CN212159300U

CN212159300U - Device for biological sample extraction, substrate for biological sample extraction, and biological sample extraction mechanism

Info

Publication number: CN212159300U
Application number: CN201922346127.XU
Authority: CN
Inventors: 夏永恒; 潘伟业; 李卓芮; 李志民; 李大为; 玄兆伶; 王海良; 王娟
Original assignee: Zhejiang Annoroad Biotechnology Co ltd
Current assignee: Annoroad Gene Technology Beijing Co ltd; Beijing Annoroad Medical Laboratory Co ltd
Priority date: 2019-12-24
Filing date: 2019-12-24
Publication date: 2020-12-15
Anticipated expiration: 2029-12-24

Abstract

The utility model discloses a device is used in biological sample extraction, the device is used in extraction includes: a substrate comprising a first plane; a magnetic bar, at least a portion of the magnetic bar protruding from the first plane; an extraction member comprising at least one extraction vessel having a closed end and an open end, the closed end being closer to the substrate first plane than the open end. The utility model also provides a base plate and biological sample extraction mechanism for biological sample extraction. The utility model provides a device is used in biological sample extraction, when using, place the magnetic bead in the extraction container, the magnetic bead can be adsorbed on the lateral wall of extraction container, the adsorbed height of magnetic bead both can not touched moving the liquid in-process moreover, also can not influence the elution of magnetic bead because the magnetic bead adsorbs highly too high moreover. The device for extracting biological samples is particularly suitable for an automatic liquid workstation.

Description

Device for biological sample extraction, substrate for biological sample extraction, and biological sample extraction mechanism

Technical Field

The utility model relates to a life science experimental facilities technical field, concretely relates to biological sample draws with device, be used for base plate and biological sample that biological sample drawed to draw mechanism.

Background

The kit for extracting DNA by the paramagnetic particle method is a comprehensive high-tech product of nanotechnology, molecular biology technology, biomedical technology and forensic technology. The kit can be widely applied to a plurality of fields such as genome research in molecular biology, molecular evolution research, genetic disease research in medicine, mutant gene detection, tumor screening, HPV detection, HLA typing, transplantation matching and the like, forensic biological sample blood spot, fine spot, hair, cigarette butt and other field evidences, judicial paternity test, blood relationship identification and the like to provide evidence, archaeology, biological tests in primary and middle schools, and the like. Compared with the traditional method, the magnetic bead method for extracting DNA kit is characterized by comprising the following steps: the Chelex100 method, the organic method, the silicon dioxide method, the salting-out method and the like are simpler and more convenient, the times of transferring the centrifugal tube are less, the pollution is not easy to occur, and the like. The magnetic bead method is irreplaceable in the aspects of DNA purification, micro-detection, PCR amplification and the like.

The magnetic separation process includes adding magnetic material capable of combining with the sample to be separated into solution containing the sample to be separated, applying outer magnetic field to the solution via the magnetic separating rack after the magnetic material is combined with the sample to be separated, and enriching the magnetic material combined with the sample to be separated from the solution under the action of the outer magnetic field to separate the sample. The existing magnetic separation frame generally comprises a main body frame and a magnetic plate, wherein the main body frame is provided with a centrifugal tube hole, and a magnet is embedded in the magnetic plate. When the centrifugal separation device is used, a centrifugal tube filled with a sample is placed into the hole, the magnetic material in the sample can be enriched on the centrifugal tube wall on one side of the magnetic plate under the action of a magnetic field, and the separation of the magnetic material can be realized by sucking liquid away by using a liquid transfer device.

However, there are some problems in the prior art in practical applications, in the prior art, magnetic beads are uniformly distributed around the reaction well, the magnetic beads in the reaction well are usually adsorbed on the sidewall and the bottom of the reaction well, and the magnetic beads at the bottom are often touched during pipetting, so that the magnetic beads are lost or the pipetting is not thorough.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a device for biological sample extraction and be used for base plate that biological sample drawed, device for biological sample extraction, when using, the magnetic bead can be adsorbed on the lateral wall of extraction container, and the adsorbed height of magnetic bead neither can be touched at the transfer liquid in-process moreover, also can not influence the elution of magnetic bead because the magnetic bead adsorbs highly too high. The device for biological sample extraction is particularly suitable for use in an automated liquid workstation, for example, the ANNOSTAR model.

To achieve the above object, according to one aspect of the present invention, there is provided a device for biological sample extraction.

A biological sample draw and use device, draw and use the device and include:

a substrate comprising a first plane;

a magnetic bar, at least a portion of the magnetic bar protruding from the first plane;

an extraction member comprising at least one extraction vessel having a closed end and an open end, the closed end being closer to the substrate first plane than the open end.

In an embodiment of the present invention, the first plane of the substrate is provided with an accommodating cavity; the magnetic rod includes an insertion portion inserted into the accommodation chamber and a protrusion portion protruding from the first plane.

In an embodiment of the present invention, the extraction member includes a support portion, the support portion is connected to the extraction container, and the substrate first plane is supported by the extraction container.

In an embodiment of the present invention, the base plate has a base plate fixture block, the base plate fixture block will the extraction member is restricted on the base plate.

The utility model discloses an in the embodiment, biological sample draws and uses device still includes the support, the support fixture block has on the support, the support supports the base plate, the support fixture block will draw the part restriction to be in on the base plate.

In an embodiment of the present invention, the magnetic rods are two or more.

In an embodiment of the present invention, the device for biological sample extraction includes at least one array group, the array group includes:

two rows of extraction vessels;

at least one column of magnetic bars located between the two columns of extraction vessels.

In the embodiment of the present invention, the magnetic rods and the position of the extraction container are arranged such that, when the extraction operation is performed, the positions of the magnetic rods related to the extraction container on the first plane of the substrate are non-centrosymmetrically distributed around the position of the extraction container projected onto the first plane of the substrate as the axis;

the magnetic bar closest to the projection position of the extraction container on the first plane of the substrate is the relevant magnetic bar of the extraction container.

Optionally, one said extraction vessel has at least two associated magnetic bars.

Optionally, the angle between all lines connecting the center of the position of the extraction container projected onto the first plane of the substrate and the center of the position of its associated magnetic bar on the first plane of the substrate is not more than 150 °, preferably not more than 120 °, preferably not more than 90 °.

In the embodiment of the present invention, along the protruding direction of the magnetic rod, the ratio of the length of the overlapped segment of the extraction container and the magnetic rod to the length of the remaining segment of the extraction container is 1:4 to 1: 6.

The utility model discloses an among the embodiment, the bar cross section of magnetism is the rhombus with holding the chamber cross section, it forms the rhombus on the first plane and holds the chamber entry to hold the chamber, the rhombus holds a diagonal perpendicular to base plate edge of at least rhombus in the chamber entry, and preferred rhombus is the square, the square holds the contained angle between the limit of square and the base plate edge in the chamber entry and is 45 degrees.

In an embodiment of the present invention, the side of the cross section of the accommodating chamber is arc-shaped.

To achieve the above object, according to one aspect of the present invention, there is provided a substrate for biological sample extraction.

The utility model provides a base plate for biological sample extraction, the base plate includes the first plane, the first plane includes extraction container placement position and holds the chamber, hold the setting of chamber and extraction container placement position, the position of the relevant chamber of holding of extraction container placement position on the first plane of base plate is with extraction container placement position is the axle center and is non-central symmetrical distribution;

the receiving chamber closest to the extraction container placement position is the associated receiving chamber of the extraction container placement position.

Optionally, one of the extraction container placement locations has at least two associated receiving cavities.

Optionally, the angle between all the lines connecting the center of the placement position of the extraction container and the center of the position of its associated receiving chamber in the first plane of the base plate is not more than 150 °, preferably not more than 120 °, preferably not more than 90 °.

In an embodiment of the present invention, the first plane has at least one array group thereon, the array group includes:

two rows of extraction container placement locations;

at least one row of receiving cavities located between the two rows of extraction container placement locations.

To achieve the above object, according to one aspect of the present invention, there is provided a biological sample extraction mechanism.

The utility model provides a biological sample extraction mechanism, which comprises a substrate and a magnetic bar; wherein

The substrate comprises a first plane, the first plane is provided with an accommodating cavity and an extraction container placing position, the accommodating cavity and the extraction container placing position are arranged, and the positions of the relevant accommodating cavities of the extraction container placing position on the first plane of the substrate are distributed in a non-centrosymmetric mode by taking the extraction container placing position as an axis;

At least a portion of the magnetic rod is inserted into the receiving cavity.

In an embodiment of the present invention, the first plane has at least one array group thereon, the array group includes: two rows of extraction container placement locations; at least one row of receiving cavities located between the two rows of extraction container placement locations.

According to the utility model provides a device is used in biological sample extraction, when using, with the magnetic bead place in the extraction vessel, the magnetic bead can be adsorbed on the lateral wall of extraction vessel, the adsorbed height of magnetic bead both can not touched at the liquid-transfering in-process moreover, also can not influence the elution of magnetic bead because the magnetic bead adsorbs highly too high moreover.

Drawings

The accompanying drawings are included to provide a better understanding of the present invention and are not intended to constitute an undue limitation on the invention. Wherein:

fig. 1 is a schematic side view of a biological sample extraction device according to the present invention;

fig. 2 is a schematic perspective view of the biological sample collection device according to the present invention;

fig. 3 is a schematic top view of the biological sample extraction device according to the present invention;

fig. 4 is a schematic plan view of the structure of the biological sample collection substrate according to the present invention.

List of reference numerals

1-support part, 2-bracket clamping block, 3-extraction container, 4-base plate, 5-open end, 6-bracket, 7-containing cavity and 8-magnetic rod.

Detailed Description

Exemplary embodiments of the invention are described below with reference to the accompanying drawings, in which various details of embodiments of the invention are included to assist understanding, and which are to be considered exemplary only. Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope and spirit of the invention. Also, descriptions of well-known functions and constructions are omitted in the following description for clarity and conciseness.

Referring to fig. 1-4, the present invention relates to a device for extracting a biological sample, the device for extracting comprising:

a substrate 4, the substrate 4 comprising a first plane;

a magnetic bar, at least a portion of the magnetic bar protruding from the first plane; i.e. the magnetic bar is partly inserted into the first plane and partly extends out of the first plane.

Extraction means comprising at least one extraction vessel 3, said extraction vessel 3 having a closed end and an open end 5, said closed end being closer to said first plane of the substrate 4 than said open end 5. I.e. the closed end of the extraction container 3 is close to the first plane and the open end 5 is remote from the first plane.

The substrate 4 may have a rectangular parallelepiped plate-like structure.

The utility model provides a device is used in biological sample extraction, when using, with the magnetic bead place in the extraction container 3, the magnetic bead can be adsorbed on the lateral wall of extraction container 3, the adsorbed height of magnetic bead both can not touched at the liquid-transfering in-process moreover, also can not influence because the magnetic bead adsorbs highly too high the elution of magnetic bead.

In the embodiment of the present invention, the first plane of the substrate 4 is provided with an accommodating cavity 7; the magnetic rod includes an insertion portion inserted into the accommodation chamber 7 and a protrusion portion protruding from the first plane.

The outer diameter of the insertion part is slightly smaller than the inner diameter of the accommodating cavity 7, so that the insertion part of the magnetic rod can be inserted into the accommodating cavity 7 and is not easy to fall off from the accommodating cavity 7.

The deeper the magnetic rod is inserted into the accommodating cavity 7, the more stable the magnetic rod is on the first plane of the substrate 4, and the less likely the magnetic rod falls.

The depth of the accommodating cavity 7 in the substrate 4 does not exceed the thickness of the substrate 4.

In the embodiment of the present invention, the extraction member includes a support portion 1, the support portion 1 is connected to the extraction container 3, and the substrate 4 is supported on the first plane by the extraction container 3.

In one embodiment, the supporting portion 1 is a cubic housing, the supporting portion 1 is fastened to a first plane of the substrate 4, and the supporting portion 1 covers the first plane.

In the embodiment of the present invention, the base plate 4 has a base plate 4 fastening block, and the base plate 4 fastening block restricts the extraction component on the base plate 4.

The base plate 4 fixture block encircles the edge of base plate 4 and sets up in succession, just base plate 4 fixture block with draw the support portion 1 lock connection of part.

The utility model discloses an in the embodiment, biological sample takes device still includes support 6, support fixture block 2 has on the support 6, support 6 supports base plate 4, support fixture block 2 will the extraction element restriction is in on the base plate 4.

The bracket fixture block 2 is arranged around the edge of the bracket 6, the height of the bracket fixture block 2 is larger than the sum of the thicknesses of the substrate 4 and the bracket 6, and the bracket fixture block 2 clamps the extracting component on the substrate 4, so that the extracting component can be fixed on the substrate 4, the extracting container is not easy to shift when the mechanical arm moves the magnetic frame, and the mechanical arm can accurately place the extracting container on the corresponding position on the substrate.

In one embodiment, the holder block 2 is discretely disposed around the edge of the holder 6, and the holder block 2 may be a bar. The number of the bracket fixture blocks 2 is at least 2.

In one embodiment, the holder block 2 is integrally disposed around the edge of the holder 6, and the holder block 2 may have a cubic frame-shaped structure.

In an embodiment of the present invention, the magnetic rods are two or more.

The number of the magnetic rods can be 2, 4, 6, 12, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 96 and the like.

The number of the accommodating cavities 7 is not less than that of the magnetic rods.

two rows of extraction vessels 3;

at least one row of magnetic bars, which is located between the two rows of extraction containers 3. From this, two are listed as extraction vessel 3 mainly are in the magnetic field of the magnetic force stick in the middle, promptly extraction vessel 3 only receives the attraction in a single direction magnetic field to when the sample was drawed, the magnetic bead that adsorbs the sample adsorbs on the lateral wall of extraction vessel 3 one side, with avoid touching the magnetic bead because of moving the liquid in-process, and the loss that causes has further improved extraction efficiency.

In a specific embodiment, the number of magnetic bars is 42, and the number of magnetic bars is 6 columns, 7 each, and the number of extraction containers 3 is 96. Specifically, the extraction container 3 may be a commercially available 96-well plate.

In one embodiment, the magnetic rods in each column are arranged at equal intervals, and the magnetic rods in the same column are also arranged at equal intervals.

In one embodiment, the accommodating cavities 7 in each column are arranged at equal intervals, and the accommodating cavities 7 in the same column are also arranged at equal intervals.

In one embodiment, the array group comprises two columns of extraction containers 3 and one column of receiving cavities 7, wherein the two columns of extraction containers 3 are respectively located at two sides of the one column of receiving cavities 7, i.e. the receiving cavities 7 are located between the two columns of extraction containers 3.

In one embodiment, the array group includes two rows of extraction containers 3 and two rows of receiving cavities 7, the two rows of extraction containers 3 are located between the two rows of receiving cavities 7, each extraction container 3 has two associated receiving cavities, the two associated receiving cavities are located on the same side, and a connection line between a projection of the two associated receiving cavities on the first plane and a projection of the center of the extraction container 3 on the first plane forms an isosceles triangle. Two adjacent extraction containers 3 on the same row share a related accommodating cavity, and a connecting line between the projection of the centers of two adjacent extraction containers 3 on the same row on the first plane and the projection of the related accommodating cavity shared by the two adjacent extraction containers on the same row on the first plane forms an isosceles triangle.

In the embodiment of the present invention, the positions of the magnetic rods and the extraction container 3 are set such that, when the extraction operation is performed, the positions of the magnetic rods related to the extraction container 3 on the first plane of the substrate 4 are distributed in a non-central symmetrical manner with the position of the extraction container 3 projected on the first plane of the substrate 4 as the axis;

the magnetic bar closest to the projection position of the extraction container 3 on the first plane of the substrate 4 is the relevant magnetic bar of the extraction container 3.

Optionally, one of said extraction containers 3 has at least two associated magnetic bars, the number of which may be 2, 3, 4, and so on.

Optionally, the angle between all the lines connecting the center of the position of said extraction container 3 projected onto the first plane of the substrate 4 and the center of the position of its associated magnetic bar on the first plane of the substrate 4 is not more than 150 °, preferably not more than 120 °, preferably not more than 90 °.

The included angle between all the connecting lines of the position center of the extraction container 3 projected onto the first plane of the substrate 4 and the position center of the magnetic rod related to the extraction container on the first plane of the substrate 4 can be 10 °, 20 °, 30 °, 40 °, 50 °, 60 °, 70 °, 80 °, 90 °, 100 °, 110 °, 120 °, 130 °, 140 °, 150 °.

The larger the angle between all the connecting lines of the center of the position of the extraction container 3 projected onto the first plane of the substrate 4 and the center of the position of its associated magnetic bar on the first plane of the substrate 4, the further the distance between the associated magnetic bars.

In the embodiment of the present invention, along the protruding direction of the magnetic rod, the ratio of the length of the overlapped segment of the extraction container 3 and the magnetic rod to the length of the remaining segment of the extraction container 3 is 1:4 to 1: 6.

In one embodiment, the ratio of the length of the overlapped section of extraction container 3 and magnetic rod to the length of the remaining section of extraction container 3 may be 1: 4.

In one embodiment, the ratio of the length of the overlapped section of extraction container 3 and magnetic rod to the length of the remaining section of extraction container 3 may be 1: 5.

In one embodiment, the ratio of the length of the overlapped section of extraction container 3 and magnetic rod to the length of the remaining section of extraction container 3 may be 1: 6.

The magnetic force stick is made by neodymium iron boron magnet, the magnetism of magnetic force stick is up to 250mT, so that the magnetic bead in the extraction container 3 can adsorb on the bottom lateral wall of extraction container 3, be convenient for move liquid and elute.

Usually the cross section of drawing container 3 is circular, adjacent four draw and have formed the rhombus space in the middle of the container 3, in order to make the magnetic rod forms stronger magnetic field, can with the magnetic rod sets up in this rhombus space, the utility model discloses an embodiment, the magnetic rod cross section with hold chamber 7 cross section and be the rhombus, it forms the rhombus on the first plane and holds chamber 7 entry to hold chamber 7, the rhombus holds chamber 7 entry in at least rhombus's a diagonal perpendicular to base plate 4 edge, preferred rhombus is the square, the square holds the contained angle between the limit of square and base plate 4 edge in the chamber 7 entry to be 45 degrees.

In one embodiment, the magnetic rod is a cuboid with a square upper and lower section.

The utility model provides a base plate 4 for biological sample draws, base plate 4 includes the first plane, the first plane includes extraction container place position and holds chamber 7, hold chamber 7 and extraction container place position's setting, the position on the first plane of base plate 4 of relevant chamber that holds of extraction container place position uses the extraction container place position to be the axle center and is the non-centrosymmetric distribution;

the receiving chamber 7 closest to the extraction container placement position is the relevant receiving chamber for this extraction container placement position.

Optionally, one of said extraction container deposit positions has at least two associated containing cavities, the number of which may be 2, 3, 4, 5, and so on.

Optionally, the angle between all the lines connecting the centre of the placement position of said extraction container and the centre of the position of its associated receiving cavity in the first plane of the base plate 4 is not more than 150 °, preferably not more than 120 °, preferably not more than 90 °.

In one embodiment, the angle between all lines connecting the center of the position of the extraction container and the center of the position of its associated receiving cavity on the first plane of the base plate 4 may be 10 °, 20 °, 30 °, 40 °, 50 °, 60 °, 70 °, 80 °, 90 °, 100 °, 110 °, 120 °, 130 °, 140 °, 150 °.

The larger the angle between all the lines connecting the center of the placement position of the extraction container and the center of the position of its associated receiving chamber on the first plane of the base plate 4, the further the distance between the associated receiving chambers.

two rows of extraction container placement locations;

at least one row of receiving cavities 7, said at least one row of receiving cavities 7 being located between said two rows of extraction container placement positions. Wherein the extraction container placing position is used for placing the extraction container 3, and the accommodating cavity 7 is used for placing the magnetic bar. From this, two are listed as extraction vessel 3 and mainly are in the magnetic field of the magnetic force stick in the middle, receive the attraction in a single direction magnetic field promptly to when the sample was drawed, the magnetic bead that adsorbs the sample adsorbs on the lateral wall of extraction vessel 3 one side, in order to avoid touching the magnetic bead because of moving the liquid in-process, and the loss that causes has further improved extraction efficiency.

In a specific embodiment, the array group comprises two columns of extraction container placement positions and one column of receiving cavities 7, wherein the two columns of extraction container placement positions are respectively located at two sides of one column of receiving cavities 7, namely, the receiving cavities 7 are located between the two columns of extraction container placement positions.

In a particular embodiment, the array group comprises two columns of extraction container placement positions and two columns of receiving cavities 7, the two columns of extraction container placement positions being located between the two columns of receiving cavities 7. Each extraction container placing position is provided with two related accommodating cavities which are positioned on the same side, and a connecting line between the projection of the two related accommodating cavities on the first plane and the projection of the center of the extraction container placing position on the first plane forms an isosceles triangle. Two adjacent extraction container placing positions on the same row share a related accommodating cavity, and a connecting line between the projection of the centers of the two adjacent extraction container placing positions on the same row on the first plane and the projection of the related accommodating cavity shared by the two adjacent extraction container placing positions on the same row on the first plane forms an isosceles triangle.

The utility model provides a biological sample extraction mechanism, which comprises a substrate 4 and a magnetic bar; wherein

The substrate 4 comprises a first plane, the first plane is provided with a containing cavity 7 and an extraction container placing position, the containing cavity 7 and the extraction container placing position are arranged, and the positions of the relevant containing cavities of the extraction container placing position on the first plane of the substrate 4 are distributed in a non-centrosymmetric mode by taking the extraction container placing position as an axis;

At least a portion of the magnetic rod is inserted into the receiving cavity 7.

The included angle between all connecting lines of the center of the placing position of the extraction container and the position center of the related accommodating cavity on the first plane of the substrate 4 can be 10 degrees, 20 degrees, 30 degrees, 40 degrees, 50 degrees, 60 degrees, 70 degrees, 80 degrees, 90 degrees, 100 degrees, 110 degrees, 120 degrees, 130 degrees, 140 degrees and 150 degrees.

In an embodiment of the present invention, the first plane has at least one array group thereon, the array group includes: two rows of extraction container placement locations; at least one row of receiving cavities 7, said at least one row of receiving cavities 7 being located between said two rows of extraction container placement positions.

Specifically, fig. 4 shows a schematic structural diagram of a top view of a substrate 4 in the device for extracting a biological sample according to an embodiment of the present invention, as shown in fig. 4, the substrate 4 is a rectangular plate-shaped structure, a 6 × 7 accommodating cavity 7 is opened on an upper surface of the substrate 4, and each accommodating cavity 4 is inserted into a magnetic rod 8 (as shown in fig. 1) during an extracting operation. Fig. 2 and 3 show a schematic perspective view and a schematic top view of a device for extracting a biological sample according to an embodiment of the present invention, wherein the extraction member is disposed on the substrate 4 during use. The extraction member comprises 12 x 8 extraction containers 3, each extraction container 3 having an open end at its upper end and a closed end at its lower end. The extraction containers 3 are supported by the support 1 and connected as a whole to form a 12 x 8 array arrangement. As shown in fig. 1, fig. 1 shows a schematic side structure diagram of an extraction device according to a specific embodiment of the present invention, a magnetic rod 8 is inserted into a containing cavity 7 formed in the upper surface of the substrate 4, and the magnetic rod 8 protrudes from the upper surface of the substrate 4. When the substrate 4 is extracted, the substrate is placed on a bracket 6, the bracket 6 is provided with bracket blocks 2 on the sides, the bracket 6 supports the substrate 4, and the bracket blocks 2 extend upwards from the periphery of the substrate 4, so that the extracting component is limited at the fixed position on the substrate 4. As can be seen from fig. 1, the extraction container 3 and the magnetic rods 8 are arranged in the direction of extension of the long side of the base plate 4 in abaabaabaabaabaabaabaabaabaabaabaabaabaabaabaabaababa (where a denotes a row of extraction containers and B denotes a row of magnetic rods), and the extraction container 3 and the magnetic rods 8 are arranged in the direction of extension of the short side of the base plate 4 in abaabaabaabaabaabaabaabaabaabaabaababa (where a denotes a row of extraction containers and B denotes a row of magnetic rods). With this arrangement, the extraction container 3 and the magnetic rods 8 are not aligned in a straight line but are arranged in a staggered manner in the longitudinal direction of the substrate 4 or in the lateral direction of the substrate 4. When extracting, place the magnetic bead in extraction container 3, based on the effect of dislocation arrangement's magnetic rod 8, have two with every extraction container 3 apart from nearest magnetic rod 8, and these two magnetic rods 8 are located extraction container 3 with one side, can form the attraction that the magnetic bead only received unilateral direction magnetic field finally, thereby when the sample is drawed, the magnetic bead that adsorbs the sample adsorbs on the lateral wall of extraction container 3 one side, in order to avoid touching the magnetic bead because of moving the liquid in-process, and the loss that causes, further improved extraction efficiency.

Although the embodiments of the present invention have been described above with reference to the accompanying drawings, the present invention is not limited to the above-described embodiments and application fields, and the above-described embodiments are illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may effect numerous modifications thereto without departing from the scope of the invention as defined by the appended claims.

Claims

1. An apparatus for extracting a biological sample, comprising:

a substrate comprising a first plane;

a magnetic bar, at least a portion of the magnetic bar protruding from the first plane; and

2. The device for sampling a biological sample according to claim 1, wherein the base plate has a receiving cavity formed on a first plane; the magnetic rod includes an insertion portion inserted into the accommodation chamber and a protrusion portion protruding from the first plane.

3. The device for extracting a biological sample according to claim 1, wherein the extraction member includes a support portion that is connected to the extraction container and supports the extraction container on the first plane of the base plate.

4. The device for extracting biological samples according to any one of claims 1 to 3, wherein the base plate has a base plate fixture that constrains the extracting member on the base plate.

5. The device for extracting a biological sample according to claim 4, further comprising a holder having a holder block thereon, the holder supporting the base plate, the holder block restraining the extraction member on the base plate.

6. The device for extracting biological samples according to any one of claims 1 to 3, wherein the number of the magnetic rods is two or more.

7. The device for extracting a biological sample according to any one of claims 1 to 3, wherein the device for extracting a biological sample comprises at least one array set comprising:

two rows of extraction vessels;

8. The apparatus for extracting biological samples according to any one of claims 1 to 3,

the magnetic force bars and the position of the extraction container are arranged in such a way that when extraction operation is carried out, the positions of the relevant magnetic force bars of the extraction container on the first plane of the substrate are distributed in a non-centrosymmetric way by taking the position of the extraction container projected onto the first plane of the substrate as an axis;

9. The device for extracting biological samples of claim 8, wherein one of the extraction containers has at least two associated magnetic bars.

10. The apparatus according to claim 8, wherein the angle between all lines connecting the center of the position of the extraction container projected onto the first plane of the base plate and the center of the position of the associated magnetic rod on the first plane of the base plate is not more than 150 °.

11. The apparatus for sampling a biological sample according to claim 10, wherein the angle between all lines connecting the center of the position of the extraction container projected on the first plane of the base plate and the center of the position of the magnetic rod related thereto on the first plane of the base plate is not more than 120 °.

12. The apparatus for sampling a biological specimen according to claim 11, wherein an angle between all lines connecting a center of a position of the extraction container projected on the first plane of the base plate and a center of a position of the magnetic rod related thereto on the first plane of the base plate is not more than 90 °.

13. The device for extracting a biological sample according to any one of claims 1 to 3, wherein a ratio of a length of a superposed segment of the extraction container and the magnetic rod to a length of a remaining segment of the extraction container in a protruding direction of the magnetic rod is 1:4 to 1: 6.

14. The device for extracting biological samples according to claim 2, wherein the cross section of the magnetic rod and the cross section of the accommodating cavity are both diamond-shaped, the accommodating cavity forms diamond-shaped accommodating cavity inlets on the first plane, and at least one diagonal line of the diamond-shaped accommodating cavity inlets is perpendicular to the edge of the substrate.

15. The device for extracting biological samples according to claim 14, wherein the rhombus is a square, and the angle between the side of the square in the entrance of the square accommodating chamber and the edge of the substrate is 45 degrees.

16. A base plate for biological sample extraction, wherein the base plate comprises a first plane, the first plane comprises an extraction container placing position and a containing cavity, the containing cavity and the extraction container placing position are arranged, and the positions of the relevant containing cavities of the extraction container placing position on the first plane of the base plate are distributed in a non-centrosymmetric manner by taking the extraction container placing position as an axis;

17. The base plate for biological sample extraction of claim 16, wherein one said extraction container placement location has at least two associated receiving cavities.

18. The base plate for biological sample extraction of claim 16, wherein the angle between all lines connecting the center of the placement position of the extraction container and the center of the position of its associated receiving cavity on the first plane of the base plate is not more than 150 °.

19. The base plate for biological sample extraction of claim 18, wherein the angle between the center of the placement position of the extraction container and all lines connecting the centers of the positions of the associated receiving cavities on the first plane of the base plate is not more than 120 °.

20. The base plate for biological sample extraction of claim 19, wherein the angle between the center of the placement position of the extraction container and all lines connecting the centers of the positions of the associated receiving cavities on the first plane of the base plate is not more than 90 °.

21. The substrate for biological sample extraction of claim 16, wherein the first plane has at least one array set thereon, the array set comprising:

two rows of extraction container placement locations;

22. A biological sample extraction mechanism, characterized in that the biological sample extraction mechanism comprises a substrate and a magnetic bar; wherein

23. The mechanism of claim 22, wherein one of said extraction container positions has at least two associated receiving cavities.

24. The mechanism of claim 22, wherein the center of the positioning of the extraction container is not more than 150 ° from all lines connecting the centers of the respective cavities in the first plane of the base plate.

25. The mechanism of claim 24, wherein the angle between the center of the positioning of the extraction container and the center of the associated well in the first plane of the base plate is not greater than 120 °.

26. The mechanism of claim 25, wherein the angle between the center of the positioning of the extraction container and the center of the positioning of its associated well in the first plane of the base plate is no greater than 90 °.

27. The mechanism of claim 22, wherein at least a portion of the magnetic wand is inserted into the receiving cavity.

28. The biological sample extraction mechanism of claim 22, wherein the first plane has at least one array set thereon, the array set comprising: two rows of extraction container placement locations; at least one row of receiving cavities located between the two rows of extraction container placement locations.