CN218478730U - Separating device - Google Patents

Abstract

The embodiment of the utility model discloses separator relates to peripheral blood mononuclear cell separation technical field. The separation device comprises a sampling unit, a sample adding unit, a connecting piece, a standing bag and a transfer bag; the sampling unit, the sample adding unit and the static bag are all connected with the connecting piece, and the connecting piece is used for communicating the static bag with the sampling unit and the sample adding unit; the standing bag is connected with the transfer bag. The separation device can rapidly realize rapid separation of peripheral blood mononuclear cells.

Description

Separating device

Technical Field

The utility model relates to a peripheral blood mononuclear cell separation technical field especially relates to a separator.

Background

Peripheral Blood Mononuclear Cells (PBMCs) mainly refer to lymphocytes and monocytes, are the most commonly used cells in immunological experiments, and have strong in vitro culture and amplification potential.

At present, the treatment of the mononuclear cell is researched and popularized in a large range at home and abroad, the disease can induce the self-antiviral immune response through in vitro culture, proliferation and activation and is infused back into the body, and once the human antiviral immunity is activated, antiviral substances are continuously generated to kill viruses. After being activated, most of T cells with the function of killing tumor cells become memory cells to be stored in lymphatic tissues, thereby providing long-term protection for thoroughly eliminating the tumor cells and preventing and treating metastasis and relapse.

With the increase of age, the activity of the mononuclear cells in the peripheral blood in vivo is reduced, so that the mononuclear cells with higher activity are frozen in a young period, and the method has important significance for future immunotherapy. However, there is currently no device that can rapidly isolate peripheral blood mononuclear cells.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a technical problem that will solve provides a device that can quickly separating peripheral blood mononuclear cell.

In order to solve the above problems, an embodiment of the present invention provides a separation device, which includes a sampling unit, a sample adding unit, a connecting member, a standing bag, and a transfer bag; the sampling unit, the sample adding unit and the static bag are all connected with the connecting piece, and the connecting piece is used for communicating the static bag with the sampling unit and the sample adding unit; the standing bag is connected with the transfer bag.

The technical scheme is that the sampling device comprises a sampling unit, a sample adding unit and a standing bag, wherein the sampling unit is connected with the sampling unit, the sample adding unit is connected with the standing bag, and the standing bag is connected with the sampling unit.

The sampling unit comprises a puncture outfit and a first liquid conveying pipe, wherein two ends of the first liquid conveying pipe are respectively connected with the puncture outfit and the connector of the three-way pipe.

The further technical proposal is that the tail end of the puncture outfit is a tip with a triangular shape.

The technical scheme is that the liquid conveying device further comprises a first switch, and the first switch is arranged on the first liquid conveying pipe.

The technical scheme is that the sample adding unit comprises a second liquid conveying pipe and a rubber plug, the rubber plug is arranged at one end of the second liquid conveying pipe in a sealing mode, and the other end of the second liquid conveying pipe is connected with an interface of the three-way pipe.

The separation device further comprises a third liquid conveying pipe, and two ends of the third liquid conveying pipe are respectively connected with the standing bag and the connector of the three-way pipe.

The separation device further comprises a fourth liquid conveying pipe and a second switch, wherein the fourth liquid conveying pipe is respectively connected with the standing bag and the transfer bag; the second switch is arranged on the fourth liquid conveying pipe.

The still standing bag comprises a first inner cavity, the first inner cavity comprises a first side and a second side which are opposite, the inner diameter of the first inner cavity is gradually reduced from the first side to the second side, and the fourth liquid conveying pipe is connected to the second side.

The further technical scheme is that the transfer bag further comprises a closed pipeline, and the closed pipeline is connected with the transfer bag. The closed pipeline can be cut by a tool to communicate with the external container if necessary, and the sample in the transfer bag is transferred into the external container.

Compared with the prior art, the embodiment of the utility model provides a technical effect that can reach includes:

the utility model provides a separator can add the peripheral blood sample in the blood sampling bag to the bag of stewing through the connecting piece through sampling device. Further, a preparation required for separating peripheral blood mononuclear cells, such as hydroxyethyl starch, can be added to the standing bag through the connection member by the sample addition unit. The tubing between the resting bag and the connector is then sealed (e.g., by heat sealing) and the resting bag is hung upside down so that the transfer bag is on the top side of the resting bag. When the supernatant and the sediment in the standing bag are layered, the supernatant is transferred into the transfer bag by slowly extruding the standing bag, so that the peripheral blood mononuclear cells in the supernatant are quickly separated.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without any creative effort.

Fig. 1 is a schematic structural diagram of a separation device according to an embodiment of the present invention.

Reference numerals

The sampling unit 10, the sample adding unit 20, the connecting member 30, the standing bag 40, the transfer bag 50, the first switch 60, the third liquid delivery pipe 70, the fourth liquid delivery pipe 80, the second switch 90, the closed pipeline 100, the three-way pipe 31, the interface 32, the puncture outfit 11, the first liquid delivery pipe 12, the tip 111, the second liquid delivery pipe 21, the rubber plug 22, the first inner cavity 41, the first side 411, the second side 412 and the second inner cavity 51.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, wherein like component numbers represent like components. It is obvious that the embodiments to be described below are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It will be understood that the terms "comprises" and/or "comprising," when used in this specification and the appended claims, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

It is also to be understood that the terminology used in the description of the embodiments of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the embodiments of the invention. As used in the description of the embodiments of the present invention and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

Referring to fig. 1, the embodiment of the present invention provides a separation device, which includes a sampling unit 10, a sample adding unit 20, a connecting member 30, a resting bag 40, and a transfer bag 50. The components are specifically described as follows:

the sampling unit 10, the sample adding unit 20 and the static bag 40 are all connected with the connecting piece 30, and the connecting piece 30 is used for communicating the static bag 40 with the sampling unit 10 and the sample adding unit 20. The resting pouch 40 is connected to the transfer pouch 50.

A peripheral blood sample in the blood collection bag can be added to the static bag 40 through the connector 30 by a sampling device. Further, a preparation, such as hydroxyethyl starch, required for separating peripheral blood mononuclear cells can be added to the resting bag 40 through the connection member 30 by the sample addition unit 20. The tubing between the resting pouch 40 and the connector 30 (i.e., the third liquid delivery tube 70) is then cut and sealed (e.g., by heat sealing) and the resting pouch 40 is left standing upside down so that the transfer pouch 50 is on the upper side of the resting pouch 40. When the supernatant and the sediment in the standing bag 40 are layered, the supernatant is transferred into the transfer bag 50 by slowly squeezing the standing bag 40, so that the peripheral blood mononuclear cells in the supernatant are quickly separated.

Further, the connecting member 30 is a three-way pipe 31, the three-way pipe 31 includes three connectors 32, and the three connectors 32 of the three-way pipe 31 are respectively connected to the sampling unit 10, the sample adding unit 20, and the resting bag 40. The stationary bag 40 can be communicated with the sampling unit 10 and the sample adding unit 20 through a three-way pipe 31.

Further, the sampling unit 10 includes a puncture instrument 11 and a first liquid conveying pipe 12, and both ends of the first liquid conveying pipe 12 are respectively connected to the puncture instrument 11 and a connector 32 of the three-way pipe 31. The peripheral blood sample is usually stored in a blood collection bag, and the peripheral blood sample can be sampled by inserting the puncture instrument 11 into the open end of the screw cap of the blood collection bag and tightening the puncture instrument. The first liquid delivery pipe 12 may be embodied as a silicone hose. The length of the first liquid delivery tube 12 may be specifically 20cm.

Further, the end of the puncture device 11 is a tip 111 with a triangular shape, similar to a needle shape, for being inserted into the open end of the cap of the blood collection bag.

Further, a first switch 60 is included, and the first switch 60 is disposed on the first liquid conveying pipe 12. The first switch 60 is used to turn on or off the first liquid delivery tube 12. In this embodiment, the first switch 60 is specifically a water trap, and those skilled in the art may also adopt other switch devices, which is not limited to the present invention.

Further, the sample adding unit 20 includes a second liquid conveying pipe 21 and a rubber plug 22, the rubber plug 22 is hermetically disposed at one end of the second liquid conveying pipe 21, and the other end of the second liquid conveying pipe 21 is connected to the interface 32 of the three-way pipe 31. The preparation, such as hydroxyethyl starch, required for the isolation of peripheral blood mononuclear cells can be delivered into the resting bag 40 by syringe through the plug 22. The plug 22 effects sealing on the one hand and enables delivery of the formulation on the other hand. The second liquid delivery pipe 21 may be embodied as a silicone hose.

Further, the separation device further comprises a third liquid conveying pipe 70, and two ends of the third liquid conveying pipe 70 are respectively connected with the stationary bag 40 and the interface 32 of the three-way pipe 31. The third liquid conveying pipe 70 is used for communicating the standing bag 40 and the three-way pipe 31. The third liquid delivery pipe 70 may be embodied as a silicone hose. The length of the third liquid delivery pipe 70 may be specifically 20cm.

In one embodiment, the seal of the static pouch 40 may be accomplished by sealing the third liquid delivery tube 70, for example, in one embodiment, the seal may be a heat seal. Further, the sealed end of the third liquid delivery pipe 70 near the three-way pipe 31 can be cut off, so that the separation device becomes simpler to complete the subsequent peripheral blood separation operation.

Further, the separation device further comprises a fourth liquid delivery pipe 80 and a second switch 90, wherein the fourth liquid delivery pipe 80 is respectively connected with the resting bag 40 and the transfer bag 50; the second switch 90 is disposed on the fourth liquid delivery pipe 80. The fourth fluid transfer tube 80 is used to communicate the resting bag 40 with the transfer bag 50. The second switch 90 is used to effect on/off control of the fourth liquid transport pipe 80. In this embodiment, the second switch 90 is specifically a pulley speed adjusting clamp, and the pulley speed adjusting clamp can also control the liquid flow. Other switching devices may be used by those skilled in the art, and the present invention is not limited thereto. The fourth liquid delivery pipe 80 may be embodied as a silicone hose. The length of the fourth liquid delivery tube 80 may be specifically 20cm.

Further, the stationary bag 40 comprises a first inner cavity 41, the first inner cavity 41 comprises a first side 411 and a second side 412 which are opposite, the inner diameter of the first inner cavity 41 is gradually reduced from the first side 411 to the second side 412, and the fourth liquid delivery pipe 80 is connected to the second side 412. It can be seen that first lumen 41 is approximately triangular in shape and the sharp corner of the triangle (i.e., second side 412) connects to the fourth fluid delivery tube 80. Thereby, the sediment can be concentrated on the first side 411 of the first lumen 41, facilitating separation of the supernatant. The first lumen 41 may specifically have a capacity of 200ml.

Further, the transfer bag 50 includes a second lumen 51, the second lumen 51 being approximately square in shape. The volume of the second lumen 51 may be specifically 100ml.

Further, a closed pipeline 100 is further included, and the closed pipeline 100 is connected with the transfer bag 50. The closed conduit 100 may be cut with a tool to communicate with the external container as necessary to transfer the sample in the transfer bag 50 to the external container. For example, when the separated supernatant in the transfer bag 50 is to be transferred, the closed line 100 is sterilized with 75% alcohol in advance, and then the line is cut with sterile scissors, and the supernatant is transferred to the centrifuge tube through the cut closed line 100. The length of the closed pipe 100 may be 10cm.

In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "secured" are to be construed broadly and can, for example, be connected or detachably connected or integrated; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by one skilled in the art.

It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, while the invention has been described with respect to certain embodiments, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.

The above description is for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of various equivalent modifications or replacements within the technical scope of the present invention, and these modifications or replacements should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. A separation device is characterized by comprising a sampling unit, a sample adding unit, a connecting piece, a standing bag and a transfer bag; the sampling unit, the sample adding unit and the static bag are all connected with the connecting piece, and the connecting piece is used for communicating the static bag with the sampling unit and the sample adding unit; the standing bag is connected with the transfer bag.

2. The separation device of claim 1, wherein the connector is a tee comprising three ports, and the three ports of the tee are respectively connected with the sampling unit, the sample adding unit and the standing bag.

3. The separation device of claim 2, wherein the sampling unit comprises a puncture outfit and a first liquid conveying pipe, and both ends of the first liquid conveying pipe are respectively connected with the puncture outfit and the interface of the tee.

4. The separation device of claim 3, wherein the piercer terminates in a tip that is triangular in shape.

5. The separator arrangement of claim 3, further comprising a first switch disposed on the first liquid conveying conduit.

6. The separation device of claim 2, wherein the sample adding unit comprises a second liquid conveying pipe and a rubber plug, the rubber plug is arranged at one end of the second liquid conveying pipe in a sealing manner, and the other end of the second liquid conveying pipe is connected with the interface of the three-way pipe.

7. The separator of claim 2, further comprising a third liquid delivery pipe, wherein two ends of the third liquid delivery pipe are respectively connected with the standing bag and the connector of the tee.

8. The separation device of claim 1, further comprising a fourth liquid delivery tube and a second switch, the fourth liquid delivery tube being connected to the resting bag and the transfer bag, respectively; the second switch is arranged on the fourth liquid conveying pipe.

9. The separation device of claim 8, wherein the resting bag comprises a first lumen comprising opposing first and second sides, the first lumen having an inner diameter that gradually decreases from the first side to the second side, the fourth liquid delivery tube being connected to the second side.

10. The separation device of claim 1, further comprising a closed conduit connected to the transfer bag.

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