CN217919088U

CN217919088U - Whole blood DNA extraction kit adopting paramagnetic particle method

Info

Publication number: CN217919088U
Application number: CN202222377681.6U
Authority: CN
Inventors: 邹春平; 郭陆军; 鲁明霞; 张义强; 刘洪�
Original assignee: Henan Taifu Medical Laboratory Center Co ltd
Current assignee: Henan Taifu Medical Laboratory Center Co ltd
Priority date: 2022-09-07
Filing date: 2022-09-07
Publication date: 2022-11-29
Anticipated expiration: 2032-09-07

Abstract

The utility model relates to a whole blood DNA extraction kit by a magnetic bead method, comprising a box body and a box cover, wherein a first clapboard and a second clapboard are fixedly arranged in the box body in a staggered way, the box body is divided into a plurality of EP reagent tube placing areas by the first clapboard and the second clapboard, each placing area is internally provided with a split type positioning component for placing EP reagent tubes, before transportation, each split type positioning component provided with the EP reagent tubes is respectively embedded into the corresponding placing area, each EP reagent tube can be effectively isolated and protected by the split type positioning component, thereby preventing the EP reagent tubes from shaking violently in the transportation process; adopt split type locating component still can make things convenient for the staff to carry out multiple mode selection to the EP reagent pipe quantity that the single was taken, can the single one of taking, two, four or eight, simple structure, the convenience of taking.

Description

Whole blood DNA extraction kit by magnetic bead method

Technical Field

The utility model relates to the field of biotechnology, especially, relate to magnetic bead method whole blood DNA draws kit.

Background

Genomic DNA contains all the genetic information of cells, and extraction of DNA from whole blood is a simple method for obtaining biological genomic DNA and is also an important technology for gene correlation studies. The yield of the DNA extraction quality box directly influences the accuracy of subsequent experiments, and the magnetic bead method belongs to a solid phase extraction method and is a separation technology adopting magnetic beads as carriers. Because the magnetic bead method is simple and rapid in extraction and accounting operation and good in repeatability, DNA and RNA can be separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like, and the kit can be applied to various fields such as a disease control center, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like, but the current DNA extraction kit cannot effectively fix an EP reagent tube, and the EP reagent tube filled with the extracted sample is easy to shake in the transportation process and cannot meet the requirements.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a magnetic bead method whole blood DNA draws kit for solve current DNA and draw the kit and not only can't carry out effectual fixing to EP reagent pipe, the EP reagent pipe that is equipped with the extraction sample appears the problem of rocking easily in the in-process of transportation.

In order to solve the problems, the novel whole blood DNA extraction kit adopting the magnetic bead method comprises a kit body and a kit cover, wherein a first partition plate and a second partition plate are fixedly arranged in the kit body in a staggered mode, the kit body is divided into a plurality of EP reagent tube placing areas by the first partition plate and the second partition plate, and each EP reagent tube placing area is internally provided with a split type positioning assembly for placing an EP reagent tube.

The utility model provides a magnetic bead method whole blood DNA draws kit still has following technical characteristic:

further, the first partition and the second partition are perpendicular to each other.

Furthermore, the split type positioning assembly comprises a first positioning block and a second positioning block butted with the first positioning block, a positioning groove is formed in one side of the first positioning block, the positioning groove is a square groove, and a convex edge matched with the positioning groove is convexly arranged on one surface of the second positioning block butted with the first positioning block.

Further, the overall shape of the first positioning block or the second positioning block is triangular prism-shaped.

Furthermore, the heights of the first positioning block and the second positioning block are the same, and the lengths of two right-angle sides of the first positioning block or the second positioning block are equal.

Furthermore, EP reagent tube placing holes are formed in the tops of the first positioning block and the second positioning block, and the EP reagent tube placing holes are blind holes.

Further, the depth of the EP reagent tube placing hole is larger than or equal to half of the height of the first positioning block.

Furthermore, the first positioning block and the second positioning block are butted to form a whole body in a square shape.

Furthermore, the first positioning block and the second positioning block are both made of rubber.

Furthermore, handles are fixedly arranged at the tops of the first positioning block and the second positioning block.

The utility model discloses following beneficial effect has: before transportation, each split type positioning assembly provided with the EP reagent tubes is embedded into the corresponding placement area, and each EP reagent tube can be effectively isolated and protected by the split type positioning assembly, so that the EP reagent tubes are prevented from shaking violently in the transportation process; adopt split type locating component still can make things convenient for the staff to carry out multiple mode selection to the EP reagent pipe quantity that the single was taken, can the single one of taking, two, four or eight, simple structure, the convenience of taking.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the whole blood DNA extraction kit by the paramagnetic particle method;

FIG. 2 is a schematic diagram of the whole blood DNA extraction kit by the paramagnetic particle method after being opened;

FIG. 3 is a schematic view of the overall structure of the split positioning assembly after splicing;

FIG. 4 is a schematic structural diagram of the first positioning block;

FIG. 5 is a schematic structural diagram of the second positioning block;

fig. 6 is a schematic structural view after the split positioning assembly is completely taken out.

Detailed Description

The invention will be described in detail hereinafter with reference to the drawings and with reference to embodiments, it being understood that the embodiments and features of the embodiments can be combined without conflict.

As shown in fig. 1 to fig. 6, in one embodiment of the whole blood DNA extraction kit by magnetic bead method of the present invention, including a box body 1 and a box cover 2, a first partition plate 3 and a second partition plate 4 are fixedly arranged in the box body 1 in a staggered manner, the box body 1 is divided into a plurality of EP reagent tube placement areas 101 by the first partition plate 3 and the second partition plate 4, and each EP reagent tube placement area 101 is provided with a split positioning component 5 for placing an EP reagent tube 100, specifically, before transportation, each split positioning component 5 provided with an EP reagent tube 100 is first embedded into the corresponding placement area 101, and each EP reagent tube 100 can be effectively isolated and protected by the split positioning component 5, so as to prevent the EP reagent tube 100 from shaking violently during transportation; adopt split type locating component 5 still can make things convenient for the staff to carry out multiple mode selection to the number of the EP reagent pipe 5 that the single was taken, can take one, two, four or eight by the single, simple structure, the convenience of taking.

In one embodiment of the present application, the first partition board 3 and the second partition board 4 are perpendicular to each other, specifically, the first partition board 3 and the second partition board 4 are perpendicular to each other to form a cross frame, and the cassette body 1 is divided into 4 EP reagent tube placing regions 101 by the first partition board 3 and the second partition board 4.

In an embodiment of the present application, the split type positioning assembly 5 includes a first positioning block 51 and a second positioning block 52 butted with the first positioning block 51, a positioning groove 510 is formed on one side of the first positioning block 51, the positioning groove 510 is a square groove, and a convex rib 521 matched with the positioning groove 510 is convexly disposed on a surface of the second positioning block 52 butted with the first positioning block 51.

In one embodiment of the present application, the overall shape of the first positioning block 51 or the second positioning block 52 is a triangular prism.

In one embodiment of the present application, the heights of the first positioning block 51 and the second positioning block 52 are the same, and the lengths of the two perpendicular sides of the first positioning block 51 or the second positioning block 52 are equal.

In an embodiment of the application, the EP reagent tube placing holes 50 are formed in the tops of the first positioning block 51 and the second positioning block 52, the EP reagent tube placing holes 50 are blind holes, specifically, the bottom end of the EP reagent tube 100 abuts against the bottom wall of the EP reagent tube placing holes 50, and the top end of the EP reagent tube 100 is 2cm or 3cm higher than the top end of the EP reagent tube placing holes 100, so that a worker can conveniently pull out the EP reagent tube 100 from the EP reagent tube placing holes 50.

In one embodiment of the present application, the depth of the EP reagent tube placing hole 50 is greater than or equal to half the height of the first positioning block 51.

In one embodiment of the present application, the first positioning block 51 and the second positioning block 52 are butted and are entirely shaped like a cube.

In an embodiment of the present application, the first positioning block 51 and the second positioning block 52 are both made of rubber, and specifically, the EP reagent tube 100 can be protected by buffering by using a rubber material.

In one embodiment of the present application, a handle 6 is fixedly disposed on the top of each of the first positioning block 51 and the second positioning block 52.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or the central technical features thereof may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. Magnetic bead method whole blood DNA draws kit, including box body and lid, its characterized in that: the utility model discloses a reagent bottle box, including box body, EP reagent pipe places the district, every EP reagent pipe places the district and all is equipped with the split type locating component that is used for placing EP reagent pipe in the district, crisscross fixed first baffle and the second baffle of being equipped with in the box body, the box body is divided into a plurality of EP reagent pipe places the district with the second baffle, every EP reagent pipe places the district.

2. The kit for extracting DNA from whole blood by the magnetic bead method according to claim 1, wherein: the first partition plate and the second partition plate are perpendicular to each other.

3. The kit for extracting DNA from whole blood by the magnetic bead method according to claim 2, wherein: the split type positioning assembly comprises a first positioning block and a second positioning block butted with the first positioning block, a positioning groove is formed in one side of the first positioning block, the positioning groove is a square groove, and a convex edge matched with the positioning groove is convexly arranged on one side, butted with the first positioning block, of the second positioning block.

4. The kit for extracting DNA from whole blood by the magnetic bead method according to claim 3, wherein: the first positioning block or the second positioning block is in a triangular prism shape in overall shape.

5. The reagent kit for extracting DNA from whole blood by the paramagnetic particle method according to claim 4, wherein: the heights of the first positioning block and the second positioning block are the same, and the lengths of two right-angle sides of the first positioning block or the second positioning block are equal.

6. The reagent kit for extracting DNA from whole blood by the paramagnetic particle method according to claim 5, wherein: EP reagent pipe placing holes are formed in the tops of the first positioning block and the second positioning block, and the EP reagent pipe placing holes are blind holes.

7. The kit for extracting DNA from whole blood by the magnetic bead method according to claim 6, wherein: the depth of the EP reagent tube placing hole is more than or equal to half of the height of the first positioning block.

8. The kit for extracting DNA from whole blood by the magnetic bead method according to claim 7, wherein: the first positioning block and the second positioning block are in a square shape after being butted.

9. The kit for extracting DNA from whole blood by the magnetic bead method according to claim 8, wherein: the first positioning block and the second positioning block are made of rubber.

10. The kit for extracting DNA from whole blood by the paramagnetic particle method according to claim 9, wherein: handles are fixedly arranged at the tops of the first positioning block and the second positioning block.