CN218404255U - Water-proof accurate temperature control cell rapid resuscitation instrument - Google Patents

Abstract

The utility model relates to a quick resuscitator of accurate accuse temperature cell of water proof formula, its characterized in that: the device comprises a water tank (1), a freezing pipe jacket (2) and a heating element (3), wherein the water tank (1) and the freezing pipe jacket (2) form a closed cavity (11), a heat-conducting medium (4) is injected into the cavity (11), and the heating element (3) is arranged in the heat-conducting medium (4). The utility model discloses small and difficult pollution, the recovery time is short and with low costs.

Description

Water-proof accurate temperature control cell rapid resuscitation instrument

Technical Field

The invention relates to a cell resuscitator, in particular to a water-proof type accurate temperature control cell rapid resuscitator.

Background

Modern biology, particularly cell biology, is the direction. Cell culture is an essential and commonly used experimental method and means. In the cell culture process, cells frozen in a low-temperature environment such as an ultra-low-temperature refrigerator or liquid nitrogen are often taken out, recovered and cultured. The traditional cell recovery method is that the cells are taken out and put into water bath with 37 ℃ for unfreezing and recovery. The traditional water bath recovery method has the advantages that: 1, the method is quick, can recover multitubular cells in a large amount at one time, and generally lasts for about 3 minutes; 2, the water bath kettle, an experimental device used in the economical and recovery process, has low price and only needs about thousand yuan; 3, the cell survival rate is high, the temperature in the whole process is less than or equal to 37 ℃, and the death rate of the cells in the recovery process can be reduced as much as possible.

However, the water bath kettle used in the traditional water bath recovery method also has some inevitable disadvantages: 1, the volume is large, the volume of the traditional water bath kettle water tank is large and is generally more than 3L, and the volume is more than 10L by adding other heating and control accessories; 2, the water in the water bath kettle is easy to pollute, and the water in the water bath kettle is easy to breed microorganisms such as bacteria, fungi and the like, and is a fatal potential risk for cell culture.

It is particularly important to design a cell recovery device which can exert the advantages of water bath recovery and avoid the defects of the water bath recovery device.

Disclosure of Invention

The utility model aims at providing a water proof type accurate temperature control cell rapid resuscitator which is small in size and not easy to pollute.

The utility model discloses accurate accuse temperature cell of water-proof formula is recovery appearance fast, including basin, freezing deposit pipe clamp cover and heating element, the basin with freeze and deposit pipe clamp cover and form an inclosed cavity, inject heat-conducting medium in the cavity, heating element sets up in heat-conducting medium. The closed water tank can prevent the heat-conducting medium from contacting with the outside. The injected heat-conducting medium is sterilized in advance, so that sterility is guaranteed. The freezing tube jacket is completely attached to the outer wall of the freezing tube, and heat is conducted in a contact mode, so that the recovery time of cells can be effectively shortened. The freezing pipe jacket can be provided in plurality.

Preferably, a temperature control system is further included, the temperature control system controlling heating of the heating element. The temperature control system is designed and researched by itself, an algorithm is designed by itself on the basis of an experiment, rapid heating or accurate temperature control is carried out according to the temperature condition of the sealing liquid and requirements, the recovery time can be shortened, and meanwhile the high survival rate of cells can be guaranteed.

More preferably, the heat transfer medium is water. The specific heat of water is large, and the constant temperature effect is good.

Preferably, the heat transfer medium is subjected to a sterilization treatment.

More preferably, the thickness of the outer wall of the freezing storage pipe jacket is 0.2 mm-0.5 mm. The inner cavity of the freezing storage pipe jacket is attached to the outer wall of the freezing storage pipe, the outer wall of the freezing storage pipe jacket is as thin as possible and ranges from 0.2mm to 0.5mm, and the conduction efficiency can be improved. The thickness and the inner cavity shape of the freezing storage pipe jacket can be designed according to different freezing storage pipes.

Preferably, the freezing tube jacket is made of a metal material. The metal material has good heat conduction performance, can ensure that the outer wall of the freezing pipe jacket is as thin as possible, can provide enough strength, and ensures the service life.

Preferably, the freezing storage pipe jacket adopts one of aluminum alloy, copper or silver.

Preferably, the heating element is a resistance wire or a ceramic sheet.

More preferably, the heating element is water repellent treated. The heating element can be directly put into water, and the heating efficiency is improved.

Compared with the prior art, the utility model discloses small and difficult pollution, the recovery time is short and with low costs.

Drawings

The present invention will be described and explained in detail with reference to the preferred embodiments with reference to the accompanying drawings.

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 shows an embodiment of the present invention.

1 is a water tank, 2 is a freezing pipe jacket, 3 is a heating element, 4 is a heat-conducting medium, 5 is a temperature control system, 11 is a chamber, 21 is an outer wall, and 51 is a switch.

Detailed Description

As shown in FIG. 1 and FIG. 2, the utility model discloses accurate accuse temperature cell of water-proof formula is recovery appearance fast, including basin 1, cryopreserved pipe clamp cover 2 and heating element 3, basin 1 and cryopreserved pipe clamp cover 2 form an inclosed cavity 11, inject heat-conducting medium 4 in the cavity 11, and heating element 3's one end sets up in heat-conducting medium 4. The other end of the heating element 3 is connected with a temperature control system 5.

The closed water tank 1 can prevent the heat-conducting medium 4 from contacting with the outside. And the injected heat-conducting medium 4 is sterilized in advance to ensure sterility. The freezing tube jacket 2 is completely attached to the outer wall 21 of the freezing tube, and heat is conducted in a contact manner, so that the recovery time of cells can be effectively shortened. The freezing pipe jacket 2 can be provided in plurality. The heat transfer medium 4 is preferably sterilized water.

The utility model discloses still include temperature control system 5, temperature control system 5 control heating element 3's heating. The temperature control system 5 is designed and researched automatically, an algorithm is designed automatically on the basis of an experiment, rapid heating or accurate temperature control is carried out according to the temperature condition of the heat-conducting medium 4 and requirements, so that the recovery time can be shortened, and meanwhile, the high survival rate of cells can be guaranteed.

The thickness of the outer wall 21 of the freezing tube jacket 2 is 0.2 mm-0.5 mm. The inner cavity of the freezing storage pipe jacket 2 is attached to the outer wall 21 of the freezing storage pipe, the outer wall 21 of the freezing storage pipe jacket 2 is as thin as possible and is 0.2 mm-0.5 mm, and the conduction efficiency can be improved. The thickness and the inner cavity shape of the freezing tube jacket 2 can be designed according to different freezing tubes. The freezing storage pipe jacket 2 adopts one of aluminum alloy, copper or silver.

The heating element 3 adopts a resistance wire or a ceramic plate. The heating element 3 is subjected to a water repellent treatment. The heating element 3 can be directly put into water, and the heating efficiency is improved.

The utility model discloses a water proof accurate accuse temperature cell rapid resuscitation appearance mainly includes airtight basin 1, freezes deposits pipe jacket 2, heating element 3, temperature control system 5. The closed water tank 1 can prevent water from contacting with the outside, and injected water is sterilized in advance to ensure sterility; the inner shape of the freezing tube is designed to be completely fit with the outer wall 21 of the freezing tube, and the recovery time of cells can be shortened by conducting heat in a contact manner; the heating element 3 is also customized by the company, so that the heating speed is ensured, and the accuracy is also considered; temperature control system 5 self-designed research and development, and the algorithm is designed by oneself on the basis of the experiment, according to the temperature condition of sealing liquid, carries out rapid heating or accurate temperature control as required, can guarantee when the time of resuscitating shortens, can guarantee again that the survival rate of cell is high.

The utility model discloses an innovation point lies in:

1. the design of the closed water tank 1 can ensure the temperature stability and accuracy of the resuscitation process, and can prevent water from being exposed, thereby avoiding the pollution of water breeding bacteria to cell culture.

2. The inner cavity of the freezing storage pipe jacket 2 is designed by being attached to the outer wall 21 of the freezing storage pipe, and the pipe wall is as thin as possible (0.2-0.5 mm), so that the conduction efficiency can be improved; the freezing storage pipe can be designed and customized according to different freezing storage pipes; metal materials (such as aluminum alloy, copper and silver) with good heat conduction performance are adopted, so that the pipe wall is thin, sufficient strength can be provided, and the service life is ensured;

3. the customized heating element 3 adopts a resistance wire or a ceramic plate to carry out strict waterproof treatment, and can be directly put into water, so that the heating efficiency is improved;

4. based on experimental test results, the customized temperature control plate can control the heating power of the heating element 3 according to the self-designed algorithm under different temperature conditions, so that an optimal survival rate capable of ensuring cell resuscitation is obtained, and the resuscitation time is short.

Claims (9)

1. The utility model provides a quick resuscitator of accurate accuse temperature cell of water proof formula which characterized in that: the device comprises a water tank (1), a freezing pipe jacket (2) and a heating element (3), wherein the water tank (1) and the freezing pipe jacket (2) form a closed cavity (11), a heat-conducting medium (4) is injected into the cavity (11), and the heating element (3) is arranged in the heat-conducting medium (4).

2. The water-stop type precise temperature control cell rapid resuscitator according to claim 1, wherein: the heating device further comprises a temperature control system (5), wherein the temperature control system (5) controls the heating of the heating element (3).

3. The water-resisting precise temperature control cell rapid resuscitator of claim 2, wherein: the heat-conducting medium (4) is water.

4. The water-stop type precise temperature control cell rapid resuscitator according to claim 3, wherein: the heat-conducting medium (4) is sterilized.

5. The water-stop type precise temperature control cell rapid resuscitator according to claim 4, wherein: the thickness of the outer wall (21) of the freezing storage pipe jacket (2) is 0.2-0.5 mm.

6. The instrument according to any one of claims 4 or 5, wherein: the freezing pipe jacket (2) is made of metal materials.

7. The water-stop type precise temperature control cell rapid resuscitator according to claim 6, wherein: the freezing storage pipe jacket (2) is made of one of aluminum alloy, copper or silver.

8. The instrument according to any one of claims 1 or 7, wherein: the heating element (3) adopts a resistance wire or a ceramic chip.

9. The water-stop type precise temperature control cell rapid resuscitator according to claim 8, wherein: the heating element (3) is subjected to a water repellent treatment.

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