CN217981369U - Two-dimensional liquid chromatography separation system - Google Patents
Two-dimensional liquid chromatography separation system Download PDFInfo
- Publication number
- CN217981369U CN217981369U CN202221904045.8U CN202221904045U CN217981369U CN 217981369 U CN217981369 U CN 217981369U CN 202221904045 U CN202221904045 U CN 202221904045U CN 217981369 U CN217981369 U CN 217981369U
- Authority
- CN
- China
- Prior art keywords
- unit
- separation
- way valve
- liquid chromatography
- pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses a two-dimensional liquid chromatography separation system, which comprises a sample introduction unit, a gradient elution unit, a separation unit, a detector, a capture unit and a collection unit; the sample introduction unit is connected with the inlet of the separation unit; the gradient elution unit is respectively connected with inlets of the separation unit and the capture unit through a first two-position four-way valve, the collection unit is respectively connected with outlets of the separation unit and the capture unit through a second two-position four-way valve, and a detector is arranged between the separation unit and the second two-position four-way valve; and the system is switched from a one-dimensional separation mode to a two-dimensional separation mode by switching the first two-position four-way valve and the second two-position four-way valve. The two-dimensional liquid chromatography separation system is suitable for separating complex components with less content, and the separated product has high purity, high recovery rate, high automation degree, low manpower and material resource consumption and high separation efficiency.
Description
Technical Field
The utility model belongs to the technical field of liquid chromatography separation, concretely relates to two-dimentional liquid chromatography piece-rate system.
Background
The waste green tobacco leaves are rich in high-quality protein, starch, grease, polyphenol, chlorophyll, carotenoid and other bioactive components, so the economic benefit of development and utilization is considerable.
At present, components of waste green tobacco leaves are mainly recovered by a crystallization method and an extraction method in the market at home, and the tobacco leaves obtained by the crystallization method and the extraction method have the advantages of single variety of effective components, low purity, low automation degree, large consumption of manpower and material resources and low separation efficiency.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned prior art's defect, the utility model provides a two-dimensional liquid chromatography separation system is applicable to the separation of the less complicated component of content, and the product purity after the separation is high, and the rate of recovery is high.
The utility model provides a technical scheme that its technical problem adopted is:
a two-dimensional liquid chromatography separation system comprises a sample introduction unit, a gradient elution unit, a separation unit, a detector, a trapping unit and a collection unit; the sample introduction unit is connected with the inlet of the separation unit; the gradient elution unit is respectively connected with inlets of the separation unit and the capture unit through a first two-position four-way valve, the collection unit is respectively connected with outlets of the separation unit and the capture unit through a second two-position four-way valve, and a detector is arranged between the separation unit and the second two-position four-way valve; and the system is switched from a one-dimensional separation mode to a two-dimensional separation mode by switching the first two-position four-way valve and the second two-position four-way valve.
And the sample injection unit, the gradient elution unit, the separation unit, the trapping unit and the collection unit are all in communication connection with the signal output end of the controller.
The device further comprises a dilution unit, wherein the dilution unit comprises a dilution pump and a second mixer, the dilution pump and a second two-position four-way valve are both connected with the inlet of the second mixer, and the outlet of the second mixer is connected with a first two-position four-way valve; the dilution pump is in communication connection with the signal output end of the controller.
Further, the sampling unit includes sampling pump and three-way valve, the sampling pump passes through the three-way valve and is connected with the separation element entry, first two cross valves and three-way valve connection, sampling pump, three-way valve and first two cross valves all with controller signal output part communication connection.
Furthermore, the separation unit is formed by connecting a plurality of separation columns in parallel through a third two-position four-way valve, and one of the separation columns is conducted at the same separation moment; the trapping unit is formed by connecting a plurality of trapping columns in parallel through a fourth two-position four-way valve, and one of the trapping columns is conducted at the same separation moment; and the third two-position four-way valve and the fourth two-position four-way valve are both in communication connection with the signal output end of the controller.
Furthermore, the collecting unit comprises a plurality of collecting valves and a waste liquid valve, the collecting valves and the waste liquid valve are connected in parallel, and one of the collecting valves is communicated at the same separation moment; the collection valve is in communication connection with the signal output end of the controller.
Further, the gradient elution unit comprises a first gradient pump, a second gradient pump and a first mixer, wherein the first gradient pump and the second gradient pump are both connected with the inlet of the first mixer, and the outlet of the first mixer is connected with a first two-position four-way valve; and the first gradient pump and the second gradient pump are both in communication connection with the signal output end of the controller.
Furthermore, the first two-position four-way valve, the second two-position four-way valve, the third two-position four-way valve, the fourth two-position four-way valve and the three-way valve are all electromagnetic valves, and the collecting valve is an air filling valve.
Further, the packing in the separation column comprises one of reversed phase packing, ion exchange packing, molecular sieve packing and affinity chromatography packing.
Further, the packing in the trap column includes one of reversed phase packing and polymer hydrophobic resin packing.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses a two-dimensional liquid chromatography separation system, including the appearance unit of advancing, gradient elution unit, the separation element, the detector, entrapment unit and collection unit, appearance unit and separation element entry linkage advance, gradient elution unit is connected with the entry linkage of separation element and entrapment unit respectively through first two cross valves, the collection unit is connected with the exit linkage of separation element and entrapment unit respectively through second two cross valves, be equipped with the detector between separation element and the second two cross valves, through switching over first two cross valves and second two cross valves, be used for making the system switch over from one-dimensional separation mode to two-dimensional separation mode; in the one-dimensional separation mode, the gradient elution unit is communicated with the inlet of the separation unit through a first two-position four-way valve, the outlet of the separation unit is communicated with the inlet of the capture unit through a second two-position four-way valve and a first two-position four-way valve in sequence, and the outlet of the capture unit is communicated with the collection unit through a second two-position four-way valve; because each first-stage separation component captured by the capturing unit is still a mixture, each first-stage separation component needs to be respectively subjected to two-dimensional separation, under the two-dimensional separation mode, the gradient elution unit is communicated with the inlet of the capturing unit through the first two-position four-way valve, the outlet of the capturing unit is communicated with the inlet of the separation unit through the second two-position four-way valve and the first two-position four-way valve in sequence, the outlet of the separation unit is communicated with the collection unit through the second two-position four-way valve, so that the gradient elution unit elutes a certain first-stage separation component captured by the capturing unit through gradient eluent, the first-stage separation component is eluted and is injected into the separation unit along with the gradient eluent, and each second-stage separation component in the first-stage separation component is separated by the separation unit in sequence and is collected by the collection unit in sequence; therefore, the two-dimensional liquid chromatography separation system can be used for two-dimensional separation of complex components with less content, and the separated product has high purity and high recovery rate.
The two-dimensional liquid chromatography separation system also comprises a controller, the detector is in communication connection with the signal input end of the controller, and the sample introduction unit, the gradient elution unit, the separation unit, the capture unit and the collection unit are all in communication connection with the signal output end of the controller; under a one-dimensional separation mode, the detector detects primary separation components in effluent liquid of the separation unit in real time, and feeds a detection result back to the controller, and the controller controls the trapping unit according to set parameters to trap specific primary separation components; under a two-dimensional separation mode, the detector detects secondary separation components in effluent liquid of the separation unit in real time, and feeds back detection results to the controller, and the controller controls the collection unit according to set parameters so as to collect specific secondary separation components; therefore, the two-dimensional liquid chromatography separation system is high in automation degree, low in manpower and material resource consumption and high in separation efficiency due to the arrangement of the controller.
In the utility model, the two-dimensional liquid chromatography separation system also comprises a dilution unit, the dilution unit comprises a dilution pump and a second mixer, the dilution pump and a second two-position four-way valve are both connected with the inlet of the second mixer, and the outlet of the second mixer is connected with a first two-position four-way valve; thus, in the one-dimensional separation mode, the first-stage separation component in the effluent of the separation unit can be better captured by the capture unit after being diluted, and in the two-dimensional separation mode, the first-stage separation component in the effluent of the capture unit can be better fed into the separation unit after being diluted.
In the utility model, the separation unit is formed by connecting a plurality of separation columns in parallel through a third two-position four-way valve, one of the separation columns is switched on at the same separation moment, the collection unit is formed by connecting a plurality of collection columns in parallel through a fourth two-position four-way valve, one of the collection columns is switched on at the same separation moment, the collection unit comprises a plurality of collection valves and a waste liquid valve, the collection valves are connected in parallel with the waste liquid valve, and one of the collection valves is switched on at the same separation moment; the arrangement of the plurality of separation columns is convenient for one-dimensional and two-dimensional separation of samples, the arrangement of the plurality of trapping columns is convenient for trapping each one-level separation component which is eluted and separated successively, and the arrangement of the plurality of collecting valves is convenient for collecting a plurality of two-level separation components which are separated successively.
Drawings
FIG. 1 is a schematic structural diagram of a two-dimensional liquid chromatography separation system of the present invention during isocratic operation before sample introduction;
FIG. 2 is a schematic structural diagram of the two-dimensional liquid chromatography separation system in a sample injection mode;
FIG. 3 is a schematic structural diagram of the two-dimensional liquid chromatography separation system in a one-dimensional separation mode;
fig. 4 is a schematic structural diagram of the two-dimensional liquid chromatography separation system in a two-dimensional separation mode.
The reference numbers in the figures illustrate that: 1. the system comprises a three-way valve, a first two-position four-way valve, a second two-position four-way valve, a third two-position four-way valve, a 4, a third two-position four-way valve, a 5, a fourth two-position four-way valve, a 6, a collecting valve, a 7 and a waste liquid valve.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to the attached drawings. These embodiments are only for illustrating the present invention and are not to be construed as limiting the present invention.
In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the system or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
As shown in fig. 1-4, a two-dimensional liquid chromatography separation system comprises a sample introduction unit, a gradient elution unit, a separation unit, a detector, a capture unit, a collection unit and a connecting pipeline; the sample introduction unit is connected with the inlet of the separation unit; the gradient elution unit is respectively connected with inlets of the separation unit and the capture unit through a first two-position four-way valve 2, the collection unit is respectively connected with outlets of the separation unit and the capture unit through a second two-position four-way valve 3, and a detector is arranged between the separation unit and the second two-position four-way valve 3; and the system is switched from a one-dimensional separation mode to a two-dimensional separation mode by switching the first two-position four-way valve 2 and the second two-position four-way valve 3.
A sample to be separated is injected into the separation unit through the sample injection unit, as shown in fig. 3, in a one-dimensional separation mode, the gradient elution unit is communicated with the inlet of the separation unit through the first two-position four-way valve 2, the outlet of the separation unit is communicated with the inlet of the capture unit through the second two-position four-way valve 3 and the first two-position four-way valve 2 in sequence, and the outlet of the capture unit is communicated with the collection unit through the second two-position four-way valve 3, so that the gradient elution unit performs gradient elution separation on the sample to be separated injected into the separation unit through gradient eluent, and each one-stage separation component in the sample to be separated is eluted and separated in sequence and captured by the capture unit in sequence, so as to realize the one-dimensional separation of each one-stage separation component in the sample to be separated, and the elution waste liquid flowing out of the capture unit is collected by the collection unit; as each primary separation component captured by the capturing unit is still a mixture, each primary separation component needs to be separated in two dimensions, as shown in fig. 4, in the two-dimensional separation mode, the gradient elution unit is communicated with the inlet of the capturing unit through the first two-position four-way valve 2, the outlet of the capturing unit is communicated with the inlet of the separation unit through the second two-position four-way valve 3 and the first two-position four-way valve 2 in sequence, the outlet of the separation unit is communicated with the collection unit through the second two-position four-way valve 3, so that the gradient elution unit elutes a certain primary separation component captured by the capturing unit through gradient eluent, the primary separation component is eluted and injected into the separation unit along with the gradient eluent, and each secondary separation component in the primary separation component is separated by the separation unit in sequence and collected by the collection unit in sequence; therefore, the two-dimensional liquid chromatography separation system can be used for two-dimensional separation of complex components with less content, and the separated product has high purity and high recovery rate.
In one embodiment, the two-dimensional liquid chromatography separation system further comprises a controller, the detector is in communication with a signal input end of the controller, and the sample introduction unit, the gradient elution unit, the separation unit, the capture unit and the collection unit are in communication with a signal output end of the controller. As shown in fig. 3, in the one-dimensional separation mode, the detector detects the primary separated component in the effluent of the separation unit in real time and feeds the detection result back to the controller, and the controller controls the trapping unit according to the set parameters to trap the specific primary separated component; as shown in fig. 4, in the two-dimensional separation mode, the detector detects the secondary separated components in the effluent of the separation unit in real time and feeds the detection result back to the controller, and the controller controls the collection unit according to the set parameters to collect the specific secondary separated components; therefore, the two-dimensional liquid chromatography separation system is high in automation degree, low in manpower and material resource consumption and high in separation efficiency due to the arrangement of the controller.
In one embodiment, the two-dimensional liquid chromatography separation system further comprises a dilution unit, wherein the dilution unit comprises a dilution pump and a second mixer, the dilution pump and a second two-position four-way valve 3 are both connected with the inlet of the second mixer, the outlet of the second mixer is connected with a first two-position four-way valve 2, and the second mixer is a static mixer or a dynamic mixer; the dilution pump is in communication connection with the signal output end of the controller. The second mixer is used for mixing the diluent and the effluent liquid which are pumped by the diluent pump, under a one-dimensional separation mode, the first-stage separation component in the effluent liquid of the separation unit can be better captured by the capture unit after being diluted, and under a two-dimensional separation mode, the first-stage separation component in the effluent liquid of the capture unit can be better fed into the separation unit after being diluted.
In one embodiment, the sample injection unit comprises a sample injection pump and a three-way valve 1, the sample injection pump is connected with the inlet of the separation unit through the three-way valve 1, a first two-position four-way valve 2 is connected with the three-way valve 1, and the sample injection pump, the three-way valve 1 and the first two-position four-way valve 2 are all in communication connection with the signal output end of the controller. As shown in fig. 2, in the sample injection mode, the controller controls the three-way valve 1 to communicate the three-way valve 1 with the sample injection pump, and controls the sample injection pump to inject the sample to be separated into the separation unit, as shown in fig. 3, in the one-dimensional separation mode, the controller controls the three-way valve 1 to communicate the three-way valve 1 with the first two-position four-way valve 2, so that the gradient elution unit is communicated with the inlet of the separation unit.
In one embodiment, the separation unit is formed by connecting a plurality of separation columns in parallel through a third two-position four-way valve 4, one separation column is conducted at the same separation moment, the trapping unit is formed by connecting a plurality of trapping columns in parallel through a fourth two-position four-way valve 5, one trapping column is conducted at the same separation moment, the collection unit comprises a plurality of collection valves 6 and a waste liquid valve 7, the collection valves 6 and the waste liquid valve 7 are connected in parallel, one collection valve 6 is conducted at the same separation moment, and the third two-position four-way valve 4, the fourth two-position four-way valve 5 and the collection valve 6 are all in communication connection with the signal output end of the controller. The arrangement of a plurality of separation columns is convenient for one-dimensional and two-dimensional separation of samples, the arrangement of a plurality of trapping columns is convenient for respectively trapping each first-stage separation component which is eluted and separated successively, and the arrangement of a plurality of collecting valves 6 is convenient for respectively collecting a plurality of second-stage separation components which are separated successively; and in the one-dimensional separation mode, the detector detects the primary separation component in the effluent liquid of the corresponding separation column in real time and feeds the detection result back to the controller, the controller controls the corresponding fourth two-position four-way valve 5 according to the set parameters to trap the specific primary separation component through the corresponding trapping column, as shown in figure 3, in the two-dimensional separation mode, the detector detects the secondary separation component in the effluent liquid of the corresponding separation column in real time and feeds the detection result back to the controller, and the controller controls the corresponding collecting valve 6 according to the set parameters to collect the specific secondary separation component, as shown in figure 4.
In one embodiment, the gradient elution unit comprises a first gradient pump, a second gradient pump and a first mixer, wherein the first gradient pump and the second gradient pump are both connected with the inlet of the first mixer, the outlet of the first mixer is connected with a first two-position four-way valve 2, the first gradient pump and the second gradient pump are both in communication connection with the signal output end of the controller, and the first mixer is a static mixer or a dynamic mixer. The first mixer is used for mixing elution reagents pumped by the first gradient pump and the second gradient pump to obtain eluent, wherein the elution reagents are water, methanol or ethanol, the controller controls the three-way valve 1 to enable the three-way valve 1 to be communicated with the first two-position four-way valve 2 to enable an outlet of the first mixer to be communicated with an inlet of the separation unit, the controller controls the third two-position four-way valve 4 to enable all separation columns to be not communicated, the controller controls the fourth two-position four-way valve 5 to enable all trapping columns to be not communicated, the controller controls the second two-position four-way valve 3 to enable an outlet of the separation unit to be communicated with an inlet of the second mixer, an outlet of the second mixer is communicated with an inlet of the trapping unit through the first two-position four-way valve 2, an outlet of the trapping unit is communicated with the waste liquid valve 7 in the collection unit through the second two-way valve 3, and then the controller controls the first gradient pump and the second gradient pump to run at equal degrees to enable the whole system to be stable in state, the figure 1 is shown, the controller controls the first gradient pump to start sampling pump, and after sample injection is finished, the gradient pump and the gradient pump is controlled by the controller to elute the sample injection.
In one embodiment, the first two-position four-way valve 2, the second two-position four-way valve 3, the third two-position four-way valve 4, the fourth two-position four-way valve 5 and the three-way valve 1 are all solenoid valves and have a pressure resistance of 20MPa, and the collecting valve 6 is an air filling valve.
In one embodiment, the packing in the separation column comprises one of reverse phase packing, ion exchange packing, molecular sieve packing, and affinity chromatography packing; the packing in the trap column comprises one of reversed phase packing and polymer hydrophobic resin packing.
Wherein, the sample injection pump, the first gradient pump, the second gradient pump and the dilution pump are all constant flow pumps, the pressure resistance range is between 0 and 25MPa, the flow rate range is between 200 and 2000mL/min, and the flow rate of the dilution pump is 1 to 10 times of the flow rate of the first gradient pump and the second gradient pump.
The two-dimensional liquid chromatography separation system is suitable for separating complex components, is particularly suitable for separating components with low content, thermal sensitivity, light sensitivity and easy oxidation, and has the advantages of low reagent dosage in the separation process, environmental protection, high purity of the separated product and high recovery rate.
The two-dimensional liquid chromatography separation system can realize synchronous high-efficiency separation of active ingredients such as nicotine, solanone, chlorogenic acid and rutin in the waste green tobacco leaves, and promotes diversified utilization of the waste green tobacco leaves.
The working method of the two-dimensional liquid chromatography separation system comprises the following steps:
s1, as shown in figure 1, before sample introduction, a controller controls a three-way valve 1 to enable the three-way valve 1 to be communicated with a first two-position four-way valve 2 so as to enable an outlet of a first mixer to be communicated with an inlet of a separation unit, controls a third two-position four-way valve 4 to enable each separation column to be not communicated, controls a fourth two-position four-way valve 5 to enable each trapping column to be not communicated, controls a second two-position four-way valve 3 to enable an outlet of the separation unit to be communicated with an inlet of a second mixer, enables an outlet of the second mixer to be communicated with an inlet of the trapping unit through the first two-position four-way valve 2, enables an outlet of the trapping unit to be communicated with a waste liquid valve 7 in a collecting unit through the second two-position four-way valve 3, then the controller controls the first gradient pump and the second gradient pump to start, the first gradient pump and the second gradient pump respectively pump elution reagents with the same concentration and mix the same through the first mixer to obtain equal-concentration eluent, the equal-concentration eluent sequentially passes through the first two-position four-way valve 2, the three-way valve 1, each third two-position four-way valve 4, the second two-position four-way valve 3, the second mixer, the first two-position four-way valve 2 and each fourth two-position four-way valve 5 through connecting pipelines and passes through the waste liquid valve 7 to be collected, so that the equal-degree operation is realized, and the state of the whole system is stable;
s2, as shown in the figure 2, the controller controls the three-way valve 1 to enable the three-way valve 1 to be communicated with the sample injection pump, controls the sample injection pump, and injects a sample to be separated into one of the separation columns of the separation unit;
s3, as shown in fig. 3, in the one-dimensional separation mode, the controller controls the three-way valve 1 to communicate the three-way valve 1 with the first two-position four-way valve 2, so that the outlet of the first mixer is communicated with the inlet of the separation unit, then the controller controls the first gradient pump and the second gradient pump to start, the first gradient pump and the second gradient pump respectively pump the elution reagents with different concentrations, and mix them by the first mixer to obtain a gradient eluent, and perform gradient elution separation on the sample to be separated in the corresponding separation column by the gradient eluent, each one-level separation component in the sample to be separated is sequentially eluted and separated, the detector performs real-time detection on the one-level separation component in the corresponding separation column, and feeds back the detection result to the controller, the controller controls the corresponding fourth two-position four-way valve 5 according to the set parameters, so as to trap the specific one-level separation component diluted by the diluent pumped by the diluent pump by the corresponding trapping column, thus one-dimensional separation of each one-level separation component in the sample to be separated can be realized, and the eluate flowing out from the corresponding trapping column passes through the waste liquid valve 7 to be collected as waste liquid, wherein the purity of each one-level separation component is more than 80%;
s4, because each primary separation component captured by the capture column is still a mixture, each primary separation component needs to be respectively subjected to two-dimensional separation, as shown in figure 4, in a two-dimensional separation mode, the controller controls the first two-position four-way valve 2 to enable the outlet of the first mixer to be communicated with the inlet of the capture unit, the controller controls the second two-position four-way valve 3 to enable the outlet of the capture unit to be communicated with the inlet of the second mixer, the outlet of the second mixer is communicated with the inlet of the separation unit through the first two-position four-way valve 2 and the three-way valve 1 in sequence, the outlet of the separation unit is communicated with the collection unit through the second two-position four-way valve 3, then the controller controls the first gradient pump and the second gradient pump to be started, so that a certain primary separation component captured by the corresponding capture column is eluted through gradient eluent, the primary separation component is eluted and diluted by diluent along with the gradient and then is fed back to the corresponding separation column, the detector performs real-time detection on the secondary separation component in the effluent of the corresponding separation column, the sample injection controller controls the collection valve to perform effective concentration on the secondary separation component to obtain more than 95% of the collected fraction.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and replacements can be made without departing from the technical principle of the present invention, and these modifications and replacements should also be regarded as the protection scope of the present invention.
Claims (10)
1. A two-dimensional liquid chromatography separation system, comprising: comprises a sample introduction unit, a gradient elution unit, a separation unit, a detector, a trapping unit and a collection unit; the sample introduction unit is connected with the inlet of the separation unit; the gradient elution unit is respectively connected with inlets of the separation unit and the capture unit through a first two-position four-way valve (2), the collection unit is respectively connected with outlets of the separation unit and the capture unit through a second two-position four-way valve (3), and a detector is arranged between the separation unit and the second two-position four-way valve (3); and the system is switched from a one-dimensional separation mode to a two-dimensional separation mode by switching the first two-position four-way valve (2) and the second two-position four-way valve (3).
2. A two-dimensional liquid chromatography separation system according to claim 1, wherein: still include the controller, detector and controller signal input end communication connection, advance kind unit, gradient elution unit, separation element, entrapment unit and collection unit and all with controller signal output end communication connection.
3. A two-dimensional liquid chromatography separation system according to claim 2, wherein: the device also comprises a dilution unit, wherein the dilution unit comprises a dilution pump and a second mixer, the dilution pump and a second two-position four-way valve (3) are both connected with the inlet of the second mixer, and the outlet of the second mixer is connected with a first two-position four-way valve (2); the dilution pump is in communication connection with the signal output end of the controller.
4. A two-dimensional liquid chromatography separation system according to claim 2, wherein: the sampling unit includes sampling pump and three-way valve (1), the sampling pump passes through three-way valve (1) and the detaching unit entry linkage, first two-position cross valve (2) are connected with three-way valve (1), sampling pump, three-way valve (1) and first two-position cross valve (2) all with controller signal output part communication connection.
5. A two-dimensional liquid chromatography separation system according to claim 4, wherein: the separation unit is formed by connecting a plurality of separation columns in parallel through a third two-position four-way valve (4), and one of the separation columns is conducted at the same separation moment; the trapping unit is formed by connecting a plurality of trapping columns in parallel through a fourth two-position four-way valve (5), and one of the trapping columns is conducted at the same separation moment; and the third two-position four-way valve (4) and the fourth two-position four-way valve (5) are in communication connection with the signal output end of the controller.
6. A two-dimensional liquid chromatography separation system according to claim 2, wherein: the collecting unit comprises a plurality of collecting valves (6) and a waste liquid valve (7), the collecting valves (6) and the waste liquid valve (7) are connected in parallel, and one of the collecting valves (6) is communicated at the same separation moment; the collection valve (6) is in communication connection with the signal output end of the controller.
7. A two-dimensional liquid chromatography separation system according to claim 2, wherein: the gradient elution unit comprises a first gradient pump, a second gradient pump and a first mixer, wherein the first gradient pump and the second gradient pump are both connected with the inlet of the first mixer, and the outlet of the first mixer is connected with a first two-position four-way valve (2); and the first gradient pump and the second gradient pump are both in communication connection with the signal output end of the controller.
8. A two-dimensional liquid chromatography separation system according to claim 6, wherein: the pneumatic-control system is characterized in that the first two-position four-way valve (2), the second two-position four-way valve (3), the third two-position four-way valve (4), the fourth two-position four-way valve (5) and the three-way valve (1) are all electromagnetic valves, and the collecting valve (6) is an air filling valve.
9. A two-dimensional liquid chromatography separation system according to claim 5, wherein: the filler in the separation column comprises one of reversed phase filler, ion exchange filler, molecular sieve filler and affinity chromatography filler.
10. A two-dimensional liquid chromatography separation system according to claim 5, wherein: the filler in the trapping column comprises one of reversed phase filler and polymer hydrophobic resin filler.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221904045.8U CN217981369U (en) | 2022-07-22 | 2022-07-22 | Two-dimensional liquid chromatography separation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221904045.8U CN217981369U (en) | 2022-07-22 | 2022-07-22 | Two-dimensional liquid chromatography separation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217981369U true CN217981369U (en) | 2022-12-06 |
Family
ID=84280127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221904045.8U Active CN217981369U (en) | 2022-07-22 | 2022-07-22 | Two-dimensional liquid chromatography separation system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217981369U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116203168A (en) * | 2023-04-28 | 2023-06-02 | 广东省农业科学院蚕业与农产品加工研究所 | Circulation type multidimensional liquid chromatograph and application thereof |
-
2022
- 2022-07-22 CN CN202221904045.8U patent/CN217981369U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116203168A (en) * | 2023-04-28 | 2023-06-02 | 广东省农业科学院蚕业与农产品加工研究所 | Circulation type multidimensional liquid chromatograph and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104713973B (en) | There is two-dimentional preparative hplc instrument system and the application thereof of on-line preconcentration function | |
| CN102580352B (en) | Separation method of natural product | |
| CN107121504B (en) | Multi-functional temperature-pressure extraction-trapping-chromatographic isolation on-line coupling equipment | |
| CN217981369U (en) | Two-dimensional liquid chromatography separation system | |
| CN103235074B (en) | High performance liquid chromatography online analysis method and application thereof | |
| CN105606753B (en) | Field auxiliary extraction-separation and concentration-chromatography on-line coupling equipment | |
| CN101718753A (en) | Ion chromatography single-pump column switching system | |
| CN102961892A (en) | Preparative multidimensional liquid chromatographic instrument | |
| CN207586196U (en) | The multidimensional liquid chromatographic separation system of full on-line checking based on same detector | |
| CN109682920A (en) | A kind of system and method for the online standard curve making of ion chromatography | |
| CN111735685A (en) | Full-automatic nuclide solid-phase extraction device and extraction method | |
| CN101619090A (en) | Column-switching circulating volume exclusion chromatography system for biomacromolecule separation and purification | |
| CN101791491A (en) | Online low-pressure, middle-pressure and high-pressure combined two-dimension preparation liquid chromatographic system | |
| CN109499095A (en) | Full-automatic element sepatation purification devices | |
| CN219308758U (en) | Microfluidic protein purification core, detection system and double-purification unit chip | |
| CN100554927C (en) | A kind of automatic flow-path separation device | |
| CN104678014B (en) | A kind of Two-dimensional Liquid Chromatography | |
| CN113341030B (en) | High-flux liquid chromatography-mass spectrometry system and separation and analysis method | |
| CN2832294Y (en) | Automatic flow path separating device | |
| CN205435056U (en) | Chromatographically pure piece -rate system | |
| CN209327285U (en) | A kind of circulatory system of high performance liquid chromatograph mobile phase | |
| CN101620041B (en) | Automatic flow-path separation device | |
| CN101406767B (en) | Compound separating mechanism | |
| CN114392583A (en) | Chromatographic purification system and purification method | |
| CN201324543Y (en) | Chemical compound separating device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |