CN217878869U - Enzyme-labeling instrument light source system and enzyme-labeling instrument - Google Patents

Enzyme-labeling instrument light source system and enzyme-labeling instrument Download PDF

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Publication number
CN217878869U
CN217878869U CN202221952145.8U CN202221952145U CN217878869U CN 217878869 U CN217878869 U CN 217878869U CN 202221952145 U CN202221952145 U CN 202221952145U CN 217878869 U CN217878869 U CN 217878869U
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light source
mounting hole
microplate reader
optical fiber
filter
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CN202221952145.8U
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Chinese (zh)
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韩洪志
陈强
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HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
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HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
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Abstract

The utility model discloses an ELIASA light source system and ELIASA, ELIASA light source system that wherein disclose, include: the light source box, for the tubular structure of axial both ends trompil, optic fibre, the incident terminal surface of optic fibre aims at the trompil of the wherein one end trompil of light source box and towards the trompil of the other end, and the axial one end of light source box is located to light source inserted block subassembly, light source inserted block subassembly includes the fixing base, and the one end of fixing base is equipped with the circuit board, is equipped with the mounting hole of a plurality of central axes towards the incident terminal surface central authorities of optic fibre in the fixing base, installs a monochromatic LED in every mounting hole, and monochromatic LED's pin welding still is equipped with the narrowband filter with the corresponding wavelength of monochromatic LED in this mounting hole in every mounting hole. The utility model discloses the ELIASA still discloses adopts as before ELIASA light source system.

Description

Enzyme-labeling instrument light source system and enzyme-labeling instrument
Technical Field
The utility model relates to a ELIASA field, in particular to ELIASA light source system and ELIASA.
Background
The ELIASA is a microplate reader, the detection principle is enzyme-linked immunosorbent assay, the enzyme-linked immunosorbent assay is carried out by an enzyme-catalyzed chromogenic substrate coupled on an antigen or an antibody, light waves emitted by a light source of the ELIASA are changed into a beam of monochromatic light through an optical filter or a monochromator, the monochromatic light is split by optical fibers and respectively enters a sample to be detected in a microplate, the reaction result is displayed by color, and the concentration of the antibody or the antigen to be detected in the sample can be judged according to the depth of color development, namely the magnitude of absorbance value; therefore, the light source filtering system of the microplate reader is one of the important components for realizing the detection function of the microplate reader.
The existing microplate reader filtering system disclosed in chinese patent with publication number CN212808022U comprises: a light source assembly including a lamp body having a light source therein; the light filtering assembly comprises a light filtering front cover, a light filtering rear cover, a light filtering wheel and a rotary driving mechanism, the light filtering front cover and the light filtering rear cover are detachably covered, a light filtering cavity is formed in the light filtering front cover, an aperture diaphragm communicated with the light filtering cavity is formed in the light filtering front cover, the lamp body is installed on the light filtering front cover, the light source is arranged corresponding to the aperture diaphragm, the light filtering wheel comprises a wheel body and a plurality of light filters, the wheel body is rotatably installed in the light filtering cavity, the rotating axis of the wheel body is located on one side of the aperture diaphragm, a plurality of light filtering holes are formed in the wheel body, the light filters are detachably installed in the light filtering holes in a one-to-one correspondence mode, the distance from the center of each light filtering hole to the rotating axis is equal to the distance from the center of the aperture diaphragm to the rotating axis, and the rotary driving mechanism is used for driving the wheel body to rotate; the light source component further comprises a lamp holder, a lamp cup and a lamp cover, the lamp holder is installed on the filtering front cover, the lamp cup is arranged in the lamp holder, the bottom of the lamp cup is right opposite to the filtering front cover, the lamp body is installed at the bottom of the lamp cup, and the lamp cover is arranged at the edge of the lamp cup.
The microplate reader filtering system provided by the above patent realizes the replacement of the filter by rotation, and although the step of switching the filter is simplified, the replacement step is still complicated when the filter is damaged and needs to be replaced.
SUMMERY OF THE UTILITY MODEL
For solving the problem that exists among the prior art, the utility model provides an ELIASA light source system.
A microplate reader light source system, comprising:
the light source box is a tubular structure with holes at two axial ends,
an optical fiber, the incident end face of the optical fiber is aligned with the opening at one end of the light source box and faces the opening at the other end,
the light source plug block assembly is arranged at one axial end of the light source box and comprises a fixed seat, a circuit board is arranged at one end of the fixed seat, a plurality of mounting holes with central axes facing the center of the incident end face of the optical fiber are formed in the fixed seat, a monochromatic LED is installed in each mounting hole, pins of the monochromatic LEDs are welded on the circuit board, and a narrow-band light filter with the wavelength corresponding to that of the monochromatic LED in each mounting hole is further arranged at one end, away from the circuit board, of each mounting hole.
Specifically, compared with the traditional halogen lamp with high energy consumption, large heat productivity and short service life, the single-color LED has the advantages of low energy consumption, small heat productivity, long service life, small volume and quick replacement; the wavelengths of the monochromatic LEDs installed in the installation holes are different, and each monochromatic LED is correspondingly provided with a narrow-band optical filter with the corresponding wavelength, so that the monochromatic LEDs with the adaptive wavelengths can be turned on according to the wavelength of the monochromatic light required by the specimen to be detected, and the optical filters do not need to be switched; meanwhile, the central axis of the mounting hole faces the incident end face of the optical fiber, so that light rays of the monochromatic LED mounted in the mounting hole can penetrate through the narrow-band filter and the light source box and are collected to the incident end face of the optical fiber after the monochromatic LED is turned on.
Preferably, the side wall of the light source box is provided with a fixing hole formed by extending outwards at an opening at one end of the optical fiber, and the incident end of the optical fiber extends into the fixing hole and is fixed by a lateral tightening screw which penetrates through the fixing hole and abuts against the optical fiber.
Specifically, under this kind of structure, relative motion is difficult to take place between optic fibre and the light source box, and the position of optic fibre is more stable.
Preferably, the fixed seat is provided with 1-6 mounting holes, and at least one of the mounting holes is internally provided with a single-color LED; when a plurality of single-color LEDs are mounted, the wavelength of each single-color LED is different.
Specifically, the number of the installed single-color LEDs can be reasonably selected according to the requirement of the specimen to be detected, so that at least one single-color LED is installed in the fixing seat, and at most, the single-color LEDs with the same number as the installing holes are installed.
Preferably, the included angle between the center point of the monochromatic LED light source and the normal of the center point of the incident end face of the optical fiber is less than or equal to half of the divergence angle of the optical fiber.
Specifically, under the condition of the angle, the light can be ensured to be better emitted into the optical fiber, and the light emitted into the optical fiber can be totally reflected under the angle.
Preferably, the mounting holes include an LED mounting hole facing one side of the circuit board and used for mounting a monochromatic LED, and an optical filter mounting hole facing away from one side of the circuit board and used for mounting a narrow-band optical filter, and a diaphragm hole with a reduced aperture is formed between the LED mounting hole and the optical filter mounting hole.
Specifically, light emitted by the monochromatic LED passes through the diaphragm hole along the center line of the light source and then passes through the narrow-band filter to be converged on the incident end face of the light.
Preferably, the light source inserting block assembly is further provided with a light filter pressing plate for preventing the narrow-band light filter from falling out of the light filter mounting hole on the side where the light filter mounting hole is located, and the light filter pressing plate is provided with avoiding holes which correspond to the light filter mounting hole in a one-to-one manner and allow detection light to pass through when in use.
Preferably, the circuit board is fixed to the fixing base through a first screw, and the optical filter pressing plate is fixed to the fixing base through a second screw.
Preferably, the peripheral surface of the fixed seat is provided with a step structure, wherein one end with a smaller outer diameter extends into the light source box, and the other end with a larger outer diameter is positioned outside the light source box; the fixing seat is detachably connected and fixed with the light source box.
Specifically, the fixing base and the light source box adopt a detachable connection mode, and the monochromatic LED and the narrow-band filter are fixed in the fixing base, so that the fixing base is detached and then other monochromatic LEDs with different wavelengths are mounted, and replacement of the monochromatic LEDs with different wavelengths is realized.
Preferably, the fixing seat and the light source box are connected and fixed in the following way:
(1) A plurality of magnetic steels are respectively embedded on the step surface of the step structure and the end surface of the light source box, and the magnetic steels on the step surface correspond to the magnetic steels on the end surface of the light source box one by one;
(2) The outer peripheral surface of one end with smaller outer diameter is fixedly connected with the inner side wall of the light source box through the elastic sheet and the buckling groove in a matching way;
(3) The end with larger outer diameter is fixedly connected with the end face of the light source box through a screw.
A microplate reader adopts the microplate reader light source system.
Compared with the prior art, the utility model discloses an useful part lies in:
the fixing seat is provided with a plurality of mounting holes with central axes facing to the center of the incident end face of the optical fiber, so that light rays emitted by the monochromatic LEDs arranged in the mounting holes can form a light path facing to the incident end face of the optical fiber; meanwhile, each monochromatic LED is matched with a narrow-band optical filter with adaptive wavelength, the monochromatic LEDs with adaptive wavelength are correspondingly opened according to the requirement of a product to be detected, the optical filters do not need to be switched and replaced, and the device is simple to operate and convenient to detect.
Drawings
FIG. 1 is an exploded view of a microplate reader light source system provided by the present invention;
FIG. 2 is a schematic view of a full section of a microplate reader light source system provided by the present invention;
FIG. 3 is a schematic perspective view of a light source insert block assembly of the microplate reader light source system provided by the present invention;
fig. 4 is the internal structure diagram of the light source plug block assembly of the microplate reader light source system provided by the utility model.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1 and 2, the microplate reader light source system includes:
the light source box 10 is a tubular structure with holes at two axial ends,
an optical fiber 20, an incident end face 21 of the optical fiber 20 is aligned with one end opening of the light source box 10 and faces to the opening of the other end,
light source plug block subassembly 30 locates the axial one end of light source box 10, light source plug block subassembly 30 includes fixing base 31, and the one end of fixing base 31 is equipped with circuit board 32, is equipped with the mounting hole 33 of a plurality of central axes towards the incident end face 21 central authorities of optic fibre 20 in the fixing base 31, installs a monochromatic LED34 in every mounting hole 33, and the pin welding of monochromatic LED34 is on circuit board 32, and the one end of keeping away from circuit board 32 in every mounting hole 33 still is equipped with the monochromatic LED34 corresponding wavelength's in this mounting hole 33 narrowband optical filter 35.
The side wall of the light source box 10 has a fixing hole formed by extending outward at an opening of one end of the optical fiber 20, and the incident end of the optical fiber 20 extends into the fixing hole and is fixed by a lateral fastening screw 11 penetrating through the fixing hole and abutting against the optical fiber 20.
With this structure, relative movement between the optical fiber 20 and the light source housing 10 is less likely to occur, and the position of the optical fiber 20 is more stable.
As shown in fig. 3 and 4, 4 mounting holes 33 are provided on the fixing base 31, and a monochromatic LED34 is mounted in at least one of the mounting holes 33; when a plurality of single-color LEDs 34 are mounted, the wavelength of each single-color LED34 is different.
The number of the single-color LEDs 34 can be reasonably selected according to the requirement of a specimen to be detected, so that at least one single-color LED34 is arranged in the fixed seat 31, and at most the single-color LEDs 34 with the same number as the mounting holes 33 are arranged.
The included angle between the center point of the light source of the monochromatic LED34 and the normal line of the center point of the incident end face 21 of the optical fiber 20 is less than or equal to half of the divergence angle of the optical fiber 20.
Under such an angle condition, the light can be ensured to be better incident into the optical fiber 20, and the light incident into the optical fiber 20 can be totally reflected at such an angle.
In the present embodiment, a silica fiber is used, and the divergence angle of the silica fiber is about 25 °, so that the included angle between the center point of the light source of the monochromatic LED34 and the normal line of the center point of the incident end face 21 of the optical fiber 20 is less than or equal to 12.5 °.
The mounting holes 33 comprise LED mounting holes facing one side of the circuit board 32 and used for mounting the monochromatic LEDs 34, and filter mounting holes facing away from one side of the circuit board 32 and used for mounting the narrow-band filters 35, and diaphragm holes 36 with reduced diameters are formed between the LED mounting holes and the filter mounting holes.
Light emitted by the monochromatic LED34 passes through the diaphragm hole 36 along the center line of the light source and then passes through the narrow-band filter 35 to be converged on the incident end face 21 of the light.
The light source inserting block assembly 30 is further provided with a light filter pressing plate 37 for preventing the narrow-band light filter 35 from falling out of the light filter mounting hole on the side where the light filter mounting hole is located, and the light filter pressing plate 37 is provided with avoiding holes 38 which correspond to the light filter mounting hole one to one and allow detection light to pass through in use.
The circuit board 32 is fixed to the fixing base 31 by a first screw 41, and the filter pressing plate 37 is fixed to the fixing base 31 by a second screw 42.
The peripheral surface of the fixed seat 31 has a step structure, wherein one end with a smaller outer diameter extends into the light source box 10, and the other end with a larger outer diameter is positioned outside the light source box 10; the fixed seat 31 is detachably connected and fixed with the light source box 10.
The fixing base 31 and the light source box 10 are detachably connected, and the monochromatic LEDs 34 and the narrow-band filter 35 are both fixed in the fixing base 31, so that the replacement of the monochromatic LEDs 34 with different wavelengths is realized by mounting other monochromatic LEDs 34 with different wavelengths after the fixing base 31 is detached.
A plurality of magnetic steels 43 are respectively embedded on the step surface of the step structure and the end surface of the light source box 10, and the magnetic steels 43 on the step surface correspond to the magnetic steels 43 on the end surface of the light source box 10 one by one.
A microplate reader adopts the microplate reader light source system.
Compared with the traditional halogen lamp with high energy consumption, large heating value and short service life, the single-color LED34 has the advantages of low energy consumption, small heating value, long service life, small volume and quick replacement; the wavelengths of the monochromatic LEDs 34 installed in the installation holes 33 are different, and each monochromatic LED34 is correspondingly provided with the narrow-band optical filter 35 with the corresponding wavelength, so that when the specimen is used specifically, the monochromatic LEDs 34 with the adaptive wavelengths can be opened according to the wavelength of the monochromatic light required by the specimen to be detected, and the optical filters do not need to be switched; meanwhile, since the central axis of the mounting hole 33 faces the incident end face 21 of the optical fiber 20, the light of the monochromatic LED34 mounted in the mounting hole 33 is collected on the incident end face 21 of the optical fiber 20 after being turned on through the narrow band filter 35 and the light source box 10.

Claims (10)

1. A microplate reader light source system, characterized by comprising:
the light source box is a tubular structure with holes at two axial ends,
an optical fiber, the incident end face of the optical fiber is aligned with the opening at one end of the light source box and faces the opening at the other end,
the light source plug block assembly is arranged at one axial end of the light source box and comprises a fixing seat, a circuit board is arranged at one end of the fixing seat, a plurality of mounting holes with central axes facing the center of the incident end face of the optical fiber are formed in the fixing seat, a single-color LED is installed in each mounting hole, pins of the single-color LEDs are welded on the circuit board, and a narrow-band light filter with the wavelength corresponding to that of the single-color LED in each mounting hole is further arranged at one end, far away from the circuit board, of each mounting hole.
2. The microplate reader light source system of claim 1, wherein the side wall of the light source box has a fixing hole formed by extending outward at an opening at one end of the optical fiber, and the incident end of the optical fiber extends into the fixing hole and is fixed by a lateral tightening screw passing through the fixing hole and abutting against the optical fiber.
3. The microplate reader light source system of claim 1, wherein the fixing base is provided with 1-6 mounting holes, and at least one of the mounting holes is internally provided with a single-color LED; when a plurality of single-color LEDs are mounted, the wavelength of each single-color LED is different.
4. The microplate reader light source system of claim 1, wherein the included angle between the center point of the monochromatic LED light source and the normal of the center point of the incident end face of the optical fiber is less than or equal to half of the divergence angle of the optical fiber.
5. The microplate reader light source system of claim 1, wherein the mounting holes comprise an LED mounting hole facing the circuit board for mounting a monochromatic LED, and a filter mounting hole facing away from the circuit board for mounting a narrowband filter, and a diaphragm hole with a reduced aperture is formed between the LED mounting hole and the filter mounting hole.
6. The microplate reader light source system of claim 5, wherein the light source plug block assembly is further provided with a filter pressing plate for preventing the narrowband filter from falling out of the filter mounting hole on the side where the filter mounting hole is located, and the filter pressing plate is provided with avoidance holes which correspond to the filter mounting holes one to one and through which detection light passes when in use.
7. The microplate reader light source system of claim 6, wherein the circuit board is fixed to the holder by a first screw, and the filter pressing plate is fixed to the holder by a second screw.
8. The microplate reader light source system of claim 1, wherein the peripheral surface of the fixing seat has a step structure, wherein the end with the smaller outer diameter extends into the light source box, and the end with the larger outer diameter is positioned outside the light source box; the fixing seat is detachably connected and fixed with the light source box.
9. The microplate reader light source system of claim 8, wherein the fixing seat and the light source box are fixedly connected in one of the following manners:
(1) A plurality of magnetic steels are respectively embedded on the step surface of the step structure and the end surface of the light source box, and the magnetic steels on the step surface correspond to the magnetic steels on the end surface of the light source box one by one;
(2) The outer peripheral surface of one end with smaller outer diameter is matched, connected and fixed with the inner side wall of the light source box through the elastic sheet and the buckling groove;
(3) The end with larger outer diameter is fixedly connected with the end face of the light source box through a screw.
10. A microplate reader characterized by comprising the microplate reader light source system according to claims 1 to 9.
CN202221952145.8U 2022-07-27 2022-07-27 Enzyme-labeling instrument light source system and enzyme-labeling instrument Active CN217878869U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202221952145.8U CN217878869U (en) 2022-07-27 2022-07-27 Enzyme-labeling instrument light source system and enzyme-labeling instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202221952145.8U CN217878869U (en) 2022-07-27 2022-07-27 Enzyme-labeling instrument light source system and enzyme-labeling instrument

Publications (1)

Publication Number Publication Date
CN217878869U true CN217878869U (en) 2022-11-22

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202221952145.8U Active CN217878869U (en) 2022-07-27 2022-07-27 Enzyme-labeling instrument light source system and enzyme-labeling instrument

Country Status (1)

Country Link
CN (1) CN217878869U (en)

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