CN218339828U - Viscous micropump based on algae cells - Google Patents
Viscous micropump based on algae cells Download PDFInfo
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- CN218339828U CN218339828U CN202122967603.7U CN202122967603U CN218339828U CN 218339828 U CN218339828 U CN 218339828U CN 202122967603 U CN202122967603 U CN 202122967603U CN 218339828 U CN218339828 U CN 218339828U
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Abstract
The utility model provides an alga cell-based viscidity micropump, including fluid drive chip, L type keysets, three-dimensional nanometer moving platform, the microscope, CCD, high-accuracy Z displacement platform, the projecting apparatus, focus objective, signal generator and computer, the vertical board left surface fixed connection of L type keysets is on three-dimensional nanometer moving platform, the horizontal board right side middle part of L type keysets is equipped with the ladder rectangular channel, fluid drive chip matches to inlay and establishes in the upper portion groove of ladder rectangular channel, the microscope sets up directly over fluid drive chip, the lens cone of microscope and high-accuracy Z displacement platform fixed connection, the projecting apparatus sets up directly under fluid drive chip, focus objective sets up in the camera lens upper portion of projecting apparatus, focus objective's light path and the coincidence of microscope, signal generator is connected with fluid drive chip electricity, the computer is respectively with CCD and projecting apparatus signal connection. The utility model discloses utilize rotatory algae cell to accomplish the drive to the fluid, do not need the external world additionally to provide the energy.
Description
Technical Field
The utility model relates to a biological fluid drive technical field, specific theory relates to a viscidity micropump based on algae cell.
Background
Micro total analysis systems, also known as lab-on-a-chip, have since been proposed for widespread use in chemistry, life sciences and microelectronics. The micro total analysis system can be used as a micro filter for filtering and collecting micro particles, can also be used as a micro mixer for mixing samples and analytes in the fields of chemistry, biology and the like, and even can be used as a micro reactor for polymerase chain reaction. In order to realize the above-mentioned applications, in a micro total analysis system, it is often necessary to integrate some functional components, including: the flow sensor, the micro valve, the micropump, among these several functional components, the micropump is the most critical element, and the micropump can be used for driving the controllable transmission of fluid in the micro total analysis system.
Conventional micro-electromechanical system (MEMS) based micro-pumps can be divided into two broad categories, mechanical micro-pumps and non-mechanical micro-pumps. The viscous micropump belongs to an improved mechanical micropump, realizes the driving of fluid by utilizing a rotating cylinder at an asymmetric position of a microchannel, has the high efficiency of the traditional mechanical micropump, and also has the stability of a non-mechanical micropump. In viscous micropumps, however, it is a very challenging task to fabricate micro-actuators capable of 360 degree rotation.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a viscidity micropump based on algae cell, the utility model discloses utilize rotatory algae cell to accomplish the drive to the fluid, do not need the external extra energy that provides.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a viscous micropump based on algae cells comprises a fluid driving chip, an L-shaped adapter plate, a three-dimensional nano moving platform, a microscope, a CCD, a high-precision Z-direction displacement platform, a projector, a focusing objective lens, a signal generator and a computer, wherein the fluid driving chip is horizontally arranged, a fluid inlet and a fluid outlet are arranged on the upper surface of the fluid driving chip at intervals from left to right, a micro pipeline positioned between the fluid inlet and the fluid outlet is arranged in the fluid driving chip along the left-right direction, the fluid inlet is connected with the left end of the micro pipeline, the fluid outlet is connected with the right end of the micro pipeline, the L-shaped adapter plate comprises a horizontal plate and a vertical plate, the left side of the horizontal plate of the L-shaped adapter plate is fixedly connected with the lower side of the vertical plate in an integrated forming manner, the left side of the vertical plate of the L-shaped adapter plate is fixedly connected to the three-dimensional nano moving platform, the horizontal plate right side middle part of L type keysets is equipped with the upside, downside and right side are all uncovered and big-end-up's ladder rectangular channel, the fluid drive chip is the same with the size in the upper portion groove of ladder rectangular channel, the fluid drive chip matching inlays to be established in the upper portion groove of ladder rectangular channel, the microscope sets up directly over the fluid drive chip, CCD fixed mounting is on microscopical upper portion, microscopical lens cone and high-accuracy Z are to displacement platform fixed connection, the projector sets up directly under the fluid drive chip, the camera lens setting of projector is at the top of projector and upwards shine, focus objective sets up on the camera lens upper portion of projector, focus objective's light path and microscope's light path coincidence, signal generator is connected with the fluid drive chip electricity, the computer is respectively with CCD and projector signal connection.
The fluid driving chip comprises an upper ITO glass layer and a lower ITO glass layer, a microstructure layer is arranged between the upper ITO glass layer and the lower ITO glass layer, the length directions of the upper ITO glass layer and the lower ITO glass layer are both arranged along the left-right direction, the upper ITO glass layer and the lower ITO glass layer are both in a rectangular sheet structure with the length of 25mm and the width of 20mm, the thickness of the upper ITO glass layer and the thickness of the lower ITO glass layer are both 1mm, two first through holes with the diameter of 0.5mm and through up and down are arranged on the upper ITO glass layer at intervals left and right, the first through hole on the left side is a fluid inlet, the first through hole on the right side is a fluid outlet, the microstructure layer is in a square sheet structure with the side length of 20mm, the thickness of the microstructure layer is 0.02-0.1mm, a rectangular long hole with the length of 10mm and through up and down is arranged in the middle of the left and right directions of the microstructure layer, two second through holes with the diameter of 0.5mm and through up and down are arranged on the microstructure layer at intervals left and right, the rectangular long hole is positioned between the two second through holes, the left end of the rectangular long hole is communicated with the left second through hole, the right end of the rectangular long hole is communicated with the right second through hole, the lower surface of the upper ITO glass layer and the upper surface of the lower ITO glass layer are conductive surfaces, the signal generator is respectively electrically connected with the two conductive surfaces through a wire, a hydrogenated amorphous silicon layer is coated on the conductive surface of the lower ITO glass layer in a spinning mode, the area of the hydrogenated amorphous silicon layer is a square area with the side length of 20mm, the thickness of the hydrogenated amorphous silicon layer is 1 mu m, the right side edge of the hydrogenated amorphous silicon layer is flush with the right side edge of the upper surface of the lower ITO glass layer, the conductive surface of the upper ITO glass layer is bonded with the upper surface of the micro-structure layer, the left side edge of the lower surface of the upper ITO glass layer is flush with the left side edge of the upper surface of the micro-structure layer, the upper surface of the hydrogenated amorphous layer is bonded with the lower surface of the micro-structure layer, and the micro-structure layer is aligned with the hydrogenated amorphous layer up and down, the two first through holes are respectively aligned with the two second through holes up and down correspondingly, and the rectangular long hole is surrounded into a micro pipeline with the length of 10mm, the width of 0.1mm and the height of 0.02-0.1mm after the upper ITO glass layer, the micro-structural layer and the lower ITO glass layer are bonded.
The L-shaped adapter plate is an aluminum plate, the width of the L-shaped adapter plate is 50mm, the thickness of the L-shaped adapter plate is 2mm, the height of a vertical plate of the L-shaped adapter plate is 50mm, the length of a horizontal plate of the L-shaped adapter plate is 200mm, the size of an upper groove of the stepped rectangular groove is 25mm long, 20mm wide and 0.5mm thick, and the size of a lower groove of the stepped rectangular groove is 24mm long, 18mm wide and 1.5mm thick.
An operation method of a viscous micro-pump based on algae cells specifically comprises the following steps:
firstly, assembling and connecting a viscous micropump;
(II) slowly injecting the treated algae cell solution into the micro-pipeline in the fluid driving chip from the fluid inlet;
drawing a plurality of light spot images on a computer by using flash drawing software, wherein each light spot image is projected upwards through a projector, converged through a focusing objective lens and then projected upwards through a gap of a stepped rectangular groove to the lower surface of the fluid driving chip;
adjusting the three-dimensional nano moving platform and the high-precision Z-direction displacement platform to enable the focal plane of the focusing objective lens and the focal plane of the microscope to be focused at the micro-pipeline inside the fluid driving chip;
fifthly, adjusting the size of the light spot drawn in the step three, adjusting the size of the light spot finally projected on the fluid driving chip to be 10-15 mu m, and then starting a signal generator, wherein the signal generator generates a sinusoidal signal with the frequency of 60KHz and the peak value of 6V and acts on the fluid driving chip through a lead;
capturing autorotation algae cells in the micro-pipeline by using the projected light spots and moving the algae cells to arrange and arrange;
and (seventh), removing the redundant algae cells from the micro-pipe, and turning off the signal generator and the projector, so that the algae cells rotating in the micro-pipe drive the fluid to flow in the micro-pipe from the fluid inlet to the fluid outlet.
The step (II) is specifically as follows: collecting 1ml of algae cell solution, centrifuging at 3000rpm for 6min in a centrifuge, removing supernatant, adding deionized water, and adjusting the concentration of the centrifuged algae cell solution to 1 × 10 5 cells/ml, and then injecting the treated algal cell solution from the fluid inlet into the microchannel within the fluid driven chip.
The reason why the size of the light spot finally projected onto the fluid driving chip is adjusted to 10-15 μm in the step (five) is as follows: the shape of the algae cells is elliptical or oval, the size of the algae cells is about 10 mu m, and the size of the light spot is adjusted to be equivalent to that of the algae cells, so that the capture operation of single algae cells is more convenient to realize;
and (V) the sinusoidal signal generated by the signal generator acts on the conductive surface of the upper ITO glass layer and the conductive surface of the lower ITO glass layer through the conducting wires respectively.
The step (six) is specifically as follows: feeding back images in the fluid driving chip observed by a microscope to a computer through a CCD, moving each light spot drawn by flash drawing software on the computer by combining the fed-back images, further moving the light spots projected onto the fluid driving chip, respectively capturing autorotation algae cells in the micro-pipeline by each light spot, arranging each clockwise rotation algae cell at a position close to the wall of the front side pipeline in the micro-pipeline at a left-right interval, and arranging each anticlockwise rotation algae cell at a position close to the wall of the rear side pipeline in the micro-pipeline at a left-right interval.
The rotating mechanism of the algae cells in the step (VI) is as follows: the front end of the body of the algae cell is provided with two flagella, the two flagella of the algae cell belong to a linear motor and can only generate a certain degree of bending swing, the bending swing of the two flagella of the algae cell is generated by relative sliding caused by the conformational change of dynein between adjacent microtubules, when both the two flagella are in a free state, the algae cell shows a random swimming motion form, and when only a single flagella is in the free swing state, the algae cell shows a counterclockwise rotation or clockwise rotation motion form.
The mechanism of capturing and moving algae cells by the light spots in the step (six) is as follows: when no light spot irradiates the ITO glass layer, the conductivity of the hydrogenated amorphous silicon layer is very low, the hydrogenated amorphous silicon layer can be regarded as an insulating layer, the voltage loaded on the fluid driving chip is basically applied to the hydrogenated amorphous silicon layer, when the light spot irradiates the hydrogenated amorphous silicon layer, the conductivity of the hydrogenated amorphous silicon layer in a spot area is increased rapidly due to electron hole pairs generated by the light spot, the hydrogenated amorphous silicon layer in the spot area is equivalent to a conductor, most of the voltage falls on the microstructure layer, a non-uniform electric field is generated in the microstructure layer in the spot area and the spot-free area, algae cells are polarized in the non-uniform electric field area and further subjected to light-induced dielectrophoresis force, and the expression of the light-induced dielectrophoresis force borne by the algae cells is as follows:
in the formula
Is the dielectric constant of the solution medium,
is the diameter of the algal cells and is,
is a C-M factor
The real part of (a) is,
a gradient that is the square of the root mean square value of the electric field;
when the signal generator generates a sinusoidal signal with the frequency of 60KHz and the peak-to-peak value of 6V and acts on the conductive surface of the upper ITO glass layer and the conductive surface of the lower ITO glass layer of the fluid driving chip through the conducting wire, the algae cells are subjected to light-induced dielectrophoresis force of the facula-free area pointing to the facula area, and the light-induced dielectrophoresis force is larger than the own motility force of the algae cells, so that the facula can capture and move the algae cells.
The step (VII) is specifically as follows: locking the arranged algae cells by using each light spot, and injecting deionized water into the micro-pipeline from the fluid inlet at a certain flow rate, wherein the flow rate of the deionized water meets the following conditions: the fluid drag force on the algae cells locked by the light spots needs to be smaller than the light-induced dielectrophoresis force on the algae cells, so that the rest algae cells which are not locked by the light spots are washed away from the micro-pipe by the deionized water, and then the signal generator and the projector are closed, so that the rotating algae cells in the micro-pipe drive the fluid to flow from the fluid inlet to the fluid outlet.
The mechanism that the algae cells rotating in the micro-pipe drive the fluid to flow from the fluid inlet to the fluid outlet in the micro-pipe in the step (seven) is as follows: because the clearance of the algae cell of rotation and the pipeline wall of rear side in the microchannel is different with its clearance with the pipeline wall of front side in the microchannel, then can cause the algae cell to rotate the in-process, the fluid viscous resistance that the algae cell both sides received is different, will produce a net flow to the fluid when the algae cell is rotatory like this, drives the fluid and can follow the left end of microchannel and flow right end: the row of the algae cells rotating anticlockwise close to the wall of the rear side pipeline in the micro-pipeline can drive the fluid to flow from the fluid inlet to the fluid outlet in the micro-pipeline, the row of the algae cells rotating clockwise close to the wall of the front side pipeline in the micro-pipeline can also drive the fluid to flow from the fluid inlet to the fluid outlet in the micro-pipeline, and the driving effects generated by the algae cells rotating in opposite directions on the two sides are consistent.
Compared with the prior art, the utility model have substantive characteristics and progress, specifically speaking, the utility model discloses regard rotatory algae cell as the functional unit in the traditional micropump, both can avoid making accurate micro actuator's difficulty like this, also solved the problem that the energy was supplied with simultaneously, utilize the energy of biological cell self to accomplish the drive to the fluid, do not need the external extra energy that provides, utilize a plurality of algae cells collaborative work, can further improve the performance of whole viscidity micropump.
Drawings
Fig. 1 is a schematic structural view of the viscous micro-pump of the present invention.
Fig. 2 is a schematic structural diagram of the L-shaped adapter plate of the present invention.
Fig. 3 is a schematic structural diagram of the fluid driving chip of the present invention.
Fig. 4 is a schematic structural diagram of the microstructure layer of the present invention.
Fig. 5 is a schematic structural diagram of the lower ITO glass layer of the present invention.
FIG. 6 is a schematic diagram showing the distribution of algal cells in the microchannel of the fluid driver chip according to the present invention.
FIG. 7 shows the fluid driving chip of the present invention
And (5) finite element simulation results.
Fig. 8 is a finite element simulation result of the algae cell driving fluid rotating in opposite directions in the microchannel according to the present invention.
Detailed Description
The embodiments of the present invention will be further explained with reference to the drawings.
As shown in fig. 1-6, an algae cell-based viscous micropump includes a fluid driving chip 1, an L-shaped adapter plate 2, a three-dimensional nano moving platform 3, a microscope 4, a CCD5, a high-precision Z-direction displacement stage 6, a projector 7, a focusing objective 8, a signal generator 9 and a computer 10, wherein the fluid driving chip 1 is horizontally disposed, a fluid inlet and a fluid outlet are disposed on the upper surface of the fluid driving chip 1 at intervals from left to right, a micro-pipe 11 disposed between the fluid inlet and the fluid outlet is disposed in the fluid driving chip 1 along the left-right direction, the fluid inlet is connected to the left end of the micro-pipe 11, the fluid outlet is connected to the right end of the micro-pipe 11, the L-shaped adapter plate 2 includes a horizontal plate and a vertical plate, the left side of the horizontal plate of the L-shaped adapter plate 2 is fixedly connected to the lower side of the vertical plate, the left side of the vertical plate of the L-shaped adapter plate 2 is fixedly connected to the three-dimensional nano moving platform 3, the middle of the horizontal plate of the L-shaped adapter plate 2 is provided with a rectangular fluid driving chip 12 having an upper portion, and a rectangular fluid driving chip 12 having a size matching the same size as the rectangular fluid driving chip 1, the microscope 4 is arranged right above the fluid driving chip 1, the CCD5 is fixedly arranged on the microscope 4, a lens barrel of the microscope 4 is fixedly connected with the high-precision Z-direction displacement table 6, the projector 7 is arranged right below the fluid driving chip 1, a lens of the projector 7 is arranged on the top of the projector 7 and irradiates upwards, the focusing objective 8 is arranged on the upper portion of the lens of the projector 7, the light path of the focusing objective 8 is overlapped with that of the microscope 4, the signal generator 9 is electrically connected with the fluid driving chip 1, and the computer 10 is respectively in signal connection with the CCD5 and the projector 7.
The fluid driving chip 1 comprises an upper ITO glass layer 13 and a lower ITO glass layer 14, a micro-structural layer 15 is arranged between the upper ITO glass layer 13 and the lower ITO glass layer 14, the length directions of the upper ITO glass layer 13 and the lower ITO glass layer 14 are both arranged along the left-right direction, the upper ITO glass layer 13 and the lower ITO glass layer 14 are both rectangular sheet structures with the length of 25mm and the width of 20mm, the thickness of the upper ITO glass layer 13 and the thickness of the lower ITO glass layer 14 are both 1mm, two first through holes 16 which are 0.5mm in diameter and are through up and down are arranged on the upper ITO glass layer 13 at intervals from left to right, the first through hole 16 on the left side is a fluid inlet, the first through hole 16 on the right side is a fluid outlet, the micro-structural layer 15 is a square sheet structure with the side length of 20mm, the thickness of the micro-structural layer 15 is 0.02-0.1mm, a rectangular long hole 17 which is 10mm in length, 0.1mm in width and is through up and down is arranged in the left-right direction in the middle of the micro-structural layer 15, two vertically-transparent second through holes 18 with the diameter of 0.5mm are arranged on the micro-structural layer 15 at left and right intervals, the rectangular long hole 17 is positioned between the two second through holes 18, the left end of the rectangular long hole 17 is communicated with the left second through hole 18, the right end of the rectangular long hole 17 is communicated with the right second through hole 18, the lower surface of the upper ITO glass layer 13 and the upper surface of the lower ITO glass layer 14 are conductive surfaces, the signal generator 9 is respectively and electrically connected with the two conductive surfaces through conducting wires, a hydrogenated amorphous silicon layer 19 is coated on the conductive surface of the lower ITO glass layer 14 in a spinning mode, the area of the hydrogenated amorphous silicon layer 19 is a square area with the side length of 20mm, the thickness of the hydrogenated amorphous silicon layer 19 is 1 mu m, the right side of the hydrogenated amorphous silicon layer 19 is flush with the right side of the upper surface of the lower ITO glass layer 14, the conductive surface of the upper ITO glass layer 13 is bonded with the upper surface of the micro-structural layer 15, the left side of the lower surface of the upper ITO glass layer 13 is flush with the left side of the upper surface of the micro-structural layer 15, the upper surface of the hydrogenated amorphous silicon layer 19 is bonded with the lower surface of the microstructure layer 15, the microstructure layer 15 and the hydrogenated amorphous silicon layer 19 are aligned up and down, the two first through holes 16 are respectively aligned up and down with the two second through holes 18, and the rectangular long hole 17 is enclosed into the micro-pipeline 11 with the length of 10mm, the width of 0.1mm and the height of 0.02-0.1mm after the upper ITO glass layer 13, the microstructure layer 15 and the lower ITO glass layer 14 are bonded.
The L-shaped adapter plate 2 is an aluminum plate, the width of the L-shaped adapter plate 2 is 50mm, the thickness of the L-shaped adapter plate 2 is 2mm, the height of a vertical plate of the L-shaped adapter plate 2 is 50mm, the length of a horizontal plate of the L-shaped adapter plate 2 is 200mm, the upper groove of the stepped rectangular groove 12 is 25mm long, 20mm wide and 0.5mm thick, and the lower groove of the stepped rectangular groove 12 is 24mm long, 18mm wide and 1.5mm thick.
The three-dimensional nanometer mobile platform 3, the microscope 4, the CCD5, the high-precision Z-direction displacement platform 6, the projector 7, the focusing objective lens 8, the signal generator 9 and the computer 10 are all conventional technologies, and specific structures and working principles are not repeated.
An operation method of a viscous micro-pump based on algae cells specifically comprises the following steps:
firstly, assembling and connecting a viscous micropump;
(II) slowly injecting the treated algae cell solution into the micro-pipeline 11 in the fluid driving chip 1 from the fluid inlet;
thirdly, drawing a plurality of light spot images on a computer 10 by using flash drawing software, wherein each light spot image is projected upwards through a projector 7, converged through a focusing objective 8 and projected upwards through a gap of a stepped rectangular groove 12 to the lower surface of the fluid driving chip 1;
fourthly, adjusting the three-dimensional nano moving platform 3 and the high-precision Z-direction displacement platform 6 to focus the focal plane of the focusing objective lens 8 and the focal plane of the microscope 4 on the micro-pipeline 11 in the fluid driving chip 1;
fifthly, adjusting the size of the light spot drawn in the step (three), adjusting the size of the light spot finally projected on the fluid driving chip 1 to be 10-15 mu m, and then starting the signal generator 9, wherein the signal generator 9 generates a sinusoidal signal with the frequency of 60KHz and the peak value of 6V and acts on the fluid driving chip 1 through a lead;
capturing autorotation algae cells in the micro-pipeline 11 by utilizing the projected light spots and moving the algae cells for arrangement;
and (seventh), removing the redundant algae cells from the microchannel 11, and turning off the signal generator 9 and the projector 7, so that the algae cells rotating in the microchannel 11 drive the fluid to flow in the microchannel 11 from the fluid inlet to the fluid outlet.
The step (II) is specifically as follows: collecting 1ml of algae cell solution, centrifuging at 3000rpm in a centrifuge for 6min, removing supernatant, and weighingAdding deionized water, and adjusting the concentration of the centrifuged algae cell solution to 1 × 10 5 cells/ml, and then the treated algal cell solution is injected from the fluid inlet into the microchannel 11 within the fluid driven chip 1.
The reason why the spot size finally projected onto the fluid driving chip 1 is adjusted to 10 to 15 μm in the step (five) is as follows: the shape of the algae cells is elliptical or oval, the size of the algae cells is about 10 mu m, and the size of the light spot is adjusted to be equivalent to that of the algae cells, so that the capture operation of single algae cells is more convenient to realize;
in the step (V), the sinusoidal signal generated by the signal generator 9 acts on the conductive surface of the upper ITO glass layer 13 and the conductive surface of the lower ITO glass layer 14 through the conducting wires respectively.
The step (six) is specifically as follows: feeding back the image in the fluid driving chip 1 observed by the microscope 4 to the computer 10 through the CCD5, moving each light spot drawn by flash drawing software on the computer 10 by combining the fed-back image, further moving the light spots projected on the fluid driving chip 1, respectively capturing the autorotation algae cells in the micro-pipeline 11 by each light spot, arranging each clockwise rotation algae cell at the position close to the front side pipeline wall in the micro-pipeline 11 at a left-right interval, and arranging each counterclockwise rotation algae cell at the position close to the rear side pipeline wall in the micro-pipeline 11 at a left-right interval.
The rotating mechanism of the algae cells in the step (VI) is as follows: the front end of the body of the algae cell is provided with two flagella, the two flagella of the algae cell belong to a linear motor and can only generate a certain degree of bending swing, the bending swing of the two flagella of the algae cell is generated by relative sliding caused by the conformational change of dynein between adjacent microtubules, when both the two flagella are in a free state, the algae cell shows a random swimming motion form, and when only a single flagella is in the free swing state, the algae cell shows a counterclockwise rotation or clockwise rotation motion form.
The mechanism of capturing and moving algae cells by the light spots in the step (six) is as follows: when the ITO glass layer 14 is not irradiated by the light spot, the conductivity of the hydrogenated amorphous silicon layer 19 is very low, and the hydrogenated amorphous silicon layer 19 can be regarded as an insulating layer, the voltage loaded on the fluid driving chip 1 is basically applied to the hydrogenated amorphous silicon layer 19, when the light spot is irradiated on the hydrogenated amorphous silicon layer 19, due to electron-hole pairs generated by the light spot, the conductivity of the hydrogenated amorphous silicon layer 19 in the spot area is increased sharply, the hydrogenated amorphous silicon layer 19 in the spot area is equivalent to a conductor, most of the voltage falls on the microstructure layer 15, so that a non-uniform electric field is generated in the microstructure layer 15 in the spot area and the spot-free area, the algae cells are polarized in the non-uniform electric field area and are further subjected to the light-induced dielectrophoresis force, and the expression of the light-induced dielectrophoresis force applied to the algae cells is as follows:
in the formula
Is the dielectric constant of the solution medium,
is the diameter of the algal cells and is,
is a C-M factor
The real part of (a) is,
a gradient that is the square of the root mean square value of the electric field;
when the signal generator 9 generates a sinusoidal signal with a frequency of 60KHz and a peak-to-peak value of 6V and acts on the conductive surface of the upper ITO glass layer 13 and the conductive surface of the lower ITO glass layer 14 of the fluid driving chip 1 through the wires, the algal cells receive the light-induced dielectrophoresis force of the spot-free area pointing to the spot area, and the light-induced dielectrophoresis force is greater than the motility of the algal cells themselves, so that the spots can capture and move the algal cells.
The step (VII) is specifically as follows: locking the arranged algae cells by using each light spot, and injecting deionized water into the micro-pipeline 11 from the fluid inlet at a certain flow rate, wherein the flow rate of the deionized water meets the following conditions: the fluid drag force on the algae cells locked by the light spots needs to be smaller than the light-induced dielectrophoresis force on the algae cells, so that the rest algae cells which are not locked by the light spots are washed away from the microchannel 11 by the deionized water, and then the signal generator 9 and the projector 7 are closed, so that the algae cells rotating in the microchannel 11 drive the fluid to flow from the fluid inlet to the fluid outlet.
The mechanism that the algae cells rotating in the micro-pipe 11 in the step (seven) drive the fluid to flow in the micro-pipe 11 from the fluid inlet to the fluid outlet is as follows: because the gap between the autorotation algae cell and the inner rear side pipeline wall of the micro pipeline 11 is different from the gap between the autorotation algae cell and the inner front side pipeline wall of the micro pipeline 11, the viscous resistance of the fluid on two sides of the algae cell is different in the rotating process of the algae cell, so that the algae cell can generate a net flow to the fluid when rotating, and the fluid can flow to the right end from the left end of the micro pipeline 11: the counter-clockwise rotating row of algae cells near the rear wall of the microchannel 11 drives the fluid to flow from the fluid inlet to the fluid outlet in the microchannel 11, the counter-clockwise rotating row of algae cells near the front wall of the microchannel 11 also drives the fluid to flow from the fluid inlet to the fluid outlet in the microchannel 11, and the driving effects of the algae cells rotating in opposite directions on the two sides are the same.
FIG. 7 shows that when a sinusoidal signal of 60KHz and 6V peak value is applied to the conductive surface of the upper ITO glass layer 13 and the conductive surface of the lower ITO glass layer 14 of the fluid driving chip 1, and the spot size projected onto the surface of the hydrogenated amorphous silicon layer 19 is 10 μm, the fluid driving chip 1 has a light spot size
Finite element simulation results, the magnitude and direction of which represent the magnitude and direction of light-induced dielectrophoretic force, black arrows in the simulation results
And the direction of most arrows is pointed to the facula area from the facula-free area from the simulation result.
FIG. 8 shows the result of finite element simulation of the fluid driving chip 1 driving the fluid by the algae cells rotating counterclockwise at 6.28rad/s near the inner rear side of the micro duct 11 and the algae cells rotating clockwise at 6.28rad/s near the inner front side of the micro duct 11, and it can be seen that the driving effect generated by the algae cells rotating in opposite directions at both sides is the same.
Although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that the above embodiments are only used for illustration and not for limitation of the technical solutions of the present invention; the present invention may be modified or substituted with equivalents without departing from the spirit and scope of the invention, which should be construed as being limited only by the claims.
Claims (3)
1. An algal cell based viscous micropump, characterized by: the device comprises a fluid driving chip, an L-shaped adapter plate, a three-dimensional nano moving platform, a microscope, a CCD, a high-precision Z-direction displacement table, a projector, a focusing objective lens, a signal generator and a computer, wherein the fluid driving chip is horizontally arranged, a fluid inlet and a fluid outlet are arranged on the upper surface of the fluid driving chip at intervals from left to right, a micro pipeline positioned between the fluid inlet and the fluid outlet is arranged in the fluid driving chip along the left-right direction, the fluid inlet is connected with the left end of the micro pipeline, the fluid outlet is connected with the right end of the micro pipeline, the L-shaped adapter plate comprises a horizontal plate and a vertical plate, the left side of the horizontal plate of the L-shaped adapter plate is fixedly connected with the lower side of the vertical plate in an integrated forming way, and the left side of the vertical plate of the L-shaped adapter plate is fixedly connected on the three-dimensional nano moving platform, the horizontal plate right side middle part of L type keysets is equipped with the upside, downside and right side are all uncovered and big-end-up's ladder rectangular channel, the fluid drive chip is the same with the size in the upper portion groove of ladder rectangular channel, the fluid drive chip matching inlays to be established in the upper portion groove of ladder rectangular channel, the microscope sets up directly over the fluid drive chip, CCD fixed mounting is on microscopical upper portion, microscopical lens cone and high-accuracy Z are to displacement platform fixed connection, the projector sets up directly under the fluid drive chip, the camera lens setting of projector is at the top of projector and upwards shine, focus objective sets up on the camera lens upper portion of projector, focus objective's light path and microscope's light path coincidence, signal generator is connected with the fluid drive chip electricity, the computer is respectively with CCD and projector signal connection.
2. The algal cell based viscous micropump of claim 1, wherein: the fluid driving chip comprises an upper ITO glass layer and a lower ITO glass layer, a micro-structural layer is arranged between the upper ITO glass layer and the lower ITO glass layer, the length directions of the upper ITO glass layer and the lower ITO glass layer are both arranged along the left-right direction, the upper ITO glass layer and the lower ITO glass layer are both rectangular sheet structures with the length of 25mm and the width of 20mm, the thickness of the upper ITO glass layer and the thickness of the lower ITO glass layer are both 1mm, two first through holes with the diameter of 0.5mm and through up and down are arranged on the upper ITO glass layer at a left-right interval, the first through hole on the left side is a fluid inlet, the first through hole on the right side is a fluid outlet, the micro-structural layer is a square sheet structure with the side length of 20mm, the thickness of the micro-structural layer is 0.02-0.1mm, a rectangular long hole with the length of 10mm and the width of 0.1mm and through up and down is arranged in the middle of the left-right direction of the micro-structural layer, two second through holes with the diameter of 0.5mm and through up and down are arranged on the left-right interval of the micro-structural layer, the rectangular long hole is positioned between the two second through holes, the left end of the rectangular long hole is communicated with the second through hole on the left side, the right end of the rectangular long hole is communicated with the second through hole on the right side, the lower surface of the upper ITO glass layer and the upper surface of the lower ITO glass layer are conductive surfaces, the signal generator is respectively electrically connected with the two conductive surfaces through a wire, a hydrogenated amorphous silicon layer is spirally coated on the conductive surface of the lower ITO glass layer, the area of the hydrogenated amorphous silicon layer is a square area with the side length of 20mm, the thickness of the hydrogenated amorphous silicon layer is 1 mu m, the right side edge of the hydrogenated amorphous silicon layer is flush with the right side edge of the upper surface of the lower ITO glass layer, the conductive surface of the upper ITO glass layer is bonded with the upper surface of the microstructure layer, the left side edge of the lower surface of the upper ITO glass layer is flush with the left side edge of the upper surface of the microstructure layer, the upper surface of the hydrogenated amorphous layer is bonded with the lower surface of the microstructure layer, and the microstructure layer is aligned with the hydrogenated amorphous layer up and down, the two first through holes are respectively aligned with the two second through holes up and down correspondingly, and the rectangular long hole is surrounded into a micro pipeline with the length of 10mm, the width of 0.1mm and the height of 0.02-0.1mm after the upper ITO glass layer, the micro-structural layer and the lower ITO glass layer are bonded.
3. The algal cell based viscosity micropump of claim 1 or 2, wherein: the L-shaped adapter plate is an aluminum plate, the width of the L-shaped adapter plate is 50mm, the thickness of the L-shaped adapter plate is 2mm, the height of the vertical plate of the L-shaped adapter plate is 50mm, the length of the horizontal plate of the L-shaped adapter plate is 200mm, the size of the upper groove of the stepped rectangular groove is 25mm long, 20mm wide and 0.5mm thick, and the size of the lower groove of the stepped rectangular groove is 24mm long, 18mm wide and 1.5mm thick.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122967603.7U CN218339828U (en) | 2021-11-30 | 2021-11-30 | Viscous micropump based on algae cells |
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| CN202122967603.7U CN218339828U (en) | 2021-11-30 | 2021-11-30 | Viscous micropump based on algae cells |
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