CN217809499U

CN217809499U - Immune cell culture bag convenient to sample

Info

Publication number: CN217809499U
Application number: CN202222098859.3U
Authority: CN
Inventors: 王荣; 张霞; 祁晓凌; 乔昆
Original assignee: Yunnan Neo Life Stem Cell Co ltd
Current assignee: Yunnan Neo Life Stem Cell Co ltd
Priority date: 2022-08-10
Filing date: 2022-08-10
Publication date: 2022-11-15
Anticipated expiration: 2032-08-10

Abstract

The utility model relates to an immune cell culture bag convenient to sample, which belongs to the field of biological experiments, and comprises a culture bag body, a communicating pipe, a sampling pipe and a flow stopping valve, wherein one end of the communicating pipe is communicated with the bottom of the culture bag body, the other end of the communicating pipe is communicated with the sampling pipe, the number of the sampling pipe is not less than 2, the side wall of the sampling pipe is of a folding fold structure, and the flow stopping valve is arranged at the joint of the communicating pipe and the culture bag body; the utility model discloses a sampling tube is connected with the integration of cultivateing the bag body, reduces the possibility that immune cell suspension and external environment contacted in the sample process, reduces immune cell suspension sample by bacterial contamination's risk, and every sampling tube all sets up independent immune cell suspension circulation route simultaneously, and when avoiding taking a sample many times, the sample of last time is remained and is caused the influence to this sample.

Description

Immune cell culture bag convenient to sample

Technical Field

The utility model belongs to the biological assay field, specific theory relates to an immune cell culture bag convenient to sample.

Background

Cell culture refers to a method of simulating the in vivo environment in vitro, allowing it to survive, grow, reproduce and maintain the main structure and function. With the rapid development of immunocytobiology and molecular biology, immunotherapy has become one of the important means for radiotherapy, chemotherapy and postoperative adjuvant therapy of tumor patients. The immune cell culture process has a large impact on immune cell biology.

In the existing immune cell culture process, immune cells are generally amplified by adopting an immune cell culture bag, and the immune cell culture bag is breathable, waterproof, large in gas exchange area and capable of supporting high-density growth of cells. In the immune cell culture process, need carry out a lot of sample detection to it, generally extract immune cell suspension from the sample connection of immune cell culture bag through the syringe at present, cause immune cell suspension by bacterial contamination easily in the sample connection, and sample at every turn and all go on through same sample connection, the immune cell suspension of last sample can be remained to the sample connection, thereby cause the influence to this sample quality of taking a sample, finally lead to the testing result inaccurate, can't provide effective support for immune cell's research and application.

Therefore, there is a need to provide an immune cell culture bag convenient for sampling, which reduces the risk of bacterial contamination during the sampling process of immune cell samples, and simultaneously avoids the influence of residual samples on the samples.

Disclosure of Invention

In order to overcome the problem that exists among the background art, the utility model provides an immune cell culture bag convenient to sample through cultivateing bag body and communicating pipe one end intercommunication, the integral structure of the communicating pipe other end and sampling tube intercommunication, when reducing the sample, the possibility of immune cell suspension and external environment contact reduces the risk that the sample was by bacterial contamination. The funnel-shaped communicating pipe is used for gathering the immune cell suspension, so that the influence of the surface tension of the immune cell suspension on sampling is avoided. By arranging the check valve, whether the immune cell suspension flows into the sampling tube or not is controlled according to whether sampling is needed or not. Through the folding fold structure of sampling tube lateral wall, make the sampling tube can be stretched, inside produces the suction for the speed in the immune cell suspension flows into the sampling tube.

In order to achieve the above purpose, the utility model is realized by the following technical scheme:

immune cell culture bag including cultivateing bag body, communicating pipe, sampling tube, flow stopping valve, communicating pipe one end and cultivation bag body bottom intercommunication, the other end and sampling tube intercommunication communicating pipe, 2 are no less than to sampling tube quantity, the lateral wall of sampling tube is folding fold structure, flow stopping valve sets up in communicating pipe and cultivation bag body junction.

Preferably, the communicating pipe is in a funnel shape, the funnel end of the communicating pipe is communicated with the sampling pipe, and the check valve is arranged at the joint of the funnel part and the straight pipe part of the communicating pipe.

Preferably, the communicating pipes are uniformly arranged along the bottom of the culture bag body.

Preferably, the immune cell culture bag further comprises a handle, and the handle is fixedly arranged at the bottom of the sampling tube.

The utility model has the advantages that:

1. the utility model discloses a cell culture bag body, communicating pipe, the integration of sampling tube are connected, reduce the possibility that the outside environment contacted in the sampling process of immune cell, reduce the risk that the immune cell suspension is by bacterial contamination.

2. The utility model discloses a funnel type connecting pipe avoids the influence that immune cell suspension surface tension caused the sample.

3. The utility model discloses a stop flow valve, whether control immune cell suspension flows into the sampling tube from the culture bag in.

4. The utility model discloses a folding fold structure of sampling tube lateral wall makes the sampling process in, and the sampling tube can be stretched, and the inside suction that produces of sampling tube accelerates immune cell suspension inflow sampling tube speed.

The terms in the upper and lower directions described in the present specification are used for convenience of describing the structure of the present invention, and the present invention is not limited by the terms in the directions in actual use.

Drawings

FIG. 1 is a structural diagram of the working state of the embodiment 1 of the present invention

FIG. 2 is a structural diagram of the non-operating state of the embodiment 1 of the present invention

FIG. 3 is a structural diagram of the working state of the embodiment 2 of the present invention

In the figure, 1-a culture bag body, 2-a communicating pipe, 3-a sampling pipe, 4-a check valve and 5-a handle.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the following will explain in detail a preferred embodiment of the present invention with reference to the accompanying drawings to facilitate understanding of the skilled person.

As shown in fig. 1-3, the immune cell culture bag comprises a culture bag body 1, a communicating pipe 2, sampling tubes 3 and a check valve 4, wherein one end of the communicating pipe 2 is communicated with the bottom of the culture bag body 1, the other end of the communicating pipe 2 is communicated with the sampling tubes 3, the number of the sampling tubes 3 is not less than 2, the side walls of the sampling tubes 3 are of a folding fold structure, and the check valve 4 is arranged at the joint of the communicating pipe 2 and the culture bag body 1; the communicating pipe 2 is funnel-shaped, the funnel end of the communicating pipe 2 is communicated with the sampling pipe 3, and the check valve 4 is arranged at the joint of the funnel part and the straight pipe part of the communicating pipe 2; the communicating pipes 2 are uniformly arranged along the bottom of the culture bag body 1; the immune cell culture bag further comprises a handle 5, and the handle 5 is fixedly arranged at the bottom of the sampling tube 3. In example 1 (as shown in fig. 1-2), one end of the communicating tube 2 is communicated with the bottom of the culture bag body 1, the check valve 4 is disposed at the joint of the communicating tube 2 and the culture bag body 1, the check valve 4 is in a closed state during the immune cell culture stage, the communication path between the communicating tube 2 and the culture bag body 1 is closed, the immune cell suspension does not flow into the sampling tube 3, and the folded fold structure of the sampling tube 3 can make the sampling tube 3 in a compressed state during the immune cell culture stage. During sampling, the check valve 4 is opened, the communication passage between the communication pipe 2 and the culture bag body 1 is opened, the immune cell suspension flows into the sampling pipe 3 through the communication pipe 2, in the flowing process of the immune cell suspension, the handle 5 is pulled by hand, the sampling pipe 3 is pulled away from the compression state, the inner space of the sampling pipe 3 is increased, the air pressure is changed, suction is generated on the cell suspension in the communication pipe 2 communicated with the sampling pipe 3, the speed of the cell suspension flowing into the sampling pipe 3 is accelerated, after enough immune cell suspension flows into the sampling pipe 3, the check valve 4 is closed, the communication pipe 2 is fused and sealed near the check valve 4, and then the check valve 4 is taken down to be continuously used in later immune cell culture. Immune cell culture phase, immune cell suspension is in cultivateing bag body 1, and the sampling stage, immune cell suspension follow in the cultivateing bag body 1 through communicating pipe 2 flow in sampling tube 3, during the fusing, the fusing seals and accomplishes together, and whole process immune cell suspension all can not contact with external environment, greatly reduced immune cell suspension by bacterial contamination's risk. Embodiment 2 (as in fig. 3), communicating pipe 2 is the funnel type, communicating pipe 2 funnel end communicates with sampling tube 3, because communicating pipe 2 funnel end opening is great, be difficult to close and fuse, consequently, flow stopping valve 4 sets up in communicating pipe 2 funnel part and straight tube part junction, during the fuse, fuse to 2 straight tubes of communicating pipe near

flow stopping valve

4, 2 funnel parts of communicating pipe can play the effect of assembling immune cell suspension, eliminate the direct inflow of immune cell suspension and communicate the condition that the straight tube probably produced surface tension, avoid the influence that immune cell suspension surface tension led to the fact the sample to communicating pipe.

The utility model discloses a working process: in the immune cell culture process, the flow stopping valve is in the closed state, when needs carry out sampling operation, open the flow stopping valve, the immune cell suspension flows into communicating pipe, the rethread flows into in the sampling tube communicating pipe, hold the handle, stretch the sampling tube, make the immune cell suspension flow into the sampling tube more fast, the volume of the immune cell suspension that flows into in the sampling tube satisfies the demand after, close the flow stopping valve, close the operation of fusing and sealing communicating pipe near the flow stopping valve, finally, take off the flow stopping valve on communicating pipe that will fuse, accomplish whole sampling process promptly. Sampling at every time can be carried out according to the same operation aiming at different communicating pipes and sampling pipes.

Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.

Claims

1. An immune cell culture bag convenient to sample which characterized in that: immune cell culture bag including cultivateing bag body (1), communicating pipe (2), sampling tube (3), flow stopping valve (4), communicating pipe (2) one end and cultivation bag body (1) bottom intercommunication, communicating pipe (2) other end and sampling tube (3) intercommunication, 2 are no less than in sampling tube (3) quantity, the lateral wall of sampling tube (3) is folding fold structure, flow stopping valve (4) set up in communicating pipe (2) and cultivation bag body (1) junction.

2. An immune cell culture bag for facilitating sampling according to claim 1, wherein: the communicating pipe (2) is in a funnel shape, the funnel end of the communicating pipe (2) is communicated with the sampling pipe (3), and the stop valve (4) is arranged at the joint of the funnel part and the straight pipe part of the communicating pipe (2).

3. An immune cell culture bag for facilitating the sampling according to claim 1, wherein: the communicating pipes (2) are uniformly arranged along the bottom of the culture bag body (1).

4. An immune cell culture bag for facilitating the sampling according to claim 1, wherein: the immune cell culture bag further comprises a handle (5), and the handle (5) is fixedly arranged at the bottom of the sampling tube (3).