CN218290948U - Stirring bioreactor for preparing GOS-rich low-lactose milk - Google Patents

Stirring bioreactor for preparing GOS-rich low-lactose milk Download PDF

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Publication number
CN218290948U
CN218290948U CN202222370336.XU CN202222370336U CN218290948U CN 218290948 U CN218290948 U CN 218290948U CN 202222370336 U CN202222370336 U CN 202222370336U CN 218290948 U CN218290948 U CN 218290948U
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stirring
enzymolysis tank
stirring rod
enzymolysis
tank
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葛武鹏
原卉卉
杨孝成
张艳
赵丽丽
王爽爽
丁一
张闹
高秦艺
谢玉霞
李帅
张永金
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Northwest A&F University
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Northwest A&F University
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Abstract

The utility model discloses a preparation is rich in stirring formula bioreactor of GOS low lactose milk belongs to the biotechnology field, including first enzymolysis tank, second enzymolysis tank and storage tank, the structure of first enzymolysis tank and second enzymolysis tank is the same, be equipped with pan feeding mouth and outlet pipe on the first enzymolysis tank, first enzymolysis tank and second enzymolysis tank pass through the pipe connection, the lower extreme of second enzymolysis tank passes through the pipe connection storage tank, be equipped with agitating unit, stirring folding device and stirring belt cleaning device on the first enzymolysis tank, agitating unit set up on first enzymolysis tank and with first enzymolysis tank normal running fit, stirring folding device sets up and serves in agitating unit's rotation, stirring belt cleaning device is located the interior top of first enzymolysis tank. The utility model discloses a collapsible shrink mechanism, when need not carrying out the enzymolysis to lactose, but rapid folding stirring vane reaches the effect of enzyme and substrate separation, avoids the enzymolysis excessive.

Description

Stirring bioreactor for preparing GOS-rich low-lactose milk
Technical Field
The utility model belongs to the technical field of it is biological, the body relates to a stirring formula bioreactor of low lactose milk of preparation richly containing GOS.
Background
The dairy product is rich in nutrition, and is one of the most ideal natural foods given to human beings in nature. However, lactose intolerance, a worldwide problem, has become an important factor that limits the growth of dairy consumption. A great deal of research is carried out on scholars at home and abroad, the most direct and effective method for solving the problem is to select the dairy products without lactose or with low lactose content for drinking, and most dairy enterprises produce low-lactose dairy products by directly adding lactase into the milk. However, the method has the defects that lactase cannot be recovered, the product quality is influenced, the reaction time cannot be controlled, the lactase dosage is large, the production cost is high and the like, and the immobilized lactase has the advantages of higher stability, easier control of the catalytic reaction process, repeated use of enzyme, cost saving, no exogenous enzyme contained in the product, improvement of the product quality and the like, and is expected to solve the problems; the use of the patent enzyme Nurica can produce abundant galacto-oligosaccharides (GOS) while degrading lactose in milk, is beneficial to proliferation of beneficial bacteria in human intestinal tracts, and has the effects of preventing decayed teeth, improving mineral absorption, improving serum lipid, being beneficial to generation of B vitamins, preventing cancer and lowering blood pressure. When the GOS content reaches the maximum value, the enzymes begin to be decomposed into galactose and glucose, the lactose decomposition is not complete, the enzyme and the milk are quickly separated in time, and other lactases are used for carrying out the residual lactose decomposition again, which is a key factor for ensuring the stable GOS content and the low lactose content, so that a multi-enzyme continuous reactor for preparing the stirring type bioreactor rich in GOS low lactose milk can quickly separate the enzymes is needed.
At present, the common immobilized enzyme reactors generally comprise packed bed reactors, stirred tank reactors and the like, and have the defects of insufficient and uniform contact between substrates and enzymes, slow enzyme separation rate, easy damage of carriers by blade shearing force and the like, and particularly, reactors for multi-enzyme stepwise enzymolysis are rare and still need to be further improved.
Therefore, it is desirable to provide a continuous stirring bioreactor for preparing low lactose milk rich in GOS, which can simultaneously perform multi-stage enzymatic hydrolysis and rapid separation of enzyme from product.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a weak point to prior art, provide a stirring formula bioreactor of low lactose milk of preparation richly containing GOS for solve the problem among the prior art.
The embodiment of the utility model provides an adopt following technical scheme: the utility model provides a preparation is rich in stirring formula bioreactor that contains GOS low lactose milk, first enzymolysis tank, second enzymolysis tank and storage tank of its characterized in that, first enzymolysis tank and second enzymolysis tank's structure is the same, be equipped with pan feeding mouth and outlet pipe on the first enzymolysis tank, pass through pipe connection between first enzymolysis tank and the second enzymolysis tank, the lower extreme of second enzymolysis tank passes through the pipe connection storage tank, be equipped with agitating unit, stirring folding device and stirring belt cleaning device on the first enzymolysis tank, agitating unit set up on first enzymolysis tank and with first enzymolysis tank normal running fit, stirring folding device sets up and serves at agitating unit's rotation, stirring belt cleaning device is located the interior top of first enzymolysis tank, be equipped with the heating pipe in the first enzymolysis tank, first enzymolysis tank is equipped with temperature sensor.
Further, agitating unit includes motor support, agitator motor and (mixing) shaft, motor support is located the top of first enzymolysis tank, agitator motor is located motor support, the (mixing) shaft is located first enzymolysis tank and the top of (mixing) shaft is connected with agitator motor's spindle drive.
Further, the stirring folding device comprises a driving motor, a first gear, a second gear, a driving lead screw, a threaded shaft, a first threaded shaft sleeve, a second threaded shaft sleeve, a first stirring rod, a second stirring rod and a third stirring rod, wherein the driving motor is positioned in the stirring shaft, the first gear is positioned on a main shaft of the driving motor, the threaded shaft is rotatably connected with the stirring shaft, the second gear is positioned on the threaded shaft and meshed with the first gear, the first stirring rod is slidably connected on the stirring shaft, the first threaded shaft sleeve is rotatably connected on the threaded shaft and the first threaded shaft sleeve is rotatably connected with the first stirring rod, the second stirring rod is slidably connected on the first stirring rod, a threaded seat in threaded connection with the outer wall of the first threaded shaft sleeve is arranged at the end part of the second stirring rod, the third stirring rod is slidably matched with the second stirring rod, the second threaded shaft sleeve is in threaded connection with the first threaded shaft sleeve, a threaded connection thread section is arranged on the inner wall of the third stirring rod, a thread section is arranged between the first stirring rod and the second stirring rod, and a third stirring rod limiting block is arranged on the inner wall of the third stirring rod.
Furthermore, each stirring blade is provided with a plurality of enzyme carrying holes, and lactase is arranged in the enzyme carrying holes.
Further, stirring belt cleaning device includes ring pipe and a plurality of nozzle, the ring pipe is located the interior top of first enzymolysis tank, and is a plurality of the nozzle is equidistant to be set up on the ring pipe, be equipped with the filler that extends to outside the first enzymolysis tank on the ring pipe.
The embodiment of the utility model provides an above-mentioned at least one technical scheme who adopts can reach following beneficial effect:
one of which, the utility model discloses a collapsible shrink mechanism, when need not carrying out the enzymolysis to lactose (when GOS content reaches the biggest promptly), but quick folding stirring vane rises to the top of first enzymolysis tank, reaches enzyme and substrate quickly separating's effect, thereby avoids the substrate to lead to the enzymolysis excessively from first enzymolysis tank bottom outflow time overlength.
And secondly, the utility model discloses set up stirring belt cleaning device in, after the enzymolysis was accomplished, will start stirring belt cleaning device, the clear water passes through water pressure and passes through the annular tube from the filler after, washs first enzymolysis tank through shower nozzle blowout clear water to next time use.
Third, the utility model discloses set up two enzymolysis tank, accomplish the back to the lactose enzymolysis in the first enzymolysis tank, if accord with the production requirement, can carry out enzymolysis on next step with the direct delivery of substrate to the second enzymolysis tank, if not conform to then put into the material with stirring vane and carry out enzymolysis again, can carry out multistage enzymolysis.
Drawings
The accompanying drawings, which are described herein, serve to provide a further understanding of the invention and constitute a part of this specification, and the exemplary embodiments and descriptions thereof are provided for explaining the invention without unduly limiting it. In the drawings:
fig. 1 is a schematic perspective view of the present invention;
fig. 2 is a partial cross-sectional view of the present invention;
FIG. 3 is a schematic view of a partial three-dimensional structure of the present invention;
fig. 4 is a schematic view of the stirring and folding device of the present invention in a contracted state;
fig. 5 is a schematic view of the stirring and folding device of the present invention in an unfolded state;
fig. 6 is a schematic perspective view of the stirring device and the stirring folding device of the present invention;
reference numerals
The device comprises a first enzymolysis tank 1, a second enzymolysis tank 2, a storage tank 3, a stirring device 4, a motor support 41, a stirring motor 42, a stirring shaft 43, a stirring and folding device 5, a driving motor 51, a first gear 52, a second gear 53, a threaded shaft 54, a first threaded shaft sleeve 55, a second threaded shaft sleeve 56, a first stirring rod 57, a second stirring rod 59, a third stirring rod 591, a threaded seat 592, a stirring blade 593, an enzyme carrying hole 594, a stirring and cleaning device 6, a water filling port 61, an annular pipe 62, a nozzle 63, a material inlet 7, a water outlet pipe 8 and a temperature sensor 9.
Detailed Description
To make the purpose, technical solution and advantages of the present invention clearer, the following description will be given with reference to the embodiments of the present invention and the accompanying drawings, in which the technical solution of the present invention is clearly and completely described. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.
Referring to fig. 1 to 6, the embodiment of the utility model provides a preparation is rich in stirring formula bioreactor of GOS low lactose milk, including first enzymolysis tank 1, second enzymolysis tank 2 and storage tank 3, first enzymolysis tank 1 is the same with the structure of second enzymolysis tank 2, be equipped with pan feeding mouth 7 and outlet pipe 8 on the first enzymolysis tank 1, through the pipe connection between first enzymolysis tank 1 and the second enzymolysis tank 2, the lower extreme of second enzymolysis tank 2 passes through pipe connection storage tank 3, be equipped with agitating unit 4, stirring folding device 5 and stirring belt cleaning device 6 on the first enzymolysis tank 1, agitating unit 4 sets up on first enzymolysis tank 1 and with 1 normal running fit of first enzymolysis tank, stirring folding device 5 sets up and holds at agitating unit 4's rotation, stirring belt cleaning device 6 is located the interior top of first enzymolysis tank 1, be equipped with the heating pipe in the first enzymolysis tank 1, first enzymolysis tank 1 is equipped with temperature sensor 9, and temperature sensor 9's model is 8978-236.
When the milk enzymolysis tank is used, milk enters from a feeding port 7 of a first enzymolysis tank 1, the milk is heated by a heating pipe after being added to a certain amount, a temperature sensor 9 monitors the temperature in the first enzymolysis tank 1, the milk stops heating and keeps constant temperature after reaching the required temperature, a stirring and folding device 5 extends to a specified position in the first enzymolysis tank 1, the milk is stirred and enzymolyzed through a stirring device 4, the milk enters a second enzymolysis tank 2 through a pipeline after the enzymolysis is completed to continue secondary enzymolysis, the milk enters a storage tank 3 through a pipeline between the second enzymolysis tank 2 and the storage tank 3 to be stored, and after the processing is completed, the inside of the enzymolysis tank is cleaned through a stirring and cleaning device 6 so as to be used for the next time, and the cleaned water is discharged outwards through a water outlet pipe 8; after the lactose enzymolysis is completed in the first enzymolysis tank 1, if the production requirements are met, the substrate can be directly conveyed to the second enzymolysis tank 2 for next enzymolysis, and if the substrate does not meet the production requirements, the stirring blade 593 is placed into the material for re-enzymolysis. The number of the required tank bodies can be flexibly designed according to actual production requirements.
Preferably, the stirring device 4 comprises a motor support 41, a stirring motor 42 and a stirring shaft 43, the motor support 41 is located at the top of the first enzymolysis tank 1, the stirring motor 42 is located on the motor support 41, the stirring shaft 43 is located in the first enzymolysis tank 1, and the top of the stirring shaft 43 is in transmission connection with a main shaft of the stirring motor 42; when enzymolysis milk, agitator motor 42 main shaft drives (mixing) shaft 43 and rotates, and then makes stirring folding device 5 rotatory stirring milk for milk and lactase carry out abundant contact and mix, so that carry out the enzymolysis to lactose.
Preferably, the stirring folding device 5 comprises a driving motor 51, a first gear 52, a second gear 53, a threaded shaft 54, a first threaded shaft sleeve 55, a second threaded shaft sleeve 56, a first stirring rod 57, a second stirring rod 59 and a third stirring rod 591, wherein the driving motor 51 is positioned in the stirring shaft 43, the first gear 52 is positioned on a main shaft of the driving motor 51, the threaded shaft 54 is rotatably connected with the stirring shaft 43, the second gear 53 is positioned on the threaded shaft 54 and is meshed with the first gear 52, the first stirring rod 57 is slidably connected with the stirring shaft 43, the first threaded shaft sleeve 55 is threadedly connected with the threaded shaft 54, the first threaded shaft sleeve 55 is rotatably connected with the first stirring rod 57, the second stirring rod 59 is slidably connected with the first stirring rod 57, the end of the second stirring rod 59 is provided with a threaded seat 592 in threaded connection with the outer wall of the first threaded shaft sleeve 55, the third stirring rod 591 is slidably matched with the second stirring rod 59, the second threaded shaft sleeve 56 is in threaded connection with the first threaded shaft sleeve 55, the third stirring rod 591 and a third stirring rod 591 is provided with a second stirring rod 591, and a third stirring rod 591, a third stirring rod 59 and a third stirring rod 591 is provided with a second stirring rod capable of preventing a stirring rod 593 and a stirring rod 591, a second stirring rod 59, a second stirring rod 591, a third stirring rod 59, a third stirring rod and a stirring rod 591 are provided between the stirring rod, a stirring rod 59, a stirring rod and a stirring rod 59, a stirring rod and a stirring rod 591, a stirring rod and a stirring rod, a stirring rod 59, a stirring rod and a stirring rod 591 are provided; when the milk is enzymolyzed, the driving motor 51 drives the first gear 52 on the main shaft to rotate, so as to drive the second gear 53 meshed with the first gear 52 to rotate with the threaded shaft 54, then the first stirring rod 57 is driven to extend, the threaded shaft 54 drives the first threaded shaft sleeve 55 and the threaded seat 592 to rotate, so as to drive the second stirring rod 59 to extend, meanwhile, the rotating threaded seat 592 and the second threaded shaft sleeve 56 rotate, so as to drive the third stirring rod 591 to extend, a plurality of stirring blades 593 are extended into the first enzymolysis tank 1, the stirring device 4 rotates to drive the stirring blades 593 to rotate in the milk for enzymolysis, when the lactose is not required to be enzymolyzed (namely when the GOS content reaches the maximum), the stirring blades 593 can be folded fast to rise to the top of the first enzymolysis tank 1, the effect of fast separation of enzyme substrates is achieved, and the phenomenon that the substrates flow out from the bottom of the first enzymolysis tank 1 for a long time is avoided, so as to cause excessive.
Preferably, each stirring blade 593 is provided with a plurality of enzyme carrying holes 594, and lactase is arranged in each enzyme carrying hole 594; lactase can rapidly react with lactose to carry out enzymolysis operation on lactose.
Preferably, the stirring and cleaning device 6 comprises an annular pipe 62 and a plurality of nozzles 63, the annular pipe 62 is located at the inner top of the first enzymolysis tank 1, the nozzles 63 are arranged on the annular pipe 62 at equal intervals, and the annular pipe 62 is provided with a water filling port 61 extending to the outside of the first enzymolysis tank 1; after the enzymolysis is finished, the stirring and cleaning device 6 is started, and the clear water passes through the water pressure and passes through the annular pipe 62 from the water filling port 61, and then is sprayed out through the spray head to clean the first enzymolysis tank 1, so as to be used for the next time.
The above description is only an example of the present invention, and is not intended to limit the present invention. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims (5)

1. The utility model provides a preparation is rich in stirring formula bioreactor that contains GOS low lactose milk, its characterized in that, first enzymolysis tank (1), second enzymolysis tank (2) and storage tank (3), the structure of first enzymolysis tank (1) and second enzymolysis tank (2) is the same, be equipped with pan feeding mouth (7) and outlet pipe (8) on first enzymolysis tank (1), through the pipe connection between first enzymolysis tank (1) and the second enzymolysis tank (2), the lower extreme of second enzymolysis tank (2) passes through pipe connection storage tank (3), be equipped with agitating unit (4), stirring folding device (5) and stirring belt cleaning device (6) on first enzymolysis tank (1), agitating unit (4) set up on first enzymolysis tank (1) and with first enzymolysis tank (1) normal running fit, stirring folding device (5) set up and serve in the rotation of agitating unit (4), stirring belt cleaning device (6) are located the interior top of first enzymolysis tank (1), be equipped with heating pipe (1) in the first enzymolysis tank (9) enzymolysis tank (1) enzymolysis temperature sensor (9).
2. An agitated bioreactor for producing GOS-enriched low lactose milk according to claim 1, wherein: agitating unit (4) include motor support (41), agitator motor (42) and (mixing) shaft (43), motor support (41) are located the top of first enzymolysis tank (1), agitator motor (42) are located motor support (41), agitator shaft (43) are located first enzymolysis tank (1) and the top of (mixing) shaft (43) is connected with the spindle drive of agitator motor (42).
3. An agitated bioreactor for producing GOS-enriched low lactose milk according to claim 2, wherein: the stirring folding device (5) comprises a driving motor (51), a first gear (52), a second gear (53), a threaded shaft (54), a first threaded shaft sleeve (55), a second threaded shaft sleeve (56), a first stirring rod (57), a second stirring rod (59) and a third stirring rod (591), wherein the driving motor (51) is positioned in the stirring shaft (43), the first gear (52) is positioned on a main shaft of the driving motor (51), the threaded shaft (54) is rotatably connected with the stirring shaft (43), the second gear (53) is positioned on the threaded shaft (54) and is meshed with the first gear (52), the first stirring rod (57) is slidably connected on the stirring shaft (43), the first threaded shaft sleeve (55) is in threaded connection with the threaded shaft (54), the first threaded shaft sleeve (55) is rotatably connected with the first stirring rod (57), the second stirring rod (59) is slidably connected on the first stirring rod (57), the end part of the second stirring rod (59) is provided with a threaded shaft sleeve (56), and the second stirring rod (592) is in threaded connection with a second stirring rod (591) and is in threaded connection with a second stirring rod (592) and a second stirring rod (591) seat, and a limiting block is arranged among the first stirring rod (57), the second stirring rod (59) and the third stirring rod (591), and stirring blades (593) are arranged on the first stirring rod (57), the second stirring rod (59) and the third stirring rod (591).
4. An agitated bioreactor for producing GOS-enriched low lactose milk according to claim 1, wherein: stirring belt cleaning device (6) include annular tube (62) and a plurality of nozzle (63), annular tube (62) are located the interior top of first enzymolysis tank (1), and are a plurality of nozzle (63) equidistant setting is on annular tube (62), be equipped with on annular tube (62) and extend to outer filler (61) of first enzymolysis tank (1).
5. An agitated bioreactor for producing GOS-enriched low lactose milk according to claim 3, wherein: each stirring blade (593) is provided with a plurality of enzyme carrying holes (594), and lactase is arranged in each enzyme carrying hole (594).
CN202222370336.XU 2022-09-06 2022-09-06 Stirring bioreactor for preparing GOS-rich low-lactose milk Active CN218290948U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202222370336.XU CN218290948U (en) 2022-09-06 2022-09-06 Stirring bioreactor for preparing GOS-rich low-lactose milk

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202222370336.XU CN218290948U (en) 2022-09-06 2022-09-06 Stirring bioreactor for preparing GOS-rich low-lactose milk

Publications (1)

Publication Number Publication Date
CN218290948U true CN218290948U (en) 2023-01-13

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