CN217077607U

CN217077607U - Micro-fluidic chip based on LAMP

Info

Publication number: CN217077607U
Application number: CN202220738185.6U
Authority: CN
Inventors: 颜菁; 孟润荣
Original assignee: Jiangsu Huixian Pharmaceutical Technology Co ltd
Current assignee: Jiangsu Huixian Pharmaceutical Technology Co ltd
Priority date: 2022-03-31
Filing date: 2022-03-31
Publication date: 2022-07-29
Anticipated expiration: 2032-03-31

Abstract

The utility model discloses a micro-fluidic chip based on LAMP, it includes chip body and chip shell, be equipped with sample addition port and a plurality of gas vent on the chip body, the chip shell includes shell main part and sealed ear of folding, the chip body is adorned in the shell main part, micro-fluidic chip has detection status and initial condition, when in the detection status sealed ear of folding and the shell main part interconnect and cover on the sample addition port and/or the gas vent; the sealing tab is disengaged from the sample addition port and the vent port in the initial state. The utility model discloses a micro-fluidic chip based on LAMP uses more conveniently and sealed effect better.

Description

Micro-fluidic chip based on LAMP

Technical Field

The utility model belongs to the technical field of the LAMP detects, a micro-fluidic chip based on LAMP is related to.

Background

LAMP (Loop-mediated isothermal amplification) technology is widely applied to the field of biological diagnosis, such as nucleic acid amplification detection to diagnose whether pathogens exist in samples due to the advantages of mild reaction conditions (lower reaction temperature), short reaction time and the like. The LAMP technology is a process of providing in vitro amplification conditions for nucleic acid fragments, performing exponential amplification on the nucleic acid fragments, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and analyzing the fluorescent signal to obtain a nucleic acid amplification result. The micro-fluidic chip is one of the important components of LAMP detection, and usually a corresponding LAMP detector needs to be designed for a specific micro-fluidic chip, and when a nucleic acid amplification reaction is performed, the micro-fluidic chip is placed in the LAMP detector, and a reaction chamber of the micro-fluidic chip is heated, illuminated, detected and the like. After the application of sample is accomplished, need seal reaction storehouse etc. in the micro-fluidic chip so that the reaction goes on in the enclosure space, generally will seal application of sample mouth, gas vent etc. through the post seal membrane on the chip at present, however this kind of mode is used inconvenient inadequately, and sealed effect is not ideal enough moreover.

SUMMERY OF THE UTILITY MODEL

The utility model provides a micro-fluidic chip based on LAMP, it is more convenient to use, and sealed effect is better.

A micro-fluidic chip based on LAMP comprises a chip body and a chip shell, wherein the chip body is provided with a sample adding port and a plurality of air exhaust ports, the chip shell comprises a shell main body and a sealing folding lug, the chip body is arranged in the shell main body, the micro-fluidic chip has a detection state and an initial state, and the sealing folding lug and the shell main body are mutually connected and cover the sample adding port and/or the air exhaust ports in the detection state; the sealing tab is disengaged from the sample addition port and the vent port in the initial state.

In an embodiment, the microfluidic chip further includes reaction chambers, each of the reaction chambers is respectively communicated with the sample injection port through a first microchannel, each of the first microchannels has one or more bending portions, and each of the reaction chambers is also respectively communicated with a corresponding gas exhaust port through a second microchannel. The reagent in each reaction tank can be kept not to interfere with each other under the condition of not configuring a control valve or a piston, so that pollution is avoided; because a control valve or a piston and the like are not configured, the whole volume of the microfluidic chip is smaller (can be as small as 34mm multiplied by 58mm multiplied by 3mm), so that the whole volume of the LAMP detector matched with the microfluidic chip is greatly reduced, and the LAMP detector is suitable for hand-held detection.

In an embodiment, the bending portion includes an arc-shaped bending portion which is integrally arc-shaped.

In an embodiment, the arc-shaped bending portion includes a first arc-shaped bending portion and a second arc-shaped bending portion, and a center of the first arc-shaped bending portion and a center of the second arc-shaped bending portion are located on two opposite sides of the first microchannel respectively.

In an embodiment, the plurality of first arc-shaped bending portions and the plurality of second arc-shaped bending portions are arranged in an interlaced manner, and adjacent first arc-shaped bending portions and second arc-shaped bending portions are directly connected or connected through a linear channel.

In one embodiment, the central angle of the arc formed by the arc bending part is greater than 90 degrees. Reagent backflow in the reaction bin is avoided through the bending part, and the LAMP chip can keep the reagents of all the reaction tanks not to interfere with each other under the condition that a control valve or a piston is not arranged, so that pollution is avoided.

In one embodiment, the number of the sample addition ports is one, and the chip body is further provided with an overflow groove which is communicated with the sample addition ports or surrounds the sample addition ports.

In an embodiment, the microfluidic chip further comprises a sealing pad, and the sealing folded lug presses the sealing pad onto the sample addition port and/or the gas exhaust port in the detection state.

In one embodiment, the gasket is provided on the sealing tab. Further, the sealing gasket is a foam pad.

In one embodiment, the case main body has a first connecting portion, and the sealing tab has a second connecting portion, the first connecting portion and the second connecting portion being connected in the detection state, and the first connecting portion and the second connecting portion being disconnected from each other in the initial state.

In an embodiment, the first connection portion includes a first lug, and the second connection portion is clamped between the first lug and the chip body in the detection state.

In one embodiment, the sealing tab is rotatably coupled to the housing body relative to the housing body.

In one embodiment, the sealing tab is connected to the housing body by a deformable frangible portion.

In one embodiment, the weak portion is a folding line extending in a long side direction or a short side direction of the chip body.

In an embodiment, the chip body is embedded in the case body, the case body is provided with a second lug, the chip body is provided with a second groove matched with the second lug, and the second lug is located in the second groove.

In one embodiment, the LAMP chip further includes a cover film that is provided on the back surface of the chip body and covers the gas discharge port, and that allows gas to pass therethrough but does not allow liquid to pass therethrough.

In one embodiment, the sample inlet and the air outlet are disposed on a first surface of the chip body, the reaction chamber is disposed on a second surface of the chip body, the microfluidic chip further includes a sealing film for sealing the reaction chamber, and the sealing film is disposed on the second surface.

In a preferred embodiment, the second surface of the chip body is provided with a plurality of grooves, and the grooves are covered by the sealing film.

Further, the first microchannel and the second microchannel are also provided on the second surface of the chip body and covered by the sealing film.

The utility model adopts the above scheme, compare prior art and have following advantage:

the LAMP-based microfluidic chip of the utility model has the advantages that the sealing hangers are separated from the sample adding port and the exhaust port in the initial state; when detecting the state, through with sealed hangers and shell main part interconnect, make sealed hangers lid at the sample addition mouth and/or on the gas vent, form one with the isolated airtight environment of outside air, carry out constant temperature amplification and fluorescence detection in this airtight environment, guarantee to have better and the more convenient of use of better sealed effect.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic front view of a microfluidic chip according to an embodiment of the present invention, in which a folded lug is disposed on a chip body;

fig. 2 is a schematic back view of a microfluidic chip according to an embodiment of the present invention, in which a folded lug is disposed on a chip body;

FIG. 3 is a schematic diagram of a chip body;

FIG. 4 is a schematic view of the backside of a chip;

fig. 5 is a schematic front view of the microfluidic chip in an initial state;

fig. 6 is a schematic front view of the microfluidic chip in an initial state, in which the cover film is not shown;

fig. 7 is a back view of the microfluidic chip in an initial state.

Wherein,

7. a microfluidic chip;

70. a chip body; 701. a sample addition port; 702. an overflow trough; 703. an exhaust port; 704. a reaction bin; 705. a first microchannel; 705a, a first arc bending part; 705b, a second arc bending part; 706. a second microchannel; 707. a groove; 709. a second groove; 71. coating a film; 73. a sealing film; 74. a chip case; 741. a housing main body; 741a, a first lug; 741b, a second lug; 742. sealing the folding lug; 742a, a first groove; 743. a fold line;

75. and a gasket.

Detailed Description

The following detailed description of the preferred embodiments of the invention, taken in conjunction with the accompanying drawings, enables the advantages and features of the invention to be more readily understood by those skilled in the art. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.

In the description of the present application, it is to be understood that the terms "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "inner" and "outer" etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.

Referring to fig. 1 to 7, the LAMP chip 7 of the present embodiment includes a chip body 70, and the chip body 70 is provided with a sample addition port 701, a plurality of reaction chambers 704, and a plurality of exhaust ports 703. Each reaction chamber 704 is connected to the sample port 701 through a first micro-channel 705, and each first micro-channel 705 has one or more bending parts. Each reaction chamber 704 is also communicated with a corresponding exhaust port 703 through a second microchannel 706. The LAMP chip 7 is a miniaturized microfluidic chip, does not need any piston or valve for controlling liquid flow, is small in size, and is suitable for being used in a handheld LAMP detector. The first microchannel 705 of each reaction chamber 704 is bent at one or more positions to form one or more bent portions, so that reagents and the like in the reaction chambers 704 can be effectively prevented from flowing back to the first microchannel 705 without arranging a valve or a piston, and pollution to other reaction chambers 704 is avoided. The reaction chambers 704 are provided with amplification reagents such as primers in advance, and the primers in different reaction chambers 704 may be the same or different to detect different pathogens. The thickness of the front portion of the chip body 70, where the sample addition port 701 is opened, is larger than the thickness of the other portions. The chip body 70, or at least the portion corresponding to the reaction chamber 704, is made of a material having a certain transparency (transparent or translucent) to allow laser light to be injected and fluorescence to be emitted.

The bending part comprises an arc bending part which is integrally arc-shaped, and the central angle of the arc bending part is greater than 90 degrees, preferably 170 to 190 degrees. Further, the arc bending portion includes a first arc bending portion 705a and a second arc bending portion 705b, a center of the first arc bending portion 705a is located on a right side thereof, and a center of the second arc bending portion 705b is located on a left side thereof. The first arc bent portion 705a and the second arc bent portion 705b each have a central angle of 180 degrees. As shown in fig. 5, the first curved bending portion 705a and the second curved bending portion 705b are disposed alternately, and the adjacent first curved bending portion 705a and the second curved bending portion 705b are directly connected or connected through a linear channel, that is, each first microchannel 705 has a wavy portion, which effectively prevents the reagents such as primers in the reaction chamber 704 from flowing backwards.

In another embodiment, the bending portion includes a plurality of first bending portions and a plurality of second bending portions, each of the first bending portions integrally forms an acute angle, and each of the second bending portions integrally forms an acute angle. The first bending portion and the second bending portion are disposed alternately, and adjacent first bending portions and second bending portions are directly connected or connected through a linear channel, that is, each first micro-channel 705 has a zigzag portion.

The chip body 70 is integrally formed in a plate shape, and is formed by integrally molding plastic, such as injection molding. The chip body 70 has a back surface, and in this embodiment, the sample loading port 701 and the air discharging port 703 are disposed on the back surface of the chip body 70, and the reaction chamber 704, the first microchannel 705, and the second microchannel 706 are disposed on the chip body 70.

The microfluidic chip 7 further includes a cover film 71 that is provided on the surface of the chip body 70 and covers the gas vent 703, allowing gas to pass but not liquid to pass. The back of the chip body 70 is also provided with an overflow groove 702 arranged around the sample port 701, so that the back of the chip body 70 is prevented from being polluted during sample adding.

The microfluidic chip 7 further includes a sealing film 73 for sealing the reaction chamber 704, the first microchannel 705, and the second microchannel 706, the sealing film 73 being provided over the front surface of the chip body 70. The chip body 70 is provided with a plurality of grooves 707, and the grooves 707 are specifically formed in regions where the reaction chamber 704, the first microchannel 705 and the second microchannel 706 are not formed, have different shapes, sizes and positions according to the shapes and positions of the reaction chamber 704, the first microchannel 705 and the second microchannel 706, and avoid the reaction chamber 704, the first microchannel 705 and the second microchannel 706. These grooves 707 serve to facilitate sealing of the sealing film 73 and the chip body 70, to prevent generation of bubbles after the film is attached, and to prevent the chip body 70 from being bent and deformed.

When the microfluidic chip 7 is shipped, the cover film 71 and the sealing film 73 are attached to appropriate positions of the chip body 70 in advance, and isothermal amplification reagents including primers and the like are placed in each reaction chamber 704 in advance.

The microfluidic chip 7 further includes a chip shell 74, and the edges of the periphery of the chip body 70 are respectively provided with a clamping positioning mechanism which is matched with the chip shell 74. The chip case 74 includes a case main body 741 and a sealing tab 742, and the chip body 70 is housed in the case main body 741. The microfluidic chip 7 has a detection state and an initial state, and in the detection state, the sealing folded lug 742 and the shell main body 741 are connected with each other and cover the sample addition port 701 and/or the air vent 703; in the initial state, sealing tab 742 is disengaged from sample port 701 and vent port 703. That is, in this embodiment, the sample addition port 701 and the vent port 703 are closed by the seal tab 742. Further, as shown in fig. 7, the microfluidic chip 7 further includes a gasket 75, and the gasket 75 is pressed against the sample inlet 701 and/or the air outlet 703 by the sealing flange 742 in the detection state. The gasket 75 is disposed on the sealing flange 742, and is adhered to the sealing flange 742 by an adhesive, for example. In this embodiment, the chip case 74 includes two sealing tabs 742, one sealing tab 742 is used for sealing the sample addition port 701, and the other sealing tab 742 is used for sealing the air vent 703 under the cover film 71.

The housing main body 741 has a first connecting portion, and the sealing tab 742 has a second connecting portion, which are connected to each other in the detection state and are disconnected from each other in the initial state. Specifically, the first connection portion includes a first tab 741a, and the second connection portion is caught between the first tab 741a and the chip body 70 in the test state. The second connecting portion further has a first recess 742a thereon for receiving the first lug 741 a.

The sealing tab 742 is rotatably connected to the housing main body 741. After the sample application is completed, the sealing tab 742 is turned over and pressed against the chip body 70. Further, the seal tab 742 is connected to the housing main body 741 through a deformable weak portion. Specifically in the present embodiment, the weak portion is a folding line 743 extending in the longitudinal direction of the chip body 70. The sealing tab 742 and the case main body 741 are formed by integral injection molding, and the folding line 743 is formed after injection molding and has a thickness smaller than that of the surrounding portion, so that it can be easily folded. In other embodiments, the sealing tab 742 may also be hinged to the housing body 741 by a hinge shaft.

The chip body 70 is embedded in the housing main body 741, the housing main body 741 is provided with second protrusions 741b at four sides thereof, the clamping and positioning mechanism specifically refers to second grooves 709 formed in the chip body 70 and engaged with the second protrusions 741b, and the second protrusions 741b are located in the second grooves 709.

As shown in fig. 5 to 7, initially, the front surface of the chip body 70 is covered with the sealing film 73, the air vent 703 on the back surface of the chip body 70 is covered with the covering film 71, the chip body 70 is embedded in the chip housing 74, and the sealing tab 742 of the chip housing 74 extends out of the housing main body 741 and is located beside the chip body 70. In use, as shown in fig. 1 and 2, the nucleic acid sample is added from the sample adding port 701 until the nucleic acid sample flows to the coating film 71 to seal the coating film 71, and even more added sample overflows to the overflow groove 702; the sealing tab 742 is turned over to the back of the chip body 70 along the folding line 743, and the edge portion of the sealing tab is pressed under the first tab 741a until the first tab 741a is clamped in the first groove 742a, which is convenient for use and the sealing pad 75 is pressed by the sealing tab 742 on the sample port 701 and the cover film 71, so that a closed environment isolated from the outside air is formed in the microfluidic chip 7, and the isothermal amplification and the fluorescence detection are performed in the closed environment.

The microfluidic chip 7 can keep the reagents of each reaction tank from interfering with each other under the condition of not configuring a control valve or a piston, so as to avoid pollution; only one sample port 701 needs to be loaded with sample, so that the use is convenient; because a control valve or a piston and the like are not configured, the whole volume of the microfluidic chip 7 is smaller (can be as small as 34mm multiplied by 58mm multiplied by 3mm), so that the whole volume of the LAMP detector matched with the microfluidic chip is greatly reduced, and the LAMP detector is suitable for hand-held detection.

As used in this specification and the appended claims, the terms "comprises" and "comprising" are intended to only encompass the explicitly identified steps and elements, which do not constitute an exclusive list, and that a method or apparatus may include other steps or elements. As used herein, the term "and/or" includes any combination of one or more of the associated listed items.

It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Furthermore, the description of the upper, lower, left, right, etc. used in the present invention is only relative to the mutual positional relationship of the components of the present invention in the drawings.

The above embodiments are only for illustrating the technical concept and features of the present invention, and are preferred embodiments, which are intended to enable persons skilled in the art to understand the contents of the present invention and to implement the present invention, and thus, the protection scope of the present invention cannot be limited thereby. All equivalent changes or modifications made according to the principles of the present invention are intended to be covered by the scope of the present invention.

Claims

1. A micro-fluidic chip based on LAMP comprises a chip body and a chip shell, wherein the chip body is provided with a sample adding port and a plurality of air exhaust ports, and the micro-fluidic chip is characterized in that the chip shell comprises a shell main body and a sealing folding lug, the chip body is arranged in the shell main body, the micro-fluidic chip has a detection state and an initial state, and the sealing folding lug and the shell main body are mutually connected and cover the sample adding port and/or the air exhaust ports in the detection state; the sealing tab is disengaged from the sample addition port and the vent port in the initial state.

2. The microfluidic chip according to claim 1, wherein the microfluidic chip further comprises reaction chambers, each of the reaction chambers is connected to the sample inlet through a first microchannel, each of the first microchannels has one or more bending portions, and each of the reaction chambers is further connected to a corresponding gas outlet through a second microchannel.

3. The microfluidic chip according to claim 2, wherein the bent portion comprises an arc-shaped bent portion having an overall arc shape.

4. The microfluidic chip according to claim 3, wherein the arc-shaped bent portion comprises a first arc-shaped bent portion and a second arc-shaped bent portion, and a center of the first arc-shaped bent portion and a center of the second arc-shaped bent portion are located at two opposite sides of the first microchannel; and/or the central angle of the arc formed by the arc-shaped bending part is larger than 90 degrees.

5. The microfluidic chip according to claim 1, wherein the number of the sample loading ports is one, and an overflow groove is further disposed on the chip body, and the overflow groove is connected to the sample loading ports or surrounds the sample loading ports.

6. The microfluidic chip according to claim 1, further comprising a sealing pad disposed on the sealing tab, wherein the sealing tab compresses the sealing pad against the sample loading port and/or the gas exhaust port in the detection state.

7. The microfluidic chip according to claim 1, wherein the case body has a first connecting portion, the sealing tab has a second connecting portion, the first connecting portion and the second connecting portion are connected in the detection state, and the first connecting portion and the second connecting portion are disconnected from each other in the initial state.

8. The microfluidic chip according to claim 7, wherein the first connection portion comprises a first lug, and the second connection portion is clamped between the first lug and the chip body in the detection state.

9. The microfluidic chip according to claim 1, wherein the sealing tab is connected to the case main body by a deformable weak portion, and the weak portion is a folding line extending in a long side direction or a short side direction of the chip body.

10. The microfluidic chip according to claim 1, wherein the chip body is embedded in the housing main body, a second protrusion is disposed on the housing main body, a second groove is disposed on the chip body, and the second protrusion is disposed in the second groove; and/or the microfluidic chip further comprises a covering film which is arranged on the back surface of the chip body and covers the gas outlet and allows gas to pass but not liquid to pass.