CN217997206U - Electromagnetic equipment for removing bacteria from cell solution - Google Patents
Electromagnetic equipment for removing bacteria from cell solution Download PDFInfo
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- CN217997206U CN217997206U CN202221771371.6U CN202221771371U CN217997206U CN 217997206 U CN217997206 U CN 217997206U CN 202221771371 U CN202221771371 U CN 202221771371U CN 217997206 U CN217997206 U CN 217997206U
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Abstract
The utility model provides an electromagnetic device for removing bacteria from cell solution, which comprises a cylindrical screen mesh arranged in a buffer solution container; the buffer solution container is arranged at the middle shaft part of the electromagnetic induction coil; a liquid inlet for injecting a buffer liquid is formed in the top of the cylindrical screen; when the electromagnetic equipment works in a sterilization working condition, the buffer solution injected from the liquid inlet is a solution containing cells, the cell membrane surfaces of the cells carry charges, and the magnetic field of the electromagnetic induction coil applies lateral electromagnetic force to the cells in the buffer solution containing the cells, so that the cells move towards the screen and are hung on the screen by the electromagnetic force; the utility model can separate the cells from the bacteria through electromagnetic force, thereby saving the cells which fail to be cultured due to the pollution of the bacteria and reducing the economic loss.
Description
Technical Field
The utility model belongs to the technical field of the cell culture technique and specifically relates to an electromagnetic equipment of bacterium is removed to cell solution.
Background
In the cell culture, if bacterial contamination occurs, the culture will fail, and it is a research direction to separate the cells from the bacteria in this case, thereby saving the cells which have failed in culture due to the contamination of the bacteria.
Disclosure of Invention
The utility model provides a cell solution removes electromagnetic equipment of bacterium can separate cell and bacterium through the electromagnetic force to save and lead to cultivateing the cell of failure because of polluting the bacterium, reduce economic loss.
The utility model adopts the following technical scheme.
An electromagnetic apparatus for the removal of bacteria from a cell solution, said electromagnetic apparatus comprising a cylindrical screen placed in a buffer container; the buffer solution container is arranged at the middle shaft part of the electromagnetic induction coil; a liquid inlet for injecting a buffer liquid is formed in the top of the cylindrical screen; when the electromagnetic equipment works in a sterilization working condition, the buffer solution injected from the liquid inlet is a solution containing cells, the cell membrane surfaces of the cells carry charges, and the magnetic field of the electromagnetic induction coil applies lateral electromagnetic force to the cells in the buffer solution containing the cells, so that the cells move towards the screen and are hung on the screen by the electromagnetic force.
The electromagnetic equipment also comprises a motor, wherein the motor drives the cylindrical screen through a gear and a crawler belt to rotate at a low speed in the buffer solution container; the electromagnetic induction coil is powered by external equipment through a transformer.
And a liquid outlet communicated with an external container is formed at the bottom of the cylindrical screen.
The buffer containing the cells was injected into the inlet port by a peristaltic pump.
When the electromagnetic equipment works in the degerming working condition, the flow speed of the buffer solution injected into the liquid inlet by the peristaltic pump is the low flow speed matched with the liquid quantity of the liquid outlet, so that the liquid quantity in the buffer solution container is stable.
When the electromagnetic equipment works under the cell collection working condition, the external container is a sterile container, the peristaltic pump injects a buffer solution without bacteria into the liquid inlet at a high flow rate, the electromagnetic induction coil is powered off, and the cells mounted on the screen mesh are flushed into the sterile container by the buffer solution.
The screen is a stainless steel screen with 10um screen hole size; the cell size in the buffer is above 10 um; the size range of the bacteria in the buffer solution is 0.5-5um; when the buffer solution flows through the mesh, the cells are intercepted by the mesh and the bacteria pass through the mesh.
The buffer was PH = 7.2.
The buffer container is filled with phosphate buffer.
The utility model can separate the cells from the bacteria through electromagnetic force, thereby saving the cells which fail to be cultured due to the pollution of the bacteria and reducing the economic loss.
Drawings
The invention will be described in further detail with reference to the following drawings and detailed description:
FIG. 1 is a schematic diagram of the present invention;
in the figure: 1-an electromagnetic induction coil; 2-a liquid inlet; 3-a buffer container; 4-cylindrical screen mesh; 5-a transformer; 6-outer container; 7-liquid outlet.
Detailed Description
As shown in FIG. 1, an electromagnetic device for removing bacteria from cell solution is characterized in that: the electromagnetic device comprises a cylindrical screen 4 placed in a buffer container 3; the buffer solution container is arranged at the middle shaft part of the electromagnetic induction coil 1; a liquid inlet 2 for injecting a buffer solution is formed in the top of the cylindrical screen; when the electromagnetic equipment works in a sterilization working condition, the buffer solution injected from the liquid inlet is a solution containing cells, the cell membrane surface of the cells carries charges, and the magnetic field of the electromagnetic induction coil applies lateral electromagnetic force to the cells in the buffer solution containing the cells, so that the cells move towards the screen and are hung on the screen by the electromagnetic force.
The electromagnetic equipment also comprises a motor, wherein the motor drives the cylindrical screen through a gear and a crawler belt to rotate at a low speed in the buffer solution container; the electromagnetic coil is powered by an external device via a transformer 5.
The bottom of the cylindrical screen is provided with a liquid outlet 7 communicated with an external container 6.
The buffer containing the cells was injected into the inlet port by a peristaltic pump.
When the electromagnetic equipment works in the degerming working condition, the flow velocity of the buffer solution injected into the liquid inlet by the peristaltic pump is the low flow velocity matched with the liquid quantity of the liquid outlet, so that the liquid quantity in the buffer solution container is stable.
When the electromagnetic equipment works under the cell collection working condition, the external container is a sterile container, the peristaltic pump injects a buffer solution without bacteria into the liquid inlet at a high flow rate, the electromagnetic induction coil is powered off, and the cells mounted on the screen mesh are flushed into the sterile container by the buffer solution.
The screen is a stainless steel screen with 10um screen hole size; the cell size in the buffer is above 10 um; the size range of the bacteria in the buffer solution is 0.5-5um; when the buffer solution flows through the mesh, the cells are intercepted by the mesh and the bacteria pass through the mesh.
The buffer was PH = 7.2.
The buffer container is filled with phosphate buffer.
Example (b):
this example uses the present device by the following steps;
(1) Filling the buffer liquid bin with phosphate buffer liquid;
(2) After the cells polluted by bacteria are centrifugally collected, 1mL of phosphate buffer solution is used for heavy suspension and injection into a liquid inlet;
(3) Continuously inputting a phosphate buffer solution from a liquid inlet by using a peristaltic pump at a flow rate of less than 20, adjusting the flow of the liquid outlet to maintain the volume of the liquid in a chamber, and hanging cells in the buffer solution on a screen under the action of electromagnetic force, so that bacteria cannot stay on the screen, thereby separating the cells from the bacteria;
(4) After 30 minutes, the electromagnetic field was stopped, bacteria-free buffer was introduced, the flow rate was increased to 100, the cells on the screen were washed off, and the external vessel was replaced with a sterile vessel to collect the separated cells.
Claims (9)
1. An electromagnetic device for removing bacteria from a cell solution, characterized in that: the electromagnetic apparatus comprises a cylindrical screen placed in a buffer container; the buffer solution container is arranged at the middle shaft part of the electromagnetic induction coil; a liquid inlet for injecting a buffer solution is formed in the top of the cylindrical screen; when the electromagnetic equipment works in a sterilization working condition, the buffer solution injected from the liquid inlet is a solution containing cells, the cell membrane surface of the cells carries charges, and the magnetic field of the electromagnetic induction coil applies lateral electromagnetic force to the cells in the buffer solution containing the cells, so that the cells move towards the screen and are hung on the screen by the electromagnetic force.
2. The electromagnetic apparatus for removing bacteria from a cell solution according to claim 1, wherein: the electromagnetic equipment also comprises a motor, wherein the motor drives the cylindrical screen through a gear and a crawler belt to rotate at a low speed in the buffer solution container; the electromagnetic induction coil is powered by external equipment through a transformer.
3. The electromagnetic apparatus for removing bacteria from a cell solution as claimed in claim 1, wherein: and a liquid outlet communicated with an external container is formed at the bottom of the cylindrical screen.
4. The electromagnetic apparatus for removing bacteria from a cell solution as claimed in claim 3, wherein: the buffer containing the cells was injected into the inlet port by a peristaltic pump.
5. The electromagnetic apparatus for removing bacteria from a cell solution according to claim 3, wherein: when the electromagnetic equipment works in the degerming working condition, the flow velocity of the buffer solution injected into the liquid inlet by the peristaltic pump is the low flow velocity matched with the liquid quantity of the liquid outlet, so that the liquid quantity in the buffer solution container is stable.
6. The electromagnetic apparatus for removing bacteria from a cell solution according to claim 3, wherein: when the electromagnetic equipment works under the cell collection working condition, the external container is a sterile container, the peristaltic pump injects a buffer solution without bacteria into the liquid inlet at a high flow rate, the electromagnetic induction coil is powered off, and the cells mounted on the screen mesh are flushed into the sterile container by the buffer solution.
7. The electromagnetic apparatus for removing bacteria from a cell solution as claimed in claim 1, wherein: the screen is a stainless steel screen with the size of 10um screen holes; the cell size in the buffer is above 10 um; the size range of the bacteria in the buffer solution is 0.5-5um; as the buffer fluid flows through the mesh, the cells are intercepted by the mesh and the bacteria pass through the mesh.
8. The electromagnetic apparatus for removing bacteria from a cell solution according to claim 1, wherein: the buffer was PH = 7.2.
9. The electromagnetic apparatus for removing bacteria from a cell solution according to claim 1, wherein: the buffer container is filled with phosphate buffer.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2022215226640 | 2022-06-18 | ||
| CN202221522664 | 2022-06-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217997206U true CN217997206U (en) | 2022-12-09 |
Family
ID=84314093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221771371.6U Active CN217997206U (en) | 2022-06-18 | 2022-07-11 | Electromagnetic equipment for removing bacteria from cell solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217997206U (en) |
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2022
- 2022-07-11 CN CN202221771371.6U patent/CN217997206U/en active Active
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