, and the pump body 26 works and can be matched with the backflow pipe 27 to suck mixed liquid back to the culture dish 3.

The number of a set of bar holes and oscillating bars 16 is 2 settings, and through this kind of setting, the oscillation effect can be ensured.

Example 2:

centrifugal separation mechanism sets up including rotating to rotate in the pipe wall of drain pipe 7 and rotates and is connected with quiet gear 20, the fixed separation cover 21 that is equipped with of lower extreme of quiet gear 20, the fixed chassis 22 that is equipped with in bottom of separation cover 21, and the fixed grafting setting of terminal surface that discharging pipe 9 is located chassis 22, the terminal surface rotation of baffle 2 is equipped with transfer line 23, the pole wall of transfer line 23 is fixed to be pegged graft and is had the moving gear 25 with quiet gear 20 engaged with, the band pulley has all been cup jointed to the pole wall of transfer line 23 and actuating lever 18, and two band pulleys are connected through 24 transmissions of belt jointly, cooperate band pulley and belt 24 effects through actuating lever 18, can make transfer line 23 rotate, thereby can cooperate moving gear 25 and quiet gear 20 to drive separation cover 21 and rotate, in order to carry out centrifugal separation work, need not to set up a plurality of driving pieces.

A liquid discharge pipe 28 is fixedly inserted into the lower end of the side wall of the preparation tank 1, and can discharge liquid generated during centrifugation.

In this application, during the use, advance pipe 4 through the raw materials and add appropriate amount of selenium-enriched yeast raw materials and culture solution earlier, after a period of cultivation, advance pipe 5 through hydrogen peroxide, can add appropriate amount of hydrogen peroxide solution in to the culture solution, after the oscillation, let in the separation cover 21 inside with mixed liquid through drain pipe 7, at this moment through the centrifugal separation of separation cover 21, obtain bacterial deposit, it lets in appropriate amount sterile water to advance pipe 8 through sterile water, and shift into temporary storage box 10 with sterile water and bacterial mixed liquid through discharging pipe 9, pump body 26 will be mixed liquid and take out, cooperation back flow 27, let in mixed liquid again in culture dish 3, it adds appropriate amount of sodium selenite liquid to advance pipe 6 through sodium selenite at last, through the centrifugal separation after the oscillation cultivation and once more, can obtain the selenium-enriched yeast bacterial after promoting.

The above description is only for the preferred embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art should be considered to be within the scope of the present application, and all equivalent substitutions and changes according to the technical solutions and the inventive concepts of the present application should be covered by the scope of the present application.

Claims (6)

1. The utility model provides a rich selenium yeast device based on selenium phosphate synthetase activity, is including preparing case (1), its characterized in that, the fixed baffle (2) that is equipped with in inside of preparing case (1), the fixed culture dish (3) that is equipped with in upper end of baffle (2), the fixed grafting of terminal surface of preparing case (1) has raw materials to advance pipe (4), hydrogen peroxide to advance pipe (5) and sodium selenite to advance pipe (6), raw materials advance pipe (4), hydrogen peroxide advances pipe (5) and sodium selenite advances pipe (6) and preparation case (1) and is connected with vibration mechanism jointly, the fixed drain pipe (7) that is equipped with in bottom of culture dish (3), and the inside of drain pipe (7) is equipped with automatically controlled valve, the pipe end of drain pipe (7) is connected with centrifugal separation mechanism, the pipe wall of drain pipe (7) and the lateral wall of preparing case (1) are fixed to be pegged graft jointly has aseptic water to advance pipe (8), and the inside that aseptic water advances pipe (8) is equipped with, centrifugal separation mechanism's discharge end is equipped with discharging pipe (9), and is equipped with the inside control valve (9) of preparing case (1) and preparation case (10) is equipped with temporary storage case (10) and temporary storage case (10) are connected with the backflow mechanism.

2. The selenium-enriched fermentation device based on the selenium phosphate synthetase activity is characterized in that the vibration mechanism comprises a circular ring (12) which is jointly inserted into the outer wall of a raw material inlet pipe (4), a hydrogen peroxide inlet pipe (5) and a sodium selenite inlet pipe (6), a plurality of bulges (13) are fixedly arranged on the inner wall of the circular ring (12), a circular disc (14) is arranged above the circular ring (12), a group of strip-shaped holes are formed in the end face of the circular disc (14), sliding rods (15) are fixedly arranged inside the strip-shaped holes, the rod wall of each sliding rod (15) is connected with an oscillating rod (16) in a sliding mode, a spring (17) is fixedly arranged between the rod wall of each oscillating rod (16) and the hole wall of the strip-shaped hole on the same side in a common mode, a driving rod (18) is fixedly arranged at the upper end of the circular disc (14), a driving motor (19) is fixedly arranged at the upper end of the preparation box (1), and the output end of the driving motor (19) is connected with the driving rod (18).

3. The selenium-enriched fermentation device based on the activity of selenium phosphate synthase according to claim 1, wherein the centrifugal separation mechanism comprises a static gear (20) rotatably connected to the wall of the liquid outlet pipe (7), a separation sleeve (21) is fixedly arranged at the lower end of the static gear (20), a chassis (22) is fixedly arranged at the bottom of the separation sleeve (21), the discharge pipe (9) is fixedly inserted and arranged on the end face of the chassis (22), a transmission rod (23) is rotatably arranged on the end face of the partition plate (2), a movable gear (25) meshed with the static gear (20) is fixedly inserted and arranged on the rod wall of the transmission rod (23), belt wheels are fixedly sleeved on the rod walls of the transmission rod (23) and the driving rod (18), and the two belt wheels are in transmission connection through a belt (24).

4. The selenium-enriched yeast device based on the selenium phosphate synthase activity according to claim 1, wherein the backflow mechanism comprises a pump body (26) fixedly arranged on the side wall of the temporary storage tank (10), the suction end of the pump body (26) is communicated with the temporary storage tank (10), and the output end of the pump body (26) and the side wall of the culture dish (3) are fixedly inserted with a backflow pipe (27).

5. The selenium-enriched fermentation device based on the activity of the selenium phosphate synthase according to claim 1, characterized in that a drain pipe (28) is fixedly inserted at the lower end of the side wall of the preparation box (1).

6. The selenium enrichment fermentation device based on the selenium phosphate synthase activity according to claim 2, wherein the number of the strip-shaped holes and the oscillating bars (16) is 2.

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