CN218059040U - Aseptic isolator for cell product culture - Google Patents

Abstract

The utility model discloses an aseptic isolator of cell product culture, including the isolator cabin body, the isolator cabin body includes foundation frame, regulator cubicle, transmission cabin, operation cabin and carbon dioxide incubator, the surface of regulator cubicle is provided with the host computer, the surface of regulator cubicle and the below that is located the host computer are provided with gloves integrality leak detector. The utility model discloses, open the regulator cubicle earlier and switch on the isolator, then show various data signal (like hydraulic pressure, temperature etc.) on the screen through the host computer, at this moment under the generator control of transmission cabin inside, hydrogen peroxide steam in the hydrogen peroxide storage jar pours into the isolator cabin internally, all kinds of sensors in the isolator can monitor pressure and hydrogen peroxide steam concentration simultaneously, realize the sterilization operation, through setting up gloves integrality leak detector, can detect whether damaged the gloves that the staff wore, ensure the integrality of gloves, and the safety performance is improved.

Description

Aseptic isolator for cell product culture

Technical Field

The utility model relates to a cell product culture technology field specifically is aseptic isolator of cell product culture.

Background

Regenerative medicine is being widely developed today, and with the continuous and intensive research work in this field, the commercial manufacture of biological products is more and more emphasized, and from the viewpoint of the pharmaceutical product quality control regulations, the most critical challenge is aseptic handling due to special factors of cell handling. One possible solution to these challenges is isolation technology, commonly used in the pharmaceutical industry, and suggested by regulatory guidelines in this field, and thus the present invention provides a cell product culture sterile isolator to address the above-mentioned problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell product cultivates aseptic isolator to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

aseptic isolator is cultivateed to cell goods, including the isolator cabin body, the isolator cabin body includes basic frame, regulator cubicle, transmission cabin, operation cabin and carbon dioxide incubator, the surface of regulator cubicle is provided with the host computer, the surface of regulator cubicle and the below that is located the host computer are provided with gloves integrality leak detector, the inner chamber of operation cabin is provided with the cell observation microscope, the both sides of operation cabin inner chamber bottom are provided with discarded object passageway and cell centrifuge respectively, the bottom of operation cabin inner chamber is located cell centrifuge's rear end and is provided with on-line monitoring particle and planktonic bacteria sample thief respectively.

As a further scheme of the utility model, the left side on transmission cabin surface is provided with the transmission door through the hinge, the both sides on operation cabin surface all are provided with the operation door through the hydraulic stem.

As a still further aspect of the present invention, the transmission compartment and the operation compartment are located above the base frame, and the electrical cabinet is located on the left side of the base frame and the transmission compartment.

As the utility model discloses scheme further still, the inner chamber of foundation frame has set gradually hydrogen peroxide storage jar, collecting box and has placed the case from a left side to the right side, the transmission cabin is located the top of hydrogen peroxide storage jar.

As a further scheme of the utility model, the bottom of discarded object passageway extends to the inner chamber of collecting box, the top of placing the case is seted up flutedly, cytospin places inside the recess.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model discloses, open the regulator cubicle earlier and switch on the isolator, then show various data signal (like hydraulic pressure) on the screen through the host computer (temperature etc.), at this moment under the generator control of transmission cabin inside, hydrogen peroxide steam in the hydrogen peroxide storage jar pours into the isolator cabin internally, all kinds of sensors in the isolator can monitor pressure and hydrogen peroxide steam concentration simultaneously, realize sterilization operation, through setting up gloves integrality leak hunting appearance, can detect whether damaged to the gloves that the staff wore, ensure the integrality of gloves, the security performance is improved, and the carbon dioxide incubator can cultivate the cell, make things convenient for the staff to take experimental cells, and put into the operation cabin through the gloves circle and carry out centrifugal operation to the cell through cell centrifuge, the setting of on-line monitoring particle and plankton sampler simultaneously, can carry out real-time supervision with the plankton and the dust grain that the cell produced when centrifugal operation, treat after the centrifugation microscope, and finish observing the cell after the centrifugation through the cell observation, and the cell body that the record has been observed, the discarded object collecting box is put in to the accessible passageway centralized processing in the later stage, be convenient for.

Drawings

FIG. 1 is a schematic view of the present invention;

FIG. 2 is a side view of the structure of the present invention;

fig. 3 is a top view of the structure of the present invention.

In the figure: 1. an isolator pod; 2. a base frame; 3. an electrical cabinet; 4. a transfer chamber; 5. an operating cabin; 6. a carbon dioxide incubator; 7. an upper computer; 8. a glove integrity leak detector; 9. a cell observation microscope; 10. a waste channel; 11. a cell centrifuge; 12. monitoring particles on line; 13. a planktonic bacteria sampler; 14. a pass-through gate; 15. an operating door; 16. a hydrogen peroxide storage tank; 17. a collection box; 18. and (4) placing the box.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

The first embodiment is as follows:

referring to fig. 1 to 3, in the embodiment of the present invention, an aseptic isolator for cell product cultivation includes an isolator cabin 1, the isolator cabin 1 includes a base frame 2, an electrical cabinet 3, a transfer cabin 4, an operation cabin 5 and a carbon dioxide incubator 6, an upper computer 7 is disposed on the surface of the electrical cabinet 3, a glove integrity leak detector 8 is disposed on the surface of the electrical cabinet 3 and below the upper computer 7, a cell observation microscope 9 is disposed in the inner cavity of the operation cabin 5, a waste channel 10 and a cell centrifuge 11 are disposed on two sides of the bottom of the inner cavity of the operation cabin 5, an online monitoring particle 12 and a planktonic bacteria sampler 13 are disposed on the bottom of the inner cavity of the operation cabin 5 and at the rear end of the cell centrifuge 11, respectively, and through the arrangement of the base frame 2, the electrical cabinet 3, the transfer cabin 4, the operation cabin 5 and the carbon dioxide incubator 6, the cell product culture sterile isolator is constructed together, wherein the electrical cabinet 3 supplies power to the isolator, the transmission cabin 4 can inject hydrogen peroxide steam in the hydrogen peroxide storage tank 16 into the isolator cabin body 1 to realize sterilization operation, the operation cabin 5 realizes experiment operation on cells, the cooperation of the above structures is realized, when the system confirms that the hydrogen peroxide concentration in the isolator is reduced to 1ppm through the concentration sensor, the system can be switched from a ventilation mode to a production mode, the isolator can maintain the environment reaching ISO5 level in the cabin body in the mode, the sterile detection is carried out in the mode, the temperature and the humidity in the cabin body are continuously monitored, and when the temperature exceeds the specified limit, more fresh air is fed into the system to compensate the temperature. The pressure difference in the cabin body is also continuously monitored, and the leakage rate is not more than 0.5 percent of the volume of the cabin body per hour when the pressure is 400 Pa. When the pressure difference exceeds a specified limit or the glove is broken, the frequency of the air supply fan is increased to maintain the pressure difference.

Specifically, the left side of the surface of the transfer chamber 4 is provided with a transfer door 14 through a hinge, and both sides of the surface of the operation chamber 5 are provided with operation doors 15 through hydraulic rods.

Specifically, the transfer chamber 4 and the operation chamber 5 are located above the base frame 2, and the electrical cabinet 3 is located on the left side of the base frame 2 and the transfer chamber 4.

Specifically, the inner cavity of the base frame 2 is provided with a hydrogen peroxide storage tank 16, a collection box 17 and a placement box 18 in sequence from left to right, and the transfer chamber 4 is located above the hydrogen peroxide storage tank 16.

Specifically, the bottom of the waste channel 10 extends to the inner cavity of the collection box 17, the top of the placing box 18 is provided with a groove, and the cytospin 11 is placed in the groove.

The principle of the embodiment is as follows:

when the device is used, the electrical cabinet 3 is firstly opened to electrify the isolator, then various data signals (such as hydraulic pressure, temperature and the like) are displayed on a screen of the upper computer 7, at the moment, under the control of a generator inside the transmission cabin 4, hydrogen peroxide steam in the hydrogen peroxide storage tank 16 is injected into the isolator cabin body 1, meanwhile, various sensors in the isolator can monitor pressure and hydrogen peroxide steam concentration, sterilization operation is realized, through the arrangement of the glove integrity leak detector 8, whether gloves worn by workers are damaged can be detected, the integrity of the gloves is ensured, safety performance is improved, the carbon dioxide incubator 6 can culture the cells, the workers can conveniently take experimental cells, and put the experimental cells into the operation cabin 5 through glove rings and carry out centrifugal operation on the cells through the cell centrifuge 11, meanwhile, the arrangement of the particles 12 and the planktonic bacteria sampler 13 is monitored on line, planktonic bacteria and dust particles generated during centrifugal operation of the cells can be monitored in real time, after centrifugation is finished, the cells after centrifugation are observed through the cell observation microscope 9, the cells which are recorded are thrown into the cell body, the waste channel 10 can be conveniently treated in a later period, and the collection box is conveniently treated in a later period.

The isolator system is designed with a special PLC control system and a human-computer interface to control air treatment and air supply, system sterilization, system dehumidification, program process operation and monitoring, when VHP confirms that the concentration of hydrogen peroxide in the isolator is reduced to 1ppm through a concentration sensor, the system can be switched from a ventilation mode to a production mode, and the isolator can maintain an ISO 5-grade environment in a cabin body in the mode and can simultaneously meet the operation of two persons.

The standard parts used in the present application document can be purchased from the market, and can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts the conventional means of mature bolt, rivet, welding and the like in the prior art, the machines, parts and equipment adopt the conventional models in the prior art, the control mode is automatically controlled by a controller, the control circuit of the controller can be realized by simple programming of technicians in the field, the control circuit belongs to the common knowledge in the field, and the present application document is mainly used for protecting mechanical devices, so the control mode and the circuit connection are not explained in detail in the present application.

Above, only be the embodiment of the preferred of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the design of the present invention, equivalent replacement or change should be included in the protection scope of the present invention.

Claims (5)

1. Cell goods cultivates aseptic isolator, including the isolator cabin body (1), its characterized in that, the isolator cabin body (1) is including basic frame (2), regulator cubicle (3), transmission cabin (4), operation cabin (5) and carbon dioxide incubator (6), the surface of regulator cubicle (3) is provided with host computer (7), the below that the surface of regulator cubicle (3) just is located host computer (7) is provided with gloves integrality leak detector (8), the inner chamber of operation cabin (5) is provided with cell observation microscope (9), the both sides of operation cabin (5) inner chamber bottom are provided with discarded object passageway (10) and cell centrifuge (11) respectively, the rear end that the bottom of operation cabin (5) inner chamber is located cell centrifuge (11) is provided with on-line monitoring particle (12) and planktonic bacteria sample thief (13) respectively.

2. The cell product culture sterile isolator according to claim 1, wherein the left side of the surface of the transfer chamber (4) is provided with a transfer door (14) through a hinge, and the two sides of the surface of the operation chamber (5) are provided with operation doors (15) through hydraulic rods.

3. The cell product culture sterile isolator according to claim 1, wherein the transfer compartment (4) and the operation compartment (5) are located above the base frame (2), and the electrical cabinet (3) is located on the left side of the base frame (2) and the transfer compartment (4).

4. The cell product culture sterile isolator according to claim 1, wherein the inner cavity of the base frame (2) is provided with a hydrogen peroxide storage tank (16), a collection box (17) and a placement box (18) in sequence from left to right, and the transfer chamber (4) is positioned above the hydrogen peroxide storage tank (16).

5. The cell product culture sterile isolator according to claim 4, wherein the bottom of the waste channel (10) extends to the inner cavity of the collection box (17), the top of the placing box (18) is provided with a groove, and the cell centrifuge (11) is placed in the groove.

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