CN218483459U

CN218483459U - Hairy root growth culture apparatus

Info

Publication number: CN218483459U
Application number: CN202222510239.6U
Authority: CN
Inventors: 周晗; 邹衡芳; 赖云; 陈宁; 范航; 来昌冲
Original assignee: Guangzhou Yuanxiang Medical Biotechnology Co ltd
Current assignee: Guangzhou Yuanxiang Medical Biotechnology Co ltd
Priority date: 2022-09-22
Filing date: 2022-09-22
Publication date: 2023-02-17
Anticipated expiration: 2032-09-22

Abstract

The utility model discloses a bioreactor device for hairy root culture, which comprises a transparent tank body provided with a discharge port, a cover body arranged on the tank body and provided with a feed port, an air inlet and an air outlet, and an air breather arranged in the tank body, wherein the air breather comprises an air pipe with one end communicated with the air inlet and an annular air vent ring communicated with the other end of the air pipe, the side wall of the air vent ring is provided with a plurality of air vents, and the air vent ring is arranged in an area close to the bottom of the tank body; the utility model discloses a set up annular ring of ventilating to set up a plurality of air vents of a plurality of evenly distributed on the ring inside lateral wall of ventilating on ventilating the ring, reach the purpose to the even air feed of culture medium and hairy root, and the air vent that distributes and set up has increased the internal dissolved oxygen volume of jar, and then improve hairy root and get growth efficiency, gas outlet and jar internal connection, can discharge the waste gas that produces after tail gas processing apparatus handles, the setting of carrying the ear is more convenient in sterilization, cleaning and disinfecting.

Description

Hairy root growth culture apparatus

Technical Field

The utility model relates to a biological cultivation technical field especially relates to a hairy root growth culture apparatus.

Background

Hairy roots are also called hairy roots, hairy roots. Is a pathological state generated after agrobacterium rhizogenes infects plants. Its advantages are high productivity, high biologic synthesis power and high growth speed. Agrobacterium rhizogenes can infect not only whole plants or an organ, tissue (including callus), single cells or even protoplasts, and can produce a large amount of by-product, hairy roots, at or near the site of infection.

Agrobacterium rhizogenes is a gram-negative Agrobacterium tumefaciens of the genus Bacillus of the family Rhizobiaceae that infects most dicotyledonous and few monocotyledonous plants, even gymnosperms, to induce the plant to produce hairy roots. At present, the method is widely applied to the fields of gene engineering, plant secondary metabolism production, plant variety improvement, plant cultivation and the like, and in the aspect of medicinal plants, hairy roots generated by transforming plants by agrobacterium rhizogenes m plasmids establish an in vitro culture system to realize the industrial production of secondary metabolites so as to solve the problem of shortage of traditional Chinese medicine resources. Compared with conventional cell culture and tissue culture, the hairy root culture system has the advantages of rapid growth, no need of phytohormone, strong and stable capability of synthesizing secondary metabolites, capability of releasing partial metabolites like culture solution and the like, and by changing culture conditions, hairy roots can even synthesize active substances which are several times higher than that of the original plant and active ingredients which are not contained in the original plant.

At present, the existing hairy root culture device is often conveyed by a vent pipe in the process of introducing air or oxygen, but the vent pipe only carries out centralized conveying on a certain part during gas conveying, so that the amount of oxygen which can not be received by the hairy root in the culture device is uneven, or the dissolved oxygen in a tank body is not high, and the problem of poor culture effect is caused.

The microbial pollution which is another problem of hairy root culture is an important factor for limiting industrialization, no culture device can well control the microbial pollution at present, and the microbial pollution is often controlled by adding a large amount of antibiotics, so that the antibiotic residue in the product is serious, and the quality and the application of the product are greatly influenced.

In order to solve the problems, the utility model provides a hairy root growth culture device, which solves the problems of poor culture effect and easy microbial contamination of the prior hairy root culture device.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a hairy root growth culture device, which can improve the culture effect of hairy roots and effectively prevent the microbial contamination.

In order to achieve the above object, the utility model provides a following scheme:

the utility model provides a bioreactor device that hairy root was cultivateed, is in including the transparent jar body of seting up the discharge gate, setting the lid and the setting that set up feed inlet, air inlet and gas outlet on the jar body are in the internal breather device of jar, breather device include one end with the breather pipe of air inlet intercommunication and with the cyclic annular ring of ventilating of the other one end intercommunication of breather pipe, a plurality of air vents have been seted up on the lateral wall of ring of ventilating, the ring setting of ventilating is in the region that is close to jar body bottom.

Preferably, the plurality of vent holes are uniformly arranged on the inner ring side wall of the vent ring.

Preferably, the air purifier further comprises a one-way valve and a first air filter which are arranged on the air inlet in sequence from bottom to top.

Preferably, the edge region of the cover is provided with a pair of lifting lugs.

Preferably, still include the support, the support includes the landing leg, sets up lower connecting ring on the landing leg, set up perpendicularly and be in prevent guardrail bar and setting on the lower connecting ring are in prevent last connecting ring on the guardrail bar, the jar body sets up accept on the board.

Preferably, a sealing gasket is arranged at the joint of the tank body and the cover body.

Preferably, the air outlet is provided with a check valve, a condenser, a pressure gauge and a second air filter in sequence.

Preferably, still include tail gas processing apparatus, tail gas processing apparatus is including preventing down suction bottle and tail gas absorption bottle, prevent down suction bottle with the gas outlet is through first pipeline intercommunication, prevent down suction bottle with the tail gas absorption bottle passes through the second pipeline intercommunication.

The utility model discloses for prior art gain following technological effect:

1. the utility model discloses a set up annular ring of ventilating to set up a plurality of air vents of a plurality of evenly distributed on the ring inside ring lateral wall of ventilating on the ring of ventilating, reach the purpose to the even air feed of culture medium and hairy root, and the air vent that distributes the setting has increased the internal dissolved oxygen volume of jar, and then improves hairy root and gets growth efficiency.

2. The utility model also comprises a first air filter and a one-way valve which are arranged on the air inlet; the air filter is arranged to ensure that the air enters the tank body at the purity, harmful gas is filtered, and further hair roots are ensured to grow healthily, the one-way valve can control the air to enter at any time, and meanwhile, the air is prevented from escaping from the air outlet.

3. The utility model provides a device is provided with check valve, condenser, manometer, second air cleaner and is connected with tail gas processing apparatus on the gas outlet, and check valve and second air cleaner can prevent the gaseous pollutants backward flow, effectively prevents microbial contamination, and manometer detectable tank internal pressure avoids leading to the equipment explosion because of pressure is too big, and tail gas processing apparatus can handle tail gas, prevents the polluted air.

4. The utility model also comprises a bracket, the bracket comprises a landing leg, a lower connecting ring arranged on the landing leg, a protective railing vertically arranged on the lower connecting ring and an upper connecting ring arranged on the protective railing, and the tank body is arranged on the bearing plate; guarantee the stability of the jar body, the setting of rail guard has avoided the jar body to receive the collision damage simultaneously.

Drawings

In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive labor.

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the present invention;

wherein, 1, a bracket; 2. a vent hole; 3. a venting ring; 4. a tank body; 5. a guard rail; 6. a cover body; 7. lifting the ears; 8. an air outlet; 9. a pressure gauge; 10. a feed inlet; 11. an air inlet; 12. a breather pipe; 13. a bolt; 14. a one-way valve; 15. a discharge port; 16. an oxygen-dissolved electrode inlet; 17. a Ph electrode inlet; 18. a temperature probe inlet; 19. a sampling port; 20. a condensing unit; 21. a suck-back prevention bottle; 22. tail gas absorption bottle.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model aims at providing a hairy root growth culture device, which achieves the purposes of improving the culture effect of hairy roots and effectively preventing microbial pollution.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

Referring to fig. 1 to 2, a hairy root growth and culture device comprises a transparent tank 4 with a discharge hole 15 at the bottom, a cover 6 arranged on the tank 4 and provided with a feed inlet 10, an air inlet 11 and an air outlet 8, and an aeration device arranged in the tank 4, wherein the aeration device comprises an aeration pipe 12 with one end communicated with the air inlet 11 and an annular aeration ring 3 communicated with the other end of the aeration pipe 12, the side wall of the aeration ring is provided with a plurality of aeration holes 2, and the aeration ring 3 is arranged in a region close to the bottom of the tank 4; the utility model discloses a set up annular ring 3 of ventilating to set up a plurality of air vents 2 of a plurality of evenly distributed on the ring lateral wall of ventilating 3 inner rings of ring on ventilating 3, reach the purpose to the even air feed of culture medium and hairy root, and the air vent 2 that distributes the setting has increased the dissolved oxygen volume in the jar body 4, and then improves hairy root and gets growth efficiency.

Referring to fig. 1, a plurality of air vents 2 are uniformly arranged on the inner ring side wall of an air vent ring 3 to ensure that air uniformly and stably contacts with a culture medium and hairy roots.

Referring to fig. 1, the air purifier further comprises a check valve 14 and a first air filter which are arranged on the air inlet 11 in sequence from bottom to top; through setting up the purity of first air cleaner assurance air into jar body 4, filter harmful gas, and then guarantee that hairy root gets healthy growth, the setting of check valve 14 can carry out the entering of control air at any time, avoids the air to escape from gas outlet 8 simultaneously.

Referring to fig. 1 or 2, the edge region of the cover 6 is provided with a pair of lifting lugs 7; the tank body 4 is convenient to take.

Referring to fig. 1, the multifunctional support further comprises a support 1, the support 1 comprises support legs, lower connecting rings arranged on the support legs, protective railings 5 vertically arranged on the lower connecting rings, and upper connecting rings arranged on the protective railings 5, and the tank body 4 is arranged on the bearing plate; guarantee the stability of the jar body 4, the setting of preventing guardrail bar 5 has simultaneously avoided the jar body 4 to receive the collision damage.

Further, a sealing gasket is arranged at the joint of the tank body 4 and the cover body 6.

Referring to fig. 1, a one-way valve 14, a condenser, a pressure gauge 9 and a second air filter are sequentially arranged at the air outlet 8.

Referring to fig. 2, the exhaust gas treatment device is further included, and the exhaust gas treatment device includes a back suction prevention bottle 21 and an exhaust gas absorption bottle 22, and the back suction prevention bottle 21 is communicated with the gas outlet 8 through a first pipeline, and the back suction prevention bottle 21 is communicated with the exhaust gas absorption bottle through a second pipeline, and the first pipeline is provided with a condensing device 20.

Referring to fig. 2, the cover 6 is further provided with a dissolved oxygen electrode inlet 16, a Ph electrode inlet 17, a temperature probe inlet 18, and a sampling port 19.

The specific culture method of the hairy root is as follows:

adding about 3L of culture medium into a 5L glass tank 4, tightly sealing the glass tank 4 and the cover 6 through a sealing washer and a bolt 13, and finally putting the whole body in a sterilizing pot for sterilization;

when the culture medium in the glass tank body 4 is cooled to room temperature, the sterile hairy roots are pumped in from the feed inlet 10 in a peristaltic pump mode;

and starting the air compressor, wherein compressed air firstly passes through the first air filter, then passes through the one-way valve 14, and finally is stably and uniformly introduced into the tank body 4 through the vent holes in the vent ring 3 at the bottom of the tank body 4.

Air and waste gas are discharged through an air outlet 8 on the cover body 6, and the air outlet 8 is externally connected with a second air filter and a tail gas absorption bottle 22.

The adaptability that carries out according to actual demand all is in the protection scope of the utility model.

It should be noted that, as is apparent to those skilled in the art, the present invention is not limited to the details of the above-described exemplary embodiments, but can be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a hairy root growth culture apparatus, its characterized in that, including the transparent jar body of seting up the discharge gate, set up and be in the lid and the setting that set up feed inlet, air inlet and gas outlet are in on the jar body the internal breather device of jar, breather device include one end with the breather pipe of air inlet intercommunication and with the cyclic annular ring of ventilating of the other one end intercommunication of breather pipe, a plurality of air vents have been seted up on the lateral wall of ventilating the ring, ventilate the ring setting and be close to the region of jar body bottom.

2. The apparatus according to claim 1, wherein the plurality of ventilation holes are uniformly arranged on the inner side wall of the ventilation ring.

3. The apparatus according to claim 1, further comprising a check valve and a first air filter disposed on the air inlet in the order from bottom to top.

4. A hairy root growth and culture device according to claim 1, wherein the edge region of the cover is provided with a pair of lifting lugs.

5. The apparatus according to claim 1, further comprising a support frame, the support frame comprising a support leg, a lower connecting ring disposed on the support leg, a guard rail vertically disposed on the lower connecting ring, and an upper connecting ring disposed on the guard rail, wherein the tank body is disposed on the receiving plate.

6. The apparatus for growing and culturing hairy root according to claim 1, wherein the joint of the pot and the cover is provided with a sealing gasket.

7. The apparatus for growing and culturing hairy roots according to claim 1, wherein the air outlet is sequentially provided with a check valve, a condenser, a pressure gauge and a second air filter.

8. The hairy root growth culture device of claim 7, further comprising a tail gas treatment device, wherein the tail gas treatment device comprises a back suction prevention bottle and a tail gas absorption bottle, the back suction prevention bottle is communicated with the gas outlet through a first pipeline, and the back suction prevention bottle is communicated with the tail gas absorption bottle through a second pipeline.