CN217809457U

CN217809457U - Temperature control carrying platform for high-throughput single-cell sequencing

Info

Publication number: CN217809457U
Application number: CN202221138116.8U
Authority: CN
Inventors: 苗健; 邱匀彦; 储冬东; 陈涣林; 马艳红; 徐传来; 刘振红
Original assignee: Suzhou New Geyuan Biotechnology Co ltd
Current assignee: Suzhou New Geyuan Biotechnology Co ltd
Priority date: 2022-05-12
Filing date: 2022-05-12
Publication date: 2022-11-15
Anticipated expiration: 2032-05-12

Abstract

The utility model relates to the technical field of bioengineering, a high flux unicellular sequencing is with accuse temperature microscope carrier is disclosed. The temperature control microscope stage for high-throughput single-cell sequencing is used for accommodating a biochip for high-throughput single-cell sequencing and comprises a microscope stage body, a heating module and a cooling module, wherein an accommodating groove is formed in the microscope stage body, and the biochip is placed in the accommodating groove. The heating module is arranged at the bottom end of the accommodating groove and can heat the biological chip in the accommodating groove, and a temperature sensor is embedded in the heating module. The cooling module is arranged on one side, away from the accommodating groove, of the heating module and used for reducing the temperature of the heating module. Through setting up heating module and cooling module, can carry out temperature regulation to the unicellular turbid liquid in the biochip to improve the activity of cell, and then guarantee the accuracy of test result.

Description

Temperature control carrier for high-throughput single-cell sequencing

Technical Field

The utility model relates to a bioengineering technical field especially relates to a high flux unicellular sequencing is with accuse temperature microscope carrier.

Background

In recent years, the biochip technology has been developed rapidly, especially the microfluidic technology, the new material technology and the artificial intelligence technology, so that the biochip technology is gradually industrialized. The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on one chip, and automatically completes the whole analysis process. Due to the great potential of the micro-fluidic chip in the fields of biology, chemistry, medicine and the like, the micro-fluidic chip has been developed into a new research field crossing the disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like.

When the biochip is used for high-throughput single-cell sequencing, the single-cell suspension is injected into the biochip for reaction, and the high-throughput single-cell sequencing has high requirements on the activity of the single-cell suspension. In actual experimental sequencing, different reaction temperatures were required due to differences in sample type and single cell suspension preparation methods. Inappropriate temperature can result in low single cell activity or low cell number, thereby affecting the output of effective single cell number and finally causing inaccurate analysis and statistical results of the obtained single cell data.

Therefore, there is a need for a temperature-controlled carrier for high-throughput single-cell sequencing, which solves the above problems in the prior art.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a high flux is accuse temperature microscope carrier for sequence of unicellular, the not good and unsafe problem of test result that leads to of temperature control when having solved unicellular sequencing.

To achieve the purpose, the utility model adopts the following technical proposal:

a temperature-controlled carrier for high-throughput single-cell sequencing is used for accommodating a biochip for high-throughput single-cell sequencing, and comprises:

the biological chip detection device comprises a platform deck body, a detection unit and a detection unit, wherein the platform deck body is provided with a containing groove, and the biological chip is placed in the containing groove;

the heating module is arranged at the bottom end of the accommodating groove and can heat the biological chip in the accommodating groove, and a temperature sensor is embedded in the heating module; and

the cooling module is arranged on one side, away from the accommodating groove, of the heating module and used for reducing the temperature of the heating module.

As an alternative, a through hole is formed in the position, opposite to the accommodating groove, of the carrier body, and the heating module penetrates through the through hole and can be abutted against the biochip.

Alternatively, the heating module comprises a heat conduction block and a heating element, the heating element can heat the heat conduction block, and the heat conduction block is arranged in the through hole in a penetrating mode to abut against the biochip so as to transfer heat to the biochip.

Alternatively, the heating element is a PI heating film.

As an alternative, the heat conduction block includes a temperature control portion and a connection portion, the temperature control portion is limited in the through hole, the connection portion is connected to the bottom end of the temperature control portion and abuts against the bottom side of the carrier body, a first mounting groove is formed in the connection portion, and the PI heating film is attached to the inside of the first mounting groove.

Alternatively, the connecting portion is connected to the stage body by a fastener.

As an alternative, the connecting portion is further provided with a second mounting groove, and the temperature sensor is arranged in the second mounting groove.

As an alternative, the cooling module comprises:

a fan disposed toward the heating module.

As an alternative, the cooling module further comprises:

the fixed support is connected to the carrying platform body, and the fan is installed on the fixed support.

As an alternative, the stage body includes:

the fixed carrying platform is provided with a limiting groove; and

the fixed carrying platform is detachably arranged in the limiting groove, and the movable carrying platform is provided with the accommodating groove.

The utility model has the advantages that:

the temperature control loading platform for high-throughput single cell sequencing provided by the utility model heats the biological chip by arranging the heating module, provides proper temperature for single cell suspension in the biological chip, and enables single cells to keep higher activity, thereby improving the accuracy of single cell data analysis and statistical results; the temperature of the heating module is monitored by arranging a temperature sensor, so that the heating module can be stabilized at a certain temperature, and the single cell suspension is further ensured to be maintained at the required temperature; when the reaction temperature is required to be reduced, the heating module is adjusted, and the cooling module is opened to cool the heating module, so that the heating module can rapidly reduce the required temperature, and the sensitivity of temperature adjustment of the biochip is improved.

Drawings

Fig. 1 is a schematic perspective view of a temperature-controlled carrier according to the present invention;

FIG. 2 is a schematic cross-sectional view of the temperature-controlled stage according to the present invention;

fig. 3 is a schematic structural diagram of a heating module provided by the present invention.

In the figure:

100. a biochip;

1. a stage body; 11. fixing a carrying platform; 111. a limiting groove; 12. a movable carrying platform; 121. a containing groove;

2. a heating module; 21. a heat conducting block; 211. a temperature control part; 212. a connecting portion; 2121. a first mounting groove; 2122. a second mounting groove; 22. a heating element; 23. a temperature sensor;

3. a cooling module; 31. a fan; 32. and (7) fixing the bracket.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention. It should be further noted that, for the convenience of description, only some of the structures associated with the present invention are shown in the drawings, not all of them.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the recitation of a first feature "on" or "under" a second feature may include the recitation of the first and second features being in direct contact, and may also include the recitation of the first and second features not being in direct contact, but being in contact with another feature between them. Also, the first feature "on," "above" and "over" the second feature may include the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

In the description of the present embodiment, the terms "upper", "lower", "right", "left", and the like are used in the orientation or positional relationship shown in the drawings only for convenience of description and simplicity of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used only for descriptive purposes and are not intended to be limiting.

In order to perform high-throughput single cell sequencing, the single cell suspension needs to be controlled at a suitable temperature so that the cells maintain high activity. The existing temperature control mode is that single cell suspension is heated to a certain temperature and then transferred to a biochip, so that the temperature is not easy to control accurately, and the control of time cost and manufacturing cost is not easy to realize due to more process steps.

Therefore, as shown in fig. 1, the present embodiment provides a temperature control stage for high-throughput single-cell sequencing, which is used for accommodating a biochip 100 for high-throughput single-cell sequencing and can control the temperature of a single-cell suspension in the biochip 100. Specifically, the temperature control stage for high-throughput single-cell sequencing comprises a stage body 1, a heating module 2 and a cooling module 3, as shown in fig. 2, a containing groove 121 is formed in the stage body 1, and the biochip 100 is placed in the containing groove 121. Heating module 2 sets up in storage tank 121 bottom, can heat the biochip 100 in the storage tank 121, and the embedded temperature sensor 23 that is equipped with of heating module 2, temperature sensor 23 are used for measuring the temperature of heating module 2. The cooling module 3 is disposed on one side of the heating module 2 away from the accommodating groove 121, and the cooling module 3 is used for reducing the temperature of the heating module 2.

The heating module 2 is arranged to heat the biochip 100, so that a proper temperature is provided for the single cell suspension in the biochip 100, and the cells keep high activity, thereby improving the accuracy of single cell data analysis and statistical results; the temperature of the heating module 2 is monitored by arranging the temperature sensor 23, so that the heating module 2 can be stabilized at a certain temperature, and the single cell suspension is further ensured to be maintained at the required temperature; when the reaction temperature needs to be reduced, the heating module 2 is adjusted, and the cooling module 3 is opened to cool the heating module 2, so that the heating module 2 can be rapidly cooled to the required temperature, and the sensitivity of temperature adjustment of the biochip 100 is improved.

Further, as shown in fig. 2, the stage body 1 includes a fixed stage 11 and a movable stage 12, a limiting groove 111 is provided on the fixed stage 11, the movable stage 12 is detachably disposed in the limiting groove 111, a containing groove 121 is provided on the movable stage 12, the movable stage 12 directly bears the biochip 100, the containing groove 121 forms a limiting effect on the biochip 100, and it is not necessary to additionally provide a limiting member to position the biochip 100, so that the operation is convenient. Meanwhile, the limiting groove 111 has a limiting effect on the movable carrier 12, which is beneficial to the installation of the movable carrier 12. A plurality of movable stages 12 having accommodating grooves 121 of different sizes can be arranged on the same fixed stage 11, and the adaptive movable stages 12 can be replaced for the biochips 100 of different sizes, so that the universality of the stage body 1 is improved.

Further, referring to fig. 2, a through hole is formed at a position of the carrier body 1 facing the containing groove 121, and the heating module 2 penetrates through the through hole and can be abutted against the biochip 100, so as to heat the biochip 100 better.

Specifically, referring to fig. 2 and 3, the heating module 2 includes a heat conducting block 21 and a heating element 22, the heating element 22 can heat the heat conducting block 21, and the heat conducting block 21 is inserted into the through hole to abut against the biochip 100 to transfer heat to the biochip 100. The heating element 22 is preferably a PI (Polyimide) heating film, which has excellent heat conduction efficiency and is advantageous for improving temperature control accuracy.

Preferably, as shown in fig. 2 and fig. 3, the heat conducting block 21 includes a temperature controlling portion 211 and a connecting portion 212, the temperature controlling portion 211 is limited in the through hole, the connecting portion 212 is connected to the bottom end of the temperature controlling portion 211 and abuts against the bottom side of the stage body 1, a first mounting groove 2121 is disposed on the connecting portion 212, the PI heating film is attached in the first mounting groove 2121, the first mounting groove 2121 is disposed at a position close to the temperature controlling portion 211 and extends parallel to the bottom end surface of the fixed stage 11, and the PI heating film is attached to the upper side wall of the first mounting groove 2121 to heat the temperature controlling portion 211 more quickly. Preferably, the connecting portion 212 is L-shaped and is connected to the stage body 1 by a fastener to fix the heat conduction block 21. The connection part 212 is further provided with a second mounting groove 2122, and the temperature sensor 23 is disposed in the second mounting groove 2122 to better sense the temperature of the heat conduction block 21.

Further, referring to fig. 2, the cooling module 3 includes a fan 31 and a fixing bracket 32, wherein the fan 31 is disposed toward the heating module 2 to cool the heating module 2 by blowing air. Fixed holder 32 is connected to stage body 1, and fan 31 is attached to fixed holder 32, thereby fixing fan 31 to stage body 1. Specifically, fixed bolster 32 sets up to the U type, and two fixed bolsters 32 are connected respectively in fan 31's both sides, and accessible bolt is connected between fixed bolster 32 and the microscope carrier body 1, between fixed bolster 32 and the fan 31, and the simple operation just is comparatively firm.

It is to be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. Numerous obvious variations, rearrangements and substitutions will now occur to those skilled in the art without departing from the scope of the invention. This need not be, nor should it be exhaustive of all embodiments. Any modification, equivalent replacement or improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A temperature-controlled carrier for high-throughput single-cell sequencing, for accommodating a biochip (100) for high-throughput single-cell sequencing, the temperature-controlled carrier comprising:

the biological chip carrier comprises a carrier body (1), wherein a containing groove (121) is formed in the carrier body (1), and the biological chip (100) is placed in the containing groove (121);

the heating module (2) is arranged at the bottom end of the accommodating groove (121) and can heat the biological chip (100) in the accommodating groove (121), and a temperature sensor (23) is embedded in the heating module (2); and

the cooling module (3) is arranged on one side, away from the accommodating groove (121), of the heating module (2), and the cooling module (3) is used for reducing the temperature of the heating module (2).

2. The temperature-controlled stage for high-throughput single-cell sequencing according to claim 1, wherein a through hole is formed in a position of the stage body (1) facing the containing groove (121), and the heating module (2) penetrates through the through hole and can abut against the biochip (100).

3. The temperature-controlled microscope stage for high-throughput single-cell sequencing according to claim 2, wherein the heating module (2) comprises a heat conduction block (21) and a heating element (22), the heating element (22) can heat the heat conduction block (21), and the heat conduction block (21) is provided with the through hole to abut against the biochip (100) so as to transfer heat to the biochip (100).

4. The temperature-controlled carrier for high-throughput single-cell sequencing according to claim 3, wherein the heating element (22) is a PI heating membrane.

5. The temperature-controlled carrier for high-throughput single-cell sequencing according to claim 4, wherein the heat-conducting block (21) comprises a temperature-controlled portion (211) and a connecting portion (212), the temperature-controlled portion (211) is limited in the through hole, the connecting portion (212) is connected to the bottom end of the temperature-controlled portion (211) and abuts against the bottom side of the carrier body (1), a first mounting groove (2121) is formed in the connecting portion (212), and the PI heating film is attached to the inside of the first mounting groove (2121).

6. The temperature-controlled carrier for high-throughput single-cell sequencing according to claim 5, wherein the connecting portion (212) is connected to the carrier body (1) by a fastener.

7. The temperature-controlled carrier for high-throughput single-cell sequencing according to claim 5, wherein the connecting portion (212) is further provided with a second mounting groove (2122), and the temperature sensor (23) is disposed in the second mounting groove (2122).

8. The temperature-controlled carrier for high-throughput single-cell sequencing according to any one of claims 1 to 7, wherein the temperature-reducing module (3) comprises:

a fan (31), the fan (31) being disposed toward the heating module (2).

9. The temperature-controlled carrier for high-throughput single-cell sequencing according to claim 8, wherein the temperature-reducing module (3) further comprises:

the fixing support (32) is connected to the stage body (1), and the fan (31) is mounted on the fixing support (32).

10. The temperature-controlled stage for high-throughput single-cell sequencing according to claim 1, wherein the stage body (1) comprises:

the fixed carrying platform (11) is provided with a limiting groove (111); and

the fixed carrying platform (11) is detachably arranged in the limiting groove (111), and the movable carrying platform (12) is provided with the accommodating groove (121).