CN218202871U

CN218202871U - Stem cell culture perfusion device

Info

Publication number: CN218202871U
Application number: CN202222498440.7U
Authority: CN
Inventors: 沈健; 葛永; 刘正明; 杨淑青
Original assignee: Jiangsu Mengbili Biological Technology Co ltd
Current assignee: Jiangsu Mengbili Biological Technology Co ltd
Priority date: 2022-09-21
Filing date: 2022-09-21
Publication date: 2023-01-03
Anticipated expiration: 2032-09-21

Abstract

The utility model provides a stem cell culture perfusion device, including cultivateing box and a plurality of filling subassembly, from last cultivation storehouse and the filling storehouse of down being equipped with respectively in the incubator, be equipped with the baffle between cultivation storehouse and the filling storehouse, it is equipped with the circulating pump to cultivate the box up end, the input of circulating pump is equipped with the drinking-water pipe, and the drinking-water pipe of institute extends right and is 90 degrees stretch into after buckling downwards again in the incubator, the output of circulating pump is equipped with circulating line. The utility model discloses can the circulation culture stem cell tissue fluid, the effectual activity that promotes stem cell tissue fluid, and this equipment easy operation, the filling is convenient.

Description

Stem cell culture perfusion device

Technical Field

The utility model relates to a stem cell culture technical field specifically is a stem cell culture perfusion device.

Background

Stem cells are a class of cells that have unlimited or immortal self-renewal capacity, are capable of producing at least one type of highly differentiated progeny cells, and have been subject to revision over the years and definition at different levels. At present, most biologists and physicians consider that stem cells are a type of cells which come from embryos, fetuses or adults and have the capacity of self-renewal and proliferation and differentiation without limitation under certain conditions, can generate daughter cells with completely the same phenotype and genotype as well as self, can also generate specialized cells forming tissues and organs of a body, and can also be differentiated into progenitor cells;

for example, application numbers are: the patent of CN215050386U comprises a shell, an inner container, a liquid inlet pipe and a liquid outlet pipe, wherein the inner container is arranged in an inner cavity of the shell, the liquid inlet pipe is connected to one side of the surface of the top of the shell in an embedded manner, the liquid outlet pipe is connected to the surface of the bottom of the shell in an embedded manner, a protection frame is arranged in the middle of the surface of the top of the shell, a servo motor is arranged in an inner cavity of the protection frame, a stirring paddle is arranged in the inner cavity of the inner container, an electric heating plate is arranged on the surface of the outer wall of the inner cavity of the inner container, heating liquid is filled between the shell and the inner container, a protection box is welded in the middle of the right side wall of the shell, a time control switch is arranged at the top of the surface of the protection box, a temperature adjusting button is arranged at the bottom of the surface of the protection box, and the bottom end of the liquid inlet pipe is connected with the inner container in a conduction manner;

although the design has the function of double protection of the stem cells, the stem cells can be stirred and heated conveniently, so that the activity of the stem cells can be improved, the post-perfusion treatment of the stem cells is facilitated, and the like;

however, the stem cell tissue fluid is directly stirred and contacted, the stem cell tissue fluid is damaged, and the device cannot be filled after the stem cell tissue fluid is cultured.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a stem cell culture perfusion device to solve the problems mentioned in the background art;

for realizing the above-mentioned purpose, the utility model provides a stem cell culture perfusion device, including cultivateing box and a plurality of filling subassembly, from last cultivation storehouse and the filling storehouse of down being equipped with respectively in the cultivation box, it is equipped with the baffle to cultivate between storehouse and the filling storehouse, it is equipped with the circulating pump to cultivate the box up end, the input of circulating pump is equipped with the drinking-water pipe, and the drinking-water pipe that institute extends right is 90 downward again and stretches into after buckling to in the cultivation box, the output of circulating pump is equipped with circulating line.

Preferably: circulating line is including installing the first pipe of circulating pump output, first union coupling has downwardly extending's second pipe, the second pipe runs through the baffle, the second pipe connection has the third pipe that extends right, the third pipe runs through cultivate the right wall of box, the third pipe connection has the fourth pipe that upwards extends, the fourth pipe connection has the fifth pipe that extends left, the fifth pipe runs through cultivate the right wall of box and stretch into cultivate in the storehouse.

Preferably: and a first electric valve is arranged on the outer wall of the fourth guide pipe.

Preferably: the filling assembly comprises a discharge pipe arranged on the outer wall of the third guide pipe, a second electric valve is arranged on the outer wall of the discharge pipe, and a spray head is arranged on the lower end face of the third guide pipe.

Preferably: the culture box is characterized in that a connecting seat is arranged on the upper end face of the culture box body, an inserting groove is formed in the connecting seat and communicated with the culture bin, a drainage tube is detachably mounted in the inserting groove, a liquid guide tube is arranged on the upper end face of the drainage tube, and a funnel is arranged on the upper end face of the liquid guide tube.

Preferably: the catheter is S-shaped.

Preferably: the culture bin is internally provided with a storage box, the upper end face of the storage box is provided with a plurality of placing grooves which are concave inwards, the placing grooves are internally and movably connected with test tubes, and the right end face of the storage box is provided with a pulling block.

Preferably, the following components: the left end face of the culture box body is provided with a controller, and the controller is electrically connected with the circulating pump, the first electric valve and the second electric valve through leads respectively.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model discloses a set up the circulating pump, set up drinking-water pipe and circulating line through input and output at the circulating pump, can make stem cell tissue fluid when cultivateing, can keep the activity of stem cell tissue fluid, through setting up the filling subassembly, can carry out filling work with the stem cell tissue fluid that has cultivateed.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is an enlarged view of A of the present invention;

fig. 3 is an enlarged view of B of the present invention.

In the figure: 1. a culture box body; 11. a culture bin; 12. filling a bin; 13. a partition plate; 14. a circulation pump; 141. a water pumping pipe; 142. a circulation pipe; 1421. a first conduit; 1422. a second conduit; 1423. a third conduit; 1424. a fourth conduit; 1425. a fifth conduit; 1426. a first electrically operated valve; 15. a connecting seat; 151. a slot; 16. a drainage tube; 161. a catheter; 162. a funnel; 17. a controller; 2. filling the assembly; 21. a discharge pipe; 22. a second electrically operated valve; 23. a spray head; 3. a storage box; 31. a placement groove; 32. a test tube; 33. and (5) pulling the block.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully with reference to the accompanying drawings, in which several embodiments of the present invention are shown, but the present invention can be implemented in different forms, and is not limited to the embodiments described in the text, but rather, these embodiments are provided to make the disclosure of the present invention more thorough and comprehensive.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may be present, and when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present, as the terms "vertical", "horizontal", "left", "right" and the like are used herein for descriptive purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in the specification of the present invention are for the purpose of describing particular embodiments only and are not intended to limit the present invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

The present invention will be described in further detail with reference to the accompanying drawings.

Example one

Please refer to fig. 1-3, the utility model provides a stem cell culture perfusion device, including cultivateing box 1 and a plurality of filling subassembly 2, cultivate in the box 1 from last down being equipped with respectively and cultivate storehouse 11 and filling storehouse 12, it is equipped with baffle 13 to cultivate between storehouse 11 and the filling storehouse 12, it is equipped with circulating pump 14 to cultivate 1 up end of box, circulating pump 14's input is equipped with drinking-water pipe 141, and drinking-water pipe 141 extends right and is 90 degrees after buckling downwards again and stretch into in the cultivation box 1, circulating pump 14's output is equipped with circulating line 142.

Please refer to fig. 1, the circulation pipeline 142 includes a first conduit 1421 installed at an output end of the circulation pump 14, the first conduit is connected to a second conduit 1422 extending downward, the second conduit 1422 penetrates the partition 13, the second conduit 1422 is connected to a third conduit 1423 extending rightward, the third conduit 1423 penetrates a right wall of the cultivation box 1, the third conduit 1423 is connected to a fourth conduit 1424 extending upward, the fourth conduit 1424 is connected to a fifth conduit 1425 extending leftward, the fifth conduit 1425 penetrates a right wall of the cultivation box 1 and extends into the cultivation bin 11, the circulation of the stem cell tissue fluid can be completed, an outer wall of the fourth conduit 1424 is provided with a first electric valve 1426, the first electric valve 1426 can be opened and closed, the fourth conduit 1424 can be opened and closed, the cultivation bin 11 is provided with a storage box 3, an upper end surface of the storage box 3 is provided with a plurality of inward placing grooves 31, a movable test tube 32 is connected to the placing groove 31, a movable storage box 3 is provided with a storage box 3, a left end surface of the storage box 3 can be connected to a control device, and a left end surface of the storage box 14 and a control device can be connected to the control device, the control device 17, and the control device can be connected to the control device.

Referring again to fig. 2, the filling assembly 2 includes a discharging pipe 21 installed on an outer wall of the third guide pipe 1423, a second electric valve 22 is installed on an outer wall of the discharging pipe 21, a spray head 23 is installed on a lower end surface of the third guide pipe 1423, and the second electric valve 22 can open and close the fourth guide pipe 1424.

Please refer to fig. 1 and fig. 3, the upper end surface of the culture box body 1 is provided with a connection seat 15, the connection seat 15 is provided with a slot 151, the slot 151 is communicated with the culture chamber 11, a drainage tube 16 is detachably installed in the slot 151, the upper end surface of the drainage tube 16 is provided with a catheter 161, the upper end surface of the catheter 161 is provided with a funnel 162, stem cell tissue fluid can be poured into the funnel 162, the catheter 161 can isolate impurities such as bacteria and dust during the culture process of the stem cell tissue fluid, and the catheter 161 is S-shaped, so that the design can isolate impurities such as bacteria and dust.

The embodiment of the utility model provides a concrete operation mode as follows:

when the device is used, firstly, the device is powered on, stem cell tissue fluid is poured into the funnel 162, and then is input into the culture bin 11 through the liquid guide tube 161 and the drainage tube 16, after sufficient stem cell tissue fluid is poured, the pouring operation is stopped, at the moment, the circulating pump 14 is started through the controller 17, the circulating pump 14 outputs the stem cell tissue fluid to the first conduit 1421 through the water pumping tube 141, and then sequentially passes through the second conduit 1422, the third conduit 1423, the fourth conduit 1424 and the fifth conduit 1425 to flow back into the culture bin 11, attention needs to be paid, at the moment, the first electric valve 1426 is in an open state, and the first electric valve 1426 is in a closed state, so that the circulating and backflow operation of the stem cell tissue fluid can be realized, the activity of the stem cell tissue fluid is ensured, when the stem cell tissue fluid is cultured to a required use degree, and when filling is required:

filling: placing the test tube 32 into the placing groove 31 of the storage box 3, installing the storage box 3 in the filling bin 12, and positioning the opening of the test tube 32 below the spray head 23, closing the first electric valve 1426 by the controller 17, opening the second electric valve 22, so that the stem cell tissue fluid can not perform circulation reflux work due to the closing of the first electric valve 1426, but can be filled in the test tube 32 through the lower end spray head 23 of the discharge tube 21;

after accomplishing first batch filling, cultivate the storehouse 11 this moment and have wantonly stored a large amount of stem cell tissue liquid, need close second electric valve 22 through controller 17, open first electric valve 1426, make stem cell tissue liquid continue to circulate work, grasp and draw piece 33 to take out receiver 3, take out the test tube 32 that the filling has stem cell tissue liquid, put into standing groove 31 with the test tube 32 that does not fill again and refer to the filling step and carry out the secondary filling.

The above description is taken as an example to describe the present invention with reference to the accompanying drawings, and it is obvious that the present invention is not limited by the above-mentioned manner, as long as the present invention is adopted, the method concept and the technical solution of the present invention are performed to perform this kind of insubstantial improvement, or the concept and the technical solution of the present invention are directly applied to other occasions without improvement, all within the protection scope of the present invention.

Claims

1. The utility model provides a stem cell culture perfusion device, includes culture box body (1) and a plurality of filling subassembly (2), its characterized in that: cultivate in box (1) from last down and be equipped with respectively and cultivate storehouse (11) and filling storehouse (12), it is equipped with baffle (13) to cultivate between storehouse (11) and the filling storehouse (12), it is equipped with circulating pump (14) to cultivate box (1) up end, the input of circulating pump (14) is equipped with drinking-water pipe (141), and drinking-water pipe (141) extend right again and be 90 after buckling stretch into downwards cultivate in box (1), the output of circulating pump (14) is equipped with circulating line (142).

2. A stem cell culture perfusion apparatus as claimed in claim 1, wherein: the circulating pipeline (142) comprises a first guide pipe (1421) installed at the output end of the circulating pump (14), the first guide pipe is connected with a second guide pipe (1422) extending downwards, the second guide pipe (1422) penetrates through the partition plate (13), the second guide pipe (1422) is connected with a third guide pipe (1423) extending rightwards, the third guide pipe (1423) penetrates through the right wall of the culture box body (1), the third guide pipe (1423) is connected with a fourth guide pipe (1424) extending upwards, the fourth guide pipe (1424) is connected with a fifth guide pipe (1425) extending leftwards, and the fifth guide pipe (1425) penetrates through the right wall of the culture box body (1) and extends into the culture bin (11).

3. A stem cell culture perfusion apparatus as defined in claim 2, further comprising: and a first electric valve (1426) is arranged on the outer wall of the fourth conduit (1424).

4. A stem cell culture perfusion apparatus as defined in claim 3, further comprising: filling subassembly (2) including install arrange material pipe (21) of third pipe (1423) outer wall, it is equipped with second electric valve (22) to arrange material pipe (21) outer wall, the lower terminal surface of third pipe (1423) is equipped with shower nozzle (23).

5. A stem cell culture perfusion apparatus as claimed in claim 4, wherein: cultivate box (1) up end and be equipped with connecting seat (15), connecting seat (15) are equipped with slot (151), slot (151) with cultivate storehouse (11) and be linked together, demountable installation has drainage tube (16) in slot (151), drainage tube (16) up end is equipped with catheter (161), catheter (161) up end is equipped with funnel (162).

6. A stem cell culture perfusion apparatus as claimed in claim 5, wherein: the catheter (161) is S-shaped.

7. A stem cell culture perfusion apparatus as claimed in claim 1, wherein: cultivate receiver (3) in storehouse (11), receiver (3) up end is equipped with standing groove (31) of a plurality of inside recesses, swing joint has test tube (32) in standing groove (31), receiver (3) right-hand member face is equipped with draws piece (33).

8. A stem cell culture perfusion apparatus as claimed in claim 1, wherein: the left end face of the culture box body (1) is provided with a controller (17), and the controller (17) is electrically connected with the circulating pump (14), the first electric valve (1426) and the second electric valve (22) through conducting wires.