CN217981260U - micro-Raman imaging device - Google Patents

micro-Raman imaging device Download PDF

Info

Publication number
CN217981260U
CN217981260U CN202121413076.9U CN202121413076U CN217981260U CN 217981260 U CN217981260 U CN 217981260U CN 202121413076 U CN202121413076 U CN 202121413076U CN 217981260 U CN217981260 U CN 217981260U
Authority
CN
China
Prior art keywords
mirror
light
collimating mirror
objective table
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202121413076.9U
Other languages
Chinese (zh)
Inventor
李静婷
赵俊洋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Bluefin Technology Co ltd
Original Assignee
Tianjin Bluefin Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Bluefin Technology Co ltd filed Critical Tianjin Bluefin Technology Co ltd
Priority to CN202121413076.9U priority Critical patent/CN217981260U/en
Application granted granted Critical
Publication of CN217981260U publication Critical patent/CN217981260U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The utility model discloses a micro-raman imaging device relates to raman spectrum detection technical field, including objective table, microscope and CCD camera, the microscope sets up in the top of objective table, microscopical top is provided with the second slide, the top of second slide is provided with the third collimating mirror, the third collimating mirror sets up in the below of CCD camera, the outside of second slide is provided with the second mirror, one side that the second mirror was kept away from to the second slide is provided with the fourth collimating mirror, one side that the second slide was kept away from to the fourth collimating mirror is provided with the third reflector, the below of third reflector is provided with the beam expanding mirror, the below of beam expanding mirror is provided with the ware that divides, the below of ware that divides is provided with the light source, improve the definition that the CCD camera caught the image, simultaneously through the multiunit slide, the assistance of collimating mirror and reflector, be convenient for catch sample structure, effectual improvement spectral analysis's the degree of accuracy.

Description

micro-Raman imaging device
Technical Field
The utility model relates to a raman spectroscopy detects technical field, specifically is a micro-raman imaging device.
Background
Raman spectroscopy is an important spectroscopic technique for studying the microstructure of substances. The Raman spectrometer is mainly used for judging and confirming research material components, and can also be applied to drug detection and gem identification in criminal investigation and jewelry industries. The raman spectrometer adopts a laser as a light source, is harmful to human bodies, and needs to wear protective glasses when the raman spectrometer is used. And when measuring the spectrum of a sample, the same sample needs to be measured at multiple points.
The traditional microscope can realize the observation of tiny objects, but the objective objects are observed subjectively through the eyepieces and focusing is carried out through the manual adjusting knob, and the manual focusing mode is time-consuming, labor-consuming and easy to produce manual errors. In addition, due to the loss of the light source in the raman spectrometer and the influence of the light of the microscope stage, the image acquired by the camera is not clear enough, and the sensitivity of detection induction of the raman spectrum is reduced.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a micro-raman imaging device solves the problem that the image acquisition is unclear and spectral detection sensitivity descends through installation multiunit beam splitter, collimating mirror and speculum.
In order to achieve the above object, the utility model provides a following technical scheme: a micro-Raman imaging device comprises an object stage, a microscope and a CCD camera, wherein the microscope is arranged above the object stage, a second light splitter is arranged above the microscope, a third collimating mirror is arranged above the second light splitter, the third collimating mirror is arranged below the CCD camera, a second reflecting mirror is arranged outside the second light splitter, a fourth collimating mirror is arranged on one side, away from the second reflecting mirror, of the second light splitter, a third reflecting mirror is arranged on one side, away from the second light splitter, of the fourth collimating mirror, a beam expanding mirror is arranged below the third reflecting mirror, a divider is arranged below the second reflecting mirror, a light source is arranged below the divider, a first light splitter is arranged below the second light splitter, a first reflecting mirror is arranged below the first light splitter, a first collimating mirror is arranged outside the first light splitter, a second optical fiber probe is arranged on one side, away from the first light splitter, an optical filter is arranged outside the first reflecting mirror, and a second optical fiber probe is arranged on one side, away from the second collimating mirror.
Preferably, a microscopic imaging tube is arranged on the outer side of the microscope, and an observation mirror is arranged above the microscopic imaging tube.
Preferably, a base is installed below the object stage, and a bottom plate is fixed below the base.
Preferably, a raman spectrometer is fixed above the bottom plate, a display screen is installed on the outer side of the raman spectrometer, and a control key is installed on the outer side of the raman spectrometer.
Preferably, a threaded rod penetrates through the inner side of the object stage, a rotating head is fixed at the top of the threaded rod, and the bottom of the threaded rod penetrates through the base.
Preferably, a first sliding groove is formed in the inner side of the object stage, a second sliding groove is formed in the inner side of the base, an X-shaped telescopic rod is installed on the outer sides of the object stage and the base, and the X-shaped telescopic rod penetrates through the first sliding groove and the second sliding groove.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the light source is opened, the light source is dispersed into light rays adaptive to the beam expander through the divider, the light rays are irradiated onto the third reflector after being expanded by the beam expander, the light rays reflected by the third reflector are irradiated onto the second light splitting sheet, one part of the light rays are irradiated downwards through reflection and penetrate through the microscope and are irradiated above the objective table, so that a sample above the objective table is reflected in the form of image light rays and penetrates through the microscope, the light rays are condensed through the second light splitting sheet and the third collimating lens, an image is captured through the CCD camera, the other part of the light rays and the image of the sample are reflected through the second light splitting sheet and the second light splitting sheet, after the light rays are split through the first light splitting sheet, one part of the light rays are captured through the first collimating lens and are captured through the second optical fiber probe, the other part of the light rays are reflected through the first reflector, after being filtered through the optical filter and condensed through the second collimating lens, the light rays are captured through the first optical fiber probe, so that Raman spectrum analysis is performed through the display screen, the utility model discloses a light ray source provides light rays, the definition of the captured of the image of the CCD camera is improved, and the sample structure is conveniently detected through the multiple groups of the light splitting sheet, and the sample structure is effectively improved in spectrum analysis;
2. rotatory rotating head drives the threaded rod and is rotatory in the inboard of objective table to drive the objective table and go up and down in the outside of threaded rod, through the lift of installation X type telescopic link auxiliary objective table, the steadiness when being convenient for improve the objective table and go up and down, thereby the image of the sample of being convenient for and the seizure of spectrum.
Drawings
Fig. 1 is a perspective view of the present invention;
FIG. 2 is a schematic structural view of the present invention;
fig. 3 is a three-dimensional structure diagram of the objective table of the present invention.
In the figure: 1-Raman spectrometer, 2-display screen, 3-control button, 4-bottom plate, 5-base, 6-objective table, 7-microscope, 8-microscopic imaging tube, 9-observation mirror, 10-first optical fiber probe, 11-second optical fiber probe, 12-first collimating mirror, 13-second collimating mirror, 14-optical filter, 15-first reflector, 16-first light splitter, 17-second reflector, 18-second light splitter, 19-third collimating mirror, 20-CCD camera, 21-fourth collimating mirror, 22-third reflector, 23-beam expander, 24-beam splitter, 25-light source, 26-threaded rod, 27-rotating head, 28-first chute, 29-second chute and 30-X type telescopic rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides an embodiment: a micro-Raman imaging device comprises an object stage 6, a microscope 7 and a CCD camera 20, wherein the microscope 7 is arranged above the object stage 6, a second light splitting lens 18 is arranged above the microscope 7, a third collimating lens 19 is arranged above the second light splitting lens 18, the third collimating lens 19 is arranged below the CCD camera 20, a second reflecting lens 17 is arranged outside the second light splitting lens 18, a fourth collimating lens 21 is arranged on one side, away from the second reflecting lens 17, of the second light splitting lens 18, a third reflecting lens 22 is arranged on one side, away from the second light splitting lens 18, of the fourth collimating lens 21, a beam expanding lens 23 is arranged below the third reflecting lens 22, a divider 24 is arranged below the beam expanding lens 23, a light source 25 is arranged below the divider 24, a first light splitting lens 16 is arranged below the second reflecting lens 17, a first reflecting lens 15 is arranged below the first light splitting lens 16, a first collimating lens 12 is arranged outside the first light splitting lens 16, a second probe optical fiber 11 is arranged on one side, away from the first light splitting lens 15, a first collimating lens 14 is arranged outside the first light splitting lens 12, and a second collimating lens 13 is arranged on one side, away from the second light splitting lens 14.
The light source 25 is turned on, the light source is dispersed into light rays adaptive to the beam expander 23 through the divider 24, the light rays are expanded by the beam expander 23 and then irradiate on the third reflector 22, the light rays reflected by the third reflector 22 irradiate on the second light splitting plate 18, one part of the light rays are downward irradiated through reflection and pass through the microscope 7 and irradiate above the objective table 6, so that a sample above the objective table 6 passes through the microscope 7 in an image light ray mode in a reflected mode, after being condensed by the second light splitting plate 18 and the third collimator 19, an image is captured through the CCD camera 20, the other part of the light rays and the image of the sample are reflected through the second light splitting plate 18 and the second reflector 17, after being split by the first light splitting plate 16, one part of the light rays are condensed by the first collimating lens 12 and captured by the second optical fiber probe 11, and the other part of the light rays are reflected by the first reflector 15, are filtered by the optical filter 14 and condensed by the second collimating lens 13 and captured by the first optical fiber probe 10, and then Raman spectrum analysis is performed through the display screen 2.
In this embodiment, a microscope imaging tube 8 is attached to the outside of the microscope 7, and an observation mirror 9 is attached to the top of the microscope imaging tube 8.
The observation mirror 9 is convenient for observing the microstructure of the sample intuitively and conveniently, and the microscopic imaging tube 8 is convenient for installing a microscopic imaging system.
In this embodiment, a base 5 is attached below the stage 6, and a bottom plate 4 is fixed below the base 5.
By fixing the base 5 above the bottom plate 4, the stability of the device is effectively improved.
In this embodiment, a raman spectrometer 1 is fixed above the bottom plate 4, a display screen 2 is installed outside the raman spectrometer 1, and a control key 3 is installed outside the raman spectrometer 1.
The spectrometer is set or adjusted by the control key 3 and the detection result is displayed on the display screen 2.
In this embodiment, a threaded rod 26 is inserted into the stage 6, a rotary head 27 is fixed to the top of the threaded rod 26, and the bottom of the threaded rod 26 is inserted into the base 5.
The rotary swivel 27 rotates the threaded rod 26 inside the stage 6, and thereby moves the stage 6 up and down outside the threaded rod 26.
In this embodiment, a first sliding groove 28 is formed on the inner side of the stage 6, a second sliding groove 29 is formed on the inner side of the base 5, an X-shaped telescopic rod 30 is mounted on the outer sides of the stage 6 and the base 5, and the X-shaped telescopic rod 30 penetrates through the first sliding groove 28 and the second sliding groove 29.
When the objective table 6 goes up and down, the X-shaped telescopic rod 30 is driven to slide and stretch in the inner sides of the first sliding groove 28 and the second sliding groove 29, so that the objective table 6 is stably lifted and lowered, and the stability is improved.
The working principle is as follows:
step 1: firstly, the light source 25 is turned on, the light source is dispersed into light rays adaptive to the beam expander 23 through the divider 24, the light rays are expanded by the beam expander 23 and then irradiate on the third reflector 22, the light rays reflected by the third reflector 22 irradiate on the second light splitting plate 18, one part of the light rays are downward irradiated through reflection and irradiate above the objective table 6 through the microscope 7, so that a sample above the objective table 6 is reversely irradiated in the form of image light rays and passes through the microscope 7, the light rays are condensed through the second light splitting plate 18 and the third collimator 19 and then are captured by the CCD camera 20, the other part of the light rays and the image of the sample are reflected through the second light splitting plate 18 and the second reflector 17, after the light rays are split through the first light splitting plate 16, one part of the light rays are condensed by the first collimating lens 12 and are captured by the second optical fiber probe 11, the other part of the light rays are reflected by the first reflector 15, the light rays are filtered by the optical filter 14 and then are condensed by the second collimating lens 13 and then are captured by the first optical probe 10, so that the raman spectrum analysis is performed through the display screen 2, the light spectrum analysis accuracy is improved through the CCD camera, and the auxiliary light beam structure of the CCD camera is improved;
step 2: secondly, rotatory rotating head 27 drives threaded rod 26 and is rotatory in the inboard of objective table 6 to drive objective table 6 and go up and down in the outside of threaded rod 26, through the lift of installation X type telescopic link 30 auxiliary objective table 6, the steadiness when being convenient for improve objective table 6 and go up and down, thereby the image of the sample of being convenient for and the seizure of spectrum.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. A micro-Raman imaging apparatus comprising an object stage (6), a microscope (7) and a CCD camera (20), characterized in that: the microscope (7) is arranged above the objective table (6), a second light splitter (18) is arranged above the microscope (7), a third collimating mirror (19) is arranged above the second light splitter (18), the third collimating mirror (19) is arranged below the CCD camera (20), a second reflecting mirror (17) is arranged outside the second light splitter (18), a fourth collimating mirror (21) is arranged on one side, far away from the first reflecting mirror (17), of the second light splitter (18), a third reflecting mirror (22) is arranged on one side, far away from the second light splitter (18), of the fourth collimating mirror (21), a beam expander (23) is arranged below the third reflecting mirror (22), a divider (24) is arranged below the beam expander (23), a light source (25) is arranged below the divider (24), a first light splitter (16) is arranged below the first reflecting mirror (17), a first reflecting mirror (15) is arranged below the first light splitter (16), a first light filter (12) is arranged outside the collimating mirror (12), and a first optical fiber (14) is arranged outside the collimating mirror (11), and a second collimating mirror (13) is arranged on one side of the optical filter (14) far away from the first reflecting mirror (15), and a first optical fiber probe (10) is arranged on one side of the second collimating mirror (13) far away from the optical filter (14).
2. A micro-raman imaging device according to claim 1, characterized in that: a microscopic imaging tube (8) is installed on the outer side of the microscope (7), and an observation mirror (9) is installed above the microscopic imaging tube (8).
3. A micro-raman imaging device according to claim 1, characterized in that: a base (5) is installed below the objective table (6), and a bottom plate (4) is fixed below the base (5).
4. A micro-raman imaging device according to claim 3, characterized in that: a Raman spectrometer (1) is fixed above the bottom plate (4), a display screen (2) is installed on the outer side of the Raman spectrometer (1), and a control key (3) is installed on the outer side of the Raman spectrometer (1).
5. A micro-raman imaging device according to claim 3, characterized in that: the inboard of objective table (6) is run through and is installed threaded rod (26), the top of threaded rod (26) is fixed with rotatory head (27), the bottom of threaded rod (26) is run through base (5).
6. A micro-raman imaging device according to claim 3, characterized in that: first spout (28) have been seted up to the inboard of objective table (6), second spout (29) have been seted up to the inboard of base (5), objective table (6) with X type telescopic link (30) are installed in the outside of base (5), X type telescopic link (30) run through first spout (28) with second spout (29).
CN202121413076.9U 2021-06-24 2021-06-24 micro-Raman imaging device Active CN217981260U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202121413076.9U CN217981260U (en) 2021-06-24 2021-06-24 micro-Raman imaging device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202121413076.9U CN217981260U (en) 2021-06-24 2021-06-24 micro-Raman imaging device

Publications (1)

Publication Number Publication Date
CN217981260U true CN217981260U (en) 2022-12-06

Family

ID=84255011

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202121413076.9U Active CN217981260U (en) 2021-06-24 2021-06-24 micro-Raman imaging device

Country Status (1)

Country Link
CN (1) CN217981260U (en)

Similar Documents

Publication Publication Date Title
US6870612B2 (en) Portable spectral imaging microscope system
JP6283104B2 (en) Optical analyzer
CN104390943B (en) It is a kind of while obtaining the micro imaging system of appearance images and Elemental redistribution image
CN107884388A (en) A kind of micro-Raman spectroscopy and its application method of fast automatic focusing
WO2017221024A1 (en) Apparatus and method for extracting low intensity photonic signals
WO2018075562A1 (en) Imaging platform based on nonlinear optical microscopy for rapid scanning large areas of tissue
IL296784B2 (en) Imaging assisted scanning spectroscopy for gem identification
Patil et al. A handheld laser scanning confocal reflectance imaging–confocal Raman microspectroscopy system
WO2016063322A1 (en) Optical analysis device and biomolecular analysis device
US20180246083A1 (en) System, method and computer readable medium for imaging large areas with microscopic resolution
Yeh et al. Discrete frequency infrared imaging using quantum cascade lasers for biological tissue analysis
CN217981260U (en) micro-Raman imaging device
KR20100002742A (en) Method for imaging raman spectroscopy of biologic tissues and cells and an apparatus using the same
CN204269547U (en) A kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image
Dadkhah et al. Optical coherence tomography-guided dynamic focusing for combined optical and mechanical scanning multimodal photoacoustic microscopy
CN104267488A (en) Optical microscope beam splitter device
US20210164905A1 (en) Method and apparatus for simultaneous nonlinear excitation and detection of different chromophores across a wide spectral range using ultra-broadband light pulses and time-resolved detection
CN203824908U (en) Laser-induced breakdown spectroscopy elemental analyzer capable of positioning target spots accurately
CN109187502A (en) Postposition is divided pupil confocal laser LIBS spectrum micro imaging method and device
CN109187723A (en) Postposition is divided pupil differential confocal Raman spectra-mass spectrum micro imaging method and device
CN109211874A (en) Postposition is divided pupil confocal laser Raman spectra test method and device
Breunig et al. Rapid vertical tissue imaging with clinical multiphoton tomography
US6744572B1 (en) System and method for imaging an object
US20180271368A1 (en) Device for determining a condition of an organ and method of operating the same
RU162395U1 (en) TWO PHOTON SCANNING MICROSCOPE

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant