CN218115433U - Culture flask strikes device - Google Patents
Culture flask strikes device Download PDFInfo
- Publication number
- CN218115433U CN218115433U CN202222079666.3U CN202222079666U CN218115433U CN 218115433 U CN218115433 U CN 218115433U CN 202222079666 U CN202222079666 U CN 202222079666U CN 218115433 U CN218115433 U CN 218115433U
- Authority
- CN
- China
- Prior art keywords
- bottle
- hammer
- fixed
- fixing
- lead screw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a device is beaten to blake bottle, including beating the subassembly and the fixed subassembly of blake bottle, hit the subassembly including beating the hammer and being used for the drive hit the straight line drive arrangement who beats hammer linear motion, the fixed subassembly of blake bottle includes PMKD, the last fixed establishment who is used for fixed blake bottle that is equipped with of PMKD. The utility model discloses a device is beaten to blake bottle, fixed establishment through on the PMKD fixes the blake bottle, recycles and hits the bottle end of beating the blake bottle of beating to hit, can make the cell that adheres to at the bottom of the blake bottle drop to improve the cell state.
Description
Technical Field
The utility model relates to a cell culture technical field particularly, for a device is beaten to blake bottle.
Background
In general, the cell culture is performed by using a culture flask, and when stem cells or other adherent cells are cultured by using the culture flask, the following conditions are met: when the cells are digested, the cells are almost digested, but part of the cells are attached to the bottom of the culture bottle, the cells attached to the bottom of the culture bottle are digested for a few more minutes under the condition that the cells do not fall off from the bottom of the culture bottle in time, although the cells can fall off from the bottom of the culture bottle, the state of the cells is worse than that of the cells falling off from the bottom wall of the culture bottle in time, and the method mainly comprises the following steps: 1. cell doubling time of later culture and passage is prolonged; 2. the generation number of passages is reduced; 3. the purity of the cells is reduced; 4. a deterioration in cell phenotype; 5. the uniformity of cell morphology (cell diameter, uniformity of cytoplasm) was poor. At present, the adopted mode is mainly that an experimenter lightly flicks a plurality of culture bottles with hands when cell digestion is finished, so that cells attached to the bottom surfaces of the culture bottles fall off and float in digestive juice to form round or round-like single cell balls, but the mode is easy to cause the phenomenon of incomplete cell separation.
Therefore, a need exists for a culture flask beating device.
Disclosure of Invention
In view of this, the utility model aims at providing a device is beaten to blake bottle can make attached cell in the blake bottle bottom surface drop to the culture solution, improves the state of cell.
In order to achieve the above purpose, the utility model provides a following technical scheme:
the utility model provides a device is hit to blake bottle, including hitting the fixed subassembly of beating subassembly and blake bottle, hit the subassembly including hitting the hammer and being used for the drive hit the linear drive device who beats hammer linear motion, the fixed subassembly of blake bottle includes PMKD, the last fixed establishment who is used for fixed blake bottle that is equipped with of PMKD.
Further, the linear driving device comprises a cylinder, a piston rod is arranged on the cylinder, and the striking hammer is arranged at the end of the piston rod.
Further, the linear driving device comprises a slider-crank mechanism, the slider-crank mechanism comprises a first sliding chute, a slider capable of reciprocating linear motion is arranged in the first sliding chute, and the striking hammer and the slider move synchronously.
Furthermore, one end of the sliding block extends out of the first sliding groove, and the striking hammer is arranged at the end part of the sliding block.
Furthermore, the fixing mechanism comprises two bottle bottom fixing check blocks and two bottle body fixing check blocks which are arranged on the fixing bottom plate, and a fixing groove for fixing the culture bottle is defined between the two bottle bottom fixing check blocks and the two bottle body fixing check blocks.
Further, a second sliding groove and a third sliding groove are formed in the fixed bottom plate, and sliding connection portions in sliding fit with the second sliding groove and the third sliding groove are respectively arranged on the bottle bottom fixed stop block and the bottle body fixed stop block.
Furthermore, the sliding connection part is provided with a thread section and further comprises a fastening nut which is used for being in thread fit with the thread section.
Furthermore, a first lead screw and a second lead screw are respectively arranged in the second sliding groove and the third sliding groove, and thread sleeves which are in threaded fit with the first lead screw and the second lead screw are respectively arranged on the bottle bottom fixed stop block and the bottle body fixed stop block.
Furthermore, a hammer face is arranged on the striking hammer, and the hammer face is made of elastic materials.
Furthermore, the striking hammer is made of elastic materials, or an elastic layer is sleeved outside the striking hammer.
The beneficial effects of the utility model reside in that:
the utility model discloses a device is beaten to blake bottle, fixed establishment through on the PMKD fixes the blake bottle, recycles and hits the bottle end of beating the blake bottle of beating to hit, can make the cell that adheres to at the bottom of the blake bottle drop to improve the cell state.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention will be realized and attained by the specification which follows.
Drawings
In order to make the purpose, technical scheme and beneficial effect of the utility model clearer, the utility model provides a following figure explains:
FIG. 1 is a front view of an embodiment 1 of the flask beating device of the present invention;
FIG. 2 is a plan view of an embodiment 1 of the culture flask beating device of the present invention;
FIG. 3 is a schematic view showing the connection between the slider-crank mechanism and the striking hammer in embodiment 2 of the present invention;
FIG. 4a is a first schematic view of the bottom surface of the fixed base plate;
FIG. 4b is a second schematic view of the bottom surface of the fixed base plate;
FIG. 5 is a schematic view of a bottle bottom fixing stopper;
fig. 6 is a schematic view of a bottle body fixing stopper.
Description of the reference numerals:
1-striking a hammer; 2-fixing the bottom plate; 3-a cylinder; 301-a piston rod; 4-a first runner; 5-a slide block; 6-fixing a stop block at the bottom of the bottle; 7-a bottle body fixing stop block; 8-a second chute; 9-a third chute; 10-a sliding connection; 11-a fastening nut; 12-a first lead screw; 13-a second lead screw; 14-thread insert; 15-culture flask.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not limited to the present invention.
Example 1
As shown in FIG. 1 and FIG. 2, there are a front view and a top view of the culture flask beating device of the present embodiment, respectively. The device is beaten to blake bottle of this embodiment, including beating subassembly and the fixed subassembly of blake bottle, beat the subassembly including beating hammer 1 and being used for the drive to beat the straight line drive arrangement of 1 linear motion of hammer, the fixed subassembly of blake bottle includes PMKD 2, is equipped with the fixed establishment who is used for fixed blake bottle on the PMKD 2.
Specifically, as shown in fig. 1 and 2, the linear drive device of the present embodiment includes a cylinder 3, a piston rod 301 is provided on the cylinder 3, and the striking hammer 1 is provided at an end of the piston rod 301. In this embodiment, the cylinder 3 is a magnetic piston cylinder. By controlling the extension and retraction of the piston rod 301 of the magnetic piston cylinder, the impact hammer 1 can apply a hammering action to the culture flask 15, so that the cells attached to the bottom wall of the culture flask fall off into the culture solution in the flask.
In this embodiment, the hammering interval time and hammering strength of the hammering hammer 1 can be controlled by using the control box, the hammering hammer 1 is usually hammed once in 1 second, and the strength is controlled within a range of 10-100N, so that the integrity of the cell membrane cannot be damaged by the strength.
As shown in fig. 1 and fig. 2, the fixing mechanism of the present embodiment includes two bottom fixing stoppers 6 and two body fixing stoppers 7 disposed on the fixing base plate 2, and a fixing groove for fixing the culture bottle is defined between the two bottom fixing stoppers 6 and the two body fixing stoppers 7, so that the culture bottle 15 can be stably fixed on the fixing base plate 2 during the striking process.
As shown in fig. 4a and 4b, in this embodiment, the size of the fixing groove formed between the two bottom fixing stoppers 6 and the two body fixing stoppers 7 can be adjusted in the following two ways, so as to be suitable for fixing culture bottles of different sizes.
As shown in fig. 4a, a first way: the fixed bottom plate 2 is provided with a second chute 8 and a third chute 9, the bottle bottom fixed stop 6 and the bottle body fixed stop 7 are respectively provided with a sliding connection part 10 in sliding fit with the second chute 8 and the third chute 9, the sliding connection part 10 is provided with a thread section, and the fixed bottom plate further comprises a fastening nut 11 in threaded fit with the thread section. Adopt the first mode, when using, can manually adjust the size of the fixed slot that forms between two fixed dog 6 at the bottom of the bottle and two fixed dog 7 of body, adjust the back of accomplishing, adopt fastening nut 11 with the fixed dog 6 at the bottom of the bottle and the fixed dog 7's of body position fix can.
As shown in fig. 4b, a second way: the second chute 8 and the third chute 9 are respectively provided with a first lead screw 12 and a second lead screw 13, the bottle bottom fixed stop 6 and the bottle body fixed stop 7 are respectively provided with a thread sleeve 14 in thread fit with the first lead screw 12 and the second lead screw 13, and the bottle further comprises a driving motor for respectively driving the first lead screw 12 and the second lead screw 13 to rotate. Adopt the second kind of mode, when using, can drive first lead screw 12 and second lead screw 13 through driving motor and rotate to the messenger lies in the 14 rectilinear movement of swivel nut on first lead screw 12 and the second lead screw 13 respectively, drives the fixed dog 6 in bottom of the bottle and the fixed dog 7 of body and removes again, and then adjusts the size of the fixed slot that forms between two fixed dogs 6 in bottom of the bottle and two fixed dogs 7 of body.
In order to prevent the striking hammer 1 from breaking the bottom of the culture bottle 15 during striking the culture bottle 15, the following measures can be adopted in the embodiment:
(1) The beating hammer 1 is provided with a hammer face which is made of elastic materials, specifically, the hammer face is flat (about 7cm long and about 2.5cm wide) in outline and used for increasing the contact area with the bottom of the culture bottle 15, and the hammer face is made of elastic materials and can further prevent the beating hammer 1 from breaking the bottom of the bottle;
(2) The striking hammer 1 is made of an elastic material;
(3) The outer sleeve of the striking hammer 1 is provided with an elastic layer.
Example 2
As shown in fig. 3, it is a schematic view of the beating assembly of the culture flask beating device of this embodiment. The difference from embodiment 1 is that the linear driving device of this embodiment adopts a slider-crank mechanism, the slider-crank mechanism includes a first chute 4, a slider 5 capable of reciprocating linear motion is arranged in the first chute 4, the striking hammer 1 and the slider 5 move synchronously, one end of the slider 5 extends out of the first chute 4, and the striking hammer 1 is arranged at the end of the slider 5. The cell attached to the bottom of the culture bottle 15 can be dropped into the culture solution by driving the hammer 1 to hammer the bottom of the culture bottle by the slider-crank mechanism.
Other settings of this embodiment are the same as those of embodiment 1, and are not described again.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutes or changes made by the technical personnel in the technical field on the basis of the utility model are all within the protection scope of the utility model. The protection scope of the present invention is subject to the claims.
Claims (10)
1. The utility model provides a device is beaten to blake bottle which characterized in that: including hitting subassembly and the fixed subassembly of blake bottle, hit the subassembly including hitting hammer (1) and being used for the drive hit hammer (1) linear motion's linear drive device, the fixed subassembly of blake bottle includes PMKD (2), be equipped with the fixed establishment who is used for fixed blake bottle on PMKD (2).
2. Culture bottle beating device according to claim 1, characterized in that: the linear driving device comprises a cylinder (3), a piston rod (301) is arranged on the cylinder (3), and the striking hammer (1) is arranged at the end of the piston rod (301).
3. Culture bottle beating device according to claim 1, characterized in that: the linear driving device comprises a slider-crank mechanism, the slider-crank mechanism comprises a first sliding chute (4), a slider (5) capable of performing reciprocating linear motion is arranged in the first sliding chute (4), and the striking hammer (1) and the slider (5) synchronously move.
4. A flask beating device according to claim 3, wherein: one end of the sliding block (5) extends out of the first sliding groove (4), and the striking hammer (1) is arranged at the end part of the sliding block (5).
5. Culture bottle beating device according to any one of claims 1-4, characterized in that: the fixing mechanism comprises two bottle bottom fixing check blocks (6) and two bottle body fixing check blocks (7) which are arranged on the fixing base plate (2), and a fixing groove for fixing the culture bottle is formed between the two bottle bottom fixing check blocks (6) and the two bottle body fixing check blocks (7).
6. A flask beating device according to claim 5, wherein: the bottle is characterized in that a second sliding groove (8) and a third sliding groove (9) are formed in the fixed bottom plate (2), and sliding connection parts (10) in sliding fit with the second sliding groove (8) and the third sliding groove (9) are respectively arranged on the bottle bottom fixed stop block (6) and the bottle body fixed stop block (7).
7. Culture bottle beating device according to claim 6, characterized in that: the sliding connection part (10) is provided with a thread section and further comprises a fastening nut (11) which is used for being in thread fit with the thread section.
8. Culture bottle beating device according to claim 6, characterized in that: the bottle comprises a bottle body, and is characterized in that a first lead screw (12) and a second lead screw (13) are respectively arranged in the second sliding groove (8) and the third sliding groove (9), thread sleeves (14) which are in threaded fit with the first lead screw (12) and the second lead screw (13) are respectively arranged on a bottle bottom fixed stop block (6) and a bottle body fixed stop block (7), and the bottle body further comprises a driving motor which respectively drives the first lead screw (12) and the second lead screw (13) to rotate.
9. Culture bottle beating device according to claim 1, characterized in that: the hammering hammer (1) is provided with a hammer face, and the hammer face is made of an elastic material.
10. Culture bottle beating device according to claim 1, characterized in that: the striking hammer (1) is made of elastic materials, or an elastic layer is sleeved outside the striking hammer (1).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202222079666.3U CN218115433U (en) | 2022-08-09 | 2022-08-09 | Culture flask strikes device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202222079666.3U CN218115433U (en) | 2022-08-09 | 2022-08-09 | Culture flask strikes device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN218115433U true CN218115433U (en) | 2022-12-23 |
Family
ID=84521868
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202222079666.3U Active CN218115433U (en) | 2022-08-09 | 2022-08-09 | Culture flask strikes device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN218115433U (en) |
-
2022
- 2022-08-09 CN CN202222079666.3U patent/CN218115433U/en active Active
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN2436182Y (en) | Improved golf club head | |
CN218115433U (en) | Culture flask strikes device | |
CN202087021U (en) | Rod head of golfball hitting rod for indoor golf | |
CN211020877U (en) | Hickory nut shell breaking machine | |
CN110558571B (en) | Walnut shell breaking and collecting device and using method | |
CN203379029U (en) | Walnut cracker | |
KR101944940B1 (en) | Putter head for golf | |
CN218710367U (en) | Fermentation tank capable of being internally broken | |
CN112192963A (en) | Advertisement inkjet printer capable of improving definition and freely adjusting height of nozzle | |
CN207157813U (en) | A kind of small household appliances storage equipment | |
CN217070512U (en) | Force eliminating structure for ejector push rod of press machine | |
US20060094536A1 (en) | Smooth roller | |
CN103830895B (en) | Push rod training aids | |
CN209613124U (en) | The tup of crusher | |
CN210544907U (en) | Essence stirred tank | |
CN210079733U (en) | Glass bottle body double-cavity crusher | |
CN208553058U (en) | A kind of service robot for athletic training | |
CN107952864B (en) | Powder metallurgy fanning strip answers punch die | |
CN202097723U (en) | Knocking device | |
CN214654894U (en) | Sampling structure special for bacteria isolated culture | |
CN220812411U (en) | Oxygen supply device for green alga culture | |
CN209682108U (en) | A kind of hammer structure | |
CN221062657U (en) | Automatic pressure relief device of reaction kettle | |
CN214830396U (en) | Semi-perfusion type culture dish for virus | |
CN214320914U (en) | Objective table for automatic quartz wafer sorting equipment |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |