CN217868808U

CN217868808U - Loop-mediated isothermal amplification chip

Info

Publication number: CN217868808U
Application number: CN202222147417.3U
Authority: CN
Inventors: 颜菁; 程林; 邹长华
Original assignee: Jiangsu Huixian Pharmaceutical Technology Co ltd
Current assignee: Jiangsu Huixian Pharmaceutical Technology Co ltd
Priority date: 2022-08-16
Filing date: 2022-08-16
Publication date: 2022-11-22
Anticipated expiration: 2032-08-16

Abstract

The utility model discloses a loop-mediated isothermal amplification chip, which comprises a chip body, wherein the surface of the chip body is provided with a sample adding port and a plurality of air vents; the loop-mediated isothermal amplification chip further comprises a sealing cover which is separated from the sample addition port and the air vent or covers the sample addition port and the air vent; the LAMP chip further comprises a hinge part connected between the chip body and the sealing cover, the sealing cover is integrally arranged on the chip body through the hinge part, and the hinge part has elasticity to allow the sealing cover to turn around the hinge part relative to the chip body. The loop-mediated isothermal amplification chip of the utility model has convenient use and simple structure.

Description

Loop-mediated isothermal amplification chip

Technical Field

The utility model particularly relates to a loop-mediated isothermal amplification chip.

Background

The loop-mediated isothermal amplification technology is widely applied to the field of biological diagnosis due to the advantages of mild reaction conditions (low reaction temperature), short reaction time and the like, for example, the nucleic acid amplification detection is carried out to diagnose whether pathogens exist in a sample. The loop-mediated isothermal amplification technology is a process of providing in-vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding fluorescent dye or fluorescent markers in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and analyzing the fluorescent signal to obtain a nucleic acid amplification result. The micro-fluidic chip is one of the important components of LAMP detection, and usually a corresponding LAMP detector needs to be designed for a specific micro-fluidic chip, and when a nucleic acid amplification reaction is performed, the micro-fluidic chip is placed in the LAMP detector, and a reaction chamber of the micro-fluidic chip is heated, illuminated, detected and the like. After the sample adding is completed, a reaction bin and the like in the microfluidic chip need to be sealed so that the reaction can be carried out in a closed space, and the sealing cover can be covered on the sample adding port and the exhaust port to realize the sealing effect.

SUMMERY OF THE UTILITY MODEL

The utility model provides an improved loop-mediated isothermal amplification chip.

A loop-mediated isothermal amplification chip comprises a chip body, wherein a sample adding port and a plurality of air exhaust ports are formed in the surface of the chip body; the loop-mediated isothermal amplification chip further comprises a sealing cover which is separated from the sample adding port and the air outlet or covers the sample adding port and the air outlet; the LAMP chip further comprises a hinge part connected between the chip body and the sealing cover, wherein the sealing cover is integrally arranged on the chip body through the hinge part, and the hinge part has elasticity so as to allow the sealing cover to turn around the hinge part relative to the chip body.

In one embodiment, the chip body has a first long side and a second long side opposite to each other, the hinge portion is connected to the first long side, the second long side is provided with a first connection portion, the sealing cover has a second connection portion, when the loop-mediated isothermal amplification chip is in an initial state, the first connection portion and the second connection portion are separated from each other, and the sealing cover is separated from the sample addition port and the air exhaust port; when the loop-mediated isothermal amplification chip is in a detection state, the first connecting part and the second connecting part are connected with each other, and the sealing cover covers the sample adding port and the air exhaust port.

In an embodiment, the first connecting portion includes a first lug, and the second connecting portion is clamped inside the first lug in the detection state.

In an embodiment, the second connecting portion comprises a first recess, the first lug being located in the first recess in the detection state.

In an embodiment, the sample addition port and the plurality of exhaust ports are disposed on the back surface of the chip body, the number of the sealing covers is one, and the sealing covers simultaneously seal the sample addition port and the plurality of exhaust ports.

In one embodiment, an overflow groove is further disposed on the surface of the chip body, the sample addition port is communicated with the overflow groove through a connection channel, and the depth of the connection channel is smaller than the depths of the overflow groove and the sample addition port.

In one embodiment, the surface of the connecting channel is coated with a hydrophilic agent layer.

In one embodiment, the chip body further includes a plurality of reaction chambers, each of the reaction chambers is respectively communicated with the sample injection port through a first microchannel, each of the first microchannels has one or more bending parts, and each of the reaction chambers is also respectively communicated with a corresponding exhaust port through a second microchannel; the first microchannel is also in communication with a volume adjustment chamber.

In one embodiment, the loop-mediated isothermal amplification chip further comprises a waterproof gas-permeable membrane which is arranged on the back surface of the chip body and covers the gas outlet and allows gas to pass through but not liquid to pass through; the loop-mediated isothermal amplification chip also comprises foam arranged on the sealing cover, and the sealing cover presses the foam on the sample adding port and/or the air exhaust port in the detection state.

In an embodiment, the bending portions include first bending portions and second bending portions, the first bending portions and the second bending portions are disposed in a staggered manner, and the adjacent first bending portions and the adjacent second bending portions are connected by a linear channel.

The utility model adopts the above scheme, compare prior art and have following advantage:

the utility model discloses a loop-mediated isothermal amplification chip, the setting has elastic hinge portion between chip body and the sealed lid, can set up the two an organic whole, and when needs sealed sample addition mouth and gas vent, sealed lid overturns around the relative chip body of hinge portion, and sealed lid is in order to make the reaction go on in the enclosure space on sample addition mouth and gas vent, only needs a sealed lid to just realize sealed function promptly, and the structure is comparatively simple and the assembly is also more convenient.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings required for the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic diagram of the back side of a loop-mediated isothermal amplification chip according to an embodiment of the present invention in an inspection state;

FIG. 2 is a schematic diagram of the back side of a loop-mediated isothermal amplification chip according to an embodiment of the present invention in an initial state;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

FIG. 4 is a schematic front view of a loop-mediated isothermal amplification chip according to an embodiment of the present invention in an initial state.

Wherein the content of the first and second substances,

1. a chip body; 11. a sample addition port; 12. an exhaust port; 13. an overflow trough; 14. a reaction bin; 15. a first long side; 16. a second long side; 161. a first connection portion; 17. a second lug; 18. a second groove; 2. a sealing cover; 21. a second connecting portion; 22. soaking cotton; 3. a hinge portion; 4. a connecting channel; 5. a first microchannel; 51. a first bent portion; 52. a second bent portion; 6. a second microchannel; 7. a volume adjustment chamber; 8. waterproof ventilated membrane.

Detailed Description

The following detailed description of the preferred embodiments of the present invention is provided to enable those skilled in the art to more easily understand the advantages and features of the present invention, with reference to the accompanying drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.

In the description of the present application, it is to be understood that the terms "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "inner" and "outer" etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the devices or elements referred to must have specific orientations, be constructed in specific orientations and operations, and thus, should not be construed as limiting the present application.

Referring to fig. 1 to 4, the loop-mediated isothermal amplification chip of the present embodiment includes a chip body 1, a sealing cover 2, and a hinge 3, wherein the hinge 3 is connected between the chip body 1 and the sealing cover 2.

Further, the chip body 1 is integrally formed in a plate shape, and the chip body 1, the sealing cover 2 and the hinge portion 3 are integrally formed of plastic, such as injection molding. Specifically, the sealing cap 2 is integrally provided on the chip body 1 through a hinge portion 3; the hinge portion 3 has elasticity to allow the sealing cover 2 to be turned around the hinge portion 3 with respect to the chip body 1.

Referring to fig. 1 and 2, a sample inlet 11, a plurality of air outlets 12, and a waterproof air-permeable membrane 8 are disposed on the surface of the chip body 1. The waterproof gas-permeable membrane 8 covers the gas vent 12 to allow gas to pass through but not liquid to pass through, and the sealing lid 2 is detached from the sample port 11 and the gas vent 12 or is covered on the sample port 11 and the gas vent 12. Specifically, the loop-mediated isothermal amplification chip has an initial state and a detection state, and in the initial state, the sealing cover 2 is separated from the sample addition port 11 and the exhaust port 12; in the detection state, the sealing cap 2 is turned over with respect to the chip body 1 by bypassing the hinge portion 3 and covers the sample addition port 11 and the exhaust port 12.

More specifically, sample addition port 11 and a plurality of gas vent 12 set up in the back of chip body 1, and the quantity of sealed lid 2 is one, and when detecting the state, just can seal sample addition port 11 and a plurality of gas vent 12 simultaneously through a sealed lid 2 so that the reaction goes on in the enclosure space, and the structure is comparatively simple, and it is also more convenient to operate.

The chip body 1 has a first long side 15 and a second long side 16, and the first long side 15 and the second long side 16 are disposed opposite to each other. The hinge portion 3 is connected to the first long side 15, the first connecting portion 161 is disposed on the second long side 16, and the sealing cover 2 has a second connecting portion 21, specifically, the first connecting portion 161 is a first lug, and the second connecting portion 21 is a first groove.

Further, in the initial state of the loop-mediated isothermal amplification chip, the first connection portion 161 and the second connection portion 21 are separated from each other, and the sealing cap 2 is separated from the sample addition port 11 and the exhaust port 12; when the loop-mediated isothermal amplification chip is in a detection state, the first connecting part 161 and the second connecting part 21 are connected with each other, the sealing cover 2 covers the sample addition port 11 and the exhaust port 12, the loop-mediated isothermal amplification chip further comprises foam 22 arranged on the sealing cover 2, and the foam 22 can be adhered to the sealing cover 2 through an adhesive. In the detection state, the sealing cover 2 presses the foam 22 against the sample addition port 11 and the exhaust port 12, and the first groove is clamped inside the first lug, namely the first lug is positioned in the first groove.

Referring to fig. 3, an overflow groove 13 is further disposed on the surface of the chip body 1, the sample addition port 11 is communicated with the overflow groove 13 through a connection channel 4, and the depth of the connection channel 4 is smaller than the depths of the overflow groove 13 and the sample addition port 11, so that drainage is facilitated, and pollution to the back surface of the chip body 1 during sample addition is better avoided. Furthermore, the surface of the connecting channel 4 is covered with a hydrophilic reagent layer, so that the tension on the surface of water molecules can be rapidly reduced, and the sample overflowing from the sample port 11 can be rapidly guided to the overflow groove 13 through the connecting channel 4.

Referring to FIG. 3, the chip body 1 further includes a plurality of reaction chambers 14, wherein amplification reagents such as primers are pre-disposed in the reaction chambers 14, and the primers in different reaction chambers 14 may be the same or different to detect different pathogens. The whole chip body 1 or at least the part corresponding to the reaction chamber 14 is made of a material having a certain transparency (transparent or semitransparent) to allow laser light to enter and fluorescence to exit. Each reaction bin 14 is respectively communicated with the sample adding port 11 through a first microchannel 5, each reaction bin 14 is also respectively communicated with a corresponding gas exhaust port 12 through a second microchannel 6, each first microchannel 5 is respectively provided with one or more bent parts, the bent parts comprise a first bent part 51 and a second bent part 52, the first bent part 51 and the second bent part 52 are arranged in a staggered way, and the adjacent first bent part 51 and the second bent part 52 are connected through a linear channel. The first bending portion 51 and the second bending portion 52 are formed by intersecting a plurality of linear channels with different extending directions. The reaction chamber 14, the first microchannel 5 and the second microchannel 6 are arranged on the chip body 1. First microchannel 5 in this implementation still adjusts chamber 7 intercommunication with a volume, can be through the size that changes the volume in chamber 7 to the volume of cavity and runner in the nimble chip body 1 of adjusting.

Referring to fig. 2, the chip body 1 is further provided with a second protrusion 17, and the sealing cover 2 is provided with a second groove 18. Adding nucleic acid samples from the sample adding port 11, enabling the samples to enter the reaction bin 14, enabling the added samples to overflow into the overflow groove 13, enabling the sealing cover 2 to be overturned to the back face of the chip body 1 relative to the chip body 1 around the hinge portion 3, enabling the first connecting portion 161 and the second connecting portion 21 to be connected with each other, enabling the second lug 17 to be located in the second groove 18, enabling the foam 22 to be pressed on the sample adding port 11 and the waterproof breathable film 8 through the sealing cover 2, enabling a closed environment isolated from the outside air to be formed in the microfluidic chip, and conducting constant-temperature amplification and fluorescence detection in the closed environment. Further, a flange is provided on the back surface of the chip body 1, the sealing cap 2 is fitted between the flanges in the inspection state, and the first and second lugs 17 are formed on the flanges.

The waterproof breathable film 8 is arranged at the exhaust port 12 of the loop-mediated isothermal amplification chip of the embodiment, so that the exhaust function can be realized, and the liquid flow can be blocked, so that the automatic quantitative liquid supply function can be realized; the sample adding port 11 is provided with an overflow groove 13, and excessive liquid can overflow in the overflow groove 13 when liquid is added, so that the problem of liquid outflow caused by liquid adding errors can be solved; the sealing cover 2 is integrally arranged with the chip body 1 through the hinge part 3, the structure is simpler, and the assembly is more convenient.

As used in this specification and the appended claims, the terms "comprises" and "comprising" are intended to only encompass the explicitly identified steps and elements, which do not constitute an exclusive list, and that a method or apparatus may include other steps or elements. As used herein, the term "and/or" includes any combination of one or more of the associated listed items.

It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it can be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Furthermore, the description of the upper, lower, left, right, etc. used in the present invention is only relative to the mutual positional relationship of the components of the present invention in the drawings.

The above embodiments are only for illustrating the technical concept and features of the present invention, and are preferred embodiments, which are intended to enable persons skilled in the art to understand the contents of the present invention and to implement the present invention, and thus, the protection scope of the present invention cannot be limited thereby. All equivalent changes or modifications made according to the principles of the present invention should be covered within the protection scope of the present invention.

Claims

1. A loop-mediated isothermal amplification chip comprises a chip body, wherein a sample adding port and a plurality of air exhaust ports are formed in the surface of the chip body; the loop-mediated isothermal amplification chip further comprises a sealing cover which is separated from the sample adding port and the air outlet or covers the sample adding port and the air outlet; the LAMP chip is characterized by further comprising a hinge part connected between the chip body and the sealing cover, the sealing cover is integrally arranged on the chip body through the hinge part, and the hinge part has elasticity so as to allow the sealing cover to turn around the hinge part relative to the chip body.

2. The LAMP chip as claimed in claim 1, wherein the chip body has a first long side and a second long side opposite to each other, the hinge is connected to the first long side, the second long side is provided with a first connecting portion, the sealing cover has a second connecting portion, the first connecting portion and the second connecting portion are separated from each other when the LAMP chip is in an initial state, and the sealing cover is separated from the sample inlet and the air outlet; when the loop-mediated isothermal amplification chip is in a detection state, the first connecting part and the second connecting part are connected with each other, and the sealing cover covers the sample adding opening and the air outlet.

3. The LAMP chip as claimed in claim 2, wherein the first connecting portion comprises a first lug, and the second connecting portion is engaged with the inner side of the first lug in the detection state.

4. The LAMP chip as set forth in claim 3, wherein the second connecting portion comprises a first groove, and the first lug is located in the first groove in the detection state.

5. The LAMP chip as set forth in claim 1, wherein the sample application port and the plurality of air discharge ports are provided on the back surface of the chip body, the number of the sealing caps is one, and the sealing caps simultaneously seal the sample application port and the plurality of air discharge ports.

6. The LAMP chip as claimed in claim 1, wherein the chip body further comprises an overflow groove on the surface thereof, the sample application port and the overflow groove are connected by a connection channel, and the depth of the connection channel is smaller than the depths of the overflow groove and the sample application port.

7. The LAMP chip as claimed in claim 6, wherein the connection channel has a hydrophilic reagent layer coated on its surface.

8. The LAMP chip as set forth in claim 1, wherein the chip body further comprises a plurality of reaction chambers, each of the reaction chambers is connected to the sample inlet through a first microchannel, each of the first microchannels has one or more bending portions, and each of the reaction chambers is connected to a corresponding gas outlet through a second microchannel; the first microchannel is also in communication with a volume adjustment chamber.

9. The LAMP chip as set forth in claim 2, wherein the LAMP chip further comprises a waterproof gas-permeable membrane disposed on the rear surface of the chip body and covering the gas outlet port, allowing gas to pass therethrough and not allowing liquid to pass therethrough; the loop-mediated isothermal amplification chip also comprises foam arranged on the sealing cover, and the sealing cover presses the foam on the sample adding port and/or the exhaust port in the detection state.

10. The LAMP chip according to claim 8, wherein the bending portions comprise first bending portions and second bending portions, the first bending portions and the second bending portions are disposed alternately, and the adjacent first bending portions and second bending portions are connected by straight channels.